Rroxscaffold_4G00284080

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0008946
Physical Location & Seq
Forward (+)
5803778 .. 5808736
4959 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00284080.1

Sequence Viewer

Length: 1416 bp
ATGGATTCCACCATTTCGTTATCGTCTTCCTCTCTTCTCCTACCTTCGCCTCGCCGCCGACTCGGCTCCAAATCCCAAGCTTCAATTTCGCTCCGTAACCTCCGCCGTCCGAGCCTCAAATCCAGAACCTTCCTCGTTCCGTCTCTTTCCCGCCAAAACCGGCCTATCCGATCTCAGAGCTCGAATCATCAGCCGCAGGACGACGGCTTCGTCCTCGAAGACGTTCCTCACTTGACCGATTTCCTCCCAAACTTGCCGTCATATCCGAATCCATTGCAACAGAGCCAAGCTTATGCTGTTGTCAAGAAAACTTTTGTGAGCCCTGCGGATGCAGTTGCGCAGAAAGTTGTTGTTCAAGATGATAGTCCTAGAGGGGTACATTTTCGACGTGCAGGGCCTCGGGAAAAGGTTTATTTCAAGTCGGAGGAAGTGCGTGCTTGTATAGTGACATGTGGGGGTTTATGCCCGGGGATCAATACTGTGATTCGAGAGATAGTATGTGGCTTGAACTACATGTATGGTGTTGAGGATATACTTGGAATTGAGGGAGGATATAGAGGCTTTTATTCTAAAAATACATTAAAGTTGACACCTAAAGTTGTCAATGATATCCATAAGCGGGGTGGCACCTTTCTCCGCACTTCACGAGGAGGTCATGATACCAACAAGATAGTTGATAACATACAGGACCGGGGAATAAATCAGGAAGTTGAGAAACGTGGTCTTCAAGTGGCAGTTGCTGGGGTTCCTAAAACAATTGACAATGATATTGCTGTGATAGACAAGTCTTTTGGTTTTGACACTGCTGTTGAAGAAGCTCAAAGGGCTATTAATGCTGCACACGTGGAGGTTGAAGGTGTGGAAAATGGAATCGGAATTGTCAAACTGATGGGAAGATACAGTGAATCTCCATTCTATTTGGAAGGCCAGGGTGGGCTTTTCGAATTTATTGAACAGCATCTTAAAGAGAATGGACATATAGTTATCGTAGTAGCAGAAGGAGCCGGGCAGGAGTATGTTGCCCAGCAAATCCATGCTGCTGGAGAGAAAGATGCATCTGGAAATAAGTTGCTACTAGATTGCTCCTACAATGCTTTTCTATCTGTAGATCCAACATATATGATTCGAGCTATTCCAAGTAATGCATCAGACAATATATACTGCACTCTTCTGGCTCATAGTGCTGTTCATGGGGCCATGGCTGGATACAGTGGTTTCACGGTTGGGCCTGTTAACAGCAGACATGCCTATATTCCAATTTCTCGTGTGACAGAGAAGCAAAACACAGTGAACTTGACAGATCGGATGTGGGCTAGACTTCTCGCATCGACGAATCAGCCTAGTTTTTTGGAGAGAAATGAGTTGCAGTCTAGAGTTGATCTGATCAACAGCAACTTGGAAAATACATCTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003872 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008443 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009536 GO:0009987 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0061615 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230
Pfam Domains
Protein Families
PFK

Protein Analysis

471

Amino Acids

51.74

Weight (kDa)

8.12

Isoelectric Point (pI)

52.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PFK PF00365 146 - 301 3.2e-30 Phosphofructokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 209
Acc16I TGCGCA 1 cut(s) 339
AccB1I GGYRCC 1 cut(s) 626
AciI CCGC 7 cut(s) 55, 103, 151, 194, 326, 619, 637
AclWI GGATC 2 cut(s) 479, 1103
AcsI RAATTY 1 cut(s) 944
AcvI CACGTG 1 cut(s) 844
AfaI GTAC 1 cut(s) 378
AfiI CCNNNNNNNGG 1 cut(s) 619
AflIII ACRYGT 3 cut(s) 449, 513, 841
AgsI TTSAA 8 cut(s) 84, 356, 418, 508, 728, 812, 854, 953
AjiI CACGTC 1 cut(s) 389
AjnI CCWGG 1 cut(s) 927
AluBI AGCT 5 cut(s) 80, 180, 290, 818, 1130
AluI AGCT 5 cut(s) 80, 180, 290, 818, 1130
Alw21I GWGCWC 1 cut(s) 182
Alw26I GTCTC 1 cut(s) 147
AlwI GGATC 2 cut(s) 479, 1103
AlwNI CAGNNNCTG 1 cut(s) 740
Ama87I CYCGRG 2 cut(s) 399, 466
AoxI GGCC 5 cut(s) 161, 395, 925, 1194, 1226
ApeKI GCWGC 2 cut(s) 836, 1037
ApoI RAATTY 1 cut(s) 944
ArsI GACNNNNNNTTYG 6 cut(s) 191, 223, 242, 274, 773, 805
AseI ATTAAT 1 cut(s) 831
Asp700I GAANNNNTTC 1 cut(s) 222
AspLEI GCGC 1 cut(s) 340
AspS9I GGNCC 4 cut(s) 395, 688, 1194, 1226
AsuC2I CCSGG 4 cut(s) 467, 468, 692, 1006
AsuII TTCGAA 1 cut(s) 942
AvaI CYCGRG 2 cut(s) 399, 466
AvaII GGWCC 1 cut(s) 688
BaeI ACNNNNGTAYC 2 cut(s) 1198, 1231
BanI GGYRCC 1 cut(s) 626
BanII GRGCYC 2 cut(s) 182, 323
BauI CACGAG 2 cut(s) 645, 1263
BbrPI CACGTG 1 cut(s) 844
BbsI GAAGAC 3 cut(s) 18, 225, 716
Bbv12I GWGCWC 1 cut(s) 182
BbvI GCAGC 2 cut(s) 823, 1024
BccI CCATC 1 cut(s) 883
BceAI ACGGC 3 cut(s) 90, 220, 241
BcgI CGANNNNNNTGC 2 cut(s) 256, 290
BciT130I CCWGG 1 cut(s) 929
BciVI GTATCC 1 cut(s) 1199
BclI TGATCA 1 cut(s) 1383
BcnI CCSGG 4 cut(s) 467, 468, 692, 1006
BcoDI GTCTC 1 cut(s) 147
BfaI CTAG 5 cut(s) 369, 1076, 1314, 1341, 1371
BfmI CTRYAG 1 cut(s) 1104
BfuI GTATCC 1 cut(s) 1199
BglI GCCNNNNNGGC 1 cut(s) 63
BisI GCNGC 4 cut(s) 55, 194, 837, 1038
BlsI GCNGC 4 cut(s) 56, 195, 838, 1039
Bme1390I CCNGG 5 cut(s) 467, 468, 692, 929, 1006
Bme18I GGWCC 1 cut(s) 688
BmeT110I CYCGRG 2 cut(s) 399, 466
BmgBI CACGTC 1 cut(s) 389
BmgT120I GGNCC 4 cut(s) 395, 688, 1194, 1226
BmiI GGNNCC 5 cut(s) 67, 628, 747, 1003, 1195
BmrFI CCNGG 5 cut(s) 467, 468, 692, 929, 1006
BmsI GCATC 6 cut(s) 319, 967, 1042, 1064, 1154, 1334
BpiI GAAGAC 3 cut(s) 18, 225, 716
BpmI CTGGAG 1 cut(s) 1062
Bpu14I TTCGAA 1 cut(s) 942
BpuMI CCSGG 4 cut(s) 467, 468, 692, 1006
BsaAI YACGTR 1 cut(s) 844
BsaJI CCNNGG 6 cut(s) 398, 466, 467, 691, 928, 1197
Bsc4I CCNNNNNNNGG 1 cut(s) 619
Bse118I RCCGGY 1 cut(s) 159
Bse3DI GCAATG 1 cut(s) 272
BseBI CCWGG 1 cut(s) 929
BseDI CCNNGG 6 cut(s) 398, 466, 467, 691, 928, 1197
BseGI GGATG 2 cut(s) 334, 1311
BseLI CCNNNNNNNGG 1 cut(s) 619
BseMI GCAATG 1 cut(s) 272
BseMII CTCAG 1 cut(s) 188
BseRI GAGGAG 1 cut(s) 663
BseXI GCAGC 2 cut(s) 823, 1024
BseYI CCCAGC 2 cut(s) 740, 1023
BsgI GTGCAG 3 cut(s) 411, 822, 1147
BshFI GGCC 5 cut(s) 163, 397, 927, 1196, 1228
BshNI GGYRCC 1 cut(s) 626
BsiHKAI GWGCWC 1 cut(s) 182
BsiHKCI CYCGRG 2 cut(s) 399, 466
BsiSI CCGG 4 cut(s) 160, 467, 691, 1005
BslI CCNNNNNNNGG 1 cut(s) 619
BsmAI GTCTC 1 cut(s) 147
BsmBI CGTCTC 1 cut(s) 147
BsnI GGCC 5 cut(s) 163, 397, 927, 1196, 1228
BsoBI CYCGRG 2 cut(s) 399, 466
Bsp119I TTCGAA 1 cut(s) 942
Bsp1286I GDGCHC 2 cut(s) 182, 323
Bsp143I GATC 6 cut(s) 170, 471, 1108, 1300, 1378, 1383
Bsp19I CCATGG 1 cut(s) 1197
BspACI CCGC 7 cut(s) 55, 103, 151, 194, 326, 619, 637
BspANI GGCC 5 cut(s) 163, 397, 927, 1196, 1228
BspCNI CTCAG 1 cut(s) 187
BspHI TCATGA 1 cut(s) 655
BspLI GGNNCC 5 cut(s) 67, 628, 747, 1003, 1195
BspPI GGATC 2 cut(s) 479, 1103
BspT104I TTCGAA 1 cut(s) 942
BspT107I GGYRCC 1 cut(s) 626
BsrDI GCAATG 1 cut(s) 272
BsrFI RCCGGY 1 cut(s) 159
BssAI RCCGGY 1 cut(s) 159
BssECI CCNNGG 6 cut(s) 398, 466, 467, 691, 928, 1197
BssMI GATC 6 cut(s) 170, 471, 1108, 1300, 1378, 1383
BssSI CACGAG 2 cut(s) 645, 1263
BssT1I CCWWGG 1 cut(s) 1197
Bst2BI CACGAG 2 cut(s) 645, 1263
Bst2UI CCWGG 1 cut(s) 929
Bst4CI ACNGT 5 cut(s) 481, 902, 1211, 1222, 1288
Bst6I CTCTTC 2 cut(s) 39, 1173
BstBAI YACGTR 1 cut(s) 844
BstBI TTCGAA 1 cut(s) 942
BstC8I GCNNGC 1 cut(s) 435
BstDEI CTNAG 1 cut(s) 174
BstDSI CCRYGG 1 cut(s) 1197
BstF5I GGATG 2 cut(s) 334, 1311
BstHHI GCGC 1 cut(s) 340
BstKTI GATC 6 cut(s) 173, 474, 1111, 1303, 1381, 1386
BstMAI GTCTC 1 cut(s) 147
BstMBI GATC 6 cut(s) 170, 471, 1108, 1300, 1378, 1383
BstMWI GCNNNNNNNGC 6 cut(s) 63, 111, 824, 833, 1001, 1181
BstNI CCWGG 1 cut(s) 929
BstNSI RCATGY 3 cut(s) 453, 517, 1247
BstSCI CCNGG 5 cut(s) 465, 466, 690, 927, 1004
BstSFI CTRYAG 1 cut(s) 1104
BstV1I GCAGC 2 cut(s) 823, 1024
BstV2I GAAGAC 3 cut(s) 18, 225, 716
BstX2I RGATCY 1 cut(s) 1108
BstXI CCANNNNNNTGG 1 cut(s) 1040
BstYI RGATCY 1 cut(s) 1108
BsuI GTATCC 1 cut(s) 1199
BsuRI GGCC 5 cut(s) 163, 397, 927, 1196, 1228
BtgI CCRYGG 1 cut(s) 1197
BtrI CACGTC 1 cut(s) 389
BtsCI GGATG 2 cut(s) 334, 1311
BtsI GCAGTG 1 cut(s) 801
BtsIMutI CAGTG 4 cut(s) 801, 907, 1216, 1293
Cac8I GCNNGC 1 cut(s) 435
CaiI CAGNNNCTG 1 cut(s) 740
CciI TCATGA 1 cut(s) 655
CfoI GCGC 1 cut(s) 340
Cfr10I RCCGGY 1 cut(s) 159
Cfr13I GGNCC 4 cut(s) 395, 688, 1194, 1226
Cfr9I CCCGGG 1 cut(s) 466
Csp6I GTAC 1 cut(s) 377
CviAII CATG 7 cut(s) 450, 514, 656, 1034, 1190, 1198, 1244
CviQI GTAC 1 cut(s) 377
DdeI CTNAG 1 cut(s) 174
DpnI GATC 6 cut(s) 172, 473, 1110, 1302, 1380, 1385
DpnII GATC 6 cut(s) 170, 471, 1108, 1300, 1378, 1383
DrdI GACNNNNNNGTC 1 cut(s) 209
DseDI GACNNNNNNGTC 1 cut(s) 209
Eam1104I CTCTTC 2 cut(s) 39, 1173
EarI CTCTTC 2 cut(s) 39, 1173
EciI GGCGGA 1 cut(s) 92
Ecl136II GAGCTC 1 cut(s) 180
Eco130I CCWWGG 1 cut(s) 1197
Eco24I GRGCYC 2 cut(s) 182, 323
Eco32I GATATC 1 cut(s) 610
Eco47I GGWCC 1 cut(s) 688
Eco53kI GAGCTC 1 cut(s) 180
Eco72I CACGTG 1 cut(s) 844
Eco88I CYCGRG 2 cut(s) 399, 466
EcoICRI GAGCTC 1 cut(s) 180
EcoO109I RGGNCCY 1 cut(s) 395
EcoRII CCWGG 1 cut(s) 927
EcoRV GATATC 1 cut(s) 610
EcoT14I CCWWGG 1 cut(s) 1197
EcoT22I ATGCAT 2 cut(s) 1057, 1147
EcoT38I GRGCYC 2 cut(s) 182, 323
ErhI CCWWGG 1 cut(s) 1197
Esp3I CGTCTC 1 cut(s) 147
FaeI CATG 7 cut(s) 453, 517, 659, 1037, 1193, 1201, 1247
FatI CATG 7 cut(s) 449, 513, 655, 1033, 1189, 1197, 1243
FauI CCCGC 2 cut(s) 158, 612
FbaI TGATCA 1 cut(s) 1383
Fnu4HI GCNGC 4 cut(s) 55, 194, 837, 1038
FokI GGATG 2 cut(s) 341, 1318
FriOI GRGCYC 2 cut(s) 182, 323
Fsp4HI GCNGC 4 cut(s) 55, 194, 837, 1038
FspBI CTAG 5 cut(s) 369, 1076, 1314, 1341, 1371
FspI TGCGCA 1 cut(s) 339
GlaI GCGC 1 cut(s) 339
GluI GCNGC 4 cut(s) 55, 194, 837, 1038
GsaI CCCAGC 2 cut(s) 744, 1027
GsuI CTGGAG 1 cut(s) 1062
HaeIII GGCC 5 cut(s) 163, 397, 927, 1196, 1228
HapII CCGG 4 cut(s) 160, 467, 691, 1005
HhaI GCGC 1 cut(s) 340
Hin1II CATG 7 cut(s) 453, 517, 659, 1037, 1193, 1201, 1247
Hin6I GCGC 1 cut(s) 338
HinP1I GCGC 1 cut(s) 338
HincII GTYRAC 2 cut(s) 588, 1234
HindII GTYRAC 2 cut(s) 588, 1234
HindIII AAGCTT 2 cut(s) 78, 288
HinfI GANTC 9 cut(s) 5, 60, 184, 268, 484, 870, 905, 1123, 1333
HpaI GTTAAC 1 cut(s) 1234
HpaII CCGG 4 cut(s) 160, 467, 691, 1005
Hpy166II GTNNAC 3 cut(s) 588, 1234, 1291
Hpy188I TCNGA 9 cut(s) 111, 170, 177, 267, 424, 875, 1150, 1305, 1383
Hpy8I GTNNAC 3 cut(s) 588, 1234, 1291
Hpy99I CGWCG 3 cut(s) 206, 390, 1333
HpyAV CCTTC 5 cut(s) 54, 139, 848, 917, 992
HpyCH4III ACNGT 5 cut(s) 481, 902, 1211, 1222, 1288
HpyCH4IV ACGT 4 cut(s) 222, 388, 718, 843
HpyCH4V TGCA 8 cut(s) 277, 332, 392, 839, 1055, 1145, 1164, 1366
HpyF10VI GCNNNNNNNGC 6 cut(s) 63, 111, 824, 833, 1001, 1181
HpyF3I CTNAG 1 cut(s) 174
HpySE526I ACGT 4 cut(s) 222, 388, 718, 843
Hsp92II CATG 7 cut(s) 453, 517, 659, 1037, 1193, 1201, 1247
HspAI GCGC 1 cut(s) 338
Ksp22I TGATCA 1 cut(s) 1383
KspAI GTTAAC 1 cut(s) 1234
Kzo9I GATC 6 cut(s) 170, 471, 1108, 1300, 1378, 1383
LmnI GCTCC 4 cut(s) 71, 96, 1001, 1088
Lsp1109I GCAGC 2 cut(s) 823, 1024
LweI GCATC 6 cut(s) 319, 967, 1042, 1064, 1154, 1334
MaeI CTAG 5 cut(s) 369, 1076, 1314, 1341, 1371
MaeII ACGT 4 cut(s) 222, 388, 718, 843
MaeIII GTNAC 3 cut(s) 95, 445, 1267
MalI GATC 6 cut(s) 172, 473, 1110, 1302, 1380, 1385
MboI GATC 6 cut(s) 170, 471, 1108, 1300, 1378, 1383
MboII GAAGA 7 cut(s) 18, 26, 230, 716, 824, 906, 1160
MfeI CAATTG 1 cut(s) 756
MflI RGATCY 1 cut(s) 1108
MhlI GDGCHC 2 cut(s) 182, 323
MluCI AATT 6 cut(s) 84, 540, 756, 876, 944, 1257
MlyI GAGTC 1 cut(s) 54
MmeI TCCRAC 2 cut(s) 402, 1136
Mph1103I ATGCAT 2 cut(s) 1057, 1147
MroXI GAANNNNTTC 1 cut(s) 222
MseI TTAA 4 cut(s) 581, 831, 963, 1233
MspI CCGG 4 cut(s) 160, 467, 691, 1005
MspR9I CCNGG 5 cut(s) 467, 468, 692, 929, 1006
MunI CAATTG 1 cut(s) 756
MvaI CCWGG 1 cut(s) 929
MwoI GCNNNNNNNGC 6 cut(s) 63, 111, 824, 833, 1001, 1181
NciI CCSGG 4 cut(s) 467, 468, 692, 1006
NcoI CCATGG 1 cut(s) 1197
NdeII GATC 6 cut(s) 170, 471, 1108, 1300, 1378, 1383
NlaIII CATG 7 cut(s) 453, 517, 659, 1037, 1193, 1201, 1247
NlaIV GGNNCC 5 cut(s) 67, 628, 747, 1003, 1195
NmeAIII GCCGAG 1 cut(s) 42
NmuCI GTSAC 2 cut(s) 445, 1267
NsbI TGCGCA 1 cut(s) 339
NsiI ATGCAT 2 cut(s) 1057, 1147
NspI RCATGY 3 cut(s) 453, 517, 1247
NspV TTCGAA 1 cut(s) 942
PagI TCATGA 1 cut(s) 655
PciI ACATGT 2 cut(s) 449, 513
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PdmI GAANNNNTTC 1 cut(s) 222
PfeI GAWTC 8 cut(s) 5, 184, 268, 484, 870, 905, 1123, 1333
PkrI GCNGC 4 cut(s) 56, 195, 838, 1039
PleI GAGTC 1 cut(s) 54
PmaCI CACGTG 1 cut(s) 844
PmlI CACGTG 1 cut(s) 844
PpsI GAGTC 1 cut(s) 54
Ppu21I YACGTR 1 cut(s) 844
PscI ACATGT 2 cut(s) 449, 513
PshBI ATTAAT 1 cut(s) 831
Psp124BI GAGCTC 1 cut(s) 182
Psp6I CCWGG 1 cut(s) 927
PspCI CACGTG 1 cut(s) 844
PspFI CCCAGC 2 cut(s) 740, 1023
PspGI CCWGG 1 cut(s) 927
PspN4I GGNNCC 5 cut(s) 67, 628, 747, 1003, 1195
PspPI GGNCC 4 cut(s) 395, 688, 1194, 1226
PstNI CAGNNNCTG 1 cut(s) 740
PsuI RGATCY 1 cut(s) 1108
RsaI GTAC 1 cut(s) 378
RsaNI GTAC 1 cut(s) 377
SacI GAGCTC 1 cut(s) 182
SaqAI TTAA 4 cut(s) 581, 831, 963, 1233
SatI GCNGC 4 cut(s) 55, 194, 837, 1038
Sau3AI GATC 6 cut(s) 170, 471, 1108, 1300, 1378, 1383
Sau96I GGNCC 4 cut(s) 395, 688, 1194, 1226
SchI GAGTC 1 cut(s) 54
ScrFI CCNGG 5 cut(s) 467, 468, 692, 929, 1006
SduI GDGCHC 2 cut(s) 182, 323
SfaNI GCATC 6 cut(s) 319, 967, 1042, 1064, 1154, 1334
SfcI CTRYAG 1 cut(s) 1104
SfuI TTCGAA 1 cut(s) 942
SinI GGWCC 1 cut(s) 688
SmaI CCCGGG 1 cut(s) 468
Sse9I AATT 6 cut(s) 84, 540, 756, 876, 944, 1257
SsiI CCGC 7 cut(s) 55, 103, 151, 194, 326, 619, 637
SspMI CTAG 5 cut(s) 369, 1076, 1314, 1341, 1371
SstI GAGCTC 1 cut(s) 182
StyD4I CCNGG 5 cut(s) 465, 466, 690, 927, 1004
StyI CCWWGG 1 cut(s) 1197
TaaI ACNGT 5 cut(s) 481, 902, 1211, 1222, 1288
TaiI ACGT 4 cut(s) 225, 391, 721, 846
TaqI TCGA 7 cut(s) 182, 216, 385, 487, 942, 1126, 1328
TaqII GACCGA 1 cut(s) 251
TasI AATT 6 cut(s) 84, 540, 756, 876, 944, 1257
TauI GCSGC 2 cut(s) 57, 196
TfiI GAWTC 8 cut(s) 5, 184, 268, 484, 870, 905, 1123, 1333
Tru1I TTAA 4 cut(s) 581, 831, 963, 1233
Tru9I TTAA 4 cut(s) 581, 831, 963, 1233
TscAI CASTG 4 cut(s) 808, 907, 1216, 1293
TseFI GTSAC 2 cut(s) 445, 1267
TseI GCWGC 2 cut(s) 836, 1037
Tsp45I GTSAC 2 cut(s) 445, 1267
TspDTI ATGAA 1 cut(s) 1178
TspGWI ACGGA 2 cut(s) 83, 129
TspMI CCCGGG 1 cut(s) 466
TspRI CASTG 4 cut(s) 808, 907, 1216, 1293
VpaK11BI GGWCC 1 cut(s) 688
VspI ATTAAT 1 cut(s) 831
XapI RAATTY 1 cut(s) 944
XbaI TCTAGA 1 cut(s) 1370
XceI RCATGY 3 cut(s) 453, 517, 1247
XcmI CCANNNNNNNNNTGG 1 cut(s) 620
XmaI CCCGGG 1 cut(s) 466
XmnI GAANNNNTTC 1 cut(s) 222
XspI CTAG 5 cut(s) 369, 1076, 1314, 1341, 1371
Zsp2I ATGCAT 2 cut(s) 1057, 1147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.