Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0008506

Physical Location & Seq

Genomic Coordinates Reverse (-)

28712475 .. 28720183

Gene Structure 7709 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1143600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2754 bp

ATGCAATTAGGGGTTTTGGGGGGTTTATCTGTCTACCATTCTCTCTACCCAGCTCCGTTCAAACCAAGCGTGTCGGCCCAAATGGCTGCTCTCGGAGTTCTCTCTCCGTGCCCTTACAGCCTCCTGTGGCGGCCGAAAGCCGCGGGCCGCTGCGTTTCGTGCTCCGTTGGCTCTTCTACTGCAGCAGGAACACATGGGTCGAATGTAAGAAGATCAGGAAGAATGGAAGGGCCTCGAAAAAGTATGGAAGACTCTGTTAAACGAAAGATGGAACAATTTTATGAAGGCAAAGAGGGCCCGCCAATCCGTGTTTTACCAATTGGTGGCCTGGGAGAAATTGGGATGAATTGCATGCTGGTTGGGAATTATGACCGCTATATTCTGATTGATGCTGGTGTTATGTTTCCAGACTTTGATGAGCTCGGAGTCCAAAAGATTATACCTGATACCACATTTATCAAAAAATGGAGCCACAAAATTGAAGCGATTGTTATTACACATGGGCATGAAGACCACATTGGTGCGTTGCCTTGGGTGATCCCAGCTTTGGATCTTGATACACCAATATTTGCATCATCCTTTACCATGGAGCTTATTAAAAAACGCCTGAAGGAACATGGGATTTTTGTTCCGTCAAGACTTAGGACATTTAGAACAAAGAGGAAATTTACGGCTGGACCATTTGAGATTGAGCCTATTCGGGTGACACATTCTATTCCTGACTGTTGTGGATTAGTTCTTCGCTGTTCTGATGGGACAATTCTTCATACTGGGGACTGGAAGATTGACGAGTCACCATTGGATGGTAGAGGTTTTGACCGTGAAGCTTTGGAAGAACTTTCAAAAGAAGGAGTAACATTGATGATGAGCGACTCTACAAATGTACTCTCACCGGGAAGGACAACTAGCGAATCTTCTGTAGCAGATGCGTTGTTGAGGCATATTTCAGCTGCTAAAGGGAGGGTTATAACTACTCAGTTTGCTTCAAACATACACCGACTTGGAAGTGTGAAAGCTGCTGCTGATTTCACCGGCAGAAAGTTGGTATTCGTTGGTATGTCCTTGAGGACATATCTTGATGCAGCTTGGAAGGATGGAAAGGCACCAATTGATCCATCATCCCTGGTGAAAGTGGAAGATATTGATTCCTATGCTCCCAAGGATTTGCTGATTGTCACTACTGGCTCTCAAGCAGAACCACGTGCTGCTCTAAATCTTGCATCATTTGGAAGTAGTCATTCCGTCAAGCTGACCAAAGAGGACGTAATTTTGTATTCAGCTAAGGTAATTCCTGGTAATGAATCTCGGGTAATGAAAATGTTAAACCGCATATCAGATCTTGGCTCAACTATAGTGATGGGTAAGAATGAGGGACTGCACACTTCTGGTCATGGGTATCGTGGAGAATTGGAAGAAGTGCTTCAAATCGTGAAGCCGCAACATTTTCTGCCGATACATGGAGAACTCTTGTTCCTCAAAGAGCATGAATTGCTTGGCAAATCAACTGGCATTCATCACACCACTGTTATAAAAAACGGGGAAATGCTTGGCGTTTCACATTTAAGAAACAGAAGAGTACTTTCTAGTGGTTTCACCATGCTTGGAAAAGAGAATGTGCAGTTGAAGTTTAGTGATGGTGATAAAGCATTTGGTACTTCAAGCGAACTGTGTGTCGATGAGAGACTAAGAATCGCCTTAGACGGCATAATAGTGGTCAGCATGGAAATTTTACGCCCTGAAAACGTAAATGGTTTGGCTGAAAATAGCATAAAGGGGAAGATAAAAATTACTACTCGATGTTTATGGCTTGACAAAGGAAAGCTTATTGATGCACTCTATAAAGCTGCCCACGCTGCACTGTCAAGCTGCCCCATAAATTGTCCTCTGGCTCACATGGAAAGAACAGTGTCTGAGGTATTGAGGAAATTGGTAAGAAAGTACAGTGGTAAAAGGCCAGACGTCATTGCCATTGCCATGGAGAACCCCGCAGTAGTCCTTGCTGATGAGGTCAGTGCAAGACTATCTGCAAAATCCCATGTTGGTTCTGAGATGTCACAGTTGAGGAAAGCATATGATCGACATCAAAATAAGGGTCCGTCAACTAGGACACAAGCAGATGACGGCAAGACACAAACATATTTTCAGAGTCAGTCAACTAGGACACAAGCAGATGGAGACAAGGACAACACATTACAGAGCATCTTGCAACAAGATACGGAAGAGTCCGAACTAGAAGATGATGGCATTCAAGTCGAAGGACTCCTACCTGAGGAAGATTCTGCTACTTCAAGTTCCAAGTCAGAGGAGCTTTCTTCTGATTCGGAGGATTTTTGGAAATCATTGGTTACTTTATCAACTGGTGACAAATCAGCTCAAGATGAAAATGGTTTGGTTGCGGAACAGGAACATCCACAGAAAGATGGTCCTGAAAACAGTGAAATACCAAATCCTTCAAAGCCTGTGAAACGCAATAGATGGAAGCCTGAGGAAGTTAAGAAGCTTATTACAATGCGGGGGAAACTGCATGGCAGATTTCAAGTTGCGAAGGGGCGAATGGCCCTCTGGGAAGAGATATCTAGAAACTTGTTGGCTGATGGGATCAACCGAAGCCCAGGACAATGCAAATCTCTGTGGGCATCCCTGGTTCAGAAATATGAGGAAAGCAAGAGCGGAAAAAGAAGTAGAAAGAGTTGGCCATACTTCGACGAAATGGATGGAGCTTTGTCTGATTCCGAGGAATTGGCAACAAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000578 GO:0003006 GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004527 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0006810 GO:0006996 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0008409 GO:0009314 GO:0009416 GO:0009507 GO:0009536 GO:0009628 GO:0009657 GO:0009658 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009880 GO:0009914 GO:0009942 GO:0009987 GO:0010154 GO:0010817 GO:0016043 GO:0016787 GO:0016788 GO:0022414 GO:0032501 GO:0032502 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0051179 GO:0051234 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Lactamase_B Lactamase_B_2 Myb_DNA-bind_4 RMMBL

Protein Analysis

918

Amino Acids

100.97

Weight (kDa)

7.62

Isoelectric Point (pI)

41.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lactamase_B	PF00753	116 - 259	4.5e-07	Metallo-beta-lactamase superfamily
Lactamase_B_2	PF12706	125 - 261	8.6e-10	Beta-lactamase superfamily domain
RNase_J_b_CASP	PF22505	319 - 444	4.1e-29	Ribonuclease J, beta-CASP domain
RMMBL	PF07521	459 - 505	1.6e-06	Zn-dependent metallo-hydrolase RNA specificity domain
Myb_DNA-bind_4	PF13837	823 - 905	2.4e-22	Myb/SANT-like DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0008506)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63420
fragaria_vesca	FvH4_5g07980 FvH4_5g07980 FvH4_5g07980 FvH4_5g07980
malus_domestica	MD06G1143600.v1.1 MD14G1158900.v1.1
prunus_persica	Prupe.5G152800_v2.0.a1 Prupe.5G152800_v2.0.a1 Prupe.5G152800_v2.0.a1
pyrus_communis	pycom06g13330 pycom14g13250
rosa_chinensis	RchiOBHm_Chr7g0190431
rosa_laevigata	RLG00000004533
rosa_multiflora	Rmu_ssc0000036.1_g000001
rosa_roxburghii	Rroxscaffold_3G00264630
rosa_rugosa	Rorug06G0504700 Rorug06G0504700
rosa_samantha	Rh7AG110500 Rh7BG113100 Rh7CG115300
rosa_wichuraiana	Rw0G019850 Rw7G009590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	968, 1529
AatII	GACGTC	1 cut(s)	1962
AccB1I	GGYRCC	1 cut(s)	1102
AccB7I	CCANNNNNTGG	2 cut(s)	323, 803
AccBSI	CCGCTC	1 cut(s)	2670
AccI	GTMKAC	1 cut(s)	33
AccII	CGCG	1 cut(s)	143
AclWI	GGATC	4 cut(s)	532, 558, 1106, 2606
AcoI	YGGCCR	2 cut(s)	131, 2693
AcsI	RAATTY	2 cut(s)	665, 1725
AcuI	CTGAAG	1 cut(s)	629
AcvI	CACGTG	1 cut(s)	1202
AcyI	GRCGYC	1 cut(s)	1959
AfaI	GTAC	4 cut(s)	885, 1578, 1654, 1940
AfiI	CCNNNNNNNGG	5 cut(s)	323, 547, 803, 1457, 2269
AjnI	CCWGG	5 cut(s)	327, 1122, 1291, 2611, 2640
AjuI	GAANNNNNNNTTGG	2 cut(s)	501, 533
AleI	CACNNNNGTG	1 cut(s)	519
AloI	GAACNNNNNNTCC	2 cut(s)	1455, 1487
Alw21I	GWGCWC	2 cut(s)	164, 423
Alw26I	GTCTC	2 cut(s)	1675, 2169
AlwI	GGATC	4 cut(s)	532, 558, 1106, 2606
AlwNI	CAGNNNCTG	1 cut(s)	1910
Ama87I	CYCGRG	1 cut(s)	1305
AoxI	GGCC	9 cut(s)	75, 131, 145, 230, 295, 325, 1952, 2556, 2693
ApaI	GGGCCC	1 cut(s)	299
ApoI	RAATTY	2 cut(s)	665, 1725
ArsI	GACNNNNNNTTYG	4 cut(s)	1656, 1688, 2077, 2109
Asp700I	GAANNNNTTC	1 cut(s)	1419
AspS9I	GGNCC	9 cut(s)	76, 145, 230, 295, 296, 677, 2093, 2423, 2557
AsuC2I	CCSGG	1 cut(s)	894
AsuHPI	GGTGA	9 cut(s)	547, 715, 786, 882, 1021, 1138, 1585, 1649, 2372
AvaI	CYCGRG	1 cut(s)	1305
AvaII	GGWCC	3 cut(s)	677, 2093, 2423
AxyI	CCTNAGG	2 cut(s)	2268, 2484
BaeGI	GKGCMC	2 cut(s)	113, 299
BalI	TGGCCA	1 cut(s)	2695
BanI	GGYRCC	1 cut(s)	1102
BanII	GRGCYC	2 cut(s)	299, 423
BarI	GAAGNNNNNNTAC	2 cut(s)	2247, 2279
BbrPI	CACGTG	1 cut(s)	1202
BbsI	GAAGAC	2 cut(s)	255, 516
Bbv12I	GWGCWC	2 cut(s)	164, 423
BceAI	ACGGC	3 cut(s)	687, 1717, 2137
BcgI	CGANNNNNNTGC	2 cut(s)	2233, 2267
BciT130I	CCWGG	5 cut(s)	329, 1124, 1293, 2613, 2642
BcnI	CCSGG	1 cut(s)	894
BcoDI	GTCTC	2 cut(s)	1675, 2169
BfaI	CTAG	6 cut(s)	906, 1584, 2103, 2157, 2231, 2577
BfmI	CTRYAG	3 cut(s)	180, 918, 1350
BglI	GCCNNNNNGGC	1 cut(s)	83
BglII	AGATCT	1 cut(s)	1336
BmcAI	AGTACT	1 cut(s)	1578
Bme1390I	CCNGG	6 cut(s)	329, 894, 1124, 1293, 2613, 2642
Bme18I	GGWCC	3 cut(s)	677, 2093, 2423
BmeT110I	CYCGRG	1 cut(s)	1305
BmgT120I	GGNCC	9 cut(s)	76, 145, 230, 295, 296, 677, 2093, 2423, 2557
BmiI	GGNNCC	4 cut(s)	297, 470, 1104, 2094
BmrFI	CCNGG	6 cut(s)	329, 894, 1124, 1293, 2613, 2642
BmrI	ACTGGG	1 cut(s)	780
BmsI	GCATC	8 cut(s)	379, 581, 916, 1069, 1229, 1819, 2208, 2645
BmuI	ACTGGG	1 cut(s)	780
BpiI	GAAGAC	2 cut(s)	255, 516
Bpu10I	CCTNAGC	1 cut(s)	1281
BpuEI	CTTGAG	3 cut(s)	1084, 1173, 2358
BpuMI	CCSGG	1 cut(s)	894
BsaAI	YACGTR	1 cut(s)	1202
BsaBI	GATNNNNATC	1 cut(s)	2079
BsaHI	GRCGYC	1 cut(s)	1959
Bsc4I	CCNNNNNNNGG	5 cut(s)	323, 547, 803, 1457, 2269
Bse118I	RCCGGY	1 cut(s)	1031
Bse1I	ACTGG	5 cut(s)	775, 782, 1186, 1510, 2362
Bse21I	CCTNAGG	2 cut(s)	2268, 2484
Bse3DI	GCAATG	2 cut(s)	1962, 1968
Bse8I	GATNNNNATC	1 cut(s)	2079
BseBI	CCWGG	5 cut(s)	329, 1124, 1293, 2613, 2642
BseGI	GGATG	8 cut(s)	348, 575, 808, 1099, 1118, 2407, 2636, 2719
BseJI	GATNNNNATC	1 cut(s)	2079
BseLI	CCNNNNNNNGG	5 cut(s)	323, 547, 803, 1457, 2269
BseMI	GCAATG	2 cut(s)	1962, 1968
BseMII	CTCAG	5 cut(s)	989, 1902, 2037, 2259, 2475
BseNI	ACTGG	5 cut(s)	775, 782, 1186, 1510, 2362
BseRI	GAGGAG	1 cut(s)	2318
BseSI	GKGCMC	2 cut(s)	113, 299
BseX3I	CGGCCG	1 cut(s)	131
BseYI	CCCAGC	2 cut(s)	49, 541
BsgI	GTGCAG	3 cut(s)	1361, 1637, 1839
Bsh1236I	CGCG	1 cut(s)	143
Bsh1285I	CGRYCG	1 cut(s)	134
BshFI	GGCC	9 cut(s)	77, 133, 147, 232, 297, 327, 1954, 2558, 2695
BshNI	GGYRCC	1 cut(s)	1102
BsiEI	CGRYCG	1 cut(s)	134
BsiHKAI	GWGCWC	2 cut(s)	164, 423
BsiHKCI	CYCGRG	1 cut(s)	1305
BsiSI	CCGG	2 cut(s)	893, 1032
BslFI	GGGAC	3 cut(s)	769, 788, 1386
BslI	CCNNNNNNNGG	5 cut(s)	323, 547, 803, 1457, 2269
BsmAI	GTCTC	2 cut(s)	1675, 2169
BsmFI	GGGAC	3 cut(s)	769, 788, 1386
BsmI	GAATGC	2 cut(s)	1509, 2244
BsnI	GGCC	9 cut(s)	77, 133, 147, 232, 297, 327, 1954, 2558, 2695
BsoBI	CYCGRG	1 cut(s)	1305
Bsp120I	GGGCCC	1 cut(s)	295
Bsp1286I	GDGCHC	4 cut(s)	113, 164, 299, 423
Bsp143I	GATC	7 cut(s)	212, 537, 550, 1111, 1336, 2074, 2598
Bsp19I	CCATGG	2 cut(s)	585, 1974
BspANI	GGCC	9 cut(s)	77, 133, 147, 232, 297, 327, 1954, 2558, 2695
BspCNI	CTCAG	5 cut(s)	988, 1903, 2038, 2260, 2476
BspFNI	CGCG	1 cut(s)	143
BspLI	GGNNCC	4 cut(s)	297, 470, 1104, 2094
BspMAI	CTGCAG	1 cut(s)	184
BspPI	GGATC	4 cut(s)	532, 558, 1106, 2606
BspQI	GCTCTTC	1 cut(s)	178
BspT107I	GGYRCC	1 cut(s)	1102
BsrBI	CCGCTC	1 cut(s)	2670
BsrDI	GCAATG	2 cut(s)	1962, 1968
BsrFI	RCCGGY	1 cut(s)	1031
BsrI	ACTGG	5 cut(s)	775, 782, 1186, 1510, 2362
BssAI	RCCGGY	1 cut(s)	1031
BssMI	GATC	7 cut(s)	212, 537, 550, 1111, 1336, 2074, 2598
BssNI	GRCGYC	1 cut(s)	1959
BssT1I	CCWWGG	4 cut(s)	530, 585, 1158, 1974
Bst2UI	CCWGG	5 cut(s)	329, 1124, 1293, 2613, 2642
Bst4CI	ACNGT	9 cut(s)	725, 821, 1525, 1668, 1860, 1906, 1943, 2058, 2435
Bst6I	CTCTTC	4 cut(s)	178, 1567, 2214, 2562
BstACI	GRCGYC	1 cut(s)	1959
BstAPI	GCANNNNNTGC	1 cut(s)	1489
BstBAI	YACGTR	1 cut(s)	1202
BstC8I	GCNNGC	3 cut(s)	145, 299, 353
BstDEI	CTNAG	9 cut(s)	641, 975, 1281, 1685, 1696, 1911, 2046, 2268, 2484
BstDSI	CCRYGG	3 cut(s)	141, 585, 1974
BstENI	CCTNNNNNAGG	1 cut(s)	2267
BstF5I	GGATG	8 cut(s)	348, 575, 808, 1099, 1118, 2407, 2636, 2719
BstFNI	CGCG	1 cut(s)	143
BstKTI	GATC	7 cut(s)	215, 540, 553, 1114, 1339, 2077, 2601
BstMAI	GTCTC	2 cut(s)	1675, 2169
BstMBI	GATC	7 cut(s)	212, 537, 550, 1111, 1336, 2074, 2598
BstMCI	CGRYCG	1 cut(s)	134
BstMWI	GCNNNNNNNGC	8 cut(s)	83, 117, 159, 168, 294, 1489, 1850, 1853
BstNI	CCWGG	5 cut(s)	329, 1124, 1293, 2613, 2642
BstNSI	RCATGY	1 cut(s)	355
BstSCI	CCNGG	6 cut(s)	327, 892, 1122, 1291, 2611, 2640
BstSFI	CTRYAG	3 cut(s)	180, 918, 1350
BstSLI	GKGCMC	2 cut(s)	113, 299
BstUI	CGCG	1 cut(s)	143
BstV2I	GAAGAC	2 cut(s)	255, 516
BstX2I	RGATCY	2 cut(s)	550, 1336
BstXI	CCANNNNNNTGG	1 cut(s)	1975
BstYI	RGATCY	2 cut(s)	550, 1336
BstZI	CGGCCG	1 cut(s)	131
Bsu36I	CCTNAGG	2 cut(s)	2268, 2484
BsuRI	GGCC	9 cut(s)	77, 133, 147, 232, 297, 327, 1954, 2558, 2695
BtgI	CCRYGG	3 cut(s)	141, 585, 1974
BtsCI	GGATG	8 cut(s)	348, 575, 808, 1099, 1118, 2407, 2636, 2719
BtsIMutI	CAGTG	6 cut(s)	1521, 1856, 1911, 1948, 2017, 2440
Cac8I	GCNNGC	3 cut(s)	145, 299, 353
CaiI	CAGNNNCTG	1 cut(s)	1910
Cfr10I	RCCGGY	1 cut(s)	1031
Cfr13I	GGNCC	9 cut(s)	76, 145, 230, 295, 296, 677, 2093, 2423, 2557
Cfr42I	CCGCGG	1 cut(s)	144
Csp6I	GTAC	4 cut(s)	884, 1577, 1653, 1939
CviQI	GTAC	4 cut(s)	884, 1577, 1653, 1939
DdeI	CTNAG	9 cut(s)	641, 975, 1281, 1685, 1696, 1911, 2046, 2268, 2484
DpnI	GATC	7 cut(s)	214, 539, 552, 1113, 1338, 2076, 2600
DpnII	GATC	7 cut(s)	212, 537, 550, 1111, 1336, 2074, 2598
EaeI	YGGCCR	2 cut(s)	131, 2693
EagI	CGGCCG	1 cut(s)	131
Eam1104I	CTCTTC	4 cut(s)	178, 1567, 2214, 2562
EarI	CTCTTC	4 cut(s)	178, 1567, 2214, 2562
Ecl136II	GAGCTC	1 cut(s)	421
EclXI	CGGCCG	1 cut(s)	131
Eco130I	CCWWGG	4 cut(s)	530, 585, 1158, 1974
Eco24I	GRGCYC	2 cut(s)	299, 423
Eco32I	GATATC	1 cut(s)	2574
Eco47I	GGWCC	3 cut(s)	677, 2093, 2423
Eco52I	CGGCCG	1 cut(s)	131
Eco53kI	GAGCTC	1 cut(s)	421
Eco57I	CTGAAG	1 cut(s)	629
Eco72I	CACGTG	1 cut(s)	1202
Eco81I	CCTNAGG	2 cut(s)	2268, 2484
Eco88I	CYCGRG	1 cut(s)	1305
EcoICRI	GAGCTC	1 cut(s)	421
EcoNI	CCTNNNNNAGG	1 cut(s)	2267
EcoO109I	RGGNCCY	2 cut(s)	230, 295
EcoRII	CCWGG	5 cut(s)	327, 1122, 1291, 2611, 2640
EcoRV	GATATC	1 cut(s)	2574
EcoT14I	CCWWGG	4 cut(s)	530, 585, 1158, 1974
EcoT38I	GRGCYC	2 cut(s)	299, 423
ErhI	CCWWGG	4 cut(s)	530, 585, 1158, 1974
FalI	AAGNNNNNCTT	4 cut(s)	1404, 1436, 1806, 1838
FaqI	GGGAC	3 cut(s)	769, 788, 1386
FauI	CCCGC	4 cut(s)	136, 306, 1993, 2505
FauNDI	CATATG	1 cut(s)	2071
FblI	GTMKAC	1 cut(s)	33
FokI	GGATG	8 cut(s)	355, 562, 815, 1105, 1106, 2394, 2623, 2726
FriOI	GRGCYC	2 cut(s)	299, 423
FspBI	CTAG	6 cut(s)	906, 1584, 2103, 2157, 2231, 2577
GsaI	CCCAGC	2 cut(s)	53, 545
HaeIII	GGCC	9 cut(s)	77, 133, 147, 232, 297, 327, 1954, 2558, 2695
HapII	CCGG	2 cut(s)	893, 1032
Hin1I	GRCGYC	1 cut(s)	1959
HincII	GTYRAC	2 cut(s)	2100, 2154
HindII	GTYRAC	2 cut(s)	2100, 2154
HindIII	AAGCTT	3 cut(s)	825, 1820, 2498
HpaII	CCGG	2 cut(s)	893, 1032
HphI	GGTGA	9 cut(s)	547, 715, 786, 882, 1021, 1138, 1585, 1649, 2372
Hpy166II	GTNNAC	3 cut(s)	34, 2100, 2154
Hpy8I	GTNNAC	3 cut(s)	34, 2100, 2154
Hpy99I	CGWCG	1 cut(s)	2708
HpyAV	CCTTC	9 cut(s)	221, 278, 604, 842, 891, 1084, 2249, 2460, 2539
HpyCH4III	ACNGT	9 cut(s)	725, 821, 1525, 1668, 1860, 1906, 1943, 2058, 2435
HpyCH4IV	ACGT	4 cut(s)	1201, 1263, 1743, 1959
HpyF10VI	GCNNNNNNNGC	8 cut(s)	83, 117, 159, 168, 294, 1489, 1850, 1853
HpyF3I	CTNAG	9 cut(s)	641, 975, 1281, 1685, 1696, 1911, 2046, 2268, 2484
HpySE526I	ACGT	4 cut(s)	1201, 1263, 1743, 1959
Hsp92I	GRCGYC	1 cut(s)	1959
KspI	CCGCGG	1 cut(s)	144
Kzo9I	GATC	7 cut(s)	212, 537, 550, 1111, 1336, 2074, 2598
LguI	GCTCTTC	1 cut(s)	178
LmnI	GCTCC	7 cut(s)	58, 167, 468, 589, 1159, 2305, 2717
LweI	GCATC	8 cut(s)	379, 581, 916, 1069, 1229, 1819, 2208, 2645
MaeI	CTAG	6 cut(s)	906, 1584, 2103, 2157, 2231, 2577
MaeII	ACGT	4 cut(s)	1201, 1263, 1743, 1959
MaeIII	GTNAC	7 cut(s)	703, 792, 853, 1174, 2052, 2344, 2360
MalI	GATC	7 cut(s)	214, 539, 552, 1113, 1338, 2076, 2600
MbiI	CCGCTC	1 cut(s)	2670
MboI	GATC	7 cut(s)	212, 537, 550, 1111, 1336, 2074, 2598
MfeI	CAATTG	2 cut(s)	318, 1107
MflI	RGATCY	2 cut(s)	550, 1336
MhlI	GDGCHC	4 cut(s)	113, 164, 299, 423
MlsI	TGGCCA	1 cut(s)	2695
MluNI	TGGCCA	1 cut(s)	2695
MlyI	GAGTC	7 cut(s)	245, 435, 800, 866, 2155, 2231, 2253
Mox20I	TGGCCA	1 cut(s)	2695
MroXI	GAANNNNTTC	1 cut(s)	1419
MscI	TGGCCA	1 cut(s)	2695
MseI	TTAA	5 cut(s)	258, 597, 1322, 1562, 2493
MslI	CAYNNNNRTG	4 cut(s)	504, 519, 1709, 1973
Msp20I	TGGCCA	1 cut(s)	2695
MspA1I	CMGCKG	3 cut(s)	143, 150, 950
MspI	CCGG	2 cut(s)	893, 1032
MspR9I	CCNGG	6 cut(s)	329, 894, 1124, 1293, 2613, 2642
MunI	CAATTG	2 cut(s)	318, 1107
Mva1269I	GAATGC	2 cut(s)	1509, 2244
MvaI	CCWGG	5 cut(s)	329, 1124, 1293, 2613, 2642
MvnI	CGCG	1 cut(s)	143
MwoI	GCNNNNNNNGC	8 cut(s)	83, 117, 159, 168, 294, 1489, 1850, 1853
NciI	CCSGG	1 cut(s)	894
NcoI	CCATGG	2 cut(s)	585, 1974
NdeI	CATATG	1 cut(s)	2071
NdeII	GATC	7 cut(s)	212, 537, 550, 1111, 1336, 2074, 2598
NlaIV	GGNNCC	4 cut(s)	297, 470, 1104, 2094
NmuCI	GTSAC	5 cut(s)	703, 792, 1174, 2052, 2360
NspI	RCATGY	1 cut(s)	355
OliI	CACNNNNGTG	1 cut(s)	519
PaeI	GCATGC	1 cut(s)	355
PciSI	GCTCTTC	1 cut(s)	178
PctI	GAATGC	2 cut(s)	1509, 2244
PdmI	GAANNNNTTC	1 cut(s)	1419
PfeI	GAWTC	7 cut(s)	911, 1145, 1301, 1689, 2276, 2318, 2729
PflMI	CCANNNNNTGG	2 cut(s)	323, 803
PleI	GAGTC	7 cut(s)	245, 434, 799, 866, 2154, 2230, 2253
PmaCI	CACGTG	1 cut(s)	1202
PmlI	CACGTG	1 cut(s)	1202
PpsI	GAGTC	7 cut(s)	245, 434, 799, 866, 2154, 2230, 2253
Ppu21I	YACGTR	1 cut(s)	1202
PsiI	TTATAA	2 cut(s)	968, 1529
Psp124BI	GAGCTC	1 cut(s)	423
Psp6I	CCWGG	5 cut(s)	327, 1122, 1291, 2611, 2640
PspCI	CACGTG	1 cut(s)	1202
PspFI	CCCAGC	2 cut(s)	49, 541
PspGI	CCWGG	5 cut(s)	327, 1122, 1291, 2611, 2640
PspN4I	GGNNCC	4 cut(s)	297, 470, 1104, 2094
PspOMI	GGGCCC	1 cut(s)	295
PspPI	GGNCC	9 cut(s)	76, 145, 230, 295, 296, 677, 2093, 2423, 2557
PstI	CTGCAG	1 cut(s)	184
PstNI	CAGNNNCTG	1 cut(s)	1910
PsuI	RGATCY	2 cut(s)	550, 1336
PvuII	CAGCTG	1 cut(s)	950
RsaI	GTAC	4 cut(s)	885, 1578, 1654, 1940
RsaNI	GTAC	4 cut(s)	884, 1577, 1653, 1939
RseI	CAYNNNNRTG	4 cut(s)	504, 519, 1709, 1973
SacI	GAGCTC	1 cut(s)	423
SacII	CCGCGG	1 cut(s)	144
SapI	GCTCTTC	1 cut(s)	178
SaqAI	TTAA	5 cut(s)	258, 597, 1322, 1562, 2493
Sau3AI	GATC	7 cut(s)	212, 537, 550, 1111, 1336, 2074, 2598
Sau96I	GGNCC	9 cut(s)	76, 145, 230, 295, 296, 677, 2093, 2423, 2557
ScaI	AGTACT	1 cut(s)	1578
SchI	GAGTC	7 cut(s)	245, 435, 800, 866, 2155, 2231, 2253
ScrFI	CCNGG	6 cut(s)	329, 894, 1124, 1293, 2613, 2642
SduI	GDGCHC	4 cut(s)	113, 164, 299, 423
SfaNI	GCATC	8 cut(s)	379, 581, 916, 1069, 1229, 1819, 2208, 2645
SfcI	CTRYAG	3 cut(s)	180, 918, 1350
Sfr303I	CCGCGG	1 cut(s)	144
SgrBI	CCGCGG	1 cut(s)	144
SinI	GGWCC	3 cut(s)	677, 2093, 2423
SmiMI	CAYNNNNRTG	4 cut(s)	504, 519, 1709, 1973
SmlI	CTYRAG	3 cut(s)	1063, 1188, 2373
SmoI	CTYRAG	3 cut(s)	1063, 1188, 2373
SphI	GCATGC	1 cut(s)	355
SspI	AATATT	1 cut(s)	567
SspMI	CTAG	6 cut(s)	906, 1584, 2103, 2157, 2231, 2577
SstI	GAGCTC	1 cut(s)	423
StyD4I	CCNGG	6 cut(s)	327, 892, 1122, 1291, 2611, 2640
StyI	CCWWGG	4 cut(s)	530, 585, 1158, 1974
TaaI	ACNGT	9 cut(s)	725, 821, 1525, 1668, 1860, 1906, 1943, 2058, 2435
TaiI	ACGT	4 cut(s)	1204, 1266, 1746, 1962
TaqI	TCGA	7 cut(s)	200, 235, 1674, 1795, 2077, 2253, 2703
TatI	WGTACW	3 cut(s)	883, 1576, 1938
TauI	GCSGC	4 cut(s)	133, 143, 150, 1438
TfiI	GAWTC	7 cut(s)	911, 1145, 1301, 1689, 2276, 2318, 2729
Tru1I	TTAA	5 cut(s)	258, 597, 1322, 1562, 2493
Tru9I	TTAA	5 cut(s)	258, 597, 1322, 1562, 2493
TscAI	CASTG	6 cut(s)	1528, 1863, 1911, 1948, 2017, 2440
TseFI	GTSAC	5 cut(s)	703, 792, 1174, 2052, 2360
Tsp45I	GTSAC	5 cut(s)	703, 792, 1174, 2052, 2360
TspDTI	ATGAA	9 cut(s)	297, 359, 522, 755, 1314, 1328, 1500, 1502, 2394
TspGWI	ACGGA	8 cut(s)	45, 96, 154, 296, 621, 1231, 2085, 2231
TspRI	CASTG	6 cut(s)	1528, 1863, 1911, 1948, 2017, 2440
Van91I	CCANNNNNTGG	2 cut(s)	323, 803
VpaK11BI	GGWCC	3 cut(s)	677, 2093, 2423
XagI	CCTNNNNNAGG	1 cut(s)	2267
XapI	RAATTY	2 cut(s)	665, 1725
XbaI	TCTAGA	1 cut(s)	2576
XceI	RCATGY	1 cut(s)	355
XmiI	GTMKAC	1 cut(s)	33
XmnI	GAANNNNTTC	1 cut(s)	1419
XspI	CTAG	6 cut(s)	906, 1584, 2103, 2157, 2231, 2577
ZraI	GACGTC	1 cut(s)	1960
ZrmI	AGTACT	1 cut(s)	1578

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.