MD12G1253000.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0011826
Physical Location & Seq
Forward (+)
32192999 .. 32196402
3404 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1253000.v1.1.491

Sequence Viewer

Length: 3207 bp
ATGGTTATGGTGAACATGTCATATGGTTTCTTGATTACTATAGTTGTCCTTGTTGCTGCCCTTTTGGTTTTCGAATCCGAGGGGCTTAACGATGAGGGTCAATATCTTCTAAAATTCAAGAGCGGATTAGTTGATAGGTTTGATCATCTTGGTAGTTGGAATTCCAATGATTTCACACCTTGTGGATGGAGAGGAGTGAATTGCAGCAGTGGATACAATCCACTTGTGCTGTCTCTTGACTTGAGCTCAATGAATCTTTCGGGTTATTTGTCTCCGAGCATTGGCGGATTGGTTCACCTGAACTATCTTAATCTTTCTTTCAATGAACTGACTCGGAGTATACCTGAAGACATCGGAAATTGTTCGAGTTTGGAGGTTCTAAATTTGAATGACAATAACTTTATAGGTCAAATTCCTAAACCAATAGCTACGCTTTCAAGTTTACGAGTACTGAATCTCTGCAACAACAGATTATCAGGGCCTATCCATGAAGAAATTGGAAATCTTTCATCCCTGTCCCAGTTGATTGCCTACTCTAACAATCTTAGCGGAATTTTGCCGCGCTCTCTTGGAAATCTCAAAAGATTGAGGACTTTCCGGGCAGGGGAGAACTTAATATCCGGAAGCTTGCCTACAGAGATAGGTGCATGTGAGAGCTTGGAGGTTCTTGGTCTAGCGGAAAATCAGTTAAGTGAAGAAATGCCTAAGGAGCTCGGAATGCTTGAGAATTTGGGAGCTTTGTTTCTTTGGGGTAACCAGCTTTCCGGGGTTATTCCAAAGGAGCTTGGTAACTGTACAAATTTGGAAATTCTTGCCCTGTATGAGAACAAGCTTGTGGGAGAGATTCCAAGGGAGCTCGGAAACATTGCTCATCTGGAGAAGTTGTATCTTTACAGAAATATGTTGAATGGGACAGTTCCAAGAGAGATTGGCAATCTTTCTCTAGTAACACAGATTGATCTTTCGGAGAACTTGTTATCAGGAGAGATACCGGTTGAGCTCAGTAAGATAGCGGCATTGCAATTGCTTTATCTTTTCAAGAACCAATTCACAGGTGTTATTCCAGATGAGTTCACAAGCCTTACAAACCTGACTAAGTTCGACCTCTCTATCAATTTTCTCACCGGTTCAATTCCTATCGGAATCCAGTACTTGACTGAACTGATCATGCTGCAATTGTTCCACAATTTGCTGAGTGGTGTAATCCCTCAAGGCCTTGGAGTGTATAGCGCGCTTTGGGTGGTTGACTTATCCGAAAACTTCCTAACAGGGGGAGTTCCTCATCATCTCTGCAGAAATTCTAACATGATTTTGTTAAATTTGGGGTCTAACAGGCTCACTGGGAATATACCAAATGGCATCACAAGCTGCAAGTCATTAGTGCAGCTTCGTCTAGTTGGAAACAACCTCACGGGTACTTTTCCATCCGAAATGTGCAAACTGGTTAACCTTTCTACAATGGAGTTGGATCAGAACAAGTTCAGGGGAGCCATTCCTCCAGAAATTGGCAACTGTAGAACTTTGCAAAGGCTTCACCTTTCGGGCAACTACTTCACATCGGAGCTGCCTAGAGAGATTGGTAGCCTCCCACAGTTGGTGACCTTTAATGTCTCATCCAATTCTCTTTCTGGAAGGATACCGCCTGAAATTTTCAATTGTAAAACACTTCAACGGCTTGATCTTAGCAATAACAACTTTTCAGATGCATTGCCAAGTGAGATAGGAACACTTTCTCAGTTGGAGCTTCTCAAGCTTTCTGAAAACAATCTTTCCGGAAACATACCGGTTGCGCTGGGAAACCTTGTACGTTTGACTGAGCTACAAATGGGTGGCAACTCATTCTCTGGCGGCATCCCAGCGGAGTTGGGTTCCCTTTCGAGCCTGCAGATTGCATTAAATCTCAGTTTTAACAACCTTTCCGGGCAGATACCTCCTCAGCTTGGCAATCTTATCTTACTGGAATTCCTGCTACTCAATAACAACAATTTGACAGGTACAATTCCAGGTGTTTTCAAGAATCTTTTGAGTCTACTTGTCTGCAACCTGTCGTACAATGCCTTGGTAGGGCCTATTCCTCATTTACCATTCTTTCAGAATATGACCGTTGACAGCTTTGTTGGGAATAAAGGACTCTGCGGCGGGCCTCTTGGTGATTGCGCAACATCACCATATTCACTCTCTTTTCCTCCGGATATGGCGAAGAAACGCCCCCACTTGGGTAAAATCATCGCTATCATCGCAGCAGCTATTGGTGGTGTTTCTCTCATTTTGATTGCAATCCTTGTATATGTCATGAGACGTCCCACGAGCGCTGCAGGGTTCACTTTCCAAGACTTGGTTGCGGCGACTGAAAATATTGATGAGAGCTTTGCAATTGGAAGAGGAGCTTGTGGAACCGTGTACAAAGCAGTCTTACCGACGGGTCATACAGTTGCTGTTAAGAAGGTAGTATCCAGCACGGAGGGGAACAATGTGGATAACAGTTTTCATGCTGAAATTCTAACTTTAGGAAAAATCAGGCATCGAAATATTGTAAAGCTATGCGGTTTCTGCTACTACCAGGGTTGCAATCTTCTGCTTTACGAGTACATGGCGAGAGGCAGCTTGGGAAAATTGCTTCAAGGAACATCTTGTAGTCTTGATTGGACAACGAGATTCATGATTGCTCTTGGGGCTGCTCAGGGTCGCTCTTACATGCATCATGATTGCAAACCGATAATCTTTCACTGTGATATAAAGTCCAATAACATTCTTCTTGACGACAAGGTTGAATCTCGTGTTGGGGATTTCGGGTTGGCAAAGGTGATTGACATGCCACACTCTACGACTATGCCTGTAGTTGCAGGTTCTTATGGATATGTTGCTCCTGATTATGCATACACTTTGAAAGTCACAGAAAAATGTGACATCTACAGCTTCGGAGTAGTCCTTCTGGAGTTGCTGACCGGCAGAACGCCTGTACAATCGATAGAGGAAGGCGGTGACCTGGTAACATGGGTAAGAAACTATTTTCTGCACCATTCGTTGTCTTCAGGAGTACTCGATGCTCGATTAGATTTACAAGATGAAGCTACTGTCTCTCACATGATTACTGTCTTGAAAATTGCTTTGACGTGCACAAGTATGTCCCCTTTCGACCGCCCGACCATGAGAGAAGTTGTTTCCATGCTCATTGGGTCAAATGAGAGGAGAAAGGACACACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

1069

Amino Acids

116.33

Weight (kDa)

5.74

Isoelectric Point (pI)

34.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 29 - 69 6.9e-12 Leucine rich repeat N-terminal domain
LRR_14 PF23598 79 - 200 1.1e-13 Leucine-rich repeat region
LRR_4 PF12799 99 - 139 3.3e-06 Leucine Rich repeats (2 copies)
LRR_14 PF23598 188 - 297 1.3e-08 Leucine-rich repeat region
LRR_14 PF23598 477 - 561 7e-07 Leucine-rich repeat region
LRR_14 PF23598 545 - 630 5.6e-09 Leucine-rich repeat region
LRR_8 PF13855 555 - 613 3e-07 Leucine rich repeat
Pkinase PF00069 791 - 1055 3.6e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 791 - 1057 7.5e-36 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011826)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G63930 AT5G63930
fragaria_vesca FvH4_6g01320 FvH4_6g01320
malus_domestica MD04G1235000.v1.1 MD12G1253000.v1.1
prunus_persica Prupe.6G354900_v2.0.a1 Prupe.6G354900_v2.0.a1
pyrus_communis pycom04g20870 pycom12g23210
rosa_chinensis RchiOBHm_Chr3g0448891
rosa_laevigata RLG00000025869
rosa_multiflora Rmu_co8340729.1_g000001 Rmu_sc0003116.1_g000020
rosa_rugosa Rorug02G0617000
rosa_samantha Rh3AG015200 Rh3CG013900 Rh3DG015500
rosa_wichuraiana Rw3G001130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2302
Acc16I TGCGCA 1 cut(s) 2158
Acc36I ACCTGC 1 cut(s) 2834
AccB7I CCANNNNNTGG 2 cut(s) 1505, 2335
AccBSI CCGCTC 1 cut(s) 123
AccI GTMKAC 2 cut(s) 340, 2029
AccII CGCG 2 cut(s) 562, 1232
AccIII TCCGGA 3 cut(s) 620, 1772, 2188
AclWI GGATC 1 cut(s) 1476
AcuI CTGAAG 2 cut(s) 366, 3015
AcyI GRCGYC 1 cut(s) 2299
AdeI CACNNNGTG 1 cut(s) 182
AfeI AGCGCT 1 cut(s) 2311
AflIII ACRYGT 1 cut(s) 15
AgeI ACCGGT 3 cut(s) 991, 1124, 1783
AjiI CACGTC 1 cut(s) 3114
AjnI CCWGG 3 cut(s) 2002, 2559, 2985
AjuI GAANNNNNNNTTGG 2 cut(s) 2763, 2795
AloI GAACNNNNNNTCC 2 cut(s) 602, 634
Alw21I GWGCWC 5 cut(s) 248, 714, 858, 1002, 3119
Alw26I GTCTC 5 cut(s) 237, 276, 1615, 2290, 3082
Alw44I GTGCAC 1 cut(s) 3115
AlwI GGATC 1 cut(s) 1476
AlwNI CAGNNNCTG 1 cut(s) 2435
Aor13HI TCCGGA 3 cut(s) 620, 1772, 2188
Aor51HI AGCGCT 1 cut(s) 2311
AoxI GGCC 4 cut(s) 479, 1213, 2066, 2141
ApaLI GTGCAC 1 cut(s) 3115
AsiGI ACCGGT 3 cut(s) 991, 1124, 1783
Asp700I GAANNNNTTC 5 cut(s) 361, 505, 1046, 1478, 1729
AspLEI GCGC 6 cut(s) 564, 1232, 1234, 1792, 2159, 2312
AspS9I GGNCC 3 cut(s) 479, 2066, 2141
AsuC2I CCSGG 3 cut(s) 599, 766, 1921
AsuHPI GGTGA 9 cut(s) 22, 287, 1114, 1526, 1609, 2157, 2162, 2815, 2993
AsuII TTCGAA 1 cut(s) 72
AxyI CCTNAGG 1 cut(s) 705
BaeGI GKGCMC 1 cut(s) 3119
BanII GRGCYC 4 cut(s) 248, 714, 858, 1002
BarI GAAGNNNNNNTAC 2 cut(s) 616, 648
BauI CACGAG 2 cut(s) 2305, 2775
BbsI GAAGAC 2 cut(s) 354, 3021
Bbv12I GWGCWC 5 cut(s) 248, 714, 858, 1002, 3119
BbvCI CCTCAGC 1 cut(s) 1935
BccI CCATC 2 cut(s) 180, 1432
BceAI ACGGC 1 cut(s) 1688
BciT130I CCWGG 3 cut(s) 2004, 2561, 2987
BciVI GTATCC 3 cut(s) 206, 1629, 2461
BclI TGATCA 2 cut(s) 142, 1164
BcnI CCSGG 3 cut(s) 599, 766, 1921
BcoDI GTCTC 5 cut(s) 237, 276, 1615, 2290, 3082
BfaI CTAG 4 cut(s) 674, 944, 1394, 1569
BfmI CTRYAG 8 cut(s) 39, 633, 1291, 1513, 1883, 2313, 2835, 2911
BfoI RGCGCY 1 cut(s) 2313
BfuAI ACCTGC 1 cut(s) 2834
BfuI GTATCC 3 cut(s) 206, 1629, 2461
BmcAI AGTACT 3 cut(s) 450, 1151, 3039
Bme1390I CCNGG 6 cut(s) 599, 766, 1921, 2004, 2561, 2987
BmgBI CACGTC 1 cut(s) 3114
BmgT120I GGNCC 3 cut(s) 479, 2066, 2141
BmiI GGNNCC 3 cut(s) 1489, 1870, 2395
BmrFI CCNGG 6 cut(s) 599, 766, 1921, 2004, 2561, 2987
BmrI ACTGGG 2 cut(s) 514, 1350
BmsI GCATC 6 cut(s) 1368, 1693, 1860, 2530, 2707, 3034
BmuI ACTGGG 2 cut(s) 514, 1350
BpiI GAAGAC 2 cut(s) 354, 3021
BpmI CTGGAG 3 cut(s) 896, 1482, 2954
Bpu10I CCTNAGC 2 cut(s) 1935, 2679
Bpu14I TTCGAA 1 cut(s) 72
BpuEI CTTGAG 4 cut(s) 262, 743, 1194, 1733
BpuMI CCSGG 3 cut(s) 599, 766, 1921
Bsa29I ATCGAT 1 cut(s) 2966
BsaBI GATNNNNATC 1 cut(s) 2276
BsaHI GRCGYC 1 cut(s) 2299
BsaJI CCNNGG 6 cut(s) 78, 765, 848, 1216, 2058, 2560
BsaWI WCCGGW 6 cut(s) 620, 991, 1124, 1772, 1783, 2188
BsaXI ACNNNNNCTCC 4 cut(s) 869, 899, 959, 989
Bse118I RCCGGY 4 cut(s) 991, 1124, 1783, 2945
Bse1I ACTGG 5 cut(s) 520, 1147, 1345, 1446, 1962
Bse21I CCTNAGG 1 cut(s) 705
Bse3DI GCAATG 3 cut(s) 864, 1016, 1706
Bse8I GATNNNNATC 1 cut(s) 2276
BseAI TCCGGA 3 cut(s) 620, 1772, 2188
BseBI CCWGG 3 cut(s) 2004, 2561, 2987
BseCI ATCGAT 1 cut(s) 2966
BseDI CCNNGG 6 cut(s) 78, 765, 848, 1216, 2058, 2560
BseGI GGATG 5 cut(s) 191, 509, 1424, 1613, 1851
BseJI GATNNNNATC 1 cut(s) 2276
BseMI GCAATG 3 cut(s) 864, 1016, 1706
BseMII CTCAG 7 cut(s) 1015, 1184, 1748, 1806, 1915, 1949, 2693
BseNI ACTGG 5 cut(s) 520, 1147, 1345, 1446, 1962
BsePI GCGCGC 1 cut(s) 1230
BseRI GAGGAG 4 cut(s) 207, 1923, 2397, 3202
BseSI GKGCMC 1 cut(s) 3119
BseYI CCCAGC 2 cut(s) 1792, 1855
BsgI GTGCAG 2 cut(s) 1403, 2999
Bsh1236I CGCG 2 cut(s) 562, 1232
Bsh1285I CGRYCG 1 cut(s) 3139
BshFI GGCC 4 cut(s) 481, 1215, 2068, 2143
BshTI ACCGGT 3 cut(s) 991, 1124, 1783
BshVI ATCGAT 1 cut(s) 2966
BsiEI CGRYCG 1 cut(s) 3139
BsiHKAI GWGCWC 5 cut(s) 248, 714, 858, 1002, 3119
BslFI GGGAC 4 cut(s) 502, 925, 2286, 3112
BsmAI GTCTC 5 cut(s) 237, 276, 1615, 2290, 3082
BsmBI CGTCTC 1 cut(s) 2290
BsmFI GGGAC 4 cut(s) 502, 925, 2286, 3112
BsmI GAATGC 1 cut(s) 723
BsnI GGCC 4 cut(s) 481, 1215, 2068, 2143
Bsp119I TTCGAA 1 cut(s) 72
Bsp1286I GDGCHC 5 cut(s) 248, 714, 858, 1002, 3119
Bsp13I TCCGGA 3 cut(s) 620, 1772, 2188
Bsp1407I TGTACA 3 cut(s) 794, 2400, 2959
Bsp143I GATC 5 cut(s) 142, 958, 1164, 1468, 1678
BspANI GGCC 4 cut(s) 481, 1215, 2068, 2143
BspCNI CTCAG 7 cut(s) 1014, 1185, 1747, 1807, 1914, 1948, 2692
BspDI ATCGAT 1 cut(s) 2966
BspEI TCCGGA 3 cut(s) 620, 1772, 2188
BspFNI CGCG 2 cut(s) 562, 1232
BspHI TCATGA 3 cut(s) 2292, 2658, 2701
BspLI GGNNCC 3 cut(s) 1489, 1870, 2395
BspMAI CTGCAG 3 cut(s) 1295, 1887, 2317
BspMI ACCTGC 1 cut(s) 2834
BspPI GGATC 1 cut(s) 1476
BspT104I TTCGAA 1 cut(s) 72
BsrBI CCGCTC 1 cut(s) 123
BsrDI GCAATG 3 cut(s) 864, 1016, 1706
BsrFI RCCGGY 4 cut(s) 991, 1124, 1783, 2945
BsrGI TGTACA 3 cut(s) 794, 2400, 2959
BsrI ACTGG 5 cut(s) 520, 1147, 1345, 1446, 1962
BssAI RCCGGY 4 cut(s) 991, 1124, 1783, 2945
BssECI CCNNGG 6 cut(s) 78, 765, 848, 1216, 2058, 2560
BssHII GCGCGC 1 cut(s) 1230
BssMI GATC 5 cut(s) 142, 958, 1164, 1468, 1678
BssNAI GTATAC 1 cut(s) 341
BssNI GRCGYC 1 cut(s) 2299
BssSI CACGAG 2 cut(s) 2305, 2775
BssT1I CCWWGG 3 cut(s) 848, 1216, 2058
Bst1107I GTATAC 1 cut(s) 341
Bst2BI CACGAG 2 cut(s) 2305, 2775
Bst2UI CCWGG 3 cut(s) 2004, 2561, 2987
Bst6I CTCTTC 1 cut(s) 2374
BstACI GRCGYC 1 cut(s) 2299
BstAUI TGTACA 3 cut(s) 794, 2400, 2959
BstBI TTCGAA 1 cut(s) 72
BstC8I GCNNGC 4 cut(s) 629, 1232, 1883, 2141
BstEII GGTNACC 3 cut(s) 752, 1597, 2981
BstF5I GGATG 5 cut(s) 191, 509, 1424, 1613, 1851
BstFNI CGCG 2 cut(s) 562, 1232
BstH2I RGCGCY 1 cut(s) 2313
BstHHI GCGC 6 cut(s) 564, 1232, 1234, 1792, 2159, 2312
BstKTI GATC 5 cut(s) 145, 961, 1167, 1471, 1681
BstMAI GTCTC 5 cut(s) 237, 276, 1615, 2290, 3082
BstMBI GATC 5 cut(s) 142, 958, 1164, 1468, 1678
BstMCI CGRYCG 1 cut(s) 3139
BstMWI GCNNNNNNNGC 7 cut(s) 709, 718, 1365, 1750, 2237, 2550, 2672
BstNI CCWGG 3 cut(s) 2004, 2561, 2987
BstNSI RCATGY 4 cut(s) 19, 651, 2698, 2815
BstPI GGTNACC 3 cut(s) 752, 1597, 2981
BstSCI CCNGG 6 cut(s) 597, 764, 1919, 2002, 2559, 2985
BstSFI CTRYAG 8 cut(s) 39, 633, 1291, 1513, 1883, 2313, 2835, 2911
BstSLI GKGCMC 1 cut(s) 3119
BstUI CGCG 2 cut(s) 562, 1232
BstV2I GAAGAC 2 cut(s) 354, 3021
BstZ17I GTATAC 1 cut(s) 341
Bsu15I ATCGAT 1 cut(s) 2966
Bsu36I CCTNAGG 1 cut(s) 705
BsuI GTATCC 3 cut(s) 206, 1629, 2461
BsuRI GGCC 4 cut(s) 481, 1215, 2068, 2143
BsuTUI ATCGAT 1 cut(s) 2966
BtgZI GCGATG 2 cut(s) 2212, 2221
BtrI CACGTC 1 cut(s) 3114
BtsCI GGATG 5 cut(s) 191, 509, 1424, 1613, 1851
BtsI GCAGTG 1 cut(s) 214
BtsIMutI CAGTG 3 cut(s) 214, 1338, 2725
BveI ACCTGC 1 cut(s) 2834
Cac8I GCNNGC 4 cut(s) 629, 1232, 1883, 2141
CaiI CAGNNNCTG 1 cut(s) 2435
CciI TCATGA 3 cut(s) 2292, 2658, 2701
CfoI GCGC 6 cut(s) 564, 1232, 1234, 1792, 2159, 2312
Cfr10I RCCGGY 4 cut(s) 991, 1124, 1783, 2945
Cfr13I GGNCC 3 cut(s) 479, 2066, 2141
ClaI ATCGAT 1 cut(s) 2966
CsiI ACCWGGT 1 cut(s) 2985
CspAI ACCGGT 3 cut(s) 991, 1124, 1783
DpnI GATC 5 cut(s) 144, 960, 1166, 1470, 1680
DpnII GATC 5 cut(s) 142, 958, 1164, 1468, 1678
DraIII CACNNNGTG 1 cut(s) 182
Eam1104I CTCTTC 1 cut(s) 2374
EarI CTCTTC 1 cut(s) 2374
EciI GGCGGA 1 cut(s) 300
Ecl136II GAGCTC 4 cut(s) 246, 712, 856, 1000
Eco130I CCWWGG 3 cut(s) 848, 1216, 2058
Eco147I AGGCCT 1 cut(s) 1215
Eco24I GRGCYC 4 cut(s) 248, 714, 858, 1002
Eco47III AGCGCT 1 cut(s) 2311
Eco53kI GAGCTC 4 cut(s) 246, 712, 856, 1000
Eco57I CTGAAG 2 cut(s) 366, 3015
Eco81I CCTNAGG 1 cut(s) 705
Eco91I GGTNACC 3 cut(s) 752, 1597, 2981
EcoICRI GAGCTC 4 cut(s) 246, 712, 856, 1000
EcoO109I RGGNCCY 2 cut(s) 479, 2066
EcoO65I GGTNACC 3 cut(s) 752, 1597, 2981
EcoRI GAATTC 2 cut(s) 160, 1961
EcoRII CCWGG 3 cut(s) 2002, 2559, 2985
EcoT14I CCWWGG 3 cut(s) 848, 1216, 2058
EcoT22I ATGCAT 3 cut(s) 1708, 2700, 2878
EcoT38I GRGCYC 4 cut(s) 248, 714, 858, 1002
ErhI CCWWGG 3 cut(s) 848, 1216, 2058
Esp3I CGTCTC 1 cut(s) 2290
FalI AAGNNNNNCTT 2 cut(s) 2371, 2403
FaqI GGGAC 4 cut(s) 502, 925, 2286, 3112
FauI CCCGC 1 cut(s) 2132
FauNDI CATATG 1 cut(s) 22
FbaI TGATCA 2 cut(s) 142, 1164
FblI GTMKAC 2 cut(s) 340, 2029
FokI GGATG 5 cut(s) 198, 496, 1411, 1600, 1838
FriOI GRGCYC 4 cut(s) 248, 714, 858, 1002
FspBI CTAG 4 cut(s) 674, 944, 1394, 1569
FspI TGCGCA 1 cut(s) 2158
GlaI GCGC 6 cut(s) 563, 1231, 1233, 1791, 2158, 2311
GsaI CCCAGC 2 cut(s) 1796, 1859
GsuI CTGGAG 3 cut(s) 896, 1482, 2954
HaeII RGCGCY 1 cut(s) 2313
HaeIII GGCC 4 cut(s) 481, 1215, 2068, 2143
HhaI GCGC 6 cut(s) 564, 1232, 1234, 1792, 2159, 2312
Hin1I GRCGYC 1 cut(s) 2299
Hin6I GCGC 6 cut(s) 562, 1230, 1232, 1790, 2157, 2310
HinP1I GCGC 6 cut(s) 562, 1230, 1232, 1790, 2157, 2310
HincII GTYRAC 3 cut(s) 1246, 1447, 2107
HindII GTYRAC 3 cut(s) 1246, 1447, 2107
HindIII AAGCTT 3 cut(s) 625, 830, 1751
HpaI GTTAAC 1 cut(s) 1447
HphI GGTGA 9 cut(s) 22, 287, 1114, 1526, 1609, 2157, 2162, 2815, 2993
Hpy99I CGWCG 1 cut(s) 2422
HpyAV CCTTC 4 cut(s) 1626, 2437, 2939, 2969
HpyCH4IV ACGT 3 cut(s) 1807, 2299, 3113
HpyF10VI GCNNNNNNNGC 7 cut(s) 709, 718, 1365, 1750, 2237, 2550, 2672
HpySE526I ACGT 3 cut(s) 1807, 2299, 3113
Hsp92I GRCGYC 1 cut(s) 2299
HspAI GCGC 6 cut(s) 562, 1230, 1232, 1790, 2157, 2310
Kpn2I TCCGGA 3 cut(s) 620, 1772, 2188
Ksp22I TGATCA 2 cut(s) 142, 1164
KspAI GTTAAC 1 cut(s) 1447
Kzo9I GATC 5 cut(s) 142, 958, 1164, 1468, 1678
LmnI GCTCC 9 cut(s) 709, 734, 781, 853, 1487, 1561, 1741, 2384, 2869
LweI GCATC 6 cut(s) 1368, 1693, 1860, 2530, 2707, 3034
MabI ACCWGGT 1 cut(s) 2985
MaeI CTAG 4 cut(s) 674, 944, 1394, 1569
MaeII ACGT 3 cut(s) 1807, 2299, 3113
MaeIII GTNAC 8 cut(s) 752, 788, 946, 1597, 2890, 2903, 2981, 2989
MalI GATC 5 cut(s) 144, 960, 1166, 1470, 1680
MbiI CCGCTC 1 cut(s) 123
MboI GATC 5 cut(s) 142, 958, 1164, 1468, 1678
MboII GAAGA 9 cut(s) 98, 359, 503, 707, 2212, 2391, 2564, 2744, 3021
MfeI CAATTG 4 cut(s) 1022, 1175, 1654, 2373
MhlI GDGCHC 5 cut(s) 248, 714, 858, 1002, 3119
MlyI GAGTC 3 cut(s) 325, 2035, 2124
MmeI TCCRAC 4 cut(s) 137, 1378, 1446, 1719
Mph1103I ATGCAT 3 cut(s) 1708, 2700, 2878
MroI TCCGGA 3 cut(s) 620, 1772, 2188
MroXI GAANNNNTTC 5 cut(s) 361, 505, 1046, 1478, 1729
MslI CAYNNNNRTG 1 cut(s) 3122
MspA1I CMGCKG 1 cut(s) 1859
MspR9I CCNGG 6 cut(s) 599, 766, 1921, 2004, 2561, 2987
MunI CAATTG 4 cut(s) 1022, 1175, 1654, 2373
Mva1269I GAATGC 1 cut(s) 723
MvaI CCWGG 3 cut(s) 2004, 2561, 2987
MvnI CGCG 2 cut(s) 562, 1232
MwoI GCNNNNNNNGC 7 cut(s) 709, 718, 1365, 1750, 2237, 2550, 2672
NciI CCSGG 3 cut(s) 599, 766, 1921
NdeI CATATG 1 cut(s) 22
NdeII GATC 5 cut(s) 142, 958, 1164, 1468, 1678
NlaIV GGNNCC 3 cut(s) 1489, 1870, 2395
NmuCI GTSAC 4 cut(s) 1597, 2890, 2903, 2981
NsbI TGCGCA 1 cut(s) 2158
NsiI ATGCAT 3 cut(s) 1708, 2700, 2878
NspI RCATGY 4 cut(s) 19, 651, 2698, 2815
NspV TTCGAA 1 cut(s) 72
PagI TCATGA 3 cut(s) 2292, 2658, 2701
PauI GCGCGC 1 cut(s) 1230
PceI AGGCCT 1 cut(s) 1215
PciI ACATGT 1 cut(s) 15
PctI GAATGC 1 cut(s) 723
PdmI GAANNNNTTC 5 cut(s) 361, 505, 1046, 1478, 1729
PfeI GAWTC 8 cut(s) 74, 253, 454, 844, 1143, 2017, 2655, 2771
PflMI CCANNNNNTGG 2 cut(s) 1505, 2335
PinAI ACCGGT 3 cut(s) 991, 1124, 1783
PleI GAGTC 3 cut(s) 325, 2034, 2124
PpsI GAGTC 3 cut(s) 325, 2034, 2124
PscI ACATGT 1 cut(s) 15
Psp124BI GAGCTC 4 cut(s) 248, 714, 858, 1002
Psp6I CCWGG 3 cut(s) 2002, 2559, 2985
PspEI GGTNACC 3 cut(s) 752, 1597, 2981
PspFI CCCAGC 2 cut(s) 1792, 1855
PspGI CCWGG 3 cut(s) 2002, 2559, 2985
PspN4I GGNNCC 3 cut(s) 1489, 1870, 2395
PspPI GGNCC 3 cut(s) 479, 2066, 2141
PstI CTGCAG 3 cut(s) 1295, 1887, 2317
PstNI CAGNNNCTG 1 cut(s) 2435
PteI GCGCGC 1 cut(s) 1230
RseI CAYNNNNRTG 1 cut(s) 3122
SacI GAGCTC 4 cut(s) 248, 714, 858, 1002
Sau3AI GATC 5 cut(s) 142, 958, 1164, 1468, 1678
Sau96I GGNCC 3 cut(s) 479, 2066, 2141
ScaI AGTACT 3 cut(s) 450, 1151, 3039
SchI GAGTC 3 cut(s) 325, 2035, 2124
ScrFI CCNGG 6 cut(s) 599, 766, 1921, 2004, 2561, 2987
SduI GDGCHC 5 cut(s) 248, 714, 858, 1002, 3119
SexAI ACCWGGT 1 cut(s) 2985
SfaNI GCATC 6 cut(s) 1368, 1693, 1860, 2530, 2707, 3034
SfcI CTRYAG 8 cut(s) 39, 633, 1291, 1513, 1883, 2313, 2835, 2911
SfuI TTCGAA 1 cut(s) 72
SmiMI CAYNNNNRTG 1 cut(s) 3122
SmlI CTYRAG 4 cut(s) 241, 722, 1209, 1748
SmoI CTYRAG 4 cut(s) 241, 722, 1209, 1748
SseBI AGGCCT 1 cut(s) 1215
SspI AATATT 2 cut(s) 2356, 2530
SspMI CTAG 4 cut(s) 674, 944, 1394, 1569
SstI GAGCTC 4 cut(s) 248, 714, 858, 1002
StuI AGGCCT 1 cut(s) 1215
StyD4I CCNGG 6 cut(s) 597, 764, 1919, 2002, 2559, 2985
StyI CCWWGG 3 cut(s) 848, 1216, 2058
TaiI ACGT 3 cut(s) 1810, 2302, 3116
TaqI TCGA 9 cut(s) 72, 365, 1101, 1877, 2524, 2966, 3042, 3049, 3135
TatI WGTACW 7 cut(s) 448, 794, 1149, 2400, 2586, 2959, 3037
TauI GCSGC 5 cut(s) 562, 1016, 1851, 2139, 2345
TfiI GAWTC 8 cut(s) 74, 253, 454, 844, 1143, 2017, 2655, 2771
TscAI CASTG 3 cut(s) 214, 1345, 2732
TseFI GTSAC 4 cut(s) 1597, 2890, 2903, 2981
Tsp45I GTSAC 4 cut(s) 1597, 2890, 2903, 2981
TspDTI ATGAA 7 cut(s) 266, 339, 498, 504, 2477, 2647, 3081
TspGWI ACGGA 1 cut(s) 2474
TspRI CASTG 3 cut(s) 214, 1345, 2732
Van91I CCANNNNNTGG 2 cut(s) 1505, 2335
VneI GTGCAC 1 cut(s) 3115
XceI RCATGY 4 cut(s) 19, 651, 2698, 2815
XcmI CCANNNNNNNNNTGG 1 cut(s) 494
XmiI GTMKAC 2 cut(s) 340, 2029
XmnI GAANNNNTTC 5 cut(s) 361, 505, 1046, 1478, 1729
XspI CTAG 4 cut(s) 674, 944, 1394, 1569
ZraI GACGTC 1 cut(s) 2300
ZrmI AGTACT 3 cut(s) 450, 1151, 3039
Zsp2I ATGCAT 3 cut(s) 1708, 2700, 2878
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.