Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0007701

Physical Location & Seq

Genomic Coordinates Forward (+)

25537074 .. 25541529

Gene Structure 4456 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G63810.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2226 bp

ATGAATCGGGTTACTACTGAAAGCATTGCGTCTACGGCGATACTGGTGGTGATGGTGTTTCTCTTCTCGTGGAGATCTATCGAGGCGGCGAATGTATCCTACGACCACCGTTCTCTCACCATCGGCAATCGCCGTCAGCTCATTATCTCCGCCGCCATTCACTATCCCAGAAGCGTCCCTGCTATGTGGCCATCTCTTGTTCAAACAGCAAAAGAAGGAGGCTGCAACGCAATCGAGTCTTATGTCTTCTGGAATGGCCATGAGCCATCTCCTGGAAAGTATTATTTTGGTGGCCGGTATAATATAGTGAAGTTTATCAAGATTGTTCAACAAGCTGGAATGCATATGATTCTACGAATTGGTCCTTTTGTTGCCGCCGAGTGGAATTACGGGGGAGTCCCGGTTTGGCTGCATTACGTGCCAGGAACAGTCTTCAGAGCGGATAATGAGCCATGGAAGCATTACATGGAGAGTTTCACGACGTATATAGTAAACCTGTTGAAACAAGAGAAGCTTTTTGCACCTCAGGGCGGTCCAATAATCTTGTCTCAGGTGGAAAACGAGTACGGGTATTATGAAAAGGATTACGGAGAAGGAGGGAAAAGGTATGCTCAATGGTCTGCTTCTATGGCTGTTTCACAGAATATTGGTGTTCCATGGATGATGTGCCAGCAATGGGATGCTCCTCCCACTGTGATTAGTACCTGCAATGGCTTTTACTGTGACCAATTCACACCTAATACACCAGATAAACCCAAGATTTGGACTGAGAACTGGCCTGGATGGTTTAAAACTTTTGGAGGCAGAGACCCTCACAGACCAGCAGAGGATGTAGCCTACTCTGTTGCTCGGTTTTTCGGGAAAGGTGGAAGTGTTCACAACTACTACATGTATCACGGAGGAACTAATTTTGGACGTACTTCAGGAGGACCTTTTATCACTACAAGCTATGACTATGAAGCTCCCATCGATGAGTATGGATTGCCTAGATTACCAAAATGGGGACACCTTAAGGATCTCCACAAAGCTATAATGCTCTCTGAAAACTTGCTGATAAGTGGCGAGCATCAAAATTTTACATTAGGCCATTCACTCGAGGCTGATGTGTACACAGACTCTTCGGGAACTTGTGCTGCGTTTCTGTCAAACTTGGATGATAAAAATGACAAGGCGGTGATGTTTCGAAACACATCATACCACTTGCCTGCTTGGTCAGTTAGCATTCTACCTGACTGCAAGACCGAGGTTTTCAACACAGCAAAGGTAACTTCGAAGTCCTCCAAGGTTGAAATGCTACCTGAAGATTTGAAATCTTCATCAGGTCTTAAATGGGAAGTATTCTCGGAGAAACCGGGTATTTGGGGCGCAGCAGATTTTGTGAAAAATGAACTTGTTGACCACATCAACACCACAAAGGACACAACTGACTATCTTTGGTACACAACAAGTATAACAGTCAGTGAAAATGAAGCGTTCTTGAAGAAAGGAAGCTCCCCGGTTCTGTTTATTGAATCAAAAGGGCATACTCTTCATGTTTTCATCAACAAAGAGTACTTAGGAACTGCGACAGGGAATGGGACTCATGTGCCTTTCAAACTCAAGAAACCAGTTGCTCTCAAAGCGGGCGAAAACAATATTGATTTGCTTAGCATGACTGTTGGTCTAGCGAATGCAGGATCCTTTTACGAATGGGTTGGAGCTGGACTTACAAGCGTCTCAATCAAAGGTTTCAACAAGGGCACATTGAATTTGACAAACAGCAAATGGTCCTACAAGCTCGGCGTGGAAGGAGAGCATCTAGAACTGTTCAAGCCAGGTAACTCCGGGGCTGTGAAATGGACGGTGACTACGAAACCTCCCAAGAAACAGCCTCTGACTTGGTATAAGGTTGTGATAGAGCCACCTTCTGGGAGTGAGCCGGTTGGGCTTGATATGATCAGTATGGGGAAAGGAATGGCATGGTTAAATGGTGAAGAGATAGGAAGATATTGGCCCAGGATTGCTAGAAAGAACAGCCCGAACGACGAGTGCGTAAAAGAATGCGATTACAGAGGCAAATTCATGCCGGATAAATGCCTAACTGGATGTGGAGAGCCATCTCAGAGATGGTATCATGTTCCAAGATCATGGTTTAAGTCTTCCGGGAATGAGCTGGTGATCTTCGAGGAGAAAGGCGGCAATCCCATGAAGATAAAGTTGTCCAAAAGGAAAGTATCGGTGGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0008150 GO:0009505 GO:0009628 GO:0015925 GO:0016787 GO:0016798 GO:0030312 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0071944 GO:0080167

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Gal_Lectin Glyco_hydro_35

Protein Analysis

741

Amino Acids

83.1

Weight (kDa)

8.58

Isoelectric Point (pI)

39.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	38 - 343	3.9e-114	Glycosyl hydrolases family 35
GHD	PF17834	366 - 422	1.1e-24	Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd	PF21467	625 - 716	1.4e-21	Beta-galactosidase, galactose-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007701)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63810
fragaria_vesca	FvH4_1g25130 FvH4_1g25130 FvH4_1g25130 FvH4_1g25130 FvH4_1g25130
malus_domestica	MD15G1136400.v1.1
prunus_persica	Prupe.4G278500_v2.0.a1 Prupe.4G278500_v2.0.a1 Prupe.4G278500_v2.0.a1
pyrus_communis	pycom15g12300
rosa_chinensis	RchiOBHm_Chr2g0122791
rosa_laevigata	RLG00000018715
rosa_multiflora	Rmu_sc0008528.1_g000013 Rmu_sc0037008.1_g000001
rosa_roxburghii	Rroxscaffold_2G00121170
rosa_rugosa	Rorug02G0242600 Rorug02G0242700 Rorug02G0242700
rosa_samantha	Rh2AG301300 Rh2BG308700 Rh2CG287400 Rh2DG325500
rosa_wichuraiana	Rw2G023890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	713
AccB7I	CCANNNNNTGG	3 cut(s)	272, 762, 1907
AccBSI	CCGCTC	1 cut(s)	440
AccI	GTMKAC	1 cut(s)	32
AciI	CCGC	9 cut(s)	86, 150, 153, 375, 440, 531, 1172, 1622, 2175
AclWI	GGATC	3 cut(s)	1023, 1671, 1684
AcoI	YGGCCR	3 cut(s)	188, 256, 292
AcsI	RAATTY	3 cut(s)	1072, 1747, 2057
AcuI	CTGAAG	3 cut(s)	418, 906, 1320
AfaI	GTAC	6 cut(s)	566, 703, 919, 1109, 1439, 1553
AfiI	CCNNNNNNNGG	7 cut(s)	272, 381, 530, 676, 762, 1001, 1907
AflII	CTTAAG	1 cut(s)	1010
AflIII	ACRYGT	1 cut(s)	888
AhdI	GACNNNNNGTC	1 cut(s)	1659
AjnI	CCWGG	5 cut(s)	271, 421, 778, 1813, 1994
Alw26I	GTCTC	3 cut(s)	552, 801, 1720
AlwI	GGATC	3 cut(s)	1023, 1671, 1684
AlwNI	CAGNNNCTG	1 cut(s)	1873
Ama87I	CYCGRG	1 cut(s)	1094
AoxI	GGCC	6 cut(s)	188, 256, 292, 776, 1084, 1991
ApeKI	GCWGC	4 cut(s)	222, 409, 1133, 1367
ApoI	RAATTY	3 cut(s)	1072, 1747, 2057
AspLEI	GCGC	1 cut(s)	1367
AspS9I	GGNCC	5 cut(s)	362, 533, 929, 1767, 1992
AsuC2I	CCSGG	5 cut(s)	401, 1353, 1496, 1825, 2143
AsuHPI	GGTGA	6 cut(s)	61, 109, 1186, 1855, 1982, 2167
AsuII	TTCGAA	2 cut(s)	1183, 1271
AvaI	CYCGRG	1 cut(s)	1094
AvaII	GGWCC	4 cut(s)	362, 533, 929, 1767
AxyI	CCTNAGG	1 cut(s)	525
BaeGI	GKGCMC	1 cut(s)	1742
BalI	TGGCCA	2 cut(s)	190, 258
BamHI	GGATCC	1 cut(s)	1676
BauI	CACGAG	1 cut(s)	67
BbsI	GAAGAC	3 cut(s)	238, 424, 2130
BbvI	GCAGC	4 cut(s)	209, 396, 1120, 1379
BccI	CCATC	8 cut(s)	46, 128, 199, 274, 777, 974, 2100, 2104
BceAI	ACGGC	2 cut(s)	51, 117
BcgI	CGANNNNNNTGC	2 cut(s)	214, 248
BciT130I	CCWGG	5 cut(s)	273, 423, 780, 1815, 1996
BciVI	GTATCC	1 cut(s)	106
BclI	TGATCA	1 cut(s)	1935
BcnI	CCSGG	5 cut(s)	401, 1353, 1496, 1825, 2143
BcoDI	GTCTC	3 cut(s)	552, 801, 1720
BfaI	CTAG	4 cut(s)	987, 1664, 1799, 2004
BfrI	CTTAAG	1 cut(s)	1010
BfuAI	ACCTGC	1 cut(s)	713
BfuI	GTATCC	1 cut(s)	106
BglI	GCCNNNNNGGC	1 cut(s)	1924
BglII	AGATCT	1 cut(s)	74
BisI	GCNGC	8 cut(s)	87, 153, 223, 375, 410, 1134, 1368, 2176
BlpI	GCTNAGC	1 cut(s)	1646
BlsI	GCNGC	8 cut(s)	88, 154, 224, 376, 411, 1135, 1369, 2177
BmcAI	AGTACT	1 cut(s)	1553
Bme18I	GGWCC	4 cut(s)	362, 533, 929, 1767
BmeRI	GACNNNNNGTC	1 cut(s)	1659
BmeT110I	CYCGRG	1 cut(s)	1094
BmgT120I	GGNCC	5 cut(s)	362, 533, 929, 1767, 1992
BmiI	GGNNCC	1 cut(s)	1678
BmsI	GCATC	3 cut(s)	670, 1075, 1804
BpiI	GAAGAC	3 cut(s)	238, 424, 2130
Bpu1102I	GCTNAGC	1 cut(s)	1646
Bpu14I	TTCGAA	2 cut(s)	1183, 1271
BpuEI	CTTGAG	1 cut(s)	1583
BpuMI	CCSGG	5 cut(s)	401, 1353, 1496, 1825, 2143
Bsa29I	ATCGAT	1 cut(s)	969
BsaAI	YACGTR	1 cut(s)	418
BsaI	GGTCTC	1 cut(s)	801
BsaJI	CCNNGG	7 cut(s)	452, 656, 1242, 1281, 1494, 1824, 1994
BsaXI	ACNNNNNCTCC	4 cut(s)	387, 417, 1840, 1870
Bsc4I	CCNNNNNNNGG	7 cut(s)	272, 381, 530, 676, 762, 1001, 1907
Bse118I	RCCGGY	2 cut(s)	294, 1918
Bse1I	ACTGG	4 cut(s)	48, 779, 1607, 2086
Bse21I	CCTNAGG	1 cut(s)	525
Bse3DI	GCAATG	3 cut(s)	24, 680, 715
BseBI	CCWGG	5 cut(s)	273, 423, 780, 1815, 1996
BseCI	ATCGAT	1 cut(s)	969
BseDI	CCNNGG	7 cut(s)	452, 656, 1242, 1281, 1494, 1824, 1994
BseGI	GGATG	6 cut(s)	666, 685, 788, 835, 1159, 2090
BseLI	CCNNNNNNNGG	7 cut(s)	272, 381, 530, 676, 762, 1001, 1907
BseMI	GCAATG	3 cut(s)	24, 680, 715
BseMII	CTCAG	4 cut(s)	539, 563, 759, 2114
BseNI	ACTGG	4 cut(s)	48, 779, 1607, 2086
BseRI	GAGGAG	2 cut(s)	675, 2180
BseSI	GKGCMC	1 cut(s)	1742
BseXI	GCAGC	4 cut(s)	209, 396, 1120, 1379
BshFI	GGCC	6 cut(s)	190, 258, 294, 778, 1086, 1993
BshVI	ATCGAT	1 cut(s)	969
BsiHKCI	CYCGRG	1 cut(s)	1094
BsiSI	CCGG	8 cut(s)	295, 401, 1352, 1496, 1824, 1919, 2066, 2142
BslFI	GGGAC	4 cut(s)	161, 383, 1017, 1591
BslI	CCNNNNNNNGG	7 cut(s)	272, 381, 530, 676, 762, 1001, 1907
BsmAI	GTCTC	3 cut(s)	552, 801, 1720
BsmBI	CGTCTC	1 cut(s)	1720
BsmFI	GGGAC	4 cut(s)	161, 383, 1017, 1591
BsmI	GAATGC	4 cut(s)	345, 1221, 1675, 2045
BsnI	GGCC	6 cut(s)	190, 258, 294, 778, 1086, 1993
Bso31I	GGTCTC	1 cut(s)	801
BsoBI	CYCGRG	1 cut(s)	1094
Bsp119I	TTCGAA	2 cut(s)	1183, 1271
Bsp1286I	GDGCHC	1 cut(s)	1742
Bsp1407I	TGTACA	1 cut(s)	1107
Bsp143I	GATC	6 cut(s)	74, 1015, 1676, 1935, 2123, 2157
Bsp1720I	GCTNAGC	1 cut(s)	1646
Bsp19I	CCATGG	2 cut(s)	452, 656
BspACI	CCGC	9 cut(s)	86, 150, 153, 375, 440, 531, 1172, 1622, 2175
BspANI	GGCC	6 cut(s)	190, 258, 294, 778, 1086, 1993
BspCNI	CTCAG	4 cut(s)	538, 562, 760, 2113
BspDI	ATCGAT	1 cut(s)	969
BspLI	GGNNCC	1 cut(s)	1678
BspMI	ACCTGC	1 cut(s)	713
BspPI	GGATC	3 cut(s)	1023, 1671, 1684
BspT104I	TTCGAA	2 cut(s)	1183, 1271
BspTI	CTTAAG	1 cut(s)	1010
BspTNI	GGTCTC	1 cut(s)	801
BsrBI	CCGCTC	1 cut(s)	440
BsrDI	GCAATG	3 cut(s)	24, 680, 715
BsrFI	RCCGGY	2 cut(s)	294, 1918
BsrGI	TGTACA	1 cut(s)	1107
BsrI	ACTGG	4 cut(s)	48, 779, 1607, 2086
BssAI	RCCGGY	2 cut(s)	294, 1918
BssECI	CCNNGG	7 cut(s)	452, 656, 1242, 1281, 1494, 1824, 1994
BssMI	GATC	6 cut(s)	74, 1015, 1676, 1935, 2123, 2157
BssSI	CACGAG	1 cut(s)	67
BssT1I	CCWWGG	3 cut(s)	452, 656, 1281
Bst2BI	CACGAG	1 cut(s)	67
Bst2UI	CCWGG	5 cut(s)	273, 423, 780, 1815, 1996
Bst4CI	ACNGT	8 cut(s)	110, 430, 694, 722, 1456, 1657, 1806, 1843
Bst6I	CTCTTC	4 cut(s)	68, 1123, 1533, 1968
BstAFI	CTTAAG	1 cut(s)	1010
BstAPI	GCANNNNNTGC	1 cut(s)	418
BstAUI	TGTACA	1 cut(s)	1107
BstBAI	YACGTR	1 cut(s)	418
BstBI	TTCGAA	2 cut(s)	1183, 1271
BstC8I	GCNNGC	4 cut(s)	671, 1064, 1206, 1624
BstDEI	CTNAG	6 cut(s)	525, 549, 768, 1555, 1646, 2100
BstDSI	CCRYGG	2 cut(s)	452, 656
BstF5I	GGATG	6 cut(s)	666, 685, 788, 835, 1159, 2090
BstHHI	GCGC	1 cut(s)	1367
BstKTI	GATC	6 cut(s)	77, 1018, 1679, 1938, 2126, 2160
BstMAI	GTCTC	3 cut(s)	552, 801, 1720
BstMBI	GATC	6 cut(s)	74, 1015, 1676, 1935, 2123, 2157
BstMWI	GCNNNNNNNGC	6 cut(s)	35, 418, 457, 629, 1619, 1924
BstNI	CCWGG	5 cut(s)	273, 423, 780, 1815, 1996
BstNSI	RCATGY	1 cut(s)	892
BstSLI	GKGCMC	1 cut(s)	1742
BstV1I	GCAGC	4 cut(s)	209, 396, 1120, 1379
BstV2I	GAAGAC	3 cut(s)	238, 424, 2130
BstX2I	RGATCY	3 cut(s)	74, 1015, 1676
BstXI	CCANNNNNNTGG	1 cut(s)	2127
BstYI	RGATCY	3 cut(s)	74, 1015, 1676
Bsu15I	ATCGAT	1 cut(s)	969
Bsu36I	CCTNAGG	1 cut(s)	525
BsuI	GTATCC	1 cut(s)	106
BsuRI	GGCC	6 cut(s)	190, 258, 294, 778, 1086, 1993
BsuTUI	ATCGAT	1 cut(s)	969
BtgI	CCRYGG	2 cut(s)	452, 656
BtsCI	GGATG	6 cut(s)	666, 685, 788, 835, 1159, 2090
BtsIMutI	CAGTG	2 cut(s)	690, 1465
BveI	ACCTGC	1 cut(s)	713
Cac8I	GCNNGC	4 cut(s)	671, 1064, 1206, 1624
CaiI	CAGNNNCTG	1 cut(s)	1873
CfoI	GCGC	1 cut(s)	1367
Cfr10I	RCCGGY	2 cut(s)	294, 1918
Cfr13I	GGNCC	5 cut(s)	362, 533, 929, 1767, 1992
ClaI	ATCGAT	1 cut(s)	969
CseI	GACGC	3 cut(s)	18, 163, 1702
Csp6I	GTAC	6 cut(s)	565, 702, 918, 1108, 1438, 1552
CviQI	GTAC	6 cut(s)	565, 702, 918, 1108, 1438, 1552
DdeI	CTNAG	6 cut(s)	525, 549, 768, 1555, 1646, 2100
DpnI	GATC	6 cut(s)	76, 1017, 1678, 1937, 2125, 2159
DpnII	GATC	6 cut(s)	74, 1015, 1676, 1935, 2123, 2157
DraI	TTTAAA	1 cut(s)	790
DriI	GACNNNNNGTC	1 cut(s)	1659
EaeI	YGGCCR	3 cut(s)	188, 256, 292
Eam1104I	CTCTTC	4 cut(s)	68, 1123, 1533, 1968
Eam1105I	GACNNNNNGTC	1 cut(s)	1659
EarI	CTCTTC	4 cut(s)	68, 1123, 1533, 1968
EciI	GGCGGA	1 cut(s)	139
Eco130I	CCWWGG	3 cut(s)	452, 656, 1281
Eco31I	GGTCTC	1 cut(s)	801
Eco47I	GGWCC	4 cut(s)	362, 533, 929, 1767
Eco57I	CTGAAG	3 cut(s)	418, 906, 1320
Eco81I	CCTNAGG	1 cut(s)	525
Eco88I	CYCGRG	1 cut(s)	1094
EcoO109I	RGGNCCY	1 cut(s)	929
EcoRII	CCWGG	5 cut(s)	271, 421, 778, 1813, 1994
EcoT14I	CCWWGG	3 cut(s)	452, 656, 1281
EcoT22I	ATGCAT	1 cut(s)	345
ErhI	CCWWGG	3 cut(s)	452, 656, 1281
Esp3I	CGTCTC	1 cut(s)	1720
FalI	AAGNNNNNCTT	2 cut(s)	498, 530
FaqI	GGGAC	4 cut(s)	161, 383, 1017, 1591
FauI	CCCGC	1 cut(s)	1615
FauNDI	CATATG	1 cut(s)	345
FbaI	TGATCA	1 cut(s)	1935
FblI	GTMKAC	1 cut(s)	32
Fnu4HI	GCNGC	8 cut(s)	87, 153, 223, 375, 410, 1134, 1368, 2176
FokI	GGATG	6 cut(s)	673, 692, 795, 842, 1166, 2097
Fsp4HI	GCNGC	8 cut(s)	87, 153, 223, 375, 410, 1134, 1368, 2176
FspBI	CTAG	4 cut(s)	987, 1664, 1799, 2004
GlaI	GCGC	1 cut(s)	1366
GluI	GCNGC	8 cut(s)	87, 153, 223, 375, 410, 1134, 1368, 2176
HaeIII	GGCC	6 cut(s)	190, 258, 294, 778, 1086, 1993
HapII	CCGG	8 cut(s)	295, 401, 1352, 1496, 1824, 1919, 2066, 2142
HgaI	GACGC	3 cut(s)	18, 163, 1702
HhaI	GCGC	1 cut(s)	1367
Hin6I	GCGC	1 cut(s)	1365
HinP1I	GCGC	1 cut(s)	1365
HincII	GTYRAC	1 cut(s)	1396
HindII	GTYRAC	1 cut(s)	1396
HindIII	AAGCTT	1 cut(s)	512
HinfI	GANTC	7 cut(s)	4, 236, 349, 396, 1115, 1511, 1579
HpaII	CCGG	8 cut(s)	295, 401, 1352, 1496, 1824, 1919, 2066, 2142
HphI	GGTGA	6 cut(s)	61, 109, 1186, 1855, 1982, 2167
Hpy166II	GTNNAC	7 cut(s)	33, 493, 877, 1108, 1110, 1396, 1440
Hpy188I	TCNGA	5 cut(s)	437, 1042, 1345, 1875, 2103
Hpy188III	TCNNGA	9 cut(s)	250, 319, 478, 859, 924, 1122, 1477, 1600, 1799
Hpy8I	GTNNAC	7 cut(s)	33, 493, 877, 1108, 1110, 1396, 1440
Hpy99I	CGWCG	2 cut(s)	484, 2027
HpyAV	CCTTC	4 cut(s)	209, 587, 1781, 1914
HpyCH4III	ACNGT	8 cut(s)	110, 430, 694, 722, 1456, 1657, 1806, 1843
HpyCH4IV	ACGT	3 cut(s)	417, 482, 916
HpyCH4V	TGCA	7 cut(s)	225, 343, 412, 521, 708, 1236, 1673
HpyF10VI	GCNNNNNNNGC	6 cut(s)	35, 418, 457, 629, 1619, 1924
HpyF3I	CTNAG	6 cut(s)	525, 549, 768, 1555, 1646, 2100
HpySE526I	ACGT	3 cut(s)	417, 482, 916
HspAI	GCGC	1 cut(s)	1365
Ksp22I	TGATCA	1 cut(s)	1935
Kzo9I	GATC	6 cut(s)	74, 1015, 1676, 1935, 2123, 2157
LmnI	GCTCC	4 cut(s)	688, 967, 1496, 1697
Lsp1109I	GCAGC	4 cut(s)	209, 396, 1120, 1379
LweI	GCATC	3 cut(s)	670, 1075, 1804
MaeI	CTAG	4 cut(s)	987, 1664, 1799, 2004
MaeII	ACGT	3 cut(s)	417, 482, 916
MaeIII	GTNAC	5 cut(s)	10, 722, 1264, 1817, 1843
MalI	GATC	6 cut(s)	76, 1017, 1678, 1937, 2125, 2159
MbiI	CCGCTC	1 cut(s)	440
MboI	GATC	6 cut(s)	74, 1015, 1676, 1935, 2123, 2157
MflI	RGATCY	3 cut(s)	74, 1015, 1676
MhlI	GDGCHC	1 cut(s)	1742
MlsI	TGGCCA	2 cut(s)	190, 258
MluCI	AATT	7 cut(s)	357, 385, 728, 907, 1072, 1747, 2057
MluNI	TGGCCA	2 cut(s)	190, 258
MlyI	GAGTC	4 cut(s)	245, 405, 1109, 1573
MmeI	TCCRAC	1 cut(s)	1675
Mox20I	TGGCCA	2 cut(s)	190, 258
Mph1103I	ATGCAT	1 cut(s)	345
MscI	TGGCCA	2 cut(s)	190, 258
MseI	TTAA	5 cut(s)	789, 1011, 1326, 1964, 2133
Msp20I	TGGCCA	2 cut(s)	190, 258
MspCI	CTTAAG	1 cut(s)	1010
MspI	CCGG	8 cut(s)	295, 401, 1352, 1496, 1824, 1919, 2066, 2142
Mva1269I	GAATGC	4 cut(s)	345, 1221, 1675, 2045
MvaI	CCWGG	5 cut(s)	273, 423, 780, 1815, 1996
MwoI	GCNNNNNNNGC	6 cut(s)	35, 418, 457, 629, 1619, 1924
NciI	CCSGG	5 cut(s)	401, 1353, 1496, 1825, 2143
NcoI	CCATGG	2 cut(s)	452, 656
NdeI	CATATG	1 cut(s)	345
NdeII	GATC	6 cut(s)	74, 1015, 1676, 1935, 2123, 2157
NlaIV	GGNNCC	1 cut(s)	1678
NmeAIII	GCCGAG	2 cut(s)	403, 1758
NmuCI	GTSAC	2 cut(s)	722, 1843
NsiI	ATGCAT	1 cut(s)	345
NspI	RCATGY	1 cut(s)	892
NspV	TTCGAA	2 cut(s)	1183, 1271
PaeR7I	CTCGAG	1 cut(s)	1094
PciI	ACATGT	1 cut(s)	888
PcsI	WCGNNNNNNNCGW	2 cut(s)	1847, 2028
PctI	GAATGC	4 cut(s)	345, 1221, 1675, 2045
PfeI	GAWTC	3 cut(s)	4, 349, 1511
PflMI	CCANNNNNTGG	3 cut(s)	272, 762, 1907
PfoI	TCCNGGA	2 cut(s)	271, 2141
PkrI	GCNGC	8 cut(s)	88, 154, 224, 376, 411, 1135, 1369, 2177
PleI	GAGTC	4 cut(s)	244, 404, 1109, 1573
PpsI	GAGTC	4 cut(s)	244, 404, 1109, 1573
Ppu21I	YACGTR	1 cut(s)	418
PpuMI	RGGWCCY	1 cut(s)	929
PscI	ACATGT	1 cut(s)	888
Psp5II	RGGWCCY	1 cut(s)	929
Psp6I	CCWGG	5 cut(s)	271, 421, 778, 1813, 1994
PspGI	CCWGG	5 cut(s)	271, 421, 778, 1813, 1994
PspN4I	GGNNCC	1 cut(s)	1678
PspPI	GGNCC	5 cut(s)	362, 533, 929, 1767, 1992
PspPPI	RGGWCCY	1 cut(s)	929
PspXI	VCTCGAGB	1 cut(s)	1094
PstNI	CAGNNNCTG	1 cut(s)	1873
PsuI	RGATCY	3 cut(s)	74, 1015, 1676
RsaI	GTAC	6 cut(s)	566, 703, 919, 1109, 1439, 1553
RsaNI	GTAC	6 cut(s)	565, 702, 918, 1108, 1438, 1552
SaqAI	TTAA	5 cut(s)	789, 1011, 1326, 1964, 2133
SatI	GCNGC	8 cut(s)	87, 153, 223, 375, 410, 1134, 1368, 2176
Sau3AI	GATC	6 cut(s)	74, 1015, 1676, 1935, 2123, 2157
Sau96I	GGNCC	5 cut(s)	362, 533, 929, 1767, 1992
ScaI	AGTACT	1 cut(s)	1553
SchI	GAGTC	4 cut(s)	245, 405, 1109, 1573
SduI	GDGCHC	1 cut(s)	1742
SfaNI	GCATC	3 cut(s)	670, 1075, 1804
Sfr274I	CTCGAG	1 cut(s)	1094
SfuI	TTCGAA	2 cut(s)	1183, 1271
SinI	GGWCC	4 cut(s)	362, 533, 929, 1767
SlaI	CTCGAG	1 cut(s)	1094
SmlI	CTYRAG	3 cut(s)	1010, 1094, 1598
SmoI	CTYRAG	3 cut(s)	1010, 1094, 1598
Sse9I	AATT	7 cut(s)	357, 385, 728, 907, 1072, 1747, 2057
SsiI	CCGC	9 cut(s)	86, 150, 153, 375, 440, 531, 1172, 1622, 2175
SspI	AATATT	2 cut(s)	646, 1636
SspMI	CTAG	4 cut(s)	987, 1664, 1799, 2004
StyI	CCWWGG	3 cut(s)	452, 656, 1281
TaaI	ACNGT	8 cut(s)	110, 430, 694, 722, 1456, 1657, 1806, 1843
TaiI	ACGT	3 cut(s)	420, 485, 919
TaqI	TCGA	7 cut(s)	81, 234, 969, 1095, 1183, 1271, 2163
TaqII	GACCGA	1 cut(s)	1256
TasI	AATT	7 cut(s)	357, 385, 728, 907, 1072, 1747, 2057
TatI	WGTACW	2 cut(s)	1107, 1551
TauI	GCSGC	4 cut(s)	89, 155, 377, 2178
TfiI	GAWTC	3 cut(s)	4, 349, 1511
Tru1I	TTAA	5 cut(s)	789, 1011, 1326, 1964, 2133
Tru9I	TTAA	5 cut(s)	789, 1011, 1326, 1964, 2133
TscAI	CASTG	2 cut(s)	697, 1465
TseFI	GTSAC	2 cut(s)	722, 1843
TseI	GCWGC	4 cut(s)	222, 409, 1133, 1367
Tsp45I	GTSAC	2 cut(s)	722, 1843
TspGWI	ACGGA	2 cut(s)	603, 912
TspRI	CASTG	2 cut(s)	697, 1465
Van91I	CCANNNNNTGG	3 cut(s)	272, 762, 1907
Vha464I	CTTAAG	1 cut(s)	1010
VpaK11BI	GGWCC	4 cut(s)	362, 533, 929, 1767
XapI	RAATTY	3 cut(s)	1072, 1747, 2057
XbaI	TCTAGA	1 cut(s)	1798
XceI	RCATGY	1 cut(s)	892
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2103
XhoI	CTCGAG	1 cut(s)	1094
XmiI	GTMKAC	1 cut(s)	32
XspI	CTAG	4 cut(s)	987, 1664, 1799, 2004
ZrmI	AGTACT	1 cut(s)	1553
Zsp2I	ATGCAT	1 cut(s)	345

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.