Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0007701

Physical Location & Seq

Genomic Coordinates Reverse (-)

9960154 .. 9970506

Gene Structure 10353 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1136400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2556 bp

ATGAAGCTGCTGCCGCCATTGCTGCTGCTTTCGCTGTTACCGTGTTGGGCAATCGCGCTATTGGCAACGGCTGCGGCGCGCAATGTGAGCTACGATAGCCGGTCTCTGATCATCGATGGCCAGCGGAAGCTCCTCATCTCTGCTGCCATTCACTACCCTCGCAGCGTCCCTGAGATGTGGCCGAAGCTAGTTCAGACGGCGAAAGAAGGAGGGGTGGATGTGATTGAGACGTATGTGTTTTGGAATGGTCATGAACCATCTCCAGGCAATTATTATTTTGGGGGACGATATGATCTGGTAAAGTTTGTCAAAATCGTCGAGCAGGCTGGGATGCATTTGATTCTTCGAATTGGCCCATTTGTTGCGGCAGAATGGTACTTTGGAGGGATACCTGTTTGGTTGCATTATGTGCCTGGCACTGTGTTTCGGACTGAAAATAAGCCTTTTAAGTATCACATGCAGAAGTTCACAACATTCATAGTTGACCTAATGAAACAGGAGAAGTTTTTTGCATCGCAAGGGGGTCCTATTATCTTGGCCCAGGTAGAAAATGAATATGGATACTATGAAAAGGATTACGGAGAAGGGGGTAAACAATATGCTATGTGGGCTGCTAGTATGGCTGTTTCTCAGAATATAGGTGTCCCTTGGATAATGTGCCAACAATTTGATGCTCCAGAGTCTGTGATAAATACTTGTAACTCCTTCTACTGTGACCAGTTTACGCCTATCTATCAGAACAAACCTAAAATTTGGACAGAGAATTGGCCTGGCTGGTTCAAAACATTTGGGGGCTGGAATCCTCACCGGCCAGCTGAAGATATTGCTTTTTCTGTAGCTCGCTTTTTCCAAAAAGGCGGAAGTGTACACAATTACTATATGTATCATGGAGGAACAAATTTTGGTCGAACTTCAGGTGGACCATTCATTACCACAAGTTACGACTATGAGGCACCAATTGATGAATATGGATTACCTAGGCTTCCAAAATGGGGGCACCTTAAGCAACTTCACAGAGCCATCAAGTTATGTGAGCATATTATGCTCAACAGTCAGCCAACTAATGTATCACTGGGTCCTTCCCTAGAGGCTGATGTCTTTACCAACTCTTCTGGAGCATGTGCTGCCTTTATAGCGAACATGGATGACAAAAATGACAAGACAGTAGAATTTCGAAACATGTCATATCATCTTCCAGCATGGTCGGTTAGCATACTGCCAGACTGCAAGAATGTAGTGTTTAATACAGCAAAGGTTGGATCCCAGAGCTCTGTAGTTGAAATGTTACCTGAGAGTTTGCAGCTATCAGTAGGATCAGCAGATAAAAGCTTGAAAGATCTGAAATGGGATGTATTTGTGGAGAAAGCTGGGATTTGGGGTGAAGCGGACTTTGTAAAAAGTGGCTTGGTAGATCACATTAACACTACAAAATTTACGACGGACTATCTTTGGTATACAACAAGCATATTGGTCGGTGAAAATGAAGAGTTCTTAAAGAAAGGAAGCTCGCCAGTTCTTCTAATTGAATCAAAGGGTCATGCTGTCCATGCTTTTGTGAATCAAGAACTCCAAGCTAGTGCAGCTGGAAATGGCACACACTTCCCCTTCAAATTAAAAACTCCCATCTCTCTGAAGGAGGGGAAGAATGATATTGCTCTACTAAGCATGACAGTTGGTCTGCAGAACGCAGGATCATTTTATGAATGGGTAGGGGCAGGGCTAACTAGTGTCAAGATTCAGGGATTCAACAATGGGACAATAGATTTGTCAGCGTATAATTGGACCTACAAGATCGGATTGGAAGGGGAGCATCAAGGTCTTGACAAGGAAGAAGGTTTCGGTAATGTAAATTGGGTTTCAGCCTCAGAACCTCCAAAGGAACAGCCTTTGACATGGTATAAGGTTATTGTGGACCCTCCATCGGGTGATGATCCTGTTGGGTTGGATATGATTCACATGGGAAAAGGTCTAGCTTGGTTAAATGGAGAAGAGATTGGAAGATACTGGCCTAGGAAGGGCCCTTTACATGGATGTGTTAAAGAATGCAATTACAGAGGCAAATTTGATCCAGACAAATGCAATACAGGGTGTGGAGAACCAACGCAAAGATGGTACCATGTTCCACGGTCTTGGTTCAAGCAATCTGGAAACGTACTGGTGATATTCGAGGAGAAAGGTGGAGATCCATCCAAAATTGAGTTCTCAAGGCGGAAAATCACTGGTGTGTGCGCTCTTGTAGCAGAGAATTACCCCTCTATTGACCTTGAATCTTGGAATGAGGGAAGTGGAAGTAACGAAACCATTGCAACTGTCCATTTAGGTTGCCCTGAGGATACACATATTTCAAGTGTAAAATTTGCCAGCTTTGGGAATCCTACAGGCGCCTGTGGGTCCTATACACAGGGCAACTGCCATGACCCTAACTCCATTTCTGTGGTCGAAAAGGTCTGCTTAAATAAGAGCCAATGCGACATAGAATTAACAGAAGAAAATTTTAATAAGGGTTCGTGTCTGAGTGAACCAAAGAAGCTTGCGGTTGAAGTAGAGTGCAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0008150 GO:0009505 GO:0009628 GO:0015925 GO:0016787 GO:0016798 GO:0030312 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0071944 GO:0080167

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Gal_Lectin Glyco_hydro_35

Protein Analysis

852

Amino Acids

94.82

Weight (kDa)

5.89

Isoelectric Point (pI)

39.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	35 - 340	9.3e-116	Glycosyl hydrolases family 35
GHD	PF17834	363 - 419	5.3e-25	Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd	PF21467	630 - 717	4.5e-21	Beta-galactosidase, galactose-binding domain
SUEL_Lectin	PF02140	773 - 850	1.5e-16	D-galactoside/L-rhamnose binding SUEL lectin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007701)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63810
fragaria_vesca	FvH4_1g25130 FvH4_1g25130 FvH4_1g25130 FvH4_1g25130 FvH4_1g25130
malus_domestica	MD15G1136400.v1.1
prunus_persica	Prupe.4G278500_v2.0.a1 Prupe.4G278500_v2.0.a1 Prupe.4G278500_v2.0.a1
pyrus_communis	pycom15g12300
rosa_chinensis	RchiOBHm_Chr2g0122791
rosa_laevigata	RLG00000018715
rosa_multiflora	Rmu_sc0008528.1_g000013 Rmu_sc0037008.1_g000001
rosa_roxburghii	Rroxscaffold_2G00121170
rosa_rugosa	Rorug02G0242600 Rorug02G0242700 Rorug02G0242700
rosa_samantha	Rh2AG301300 Rh2BG308700 Rh2CG287400 Rh2DG325500
rosa_wichuraiana	Rw2G023890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	2112
AccB1I	GGYRCC	4 cut(s)	952, 996, 2112, 2381
AccI	GTMKAC	1 cut(s)	1454
AccII	CGCG	2 cut(s)	56, 79
AciI	CCGC	8 cut(s)	14, 74, 124, 365, 858, 1385, 2209, 2534
AclWI	GGATC	7 cut(s)	1254, 1267, 1321, 1699, 1925, 2060, 2177
AcoI	YGGCCR	3 cut(s)	118, 179, 809
AcsI	RAATTY	7 cut(s)	750, 898, 1169, 1430, 2060, 2354, 2491
AcuI	CTGAAG	3 cut(s)	837, 897, 1652
AcyI	GRCGYC	1 cut(s)	2382
AfaI	GTAC	4 cut(s)	377, 867, 2114, 2154
AfiI	CCNNNNNNNGG	7 cut(s)	263, 992, 1921, 1922, 2015, 2027, 2367
AflII	CTTAAG	1 cut(s)	1001
AflIII	ACRYGT	1 cut(s)	1179
AhdI	GACNNNNNGTC	1 cut(s)	1674
AhlI	ACTAGT	1 cut(s)	1724
AjnI	CCWGG	4 cut(s)	262, 412, 540, 769
AjuI	GAANNNNNNNTTGG	4 cut(s)	363, 395, 2183, 2215
AleI	CACNNNNGTG	1 cut(s)	2222
Alw21I	GWGCWC	1 cut(s)	1271
Alw26I	GTCTC	2 cut(s)	108, 221
AlwI	GGATC	7 cut(s)	1254, 1267, 1321, 1699, 1925, 2060, 2177
AlwNI	CAGNNNCTG	1 cut(s)	683
AoxI	GGCC	8 cut(s)	118, 179, 352, 537, 767, 809, 2006, 2017
ApaI	GGGCCC	1 cut(s)	2021
ApoI	RAATTY	7 cut(s)	750, 898, 1169, 1430, 2060, 2354, 2491
ArsI	GACNNNNNNTTYG	2 cut(s)	1747, 1779
Asp718I	GGTACC	1 cut(s)	2112
AspA2I	CCTAGG	2 cut(s)	977, 2009
AspLEI	GCGC	5 cut(s)	58, 79, 81, 2231, 2384
AsuHPI	GGTGA	5 cut(s)	797, 1391, 1487, 1937, 2170
AsuII	TTCGAA	2 cut(s)	346, 1174
AvaII	GGWCC	6 cut(s)	524, 920, 1076, 1782, 1912, 2391
AvrII	CCTAGG	2 cut(s)	977, 2009
AxyI	CCTNAGG	1 cut(s)	2328
BaeGI	GKGCMC	2 cut(s)	999, 2021
BaeI	ACNNNNGTAYC	2 cut(s)	1050, 1083
BalI	TGGCCA	1 cut(s)	120
BamHI	GGATCC	1 cut(s)	1259
BanI	GGYRCC	4 cut(s)	952, 996, 2112, 2381
BanII	GRGCYC	2 cut(s)	1271, 2021
Bbv12I	GWGCWC	1 cut(s)	1271
BbvI	GCAGC	9 cut(s)	9, 12, 58, 130, 174, 598, 1111, 1312, 1592
BccI	CCATC	7 cut(s)	110, 265, 1028, 1631, 1927, 2103, 2194
BceAI	ACGGC	2 cut(s)	84, 213
BcgI	CGANNNNNNTGC	4 cut(s)	1453, 1487, 2285, 2319
BciT130I	CCWGG	4 cut(s)	264, 414, 542, 771
BciVI	GTATCC	3 cut(s)	381, 554, 2326
BclI	TGATCA	1 cut(s)	108
BcoDI	GTCTC	2 cut(s)	108, 221
BcuI	ACTAGT	1 cut(s)	1724
BfaI	CTAG	8 cut(s)	188, 615, 978, 1085, 1575, 1725, 1970, 2010
BfmI	CTRYAG	4 cut(s)	834, 1272, 1679, 2376
BfoI	RGCGCY	1 cut(s)	2385
BfrI	CTTAAG	1 cut(s)	1001
BfuI	GTATCC	3 cut(s)	381, 554, 2326
BglII	AGATCT	1 cut(s)	1336
BlnI	CCTAGG	2 cut(s)	977, 2009
Bme1390I	CCNGG	4 cut(s)	264, 414, 542, 771
Bme18I	GGWCC	6 cut(s)	524, 920, 1076, 1782, 1912, 2391
BmeRI	GACNNNNNGTC	1 cut(s)	1674
BmrFI	CCNGG	4 cut(s)	264, 414, 542, 771
BmrI	ACTGGG	1 cut(s)	1082
BmsI	GCATC	4 cut(s)	321, 521, 661, 1819
BmuI	ACTGGG	1 cut(s)	1082
BpmI	CTGGAG	3 cut(s)	246, 660, 1134
Bpu14I	TTCGAA	2 cut(s)	346, 1174
BpuEI	CTTGAG	1 cut(s)	2188
Bsa29I	ATCGAT	1 cut(s)	114
BsaHI	GRCGYC	1 cut(s)	2382
BsaI	GGTCTC	1 cut(s)	108
BsaJI	CCNNGG	5 cut(s)	540, 647, 977, 2009, 2123
BsaXI	ACNNNNNCTCC	2 cut(s)	2408, 2438
Bsc4I	CCNNNNNNNGG	7 cut(s)	263, 992, 1921, 1922, 2015, 2027, 2367
Bse118I	RCCGGY	2 cut(s)	99, 807
Bse1I	ACTGG	6 cut(s)	718, 1077, 1511, 2009, 2160, 2224
Bse21I	CCTNAGG	1 cut(s)	2328
Bse3DI	GCAATG	3 cut(s)	17, 88, 2301
BseBI	CCWGG	4 cut(s)	264, 414, 542, 771
BseCI	ATCGAT	1 cut(s)	114
BseDI	CCNNGG	5 cut(s)	540, 647, 977, 2009, 2123
BseGI	GGATG	6 cut(s)	223, 336, 1150, 1354, 2036, 2186
BseLI	CCNNNNNNNGG	7 cut(s)	263, 992, 1921, 1922, 2015, 2027, 2367
BseMI	GCAATG	3 cut(s)	17, 88, 2301
BseMII	CTCAG	6 cut(s)	162, 644, 1281, 1878, 2319, 2504
BseNI	ACTGG	6 cut(s)	718, 1077, 1511, 2009, 2160, 2224
BsePI	GCGCGC	1 cut(s)	77
BseRI	GAGGAG	2 cut(s)	122, 2183
BseSI	GKGCMC	2 cut(s)	999, 2021
BseXI	GCAGC	9 cut(s)	9, 12, 58, 130, 174, 598, 1111, 1312, 1592
BseYI	CCCAGC	2 cut(s)	326, 1367
BsgI	GTGCAG	1 cut(s)	1599
Bsh1236I	CGCG	2 cut(s)	56, 79
BshFI	GGCC	8 cut(s)	120, 181, 354, 539, 769, 811, 2008, 2019
BshNI	GGYRCC	4 cut(s)	952, 996, 2112, 2381
BshVI	ATCGAT	1 cut(s)	114
BsiHKAI	GWGCWC	1 cut(s)	1271
BsiSI	CCGG	2 cut(s)	100, 808
BslFI	GGGAC	4 cut(s)	152, 297, 629, 1768
BslI	CCNNNNNNNGG	7 cut(s)	263, 992, 1921, 1922, 2015, 2027, 2367
BsmAI	GTCTC	2 cut(s)	108, 221
BsmBI	CGTCTC	1 cut(s)	221
BsmFI	GGGAC	4 cut(s)	152, 297, 629, 1768
BsmI	GAATGC	1 cut(s)	2048
BsnI	GGCC	8 cut(s)	120, 181, 354, 539, 769, 811, 2008, 2019
Bso31I	GGTCTC	1 cut(s)	108
Bsp119I	TTCGAA	2 cut(s)	346, 1174
Bsp120I	GGGCCC	1 cut(s)	2017
Bsp1286I	GDGCHC	3 cut(s)	999, 1271, 2021
Bsp1407I	TGTACA	1 cut(s)	865
BspACI	CCGC	8 cut(s)	14, 74, 124, 365, 858, 1385, 2209, 2534
BspANI	GGCC	8 cut(s)	120, 181, 354, 539, 769, 811, 2008, 2019
BspCNI	CTCAG	6 cut(s)	163, 643, 1282, 1877, 2320, 2505
BspDI	ATCGAT	1 cut(s)	114
BspFNI	CGCG	2 cut(s)	56, 79
BspHI	TCATGA	1 cut(s)	250
BspMAI	CTGCAG	1 cut(s)	1683
BspPI	GGATC	7 cut(s)	1254, 1267, 1321, 1699, 1925, 2060, 2177
BspT104I	TTCGAA	2 cut(s)	346, 1174
BspT107I	GGYRCC	4 cut(s)	952, 996, 2112, 2381
BspTI	CTTAAG	1 cut(s)	1001
BspTNI	GGTCTC	1 cut(s)	108
BsrDI	GCAATG	3 cut(s)	17, 88, 2301
BsrFI	RCCGGY	2 cut(s)	99, 807
BsrGI	TGTACA	1 cut(s)	865
BsrI	ACTGG	6 cut(s)	718, 1077, 1511, 2009, 2160, 2224
BssAI	RCCGGY	2 cut(s)	99, 807
BssECI	CCNNGG	5 cut(s)	540, 647, 977, 2009, 2123
BssHII	GCGCGC	1 cut(s)	77
BssNAI	GTATAC	1 cut(s)	1455
BssNI	GRCGYC	1 cut(s)	2382
BssT1I	CCWWGG	3 cut(s)	647, 977, 2009
Bst1107I	GTATAC	1 cut(s)	1455
Bst2UI	CCWGG	4 cut(s)	264, 414, 542, 771
Bst4CI	ACNGT	8 cut(s)	42, 421, 713, 1052, 1165, 1672, 2127, 2311
Bst6I	CTCTTC	3 cut(s)	1114, 1479, 1983
BstACI	GRCGYC	1 cut(s)	2382
BstAFI	CTTAAG	1 cut(s)	1001
BstAPI	GCANNNNNTGC	4 cut(s)	71, 409, 1042, 1124
BstAUI	TGTACA	1 cut(s)	865
BstBI	TTCGAA	2 cut(s)	346, 1174
BstC8I	GCNNGC	8 cut(s)	79, 122, 324, 813, 841, 1508, 2362, 2532
BstDEI	CTNAG	7 cut(s)	171, 630, 1290, 1661, 1864, 2328, 2513
BstDSI	CCRYGG	1 cut(s)	2123
BstF5I	GGATG	6 cut(s)	223, 336, 1150, 1354, 2036, 2186
BstFNI	CGCG	2 cut(s)	56, 79
BstH2I	RGCGCY	1 cut(s)	2385
BstHHI	GCGC	5 cut(s)	58, 79, 81, 2231, 2384
BstMAI	GTCTC	2 cut(s)	108, 221
BstNI	CCWGG	4 cut(s)	264, 414, 542, 771
BstNSI	RCATGY	3 cut(s)	460, 1122, 1183
BstSCI	CCNGG	4 cut(s)	262, 412, 540, 769
BstSFI	CTRYAG	4 cut(s)	834, 1272, 1679, 2376
BstSLI	GKGCMC	2 cut(s)	999, 2021
BstUI	CGCG	2 cut(s)	56, 79
BstV1I	GCAGC	9 cut(s)	9, 12, 58, 130, 174, 598, 1111, 1312, 1592
BstX2I	RGATCY	3 cut(s)	1259, 1336, 2182
BstXI	CCANNNNNNTGG	2 cut(s)	2130, 2434
BstYI	RGATCY	3 cut(s)	1259, 1336, 2182
BstZ17I	GTATAC	1 cut(s)	1455
Bsu15I	ATCGAT	1 cut(s)	114
Bsu36I	CCTNAGG	1 cut(s)	2328
BsuI	GTATCC	3 cut(s)	381, 554, 2326
BsuRI	GGCC	8 cut(s)	120, 181, 354, 539, 769, 811, 2008, 2019
BsuTUI	ATCGAT	1 cut(s)	114
BtgI	CCRYGG	1 cut(s)	2123
BtgZI	GCGATG	1 cut(s)	498
BtsCI	GGATG	6 cut(s)	223, 336, 1150, 1354, 2036, 2186
BtsIMutI	CAGTG	3 cut(s)	417, 1070, 2217
Cac8I	GCNNGC	8 cut(s)	79, 122, 324, 813, 841, 1508, 2362, 2532
CaiI	CAGNNNCTG	1 cut(s)	683
CciI	TCATGA	1 cut(s)	250
CfoI	GCGC	5 cut(s)	58, 79, 81, 2231, 2384
Cfr10I	RCCGGY	2 cut(s)	99, 807
ClaI	ATCGAT	1 cut(s)	114
CseI	GACGC	1 cut(s)	154
Csp6I	GTAC	4 cut(s)	376, 866, 2113, 2153
CviQI	GTAC	4 cut(s)	376, 866, 2113, 2153
DdeI	CTNAG	7 cut(s)	171, 630, 1290, 1661, 1864, 2328, 2513
DinI	GGCGCC	1 cut(s)	2383
DriI	GACNNNNNGTC	1 cut(s)	1674
EaeI	YGGCCR	3 cut(s)	118, 179, 809
Eam1104I	CTCTTC	3 cut(s)	1114, 1479, 1983
Eam1105I	GACNNNNNGTC	1 cut(s)	1674
EarI	CTCTTC	3 cut(s)	1114, 1479, 1983
EciI	GGCGGA	2 cut(s)	873, 2224
Ecl136II	GAGCTC	1 cut(s)	1269
Eco130I	CCWWGG	3 cut(s)	647, 977, 2009
Eco24I	GRGCYC	2 cut(s)	1271, 2021
Eco31I	GGTCTC	1 cut(s)	108
Eco47I	GGWCC	6 cut(s)	524, 920, 1076, 1782, 1912, 2391
Eco53kI	GAGCTC	1 cut(s)	1269
Eco57I	CTGAAG	3 cut(s)	837, 897, 1652
Eco81I	CCTNAGG	1 cut(s)	2328
EcoICRI	GAGCTC	1 cut(s)	1269
EcoO109I	RGGNCCY	5 cut(s)	524, 1076, 2017, 2018, 2391
EcoRII	CCWGG	4 cut(s)	262, 412, 540, 769
EcoT14I	CCWWGG	3 cut(s)	647, 977, 2009
EcoT22I	ATGCAT	1 cut(s)	336
EcoT38I	GRGCYC	2 cut(s)	1271, 2021
EgeI	GGCGCC	1 cut(s)	2383
EheI	GGCGCC	1 cut(s)	2383
ErhI	CCWWGG	3 cut(s)	647, 977, 2009
Esp3I	CGTCTC	1 cut(s)	221
FalI	AAGNNNNNCTT	2 cut(s)	2435, 2467
FaqI	GGGAC	4 cut(s)	152, 297, 629, 1768
FbaI	TGATCA	1 cut(s)	108
FblI	GTMKAC	1 cut(s)	1454
FokI	GGATG	6 cut(s)	230, 343, 1157, 1361, 2043, 2173
FriOI	GRGCYC	2 cut(s)	1271, 2021
FspBI	CTAG	8 cut(s)	188, 615, 978, 1085, 1575, 1725, 1970, 2010
GlaI	GCGC	5 cut(s)	57, 78, 80, 2230, 2383
GsaI	CCCAGC	2 cut(s)	330, 1371
GsuI	CTGGAG	3 cut(s)	246, 660, 1134
HaeII	RGCGCY	1 cut(s)	2385
HaeIII	GGCC	8 cut(s)	120, 181, 354, 539, 769, 811, 2008, 2019
HapII	CCGG	2 cut(s)	100, 808
HgaI	GACGC	1 cut(s)	154
HhaI	GCGC	5 cut(s)	58, 79, 81, 2231, 2384
Hin1I	GRCGYC	1 cut(s)	2382
Hin6I	GCGC	5 cut(s)	56, 77, 79, 2229, 2382
HinP1I	GCGC	5 cut(s)	56, 77, 79, 2229, 2382
HincII	GTYRAC	1 cut(s)	484
HindII	GTYRAC	1 cut(s)	484
HindIII	AAGCTT	2 cut(s)	1327, 2528
HpaII	CCGG	2 cut(s)	100, 808
HphI	GGTGA	5 cut(s)	797, 1391, 1487, 1937, 2170
Hpy188III	TCNNGA	8 cut(s)	251, 677, 1113, 1562, 1732, 1820, 2069, 2145
Hpy99I	CGWCG	2 cut(s)	320, 1441
HpyAV	CCTTC	9 cut(s)	200, 578, 715, 1089, 1615, 1627, 1796, 1826, 2008
HpyCH4III	ACNGT	8 cut(s)	42, 421, 713, 1052, 1165, 1672, 2127, 2311
HpyCH4IV	ACGT	2 cut(s)	230, 2151
HpyF3I	CTNAG	7 cut(s)	171, 630, 1290, 1661, 1864, 2328, 2513
HpySE526I	ACGT	2 cut(s)	230, 2151
Hsp92I	GRCGYC	1 cut(s)	2382
HspAI	GCGC	5 cut(s)	56, 77, 79, 2229, 2382
KasI	GGCGCC	1 cut(s)	2381
KpnI	GGTACC	1 cut(s)	2116
Ksp22I	TGATCA	1 cut(s)	108
LmnI	GCTCC	4 cut(s)	135, 679, 1115, 1807
Lsp1109I	GCAGC	9 cut(s)	9, 12, 58, 130, 174, 598, 1111, 1312, 1592
LweI	GCATC	4 cut(s)	321, 521, 661, 1819
MaeI	CTAG	8 cut(s)	188, 615, 978, 1085, 1575, 1725, 1970, 2010
MaeII	ACGT	2 cut(s)	230, 2151
MaeIII	GTNAC	6 cut(s)	36, 698, 713, 938, 1284, 2291
MfeI	CAATTG	1 cut(s)	957
MflI	RGATCY	3 cut(s)	1259, 1336, 2182
MhlI	GDGCHC	3 cut(s)	999, 1271, 2021
MlsI	TGGCCA	1 cut(s)	120
MluNI	TGGCCA	1 cut(s)	120
Mly113I	GGCGCC	1 cut(s)	2382
MlyI	GAGTC	1 cut(s)	689
MmeI	TCCRAC	2 cut(s)	1237, 1923
Mox20I	TGGCCA	1 cut(s)	120
Mph1103I	ATGCAT	1 cut(s)	336
MscI	TGGCCA	1 cut(s)	120
MslI	CAYNNNNRTG	3 cut(s)	2031, 2222, 2432
Msp20I	TGGCCA	1 cut(s)	120
MspA1I	CMGCKG	3 cut(s)	124, 815, 1583
MspCI	CTTAAG	1 cut(s)	1001
MspI	CCGG	2 cut(s)	100, 808
MspR9I	CCNGG	4 cut(s)	264, 414, 542, 771
MunI	CAATTG	1 cut(s)	957
Mva1269I	GAATGC	1 cut(s)	2048
MvaI	CCWGG	4 cut(s)	264, 414, 542, 771
MvnI	CGCG	2 cut(s)	56, 79
NarI	GGCGCC	1 cut(s)	2382
NmuCI	GTSAC	1 cut(s)	713
NsiI	ATGCAT	1 cut(s)	336
NspI	RCATGY	3 cut(s)	460, 1122, 1183
NspV	TTCGAA	2 cut(s)	346, 1174
OliI	CACNNNNGTG	1 cut(s)	2222
PagI	TCATGA	1 cut(s)	250
PauI	GCGCGC	1 cut(s)	77
PciI	ACATGT	1 cut(s)	1179
PcsI	WCGNNNNNNNCGW	1 cut(s)	38
PctI	GAATGC	1 cut(s)	2048
PfeI	GAWTC	9 cut(s)	340, 799, 1526, 1558, 1735, 1743, 1951, 2267, 2371
PleI	GAGTC	1 cut(s)	688
PluTI	GGCGCC	1 cut(s)	2385
PpsI	GAGTC	1 cut(s)	688
PpuMI	RGGWCCY	3 cut(s)	524, 1076, 2391
PscI	ACATGT	1 cut(s)	1179
Psp124BI	GAGCTC	1 cut(s)	1271
Psp5II	RGGWCCY	3 cut(s)	524, 1076, 2391
Psp6I	CCWGG	4 cut(s)	262, 412, 540, 769
PspFI	CCCAGC	2 cut(s)	326, 1367
PspGI	CCWGG	4 cut(s)	262, 412, 540, 769
PspOMI	GGGCCC	1 cut(s)	2017
PspPPI	RGGWCCY	3 cut(s)	524, 1076, 2391
PstI	CTGCAG	1 cut(s)	1683
PstNI	CAGNNNCTG	1 cut(s)	683
PsuI	RGATCY	3 cut(s)	1259, 1336, 2182
PteI	GCGCGC	1 cut(s)	77
PvuII	CAGCTG	2 cut(s)	815, 1583
RsaI	GTAC	4 cut(s)	377, 867, 2114, 2154
RsaNI	GTAC	4 cut(s)	376, 866, 2113, 2153
RseI	CAYNNNNRTG	3 cut(s)	2031, 2222, 2432
SacI	GAGCTC	1 cut(s)	1271
SchI	GAGTC	1 cut(s)	689
ScrFI	CCNGG	4 cut(s)	264, 414, 542, 771
SduI	GDGCHC	3 cut(s)	999, 1271, 2021
SfaNI	GCATC	4 cut(s)	321, 521, 661, 1819
SfcI	CTRYAG	4 cut(s)	834, 1272, 1679, 2376
SfoI	GGCGCC	1 cut(s)	2383
SfuI	TTCGAA	2 cut(s)	346, 1174
SinI	GGWCC	6 cut(s)	524, 920, 1076, 1782, 1912, 2391
SmiMI	CAYNNNNRTG	3 cut(s)	2031, 2222, 2432
SmlI	CTYRAG	2 cut(s)	1001, 2203
SmoI	CTYRAG	2 cut(s)	1001, 2203
SpeI	ACTAGT	1 cut(s)	1724
SsiI	CCGC	8 cut(s)	14, 74, 124, 365, 858, 1385, 2209, 2534
SspDI	GGCGCC	1 cut(s)	2381
SspMI	CTAG	8 cut(s)	188, 615, 978, 1085, 1575, 1725, 1970, 2010
SstI	GAGCTC	1 cut(s)	1271
StyD4I	CCNGG	4 cut(s)	262, 412, 540, 769
StyI	CCWWGG	3 cut(s)	647, 977, 2009
TaaI	ACNGT	8 cut(s)	42, 421, 713, 1052, 1165, 1672, 2127, 2311
TaiI	ACGT	2 cut(s)	233, 2154
TaqI	TCGA	7 cut(s)	114, 318, 346, 907, 1174, 2166, 2439
TatI	WGTACW	1 cut(s)	865
TauI	GCSGC	3 cut(s)	16, 77, 368
TfiI	GAWTC	9 cut(s)	340, 799, 1526, 1558, 1735, 1743, 1951, 2267, 2371
TscAI	CASTG	3 cut(s)	424, 1077, 2224
TseFI	GTSAC	1 cut(s)	713
Tsp45I	GTSAC	1 cut(s)	713
TspGWI	ACGGA	2 cut(s)	594, 1454
TspRI	CASTG	3 cut(s)	424, 1077, 2224
Vha464I	CTTAAG	1 cut(s)	1001
VpaK11BI	GGWCC	6 cut(s)	524, 920, 1076, 1782, 1912, 2391
XapI	RAATTY	7 cut(s)	750, 898, 1169, 1430, 2060, 2354, 2491
XceI	RCATGY	3 cut(s)	460, 1122, 1183
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2106
XmaJI	CCTAGG	2 cut(s)	977, 2009
XmiI	GTMKAC	1 cut(s)	1454
XspI	CTAG	8 cut(s)	188, 615, 978, 1085, 1575, 1725, 1970, 2010
Zsp2I	ATGCAT	1 cut(s)	336

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.