Rw1G034700

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0008946
Physical Location & Seq
Forward (+)
62624450 .. 62630711
6262 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G034700.1

Sequence Viewer

Length: 1581 bp
ATGGAATCCACTATTTCTTTATCGTCTTCCTCTCTTCTCCTACCTTCGCCTCGCCGCCGACTCGGCTCCAAATCCCAAGCTTCAATTTCGCCCCGTAACCTCCGCCGTCCGAGCCTCAAATCCAGAACCTTCCTCGTTCCGTCTCTTTCCCGCCAAAACCGGCCTATCCGATCTCAGAGCTCGAATCATCAGCCGCAGGACGACGGCTTCGTCCTCGAAGACGTTCCTCACTTGACCAATTTCCTCCCAAACTTGCCGTCATATCCGAATCCATTGCAACAGAGCCAAGCTTATGCAGTTGTCAAGAAAACTTTTGTGAGCCCTGCGGATGCGGTTGCGCAGAAAGTTGTTGTTCAAGATGATAGTCCTAGAGGGGTACATTTTCGACGTGCAGGGCCTCGGGAAAAGGTTTATTTCAAGTCGGAGGAAGTGCGTGCTTGTATAGTGACATGTGGGGGTTTATGCCCGGGGATCAATACTGTGATTCGAGAGATAGTATGTGGCTTGAGCTACATGTATGGTGTTGAGGATATACTTGGAATTGAGGGAGGATATAGAGGCTTTTATTCTAAAAATACATTAAAGTTGACACCTAAAGTTGTCAATGATATCCATAAGCGGGGTGGCACCTTTCTCTGCACTTCACGAGGAGGTCATGATACCAACAAGATAGTTGATAACATACAGGACCGGGGAATAAATCAGGTATACATAATTGGAGGTGATGGCACCCAAAAAGGAGCAGCTCTAATATATGAGGAAGTTGAGAAACGTGGTCTTCAAGTGGCAGTTGCTGGGGTTCCTAAAACAATTGACAATGATATTGCTGTGATAGACAAGTCTTTTGGTTTTGACACTGCTGTTGAAGAAGCTCAGAGGGCTATTAATGCTGCACACGTGGAGGTTGAAAGTGTGGAAAATGGAATCGGAATTGTCAAACTGATGGGAAGATTCAGTGGCTTCATTGCATTATATGCAACTTTGGCAAGTCGAGATGTGGATTGTTGCTTGATTCCAGAATCTCCATTCTATTTGGAAGGCCAGGGTGGGCTTTTCGAATTTATTGAACAGCGTCTTAAAGAGAATGGACATATAGTTATCGTAGTAGCAGAAGGAGCCGGGCAGGAGTATGTTGCCCAGCAAATCCATGCTGCTGGAGAGAAAGATGCATCTGGAAATAAGTTGCTACTAGATGTTGGTATATGGTTAACCCAAAAGATTAAGGATCATTTTACAAAAGGTCAGCAGATGGCGGTAAATATGAAATATATAGATCCAACATATATGATTCGAGCTGTTCCAAGTAATGCATCAGACAATATATACTGCACTCTTCTGGCTCATAGTGCTGTTCATGGGGCCATGGCTGGATACAGTGGTTTCACGGTTGGGCCTGTTAACAGCAGACATGCCTATATTCCAATTTCTCGTGTGACAGAGACGCAAAACACAGTGAAGTTGACAGATCGGATGTGGGCTAGACTTCTTGCATCGACGAATCAGCCTAGTTTTTTGGAGAGAAATGAGTTGCAGTCTAGAGTTGATCTGATCAACAGCAACTTGGAAAATACATCTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003872 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008443 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009536 GO:0009987 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0061615 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230
Pfam Domains
Protein Families
PFK

Protein Analysis

526

Amino Acids

57.71

Weight (kDa)

8.48

Isoelectric Point (pI)

48.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PFK PF00365 146 - 452 7.1e-61 Phosphofructokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 209
Acc16I TGCGCA 1 cut(s) 339
AccB1I GGYRCC 2 cut(s) 626, 728
AccI GTMKAC 1 cut(s) 708
AciI CCGC 8 cut(s) 55, 103, 151, 194, 326, 332, 619, 1253
AclWI GGATC 3 cut(s) 479, 1233, 1268
AcsI RAATTY 1 cut(s) 1058
AcvI CACGTG 1 cut(s) 898
AfaI GTAC 1 cut(s) 378
AfiI CCNNNNNNNGG 1 cut(s) 619
AflIII ACRYGT 3 cut(s) 449, 513, 895
AgsI TTSAA 7 cut(s) 84, 356, 418, 782, 866, 908, 1067
AjiI CACGTC 1 cut(s) 389
AjnI CCWGG 1 cut(s) 1041
AluBI AGCT 7 cut(s) 80, 180, 290, 510, 746, 872, 1295
AluI AGCT 7 cut(s) 80, 180, 290, 510, 746, 872, 1295
Alw21I GWGCWC 1 cut(s) 182
Alw26I GTCTC 2 cut(s) 147, 1433
AlwI GGATC 3 cut(s) 479, 1233, 1268
AlwNI CAGNNNCTG 1 cut(s) 794
Ama87I CYCGRG 2 cut(s) 399, 466
AoxI GGCC 5 cut(s) 161, 395, 1039, 1359, 1391
ApeKI GCWGC 3 cut(s) 743, 890, 1151
ApoI RAATTY 1 cut(s) 1058
ArsI GACNNNNNNTTYG 6 cut(s) 191, 223, 242, 274, 827, 859
AseI ATTAAT 1 cut(s) 885
Asp700I GAANNNNTTC 1 cut(s) 222
AspLEI GCGC 1 cut(s) 340
AspS9I GGNCC 4 cut(s) 395, 688, 1359, 1391
AsuC2I CCSGG 4 cut(s) 467, 468, 692, 1120
AsuHPI GGTGA 1 cut(s) 734
AsuII TTCGAA 1 cut(s) 1056
AvaI CYCGRG 2 cut(s) 399, 466
AvaII GGWCC 1 cut(s) 688
BaeI ACNNNNGTAYC 2 cut(s) 1363, 1396
BanI GGYRCC 2 cut(s) 626, 728
BanII GRGCYC 2 cut(s) 182, 323
BauI CACGAG 2 cut(s) 645, 1428
BbrPI CACGTG 1 cut(s) 898
BbsI GAAGAC 3 cut(s) 18, 225, 770
Bbv12I GWGCWC 1 cut(s) 182
BbvI GCAGC 3 cut(s) 755, 877, 1138
BccI CCATC 3 cut(s) 719, 937, 1243
BceAI ACGGC 3 cut(s) 90, 220, 241
BcgI CGANNNNNNTGC 2 cut(s) 256, 290
BciT130I CCWGG 1 cut(s) 1043
BciVI GTATCC 1 cut(s) 1364
BclI TGATCA 1 cut(s) 1548
BcnI CCSGG 4 cut(s) 467, 468, 692, 1120
BcoDI GTCTC 2 cut(s) 147, 1433
BfaI CTAG 5 cut(s) 369, 1190, 1479, 1506, 1536
BfuI GTATCC 1 cut(s) 1364
BglI GCCNNNNNGGC 1 cut(s) 63
BisI GCNGC 5 cut(s) 55, 194, 744, 891, 1152
BlsI GCNGC 5 cut(s) 56, 195, 745, 892, 1153
Bme1390I CCNGG 5 cut(s) 467, 468, 692, 1043, 1120
Bme18I GGWCC 1 cut(s) 688
BmeT110I CYCGRG 2 cut(s) 399, 466
BmgBI CACGTC 1 cut(s) 389
BmgT120I GGNCC 4 cut(s) 395, 688, 1359, 1391
BmiI GGNNCC 6 cut(s) 67, 628, 730, 801, 1117, 1360
BmrFI CCNGG 5 cut(s) 467, 468, 692, 1043, 1120
BmsI GCATC 5 cut(s) 319, 1156, 1178, 1319, 1499
BpiI GAAGAC 3 cut(s) 18, 225, 770
BpmI CTGGAG 1 cut(s) 1176
Bpu14I TTCGAA 1 cut(s) 1056
BpuEI CTTGAG 1 cut(s) 526
BpuMI CCSGG 4 cut(s) 467, 468, 692, 1120
BsaAI YACGTR 1 cut(s) 898
BsaJI CCNNGG 6 cut(s) 398, 466, 467, 691, 1042, 1362
Bsc4I CCNNNNNNNGG 1 cut(s) 619
Bse118I RCCGGY 1 cut(s) 159
Bse3DI GCAATG 2 cut(s) 272, 963
BseBI CCWGG 1 cut(s) 1043
BseDI CCNNGG 6 cut(s) 398, 466, 467, 691, 1042, 1362
BseGI GGATG 2 cut(s) 334, 1476
BseLI CCNNNNNNNGG 1 cut(s) 619
BseMI GCAATG 2 cut(s) 272, 963
BseMII CTCAG 2 cut(s) 188, 887
BseRI GAGGAG 1 cut(s) 663
BseXI GCAGC 3 cut(s) 755, 877, 1138
BseYI CCCAGC 2 cut(s) 794, 1137
BsgI GTGCAG 4 cut(s) 411, 622, 876, 1312
BshFI GGCC 5 cut(s) 163, 397, 1041, 1361, 1393
BshNI GGYRCC 2 cut(s) 626, 728
BsiHKAI GWGCWC 1 cut(s) 182
BsiHKCI CYCGRG 2 cut(s) 399, 466
BsiSI CCGG 4 cut(s) 160, 467, 691, 1119
BslI CCNNNNNNNGG 1 cut(s) 619
BsmAI GTCTC 2 cut(s) 147, 1433
BsmBI CGTCTC 2 cut(s) 147, 1433
BsnI GGCC 5 cut(s) 163, 397, 1041, 1361, 1393
BsoBI CYCGRG 2 cut(s) 399, 466
Bsp119I TTCGAA 1 cut(s) 1056
Bsp1286I GDGCHC 2 cut(s) 182, 323
Bsp143I GATC 7 cut(s) 170, 471, 1225, 1273, 1465, 1543, 1548
Bsp19I CCATGG 1 cut(s) 1362
BspACI CCGC 8 cut(s) 55, 103, 151, 194, 326, 332, 619, 1253
BspANI GGCC 5 cut(s) 163, 397, 1041, 1361, 1393
BspCNI CTCAG 2 cut(s) 187, 886
BspHI TCATGA 1 cut(s) 655
BspLI GGNNCC 6 cut(s) 67, 628, 730, 801, 1117, 1360
BspPI GGATC 3 cut(s) 479, 1233, 1268
BspT104I TTCGAA 1 cut(s) 1056
BspT107I GGYRCC 2 cut(s) 626, 728
BsrDI GCAATG 2 cut(s) 272, 963
BsrFI RCCGGY 1 cut(s) 159
BssAI RCCGGY 1 cut(s) 159
BssECI CCNNGG 6 cut(s) 398, 466, 467, 691, 1042, 1362
BssMI GATC 7 cut(s) 170, 471, 1225, 1273, 1465, 1543, 1548
BssNAI GTATAC 1 cut(s) 709
BssSI CACGAG 2 cut(s) 645, 1428
BssT1I CCWWGG 1 cut(s) 1362
Bst1107I GTATAC 1 cut(s) 709
Bst2BI CACGAG 2 cut(s) 645, 1428
Bst2UI CCWGG 1 cut(s) 1043
Bst4CI ACNGT 4 cut(s) 481, 1376, 1387, 1453
Bst6I CTCTTC 2 cut(s) 39, 1338
BstAPI GCANNNNNTGC 1 cut(s) 974
BstBAI YACGTR 1 cut(s) 898
BstBI TTCGAA 1 cut(s) 1056
BstC8I GCNNGC 1 cut(s) 435
BstDEI CTNAG 2 cut(s) 174, 873
BstDSI CCRYGG 1 cut(s) 1362
BstF5I GGATG 2 cut(s) 334, 1476
BstHHI GCGC 1 cut(s) 340
BstKTI GATC 7 cut(s) 173, 474, 1228, 1276, 1468, 1546, 1551
BstMAI GTCTC 2 cut(s) 147, 1433
BstMBI GATC 7 cut(s) 170, 471, 1225, 1273, 1465, 1543, 1548
BstMWI GCNNNNNNNGC 8 cut(s) 63, 111, 878, 887, 974, 983, 1115, 1346
BstNI CCWGG 1 cut(s) 1043
BstNSI RCATGY 3 cut(s) 453, 517, 1412
BstSCI CCNGG 5 cut(s) 465, 466, 690, 1041, 1118
BstV1I GCAGC 3 cut(s) 755, 877, 1138
BstV2I GAAGAC 3 cut(s) 18, 225, 770
BstX2I RGATCY 1 cut(s) 1273
BstXI CCANNNNNNTGG 1 cut(s) 1154
BstYI RGATCY 1 cut(s) 1273
BstZ17I GTATAC 1 cut(s) 709
BsuI GTATCC 1 cut(s) 1364
BsuRI GGCC 5 cut(s) 163, 397, 1041, 1361, 1393
BtgI CCRYGG 1 cut(s) 1362
BtrI CACGTC 1 cut(s) 389
BtsCI GGATG 2 cut(s) 334, 1476
BtsI GCAGTG 1 cut(s) 855
BtsIMutI CAGTG 4 cut(s) 855, 961, 1381, 1458
Cac8I GCNNGC 1 cut(s) 435
CaiI CAGNNNCTG 1 cut(s) 794
CciI TCATGA 1 cut(s) 655
CfoI GCGC 1 cut(s) 340
Cfr10I RCCGGY 1 cut(s) 159
Cfr13I GGNCC 4 cut(s) 395, 688, 1359, 1391
Cfr9I CCCGGG 1 cut(s) 466
CseI GACGC 2 cut(s) 1061, 1450
Csp6I GTAC 1 cut(s) 377
CviAII CATG 7 cut(s) 450, 514, 656, 1148, 1355, 1363, 1409
CviQI GTAC 1 cut(s) 377
DdeI CTNAG 2 cut(s) 174, 873
DpnI GATC 7 cut(s) 172, 473, 1227, 1275, 1467, 1545, 1550
DpnII GATC 7 cut(s) 170, 471, 1225, 1273, 1465, 1543, 1548
DrdI GACNNNNNNGTC 1 cut(s) 209
DseDI GACNNNNNNGTC 1 cut(s) 209
Eam1104I CTCTTC 2 cut(s) 39, 1338
EarI CTCTTC 2 cut(s) 39, 1338
EciI GGCGGA 1 cut(s) 92
Ecl136II GAGCTC 1 cut(s) 180
Eco130I CCWWGG 1 cut(s) 1362
Eco24I GRGCYC 2 cut(s) 182, 323
Eco32I GATATC 1 cut(s) 610
Eco47I GGWCC 1 cut(s) 688
Eco53kI GAGCTC 1 cut(s) 180
Eco72I CACGTG 1 cut(s) 898
Eco88I CYCGRG 2 cut(s) 399, 466
EcoICRI GAGCTC 1 cut(s) 180
EcoO109I RGGNCCY 1 cut(s) 395
EcoRII CCWGG 1 cut(s) 1041
EcoRV GATATC 1 cut(s) 610
EcoT14I CCWWGG 1 cut(s) 1362
EcoT22I ATGCAT 2 cut(s) 1171, 1312
EcoT38I GRGCYC 2 cut(s) 182, 323
ErhI CCWWGG 1 cut(s) 1362
Esp3I CGTCTC 2 cut(s) 147, 1433
FaeI CATG 7 cut(s) 453, 517, 659, 1151, 1358, 1366, 1412
FatI CATG 7 cut(s) 449, 513, 655, 1147, 1354, 1362, 1408
FauI CCCGC 2 cut(s) 158, 612
FbaI TGATCA 1 cut(s) 1548
FblI GTMKAC 1 cut(s) 708
Fnu4HI GCNGC 5 cut(s) 55, 194, 744, 891, 1152
FokI GGATG 2 cut(s) 341, 1483
FriOI GRGCYC 2 cut(s) 182, 323
Fsp4HI GCNGC 5 cut(s) 55, 194, 744, 891, 1152
FspBI CTAG 5 cut(s) 369, 1190, 1479, 1506, 1536
FspI TGCGCA 1 cut(s) 339
GlaI GCGC 1 cut(s) 339
GluI GCNGC 5 cut(s) 55, 194, 744, 891, 1152
GsaI CCCAGC 2 cut(s) 798, 1141
GsuI CTGGAG 1 cut(s) 1176
HaeIII GGCC 5 cut(s) 163, 397, 1041, 1361, 1393
HapII CCGG 4 cut(s) 160, 467, 691, 1119
HgaI GACGC 2 cut(s) 1061, 1450
HhaI GCGC 1 cut(s) 340
Hin1II CATG 7 cut(s) 453, 517, 659, 1151, 1358, 1366, 1412
Hin6I GCGC 1 cut(s) 338
HinP1I GCGC 1 cut(s) 338
HincII GTYRAC 4 cut(s) 588, 1209, 1399, 1461
HindII GTYRAC 4 cut(s) 588, 1209, 1399, 1461
HindIII AAGCTT 2 cut(s) 78, 288
HpaI GTTAAC 2 cut(s) 1209, 1399
HpaII CCGG 4 cut(s) 160, 467, 691, 1119
HphI GGTGA 1 cut(s) 734
Hpy166II GTNNAC 5 cut(s) 588, 709, 1209, 1399, 1461
Hpy8I GTNNAC 5 cut(s) 588, 709, 1209, 1399, 1461
Hpy99I CGWCG 3 cut(s) 206, 390, 1498
HpyAV CCTTC 4 cut(s) 54, 139, 1031, 1106
HpyCH4III ACNGT 4 cut(s) 481, 1376, 1387, 1453
HpyCH4IV ACGT 4 cut(s) 222, 388, 772, 897
HpyF10VI GCNNNNNNNGC 8 cut(s) 63, 111, 878, 887, 974, 983, 1115, 1346
HpyF3I CTNAG 2 cut(s) 174, 873
HpySE526I ACGT 4 cut(s) 222, 388, 772, 897
Hsp92II CATG 7 cut(s) 453, 517, 659, 1151, 1358, 1366, 1412
HspAI GCGC 1 cut(s) 338
Ksp22I TGATCA 1 cut(s) 1548
KspAI GTTAAC 2 cut(s) 1209, 1399
Kzo9I GATC 7 cut(s) 170, 471, 1225, 1273, 1465, 1543, 1548
LmnI GCTCC 3 cut(s) 71, 740, 1115
Lsp1109I GCAGC 3 cut(s) 755, 877, 1138
LweI GCATC 5 cut(s) 319, 1156, 1178, 1319, 1499
MaeI CTAG 5 cut(s) 369, 1190, 1479, 1506, 1536
MaeII ACGT 4 cut(s) 222, 388, 772, 897
MaeIII GTNAC 3 cut(s) 95, 445, 1432
MalI GATC 7 cut(s) 172, 473, 1227, 1275, 1467, 1545, 1550
MboI GATC 7 cut(s) 170, 471, 1225, 1273, 1465, 1543, 1548
MboII GAAGA 7 cut(s) 18, 26, 230, 770, 878, 960, 1325
MfeI CAATTG 1 cut(s) 810
MflI RGATCY 1 cut(s) 1273
MhlI GDGCHC 2 cut(s) 182, 323
MluCI AATT 8 cut(s) 84, 238, 540, 714, 810, 930, 1058, 1422
MlyI GAGTC 1 cut(s) 54
MmeI TCCRAC 2 cut(s) 402, 1301
Mph1103I ATGCAT 2 cut(s) 1171, 1312
MroXI GAANNNNTTC 1 cut(s) 222
MseI TTAA 6 cut(s) 581, 885, 1077, 1208, 1221, 1398
MspI CCGG 4 cut(s) 160, 467, 691, 1119
MspR9I CCNGG 5 cut(s) 467, 468, 692, 1043, 1120
MunI CAATTG 1 cut(s) 810
MvaI CCWGG 1 cut(s) 1043
MwoI GCNNNNNNNGC 8 cut(s) 63, 111, 878, 887, 974, 983, 1115, 1346
NciI CCSGG 4 cut(s) 467, 468, 692, 1120
NcoI CCATGG 1 cut(s) 1362
NdeII GATC 7 cut(s) 170, 471, 1225, 1273, 1465, 1543, 1548
NlaIII CATG 7 cut(s) 453, 517, 659, 1151, 1358, 1366, 1412
NlaIV GGNNCC 6 cut(s) 67, 628, 730, 801, 1117, 1360
NmeAIII GCCGAG 1 cut(s) 42
NmuCI GTSAC 2 cut(s) 445, 1432
NsbI TGCGCA 1 cut(s) 339
NsiI ATGCAT 2 cut(s) 1171, 1312
NspI RCATGY 3 cut(s) 453, 517, 1412
NspV TTCGAA 1 cut(s) 1056
PagI TCATGA 1 cut(s) 655
PciI ACATGT 2 cut(s) 449, 513
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PdmI GAANNNNTTC 1 cut(s) 222
PkrI GCNGC 5 cut(s) 56, 195, 745, 892, 1153
PleI GAGTC 1 cut(s) 54
PmaCI CACGTG 1 cut(s) 898
PmlI CACGTG 1 cut(s) 898
PpsI GAGTC 1 cut(s) 54
Ppu21I YACGTR 1 cut(s) 898
PscI ACATGT 2 cut(s) 449, 513
PshBI ATTAAT 1 cut(s) 885
Psp124BI GAGCTC 1 cut(s) 182
Psp6I CCWGG 1 cut(s) 1041
PspCI CACGTG 1 cut(s) 898
PspFI CCCAGC 2 cut(s) 794, 1137
PspGI CCWGG 1 cut(s) 1041
PspN4I GGNNCC 6 cut(s) 67, 628, 730, 801, 1117, 1360
PspPI GGNCC 4 cut(s) 395, 688, 1359, 1391
PstNI CAGNNNCTG 1 cut(s) 794
PsuI RGATCY 1 cut(s) 1273
RsaI GTAC 1 cut(s) 378
RsaNI GTAC 1 cut(s) 377
SacI GAGCTC 1 cut(s) 182
SaqAI TTAA 6 cut(s) 581, 885, 1077, 1208, 1221, 1398
SatI GCNGC 5 cut(s) 55, 194, 744, 891, 1152
Sau3AI GATC 7 cut(s) 170, 471, 1225, 1273, 1465, 1543, 1548
Sau96I GGNCC 4 cut(s) 395, 688, 1359, 1391
SchI GAGTC 1 cut(s) 54
ScrFI CCNGG 5 cut(s) 467, 468, 692, 1043, 1120
SduI GDGCHC 2 cut(s) 182, 323
SfaNI GCATC 5 cut(s) 319, 1156, 1178, 1319, 1499
SfuI TTCGAA 1 cut(s) 1056
SinI GGWCC 1 cut(s) 688
SmaI CCCGGG 1 cut(s) 468
SmlI CTYRAG 1 cut(s) 505
SmoI CTYRAG 1 cut(s) 505
Sse9I AATT 8 cut(s) 84, 238, 540, 714, 810, 930, 1058, 1422
SsiI CCGC 8 cut(s) 55, 103, 151, 194, 326, 332, 619, 1253
SspMI CTAG 5 cut(s) 369, 1190, 1479, 1506, 1536
SstI GAGCTC 1 cut(s) 182
StyD4I CCNGG 5 cut(s) 465, 466, 690, 1041, 1118
StyI CCWWGG 1 cut(s) 1362
TaaI ACNGT 4 cut(s) 481, 1376, 1387, 1453
TaiI ACGT 4 cut(s) 225, 391, 775, 900
TaqI TCGA 8 cut(s) 182, 216, 385, 487, 991, 1056, 1291, 1493
TasI AATT 8 cut(s) 84, 238, 540, 714, 810, 930, 1058, 1422
TauI GCSGC 2 cut(s) 57, 196
Tru1I TTAA 6 cut(s) 581, 885, 1077, 1208, 1221, 1398
Tru9I TTAA 6 cut(s) 581, 885, 1077, 1208, 1221, 1398
TscAI CASTG 4 cut(s) 862, 961, 1381, 1458
TseFI GTSAC 2 cut(s) 445, 1432
TseI GCWGC 3 cut(s) 743, 890, 1151
Tsp45I GTSAC 2 cut(s) 445, 1432
TspDTI ATGAA 3 cut(s) 952, 1277, 1343
TspGWI ACGGA 1 cut(s) 129
TspMI CCCGGG 1 cut(s) 466
TspRI CASTG 4 cut(s) 862, 961, 1381, 1458
VpaK11BI GGWCC 1 cut(s) 688
VspI ATTAAT 1 cut(s) 885
XapI RAATTY 1 cut(s) 1058
XbaI TCTAGA 1 cut(s) 1535
XceI RCATGY 3 cut(s) 453, 517, 1412
XcmI CCANNNNNNNNNTGG 1 cut(s) 620
XmaI CCCGGG 1 cut(s) 466
XmiI GTMKAC 1 cut(s) 708
XmnI GAANNNNTTC 1 cut(s) 222
XspI CTAG 5 cut(s) 369, 1190, 1479, 1506, 1536
Zsp2I ATGCAT 2 cut(s) 1171, 1312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.