RchiOBHm_Chr2g0122791

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0007701
Physical Location & Seq
Reverse (-)
35837440 .. 35847346
9907 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49516

Sequence Viewer

Length: 2508 bp
ATGAAGATACTAGCTTGGCTGCTTTCTTTGCTACTGTGCTTCACATTGTGTTTGGGATACAATGTGAGCTACGACAGCCGCTCTTTGATAATCAATGGCCAAAGAAAGCTGCTCATTTCTGCTGCCATTCACTACCCTCGTAGTGTCCCTGCGATGTGGCCGGGGCTAGTTCAGACGGCGAAAGAAGGAGGGGTTGATGTGATTGAGACATATGTGTTTTGGAATGGTCATGAACCGTCTCCAGGCAATTATTATTTTGGAGGACGATATGATCTGGTCAAATTTGCGAAGATCGTGGAGGATGCTGGAATGTATTTGATTCTTCGAATTGGCCCATTCGTTGCAGCAGAATGGTACTTTGGCGGGGTACCTGTGTGGTTGCATTATGTGCCTGACACTGTATTTCGAACTGACAATGAGCCCTTTAAGTATCACATGCAGAAGTTCACAACGTTCATAGTTAATCTAATGAAACAGGAGAAGCTTTTTGCACCTCAAGGAGGTCCAATTATTTTGGCCCAGATAGAAAATGAATATGGATACTACGAAAAAGATTATGGAGAGGGGGGCAAAAAATATGCACAGTGGTCTGCTAGCATGGCTGTTGCTCAGAATATAGGTGTGCCATGGATCATGTGCCAACAGTTTGATGCTCCAGAGTCTGTGATAAATACATGTAACTCCTTCTACTGTGACCAATTTACTCCTATTTTTCCGAACAAACCTAAAATTTGGACAGAGAATTGGCCTGGCTGGTTTAAAACATTTGGGGCCAGAAATCCTCACAGGCCAGCTGAAGATATTGCTTTTTCTGTAGCTCGTTTTTTCCAAAAAGGTGGCAGTGTACAAAATTACTACATGTATCATGGAGGAACAAATTTTGGTCGCACTTCAGGTGGACCATTTATAACCACAAGTTATGACTATGAGGCACCAATCGATGAATATGGATTACCAAGGCTTCCAAAATGGGCACATCTTAAGCAACTTCACCTAGCAATTAAGTTATGTGAGCATGCTTTGCTCAACAGTGAACCCACTCATGTATCGTTGGGTCCTTCGGAAGAGGCTGATGTTTTCACCAACTCTTCTGGAGCATGTGCTGCCTTTATTTCTAATACGGACGACAAAAATGACAAAACAGTAGTATTTCGTAATGTGTCATATCATCTACCAGCATGGTCAGTTAGCATACTGCCTGACTGCAAGACTGTAGTATTCAACACAGCAAAGGTAGCATCCCAAACCTCTGTAGTTGAGATGGCATCTGAAAATAAAGGCTTGAAAGGTCTTAAATGGGAGGTATTTGTGGAGAAAGCTGGGATCTGGGGTGAAGCTGACTTTGTTAAAACTGGATTTGTAGATCACATCAATACCACAAAATTTACCACTGACTATCTGTGGTATACAACAAGCATATTTGTTGATGAAAATGAAGCGTTCTTAAAGAAAGGAAGCTCACCAGTTCTTCTAATTGAATCAAAAGGCCATGCTCTCCATGCTTTTGTCAATGATGAACTTCAAGATAGTGCATCTGGAAATGGCACGCATCCACCCTTCACTTTAAAGAGTCCTATCTCTCTGAAGGCAGGGAAGAATGAAATTGCTCTACTCAGTATGACAGTTGGTCTGCAGAATGCGGGATCATTTTATGAGTGGGTAGGGGCAGGATTAACTAGTGTCAAGATTAAGGGTTTCAACAATGGTACAATTGATTTGTCTGCACATAATTGGACCTACAAGATTGGGGTGCAAGGAGAACACCTAGGACTTTACAAGAAAGATGGCTCAAGTAATGTAACTTGGGTTTCAACCCCAGACCCGCCAAAGAAACAACCTTTGACGTGGTATAAGGCTATTGTGGACCCTCCGCCAGGTGATGAACCTATTGGACTGGATATGATTGACATGGGAAAAGGTCTGGCTTGGTTGAATGGAGAAGAAATTGGAAGATACTGGCCTAGGAAGAGCCCTACACATGGATGTGTTCAAGAATGCAATTATAGAGGAAAATTTGATCCAGACAAATGCAATACAGGATGTGGAGAACCAACACAGAGATGGTACCATGTTCCACGGTCTTGGTTCAAACCATCTGGAAACGTTCTGGTGATATTTGAGGAGAAAGGTGGAGATCCATTGAAAATTGAGTTTTCGAGAAGGAAAATCTCAGGTGCCTGTGCTCATGCTGCAGTAGATTACCCCTCTGTTGATCTTGAATCTTGGCGTGAGGAAAATGGAACTAACAAAAGCATTTCAACCATTAACTTGAAGTGCCCTGAGAACACACGTATATCTACTGTAAAATTTGCCAGCTTTGGAAATCCTACGGGCTCCTGTGGATCCTACACCAAAGGTGACTGCCACGATCCTAACTCCACTTCTGTGGTTGAAAAGATATGCTTAAACCAAAACAAATGTGCCATAGAATTGGCAGAAGAGAAATTTAGTAGGGGTTTGTGTCCGAGTGTAACGAAGAAGCTTGCAGTTGAAGTAGTGTGCGGCTGA

Protein Analysis

835

Amino Acids

92.99

Weight (kDa)

6.61

Isoelectric Point (pI)

35.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 28 - 332 3.4e-116 Glycosyl hydrolases family 35
GHD PF17834 356 - 412 8.2e-25 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 614 - 701 1.1e-20 Beta-galactosidase, galactose-binding domain
SUEL_Lectin PF02140 757 - 834 8.5e-18 D-galactoside/L-rhamnose binding SUEL lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 908
Acc65I GGTACC 2 cut(s) 367, 2064
AccB1I GGYRCC 4 cut(s) 367, 931, 2064, 2174
AccBSI CCGCTC 1 cut(s) 81
AccI GTMKAC 1 cut(s) 1406
AciI CCGC 6 cut(s) 79, 363, 1640, 1823, 1871, 2502
AclI AACGTT 2 cut(s) 452, 2103
AclWI GGATC 8 cut(s) 638, 1331, 1651, 2012, 2129, 2337, 2350, 2363
AcoI YGGCCR 2 cut(s) 97, 158
AcsI RAATTY 7 cut(s) 281, 729, 877, 1382, 2012, 2306, 2444
AcuI CTGAAG 3 cut(s) 816, 876, 1604
AdeI CACNNNGTG 1 cut(s) 48
AfaI GTAC 5 cut(s) 356, 369, 846, 1708, 2066
AfiI CCNNNNNNNGG 4 cut(s) 242, 500, 1874, 1979
AflII CTTAAG 1 cut(s) 980
AflIII ACRYGT 3 cut(s) 674, 858, 2288
AhdI GACNNNNNGTC 1 cut(s) 1626
AhlI ACTAGT 1 cut(s) 1676
AjiI CACGTC 1 cut(s) 1845
AjnI CCWGG 3 cut(s) 241, 748, 1873
AjuI GAANNNNNNNTTGG 2 cut(s) 342, 374
AleI CACNNNNGTG 1 cut(s) 2384
Alw21I GWGCWC 1 cut(s) 2185
Alw26I GTCTC 2 cut(s) 200, 243
AlwI GGATC 8 cut(s) 638, 1331, 1651, 2012, 2129, 2337, 2350, 2363
AlwNI CAGNNNCTG 1 cut(s) 662
AoxI GGCC 9 cut(s) 97, 158, 331, 516, 746, 771, 788, 1486, 1958
ApeKI GCWGC 6 cut(s) 19, 109, 122, 344, 1103, 2189
ApoI RAATTY 7 cut(s) 281, 729, 877, 1382, 2012, 2306, 2444
Asp718I GGTACC 2 cut(s) 367, 2064
AspA2I CCTAGG 2 cut(s) 1765, 1961
AspS9I GGNCC 8 cut(s) 332, 503, 517, 771, 899, 1055, 1734, 1864
AsuC2I CCSGG 1 cut(s) 162
AsuHPI GGTGA 7 cut(s) 983, 1072, 1343, 1452, 1889, 2122, 2369
AsuII TTCGAA 2 cut(s) 325, 406
AsuNHI GCTAGC 1 cut(s) 593
AvaII GGWCC 5 cut(s) 503, 899, 1055, 1734, 1864
AvrII CCTAGG 2 cut(s) 1765, 1961
BaeGI GKGCMC 2 cut(s) 976, 2279
BaeI ACNNNNGTAYC 4 cut(s) 359, 392, 1029, 1062
BalI TGGCCA 1 cut(s) 99
BamHI GGATCC 1 cut(s) 2342
BanI GGYRCC 4 cut(s) 367, 931, 2064, 2174
BanII GRGCYC 3 cut(s) 423, 1973, 2336
Bbv12I GWGCWC 1 cut(s) 2185
BbvI GCAGC 6 cut(s) 6, 96, 109, 356, 1090, 2176
BccI CCATC 4 cut(s) 1255, 1778, 2055, 2101
BceAI ACGGC 1 cut(s) 192
BciT130I CCWGG 3 cut(s) 243, 750, 1875
BciVI GTATCC 2 cut(s) 50, 533
BcnI CCSGG 1 cut(s) 162
BcoDI GTCTC 2 cut(s) 200, 243
BcuI ACTAGT 1 cut(s) 1676
BfaI CTAG 7 cut(s) 11, 167, 594, 995, 1677, 1766, 1962
BfmI CTRYAG 5 cut(s) 813, 1212, 1251, 1631, 2190
BfrI CTTAAG 1 cut(s) 980
BfuI GTATCC 2 cut(s) 50, 533
BisI GCNGC 8 cut(s) 20, 79, 110, 123, 345, 1104, 2190, 2503
BlnI CCTAGG 2 cut(s) 1765, 1961
BlsI GCNGC 8 cut(s) 21, 80, 111, 124, 346, 1105, 2191, 2504
Bme1390I CCNGG 4 cut(s) 162, 243, 750, 1875
Bme18I GGWCC 5 cut(s) 503, 899, 1055, 1734, 1864
BmeRI GACNNNNNGTC 1 cut(s) 1626
BmgBI CACGTC 1 cut(s) 1845
BmgT120I GGNCC 8 cut(s) 332, 503, 517, 771, 899, 1055, 1734, 1864
BmiI GGNNCC 9 cut(s) 369, 772, 933, 1056, 1866, 2066, 2176, 2335, 2344
BmrFI CCNGG 4 cut(s) 162, 243, 750, 1875
BmsI GCATC 6 cut(s) 292, 640, 1247, 1274, 1541, 1558
BmtI GCTAGC 1 cut(s) 597
BpmI CTGGAG 3 cut(s) 225, 639, 1113
Bpu14I TTCGAA 2 cut(s) 325, 406
BpuEI CTTGAG 2 cut(s) 480, 1774
BpuMI CCSGG 1 cut(s) 162
Bsa29I ATCGAT 1 cut(s) 939
BsaAI YACGTR 1 cut(s) 2291
BsaJI CCNNGG 6 cut(s) 161, 626, 956, 1765, 1961, 2075
Bsc4I CCNNNNNNNGG 4 cut(s) 242, 500, 1874, 1979
Bse1I ACTGG 4 cut(s) 1357, 1463, 1899, 1961
BseBI CCWGG 3 cut(s) 243, 750, 1875
BseCI ATCGAT 1 cut(s) 939
BseDI CCNNGG 6 cut(s) 161, 626, 956, 1765, 1961, 2075
BseGI GGATG 5 cut(s) 307, 1238, 1549, 1988, 2045
BseLI CCNNNNNNNGG 4 cut(s) 242, 500, 1874, 1979
BseMII CTCAG 4 cut(s) 623, 1627, 2184, 2271
BseNI ACTGG 4 cut(s) 1357, 1463, 1899, 1961
BseRI GAGGAG 1 cut(s) 2135
BseSI GKGCMC 2 cut(s) 976, 2279
BseXI GCAGC 6 cut(s) 6, 96, 109, 356, 1090, 2176
BseYI CCCAGC 1 cut(s) 1319
BsgI GTGCAG 1 cut(s) 1707
BshFI GGCC 9 cut(s) 99, 160, 333, 518, 748, 773, 790, 1488, 1960
BshNI GGYRCC 4 cut(s) 367, 931, 2064, 2174
BshVI ATCGAT 1 cut(s) 939
BsiHKAI GWGCWC 1 cut(s) 2185
BsiSI CCGG 1 cut(s) 161
BslFI GGGAC 1 cut(s) 131
BslI CCNNNNNNNGG 4 cut(s) 242, 500, 1874, 1979
BsmAI GTCTC 2 cut(s) 200, 243
BsmBI CGTCTC 1 cut(s) 243
BsmFI GGGAC 1 cut(s) 131
BsmI GAATGC 2 cut(s) 1642, 2000
BsnI GGCC 9 cut(s) 99, 160, 333, 518, 748, 773, 790, 1488, 1960
Bsp119I TTCGAA 2 cut(s) 325, 406
Bsp1286I GDGCHC 6 cut(s) 423, 976, 1973, 2185, 2279, 2336
Bsp1407I TGTACA 1 cut(s) 844
Bsp19I CCATGG 1 cut(s) 626
BspACI CCGC 6 cut(s) 79, 363, 1640, 1823, 1871, 2502
BspANI GGCC 9 cut(s) 99, 160, 333, 518, 748, 773, 790, 1488, 1960
BspCNI CTCAG 4 cut(s) 622, 1626, 2183, 2272
BspDI ATCGAT 1 cut(s) 939
BspHI TCATGA 1 cut(s) 229
BspLI GGNNCC 9 cut(s) 369, 772, 933, 1056, 1866, 2066, 2176, 2335, 2344
BspMAI CTGCAG 2 cut(s) 1635, 2194
BspOI GCTAGC 1 cut(s) 597
BspPI GGATC 8 cut(s) 638, 1331, 1651, 2012, 2129, 2337, 2350, 2363
BspQI GCTCTTC 1 cut(s) 1961
BspT104I TTCGAA 2 cut(s) 325, 406
BspT107I GGYRCC 4 cut(s) 367, 931, 2064, 2174
BspTI CTTAAG 1 cut(s) 980
BsrBI CCGCTC 1 cut(s) 81
BsrGI TGTACA 1 cut(s) 844
BsrI ACTGG 4 cut(s) 1357, 1463, 1899, 1961
BssECI CCNNGG 6 cut(s) 161, 626, 956, 1765, 1961, 2075
BssNAI GTATAC 1 cut(s) 1407
BssT1I CCWWGG 4 cut(s) 626, 956, 1765, 1961
Bst1107I GTATAC 1 cut(s) 1407
Bst2UI CCWGG 3 cut(s) 243, 750, 1875
Bst6I CTCTTC 4 cut(s) 1059, 1093, 1961, 2433
BstAFI CTTAAG 1 cut(s) 980
BstAPI GCANNNNNTGC 3 cut(s) 388, 1021, 1103
BstAUI TGTACA 1 cut(s) 844
BstBAI YACGTR 1 cut(s) 2291
BstBI TTCGAA 2 cut(s) 325, 406
BstC8I GCNNGC 6 cut(s) 595, 792, 1017, 1547, 2314, 2484
BstDEI CTNAG 4 cut(s) 609, 1613, 2170, 2280
BstDSI CCRYGG 2 cut(s) 626, 2075
BstENI CCTNNNNNAGG 2 cut(s) 498, 1872
BstF5I GGATG 5 cut(s) 307, 1238, 1549, 1988, 2045
BstMAI GTCTC 2 cut(s) 200, 243
BstMWI GCNNNNNNNGC 9 cut(s) 28, 75, 388, 599, 1021, 1103, 1235, 1499, 2189
BstNI CCWGG 3 cut(s) 243, 750, 1875
BstNSI RCATGY 5 cut(s) 439, 678, 862, 1019, 1101
BstSCI CCNGG 4 cut(s) 160, 241, 748, 1873
BstSFI CTRYAG 5 cut(s) 813, 1212, 1251, 1631, 2190
BstSLI GKGCMC 2 cut(s) 976, 2279
BstV1I GCAGC 6 cut(s) 6, 96, 109, 356, 1090, 2176
BstX2I RGATCY 3 cut(s) 1323, 2134, 2342
BstXI CCANNNNNNTGG 4 cut(s) 836, 2082, 2386, 2431
BstYI RGATCY 3 cut(s) 1323, 2134, 2342
BstZ17I GTATAC 1 cut(s) 1407
Bsu15I ATCGAT 1 cut(s) 939
BsuI GTATCC 2 cut(s) 50, 533
BsuRI GGCC 9 cut(s) 99, 160, 333, 518, 748, 773, 790, 1488, 1960
BsuTUI ATCGAT 1 cut(s) 939
BtgI CCRYGG 2 cut(s) 626, 2075
BtgZI GCGATG 1 cut(s) 167
BtrI CACGTC 1 cut(s) 1845
BtsCI GGATG 5 cut(s) 307, 1238, 1549, 1988, 2045
BtsI GCAGTG 1 cut(s) 847
BtsIMutI CAGTG 5 cut(s) 396, 590, 847, 1036, 1389
Cac8I GCNNGC 6 cut(s) 595, 792, 1017, 1547, 2314, 2484
CaiI CAGNNNCTG 1 cut(s) 662
CciI TCATGA 1 cut(s) 229
Cfr13I GGNCC 8 cut(s) 332, 503, 517, 771, 899, 1055, 1734, 1864
ClaI ATCGAT 1 cut(s) 939
Csp6I GTAC 5 cut(s) 355, 368, 845, 1707, 2065
CviQI GTAC 5 cut(s) 355, 368, 845, 1707, 2065
DdeI CTNAG 4 cut(s) 609, 1613, 2170, 2280
DraI TTTAAA 2 cut(s) 760, 1566
DraIII CACNNNGTG 1 cut(s) 48
DriI GACNNNNNGTC 1 cut(s) 1626
EaeI YGGCCR 2 cut(s) 97, 158
Eam1104I CTCTTC 4 cut(s) 1059, 1093, 1961, 2433
Eam1105I GACNNNNNGTC 1 cut(s) 1626
EarI CTCTTC 4 cut(s) 1059, 1093, 1961, 2433
EciI GGCGGA 1 cut(s) 1860
Eco130I CCWWGG 4 cut(s) 626, 956, 1765, 1961
Eco24I GRGCYC 3 cut(s) 423, 1973, 2336
Eco47I GGWCC 5 cut(s) 503, 899, 1055, 1734, 1864
Eco57I CTGAAG 3 cut(s) 816, 876, 1604
EcoNI CCTNNNNNAGG 2 cut(s) 498, 1872
EcoO109I RGGNCCY 1 cut(s) 1055
EcoRII CCWGG 3 cut(s) 241, 748, 1873
EcoT14I CCWWGG 4 cut(s) 626, 956, 1765, 1961
EcoT38I GRGCYC 3 cut(s) 423, 1973, 2336
ErhI CCWWGG 4 cut(s) 626, 956, 1765, 1961
Esp3I CGTCTC 1 cut(s) 243
FalI AAGNNNNNCTT 2 cut(s) 2387, 2419
FaqI GGGAC 1 cut(s) 131
FauI CCCGC 3 cut(s) 356, 1633, 1830
FauNDI CATATG 1 cut(s) 211
FblI GTMKAC 1 cut(s) 1406
Fnu4HI GCNGC 8 cut(s) 20, 79, 110, 123, 345, 1104, 2190, 2503
FokI GGATG 5 cut(s) 314, 1225, 1536, 1995, 2052
FriOI GRGCYC 3 cut(s) 423, 1973, 2336
Fsp4HI GCNGC 8 cut(s) 20, 79, 110, 123, 345, 1104, 2190, 2503
FspBI CTAG 7 cut(s) 11, 167, 594, 995, 1677, 1766, 1962
GluI GCNGC 8 cut(s) 20, 79, 110, 123, 345, 1104, 2190, 2503
GsaI CCCAGC 1 cut(s) 1323
GsuI CTGGAG 3 cut(s) 225, 639, 1113
HaeIII GGCC 9 cut(s) 99, 160, 333, 518, 748, 773, 790, 1488, 1960
HapII CCGG 1 cut(s) 161
HindIII AAGCTT 2 cut(s) 482, 2480
HinfI GANTC 5 cut(s) 319, 659, 1478, 1570, 2219
HpaII CCGG 1 cut(s) 161
HphI GGTGA 7 cut(s) 983, 1072, 1343, 1452, 1889, 2122, 2369
Hpy166II GTNNAC 6 cut(s) 447, 845, 899, 1034, 1407, 1864
Hpy188I TCNGA 7 cut(s) 174, 612, 717, 1063, 1270, 1584, 2466
Hpy8I GTNNAC 6 cut(s) 447, 845, 899, 1034, 1407, 1864
HpyAV CCTTC 6 cut(s) 179, 694, 1068, 1567, 1579, 2154
HpyCH4IV ACGT 4 cut(s) 452, 1844, 2103, 2290
HpyF10VI GCNNNNNNNGC 9 cut(s) 28, 75, 388, 599, 1021, 1103, 1235, 1499, 2189
HpyF3I CTNAG 4 cut(s) 609, 1613, 2170, 2280
HpySE526I ACGT 4 cut(s) 452, 1844, 2103, 2290
KpnI GGTACC 2 cut(s) 371, 2068
LguI GCTCTTC 1 cut(s) 1961
LmnI GCTCC 3 cut(s) 658, 1094, 2339
Lsp1109I GCAGC 6 cut(s) 6, 96, 109, 356, 1090, 2176
LweI GCATC 6 cut(s) 292, 640, 1247, 1274, 1541, 1558
MaeI CTAG 7 cut(s) 11, 167, 594, 995, 1677, 1766, 1962
MaeII ACGT 4 cut(s) 452, 1844, 2103, 2290
MaeIII GTNAC 5 cut(s) 677, 692, 1798, 2357, 2470
MbiI CCGCTC 1 cut(s) 81
MfeI CAATTG 1 cut(s) 1710
MflI RGATCY 3 cut(s) 1323, 2134, 2342
MhlI GDGCHC 6 cut(s) 423, 976, 1973, 2185, 2279, 2336
MlsI TGGCCA 1 cut(s) 99
MluNI TGGCCA 1 cut(s) 99
MlyI GAGTC 2 cut(s) 668, 1579
Mox20I TGGCCA 1 cut(s) 99
MscI TGGCCA 1 cut(s) 99
MslI CAYNNNNRTG 4 cut(s) 1981, 1983, 2059, 2384
Msp20I TGGCCA 1 cut(s) 99
MspA1I CMGCKG 1 cut(s) 794
MspCI CTTAAG 1 cut(s) 980
MspI CCGG 1 cut(s) 161
MspR9I CCNGG 4 cut(s) 162, 243, 750, 1875
MunI CAATTG 1 cut(s) 1710
Mva1269I GAATGC 2 cut(s) 1642, 2000
MvaI CCWGG 3 cut(s) 243, 750, 1875
MwoI GCNNNNNNNGC 9 cut(s) 28, 75, 388, 599, 1021, 1103, 1235, 1499, 2189
NciI CCSGG 1 cut(s) 162
NcoI CCATGG 1 cut(s) 626
NdeI CATATG 1 cut(s) 211
NheI GCTAGC 1 cut(s) 593
NlaIV GGNNCC 9 cut(s) 369, 772, 933, 1056, 1866, 2066, 2176, 2335, 2344
NmuCI GTSAC 2 cut(s) 692, 2357
NspI RCATGY 5 cut(s) 439, 678, 862, 1019, 1101
NspV TTCGAA 2 cut(s) 325, 406
OliI CACNNNNGTG 1 cut(s) 2384
PaeI GCATGC 1 cut(s) 1019
PagI TCATGA 1 cut(s) 229
PciI ACATGT 2 cut(s) 674, 858
PciSI GCTCTTC 1 cut(s) 1961
PctI GAATGC 2 cut(s) 1642, 2000
PfeI GAWTC 3 cut(s) 319, 1478, 2219
PkrI GCNGC 8 cut(s) 21, 80, 111, 124, 346, 1105, 2191, 2504
PleI GAGTC 2 cut(s) 667, 1578
PpsI GAGTC 2 cut(s) 667, 1578
Ppu21I YACGTR 1 cut(s) 2291
PpuMI RGGWCCY 1 cut(s) 1055
PscI ACATGT 2 cut(s) 674, 858
PsiI TTATAA 1 cut(s) 908
Psp1406I AACGTT 2 cut(s) 452, 2103
Psp5II RGGWCCY 1 cut(s) 1055
Psp6I CCWGG 3 cut(s) 241, 748, 1873
PspFI CCCAGC 1 cut(s) 1319
PspGI CCWGG 3 cut(s) 241, 748, 1873
PspN4I GGNNCC 9 cut(s) 369, 772, 933, 1056, 1866, 2066, 2176, 2335, 2344
PspPI GGNCC 8 cut(s) 332, 503, 517, 771, 899, 1055, 1734, 1864
PspPPI RGGWCCY 1 cut(s) 1055
PstI CTGCAG 2 cut(s) 1635, 2194
PstNI CAGNNNCTG 1 cut(s) 662
PsuI RGATCY 3 cut(s) 1323, 2134, 2342
PvuII CAGCTG 1 cut(s) 794
RsaI GTAC 5 cut(s) 356, 369, 846, 1708, 2066
RsaNI GTAC 5 cut(s) 355, 368, 845, 1707, 2065
RseI CAYNNNNRTG 4 cut(s) 1981, 1983, 2059, 2384
SapI GCTCTTC 1 cut(s) 1961
SatI GCNGC 8 cut(s) 20, 79, 110, 123, 345, 1104, 2190, 2503
Sau96I GGNCC 8 cut(s) 332, 503, 517, 771, 899, 1055, 1734, 1864
SchI GAGTC 2 cut(s) 668, 1579
ScrFI CCNGG 4 cut(s) 162, 243, 750, 1875
SduI GDGCHC 6 cut(s) 423, 976, 1973, 2185, 2279, 2336
SfaNI GCATC 6 cut(s) 292, 640, 1247, 1274, 1541, 1558
SfcI CTRYAG 5 cut(s) 813, 1212, 1251, 1631, 2190
SfuI TTCGAA 2 cut(s) 325, 406
SinI GGWCC 5 cut(s) 503, 899, 1055, 1734, 1864
SmiMI CAYNNNNRTG 4 cut(s) 1981, 1983, 2059, 2384
SmlI CTYRAG 3 cut(s) 495, 980, 1789
SmoI CTYRAG 3 cut(s) 495, 980, 1789
SpeI ACTAGT 1 cut(s) 1676
SphI GCATGC 1 cut(s) 1019
SsiI CCGC 6 cut(s) 79, 363, 1640, 1823, 1871, 2502
SspMI CTAG 7 cut(s) 11, 167, 594, 995, 1677, 1766, 1962
StyD4I CCNGG 4 cut(s) 160, 241, 748, 1873
StyI CCWWGG 4 cut(s) 626, 956, 1765, 1961
TaiI ACGT 4 cut(s) 455, 1847, 2106, 2293
TaqI TCGA 4 cut(s) 325, 406, 939, 2156
TatI WGTACW 1 cut(s) 844
TauI GCSGC 2 cut(s) 81, 2505
TfiI GAWTC 3 cut(s) 319, 1478, 2219
TscAI CASTG 5 cut(s) 403, 590, 847, 1036, 1396
TseFI GTSAC 2 cut(s) 692, 2357
TseI GCWGC 6 cut(s) 19, 109, 122, 344, 1103, 2189
Tsp45I GTSAC 2 cut(s) 692, 2357
TspGWI ACGGA 1 cut(s) 1136
TspRI CASTG 5 cut(s) 403, 590, 847, 1036, 1396
Vha464I CTTAAG 1 cut(s) 980
VpaK11BI GGWCC 5 cut(s) 503, 899, 1055, 1734, 1864
XagI CCTNNNNNAGG 2 cut(s) 498, 1872
XapI RAATTY 7 cut(s) 281, 729, 877, 1382, 2012, 2306, 2444
XceI RCATGY 5 cut(s) 439, 678, 862, 1019, 1101
XcmI CCANNNNNNNNNTGG 1 cut(s) 2058
XmaJI CCTAGG 2 cut(s) 1765, 1961
XmiI GTMKAC 1 cut(s) 1406
XspI CTAG 7 cut(s) 11, 167, 594, 995, 1677, 1766, 1962
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.