Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0008506

Physical Location & Seq

Genomic Coordinates Reverse (-)

25307465 .. 25314890

Gene Structure 7426 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1158900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2754 bp

ATGCAATTAGGGGTTTTGGGGGGTTTATCTGTTTACCACTCTTTCTACCCAGCTCCGTTTAAACGAAGCGCGTCCGCCCAAATGGCCGCTCTCGGAGCTCTTTCTCCCTGTCCCTACAGCCTCCTGTGGCGGCCGAAAACCTCGAACCGCTGCGTTTCGTACTCCGTGGGCTCTTCTACTGCTACAGGAACACGTAGGTCGAATGTAAGAAGATCAGGAAGAATGGAAGGGCCTCGGAAAAGTATGGAAGACTCTGTTCAACGAAAGATGGAACAATTTTATGAAGGGAGAGAGGGCCCGCCAATCCGTGTTTTACCAATTGGTGGCCTTGGAGAAATTGGGATGAATTGCATGCTGGTCGGGAATTATGATCGCTACATTCTGATTGATGCTGGTGTCATGTTTCCGGACTTTGATGAGCTTGGAGTCCAAAAGATTATACCTGATACCACATTTATCAAAAAATGGAGCCACAAAATTGAAGCAATTGTTATTACACATGGGCACGAAGACCACATTGGTGCGTTGCCTTGGGTGATCCCAGCTTTGGATCCCAATACACCAATATTTGCATCATCCTTTACCATGGAGCTTATCAAAAAACGTCTGAAGGAACACGGGATCTTTGTTCCATCTAGACTTAGGACATTTAGAACAAAGAGGAAATTTACGGCTGGACCATTTGAGATTGAGCCTGTTCGGGTGACACATTCTATTCCTGACTGTTGTGGATTAGTTCTTCGCTGTTCTGATGGGACAATTCTTCACACTGGGGACTGGAAGATTGACGAATCACCATTGGATGGTAGAGGTTTTGACCGCGAAGCCTTGGAAGAACTTTCAAAAGAAGGAGTAACATTGATGATGAGTGACTCTACAAACGTACTCTCACCTGGAAGGACGACAAGTGAATCTTCCGTGGCAGATGCGCTGTTGAAGCATATTTCAGCTGCGAAAGGGAGGGTTATTACTACTCAGTTTGCTTCAAACATACACCGACTTGGAAGCGTGAAAGCTGCTGCTGATTTCACTGGCAGAAAGTTGGTATTTGTTGGTATGTCCTTAAGGACATATCTTGATGCAGCCTGGAAGGATGGGAAGGCACCAATTGATCCATCATCCTTGGTGAAAGTGGAAGATATTGATTCCTATGCCCCAAAGGATTTGCTGATTGTCACAACTGGCTCTCAAGCAGAACCACGTGCTGCTCTAAATCTTGCATCATTCGGAAGTAGTCATTCCGTCAAACTGAACAAAGAGGACATAATTTTGTATTCAGCTAAGGTAATTCCTGGTAATGAATCTAGGGTAATGAAAATGTTAAACCGCATATCAGATCTTGGGTCAACTATAGTGATGGGTAAGAATGAGGGGCTGCACACTTCTGGTCATGGGTATCGTGGAGAATTGGAAGAAGTCCTTCAAATTGTGAAGCCGCAACATTTTCTGCCCATACATGGAGAACTCTTGTTTCTCAAAGAGCATGAATTGCTTGGGAGATCAACTGGCATTCATCACACCACTGTTATAAAAAACGGGGAAATGCTTGGGGTTTCTCATTTAAGAAACAGAAGAGTACTTTCCAATGGTTTCACTTTGCTTGGAAAAGAGAATTTGCAGTTGAAGTTTAGCGACGGCGATAAAGCATTTGGCACATCAAGCGACCTTTGTGTTGACGAGAGACTAAGAATTGCCTTAGATGGCATAATAGTGGTCAGCATGGAAGTTTTACGCCCTCAAAATGTAAATGGTTCGACTGAAAATAGCATAAAAGGGAAGATAAAAATTACTACTCGATGTTTATGGCTTGACAAAGGAAAGCTTATTGATGCACTCTACAAAGCTGCCCATTCTGCACTATCAAGCTGCCCCATAAGTTGTCCTCTTCCTCACATGGAAAGAACAGTGTCTGAGGTATTGAGGAAATTGGTAAGAAAGTACAGTGGTAAAAGGCCAGACGTCATTGCCATTGCCATGGAGAACCCTGCAGCAGTTCATGCTGATGAGGTCAGTGCAAGACTATCTGGAAAATCTTATGTTGGTTCAGAGGTGTCACAGTTGAGGAAAGTATATGATCAACATCAAATTAAGAGTCCGTCAACTAGGACACAAGCAGATGATTGCAAGGCCAAAACACATTTTCCGAGTCAGTCAACTACAACACAAGCAGATGAAGGCAAGGACAACACATTGCAGAGCTTCTCTCAACAAGATACTGAAGAGTCCGTACTTGAAGATGATGGCATTGAAGTCGAAGGACTCCTGCCTGAGGAAGATTCTGCCACTTCAAGTTCCAAGCCAGAGAAGCTTTCTTCTGATTCAGAGGATTTTTTGAACGCAATGGTTAGATTATCAACTGGTGACAAATCAGTAGAAGATGAAAATGGTCTGGTTGCAGAACAGGAAAATCCACAGAAAGATGGTCCTGAAAGCAGTGAAATACCAAATCTTTCAAAGCCCGTGAAGCGCAATAAATGGAAGACTGACGAAGTTCAGAAGCTTATTACAATGCGGGGGAAACTGCGTAGCAGATTTCAAGTTGTGAAGGGTAGAATGGCCCTCTGGGAAGAGATATCTAGAAACTTGTTGGCCGATGGGATCAACCGAAGCCCAGGACAATGCAAATCTCTGTGGGCATCCCTGGTTCAGAAATATGAGGAAAGCAAGAGCGGAAAAAGAAGTCGAAAGAGTTGGCCATACTTTGAGGAAATGGATGCAGCTTTGTCTGATTCCGAGGAATTGGCAACAAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000578 GO:0003006 GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004527 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0006810 GO:0006996 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0008409 GO:0009314 GO:0009416 GO:0009507 GO:0009536 GO:0009628 GO:0009657 GO:0009658 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009880 GO:0009914 GO:0009942 GO:0009987 GO:0010154 GO:0010817 GO:0016043 GO:0016787 GO:0016788 GO:0022414 GO:0032501 GO:0032502 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0051179 GO:0051234 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Lactamase_B Lactamase_B_2 Myb_DNA-bind_4 RMMBL

Protein Analysis

918

Amino Acids

101.29

Weight (kDa)

8.36

Isoelectric Point (pI)

43.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lactamase_B	PF00753	116 - 259	6.5e-08	Metallo-beta-lactamase superfamily
Lactamase_B_2	PF12706	125 - 261	1.9e-10	Beta-lactamase superfamily domain
RNase_J_b_CASP	PF22505	319 - 444	3e-29	Ribonuclease J, beta-CASP domain
RMMBL	PF07521	459 - 505	1.8e-06	Zn-dependent metallo-hydrolase RNA specificity domain
Myb_DNA-bind_4	PF13837	823 - 906	2e-22	Myb/SANT-like DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0008506)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63420
fragaria_vesca	FvH4_5g07980 FvH4_5g07980 FvH4_5g07980 FvH4_5g07980
malus_domestica	MD06G1143600.v1.1 MD14G1158900.v1.1
prunus_persica	Prupe.5G152800_v2.0.a1 Prupe.5G152800_v2.0.a1 Prupe.5G152800_v2.0.a1
pyrus_communis	pycom06g13330 pycom14g13250
rosa_chinensis	RchiOBHm_Chr7g0190431
rosa_laevigata	RLG00000004533
rosa_multiflora	Rmu_ssc0000036.1_g000001
rosa_roxburghii	Rroxscaffold_3G00264630
rosa_rugosa	Rorug06G0504700 Rorug06G0504700
rosa_samantha	Rh7AG110500 Rh7BG113100 Rh7CG115300
rosa_wichuraiana	Rw0G019850 Rw7G009590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1529
AatII	GACGTC	1 cut(s)	1962
AccB1I	GGYRCC	1 cut(s)	1102
AccB7I	CCANNNNNTGG	2 cut(s)	323, 803
AccBSI	CCGCTC	2 cut(s)	89, 2670
AccII	CGCG	2 cut(s)	71, 822
AccIII	TCCGGA	1 cut(s)	406
AclWI	GGATC	6 cut(s)	532, 545, 558, 629, 1106, 2606
AcoI	YGGCCR	4 cut(s)	84, 131, 2589, 2693
AcsI	RAATTY	2 cut(s)	665, 1612
AcuI	CTGAAG	2 cut(s)	629, 2238
AcvI	CACGTG	1 cut(s)	1202
AcyI	GRCGYC	1 cut(s)	1959
AfaI	GTAC	5 cut(s)	161, 885, 1578, 1940, 2229
AfiI	CCNNNNNNNGG	5 cut(s)	323, 547, 803, 1457, 2269
AflII	CTTAAG	1 cut(s)	1063
AflIII	ACRYGT	1 cut(s)	191
AjnI	CCWGG	5 cut(s)	892, 1085, 1291, 2611, 2640
AjuI	GAANNNNNNNTTGG	2 cut(s)	501, 533
AleI	CACNNNNGTG	1 cut(s)	519
Alw21I	GWGCWC	1 cut(s)	100
Alw26I	GTCTC	1 cut(s)	1675
AlwI	GGATC	6 cut(s)	532, 545, 558, 629, 1106, 2606
AlwNI	CAGNNNCTG	1 cut(s)	1910
Aor13HI	TCCGGA	1 cut(s)	406
ApaI	GGGCCC	1 cut(s)	299
ApoI	RAATTY	2 cut(s)	665, 1612
ArsI	GACNNNNNNTTYG	2 cut(s)	2077, 2109
Asp700I	GAANNNNTTC	1 cut(s)	1419
AspLEI	GCGC	3 cut(s)	71, 931, 2469
AspS9I	GGNCC	6 cut(s)	230, 295, 296, 677, 2423, 2557
AsuHPI	GGTGA	6 cut(s)	547, 715, 786, 882, 1138, 2372
AvaII	GGWCC	2 cut(s)	677, 2423
AxyI	CCTNAGG	1 cut(s)	2268
BaeGI	GKGCMC	2 cut(s)	299, 507
BalI	TGGCCA	1 cut(s)	2695
BamHI	GGATCC	1 cut(s)	550
BanI	GGYRCC	1 cut(s)	1102
BanII	GRGCYC	3 cut(s)	100, 173, 299
BarI	GAAGNNNNNNTAC	2 cut(s)	2211, 2243
BbrPI	CACGTG	1 cut(s)	1202
BbsI	GAAGAC	3 cut(s)	255, 516, 2486
Bbv12I	GWGCWC	1 cut(s)	100
BceAI	ACGGC	2 cut(s)	687, 1651
BcgI	CGANNNNNNTGC	4 cut(s)	340, 374, 2233, 2267
BciT130I	CCWGG	5 cut(s)	894, 1087, 1293, 2613, 2642
BclI	TGATCA	1 cut(s)	2074
BcoDI	GTCTC	1 cut(s)	1675
BfaI	CTAG	4 cut(s)	636, 1305, 2103, 2577
BfmI	CTRYAG	4 cut(s)	115, 183, 1350, 1986
BfrI	CTTAAG	1 cut(s)	1063
BglI	GCCNNNNNGGC	1 cut(s)	83
BglII	AGATCT	1 cut(s)	1336
BmcAI	AGTACT	1 cut(s)	1578
Bme1390I	CCNGG	5 cut(s)	894, 1087, 1293, 2613, 2642
Bme18I	GGWCC	2 cut(s)	677, 2423
BmgT120I	GGNCC	6 cut(s)	230, 295, 296, 677, 2423, 2557
BmiI	GGNNCC	4 cut(s)	297, 470, 552, 1104
BmrFI	CCNGG	5 cut(s)	894, 1087, 1293, 2613, 2642
BmrI	ACTGGG	1 cut(s)	780
BmsI	GCATC	8 cut(s)	379, 581, 916, 1069, 1229, 1819, 2645, 2704
BmuI	ACTGGG	1 cut(s)	780
BpiI	GAAGAC	3 cut(s)	255, 516, 2486
BplI	GAGNNNNNCTC	2 cut(s)	2188, 2220
Bpu10I	CCTNAGC	1 cut(s)	1281
BpuEI	CTTGAG	1 cut(s)	1173
BsaAI	YACGTR	2 cut(s)	194, 1202
BsaBI	GATNNNNATC	1 cut(s)	2079
BsaHI	GRCGYC	1 cut(s)	1959
BsaWI	WCCGGW	1 cut(s)	406
Bsc4I	CCNNNNNNNGG	5 cut(s)	323, 547, 803, 1457, 2269
Bse1I	ACTGG	6 cut(s)	775, 782, 1036, 1186, 1510, 2362
Bse21I	CCTNAGG	1 cut(s)	2268
Bse3DI	GCAATG	4 cut(s)	1962, 1968, 2189, 2346
Bse8I	GATNNNNATC	1 cut(s)	2079
BseAI	TCCGGA	1 cut(s)	406
BseBI	CCWGG	5 cut(s)	894, 1087, 1293, 2613, 2642
BseGI	GGATG	7 cut(s)	348, 575, 808, 1099, 1118, 2636, 2719
BseJI	GATNNNNATC	1 cut(s)	2079
BseLI	CCNNNNNNNGG	5 cut(s)	323, 547, 803, 1457, 2269
BseMI	GCAATG	4 cut(s)	1962, 1968, 2189, 2346
BseMII	CTCAG	3 cut(s)	989, 1902, 2259
BseNI	ACTGG	6 cut(s)	775, 782, 1036, 1186, 1510, 2362
BseSI	GKGCMC	2 cut(s)	299, 507
BseX3I	CGGCCG	1 cut(s)	131
BseYI	CCCAGC	2 cut(s)	49, 541
BsgI	GTGCAG	2 cut(s)	1361, 1839
Bsh1236I	CGCG	2 cut(s)	71, 822
Bsh1285I	CGRYCG	1 cut(s)	134
BshNI	GGYRCC	1 cut(s)	1102
BsiEI	CGRYCG	1 cut(s)	134
BsiHKAI	GWGCWC	1 cut(s)	100
BsiSI	CCGG	1 cut(s)	407
BslFI	GGGAC	3 cut(s)	96, 769, 788
BslI	CCNNNNNNNGG	5 cut(s)	323, 547, 803, 1457, 2269
BsmAI	GTCTC	1 cut(s)	1675
BsmFI	GGGAC	3 cut(s)	96, 769, 788
BsmI	GAATGC	1 cut(s)	1509
Bsp120I	GGGCCC	1 cut(s)	295
Bsp1286I	GDGCHC	4 cut(s)	100, 173, 299, 507
Bsp13I	TCCGGA	1 cut(s)	406
Bsp19I	CCATGG	2 cut(s)	585, 1974
BspCNI	CTCAG	3 cut(s)	988, 1903, 2260
BspEI	TCCGGA	1 cut(s)	406
BspFNI	CGCG	2 cut(s)	71, 822
BspLI	GGNNCC	4 cut(s)	297, 470, 552, 1104
BspMAI	CTGCAG	1 cut(s)	1990
BspPI	GGATC	6 cut(s)	532, 545, 558, 629, 1106, 2606
BspQI	GCTCTTC	1 cut(s)	178
BspT107I	GGYRCC	1 cut(s)	1102
BspTI	CTTAAG	1 cut(s)	1063
BsrBI	CCGCTC	2 cut(s)	89, 2670
BsrDI	GCAATG	4 cut(s)	1962, 1968, 2189, 2346
BsrI	ACTGG	6 cut(s)	775, 782, 1036, 1186, 1510, 2362
BssNI	GRCGYC	1 cut(s)	1959
BssT1I	CCWWGG	6 cut(s)	328, 530, 585, 828, 1122, 1974
Bst2UI	CCWGG	5 cut(s)	894, 1087, 1293, 2613, 2642
Bst4CI	ACNGT	5 cut(s)	725, 1525, 1906, 1943, 2058
Bst6I	CTCTTC	5 cut(s)	178, 1567, 1890, 2214, 2562
BstACI	GRCGYC	1 cut(s)	1959
BstAFI	CTTAAG	1 cut(s)	1063
BstAPI	GCANNNNNTGC	2 cut(s)	1489, 1997
BstBAI	YACGTR	2 cut(s)	194, 1202
BstC8I	GCNNGC	2 cut(s)	299, 353
BstDEI	CTNAG	7 cut(s)	641, 975, 1281, 1685, 1696, 1911, 2268
BstDSI	CCRYGG	4 cut(s)	165, 585, 918, 1974
BstENI	CCTNNNNNAGG	1 cut(s)	2267
BstF5I	GGATG	7 cut(s)	348, 575, 808, 1099, 1118, 2636, 2719
BstFNI	CGCG	2 cut(s)	71, 822
BstHHI	GCGC	3 cut(s)	71, 931, 2469
BstMAI	GTCTC	1 cut(s)	1675
BstMCI	CGRYCG	1 cut(s)	134
BstMWI	GCNNNNNNNGC	9 cut(s)	83, 95, 937, 1489, 1659, 1853, 1997, 2305, 2464
BstNI	CCWGG	5 cut(s)	894, 1087, 1293, 2613, 2642
BstNSI	RCATGY	1 cut(s)	355
BstSCI	CCNGG	5 cut(s)	892, 1085, 1291, 2611, 2640
BstSFI	CTRYAG	4 cut(s)	115, 183, 1350, 1986
BstSLI	GKGCMC	2 cut(s)	299, 507
BstUI	CGCG	2 cut(s)	71, 822
BstV2I	GAAGAC	3 cut(s)	255, 516, 2486
BstX2I	RGATCY	3 cut(s)	550, 621, 1336
BstXI	CCANNNNNNTGG	1 cut(s)	1975
BstYI	RGATCY	3 cut(s)	550, 621, 1336
BstZI	CGGCCG	1 cut(s)	131
Bsu36I	CCTNAGG	1 cut(s)	2268
BtgI	CCRYGG	4 cut(s)	165, 585, 918, 1974
BtsCI	GGATG	7 cut(s)	348, 575, 808, 1099, 1118, 2636, 2719
BtsI	GCAGTG	1 cut(s)	2440
BtsIMutI	CAGTG	7 cut(s)	768, 1029, 1521, 1911, 1948, 2017, 2440
Cac8I	GCNNGC	2 cut(s)	299, 353
CaiI	CAGNNNCTG	1 cut(s)	1910
CfoI	GCGC	3 cut(s)	71, 931, 2469
Cfr13I	GGNCC	6 cut(s)	230, 295, 296, 677, 2423, 2557
CseI	GACGC	1 cut(s)	60
Csp6I	GTAC	5 cut(s)	160, 884, 1577, 1939, 2228
CviQI	GTAC	5 cut(s)	160, 884, 1577, 1939, 2228
DdeI	CTNAG	7 cut(s)	641, 975, 1281, 1685, 1696, 1911, 2268
DraI	TTTAAA	1 cut(s)	61
EaeI	YGGCCR	4 cut(s)	84, 131, 2589, 2693
EagI	CGGCCG	1 cut(s)	131
Eam1104I	CTCTTC	5 cut(s)	178, 1567, 1890, 2214, 2562
EarI	CTCTTC	5 cut(s)	178, 1567, 1890, 2214, 2562
EciI	GGCGGA	1 cut(s)	64
Ecl136II	GAGCTC	1 cut(s)	98
EclXI	CGGCCG	1 cut(s)	131
Eco130I	CCWWGG	6 cut(s)	328, 530, 585, 828, 1122, 1974
Eco24I	GRGCYC	3 cut(s)	100, 173, 299
Eco32I	GATATC	1 cut(s)	2574
Eco47I	GGWCC	2 cut(s)	677, 2423
Eco52I	CGGCCG	1 cut(s)	131
Eco53kI	GAGCTC	1 cut(s)	98
Eco57I	CTGAAG	2 cut(s)	629, 2238
Eco72I	CACGTG	1 cut(s)	1202
Eco81I	CCTNAGG	1 cut(s)	2268
EcoICRI	GAGCTC	1 cut(s)	98
EcoNI	CCTNNNNNAGG	1 cut(s)	2267
EcoO109I	RGGNCCY	2 cut(s)	230, 295
EcoRII	CCWGG	5 cut(s)	892, 1085, 1291, 2611, 2640
EcoRV	GATATC	1 cut(s)	2574
EcoT14I	CCWWGG	6 cut(s)	328, 530, 585, 828, 1122, 1974
EcoT38I	GRGCYC	3 cut(s)	100, 173, 299
ErhI	CCWWGG	6 cut(s)	328, 530, 585, 828, 1122, 1974
FalI	AAGNNNNNCTT	6 cut(s)	898, 930, 1404, 1436, 1806, 1838
FaqI	GGGAC	3 cut(s)	96, 769, 788
FauI	CCCGC	2 cut(s)	306, 2505
FbaI	TGATCA	1 cut(s)	2074
FokI	GGATG	7 cut(s)	355, 562, 815, 1105, 1106, 2623, 2726
FriOI	GRGCYC	3 cut(s)	100, 173, 299
FspBI	CTAG	4 cut(s)	636, 1305, 2103, 2577
GlaI	GCGC	3 cut(s)	70, 930, 2468
GsaI	CCCAGC	2 cut(s)	53, 545
HapII	CCGG	1 cut(s)	407
HgaI	GACGC	1 cut(s)	60
HhaI	GCGC	3 cut(s)	71, 931, 2469
Hin1I	GRCGYC	1 cut(s)	1959
Hin6I	GCGC	3 cut(s)	69, 929, 2467
HinP1I	GCGC	3 cut(s)	69, 929, 2467
HincII	GTYRAC	4 cut(s)	1347, 1675, 2100, 2154
HindII	GTYRAC	4 cut(s)	1347, 1675, 2100, 2154
HindIII	AAGCTT	3 cut(s)	1820, 2306, 2498
HpaII	CCGG	1 cut(s)	407
HphI	GGTGA	6 cut(s)	547, 715, 786, 882, 1138, 2372
Hpy166II	GTNNAC	5 cut(s)	34, 1347, 1675, 2100, 2154
Hpy188III	TCNNGA	9 cut(s)	216, 361, 407, 636, 719, 1076, 2025, 2426, 2577
Hpy8I	GTNNAC	5 cut(s)	34, 1347, 1675, 2100, 2154
Hpy99I	CGWCG	1 cut(s)	1637
HpyCH4III	ACNGT	5 cut(s)	725, 1525, 1906, 1943, 2058
HpyCH4IV	ACGT	5 cut(s)	193, 604, 882, 1201, 1959
HpyF10VI	GCNNNNNNNGC	9 cut(s)	83, 95, 937, 1489, 1659, 1853, 1997, 2305, 2464
HpyF3I	CTNAG	7 cut(s)	641, 975, 1281, 1685, 1696, 1911, 2268
HpySE526I	ACGT	5 cut(s)	193, 604, 882, 1201, 1959
Hsp92I	GRCGYC	1 cut(s)	1959
HspAI	GCGC	3 cut(s)	69, 929, 2467
Kpn2I	TCCGGA	1 cut(s)	406
Ksp22I	TGATCA	1 cut(s)	2074
LguI	GCTCTTC	1 cut(s)	178
LmnI	GCTCC	4 cut(s)	58, 95, 468, 589
LweI	GCATC	8 cut(s)	379, 581, 916, 1069, 1229, 1819, 2645, 2704
MaeI	CTAG	4 cut(s)	636, 1305, 2103, 2577
MaeII	ACGT	5 cut(s)	193, 604, 882, 1201, 1959
MaeIII	GTNAC	6 cut(s)	703, 853, 869, 1174, 2052, 2360
MbiI	CCGCTC	2 cut(s)	89, 2670
MfeI	CAATTG	3 cut(s)	318, 486, 1107
MflI	RGATCY	3 cut(s)	550, 621, 1336
MhlI	GDGCHC	4 cut(s)	100, 173, 299, 507
MlsI	TGGCCA	1 cut(s)	2695
MluNI	TGGCCA	1 cut(s)	2695
MlyI	GAGTC	7 cut(s)	245, 435, 866, 2101, 2155, 2231, 2253
Mox20I	TGGCCA	1 cut(s)	2695
MroI	TCCGGA	1 cut(s)	406
MroXI	GAANNNNTTC	1 cut(s)	1419
MscI	TGGCCA	1 cut(s)	2695
MseI	TTAA	5 cut(s)	60, 1064, 1322, 1562, 2088
MslI	CAYNNNNRTG	4 cut(s)	519, 1709, 1973, 2001
Msp20I	TGGCCA	1 cut(s)	2695
MspA1I	CMGCKG	2 cut(s)	150, 950
MspCI	CTTAAG	1 cut(s)	1063
MspI	CCGG	1 cut(s)	407
MspR9I	CCNGG	5 cut(s)	894, 1087, 1293, 2613, 2642
MssI	GTTTAAAC	1 cut(s)	61
MunI	CAATTG	3 cut(s)	318, 486, 1107
Mva1269I	GAATGC	1 cut(s)	1509
MvaI	CCWGG	5 cut(s)	894, 1087, 1293, 2613, 2642
MvnI	CGCG	2 cut(s)	71, 822
MwoI	GCNNNNNNNGC	9 cut(s)	83, 95, 937, 1489, 1659, 1853, 1997, 2305, 2464
NcoI	CCATGG	2 cut(s)	585, 1974
NlaIV	GGNNCC	4 cut(s)	297, 470, 552, 1104
NmuCI	GTSAC	5 cut(s)	703, 869, 1174, 2052, 2360
NspI	RCATGY	1 cut(s)	355
OliI	CACNNNNGTG	1 cut(s)	519
PaeI	GCATGC	1 cut(s)	355
PciSI	GCTCTTC	1 cut(s)	178
PctI	GAATGC	1 cut(s)	1509
PdmI	GAANNNNTTC	1 cut(s)	1419
PfeI	GAWTC	7 cut(s)	791, 911, 1145, 1301, 2276, 2318, 2729
PflMI	CCANNNNNTGG	2 cut(s)	323, 803
PleI	GAGTC	7 cut(s)	245, 434, 866, 2100, 2154, 2230, 2253
PmaCI	CACGTG	1 cut(s)	1202
PmeI	GTTTAAAC	1 cut(s)	61
PmlI	CACGTG	1 cut(s)	1202
PpsI	GAGTC	7 cut(s)	245, 434, 866, 2100, 2154, 2230, 2253
Ppu21I	YACGTR	2 cut(s)	194, 1202
PsiI	TTATAA	1 cut(s)	1529
Psp124BI	GAGCTC	1 cut(s)	100
Psp6I	CCWGG	5 cut(s)	892, 1085, 1291, 2611, 2640
PspCI	CACGTG	1 cut(s)	1202
PspFI	CCCAGC	2 cut(s)	49, 541
PspGI	CCWGG	5 cut(s)	892, 1085, 1291, 2611, 2640
PspN4I	GGNNCC	4 cut(s)	297, 470, 552, 1104
PspOMI	GGGCCC	1 cut(s)	295
PspPI	GGNCC	6 cut(s)	230, 295, 296, 677, 2423, 2557
PstI	CTGCAG	1 cut(s)	1990
PstNI	CAGNNNCTG	1 cut(s)	1910
PsuI	RGATCY	3 cut(s)	550, 621, 1336
PvuII	CAGCTG	1 cut(s)	950
RsaI	GTAC	5 cut(s)	161, 885, 1578, 1940, 2229
RsaNI	GTAC	5 cut(s)	160, 884, 1577, 1939, 2228
RseI	CAYNNNNRTG	4 cut(s)	519, 1709, 1973, 2001
SacI	GAGCTC	1 cut(s)	100
SapI	GCTCTTC	1 cut(s)	178
SaqAI	TTAA	5 cut(s)	60, 1064, 1322, 1562, 2088
Sau96I	GGNCC	6 cut(s)	230, 295, 296, 677, 2423, 2557
ScaI	AGTACT	1 cut(s)	1578
SchI	GAGTC	7 cut(s)	245, 435, 866, 2101, 2155, 2231, 2253
ScrFI	CCNGG	5 cut(s)	894, 1087, 1293, 2613, 2642
SduI	GDGCHC	4 cut(s)	100, 173, 299, 507
SfaNI	GCATC	8 cut(s)	379, 581, 916, 1069, 1229, 1819, 2645, 2704
SfcI	CTRYAG	4 cut(s)	115, 183, 1350, 1986
SinI	GGWCC	2 cut(s)	677, 2423
SmiMI	CAYNNNNRTG	4 cut(s)	519, 1709, 1973, 2001
SmlI	CTYRAG	2 cut(s)	1063, 1188
SmoI	CTYRAG	2 cut(s)	1063, 1188
SphI	GCATGC	1 cut(s)	355
SspI	AATATT	1 cut(s)	567
SspMI	CTAG	4 cut(s)	636, 1305, 2103, 2577
SstI	GAGCTC	1 cut(s)	100
StyD4I	CCNGG	5 cut(s)	892, 1085, 1291, 2611, 2640
StyI	CCWWGG	6 cut(s)	328, 530, 585, 828, 1122, 1974
TaaI	ACNGT	5 cut(s)	725, 1525, 1906, 1943, 2058
TaiI	ACGT	5 cut(s)	196, 607, 885, 1204, 1962
TaqI	TCGA	6 cut(s)	143, 200, 1754, 1795, 2253, 2683
TatI	WGTACW	2 cut(s)	1576, 1938
TauI	GCSGC	3 cut(s)	89, 133, 1438
TfiI	GAWTC	7 cut(s)	791, 911, 1145, 1301, 2276, 2318, 2729
Tru1I	TTAA	5 cut(s)	60, 1064, 1322, 1562, 2088
Tru9I	TTAA	5 cut(s)	60, 1064, 1322, 1562, 2088
TscAI	CASTG	7 cut(s)	775, 1036, 1528, 1911, 1948, 2017, 2440
TseFI	GTSAC	5 cut(s)	703, 869, 1174, 2052, 2360
Tsp45I	GTSAC	5 cut(s)	703, 869, 1174, 2052, 2360
TspDTI	ATGAA	9 cut(s)	297, 359, 1314, 1328, 1500, 1502, 1985, 2187, 2394
TspGWI	ACGGA	7 cut(s)	45, 154, 296, 907, 1231, 2085, 2215
TspRI	CASTG	7 cut(s)	775, 1036, 1528, 1911, 1948, 2017, 2440
Van91I	CCANNNNNTGG	2 cut(s)	323, 803
Vha464I	CTTAAG	1 cut(s)	1063
VpaK11BI	GGWCC	2 cut(s)	677, 2423
XagI	CCTNNNNNAGG	1 cut(s)	2267
XapI	RAATTY	2 cut(s)	665, 1612
XbaI	TCTAGA	2 cut(s)	635, 2576
XceI	RCATGY	1 cut(s)	355
XmnI	GAANNNNTTC	1 cut(s)	1419
XspI	CTAG	4 cut(s)	636, 1305, 2103, 2577
ZraI	GACGTC	1 cut(s)	1960
ZrmI	AGTACT	1 cut(s)	1578

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.