AT5G63930
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011826
Physical Location & Seq
Forward (+)
25581696 .. 25586630
4935 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G63930.1

Sequence Viewer

Length: 3309 bp
ATGGTAAAGGAAATGATGAAGCTTGCTGTTTTTTTCATATCTCTTCTTCTTATCCTTCTGATCTCTGAGACAACAGGGTTGAACTTAGAAGGTCAATATCTTCTTGAAATCAAGAGCAAGTTTGTGGATGCTAAGCAGAATTTGAGAAACTGGAATTCAAATGATTCTGTACCTTGTGGATGGACTGGCGTGATGTGCAGCAACTATTCGAGTGATCCAGAGGTTTTGTCCTTGAATCTGTCTTCGATGGTTCTCTCGGGTAAGCTATCACCAAGCATAGGTGGATTGGTTCATCTGAAGCAGCTGGATCTGTCATATAATGGGTTGTCAGGGAAAATTCCTAAGGAAATTGGCAACTGTTCAAGCTTGGAGATTCTGAAACTAAACAATAACCAGTTTGATGGTGAGATACCTGTGGAAATAGGTAAGCTTGTGTCTTTGGAGAATCTGATCATATACAACAACAGAATCTCAGGGTCTCTCCCTGTGGAGATTGGGAACCTTTTGTCTCTCTCTCAACTGGTGACTTACAGTAACAACATCAGTGGACAATTGCCCCGTTCCATCGGCAACCTCAAGAGACTGACAAGCTTCCGAGCCGGTCAGAACATGATCTCTGGAAGTTTGCCTTCTGAGATTGGTGGATGTGAGAGCTTGGTCATGCTTGGTCTTGCACAGAACCAGTTGAGTGGTGAATTACCAAAAGAGATTGGAATGCTCAAGAAACTTTCACAGGTGATTCTTTGGGAAAATGAGTTCTCAGGGTTTATCCCCAGGGAGATTAGCAACTGCACAAGTTTAGAAACCCTTGCTCTCTACAAGAACCAGCTTGTCGGGCCAATCCCGAAAGAGCTCGGGGACCTTCAGTCGCTGGAGTTTCTCTACCTTTACAGAAACGGGTTGAATGGAACGATTCCAAGAGAGATTGGGAATCTCTCATATGCAATTGAGATAGATTTCTCGGAAAACGCTTTGACCGGAGAGATACCTTTGGAGTTAGGGAACATAGAGGGGCTTGAGCTTCTTTATCTTTTTGAGAACCAACTCACAGGTACCATCCCTGTGGAGCTTTCTACTTTGAAGAATCTATCAAAGCTTGATCTCTCCATCAATGCTTTAACGGGTCCTATTCCCCTGGGATTTCAGTATCTAAGAGGACTCTTCATGCTGCAGCTGTTTCAGAACTCCCTTAGCGGGACCATACCTCCAAAACTTGGCTGGTATAGCGATCTCTGGGTTCTTGATATGTCCGATAACCATCTCAGTGGAAGAATTCCAAGCTACCTCTGCCTTCACTCCAATATGATCATCTTGAACTTGGGTACAAACAACCTCTCTGGCAACATTCCTACTGGAATCACCACTTGCAAAACTCTGGTCCAACTTCGTCTAGCCAGAAACAACCTCGTTGGTCGGTTCCCATCCAACCTGTGTAAACAGGTTAATGTCACAGCCATCGAGTTGGGACAGAACAGATTCCGTGGTTCCATCCCTAGGGAGGTCGGGAACTGCAGTGCCCTACAAAGGCTGCAGCTAGCAGACAATGGCTTCACGGGGGAGCTACCTAGAGAGATTGGCATGCTTTCACAGCTTGGGACCTTGAATATCTCATCCAATAAGCTTACTGGAGAGGTCCCTTCTGAAATCTTCAACTGCAAGATGCTTCAGCGACTAGACATGTGTTGCAACAATTTCTCTGGAACCTTACCAAGTGAGGTAGGTTCCCTCTACCAGCTTGAGCTTCTGAAGCTCTCAAACAATAATCTTTCGGGTACAATACCTGTGGCTCTAGGGAACTTATCTCGCTTGACCGAACTACAAATGGGTGGAAACTTGTTCAACGGTAGCATTCCACGGGAGTTGGGCAGTCTGACGGGTTTGCAAATAGCATTGAACCTCAGTTATAACAAGCTTACCGGAGAAATACCACCTGAGCTCAGTAATCTTGTTATGCTTGAGTTCCTGCTCCTCAACAACAACAATTTGTCTGGGGAAATACCAAGCTCTTTTGCTAATCTCTCCAGCTTGCTTGGTTACAACTTCTCATACAACAGCTTAACTGGTCCTATTCCACTTCTCCGTAACATATCCATGTCTAGCTTCATCGGTAATGAAGGGCTTTGCGGCCCGCCGCTCAACCAGTGTATCCAAACACAGCCTTTCGCGCCTTCTCAGTCAACCGGGAAGCCCGGAGGAATGCGTTCGAGTAAAATCATAGCCATTACTGCAGCTGTGATTGGCGGGGTCTCTCTCATGCTGATTGCTCTCATTGTCTACCTCATGAGACGCCCAGTGAGAACAGTCGCTTCTTCTGCTCAAGATGGCCAACCATCGGAAATGTCTTTGGACATATACTTCCCTCCAAAGGAAGGATTCACGTTCCAAGATTTAGTTGCAGCCACTGATAATTTCGACGAGAGCTTTGTAGTTGGGAGAGGAGCTTGTGGAACCGTCTACAAAGCGGTTCTTCCAGCCGGGTACACTCTAGCTGTCAAGAAGCTGGCATCAAACCATGAAGGCGGCAACAACAACAATGTGGATAATAGCTTTAGAGCAGAGATTCTAACTCTTGGGAATATCAGGCACCGTAACATCGTCAAGCTACATGGATTCTGCAATCACCAAGGATCAAATCTGCTTCTGTATGAGTACATGCCCAAGGGAAGCCTTGGAGAAATACTTCATGATCCTTCATGCAACTTGGATTGGTCTAAGAGATTTAAAATCGCTTTAGGTGCGGCTCAGGGTCTCGCCTATTTGCACCATGATTGCAAGCCGAGAATCTTCCACCGGGACATAAAATCAAACAATATACTGCTTGACGACAAGTTTGAAGCTCATGTGGGAGATTTTGGACTAGCAAAAGTGATTGACATGCCACATTCTAAATCCATGTCTGCTATTGCTGGCTCCTATGGCTACATTGCCCCAGAATATGCATACACCATGAAAGTGACAGAGAAGTCTGACATCTATAGCTACGGGGTGGTTCTACTGGAGCTGCTAACCGGAAAAGCCCCGGTTCAGCCAATAGATCAAGGAGGAGATGTGGTGAATTGGGTGAGGAGTTACATAAGAAGAGATGCATTGTCCTCAGGAGTTCTTGATGCTCGGTTAACGCTAGAAGACGAGAGAATTGTCTCTCATATGCTCACTGTGTTAAAGATTGCACTTCTTTGCACAAGTGTGTCTCCAGTCGCGAGGCCATCGATGCGACAAGTTGTTCTCATGCTTATTGAGTCTGAGAGATCGGAAGGAGAACAAGAACATTTGGATACAGAAGAATTGACACAAACAACTACCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

1102

Amino Acids

120.48

Weight (kDa)

5.76

Isoelectric Point (pI)

36.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 28 - 67 2.5e-11 Leucine rich repeat N-terminal domain
LRR_14 PF23598 91 - 198 4.4e-13 Leucine-rich repeat region
LRR_14 PF23598 497 - 560 7.5e-06 Leucine-rich repeat region
LRR_14 PF23598 543 - 628 4.6e-08 Leucine-rich repeat region
LRR_8 PF13855 555 - 612 3.9e-07 Leucine rich repeat
Pkinase PF00069 809 - 1074 3.6e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 809 - 1022 2.3e-41 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011826)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G63930 AT5G63930
fragaria_vesca FvH4_6g01320 FvH4_6g01320
malus_domestica MD04G1235000.v1.1 MD12G1253000.v1.1
prunus_persica Prupe.6G354900_v2.0.a1 Prupe.6G354900_v2.0.a1
pyrus_communis pycom04g20870 pycom12g23210
rosa_chinensis RchiOBHm_Chr3g0448891
rosa_laevigata RLG00000025869
rosa_multiflora Rmu_co8340729.1_g000001 Rmu_sc0003116.1_g000020
rosa_rugosa Rorug02G0617000
rosa_samantha Rh3AG015200 Rh3CG013900 Rh3DG015500
rosa_wichuraiana Rw3G001130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1905
AasI GACNNNNNNGTC 1 cut(s) 2962
Acc65I GGTACC 1 cut(s) 1052
AccB1I GGYRCC 2 cut(s) 1052, 2583
AccB7I CCANNNNNTGG 1 cut(s) 1214
AccBSI CCGCTC 1 cut(s) 2134
AccI GTMKAC 2 cut(s) 2274, 2454
AccII CGCG 2 cut(s) 2165, 3200
AciI CCGC 8 cut(s) 1194, 2124, 2129, 2132, 2241, 2462, 2520, 2738
AclWI GGATC 4 cut(s) 209, 315, 2635, 2681
AcoI YGGCCR 1 cut(s) 2323
AcsI RAATTY 4 cut(s) 139, 154, 336, 1272
AcuI CTGAAG 4 cut(s) 317, 848, 1649, 1766
AcyI GRCGYC 1 cut(s) 2287
AfaI GTAC 6 cut(s) 171, 1054, 1324, 1774, 2480, 2651
AfiI CCNNNNNNNGG 9 cut(s) 278, 1194, 1214, 1494, 1498, 1524, 2332, 2365, 2369
AflIII ACRYGT 1 cut(s) 1677
AhdI GACNNNNNGTC 1 cut(s) 865
AjnI CCWGG 2 cut(s) 773, 1134
AleI CACNNNNGTG 1 cut(s) 3185
AloI GAACNNNNNNTCC 2 cut(s) 740, 772
Alw21I GWGCWC 2 cut(s) 855, 1938
Alw26I GTCTC 9 cut(s) 62, 483, 513, 574, 2251, 2278, 2753, 3145, 3195
AlwI GGATC 4 cut(s) 209, 315, 2635, 2681
AlwNI CAGNNNCTG 2 cut(s) 871, 2402
Ama87I CYCGRG 2 cut(s) 256, 854
AoxI GGCC 4 cut(s) 836, 2125, 2323, 3203
ApeKI GCWGC 9 cut(s) 198, 301, 1168, 1171, 1528, 1531, 2228, 2396, 3001
ApoI RAATTY 4 cut(s) 139, 154, 336, 1272
ArsI GACNNNNNNTTYG 6 cut(s) 1362, 1394, 2405, 2437, 2813, 2845
Asp700I GAANNNNTTC 3 cut(s) 1475, 2200, 2679
Asp718I GGTACC 1 cut(s) 1052
AspA2I CCTAGG 1 cut(s) 1493
AspLEI GCGC 1 cut(s) 2167
AspS9I GGNCC 9 cut(s) 836, 859, 1124, 1197, 1378, 1596, 1633, 2063, 2126
AsuC2I CCSGG 5 cut(s) 2182, 2190, 2476, 2792, 3020
AsuHPI GGTGA 9 cut(s) 261, 416, 535, 704, 748, 1351, 2612, 3064, 3073
AsuNHI GCTAGC 1 cut(s) 1534
AvaI CYCGRG 2 cut(s) 256, 854
AvaII GGWCC 7 cut(s) 859, 1124, 1197, 1378, 1596, 1633, 2063
AvrII CCTAGG 1 cut(s) 1493
AxyI CCTNAGG 2 cut(s) 342, 3094
BaeGI GKGCMC 1 cut(s) 1519
BaeI ACNNNNGTAYC 2 cut(s) 2128, 2161
BalI TGGCCA 1 cut(s) 2325
BanI GGYRCC 2 cut(s) 1052, 2583
BanII GRGCYC 2 cut(s) 855, 1938
BbsI GAAGAC 2 cut(s) 234, 3132
Bbv12I GWGCWC 2 cut(s) 855, 1938
BbvI GCAGC 9 cut(s) 210, 313, 1155, 1183, 1515, 1543, 2240, 2408, 2988
BciT130I CCWGG 2 cut(s) 775, 1136
BciVI GTATCC 2 cut(s) 2156, 3268
BclI TGATCA 2 cut(s) 450, 1305
BcnI CCSGG 5 cut(s) 2182, 2190, 2476, 2792, 3020
BcoDI GTCTC 9 cut(s) 62, 483, 513, 574, 2251, 2278, 2753, 3145, 3195
BfmI CTRYAG 5 cut(s) 1169, 1510, 1529, 2226, 2974
BfuI GTATCC 2 cut(s) 2156, 3268
BlnI CCTAGG 1 cut(s) 1493
BlpI GCTNAGC 1 cut(s) 132
Bme1390I CCNGG 7 cut(s) 775, 1136, 2182, 2190, 2476, 2792, 3020
Bme18I GGWCC 7 cut(s) 859, 1124, 1197, 1378, 1596, 1633, 2063
BmeRI GACNNNNNGTC 1 cut(s) 865
BmeT110I CYCGRG 2 cut(s) 256, 854
BmgT120I GGNCC 9 cut(s) 836, 859, 1124, 1197, 1378, 1596, 1633, 2063, 2126
BmrFI CCNGG 7 cut(s) 775, 1136, 2182, 2190, 2476, 2792, 3020
BmrI ACTGGG 1 cut(s) 2285
BmsI GCATC 6 cut(s) 118, 1650, 2513, 3073, 3097, 3201
BmtI GCTAGC 1 cut(s) 1538
BmuI ACTGGG 1 cut(s) 2285
BpiI GAAGAC 2 cut(s) 234, 3132
BpmI CTGGAG 5 cut(s) 893, 1647, 2005, 3017, 3177
Bpu10I CCTNAGC 3 cut(s) 1190, 1932, 2742
Bpu1102I GCTNAGC 1 cut(s) 132
BpuEI CTTGAG 6 cut(s) 560, 704, 1037, 1757, 1976, 2301
BpuMI CCSGG 5 cut(s) 2182, 2190, 2476, 2792, 3020
Bsa29I ATCGAT 1 cut(s) 3209
BsaBI GATNNNNATC 1 cut(s) 1257
BsaHI GRCGYC 1 cut(s) 2287
BsaI GGTCTC 3 cut(s) 483, 2251, 2753
BsaWI WCCGGW 3 cut(s) 977, 1916, 3008
BsaXI ACNNNNNCTCC 6 cut(s) 1189, 1219, 3033, 3036, 3063, 3066
Bsc4I CCNNNNNNNGG 9 cut(s) 278, 1194, 1214, 1494, 1498, 1524, 2332, 2365, 2369
Bse118I RCCGGY 1 cut(s) 599
Bse21I CCTNAGG 2 cut(s) 342, 3094
Bse3DI GCAATG 1 cut(s) 2922
Bse8I GATNNNNATC 1 cut(s) 1257
BseBI CCWGG 2 cut(s) 775, 1136
BseCI ATCGAT 1 cut(s) 3209
BseGI GGATG 7 cut(s) 133, 185, 650, 1056, 1421, 1488, 1610
BseJI GATNNNNATC 1 cut(s) 1257
BseLI CCNNNNNNNGG 9 cut(s) 278, 1194, 1214, 1494, 1498, 1524, 2332, 2365, 2369
BseMI GCAATG 1 cut(s) 2922
BseRI GAGGAG 4 cut(s) 1958, 2451, 3057, 3079
BseSI GKGCMC 1 cut(s) 1519
BseXI GCAGC 9 cut(s) 210, 313, 1155, 1183, 1515, 1543, 2240, 2408, 2988
BsgI GTGCAG 2 cut(s) 217, 775
Bsh1236I CGCG 2 cut(s) 2165, 3200
BshFI GGCC 4 cut(s) 838, 2127, 2325, 3205
BshNI GGYRCC 2 cut(s) 1052, 2583
BshVI ATCGAT 1 cut(s) 3209
BsiHKAI GWGCWC 2 cut(s) 855, 1938
BsiHKCI CYCGRG 2 cut(s) 256, 854
BsiSI CCGG 9 cut(s) 600, 978, 1917, 2181, 2190, 2475, 2791, 3009, 3020
BslFI GGGAC 6 cut(s) 872, 1210, 1479, 1609, 1619, 2807
BslI CCNNNNNNNGG 9 cut(s) 278, 1194, 1214, 1494, 1498, 1524, 2332, 2365, 2369
BsmAI GTCTC 9 cut(s) 62, 483, 513, 574, 2251, 2278, 2753, 3145, 3195
BsmBI CGTCTC 1 cut(s) 2278
BsmFI GGGAC 6 cut(s) 872, 1210, 1479, 1609, 1619, 2807
BsmI GAATGC 3 cut(s) 720, 1848, 2202
BsnI GGCC 4 cut(s) 838, 2127, 2325, 3205
Bso31I GGTCTC 3 cut(s) 483, 2251, 2753
BsoBI CYCGRG 2 cut(s) 256, 854
Bsp1286I GDGCHC 3 cut(s) 855, 1519, 1938
Bsp1720I GCTNAGC 1 cut(s) 132
Bsp68I TCGCGA 1 cut(s) 3200
BspACI CCGC 8 cut(s) 1194, 2124, 2129, 2132, 2241, 2462, 2520, 2738
BspANI GGCC 4 cut(s) 838, 2127, 2325, 3205
BspDI ATCGAT 1 cut(s) 3209
BspFNI CGCG 2 cut(s) 2165, 3200
BspHI TCATGA 2 cut(s) 2280, 2683
BspMAI CTGCAG 4 cut(s) 1173, 1514, 1533, 2230
BspOI GCTAGC 1 cut(s) 1538
BspPI GGATC 4 cut(s) 209, 315, 2635, 2681
BspT107I GGYRCC 2 cut(s) 1052, 2583
BspTNI GGTCTC 3 cut(s) 483, 2251, 2753
BsrBI CCGCTC 1 cut(s) 2134
BsrDI GCAATG 1 cut(s) 2922
BsrFI RCCGGY 1 cut(s) 599
BssAI RCCGGY 1 cut(s) 599
BssNI GRCGYC 1 cut(s) 2287
BssT1I CCWWGG 4 cut(s) 1493, 2623, 2658, 2668
Bst2UI CCWGG 2 cut(s) 775, 1136
Bst4CI ACNGT 7 cut(s) 359, 533, 1844, 2302, 2452, 2588, 3157
Bst6I CTCTTC 3 cut(s) 48, 1166, 3073
BstACI GRCGYC 1 cut(s) 2287
BstC8I GCNNGC 8 cut(s) 24, 1536, 1580, 2027, 2129, 2502, 2774, 2908
BstDSI CCRYGG 2 cut(s) 1480, 1853
BstF5I GGATG 7 cut(s) 133, 185, 650, 1056, 1421, 1488, 1610
BstFNI CGCG 2 cut(s) 2165, 3200
BstHHI GCGC 1 cut(s) 2167
BstMAI GTCTC 9 cut(s) 62, 483, 513, 574, 2251, 2278, 2753, 3145, 3195
BstNI CCWGG 2 cut(s) 775, 1136
BstNSI RCATGY 4 cut(s) 1582, 1681, 2656, 2878
BstSCI CCNGG 7 cut(s) 773, 1134, 2180, 2188, 2474, 2790, 3018
BstSFI CTRYAG 5 cut(s) 1169, 1510, 1529, 2226, 2974
BstSLI GKGCMC 1 cut(s) 1519
BstUI CGCG 2 cut(s) 2165, 3200
BstV1I GCAGC 9 cut(s) 210, 313, 1155, 1183, 1515, 1543, 2240, 2408, 2988
BstV2I GAAGAC 2 cut(s) 234, 3132
BstX2I RGATCY 1 cut(s) 307
BstXI CCANNNNNNTGG 5 cut(s) 401, 689, 1063, 1265, 1462
BstYI RGATCY 1 cut(s) 307
Bsu15I ATCGAT 1 cut(s) 3209
Bsu36I CCTNAGG 2 cut(s) 342, 3094
BsuI GTATCC 2 cut(s) 2156, 3268
BsuRI GGCC 4 cut(s) 838, 2127, 2325, 3205
BsuTUI ATCGAT 1 cut(s) 3209
BtgI CCRYGG 2 cut(s) 1480, 1853
BtsCI GGATG 7 cut(s) 133, 185, 650, 1056, 1421, 1488, 1610
BtsI GCAGTG 1 cut(s) 1519
BtsIMutI CAGTG 7 cut(s) 550, 1270, 1519, 2147, 2298, 2400, 3153
BtuMI TCGCGA 1 cut(s) 3200
Cac8I GCNNGC 8 cut(s) 24, 1536, 1580, 2027, 2129, 2502, 2774, 2908
CaiI CAGNNNCTG 2 cut(s) 871, 2402
CciI TCATGA 2 cut(s) 2280, 2683
CfoI GCGC 1 cut(s) 2167
Cfr10I RCCGGY 1 cut(s) 599
Cfr13I GGNCC 9 cut(s) 836, 859, 1124, 1197, 1378, 1596, 1633, 2063, 2126
ClaI ATCGAT 1 cut(s) 3209
CseI GACGC 1 cut(s) 2295
Csp6I GTAC 6 cut(s) 170, 1053, 1323, 1773, 2479, 2650
CspCI CAANNNNNGTGG 6 cut(s) 526, 561, 1764, 1799, 1842, 1877
CviQI GTAC 6 cut(s) 170, 1053, 1323, 1773, 2479, 2650
DraI TTTAAA 1 cut(s) 2722
DrdI GACNNNNNNGTC 1 cut(s) 2962
DriI GACNNNNNGTC 1 cut(s) 865
DseDI GACNNNNNNGTC 1 cut(s) 2962
EaeI YGGCCR 1 cut(s) 2323
Eam1104I CTCTTC 3 cut(s) 48, 1166, 3073
Eam1105I GACNNNNNGTC 1 cut(s) 865
EarI CTCTTC 3 cut(s) 48, 1166, 3073
Ecl136II GAGCTC 2 cut(s) 853, 1936
Eco130I CCWWGG 4 cut(s) 1493, 2623, 2658, 2668
Eco24I GRGCYC 2 cut(s) 855, 1938
Eco31I GGTCTC 3 cut(s) 483, 2251, 2753
Eco47I GGWCC 7 cut(s) 859, 1124, 1197, 1378, 1596, 1633, 2063
Eco53kI GAGCTC 2 cut(s) 853, 1936
Eco57I CTGAAG 4 cut(s) 317, 848, 1649, 1766
Eco81I CCTNAGG 2 cut(s) 342, 3094
Eco88I CYCGRG 2 cut(s) 256, 854
EcoICRI GAGCTC 2 cut(s) 853, 1936
EcoO109I RGGNCCY 4 cut(s) 859, 1124, 1596, 1633
EcoRI GAATTC 2 cut(s) 154, 1272
EcoRII CCWGG 2 cut(s) 773, 1134
EcoT14I CCWWGG 4 cut(s) 1493, 2623, 2658, 2668
EcoT22I ATGCAT 2 cut(s) 2941, 3088
EcoT38I GRGCYC 2 cut(s) 855, 1938
ErhI CCWWGG 4 cut(s) 1493, 2623, 2658, 2668
Esp3I CGTCTC 1 cut(s) 2278
FalI AAGNNNNNCTT 4 cut(s) 613, 645, 2451, 2483
FaqI GGGAC 6 cut(s) 872, 1210, 1479, 1609, 1619, 2807
FauI CCCGC 3 cut(s) 1187, 2136, 2234
FauNDI CATATG 2 cut(s) 940, 3147
FbaI TGATCA 2 cut(s) 450, 1305
FblI GTMKAC 2 cut(s) 2274, 2454
FokI GGATG 7 cut(s) 140, 192, 657, 1043, 1408, 1475, 1597
FriOI GRGCYC 2 cut(s) 855, 1938
GlaI GCGC 1 cut(s) 2166
GsuI CTGGAG 5 cut(s) 893, 1647, 2005, 3017, 3177
HaeIII GGCC 4 cut(s) 838, 2127, 2325, 3205
HapII CCGG 9 cut(s) 600, 978, 1917, 2181, 2190, 2475, 2791, 3009, 3020
HgaI GACGC 1 cut(s) 2295
HhaI GCGC 1 cut(s) 2167
Hin1I GRCGYC 1 cut(s) 2287
Hin6I GCGC 1 cut(s) 2165
HinP1I GCGC 1 cut(s) 2165
HincII GTYRAC 2 cut(s) 2178, 3117
HindII GTYRAC 2 cut(s) 2178, 3117
HindIII AAGCTT 7 cut(s) 20, 364, 428, 589, 1094, 1619, 1910
HpaI GTTAAC 1 cut(s) 3117
HpaII CCGG 9 cut(s) 600, 978, 1917, 2181, 2190, 2475, 2791, 3009, 3020
HphI GGTGA 9 cut(s) 261, 416, 535, 704, 748, 1351, 2612, 3064, 3073
Hpy166II GTNNAC 7 cut(s) 548, 1436, 2178, 2275, 2455, 2481, 3117
Hpy8I GTNNAC 7 cut(s) 548, 1436, 2178, 2275, 2455, 2481, 3117
Hpy99I CGWCG 1 cut(s) 2417
HpyCH4III ACNGT 7 cut(s) 359, 533, 1844, 2302, 2452, 2588, 3157
HpyCH4IV ACGT 1 cut(s) 2378
HpySE526I ACGT 1 cut(s) 2378
Hsp92I GRCGYC 1 cut(s) 2287
HspAI GCGC 1 cut(s) 2165
KpnI GGTACC 1 cut(s) 1056
Ksp22I TGATCA 2 cut(s) 450, 1305
KspAI GTTAAC 1 cut(s) 3117
LmnI GCTCC 6 cut(s) 1066, 1558, 1971, 2438, 2915, 2998
Lsp1109I GCAGC 9 cut(s) 210, 313, 1155, 1183, 1515, 1543, 2240, 2408, 2988
LweI GCATC 6 cut(s) 118, 1650, 2513, 3073, 3097, 3201
MaeII ACGT 1 cut(s) 2378
MaeIII GTNAC 8 cut(s) 523, 533, 1447, 2033, 2081, 2588, 2953, 3068
MbiI CCGCTC 1 cut(s) 2134
MfeI CAATTG 2 cut(s) 551, 945
MflI RGATCY 1 cut(s) 307
MhlI GDGCHC 3 cut(s) 855, 1519, 1938
MlsI TGGCCA 1 cut(s) 2325
MluNI TGGCCA 1 cut(s) 2325
MlyI GAGTC 2 cut(s) 1152, 3248
MmeI TCCRAC 2 cut(s) 1405, 1449
Mox20I TGGCCA 1 cut(s) 2325
Mph1103I ATGCAT 2 cut(s) 2941, 3088
MroXI GAANNNNTTC 3 cut(s) 1475, 2200, 2679
MscI TGGCCA 1 cut(s) 2325
MseI TTAA 6 cut(s) 1118, 1443, 2057, 2721, 3116, 3161
MslI CAYNNNNRTG 5 cut(s) 1061, 1263, 2090, 2951, 3185
Msp20I TGGCCA 1 cut(s) 2325
MspA1I CMGCKG 3 cut(s) 304, 1174, 2231
MspI CCGG 9 cut(s) 600, 978, 1917, 2181, 2190, 2475, 2791, 3009, 3020
MspR9I CCNGG 7 cut(s) 775, 1136, 2182, 2190, 2476, 2792, 3020
MunI CAATTG 2 cut(s) 551, 945
Mva1269I GAATGC 3 cut(s) 720, 1848, 2202
MvaI CCWGG 2 cut(s) 775, 1136
MvnI CGCG 2 cut(s) 2165, 3200
NciI CCSGG 5 cut(s) 2182, 2190, 2476, 2792, 3020
NdeI CATATG 2 cut(s) 940, 3147
NheI GCTAGC 1 cut(s) 1534
NmeAIII GCCGAG 1 cut(s) 2802
NmuCI GTSAC 3 cut(s) 523, 1447, 2953
NruI TCGCGA 1 cut(s) 3200
NsiI ATGCAT 2 cut(s) 2941, 3088
NspI RCATGY 4 cut(s) 1582, 1681, 2656, 2878
OliI CACNNNNGTG 1 cut(s) 3185
PaeI GCATGC 1 cut(s) 1582
PagI TCATGA 2 cut(s) 2280, 2683
PasI CCCWGGG 2 cut(s) 774, 1135
PciI ACATGT 1 cut(s) 1677
PctI GAATGC 3 cut(s) 720, 1848, 2202
PdmI GAANNNNTTC 3 cut(s) 1475, 2200, 2679
PflMI CCANNNNNTGG 1 cut(s) 1214
PleI GAGTC 2 cut(s) 1152, 3247
PpsI GAGTC 2 cut(s) 1152, 3247
PpuMI RGGWCCY 4 cut(s) 859, 1124, 1596, 1633
PscI ACATGT 1 cut(s) 1677
PsiI TTATAA 1 cut(s) 1905
Psp124BI GAGCTC 2 cut(s) 855, 1938
Psp5II RGGWCCY 4 cut(s) 859, 1124, 1596, 1633
Psp6I CCWGG 2 cut(s) 773, 1134
PspGI CCWGG 2 cut(s) 773, 1134
PspPI GGNCC 9 cut(s) 836, 859, 1124, 1197, 1378, 1596, 1633, 2063, 2126
PspPPI RGGWCCY 4 cut(s) 859, 1124, 1596, 1633
PstI CTGCAG 4 cut(s) 1173, 1514, 1533, 2230
PstNI CAGNNNCTG 2 cut(s) 871, 2402
PsuI RGATCY 1 cut(s) 307
PvuII CAGCTG 3 cut(s) 304, 1174, 2231
RruI TCGCGA 1 cut(s) 3200
RsaI GTAC 6 cut(s) 171, 1054, 1324, 1774, 2480, 2651
RsaNI GTAC 6 cut(s) 170, 1053, 1323, 1773, 2479, 2650
RseI CAYNNNNRTG 5 cut(s) 1061, 1263, 2090, 2951, 3185
SacI GAGCTC 2 cut(s) 855, 1938
SaqAI TTAA 6 cut(s) 1118, 1443, 2057, 2721, 3116, 3161
Sau96I GGNCC 9 cut(s) 836, 859, 1124, 1197, 1378, 1596, 1633, 2063, 2126
SchI GAGTC 2 cut(s) 1152, 3248
ScrFI CCNGG 7 cut(s) 775, 1136, 2182, 2190, 2476, 2792, 3020
SduI GDGCHC 3 cut(s) 855, 1519, 1938
SfaNI GCATC 6 cut(s) 118, 1650, 2513, 3073, 3097, 3201
SfcI CTRYAG 5 cut(s) 1169, 1510, 1529, 2226, 2974
SinI GGWCC 7 cut(s) 859, 1124, 1197, 1378, 1596, 1633, 2063
SmiMI CAYNNNNRTG 5 cut(s) 1061, 1263, 2090, 2951, 3185
SmlI CTYRAG 6 cut(s) 575, 719, 1016, 1736, 1955, 2316
SmoI CTYRAG 6 cut(s) 575, 719, 1016, 1736, 1955, 2316
SphI GCATGC 1 cut(s) 1582
SsiI CCGC 8 cut(s) 1194, 2124, 2129, 2132, 2241, 2462, 2520, 2738
SstI GAGCTC 2 cut(s) 855, 1938
StyD4I CCNGG 7 cut(s) 773, 1134, 2180, 2188, 2474, 2790, 3018
StyI CCWWGG 4 cut(s) 1493, 2623, 2658, 2668
TaaI ACNGT 7 cut(s) 359, 533, 1844, 2302, 2452, 2588, 3157
TaiI ACGT 1 cut(s) 2381
TaqI TCGA 6 cut(s) 209, 245, 1458, 2204, 2412, 3209
TaqII GACCGA 1 cut(s) 1826
TatI WGTACW 1 cut(s) 2649
TauI GCSGC 4 cut(s) 2127, 2134, 2523, 2741
Tru1I TTAA 6 cut(s) 1118, 1443, 2057, 2721, 3116, 3161
Tru9I TTAA 6 cut(s) 1118, 1443, 2057, 2721, 3116, 3161
TscAI CASTG 7 cut(s) 550, 1270, 1519, 2147, 2298, 2407, 3160
TseFI GTSAC 3 cut(s) 523, 1447, 2953
TseI GCWGC 9 cut(s) 198, 301, 1168, 1171, 1528, 1531, 2228, 2396, 3001
Tsp45I GTSAC 3 cut(s) 523, 1447, 2953
TspGWI ACGGA 2 cut(s) 1469, 2069
TspRI CASTG 7 cut(s) 550, 1270, 1519, 2147, 2298, 2407, 3160
Van91I CCANNNNNTGG 1 cut(s) 1214
VpaK11BI GGWCC 7 cut(s) 859, 1124, 1197, 1378, 1596, 1633, 2063
XapI RAATTY 4 cut(s) 139, 154, 336, 1272
XceI RCATGY 4 cut(s) 1582, 1681, 2656, 2878
XcmI CCANNNNNNNNNTGG 1 cut(s) 1215
XmaJI CCTAGG 1 cut(s) 1493
XmiI GTMKAC 2 cut(s) 2274, 2454
XmnI GAANNNNTTC 3 cut(s) 1475, 2200, 2679
Zsp2I ATGCAT 2 cut(s) 2941, 3088
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.