Prupe.2G272600_v2.0.a1

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0008946
Physical Location & Seq
Reverse (-)
27574029 .. 27579251
5223 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G272600.1

Sequence Viewer

Length: 1611 bp
ATGGAATCGGCCATTTCGTCTCCGCTTTCCACGTCGTCTCATTTGCTACCTTCGCCTTCCCGTTCATCGCTTGGCTCCAGATCTCAATCTTTCCCGTCACTTCAAAACCTCCGGCATCCGAGTTTCAAATCCAGATGCTTCCTCGGAGTTCCGTTCCGTTCGCGCCAAACCCAGCCAATCCGATGTCAAAGTTCGAATCAACAACCAGCTAAGGACGACGGTTTTGTCATCGAAGACGTTCCTCACTTGACTGATTTTCTCTCTAATTTACCGTCGTATCCAAATCCATTGAAACAGAGCCAAGCTTATGCTGTTGTCAAGCAAACTTTTGTAAGCACTGACGATGTGGTTGCGCAGAAAGTTGTTGTTCAAAAGGATGATCCCAGAGGGGTACATTTTCGACGTGCAGGACCTCGGGAAAAGGTTTATTTCAAGTCAGATGAAGTGCGTGCTTGCATAGTGACATGTGGGGGTTTATGCCCAGGGATCAATACTGTGATTCGAGAAATTGTATGTGGCTTGAACTACATGTATGGTGTGGAAGATATACTTGGAATTGAGGGAGGATATAGAGGCTTTTATTCTAAAAATACATTGAGGTTGACACCAAAAGTTGTCAATGATATCCACAAGCGTGGTGGGACCTTTCTTCGAACTTCACGAGGAGGTCATGATACAAACAAGATAGTTGATAACATACAGGACCGAGGAATAAATCAGGTGTACATAATTGGAGGTGATGGCACCCAAAAAGGAGCAGCTCTAATTTACAAGGAAGTTGAGAAACGTGGTCTTCAAGTGGCAGTTGCTGGGATCCCTAAGACAATTGATAATGATATTGCTGTGATAGACAAATCCTTTGGTTTTGATACTGCTGTTGAAGAAGCTCAGAGAGCTATTAATGCTGCACATGTGGAGGTTGAAAGTGTGGAAAATGGAGTTGGAATTGTCAAACTCATGGGCAGATACAGTGGTTTCATTGCTTTATACGCAACTTTGGCAAGTCGAGATGTGGATTGTTGCTTGGTTCCAGAATCTCCATTCTATTTGGAAGGTCGGGGTGGGCTTTTTGAATTTGTTGAAGAGCGGCTTAAAGAAAATGGACATGTGGTTATTGTGTTAGCAGAAGGAGCAGGGCAGGAATATGTTGCACAGGAAATGCAAGTGGTTGATGGTAAAGATGCATCAGGAAATAAGCTACTCCTAGATGTTGGCCTATGGCTGACTGAAAAGATTAAGGATCATTTTACAAAAGTTCAGAAGATGGCAATAAATACGAAATATATAGATCCAACATATATGATTCGTGCGATCCCAAGTAATGCATCAGACAATATATACTGCACTCTTCTTGCTCAGAGTGCTGTTCATGGGGCCATGGCAGGATTCAGTGGTTTCACAGTTGGGCCTGTAAACAGTAGACATGCTTATATTCCAATTTCTCGTGTTACAGAGACGCAAAACACAGTGAACTTGACAGATCGGATGTGGGCTAGACTTCTTGCATCGACGAACCAGCCTAGTTTCTTTGAAAGCAATGAGCTGCTTGAAAGAGTTGATAAAGAGGATGTTGATGTGATCAACCACAACATGAGAGCTACTTCCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003872 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008443 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009536 GO:0009987 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0061615 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230
Pfam Domains
Protein Families
PFK

Protein Analysis

537

Amino Acids

59.05

Weight (kDa)

6.87

Isoelectric Point (pI)

43.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 224
Acc16I TGCGCA 1 cut(s) 354
AccB1I GGYRCC 1 cut(s) 743
AccBSI CCGCTC 1 cut(s) 1087
AccI GTMKAC 1 cut(s) 1420
AccII CGCG 1 cut(s) 163
AciI CCGC 2 cut(s) 23, 1087
AclWI GGATC 7 cut(s) 374, 494, 808, 821, 1248, 1283, 1306
AcoI YGGCCR 1 cut(s) 9
AcsI RAATTY 1 cut(s) 1073
AfaI GTAC 2 cut(s) 393, 725
AflIII ACRYGT 4 cut(s) 464, 528, 910, 1105
AjiI CACGTC 2 cut(s) 33, 404
AjnI CCWGG 1 cut(s) 481
AjuI GAANNNNNNNTTGG 4 cut(s) 534, 566, 924, 956
AleI CACNNNNGTG 1 cut(s) 633
AluBI AGCT 8 cut(s) 209, 305, 761, 887, 896, 1198, 1543, 1598
AluI AGCT 8 cut(s) 209, 305, 761, 887, 896, 1198, 1543, 1598
Alw26I GTCTC 3 cut(s) 24, 42, 1448
AlwI GGATC 7 cut(s) 374, 494, 808, 821, 1248, 1283, 1306
AlwNI CAGNNNCTG 1 cut(s) 809
Ama87I CYCGRG 1 cut(s) 414
AoxI GGCC 4 cut(s) 9, 1213, 1374, 1406
ApeKI GCWGC 3 cut(s) 758, 905, 1543
ApoI RAATTY 1 cut(s) 1073
ArsI GACNNNNNNTTYG 4 cut(s) 206, 238, 842, 874
AseI ATTAAT 1 cut(s) 900
Asp700I GAANNNNTTC 1 cut(s) 237
AspLEI GCGC 2 cut(s) 165, 355
AspS9I GGNCC 5 cut(s) 410, 642, 703, 1374, 1406
AsuHPI GGTGA 1 cut(s) 749
AsuII TTCGAA 2 cut(s) 194, 652
AvaI CYCGRG 1 cut(s) 414
AvaII GGWCC 3 cut(s) 410, 642, 703
BaeI ACNNNNGTAYC 6 cut(s) 260, 293, 861, 894, 958, 991
BamHI GGATCC 1 cut(s) 813
BanI GGYRCC 1 cut(s) 743
BauI CACGAG 2 cut(s) 660, 1443
BbsI GAAGAC 2 cut(s) 240, 785
BbvI GCAGC 3 cut(s) 770, 892, 1530
BccI CCATC 3 cut(s) 734, 1166, 1258
BcgI CGANNNNNNTGC 2 cut(s) 332, 366
BciT130I CCWGG 1 cut(s) 483
BciVI GTATCC 1 cut(s) 288
BclI TGATCA 1 cut(s) 1578
BcoDI GTCTC 3 cut(s) 24, 42, 1448
BfaI CTAG 3 cut(s) 1205, 1494, 1521
BfuI GTATCC 1 cut(s) 288
BglII AGATCT 1 cut(s) 80
BisI GCNGC 4 cut(s) 759, 906, 1088, 1544
BlsI GCNGC 4 cut(s) 760, 907, 1089, 1545
Bme1390I CCNGG 1 cut(s) 483
Bme18I GGWCC 3 cut(s) 410, 642, 703
BmeT110I CYCGRG 1 cut(s) 414
BmgBI CACGTC 2 cut(s) 33, 404
BmgT120I GGNCC 5 cut(s) 410, 642, 703, 1374, 1406
BmiI GGNNCC 6 cut(s) 76, 643, 745, 815, 1029, 1375
BmrFI CCNGG 1 cut(s) 483
BmsI GCATC 6 cut(s) 124, 125, 1171, 1193, 1334, 1514
BpiI GAAGAC 2 cut(s) 240, 785
BpmI CTGGAG 1 cut(s) 61
Bpu10I CCTNAGC 1 cut(s) 210
Bpu14I TTCGAA 2 cut(s) 194, 652
BsaBI GATNNNNATC 1 cut(s) 85
BsaJI CCNNGG 6 cut(s) 142, 413, 481, 482, 706, 1377
Bse3DI GCAATG 2 cut(s) 978, 1543
Bse8I GATNNNNATC 1 cut(s) 85
BseBI CCWGG 1 cut(s) 483
BseDI CCNNGG 6 cut(s) 142, 413, 481, 482, 706, 1377
BseGI GGATG 4 cut(s) 115, 382, 1491, 1573
BseJI GATNNNNATC 1 cut(s) 85
BseMI GCAATG 2 cut(s) 978, 1543
BseMII CTCAG 2 cut(s) 902, 1370
BseRI GAGGAG 1 cut(s) 678
BseXI GCAGC 3 cut(s) 770, 892, 1530
BseYI CCCAGC 2 cut(s) 171, 809
BsgI GTGCAG 3 cut(s) 426, 891, 1327
Bsh1236I CGCG 1 cut(s) 163
BshFI GGCC 4 cut(s) 11, 1215, 1376, 1408
BshNI GGYRCC 1 cut(s) 743
BsiHKCI CYCGRG 1 cut(s) 414
BsiSI CCGG 1 cut(s) 112
BslFI GGGAC 1 cut(s) 655
BsmAI GTCTC 3 cut(s) 24, 42, 1448
BsmBI CGTCTC 3 cut(s) 24, 42, 1448
BsmFI GGGAC 1 cut(s) 655
BsnI GGCC 4 cut(s) 11, 1215, 1376, 1408
BsoBI CYCGRG 1 cut(s) 414
Bsp119I TTCGAA 2 cut(s) 194, 652
Bsp1407I TGTACA 1 cut(s) 723
Bsp143I GATC 9 cut(s) 80, 379, 486, 813, 1240, 1288, 1311, 1480, 1578
Bsp19I CCATGG 1 cut(s) 1377
BspACI CCGC 2 cut(s) 23, 1087
BspANI GGCC 4 cut(s) 11, 1215, 1376, 1408
BspCNI CTCAG 2 cut(s) 901, 1369
BspFNI CGCG 1 cut(s) 163
BspHI TCATGA 1 cut(s) 670
BspLI GGNNCC 6 cut(s) 76, 643, 745, 815, 1029, 1375
BspPI GGATC 7 cut(s) 374, 494, 808, 821, 1248, 1283, 1306
BspQI GCTCTTC 1 cut(s) 1077
BspT104I TTCGAA 2 cut(s) 194, 652
BspT107I GGYRCC 1 cut(s) 743
BsrBI CCGCTC 1 cut(s) 1087
BsrDI GCAATG 2 cut(s) 978, 1543
BsrGI TGTACA 1 cut(s) 723
BssECI CCNNGG 6 cut(s) 142, 413, 481, 482, 706, 1377
BssMI GATC 9 cut(s) 80, 379, 486, 813, 1240, 1288, 1311, 1480, 1578
BssSI CACGAG 2 cut(s) 660, 1443
BssT1I CCWWGG 1 cut(s) 1377
Bst2BI CACGAG 2 cut(s) 660, 1443
Bst2UI CCWGG 1 cut(s) 483
Bst4CI ACNGT 7 cut(s) 221, 273, 496, 971, 1402, 1418, 1468
Bst6I CTCTTC 2 cut(s) 1077, 1353
BstAUI TGTACA 1 cut(s) 723
BstBI TTCGAA 2 cut(s) 194, 652
BstC8I GCNNGC 2 cut(s) 450, 454
BstDEI CTNAG 4 cut(s) 210, 819, 888, 1356
BstDSI CCRYGG 1 cut(s) 1377
BstF5I GGATG 4 cut(s) 115, 382, 1491, 1573
BstFNI CGCG 1 cut(s) 163
BstHHI GCGC 2 cut(s) 165, 355
BstKTI GATC 9 cut(s) 83, 382, 489, 816, 1243, 1291, 1314, 1483, 1581
BstMAI GTCTC 3 cut(s) 24, 42, 1448
BstMBI GATC 9 cut(s) 80, 379, 486, 813, 1240, 1288, 1311, 1480, 1578
BstMWI GCNNNNNNNGC 7 cut(s) 52, 893, 902, 989, 998, 1130, 1361
BstNI CCWGG 1 cut(s) 483
BstNSI RCATGY 5 cut(s) 468, 532, 914, 1109, 1427
BstSCI CCNGG 1 cut(s) 481
BstUI CGCG 1 cut(s) 163
BstV1I GCAGC 3 cut(s) 770, 892, 1530
BstV2I GAAGAC 2 cut(s) 240, 785
BstX2I RGATCY 3 cut(s) 80, 813, 1288
BstXI CCANNNNNNTGG 1 cut(s) 635
BstYI RGATCY 3 cut(s) 80, 813, 1288
BsuI GTATCC 1 cut(s) 288
BsuRI GGCC 4 cut(s) 11, 1215, 1376, 1408
BtgI CCRYGG 1 cut(s) 1377
BtgZI GCGATG 1 cut(s) 51
BtrI CACGTC 2 cut(s) 33, 404
BtsCI GGATG 4 cut(s) 115, 382, 1491, 1573
BtsIMutI CAGTG 4 cut(s) 336, 976, 1396, 1473
Cac8I GCNNGC 2 cut(s) 450, 454
CaiI CAGNNNCTG 1 cut(s) 809
CciI TCATGA 1 cut(s) 670
CfoI GCGC 2 cut(s) 165, 355
Cfr13I GGNCC 5 cut(s) 410, 642, 703, 1374, 1406
CseI GACGC 1 cut(s) 1465
Csp6I GTAC 2 cut(s) 392, 724
CspCI CAANNNNNGTGG 2 cut(s) 619, 654
CviQI GTAC 2 cut(s) 392, 724
DdeI CTNAG 4 cut(s) 210, 819, 888, 1356
DpnI GATC 9 cut(s) 82, 381, 488, 815, 1242, 1290, 1313, 1482, 1580
DpnII GATC 9 cut(s) 80, 379, 486, 813, 1240, 1288, 1311, 1480, 1578
DrdI GACNNNNNNGTC 1 cut(s) 224
DseDI GACNNNNNNGTC 1 cut(s) 224
EaeI YGGCCR 1 cut(s) 9
Eam1104I CTCTTC 2 cut(s) 1077, 1353
EarI CTCTTC 2 cut(s) 1077, 1353
Eco130I CCWWGG 1 cut(s) 1377
Eco32I GATATC 1 cut(s) 625
Eco47I GGWCC 3 cut(s) 410, 642, 703
Eco88I CYCGRG 1 cut(s) 414
EcoO109I RGGNCCY 2 cut(s) 410, 642
EcoRII CCWGG 1 cut(s) 481
EcoRV GATATC 1 cut(s) 625
EcoT14I CCWWGG 1 cut(s) 1377
EcoT22I ATGCAT 2 cut(s) 1186, 1327
ErhI CCWWGG 1 cut(s) 1377
Esp3I CGTCTC 3 cut(s) 24, 42, 1448
FalI AAGNNNNNCTT 4 cut(s) 534, 566, 1074, 1106
FaqI GGGAC 1 cut(s) 655
FbaI TGATCA 1 cut(s) 1578
FblI GTMKAC 1 cut(s) 1420
Fnu4HI GCNGC 4 cut(s) 759, 906, 1088, 1544
FokI GGATG 4 cut(s) 102, 389, 1498, 1580
Fsp4HI GCNGC 4 cut(s) 759, 906, 1088, 1544
FspBI CTAG 3 cut(s) 1205, 1494, 1521
FspI TGCGCA 1 cut(s) 354
GlaI GCGC 2 cut(s) 164, 354
GluI GCNGC 4 cut(s) 759, 906, 1088, 1544
GsaI CCCAGC 2 cut(s) 175, 813
GsuI CTGGAG 1 cut(s) 61
HaeIII GGCC 4 cut(s) 11, 1215, 1376, 1408
HapII CCGG 1 cut(s) 112
HgaI GACGC 1 cut(s) 1465
HhaI GCGC 2 cut(s) 165, 355
Hin6I GCGC 2 cut(s) 163, 353
HinP1I GCGC 2 cut(s) 163, 353
HincII GTYRAC 1 cut(s) 603
HindII GTYRAC 1 cut(s) 603
HindIII AAGCTT 1 cut(s) 303
HinfI GANTC 6 cut(s) 5, 196, 499, 1034, 1303, 1386
HpaII CCGG 1 cut(s) 112
HphI GGTGA 1 cut(s) 749
Hpy166II GTNNAC 5 cut(s) 603, 724, 1414, 1421, 1471
Hpy188I TCNGA 9 cut(s) 120, 146, 182, 439, 891, 1260, 1330, 1359, 1485
Hpy188III TCNNGA 9 cut(s) 78, 132, 416, 503, 660, 671, 1007, 1031, 1188
Hpy8I GTNNAC 5 cut(s) 603, 724, 1414, 1421, 1471
Hpy99I CGWCG 5 cut(s) 37, 221, 277, 405, 1513
HpyAV CCTTC 4 cut(s) 60, 66, 1046, 1121
HpyCH4III ACNGT 7 cut(s) 221, 273, 496, 971, 1402, 1418, 1468
HpyCH4IV ACGT 4 cut(s) 32, 237, 403, 787
HpyCH4V TGCA 9 cut(s) 407, 456, 908, 1151, 1162, 1184, 1325, 1344, 1505
HpyF10VI GCNNNNNNNGC 7 cut(s) 52, 893, 902, 989, 998, 1130, 1361
HpyF3I CTNAG 4 cut(s) 210, 819, 888, 1356
HpySE526I ACGT 4 cut(s) 32, 237, 403, 787
HspAI GCGC 2 cut(s) 163, 353
Ksp22I TGATCA 1 cut(s) 1578
Kzo9I GATC 9 cut(s) 80, 379, 486, 813, 1240, 1288, 1311, 1480, 1578
LguI GCTCTTC 1 cut(s) 1077
LmnI GCTCC 3 cut(s) 80, 755, 1130
Lsp1109I GCAGC 3 cut(s) 770, 892, 1530
LweI GCATC 6 cut(s) 124, 125, 1171, 1193, 1334, 1514
MaeI CTAG 3 cut(s) 1205, 1494, 1521
MaeII ACGT 4 cut(s) 32, 237, 403, 787
MaeIII GTNAC 3 cut(s) 96, 460, 1447
MalI GATC 9 cut(s) 82, 381, 488, 815, 1242, 1290, 1313, 1482, 1580
MbiI CCGCTC 1 cut(s) 1087
MboI GATC 9 cut(s) 80, 379, 486, 813, 1240, 1288, 1311, 1480, 1578
MboII GAAGA 8 cut(s) 245, 554, 641, 785, 893, 1094, 1273, 1340
MfeI CAATTG 1 cut(s) 825
MflI RGATCY 3 cut(s) 80, 813, 1288
MluCI AATT 9 cut(s) 265, 507, 555, 729, 765, 825, 945, 1073, 1437
MmeI TCCRAC 2 cut(s) 922, 1316
Mph1103I ATGCAT 2 cut(s) 1186, 1327
MroXI GAANNNNTTC 1 cut(s) 237
MseI TTAA 3 cut(s) 900, 1092, 1236
MslI CAYNNNNRTG 1 cut(s) 633
MspI CCGG 1 cut(s) 112
MspR9I CCNGG 1 cut(s) 483
MunI CAATTG 1 cut(s) 825
MvaI CCWGG 1 cut(s) 483
MvnI CGCG 1 cut(s) 163
MwoI GCNNNNNNNGC 7 cut(s) 52, 893, 902, 989, 998, 1130, 1361
NcoI CCATGG 1 cut(s) 1377
NdeII GATC 9 cut(s) 80, 379, 486, 813, 1240, 1288, 1311, 1480, 1578
NlaIV GGNNCC 6 cut(s) 76, 643, 745, 815, 1029, 1375
NmuCI GTSAC 2 cut(s) 96, 460
NsbI TGCGCA 1 cut(s) 354
NsiI ATGCAT 2 cut(s) 1186, 1327
NspI RCATGY 5 cut(s) 468, 532, 914, 1109, 1427
NspV TTCGAA 2 cut(s) 194, 652
OliI CACNNNNGTG 1 cut(s) 633
PagI TCATGA 1 cut(s) 670
PasI CCCWGGG 1 cut(s) 482
PciI ACATGT 4 cut(s) 464, 528, 910, 1105
PciSI GCTCTTC 1 cut(s) 1077
PcsI WCGNNNNNNNCGW 2 cut(s) 14, 658
PdmI GAANNNNTTC 1 cut(s) 237
PfeI GAWTC 6 cut(s) 5, 196, 499, 1034, 1303, 1386
PkrI GCNGC 4 cut(s) 760, 907, 1089, 1545
PpuMI RGGWCCY 2 cut(s) 410, 642
PscI ACATGT 4 cut(s) 464, 528, 910, 1105
PshBI ATTAAT 1 cut(s) 900
Psp5II RGGWCCY 2 cut(s) 410, 642
Psp6I CCWGG 1 cut(s) 481
PspFI CCCAGC 2 cut(s) 171, 809
PspGI CCWGG 1 cut(s) 481
PspN4I GGNNCC 6 cut(s) 76, 643, 745, 815, 1029, 1375
PspPI GGNCC 5 cut(s) 410, 642, 703, 1374, 1406
PspPPI RGGWCCY 2 cut(s) 410, 642
PstNI CAGNNNCTG 1 cut(s) 809
PsuI RGATCY 3 cut(s) 80, 813, 1288
RsaI GTAC 2 cut(s) 393, 725
RsaNI GTAC 2 cut(s) 392, 724
RseI CAYNNNNRTG 1 cut(s) 633
SapI GCTCTTC 1 cut(s) 1077
SaqAI TTAA 3 cut(s) 900, 1092, 1236
SatI GCNGC 4 cut(s) 759, 906, 1088, 1544
Sau3AI GATC 9 cut(s) 80, 379, 486, 813, 1240, 1288, 1311, 1480, 1578
Sau96I GGNCC 5 cut(s) 410, 642, 703, 1374, 1406
ScrFI CCNGG 1 cut(s) 483
SfaNI GCATC 6 cut(s) 124, 125, 1171, 1193, 1334, 1514
SfuI TTCGAA 2 cut(s) 194, 652
SinI GGWCC 3 cut(s) 410, 642, 703
SmiMI CAYNNNNRTG 1 cut(s) 633
Sse9I AATT 9 cut(s) 265, 507, 555, 729, 765, 825, 945, 1073, 1437
SsiI CCGC 2 cut(s) 23, 1087
SspMI CTAG 3 cut(s) 1205, 1494, 1521
StyD4I CCNGG 1 cut(s) 481
StyI CCWWGG 1 cut(s) 1377
TaaI ACNGT 7 cut(s) 221, 273, 496, 971, 1402, 1418, 1468
TaiI ACGT 4 cut(s) 35, 240, 406, 790
TaqI TCGA 7 cut(s) 194, 231, 400, 502, 652, 1006, 1508
TaqII GACCGA 1 cut(s) 720
TasI AATT 9 cut(s) 265, 507, 555, 729, 765, 825, 945, 1073, 1437
TatI WGTACW 1 cut(s) 723
TauI GCSGC 1 cut(s) 1090
TfiI GAWTC 6 cut(s) 5, 196, 499, 1034, 1303, 1386
Tru1I TTAA 3 cut(s) 900, 1092, 1236
Tru9I TTAA 3 cut(s) 900, 1092, 1236
TscAI CASTG 4 cut(s) 343, 976, 1396, 1473
TseFI GTSAC 2 cut(s) 96, 460
TseI GCWGC 3 cut(s) 758, 905, 1543
Tsp45I GTSAC 2 cut(s) 96, 460
TspDTI ATGAA 4 cut(s) 54, 456, 967, 1358
TspGWI ACGGA 2 cut(s) 141, 146
TspRI CASTG 4 cut(s) 343, 976, 1396, 1473
VpaK11BI GGWCC 3 cut(s) 410, 642, 703
VspI ATTAAT 1 cut(s) 900
XapI RAATTY 1 cut(s) 1073
XceI RCATGY 5 cut(s) 468, 532, 914, 1109, 1427
XcmI CCANNNNNNNNNTGG 1 cut(s) 635
XmiI GTMKAC 1 cut(s) 1420
XmnI GAANNNNTTC 1 cut(s) 237
XspI CTAG 3 cut(s) 1205, 1494, 1521
Zsp2I ATGCAT 2 cut(s) 1186, 1327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.