pycom04g20870
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0011826
Physical Location & Seq
Forward (+)
22296927 .. 22300372
3446 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g20870.1

Sequence Viewer

Length: 3315 bp
ATGGTGAAAATGTCATATGGTTTGTTGATTACTATAACTGTTCTTGTTGTTGCCCTTATGGTTTTCGAATCCGAGGGGATCAACGATGAGGGTCGATTTCTGCTGGAAGTAAAGAGCAAGTTAGTTGATAGATCTGATCATCTTGGTAGTTGGAATTCCAATGATTTCACACCTTGTGGATGGAGAGGTGTGAATTGCAGCAACGGATACAATCCGGTGGTGGTGTCTCTTGACTTGAACTCCATGAATCTTTCAGGTTATTTGTCTCCGAGCATTGGTGGATTGGTTCACCTGAACTATCTTAATCTTTCTTTCAATGAACTGACTCGGATTTTACCTAAAGACATCGGAAATTGTTCGAGTTTGGAGGTTCTAGCTTTGAATAACAATAAGTTTATAGGTCAAATTCCGAAAGCAATAGCTAGACTTTCAAGTTTACGAGTACTGAATGTCTGCAACAATAGATTATCAGGGCCTCTCCATGAAGAAATTGGAAATCTTTCATCCCTGTCCCAGCTGATTGCGTACTCTAACAATCTTAGCGGAATTCTGCCGCTCTCTCTTGGAAATCTCAAAAGATTGACGACTTTCAGGGTGGGGGAGAACTTGATATCCGGAAGCTTGCCTGCAGAGATAGGTGGATGTGTGAGCTTGGAGTATCTTGGTCTAGCGCAAAATCAGTTGACCGGAGAAATGCCTAAGGAGCTCGGGATGCTTGAGAATCTGTTAACTTTAATTCTTTGGGGTAACAAGCTTTCCGGGGTTATTCCAAAGGAGCTTGGCAATTGTACAAATTTGGAAATTCTTGCCCTGTATGAGAACAAGCTTGTGGGAGAGATTCCAAAGGAGCTTGGAAACATTGTGTATCTGGAGAAGTTGTATATTTACAGAAATATGTTGAATGGGACAGTTCCAAGGGAGATTGGCAATCTTGCTCTAGCAACAGAGATTGATCGATCAGAGAACTTATTGTCGGGAGAGATACCAGTTGAGCTCAGTAAGATAGCGGGATTGCAATTGCTTTATCTTTTCCAGAACCAATTCACAGGTGTTATTCCAGATGAGTTCACAAGTCTTACAAACCTGACGAAGCTCGACCTCTCTATTAATTTTTTAACAGGTTCAATTCCTACCGGATTCCAGCACTTGACTGAACTGATCATGCTGCAATTGTTCAACAATTCGCTGAGTGGCGTAATCCCTCAAGGCCTTGGAGTGTATAGCCCGCTTTGGGTGGTTGACTTGTCTGAAAACTTCCTGACAGGGGAAGTTCCTCGTCATCTCTGCAGAAATTCTAACATGATATTGTTAAATTTGGGGTCTAACAGGCTCACTGGGAATATACCAAACGGCATCACAAGCTGCAAGTCATTGGTGCAGCTTCGTCTAGTTGGAAACAACCTCACGGGGACTTTTCCATCCGAAATGTGCAAACTGGTTAACCTTTCTACAGTGGTGTTGAATCAGAACAAGTTCAGTGGCGCCATTCCTCCGGAAATTGGTAACTGCAGAACTTTGCAAACGCTGCACCTTTCGGACAACTACTTCACATCAGAGTTGCCTAGAGAGATTGGTAGCCTCCCGCAGTTGGTGATCGATAATGTCTCATCAAATTCTCTTTCTGGAAGGATACCACCCGAATTTTTCAATTGTAAAACACTTCAACGGCTTGATCTTAGCAATAACAACTTTTCAGATGCATTGCCAAGTGAGATAGGAACACTTTCTCAGTTGGAGCTTCTCAAGCTTTCTGAAAACAATCTTTCTGGGAACATACCAGTTGCGCTGGGAAACCTCGTGCGTTTGACTGAGTTACAAATGGGTGGCAACTCATTTTCTGGTGGCATCCCAGCTGAGTTGGGTGCCCTTTCGAGCCTGCAGATTGCGTTAAATCTCAGTTTTAACAACCTCTCTGGCGAGATACCTCTTCAACTTGGGAATCTTATCTTACTCGAATTCCTTCTACTCAATAACAACAATTTGACCGGTGAAATTCCAGGTTCTTTCGAGAATCTTTTGAGTCTACTTGGCTGCAATTTGTCGTACAATGCCTTGACAGGGCCTATTCCTCAGTTACGGCTCTTTCAGAATACAACTGTTGACAGCTTTGTTGGGAATAAAGGACTCTGTGGCGGTCCTCTTGGTGAATGCAAAACACCAACATCTTCACTCTCTTTTACTCCGGATATGGTGAAGATAAACCCCCGCTTGGGTAAAATCATTGCTATCATTGCAGCAGCTATTGGTGGTGCTTTTCTCATTTTGATTGCAATCCTTGTATATGTCAAGAGACGTCCCACAAGCGTAGTTGCTTCATTGCAAGAAAAATCATTCACTTCGCCGGTTTTGGATATGTACTTTTGCCCAAAGGCTGCAGGGTTCACTTTCCAAGACTTGGTTGCGGCAACTGAAAATTTTGATGAGAGCTATGCAATTGGAAGAGGAGCTTGTGGGACCGTGTACAAAGCAGTCTTACCGAGGGGTCATATAGTTGCTGTTAAGAAGGTAGTATCCAACAGGGAGGGGAGCAATGTGGATAACAGTTTCCATGCTGAAATTTTAACTTTAGGAAAAATCAGGCATCGAAATATTGTAAAGCTATACGGCTTCTGCTACCACCAGGGTTCCAATCTTCTGCTTTACGAGTACATGGCAAGAGGCAGCTTGGGAGAATTGCTTCATGGAACATCTTGTAGTCTTGATTGGATAACGAGGTTCATGATTGCTCTTGGGGCTGCTCAGGGTCTCTCTTACTTGCATCATGACTGCAAACCGAAGATATTTCACCGTGATATAAAGTCCAATAACATTCTTCTTGATGATAAGTTTGAAGCTCGTGTTGGGGATTTCGGGTTGGCAAAGGTGATCGACATGCCACACTCTGAGACTATGTCTGCAGTTGCAGGTTCTTATGGATATATTGCCCCTGAGTATGCATACACTTTGAAGGTCACAGAAAAATGTGACATCTACAGCTACGGAGTAGTCCTTCTGGAGCTGCTGACTGGAAGAACGCCCGTACAGTCGATAGAGGAAGGTGGTGACCTGGTAACGTGGGTAAGAAACTATTTTCTGCACCATTCTTTGTCTTCAGGATTACTCGATGCTCGATTAGATTTACAAGATGAAGGTACTGTCTCTCACATGATTAATGTCTTGAAAATTGCTTTGATGTGCACAAGTACGTCCCCTTTCGACCGCCCCACCATGAGAGAAGTCGTTTCCATGCTTATTGGGTCAAATGAGTGGGAAGGGCACCTTGAAAGTGATTCATCAAATCATGATACCAATTCCATGGATAATACTGAACCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

1105

Amino Acids

120.46

Weight (kDa)

5.52

Isoelectric Point (pI)

32.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 28 - 67 2e-10 Leucine rich repeat N-terminal domain
LRR_14 PF23598 77 - 199 2.7e-13 Leucine-rich repeat region
LRR_14 PF23598 211 - 311 5.2e-08 Leucine-rich repeat region
LRR_8 PF13855 337 - 396 3.5e-07 Leucine rich repeat
LRR_14 PF23598 543 - 628 1e-07 Leucine-rich repeat region
LRR_8 PF13855 553 - 611 4.6e-07 Leucine rich repeat
Pkinase PF00069 809 - 1075 2.2e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 811 - 1076 1.6e-41 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011826)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G63930 AT5G63930
fragaria_vesca FvH4_6g01320 FvH4_6g01320
malus_domestica MD04G1235000.v1.1 MD12G1253000.v1.1
prunus_persica Prupe.6G354900_v2.0.a1 Prupe.6G354900_v2.0.a1
pyrus_communis pycom04g20870 pycom12g23210
rosa_chinensis RchiOBHm_Chr3g0448891
rosa_laevigata RLG00000025869
rosa_multiflora Rmu_co8340729.1_g000001 Rmu_sc0003116.1_g000020
rosa_rugosa Rorug02G0617000
rosa_samantha Rh3AG015200 Rh3CG013900 Rh3DG015500
rosa_wichuraiana Rw3G001130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2296
Acc36I ACCTGC 1 cut(s) 2894
AccB1I GGYRCC 3 cut(s) 1481, 1862, 3255
AccB7I CCANNNNNTGG 1 cut(s) 2395
AccBSI CCGCTC 1 cut(s) 556
AccI GTMKAC 1 cut(s) 2023
AccIII TCCGGA 3 cut(s) 614, 1492, 2182
AciI CCGC 9 cut(s) 543, 554, 1007, 1226, 1583, 2133, 2206, 2402, 3199
AclWI GGATC 1 cut(s) 86
AcuI CTGAAG 1 cut(s) 3075
AcyI GRCGYC 2 cut(s) 1482, 2293
AdeI CACNNNGTG 1 cut(s) 176
AfiI CCNNNNNNNGG 9 cut(s) 275, 1231, 1264, 1499, 1588, 2058, 2209, 2210, 2395
AgeI ACCGGT 1 cut(s) 1985
AjnI CCWGG 3 cut(s) 1996, 2619, 3045
AjuI GAANNNNNNNTTGG 2 cut(s) 2823, 2855
AloI GAACNNNNNNTCC 2 cut(s) 596, 628
Alw21I GWGCWC 3 cut(s) 708, 996, 3179
Alw26I GTCTC 7 cut(s) 231, 270, 1609, 2284, 2750, 2879, 3142
Alw44I GTGCAC 1 cut(s) 3175
AlwI GGATC 1 cut(s) 86
Ama87I CYCGRG 1 cut(s) 707
Aor13HI TCCGGA 3 cut(s) 614, 1492, 2182
AoxI GGCC 3 cut(s) 473, 1207, 2060
ApaLI GTGCAC 1 cut(s) 3175
AseI ATTAAT 2 cut(s) 1107, 3150
AsiGI ACCGGT 1 cut(s) 1985
Asp700I GAANNNNTTC 7 cut(s) 355, 499, 1040, 1472, 1723, 1959, 2676
AspLEI GCGC 3 cut(s) 673, 1484, 1786
AspS9I GGNCC 4 cut(s) 473, 2060, 2135, 2454
AsuC2I CCSGG 1 cut(s) 760
AsuHPI GGTGA 9 cut(s) 16, 281, 1603, 2000, 2156, 2203, 2777, 2875, 3053
AsuII TTCGAA 1 cut(s) 66
AvaI CYCGRG 1 cut(s) 707
AvaII GGWCC 2 cut(s) 2135, 2454
AxyI CCTNAGG 1 cut(s) 699
BaeGI GKGCMC 3 cut(s) 1867, 3179, 3258
BanI GGYRCC 3 cut(s) 1481, 1862, 3255
BanII GRGCYC 2 cut(s) 708, 996
BauI CACGAG 2 cut(s) 1796, 2835
BbsI GAAGAC 1 cut(s) 3081
Bbv12I GWGCWC 3 cut(s) 708, 996, 3179
BccI CCATC 2 cut(s) 174, 1426
BceAI ACGGC 4 cut(s) 1366, 1682, 2093, 2620
BciT130I CCWGG 3 cut(s) 1998, 2621, 3047
BciVI GTATCC 3 cut(s) 200, 1623, 2521
BclI TGATCA 2 cut(s) 136, 1158
BcnI CCSGG 1 cut(s) 760
BcoDI GTCTC 7 cut(s) 231, 270, 1609, 2284, 2750, 2879, 3142
BfaI CTAG 6 cut(s) 374, 423, 668, 938, 1388, 1563
BfmI CTRYAG 8 cut(s) 627, 1285, 1449, 1507, 1877, 2373, 2895, 2971
BfoI RGCGCY 1 cut(s) 1485
BfuAI ACCTGC 1 cut(s) 2894
BfuI GTATCC 3 cut(s) 200, 1623, 2521
BglII AGATCT 1 cut(s) 131
BmcAI AGTACT 1 cut(s) 444
Bme1390I CCNGG 4 cut(s) 760, 1998, 2621, 3047
Bme18I GGWCC 2 cut(s) 2135, 2454
BmeT110I CYCGRG 1 cut(s) 707
BmgT120I GGNCC 4 cut(s) 473, 2060, 2135, 2454
BmiI GGNNCC 5 cut(s) 1483, 1864, 2455, 2626, 3257
BmrFI CCNGG 4 cut(s) 760, 1998, 2621, 3047
BmrI ACTGGG 1 cut(s) 1344
BmsI GCATC 7 cut(s) 702, 1362, 1687, 1854, 2590, 2767, 3094
BmuI ACTGGG 1 cut(s) 1344
BpiI GAAGAC 1 cut(s) 3081
BpmI CTGGAG 2 cut(s) 890, 3014
Bpu10I CCTNAGC 1 cut(s) 2739
Bpu14I TTCGAA 1 cut(s) 66
BpuEI CTTGAG 3 cut(s) 737, 1188, 1727
BpuMI CCSGG 1 cut(s) 760
Bsa29I ATCGAT 2 cut(s) 955, 1596
BsaBI GATNNNNATC 1 cut(s) 2270
BsaHI GRCGYC 2 cut(s) 1482, 2293
BsaI GGTCTC 1 cut(s) 2750
BsaJI CCNNGG 7 cut(s) 72, 759, 914, 1210, 2477, 2620, 3294
BsaWI WCCGGW 7 cut(s) 214, 614, 686, 1133, 1492, 1985, 2182
BsaXI ACNNNNNCTCC 4 cut(s) 224, 254, 863, 893
Bsc4I CCNNNNNNNGG 9 cut(s) 275, 1231, 1264, 1499, 1588, 2058, 2209, 2210, 2395
Bse118I RCCGGY 2 cut(s) 1985, 2341
Bse1I ACTGG 5 cut(s) 986, 1339, 1440, 1778, 3010
Bse21I CCTNAGG 1 cut(s) 699
Bse3DI GCAATG 5 cut(s) 1700, 2220, 2229, 2315, 2536
Bse8I GATNNNNATC 1 cut(s) 2270
BseAI TCCGGA 3 cut(s) 614, 1492, 2182
BseBI CCWGG 3 cut(s) 1998, 2621, 3047
BseCI ATCGAT 2 cut(s) 955, 1596
BseDI CCNNGG 7 cut(s) 72, 759, 914, 1210, 2477, 2620, 3294
BseGI GGATG 6 cut(s) 185, 503, 647, 717, 1418, 1845
BseJI GATNNNNATC 1 cut(s) 2270
BseLI CCNNNNNNNGG 9 cut(s) 275, 1231, 1264, 1499, 1588, 2058, 2209, 2210, 2395
BseMI GCAATG 5 cut(s) 1700, 2220, 2229, 2315, 2536
BseNI ACTGG 5 cut(s) 986, 1339, 1440, 1778, 3010
BseRI GAGGAG 1 cut(s) 2457
BseSI GKGCMC 3 cut(s) 1867, 3179, 3258
BseYI CCCAGC 3 cut(s) 513, 1786, 1849
BsgI GTGCAG 3 cut(s) 1397, 1511, 3059
Bsh1285I CGRYCG 1 cut(s) 3199
BshFI GGCC 3 cut(s) 475, 1209, 2062
BshNI GGYRCC 3 cut(s) 1481, 1862, 3255
BshTI ACCGGT 1 cut(s) 1985
BshVI ATCGAT 2 cut(s) 955, 1596
BsiEI CGRYCG 1 cut(s) 3199
BsiHKAI GWGCWC 3 cut(s) 708, 996, 3179
BsiHKCI CYCGRG 1 cut(s) 707
BsiSI CCGG 9 cut(s) 215, 615, 687, 759, 1134, 1493, 1986, 2183, 2342
BslFI GGGAC 6 cut(s) 496, 919, 1423, 2280, 2467, 3172
BslI CCNNNNNNNGG 9 cut(s) 275, 1231, 1264, 1499, 1588, 2058, 2209, 2210, 2395
BsmAI GTCTC 7 cut(s) 231, 270, 1609, 2284, 2750, 2879, 3142
BsmBI CGTCTC 1 cut(s) 2284
BsmFI GGGAC 6 cut(s) 496, 919, 1423, 2280, 2467, 3172
BsmI GAATGC 1 cut(s) 2153
BsnI GGCC 3 cut(s) 475, 1209, 2062
Bso31I GGTCTC 1 cut(s) 2750
BsoBI CYCGRG 1 cut(s) 707
Bsp119I TTCGAA 1 cut(s) 66
Bsp1286I GDGCHC 5 cut(s) 708, 996, 1867, 3179, 3258
Bsp13I TCCGGA 3 cut(s) 614, 1492, 2182
Bsp1407I TGTACA 2 cut(s) 788, 2460
Bsp143I GATC 9 cut(s) 78, 131, 136, 952, 956, 1158, 1593, 1672, 2865
Bsp19I CCATGG 1 cut(s) 3294
BspACI CCGC 9 cut(s) 543, 554, 1007, 1226, 1583, 2133, 2206, 2402, 3199
BspANI GGCC 3 cut(s) 475, 1209, 2062
BspDI ATCGAT 2 cut(s) 955, 1596
BspEI TCCGGA 3 cut(s) 614, 1492, 2182
BspHI TCATGA 3 cut(s) 2718, 2761, 3280
BspLI GGNNCC 5 cut(s) 1483, 1864, 2455, 2626, 3257
BspMAI CTGCAG 6 cut(s) 631, 1289, 1511, 1881, 2377, 2899
BspMI ACCTGC 1 cut(s) 2894
BspPI GGATC 1 cut(s) 86
BspT104I TTCGAA 1 cut(s) 66
BspT107I GGYRCC 3 cut(s) 1481, 1862, 3255
BspTNI GGTCTC 1 cut(s) 2750
BsrBI CCGCTC 1 cut(s) 556
BsrDI GCAATG 5 cut(s) 1700, 2220, 2229, 2315, 2536
BsrFI RCCGGY 2 cut(s) 1985, 2341
BsrGI TGTACA 2 cut(s) 788, 2460
BsrI ACTGG 5 cut(s) 986, 1339, 1440, 1778, 3010
BssAI RCCGGY 2 cut(s) 1985, 2341
BssECI CCNNGG 7 cut(s) 72, 759, 914, 1210, 2477, 2620, 3294
BssMI GATC 9 cut(s) 78, 131, 136, 952, 956, 1158, 1593, 1672, 2865
BssNI GRCGYC 2 cut(s) 1482, 2293
BssSI CACGAG 2 cut(s) 1796, 2835
BssT1I CCWWGG 3 cut(s) 914, 1210, 3294
Bst2BI CACGAG 2 cut(s) 1796, 2835
Bst2UI CCWGG 3 cut(s) 1998, 2621, 3047
Bst4CI ACNGT 9 cut(s) 40, 910, 1453, 2098, 2458, 2543, 2789, 3024, 3136
Bst6I CTCTTC 2 cut(s) 1932, 2434
BstACI GRCGYC 2 cut(s) 1482, 2293
BstAPI GCANNNNNTGC 1 cut(s) 1525
BstAUI TGTACA 2 cut(s) 788, 2460
BstBI TTCGAA 1 cut(s) 66
BstC8I GCNNGC 4 cut(s) 623, 627, 1226, 1877
BstDSI CCRYGG 1 cut(s) 3294
BstEII GGTNACC 1 cut(s) 3041
BstF5I GGATG 6 cut(s) 185, 503, 647, 717, 1418, 1845
BstH2I RGCGCY 1 cut(s) 1485
BstHHI GCGC 3 cut(s) 673, 1484, 1786
BstKTI GATC 9 cut(s) 81, 134, 139, 955, 959, 1161, 1596, 1675, 2868
BstMAI GTCTC 7 cut(s) 231, 270, 1609, 2284, 2750, 2879, 3142
BstMBI GATC 9 cut(s) 78, 131, 136, 952, 956, 1158, 1593, 1672, 2865
BstMCI CGRYCG 1 cut(s) 3199
BstMWI GCNNNNNNNGC 7 cut(s) 703, 712, 1359, 1525, 1744, 2231, 2732
BstNI CCWGG 3 cut(s) 1998, 2621, 3047
BstNSI RCATGY 1 cut(s) 2875
BstPI GGTNACC 1 cut(s) 3041
BstSCI CCNGG 4 cut(s) 758, 1996, 2619, 3045
BstSFI CTRYAG 8 cut(s) 627, 1285, 1449, 1507, 1877, 2373, 2895, 2971
BstSLI GKGCMC 3 cut(s) 1867, 3179, 3258
BstV2I GAAGAC 1 cut(s) 3081
BstX2I RGATCY 1 cut(s) 131
BstXI CCANNNNNNTGG 1 cut(s) 3295
BstYI RGATCY 1 cut(s) 131
Bsu15I ATCGAT 2 cut(s) 955, 1596
Bsu36I CCTNAGG 1 cut(s) 699
BsuI GTATCC 3 cut(s) 200, 1623, 2521
BsuRI GGCC 3 cut(s) 475, 1209, 2062
BsuTUI ATCGAT 2 cut(s) 955, 1596
BtgI CCRYGG 1 cut(s) 3294
BtsCI GGATG 6 cut(s) 185, 503, 647, 717, 1418, 1845
BtsIMutI CAGTG 3 cut(s) 1332, 1458, 1483
BveI ACCTGC 1 cut(s) 2894
Cac8I GCNNGC 4 cut(s) 623, 627, 1226, 1877
CciI TCATGA 3 cut(s) 2718, 2761, 3280
CfoI GCGC 3 cut(s) 673, 1484, 1786
Cfr10I RCCGGY 2 cut(s) 1985, 2341
Cfr13I GGNCC 4 cut(s) 473, 2060, 2135, 2454
ClaI ATCGAT 2 cut(s) 955, 1596
CsiI ACCWGGT 1 cut(s) 3045
CspAI ACCGGT 1 cut(s) 1985
CspCI CAANNNNNGTGG 2 cut(s) 1459, 1494
DinI GGCGCC 1 cut(s) 1483
DpnI GATC 9 cut(s) 80, 133, 138, 954, 958, 1160, 1595, 1674, 2867
DpnII GATC 9 cut(s) 78, 131, 136, 952, 956, 1158, 1593, 1672, 2865
DraIII CACNNNGTG 1 cut(s) 176
Eam1104I CTCTTC 2 cut(s) 1932, 2434
EarI CTCTTC 2 cut(s) 1932, 2434
Ecl136II GAGCTC 2 cut(s) 706, 994
Eco130I CCWWGG 3 cut(s) 914, 1210, 3294
Eco147I AGGCCT 1 cut(s) 1209
Eco24I GRGCYC 2 cut(s) 708, 996
Eco31I GGTCTC 1 cut(s) 2750
Eco32I GATATC 1 cut(s) 612
Eco47I GGWCC 2 cut(s) 2135, 2454
Eco53kI GAGCTC 2 cut(s) 706, 994
Eco57I CTGAAG 1 cut(s) 3075
Eco81I CCTNAGG 1 cut(s) 699
Eco88I CYCGRG 1 cut(s) 707
Eco91I GGTNACC 1 cut(s) 3041
EcoICRI GAGCTC 2 cut(s) 706, 994
EcoO109I RGGNCCY 2 cut(s) 473, 2060
EcoO65I GGTNACC 1 cut(s) 3041
EcoRI GAATTC 3 cut(s) 154, 546, 1955
EcoRII CCWGG 3 cut(s) 1996, 2619, 3045
EcoRV GATATC 1 cut(s) 612
EcoT14I CCWWGG 3 cut(s) 914, 1210, 3294
EcoT22I ATGCAT 2 cut(s) 1702, 2938
EcoT38I GRGCYC 2 cut(s) 708, 996
EgeI GGCGCC 1 cut(s) 1483
EheI GGCGCC 1 cut(s) 1483
ErhI CCWWGG 3 cut(s) 914, 1210, 3294
Esp3I CGTCTC 1 cut(s) 2284
FalI AAGNNNNNCTT 4 cut(s) 2431, 2463, 3243, 3275
FaqI GGGAC 6 cut(s) 496, 919, 1423, 2280, 2467, 3172
FauI CCCGC 4 cut(s) 1000, 1233, 1590, 2213
FauNDI CATATG 1 cut(s) 16
FbaI TGATCA 2 cut(s) 136, 1158
FblI GTMKAC 1 cut(s) 2023
FokI GGATG 6 cut(s) 192, 490, 654, 724, 1405, 1832
FriOI GRGCYC 2 cut(s) 708, 996
FspBI CTAG 6 cut(s) 374, 423, 668, 938, 1388, 1563
GlaI GCGC 3 cut(s) 672, 1483, 1785
GsaI CCCAGC 3 cut(s) 517, 1790, 1853
GsuI CTGGAG 2 cut(s) 890, 3014
HaeII RGCGCY 1 cut(s) 1485
HaeIII GGCC 3 cut(s) 475, 1209, 2062
HapII CCGG 9 cut(s) 215, 615, 687, 759, 1134, 1493, 1986, 2183, 2342
HhaI GCGC 3 cut(s) 673, 1484, 1786
Hin1I GRCGYC 2 cut(s) 1482, 2293
Hin6I GCGC 3 cut(s) 671, 1482, 1784
HinP1I GCGC 3 cut(s) 671, 1482, 1784
HincII GTYRAC 5 cut(s) 684, 729, 1240, 1441, 2101
HindII GTYRAC 5 cut(s) 684, 729, 1240, 1441, 2101
HindIII AAGCTT 4 cut(s) 619, 752, 824, 1745
HpaI GTTAAC 2 cut(s) 729, 1441
HpaII CCGG 9 cut(s) 215, 615, 687, 759, 1134, 1493, 1986, 2183, 2342
HphI GGTGA 9 cut(s) 16, 281, 1603, 2000, 2156, 2203, 2777, 2875, 3053
HpyAV CCTTC 8 cut(s) 1620, 1970, 2497, 2941, 2999, 3029, 3122, 3245
HpyCH4III ACNGT 9 cut(s) 40, 910, 1453, 2098, 2458, 2543, 2789, 3024, 3136
HpyCH4IV ACGT 3 cut(s) 2293, 3053, 3185
HpyF10VI GCNNNNNNNGC 7 cut(s) 703, 712, 1359, 1525, 1744, 2231, 2732
HpySE526I ACGT 3 cut(s) 2293, 3053, 3185
Hsp92I GRCGYC 2 cut(s) 1482, 2293
HspAI GCGC 3 cut(s) 671, 1482, 1784
KasI GGCGCC 1 cut(s) 1481
Kpn2I TCCGGA 3 cut(s) 614, 1492, 2182
Ksp22I TGATCA 2 cut(s) 136, 1158
KspAI GTTAAC 2 cut(s) 729, 1441
Kzo9I GATC 9 cut(s) 78, 131, 136, 952, 956, 1158, 1593, 1672, 2865
LmnI GCTCC 7 cut(s) 703, 775, 847, 1735, 2444, 2526, 2995
LweI GCATC 7 cut(s) 702, 1362, 1687, 1854, 2590, 2767, 3094
MabI ACCWGGT 1 cut(s) 3045
MaeI CTAG 6 cut(s) 374, 423, 668, 938, 1388, 1563
MaeII ACGT 3 cut(s) 2293, 3053, 3185
MaeIII GTNAC 8 cut(s) 746, 1502, 1812, 2073, 2950, 2963, 3041, 3049
MalI GATC 9 cut(s) 80, 133, 138, 954, 958, 1160, 1595, 1674, 2867
MbiI CCGCTC 1 cut(s) 556
MboI GATC 9 cut(s) 78, 131, 136, 952, 956, 1158, 1593, 1672, 2865
MfeI CAATTG 5 cut(s) 784, 1016, 1169, 1648, 2433
MflI RGATCY 1 cut(s) 131
MhlI GDGCHC 5 cut(s) 708, 996, 1867, 3179, 3258
Mly113I GGCGCC 1 cut(s) 1482
MlyI GAGTC 3 cut(s) 319, 2029, 2118
MmeI TCCRAC 4 cut(s) 131, 1372, 1713, 2538
Mph1103I ATGCAT 2 cut(s) 1702, 2938
MroI TCCGGA 3 cut(s) 614, 1492, 2182
MroXI GAANNNNTTC 7 cut(s) 355, 499, 1040, 1472, 1723, 1959, 2676
MspA1I CMGCKG 2 cut(s) 517, 1853
MspI CCGG 9 cut(s) 215, 615, 687, 759, 1134, 1493, 1986, 2183, 2342
MspR9I CCNGG 4 cut(s) 760, 1998, 2621, 3047
MunI CAATTG 5 cut(s) 784, 1016, 1169, 1648, 2433
Mva1269I GAATGC 1 cut(s) 2153
MvaI CCWGG 3 cut(s) 1998, 2621, 3047
MwoI GCNNNNNNNGC 7 cut(s) 703, 712, 1359, 1525, 1744, 2231, 2732
NarI GGCGCC 1 cut(s) 1482
NciI CCSGG 1 cut(s) 760
NcoI CCATGG 1 cut(s) 3294
NdeI CATATG 1 cut(s) 16
NdeII GATC 9 cut(s) 78, 131, 136, 952, 956, 1158, 1593, 1672, 2865
NlaIV GGNNCC 5 cut(s) 1483, 1864, 2455, 2626, 3257
NmuCI GTSAC 3 cut(s) 2950, 2963, 3041
NsiI ATGCAT 2 cut(s) 1702, 2938
NspI RCATGY 1 cut(s) 2875
NspV TTCGAA 1 cut(s) 66
PagI TCATGA 3 cut(s) 2718, 2761, 3280
PceI AGGCCT 1 cut(s) 1209
PctI GAATGC 1 cut(s) 2153
PdmI GAANNNNTTC 7 cut(s) 355, 499, 1040, 1472, 1723, 1959, 2676
PfeI GAWTC 9 cut(s) 68, 247, 721, 838, 1137, 1462, 1939, 2011, 3269
PflFI GACNNNGTC 1 cut(s) 2890
PflMI CCANNNNNTGG 1 cut(s) 2395
PinAI ACCGGT 1 cut(s) 1985
PleI GAGTC 3 cut(s) 319, 2028, 2118
PluTI GGCGCC 1 cut(s) 1485
PpsI GAGTC 3 cut(s) 319, 2028, 2118
PshBI ATTAAT 2 cut(s) 1107, 3150
Psp124BI GAGCTC 2 cut(s) 708, 996
Psp6I CCWGG 3 cut(s) 1996, 2619, 3045
PspEI GGTNACC 1 cut(s) 3041
PspFI CCCAGC 3 cut(s) 513, 1786, 1849
PspGI CCWGG 3 cut(s) 1996, 2619, 3045
PspN4I GGNNCC 5 cut(s) 1483, 1864, 2455, 2626, 3257
PspPI GGNCC 4 cut(s) 473, 2060, 2135, 2454
PstI CTGCAG 6 cut(s) 631, 1289, 1511, 1881, 2377, 2899
PsuI RGATCY 1 cut(s) 131
PsyI GACNNNGTC 1 cut(s) 2890
PvuII CAGCTG 2 cut(s) 517, 1853
SacI GAGCTC 2 cut(s) 708, 996
Sau3AI GATC 9 cut(s) 78, 131, 136, 952, 956, 1158, 1593, 1672, 2865
Sau96I GGNCC 4 cut(s) 473, 2060, 2135, 2454
ScaI AGTACT 1 cut(s) 444
SchI GAGTC 3 cut(s) 319, 2029, 2118
ScrFI CCNGG 4 cut(s) 760, 1998, 2621, 3047
SduI GDGCHC 5 cut(s) 708, 996, 1867, 3179, 3258
SexAI ACCWGGT 1 cut(s) 3045
SfaNI GCATC 7 cut(s) 702, 1362, 1687, 1854, 2590, 2767, 3094
SfcI CTRYAG 8 cut(s) 627, 1285, 1449, 1507, 1877, 2373, 2895, 2971
SfoI GGCGCC 1 cut(s) 1483
SfuI TTCGAA 1 cut(s) 66
SinI GGWCC 2 cut(s) 2135, 2454
SmlI CTYRAG 3 cut(s) 716, 1203, 1742
SmoI CTYRAG 3 cut(s) 716, 1203, 1742
SseBI AGGCCT 1 cut(s) 1209
SsiI CCGC 9 cut(s) 543, 554, 1007, 1226, 1583, 2133, 2206, 2402, 3199
SspDI GGCGCC 1 cut(s) 1481
SspI AATATT 1 cut(s) 2590
SspMI CTAG 6 cut(s) 374, 423, 668, 938, 1388, 1563
SstI GAGCTC 2 cut(s) 708, 996
StuI AGGCCT 1 cut(s) 1209
StyD4I CCNGG 4 cut(s) 758, 1996, 2619, 3045
StyI CCWWGG 3 cut(s) 914, 1210, 3294
TaaI ACNGT 9 cut(s) 40, 910, 1453, 2098, 2458, 2543, 2789, 3024, 3136
TaiI ACGT 3 cut(s) 2296, 3056, 3188
TatI WGTACW 5 cut(s) 442, 788, 2355, 2460, 2646
TauI GCSGC 2 cut(s) 556, 2405
TfiI GAWTC 9 cut(s) 68, 247, 721, 838, 1137, 1462, 1939, 2011, 3269
TscAI CASTG 3 cut(s) 1339, 1458, 1483
TseFI GTSAC 3 cut(s) 2950, 2963, 3041
Tsp45I GTSAC 3 cut(s) 2950, 2963, 3041
TspDTI ATGAA 9 cut(s) 260, 333, 492, 498, 2304, 2669, 2707, 3141, 3261
TspGWI ACGGA 2 cut(s) 219, 2994
TspRI CASTG 3 cut(s) 1339, 1458, 1483
Tth111I GACNNNGTC 1 cut(s) 2890
Van91I CCANNNNNTGG 1 cut(s) 2395
VneI GTGCAC 1 cut(s) 3175
VpaK11BI GGWCC 2 cut(s) 2135, 2454
VspI ATTAAT 2 cut(s) 1107, 3150
XceI RCATGY 1 cut(s) 2875
XcmI CCANNNNNNNNNTGG 1 cut(s) 488
XmiI GTMKAC 1 cut(s) 2023
XmnI GAANNNNTTC 7 cut(s) 355, 499, 1040, 1472, 1723, 1959, 2676
XspI CTAG 6 cut(s) 374, 423, 668, 938, 1388, 1563
ZraI GACGTC 1 cut(s) 2294
ZrmI AGTACT 1 cut(s) 444
Zsp2I ATGCAT 2 cut(s) 1702, 2938
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.