Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0008506

Physical Location & Seq

Genomic Coordinates Forward (+)

25400317 .. 25406250

Gene Structure 5934 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G63420.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2736 bp

ATGATGAAACCTGCTTCTTTGCAAGGTTTCTCCTCACATGCGTCCTCCTCTATCTACTCCGATGTTCGCAGGCCAGCTACTACTCCTTCGAAAATGGCAGCTTTTAGTGCCCTCTCGCTTTGTCCCTATACTTTCACCTTCCGCCAAAGCTCTCGAATTAAGTCTACCGTTTCCTGTTCCGTCACTTCTGCTCCTGCTTCTGGTACATCTTCATCCTCTAAGACGCCACGTAGAAGATCAGGTCGACTAGAAGGAGTTGGAAAAAGCATGGAGGATTCGGTGAAACGTAAAATGGAACAGTTTTATGAAGGAACCGATGGACCACCTCTCCGTATTCTTCCCATAGGAGGCCTTGGTGAAATTGGAATGAACTGTATGCTTGTTGGAAACTATGATCGTTACATTCTAATCGACGCTGGTATAATGTTCCCTGATTATGATGAGCCTGGGATCCAGAAAATAATGCCAGACACAGGATTTATCAGACGATGGAAACACAAAATTGAAGCTGTTGTTATAACGCATGGTCATGAAGATCACATTGGTGCGTTGCCTTGGGTTATCCCAGCATTGGACCCTAATACACCAATATTCGCATCATCCTTCACCATGGAGCTTATAAAGAAGCGCTTGAAGGAGCATGGAATCTTCGTTCAATCTAGACTTAAGACATTCAGCACTCGAAGGAGATTTATGGCTGGACCATTTGAGATAGAGCCCATTACAGTTACCCATTCTATTCCTGATTGTAGTGGTTTATTCCTCCGTTGCGCTGATGGTAATATTCTTCACACTGGTGATTGGAAGATTGATGAAGCACCATTGGATGGAAAAGTCTTTGATCGTGAAGCTTTAGAAGAACTCTCAAAGGAAGGAGTCACATTGATGATGAGTGACTCAACAAATGTGTTGTCACCTGGAAGGACAATTAGTGAAAAGGTGGTAGCAGATGCTCTGGTAAGGAATGTAATGGCGGCCAAGGGAAGAGTAATCACAACTCAGTTTGCCTCTAATATACACCGTTTGGGAAGTATAAAAGCTGCTGCTGATATAACTGGTCGAAAGTTGGTCTTTGTTGGCATGTCGTTGCGGACATACCTAGAGGCAGCTTGGAGGGATGGAAAGGCTCCAATTGACCCGTCAAGTCTGATAAAAGTCGAAGACATTGAAGCATATGCTCCTAAGGACTTACTGATTGTCACAACTGGATCACAAGCAGAACCACGCGCTGCCCTGAATCTTGCATCATATGGGAGTAGTCACGCATTCAAACTTACGAAGGAAGACATAATTCTTTACTCTGCCAAGGTAATCCCAGGCAATGAATCACGAGTAATGAAAATGATGAATCGGATAGCAGATATCGGGCCAAATATTATCATGGGTAAGAATGAAATGCTCCACACATCTGGTCATGCCTACCGTGGAGAATTGGAAGAGGTCCTTAAAATAGTGAAGCCGCAACATTTTCTTCCCATACATGGAGAACTTTTGTTTCTTAAGGAGCATGAGTTGCTCGGGAAGTCTACTGGGATTAGACACACTACTGTTATAAAGAACGGAGAAATGCTAGGAGTTTCTCACCTGAGAAATAGAAGGGTTTTGTCTAATGGATTTAGCTCTCTTGGCAGGGAGAATTTGCAGTTAATGTATAGTGATGGTGATAAAGCATTTGGCACATCAAGCGAACTATGCATTGACGAACGACTTAGAATTTCTTCTGATGGCATTATAGTTCTGAGCATGGAGATCATGCGTCCAGGGGTCTCAGAAAACACTTTGAAAGGAAAAATAAGAATCACAACGCGGTGCATGTGGCTTGACAAAGGAAGACTTTTAGATGCACTTCACAAAGCAGCTCATGCTGCTCTATCAAGTTGTCCTGTGACATGTCCCTTGTCTCACATGGAAAGAACAGTCTCCGAAGTCCTGAGGAAGATTGTAAGGAAGTACAGTGGTAAAAGGCCTGAAGTCATCGCTATAGCCACTGAGAATCCAATGGCTGTCCGAGCTGATGAGGTCAGTGCGAGACTGTCTGGTGATCCAAGTGTTGGTTCTGGAGTTGCAGCTTTAAGGAAAGTTGTTGAAGGAAATGACAAAAGAAGTCGGGCGAAGAAAGCACCTTCACAAGAAGCTTCCCCCAAAGAAGTAGATCGCACTTTGGAAGATGATATCATTGATAGTGCAAGACTACTGGCTGAAGAAGAAACTGCGGCATCAACATACACGGAAGAAGTTGATACGCCCGTTGGGAGTTCTTCAGAAGAGTCAGACGATTTTTGGAAATCATTCATCAATCCATCATCGTCACCTTCACCGAGTGAAACAGAAAATATGAATAAGGTAGCTGATACGGAGCCTAAAGCAGAGGGTAAGGAAAACAGCAGAGACGACGATGAATTAGCTGATGCTTCAGATTCTGAAACCAAGTCATCACCAAAACGTGTGAGGAAGAACAAATGGAAACCGGAGGAGATAAAGAAGGTAATCAGAATGCGAGGAGAGCTGCACAGTAGATTTCAAGTGGTGAAAGGTAGAATGGCATTGTGGGAAGAGATCTCTTCAAATCTATCAGCTGAAGGAATCAATCGAAGCCCGGGACAATGCAAATCTCTCTGGGCATCACTTATTCAGAAATACGAGGAGAGCAAGGCTGATGAGAGAAGCAAGACGAGTTGGCCACATTTTGAGGATATGAACAACATTTTGTCAGAGCTAGGCACACCTGCGTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000578 GO:0003006 GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004527 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0006810 GO:0006996 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0008409 GO:0009314 GO:0009416 GO:0009507 GO:0009536 GO:0009628 GO:0009657 GO:0009658 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009880 GO:0009914 GO:0009942 GO:0009987 GO:0010154 GO:0010817 GO:0016043 GO:0016787 GO:0016788 GO:0022414 GO:0032501 GO:0032502 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0051179 GO:0051234 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Lactamase_B Lactamase_B_2 Myb_DNA-bind_4 RMMBL

Protein Analysis

911

Amino Acids

100.55

Weight (kDa)

8.42

Isoelectric Point (pI)

48.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lactamase_B	PF00753	124 - 267	1.3e-07	Metallo-beta-lactamase superfamily
Lactamase_B_2	PF12706	133 - 269	4.1e-09	Beta-lactamase superfamily domain
RNase_J_b_CASP	PF22505	327 - 452	1.6e-28	Ribonuclease J, beta-CASP domain
RMMBL	PF07521	459 - 511	2.8e-08	Zn-dependent metallo-hydrolase RNA specificity domain
Myb_DNA-bind_4	PF13837	818 - 902	4.3e-21	Myb/SANT-like DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0008506)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63420
fragaria_vesca	FvH4_5g07980 FvH4_5g07980 FvH4_5g07980 FvH4_5g07980
malus_domestica	MD06G1143600.v1.1 MD14G1158900.v1.1
prunus_persica	Prupe.5G152800_v2.0.a1 Prupe.5G152800_v2.0.a1 Prupe.5G152800_v2.0.a1
pyrus_communis	pycom06g13330 pycom14g13250
rosa_chinensis	RchiOBHm_Chr7g0190431
rosa_laevigata	RLG00000004533
rosa_multiflora	Rmu_ssc0000036.1_g000001
rosa_roxburghii	Rroxscaffold_3G00264630
rosa_rugosa	Rorug06G0504700 Rorug06G0504700
rosa_samantha	Rh7AG110500 Rh7BG113100 Rh7CG115300
rosa_wichuraiana	Rw0G019850 Rw7G009590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	518, 620, 1553
Acc36I	ACCTGC	1 cut(s)	19
AccB7I	CCANNNNNTGG	2 cut(s)	827, 2051
AccI	GTMKAC	3 cut(s)	164, 244, 1526
AccII	CGCG	2 cut(s)	1227, 1807
AciI	CCGC	6 cut(s)	142, 974, 1090, 1460, 1807, 2213
AclWI	GGATC	4 cut(s)	445, 458, 1216, 2036
AcoI	YGGCCR	2 cut(s)	975, 2678
AcsI	RAATTY	2 cut(s)	1636, 1713
AcuI	CTGAAG	5 cut(s)	1989, 2220, 2244, 2397, 2598
AcyI	GRCGYC	1 cut(s)	224
AdeI	CACNNNGTG	1 cut(s)	2321
AfaI	GTAC	2 cut(s)	205, 1952
AfeI	AGCGCT	1 cut(s)	629
AfiI	CCNNNNNNNGG	7 cut(s)	200, 347, 473, 571, 827, 1481, 2051
AflII	CTTAAG	2 cut(s)	665, 1499
AflIII	ACRYGT	2 cut(s)	1889, 2443
AgsI	TTSAA	9 cut(s)	506, 634, 656, 1169, 1270, 1783, 2087, 2522, 2565
AjnI	CCWGG	4 cut(s)	445, 916, 1315, 1759
AjuI	GAANNNNNNNTTGG	4 cut(s)	525, 557, 2038, 2070
AleI	CACNNNNGTG	2 cut(s)	543, 795
AloI	GAACNNNNNNTCC	2 cut(s)	636, 668
Alw26I	GTCTC	5 cut(s)	1771, 1905, 1924, 2023, 2382
AlwI	GGATC	4 cut(s)	445, 458, 1216, 2036
AlwNI	CAGNNNCTG	1 cut(s)	2420
Ama87I	CYCGRG	2 cut(s)	1517, 2596
Aor51HI	AGCGCT	1 cut(s)	629
AoxI	GGCC	6 cut(s)	71, 349, 975, 1367, 1964, 2678
ApeKI	GCWGC	9 cut(s)	98, 1040, 1043, 1106, 1229, 1856, 1865, 2066, 2506
ApoI	RAATTY	2 cut(s)	1636, 1713
ArsI	GACNNNNNNTTYG	2 cut(s)	896, 928
Asp700I	GAANNNNTTC	2 cut(s)	1717, 2288
AspLEI	GCGC	3 cut(s)	630, 773, 1229
AspS9I	GGNCC	5 cut(s)	320, 574, 701, 1367, 1441
AsuC2I	CCSGG	2 cut(s)	2597, 2598
AsuII	TTCGAA	1 cut(s)	89
AvaI	CYCGRG	2 cut(s)	1517, 2596
AvaII	GGWCC	4 cut(s)	320, 574, 701, 1441
AxyI	CCTNAGG	2 cut(s)	1182, 1931
BaeGI	GKGCMC	1 cut(s)	112
BaeI	ACNNNNGTAYC	2 cut(s)	2232, 2265
BalI	TGGCCA	1 cut(s)	2680
BamHI	GGATCC	1 cut(s)	450
BanII	GRGCYC	1 cut(s)	720
BauI	CACGAG	1 cut(s)	1329
BbsI	GAAGAC	3 cut(s)	1167, 1290, 1837
BbvI	GCAGC	9 cut(s)	110, 1027, 1030, 1118, 1216, 1852, 1868, 2078, 2493
BccI	CCATC	8 cut(s)	311, 483, 770, 821, 1112, 1652, 1718, 2308
BciT130I	CCWGG	4 cut(s)	447, 918, 1317, 1761
BcnI	CCSGG	2 cut(s)	2597, 2598
BcoDI	GTCTC	5 cut(s)	1771, 1905, 1924, 2023, 2382
BfaI	CTAG	5 cut(s)	248, 660, 1100, 1571, 2717
BfmI	CTRYAG	1 cut(s)	1980
BfoI	RGCGCY	1 cut(s)	631
BfrI	CTTAAG	2 cut(s)	665, 1499
BfuAI	ACCTGC	1 cut(s)	19
BglII	AGATCT	1 cut(s)	2556
Bme1390I	CCNGG	6 cut(s)	447, 918, 1317, 1761, 2597, 2598
Bme18I	GGWCC	4 cut(s)	320, 574, 701, 1441
BmeT110I	CYCGRG	2 cut(s)	1517, 2596
BmgT120I	GGNCC	5 cut(s)	320, 574, 701, 1367, 1441
BmiI	GGNNCC	5 cut(s)	313, 452, 576, 1128, 2358
BmrFI	CCNGG	6 cut(s)	447, 918, 1317, 1761, 2597, 2598
BmrI	ACTGGG	1 cut(s)	1539
BmsI	GCATC	7 cut(s)	605, 940, 1253, 1831, 2225, 2398, 2630
BmuI	ACTGGG	1 cut(s)	1539
BpiI	GAAGAC	3 cut(s)	1167, 1290, 1837
BpmI	CTGGAG	1 cut(s)	2079
Bpu14I	TTCGAA	1 cut(s)	89
BpuMI	CCSGG	2 cut(s)	2597, 2598
BsaAI	YACGTR	1 cut(s)	230
BsaHI	GRCGYC	1 cut(s)	224
BsaI	GGTCTC	1 cut(s)	1771
BsaWI	WCCGGW	1 cut(s)	2467
BsaXI	ACNNNNNCTCC	6 cut(s)	175, 205, 312, 342, 1496, 1526
Bsc4I	CCNNNNNNNGG	7 cut(s)	200, 347, 473, 571, 827, 1481, 2051
Bse1I	ACTGG	5 cut(s)	799, 1060, 1210, 1534, 2199
Bse21I	CCTNAGG	2 cut(s)	1182, 1931
Bse3DI	GCAATG	1 cut(s)	1327
BseBI	CCWGG	4 cut(s)	447, 918, 1317, 1761
BseGI	GGATG	4 cut(s)	212, 599, 832, 1123
BseLI	CCNNNNNNNGG	7 cut(s)	200, 347, 473, 571, 827, 1481, 2051
BseMI	GCAATG	1 cut(s)	1327
BseMII	CTCAG	6 cut(s)	1013, 1577, 1730, 1782, 1922, 1980
BseNI	ACTGG	5 cut(s)	799, 1060, 1210, 1534, 2199
BseRI	GAGGAG	5 cut(s)	22, 37, 2486, 2514, 2657
BseSI	GKGCMC	1 cut(s)	112
BseXI	GCAGC	9 cut(s)	110, 1027, 1030, 1118, 1216, 1852, 1868, 2078, 2493
BseYI	CCCAGC	1 cut(s)	565
BsgI	GTGCAG	1 cut(s)	2492
Bsh1236I	CGCG	2 cut(s)	1227, 1807
BshFI	GGCC	6 cut(s)	73, 351, 977, 1369, 1966, 2680
BsiHKCI	CYCGRG	2 cut(s)	1517, 2596
BsiSI	CCGG	2 cut(s)	2468, 2597
BslFI	GGGAC	3 cut(s)	108, 1878, 2613
BslI	CCNNNNNNNGG	7 cut(s)	200, 347, 473, 571, 827, 1481, 2051
BsmAI	GTCTC	5 cut(s)	1771, 1905, 1924, 2023, 2382
BsmBI	CGTCTC	1 cut(s)	2382
BsmFI	GGGAC	3 cut(s)	108, 1878, 2613
BsmI	GAATGC	2 cut(s)	1265, 2499
BsnI	GGCC	6 cut(s)	73, 351, 977, 1369, 1966, 2680
Bso31I	GGTCTC	1 cut(s)	1771
BsoBI	CYCGRG	2 cut(s)	1517, 2596
Bsp119I	TTCGAA	1 cut(s)	89
Bsp1286I	GDGCHC	2 cut(s)	112, 720
Bsp19I	CCATGG	1 cut(s)	609
BspACI	CCGC	6 cut(s)	142, 974, 1090, 1460, 1807, 2213
BspANI	GGCC	6 cut(s)	73, 351, 977, 1369, 1966, 2680
BspCNI	CTCAG	6 cut(s)	1012, 1578, 1731, 1781, 1923, 1981
BspFNI	CGCG	2 cut(s)	1227, 1807
BspHI	TCATGA	1 cut(s)	529
BspLI	GGNNCC	5 cut(s)	313, 452, 576, 1128, 2358
BspMI	ACCTGC	1 cut(s)	19
BspPI	GGATC	4 cut(s)	445, 458, 1216, 2036
BspT104I	TTCGAA	1 cut(s)	89
BspTI	CTTAAG	2 cut(s)	665, 1499
BspTNI	GGTCTC	1 cut(s)	1771
BsrDI	GCAATG	1 cut(s)	1327
BsrI	ACTGG	5 cut(s)	799, 1060, 1210, 1534, 2199
BssNI	GRCGYC	1 cut(s)	224
BssSI	CACGAG	1 cut(s)	1329
BssT1I	CCWWGG	5 cut(s)	352, 554, 609, 978, 1305
Bst2BI	CACGAG	1 cut(s)	1329
Bst2UI	CCWGG	4 cut(s)	447, 918, 1317, 1761
Bst6I	CTCTTC	5 cut(s)	979, 1431, 2259, 2547, 2566
BstACI	GRCGYC	1 cut(s)	224
BstAFI	CTTAAG	2 cut(s)	665, 1499
BstAPI	GCANNNNNTGC	2 cut(s)	1513, 1862
BstBAI	YACGTR	1 cut(s)	230
BstBI	TTCGAA	1 cut(s)	89
BstC8I	GCNNGC	2 cut(s)	71, 75
BstDEI	CTNAG	9 cut(s)	219, 999, 1182, 1586, 1709, 1739, 1768, 1931, 1989
BstDSI	CCRYGG	2 cut(s)	609, 1423
BstF5I	GGATG	4 cut(s)	212, 599, 832, 1123
BstFNI	CGCG	2 cut(s)	1227, 1807
BstH2I	RGCGCY	1 cut(s)	631
BstHHI	GCGC	3 cut(s)	630, 773, 1229
BstMAI	GTCTC	5 cut(s)	1771, 1905, 1924, 2023, 2382
BstNI	CCWGG	4 cut(s)	447, 918, 1317, 1761
BstNSI	RCATGY	4 cut(s)	41, 1084, 1816, 1893
BstSCI	CCNGG	6 cut(s)	445, 916, 1315, 1759, 2595, 2596
BstSFI	CTRYAG	1 cut(s)	1980
BstSLI	GKGCMC	1 cut(s)	112
BstUI	CGCG	2 cut(s)	1227, 1807
BstV1I	GCAGC	9 cut(s)	110, 1027, 1030, 1118, 1216, 1852, 1868, 2078, 2493
BstV2I	GAAGAC	3 cut(s)	1167, 1290, 1837
BstX2I	RGATCY	2 cut(s)	450, 2556
BstXI	CCANNNNNNTGG	1 cut(s)	1409
BstYI	RGATCY	2 cut(s)	450, 2556
Bsu36I	CCTNAGG	2 cut(s)	1182, 1931
BsuRI	GGCC	6 cut(s)	73, 351, 977, 1369, 1966, 2680
BtgI	CCRYGG	2 cut(s)	609, 1423
BtgZI	GCGATG	1 cut(s)	1960
BtsCI	GGATG	4 cut(s)	212, 599, 832, 1123
BtsIMutI	CAGTG	4 cut(s)	792, 1960, 1986, 2029
BveI	ACCTGC	1 cut(s)	19
Cac8I	GCNNGC	2 cut(s)	71, 75
CaiI	CAGNNNCTG	1 cut(s)	2420
CciI	TCATGA	1 cut(s)	529
CfoI	GCGC	3 cut(s)	630, 773, 1229
Cfr13I	GGNCC	5 cut(s)	320, 574, 701, 1367, 1441
Cfr9I	CCCGGG	1 cut(s)	2596
CseI	GACGC	5 cut(s)	30, 232, 422, 1745, 2718
Csp6I	GTAC	2 cut(s)	204, 1951
CviQI	GTAC	2 cut(s)	204, 1951
DdeI	CTNAG	9 cut(s)	219, 999, 1182, 1586, 1709, 1739, 1768, 1931, 1989
DraIII	CACNNNGTG	1 cut(s)	2321
EaeI	YGGCCR	2 cut(s)	975, 2678
Eam1104I	CTCTTC	5 cut(s)	979, 1431, 2259, 2547, 2566
EarI	CTCTTC	5 cut(s)	979, 1431, 2259, 2547, 2566
EciI	GGCGGA	1 cut(s)	131
Eco130I	CCWWGG	5 cut(s)	352, 554, 609, 978, 1305
Eco147I	AGGCCT	2 cut(s)	351, 1966
Eco24I	GRGCYC	1 cut(s)	720
Eco31I	GGTCTC	1 cut(s)	1771
Eco32I	GATATC	2 cut(s)	1363, 2173
Eco47I	GGWCC	4 cut(s)	320, 574, 701, 1441
Eco47III	AGCGCT	1 cut(s)	629
Eco57I	CTGAAG	5 cut(s)	1989, 2220, 2244, 2397, 2598
Eco81I	CCTNAGG	2 cut(s)	1182, 1931
Eco88I	CYCGRG	2 cut(s)	1517, 2596
EcoO109I	RGGNCCY	1 cut(s)	1441
EcoRII	CCWGG	4 cut(s)	445, 916, 1315, 1759
EcoRV	GATATC	2 cut(s)	1363, 2173
EcoT14I	CCWWGG	5 cut(s)	352, 554, 609, 978, 1305
EcoT22I	ATGCAT	1 cut(s)	1697
EcoT38I	GRGCYC	1 cut(s)	720
ErhI	CCWWGG	5 cut(s)	352, 554, 609, 978, 1305
Esp3I	CGTCTC	1 cut(s)	2382
FalI	AAGNNNNNCTT	8 cut(s)	614, 646, 1055, 1087, 1428, 1460, 1818, 1850
FaqI	GGGAC	3 cut(s)	108, 1878, 2613
FauNDI	CATATG	2 cut(s)	1174, 1249
FblI	GTMKAC	3 cut(s)	164, 244, 1526
FokI	GGATG	4 cut(s)	199, 586, 839, 1130
FriOI	GRGCYC	1 cut(s)	720
FspBI	CTAG	5 cut(s)	248, 660, 1100, 1571, 2717
GlaI	GCGC	3 cut(s)	629, 772, 1228
GsaI	CCCAGC	1 cut(s)	569
GsuI	CTGGAG	1 cut(s)	2079
HaeII	RGCGCY	1 cut(s)	631
HaeIII	GGCC	6 cut(s)	73, 351, 977, 1369, 1966, 2680
HapII	CCGG	2 cut(s)	2468, 2597
HgaI	GACGC	5 cut(s)	30, 232, 422, 1745, 2718
HhaI	GCGC	3 cut(s)	630, 773, 1229
Hin1I	GRCGYC	1 cut(s)	224
Hin6I	GCGC	3 cut(s)	628, 771, 1227
HinP1I	GCGC	3 cut(s)	628, 771, 1227
HincII	GTYRAC	1 cut(s)	245
HindII	GTYRAC	1 cut(s)	245
HindIII	AAGCTT	2 cut(s)	849, 2133
HpaII	CCGG	2 cut(s)	2468, 2597
Hpy166II	GTNNAC	3 cut(s)	165, 245, 1527
Hpy8I	GTNNAC	3 cut(s)	165, 245, 1527
Hpy99I	CGWCG	2 cut(s)	416, 2396
HpyCH4IV	ACGT	3 cut(s)	229, 286, 2443
HpyF3I	CTNAG	9 cut(s)	219, 999, 1182, 1586, 1709, 1739, 1768, 1931, 1989
HpySE526I	ACGT	3 cut(s)	229, 286, 2443
Hsp92I	GRCGYC	1 cut(s)	224
HspAI	GCGC	3 cut(s)	628, 771, 1227
LmnI	GCTCC	8 cut(s)	196, 613, 637, 1132, 1183, 1404, 1504, 2356
Lsp1109I	GCAGC	9 cut(s)	110, 1027, 1030, 1118, 1216, 1852, 1868, 2078, 2493
LweI	GCATC	7 cut(s)	605, 940, 1253, 1831, 2225, 2398, 2630
MaeI	CTAG	5 cut(s)	248, 660, 1100, 1571, 2717
MaeII	ACGT	3 cut(s)	229, 286, 2443
MfeI	CAATTG	1 cut(s)	1131
MflI	RGATCY	2 cut(s)	450, 2556
MhlI	GDGCHC	2 cut(s)	112, 720
MlsI	TGGCCA	1 cut(s)	2680
MluNI	TGGCCA	1 cut(s)	2680
MlyI	GAGTC	3 cut(s)	885, 890, 2276
MmeI	TCCRAC	2 cut(s)	238, 364
Mox20I	TGGCCA	1 cut(s)	2680
Mph1103I	ATGCAT	1 cut(s)	1697
MroXI	GAANNNNTTC	2 cut(s)	1717, 2288
MscI	TGGCCA	1 cut(s)	2680
MseI	TTAA	7 cut(s)	159, 666, 1446, 1500, 1646, 2072, 2734
MslI	CAYNNNNRTG	4 cut(s)	528, 543, 795, 884
Msp20I	TGGCCA	1 cut(s)	2680
MspA1I	CMGCKG	1 cut(s)	2576
MspCI	CTTAAG	2 cut(s)	665, 1499
MspI	CCGG	2 cut(s)	2468, 2597
MspR9I	CCNGG	6 cut(s)	447, 918, 1317, 1761, 2597, 2598
MunI	CAATTG	1 cut(s)	1131
Mva1269I	GAATGC	2 cut(s)	1265, 2499
MvaI	CCWGG	4 cut(s)	447, 918, 1317, 1761
MvnI	CGCG	2 cut(s)	1227, 1807
NciI	CCSGG	2 cut(s)	2597, 2598
NcoI	CCATGG	1 cut(s)	609
NdeI	CATATG	2 cut(s)	1174, 1249
NlaIV	GGNNCC	5 cut(s)	313, 452, 576, 1128, 2358
NmuCI	GTSAC	8 cut(s)	181, 877, 893, 912, 1198, 1259, 1885, 2307
NsiI	ATGCAT	1 cut(s)	1697
NspI	RCATGY	4 cut(s)	41, 1084, 1816, 1893
NspV	TTCGAA	1 cut(s)	89
OliI	CACNNNNGTG	2 cut(s)	543, 795
PagI	TCATGA	1 cut(s)	529
PceI	AGGCCT	2 cut(s)	351, 1966
PciI	ACATGT	1 cut(s)	1889
PctI	GAATGC	2 cut(s)	1265, 2499
PdmI	GAANNNNTTC	2 cut(s)	1717, 2288
PfeI	GAWTC	9 cut(s)	275, 645, 1237, 1325, 1348, 1797, 1993, 2417, 2583
PflMI	CCANNNNNTGG	2 cut(s)	827, 2051
PleI	GAGTC	3 cut(s)	884, 890, 2275
PpsI	GAGTC	3 cut(s)	884, 890, 2275
Ppu21I	YACGTR	1 cut(s)	230
PpuMI	RGGWCCY	1 cut(s)	1441
PscI	ACATGT	1 cut(s)	1889
PsiI	TTATAA	3 cut(s)	518, 620, 1553
Psp5II	RGGWCCY	1 cut(s)	1441
Psp6I	CCWGG	4 cut(s)	445, 916, 1315, 1759
PspFI	CCCAGC	1 cut(s)	565
PspGI	CCWGG	4 cut(s)	445, 916, 1315, 1759
PspN4I	GGNNCC	5 cut(s)	313, 452, 576, 1128, 2358
PspPI	GGNCC	5 cut(s)	320, 574, 701, 1367, 1441
PspPPI	RGGWCCY	1 cut(s)	1441
PstNI	CAGNNNCTG	1 cut(s)	2420
PsuI	RGATCY	2 cut(s)	450, 2556
PvuII	CAGCTG	1 cut(s)	2576
RsaI	GTAC	2 cut(s)	205, 1952
RsaNI	GTAC	2 cut(s)	204, 1951
RseI	CAYNNNNRTG	4 cut(s)	528, 543, 795, 884
SalI	GTCGAC	1 cut(s)	243
SaqAI	TTAA	7 cut(s)	159, 666, 1446, 1500, 1646, 2072, 2734
Sau96I	GGNCC	5 cut(s)	320, 574, 701, 1367, 1441
SchI	GAGTC	3 cut(s)	885, 890, 2276
ScrFI	CCNGG	6 cut(s)	447, 918, 1317, 1761, 2597, 2598
SduI	GDGCHC	2 cut(s)	112, 720
SfaNI	GCATC	7 cut(s)	605, 940, 1253, 1831, 2225, 2398, 2630
SfcI	CTRYAG	1 cut(s)	1980
SfuI	TTCGAA	1 cut(s)	89
SinI	GGWCC	4 cut(s)	320, 574, 701, 1441
SmaI	CCCGGG	1 cut(s)	2598
SmiMI	CAYNNNNRTG	4 cut(s)	528, 543, 795, 884
SmlI	CTYRAG	2 cut(s)	665, 1499
SmoI	CTYRAG	2 cut(s)	665, 1499
SseBI	AGGCCT	2 cut(s)	351, 1966
SsiI	CCGC	6 cut(s)	142, 974, 1090, 1460, 1807, 2213
SspI	AATATT	3 cut(s)	591, 784, 1375
SspMI	CTAG	5 cut(s)	248, 660, 1100, 1571, 2717
StuI	AGGCCT	2 cut(s)	351, 1966
StyD4I	CCNGG	6 cut(s)	445, 916, 1315, 1759, 2595, 2596
StyI	CCWWGG	5 cut(s)	352, 554, 609, 978, 1305
TaiI	ACGT	3 cut(s)	232, 289, 2446
TaqI	TCGA	8 cut(s)	89, 154, 244, 411, 682, 1060, 1158, 2590
TatI	WGTACW	1 cut(s)	1950
TauI	GCSGC	3 cut(s)	977, 1462, 2216
TfiI	GAWTC	9 cut(s)	275, 645, 1237, 1325, 1348, 1797, 1993, 2417, 2583
Tru1I	TTAA	7 cut(s)	159, 666, 1446, 1500, 1646, 2072, 2734
Tru9I	TTAA	7 cut(s)	159, 666, 1446, 1500, 1646, 2072, 2734
TscAI	CASTG	4 cut(s)	799, 1960, 1993, 2029
TseFI	GTSAC	8 cut(s)	181, 877, 893, 912, 1198, 1259, 1885, 2307
TseI	GCWGC	9 cut(s)	98, 1040, 1043, 1106, 1229, 1856, 1865, 2066, 2506
Tsp45I	GTSAC	8 cut(s)	181, 877, 893, 912, 1198, 1259, 1885, 2307
TspGWI	ACGGA	6 cut(s)	169, 320, 755, 1575, 2243, 2369
TspMI	CCCGGG	1 cut(s)	2596
TspRI	CASTG	4 cut(s)	799, 1960, 1993, 2029
Van91I	CCANNNNNTGG	2 cut(s)	827, 2051
Vha464I	CTTAAG	2 cut(s)	665, 1499
VpaK11BI	GGWCC	4 cut(s)	320, 574, 701, 1441
XapI	RAATTY	2 cut(s)	1636, 1713
XbaI	TCTAGA	1 cut(s)	659
XceI	RCATGY	4 cut(s)	41, 1084, 1816, 1893
XmaI	CCCGGG	1 cut(s)	2596
XmiI	GTMKAC	3 cut(s)	164, 244, 1526
XmnI	GAANNNNTTC	2 cut(s)	1717, 2288
XspI	CTAG	5 cut(s)	248, 660, 1100, 1571, 2717
Zsp2I	ATGCAT	1 cut(s)	1697

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.