MD04G1235000.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0011826
Physical Location & Seq
Forward (+)
31412482 .. 31416441
3960 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1235000.v1.1.491

Sequence Viewer

Length: 3315 bp
ATGGTGAATATGTCATATGGTTTGTTGATTACTATAACTGTTCTTGTTGTTGCCCTTATGGTTTTCGAATCCGAGGGGATCAACGATGAGGGTCAATATCTTCTAGAAGTAAAGAGCAGATTAGTTGATCGGTTTGATCATCTTGGTAGCTGGAATCCCAATGATTTCACACCTTGTGGATGGAGAGGTGTGAATTGCAGCAACGGATACAATCCGGTGGTGGTGTCTCTTGACTTGAACTCCATGAATCTTTCAGGTTATTTGTCTCCGAGCATTGGTGGATTGGTTCACCTGAACTATCTTAATCTTTCTTTCAATGAACTGACTCGGACTTTACCTAAAGACATCGGAAATTGCTCGAGTTTGGAGGTTCTAGCTTTGAATAACAATAAGTTTATAGGTCAAATTCCGAAAGCAATAGCTAGACTTTCAAGTTTACGAGTACTGAATATCTGCAACAATAGATTATCAGGGCCTCTCCATGAAGAGATTGGAAATCTTTCATCCCTGTCCCAGCTGATTGCGTACTCTAACAATCTTAGCGGAATTCTGCCGCTCTCTCTTGGAAATCTCAAAAGATTGACGACTTTCAGGGCGGGGGAGAACTTGATATCCGGAAGCTTGCCTGCAGAGATAGGTGGATGTGCGAGCTTGCAGTATCTTGGTCTAGCGGAAAATCAGTTAACCGGAGAAATGCCTAAGGAGCTCGGGATGCTTGAGAAACTGAGAACATTAATTCTTTGGAGTAACCATCTTTCCGGGGTTATTCCAAAGGAGCTTGGCAATTGTACAAATTTGGAAATTCTTGCCCTGTATCAGAACAAGCTTGTGGGAGAGATTCCAAAGGAGCTTGGAAACATTGTGTATCTGGAGAAGTTGTATATTTACAGAAATATGTTGAATGGGACAGTTCCAAGAGAGATTGGCAATCTTTCTCTAGCAACAGAGATTGATTTTTCGGAGAACTTATTGTCAGGAGAGATCCCAGTTGAGCTCAGCAAGATAGAGGGATTGCAATTGCTTTATCTTTTCCAGAACCAATTCACAGGAGTTATTCCAGATGAGTTCACAAGTCTTACAAACCTGACTAAGCTCGACCTCTCTATTAATTTTTTAACAGGTTCAATTCCTACCGGATTCCAGTACTTGACTGAACTGATCATGCTGCAATTGTTCAACAATTCGCTGAGTGGCGTAATCCCTCAAGGCCTTGGAGTGTATAGCCGGCTTTGGGTGGTTGACTTATCCGAAAACTTCCTAACAGGGGAAGTTCCTCGTCATCTCTGCAGAAATTCTAACATGATATTGTTAAATTTGGGGTTTAACAGGCTCGCTGGGAATATACCAAATGGCATCACAAGCTGCAAGTCATTGGTGCAGCTTCGTCTAGTTGGAAACAACCTCACGGGGACTTTTCCATCCGAAATGTGCAAACTGGTTAACCTTTCTACAGTGGAGTTGAATCAGAACAAGTTCAGCGGCGCCATTCCTCCGGAAATTGGTAACTGCAGAACTTTGCAAACGCTGCACCTTTCGGACAACTACTTCACATCAGAGTTGCCTAGAGAGATTGGTAGCCTCCCACAGTTGGTGATCTTTAATGTCTCATCAAATTCTCTTTCTGGAAGGATACCGCCCGAAATTTTCAATTGTAAAACACTTCAACGGCTTGATCTTAGAAATAACAACTTTTCAGATGCATTGCCAAGTGAGATAGGAACACTTTCTCAATTGGAGCTTCTCAAGCTTTCTGAAAACAATCTTTCTGGGAACATACCAGTTGCGCTGGGAAACCTCGTGCGTTTGACTGAGTTACAAATGGGTGGCAACTCATTTTCTGGTAGCATCCCAGCTGAGTTGGGTGCCCTTTCGAGCCTGCAGATTGCGTTAAATCTCAGTTTTAACAACCTCTCTGGCGAGATACCTCCTCAACTTGGGAATCTTATCTTACTGGAATTCCTTCTACTCAATAACAACAATTTGACCGGTGAAATTCCAGGTTCTTTCGAGAATCTTTTGAGTCTACTTGGCTGCAACTTGTCGTACAATGCCTTGACGGGGCCTATTCCTCTGTTACGGCTCTTTCAGAATACGACTGTTGACAGCTTTGTTGGGAATAAAGAACTCTGTGGCGGTCCTCTTGGTGAATGCAAAACACCACCATCTTCACTCTCTTTTACTCCGGATATGGTGAAGAAAAACCCCCGCTTGGGTAAAATCATCGCTATCATTGCAGCAGCTATTGGTGGTGTTTCTCTCATGTTGATTGCAATCCTTGTATATGTCATGAGGCGTCCCACAAGCGTAGTTGCTTCATTGCAAGAAAAATCACTCTCTTCGCCGGTTCTGGATATGTACTTTTGCCCAAAGGCTGCAGGGTTCACTTTCCAAGACTTGGTTGCAGCGACTGAAAATTTTGATGAGAGCTATGCAATTGGAAGAGGAGCTTGTGGGACCGTGTACAAAGCAGTCTTACCAAGGGGTCATATAGTTGCTGTTAAGAAGGTAGTATCCAACAGGGAGGGGAGCAATGTGGATAACAGTTTCCATGCTGAAATTTTAACTTTAGGAAAAATCAGGCATCGAAATATTGTAAAGCTATACGGCTTCTGCTACCACCAGGGTTCCAATCTTCTGCTTTACGAGTACATGGCAAGAGGCAGCTTGGGAGAATTGCTTCATGGAACATCTTGTAGTCTTGATTGGATAACGAGGTTCATGATTGCTCTTGGGGCTGCTCAGGGCCTCTCTTACTTGCATCATGATTGCAAACCGAAGATCTTTCACCGTGATATAAAGTCCAATAACATTCTTCTTGATGATAAGTTTGAAGCTCGTGTTGGGGATTTCGGGTTGGCAAAAGTGATCGACATGCCACACTCTGAGACTATGTCTGCAGTTGCAGGTTCTTATGGATATATTGCCCCTGAGTATGCATACACTTTGAAAGTCACAGAAAAATGTGACATCTACAGCTACGGAGTAGTCCTTCTGGAGCTGCTGACTGGAAGAACGCCTGTACAGTCGATAGAGGAAGGCGGTGACCTGGTAACGTGGGTAAGAAACTATTTTCTGCACCATTCTTTGTCTTCAGGAGTACTCGATGCTCGATTAGATTTACAAGATGAAGGTACTGTCTCTCACATGATTAATGTCTTGAAAATTGCTTTGATGTGCACAAGTACGTCCCCTTTCGACCGCCCGACCATGAGACAAGTTGTTTCCATGCTTATTGGGTCAAATGAGTGGGAAGGACACTTTGAAAGTGATTCATCAAATCATGATACCAATTCCATGGATAATACTGAACCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

1105

Amino Acids

120.9

Weight (kDa)

5.52

Isoelectric Point (pI)

32.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 28 - 67 9.3e-10 Leucine rich repeat N-terminal domain
LRR_14 PF23598 77 - 198 2.7e-13 Leucine-rich repeat region
LRR_14 PF23598 211 - 311 6.3e-08 Leucine-rich repeat region
LRR_8 PF13855 337 - 396 2.3e-06 Leucine rich repeat
LRR_14 PF23598 475 - 537 3.5e-06 Leucine-rich repeat region
LRR_14 PF23598 543 - 628 2.2e-09 Leucine-rich repeat region
LRR_8 PF13855 553 - 611 2.2e-07 Leucine rich repeat
Pkinase PF00069 809 - 1075 1.6e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 811 - 1076 1.4e-41 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011826)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G63930 AT5G63930
fragaria_vesca FvH4_6g01320 FvH4_6g01320
malus_domestica MD04G1235000.v1.1 MD12G1253000.v1.1
prunus_persica Prupe.6G354900_v2.0.a1 Prupe.6G354900_v2.0.a1
pyrus_communis pycom04g20870 pycom12g23210
rosa_chinensis RchiOBHm_Chr3g0448891
rosa_laevigata RLG00000025869
rosa_multiflora Rmu_co8340729.1_g000001 Rmu_sc0003116.1_g000020
rosa_rugosa Rorug02G0617000
rosa_samantha Rh3AG015200 Rh3CG013900 Rh3DG015500
rosa_wichuraiana Rw3G001130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2894
AccB1I GGYRCC 2 cut(s) 1481, 1862
AccB7I CCANNNNNTGG 1 cut(s) 2395
AccBSI CCGCTC 1 cut(s) 556
AccI GTMKAC 1 cut(s) 2023
AccIII TCCGGA 3 cut(s) 614, 1492, 2182
AclWI GGATC 2 cut(s) 86, 976
AcuI CTGAAG 1 cut(s) 3075
AcyI GRCGYC 2 cut(s) 1482, 2293
AdeI CACNNNGTG 1 cut(s) 176
AgeI ACCGGT 1 cut(s) 1985
AjnI CCWGG 3 cut(s) 1996, 2619, 3045
AjuI GAANNNNNNNTTGG 2 cut(s) 2823, 2855
AloI GAACNNNNNNTCC 2 cut(s) 596, 628
Alw21I GWGCWC 3 cut(s) 708, 996, 3179
Alw26I GTCTC 6 cut(s) 231, 270, 1609, 2879, 3142, 3205
Alw44I GTGCAC 1 cut(s) 3175
AlwI GGATC 2 cut(s) 86, 976
AlwNI CAGNNNCTG 1 cut(s) 2408
Ama87I CYCGRG 2 cut(s) 358, 707
Aor13HI TCCGGA 3 cut(s) 614, 1492, 2182
AoxI GGCC 4 cut(s) 473, 1207, 2060, 2743
ApaLI GTGCAC 1 cut(s) 3175
AseI ATTAAT 3 cut(s) 734, 1107, 3150
AsiGI ACCGGT 1 cut(s) 1985
Asp700I GAANNNNTTC 6 cut(s) 499, 1040, 1472, 1723, 1959, 2676
AspLEI GCGC 2 cut(s) 1484, 1786
AspS9I GGNCC 5 cut(s) 473, 2060, 2135, 2454, 2743
AsuC2I CCSGG 1 cut(s) 760
AsuHPI GGTGA 8 cut(s) 16, 281, 1603, 2000, 2156, 2203, 2777, 3053
AsuII TTCGAA 1 cut(s) 66
AvaI CYCGRG 2 cut(s) 358, 707
AvaII GGWCC 2 cut(s) 2135, 2454
AxyI CCTNAGG 1 cut(s) 699
BaeGI GKGCMC 2 cut(s) 1867, 3179
BanI GGYRCC 2 cut(s) 1481, 1862
BanII GRGCYC 2 cut(s) 708, 996
BauI CACGAG 2 cut(s) 1796, 2835
BbsI GAAGAC 1 cut(s) 3081
Bbv12I GWGCWC 3 cut(s) 708, 996, 3179
BccI CCATC 4 cut(s) 174, 759, 1426, 2170
BceAI ACGGC 3 cut(s) 1682, 2093, 2620
BciT130I CCWGG 3 cut(s) 1998, 2621, 3047
BciVI GTATCC 3 cut(s) 200, 1623, 2521
BclI TGATCA 2 cut(s) 136, 1158
BcnI CCSGG 1 cut(s) 760
BcoDI GTCTC 6 cut(s) 231, 270, 1609, 2879, 3142, 3205
BfaI CTAG 7 cut(s) 104, 374, 423, 668, 938, 1388, 1563
BfmI CTRYAG 8 cut(s) 627, 1285, 1449, 1507, 1877, 2373, 2895, 2971
BfoI RGCGCY 1 cut(s) 1485
BfuAI ACCTGC 1 cut(s) 2894
BfuI GTATCC 3 cut(s) 200, 1623, 2521
BglII AGATCT 1 cut(s) 2778
BlpI GCTNAGC 1 cut(s) 995
BmcAI AGTACT 3 cut(s) 444, 1145, 3099
Bme1390I CCNGG 4 cut(s) 760, 1998, 2621, 3047
Bme18I GGWCC 2 cut(s) 2135, 2454
BmeT110I CYCGRG 2 cut(s) 358, 707
BmgT120I GGNCC 5 cut(s) 473, 2060, 2135, 2454, 2743
BmiI GGNNCC 5 cut(s) 1483, 1864, 2061, 2455, 2626
BmrFI CCNGG 4 cut(s) 760, 1998, 2621, 3047
BmrI ACTGGG 1 cut(s) 980
BmsI GCATC 7 cut(s) 702, 1362, 1687, 1854, 2590, 2767, 3094
BmuI ACTGGG 1 cut(s) 980
BpiI GAAGAC 1 cut(s) 3081
BpmI CTGGAG 2 cut(s) 890, 3014
Bpu10I CCTNAGC 1 cut(s) 2739
Bpu1102I GCTNAGC 1 cut(s) 995
Bpu14I TTCGAA 1 cut(s) 66
BpuEI CTTGAG 3 cut(s) 737, 1188, 1727
BpuMI CCSGG 1 cut(s) 760
BsaBI GATNNNNATC 1 cut(s) 2270
BsaHI GRCGYC 2 cut(s) 1482, 2293
BsaJI CCNNGG 6 cut(s) 72, 759, 1210, 2477, 2620, 3294
BsaWI WCCGGW 7 cut(s) 214, 614, 686, 1133, 1492, 1985, 2182
BsaXI ACNNNNNCTCC 4 cut(s) 224, 254, 863, 893
Bse118I RCCGGY 3 cut(s) 1224, 1985, 2341
Bse1I ACTGG 6 cut(s) 986, 1141, 1440, 1778, 1956, 3010
Bse21I CCTNAGG 1 cut(s) 699
Bse3DI GCAATG 4 cut(s) 1700, 2229, 2315, 2536
Bse8I GATNNNNATC 1 cut(s) 2270
BseAI TCCGGA 3 cut(s) 614, 1492, 2182
BseBI CCWGG 3 cut(s) 1998, 2621, 3047
BseDI CCNNGG 6 cut(s) 72, 759, 1210, 2477, 2620, 3294
BseGI GGATG 6 cut(s) 185, 503, 647, 717, 1418, 1845
BseJI GATNNNNATC 1 cut(s) 2270
BseMI GCAATG 4 cut(s) 1700, 2229, 2315, 2536
BseMII CTCAG 9 cut(s) 716, 1009, 1178, 1800, 1845, 1909, 2753, 2874, 2919
BseNI ACTGG 6 cut(s) 986, 1141, 1440, 1778, 1956, 3010
BseRI GAGGAG 2 cut(s) 1917, 2457
BseSI GKGCMC 2 cut(s) 1867, 3179
BseYI CCCAGC 4 cut(s) 513, 1334, 1786, 1849
BsgI GTGCAG 3 cut(s) 1397, 1511, 3059
Bsh1285I CGRYCG 1 cut(s) 3199
BshFI GGCC 4 cut(s) 475, 1209, 2062, 2745
BshNI GGYRCC 2 cut(s) 1481, 1862
BshTI ACCGGT 1 cut(s) 1985
BsiEI CGRYCG 1 cut(s) 3199
BsiHKAI GWGCWC 3 cut(s) 708, 996, 3179
BsiHKCI CYCGRG 2 cut(s) 358, 707
BslFI GGGAC 6 cut(s) 496, 919, 1423, 2280, 2467, 3172
BsmAI GTCTC 6 cut(s) 231, 270, 1609, 2879, 3142, 3205
BsmFI GGGAC 6 cut(s) 496, 919, 1423, 2280, 2467, 3172
BsmI GAATGC 1 cut(s) 2153
BsnI GGCC 4 cut(s) 475, 1209, 2062, 2745
BsoBI CYCGRG 2 cut(s) 358, 707
Bsp119I TTCGAA 1 cut(s) 66
Bsp1286I GDGCHC 4 cut(s) 708, 996, 1867, 3179
Bsp13I TCCGGA 3 cut(s) 614, 1492, 2182
Bsp1407I TGTACA 3 cut(s) 788, 2460, 3019
Bsp143I GATC 9 cut(s) 78, 127, 136, 981, 1158, 1593, 1672, 2778, 2865
Bsp1720I GCTNAGC 1 cut(s) 995
Bsp19I CCATGG 1 cut(s) 3294
BspANI GGCC 4 cut(s) 475, 1209, 2062, 2745
BspCNI CTCAG 9 cut(s) 717, 1008, 1179, 1801, 1846, 1908, 2752, 2875, 2920
BspEI TCCGGA 3 cut(s) 614, 1492, 2182
BspHI TCATGA 4 cut(s) 2286, 2718, 2761, 3280
BspLI GGNNCC 5 cut(s) 1483, 1864, 2061, 2455, 2626
BspMAI CTGCAG 6 cut(s) 631, 1289, 1511, 1881, 2377, 2899
BspMI ACCTGC 1 cut(s) 2894
BspPI GGATC 2 cut(s) 86, 976
BspT104I TTCGAA 1 cut(s) 66
BspT107I GGYRCC 2 cut(s) 1481, 1862
BsrBI CCGCTC 1 cut(s) 556
BsrDI GCAATG 4 cut(s) 1700, 2229, 2315, 2536
BsrFI RCCGGY 3 cut(s) 1224, 1985, 2341
BsrGI TGTACA 3 cut(s) 788, 2460, 3019
BsrI ACTGG 6 cut(s) 986, 1141, 1440, 1778, 1956, 3010
BssAI RCCGGY 3 cut(s) 1224, 1985, 2341
BssECI CCNNGG 6 cut(s) 72, 759, 1210, 2477, 2620, 3294
BssMI GATC 9 cut(s) 78, 127, 136, 981, 1158, 1593, 1672, 2778, 2865
BssNI GRCGYC 2 cut(s) 1482, 2293
BssSI CACGAG 2 cut(s) 1796, 2835
BssT1I CCWWGG 3 cut(s) 1210, 2477, 3294
Bst2BI CACGAG 2 cut(s) 1796, 2835
Bst2UI CCWGG 3 cut(s) 1998, 2621, 3047
Bst6I CTCTTC 3 cut(s) 480, 2341, 2434
BstACI GRCGYC 2 cut(s) 1482, 2293
BstAPI GCANNNNNTGC 1 cut(s) 1525
BstAUI TGTACA 3 cut(s) 788, 2460, 3019
BstBI TTCGAA 1 cut(s) 66
BstC8I GCNNGC 7 cut(s) 623, 627, 649, 653, 1226, 1332, 1877
BstDSI CCRYGG 1 cut(s) 3294
BstEII GGTNACC 1 cut(s) 3041
BstF5I GGATG 6 cut(s) 185, 503, 647, 717, 1418, 1845
BstH2I RGCGCY 1 cut(s) 1485
BstHHI GCGC 2 cut(s) 1484, 1786
BstKTI GATC 9 cut(s) 81, 130, 139, 984, 1161, 1596, 1675, 2781, 2868
BstMAI GTCTC 6 cut(s) 231, 270, 1609, 2879, 3142, 3205
BstMBI GATC 9 cut(s) 78, 127, 136, 981, 1158, 1593, 1672, 2778, 2865
BstMCI CGRYCG 1 cut(s) 3199
BstMWI GCNNNNNNNGC 7 cut(s) 703, 712, 1359, 1525, 1744, 2231, 2732
BstNI CCWGG 3 cut(s) 1998, 2621, 3047
BstNSI RCATGY 1 cut(s) 2875
BstPI GGTNACC 1 cut(s) 3041
BstSCI CCNGG 4 cut(s) 758, 1996, 2619, 3045
BstSFI CTRYAG 8 cut(s) 627, 1285, 1449, 1507, 1877, 2373, 2895, 2971
BstSLI GKGCMC 2 cut(s) 1867, 3179
BstV2I GAAGAC 1 cut(s) 3081
BstX2I RGATCY 2 cut(s) 981, 2778
BstXI CCANNNNNNTGG 1 cut(s) 3295
BstYI RGATCY 2 cut(s) 981, 2778
Bsu36I CCTNAGG 1 cut(s) 699
BsuI GTATCC 3 cut(s) 200, 1623, 2521
BsuRI GGCC 4 cut(s) 475, 1209, 2062, 2745
BtgI CCRYGG 1 cut(s) 3294
BtgZI GCGATG 1 cut(s) 2206
BtsCI GGATG 6 cut(s) 185, 503, 647, 717, 1418, 1845
BtsIMutI CAGTG 1 cut(s) 1458
BveI ACCTGC 1 cut(s) 2894
Cac8I GCNNGC 7 cut(s) 623, 627, 649, 653, 1226, 1332, 1877
CaiI CAGNNNCTG 1 cut(s) 2408
CciI TCATGA 4 cut(s) 2286, 2718, 2761, 3280
CfoI GCGC 2 cut(s) 1484, 1786
Cfr10I RCCGGY 3 cut(s) 1224, 1985, 2341
Cfr13I GGNCC 5 cut(s) 473, 2060, 2135, 2454, 2743
CseI GACGC 1 cut(s) 2282
CsiI ACCWGGT 1 cut(s) 3045
CspAI ACCGGT 1 cut(s) 1985
DinI GGCGCC 1 cut(s) 1483
DpnI GATC 9 cut(s) 80, 129, 138, 983, 1160, 1595, 1674, 2780, 2867
DpnII GATC 9 cut(s) 78, 127, 136, 981, 1158, 1593, 1672, 2778, 2865
DraIII CACNNNGTG 1 cut(s) 176
Eam1104I CTCTTC 3 cut(s) 480, 2341, 2434
EarI CTCTTC 3 cut(s) 480, 2341, 2434
Ecl136II GAGCTC 2 cut(s) 706, 994
Eco130I CCWWGG 3 cut(s) 1210, 2477, 3294
Eco147I AGGCCT 1 cut(s) 1209
Eco24I GRGCYC 2 cut(s) 708, 996
Eco32I GATATC 1 cut(s) 612
Eco47I GGWCC 2 cut(s) 2135, 2454
Eco53kI GAGCTC 2 cut(s) 706, 994
Eco57I CTGAAG 1 cut(s) 3075
Eco81I CCTNAGG 1 cut(s) 699
Eco88I CYCGRG 2 cut(s) 358, 707
Eco91I GGTNACC 1 cut(s) 3041
EcoICRI GAGCTC 2 cut(s) 706, 994
EcoO109I RGGNCCY 3 cut(s) 473, 2060, 2743
EcoO65I GGTNACC 1 cut(s) 3041
EcoRI GAATTC 2 cut(s) 546, 1955
EcoRII CCWGG 3 cut(s) 1996, 2619, 3045
EcoRV GATATC 1 cut(s) 612
EcoT14I CCWWGG 3 cut(s) 1210, 2477, 3294
EcoT22I ATGCAT 2 cut(s) 1702, 2938
EcoT38I GRGCYC 2 cut(s) 708, 996
EgeI GGCGCC 1 cut(s) 1483
EheI GGCGCC 1 cut(s) 1483
ErhI CCWWGG 3 cut(s) 1210, 2477, 3294
FalI AAGNNNNNCTT 2 cut(s) 2431, 2463
FaqI GGGAC 6 cut(s) 496, 919, 1423, 2280, 2467, 3172
FauI CCCGC 2 cut(s) 589, 2213
FauNDI CATATG 1 cut(s) 16
FbaI TGATCA 2 cut(s) 136, 1158
FblI GTMKAC 1 cut(s) 2023
FokI GGATG 6 cut(s) 192, 490, 654, 724, 1405, 1832
FriOI GRGCYC 2 cut(s) 708, 996
FspBI CTAG 7 cut(s) 104, 374, 423, 668, 938, 1388, 1563
GlaI GCGC 2 cut(s) 1483, 1785
GsaI CCCAGC 4 cut(s) 517, 1338, 1790, 1853
GsuI CTGGAG 2 cut(s) 890, 3014
HaeII RGCGCY 1 cut(s) 1485
HaeIII GGCC 4 cut(s) 475, 1209, 2062, 2745
HgaI GACGC 1 cut(s) 2282
HhaI GCGC 2 cut(s) 1484, 1786
Hin1I GRCGYC 2 cut(s) 1482, 2293
Hin6I GCGC 2 cut(s) 1482, 1784
HinP1I GCGC 2 cut(s) 1482, 1784
HincII GTYRAC 4 cut(s) 684, 1240, 1441, 2101
HindII GTYRAC 4 cut(s) 684, 1240, 1441, 2101
HindIII AAGCTT 3 cut(s) 619, 824, 1745
HpaI GTTAAC 2 cut(s) 684, 1441
HphI GGTGA 8 cut(s) 16, 281, 1603, 2000, 2156, 2203, 2777, 3053
HpyAV CCTTC 7 cut(s) 1620, 1970, 2497, 2999, 3029, 3122, 3245
HpyCH4IV ACGT 2 cut(s) 3053, 3185
HpyF10VI GCNNNNNNNGC 7 cut(s) 703, 712, 1359, 1525, 1744, 2231, 2732
HpySE526I ACGT 2 cut(s) 3053, 3185
Hsp92I GRCGYC 2 cut(s) 1482, 2293
HspAI GCGC 2 cut(s) 1482, 1784
KasI GGCGCC 1 cut(s) 1481
Kpn2I TCCGGA 3 cut(s) 614, 1492, 2182
KroI GCCGGC 1 cut(s) 1224
KroNI GCCGGC 1 cut(s) 1226
Ksp22I TGATCA 2 cut(s) 136, 1158
KspAI GTTAAC 2 cut(s) 684, 1441
Kzo9I GATC 9 cut(s) 78, 127, 136, 981, 1158, 1593, 1672, 2778, 2865
LmnI GCTCC 7 cut(s) 703, 775, 847, 1735, 2444, 2526, 2995
LweI GCATC 7 cut(s) 702, 1362, 1687, 1854, 2590, 2767, 3094
MabI ACCWGGT 1 cut(s) 3045
MaeI CTAG 7 cut(s) 104, 374, 423, 668, 938, 1388, 1563
MaeII ACGT 2 cut(s) 3053, 3185
MaeIII GTNAC 8 cut(s) 746, 1502, 1812, 2073, 2950, 2963, 3041, 3049
MalI GATC 9 cut(s) 80, 129, 138, 983, 1160, 1595, 1674, 2780, 2867
MbiI CCGCTC 1 cut(s) 556
MboI GATC 9 cut(s) 78, 127, 136, 981, 1158, 1593, 1672, 2778, 2865
MfeI CAATTG 6 cut(s) 784, 1016, 1169, 1648, 1730, 2433
MflI RGATCY 2 cut(s) 981, 2778
MhlI GDGCHC 4 cut(s) 708, 996, 1867, 3179
Mly113I GGCGCC 1 cut(s) 1482
MlyI GAGTC 2 cut(s) 319, 2029
MmeI TCCRAC 2 cut(s) 1372, 2538
Mph1103I ATGCAT 2 cut(s) 1702, 2938
MroI TCCGGA 3 cut(s) 614, 1492, 2182
MroNI GCCGGC 1 cut(s) 1224
MroXI GAANNNNTTC 6 cut(s) 499, 1040, 1472, 1723, 1959, 2676
MspA1I CMGCKG 3 cut(s) 517, 1479, 1853
MspR9I CCNGG 4 cut(s) 760, 1998, 2621, 3047
MunI CAATTG 6 cut(s) 784, 1016, 1169, 1648, 1730, 2433
Mva1269I GAATGC 1 cut(s) 2153
MvaI CCWGG 3 cut(s) 1998, 2621, 3047
MwoI GCNNNNNNNGC 7 cut(s) 703, 712, 1359, 1525, 1744, 2231, 2732
NaeI GCCGGC 1 cut(s) 1226
NarI GGCGCC 1 cut(s) 1482
NciI CCSGG 1 cut(s) 760
NcoI CCATGG 1 cut(s) 3294
NdeI CATATG 1 cut(s) 16
NdeII GATC 9 cut(s) 78, 127, 136, 981, 1158, 1593, 1672, 2778, 2865
NgoMIV GCCGGC 1 cut(s) 1224
NlaIV GGNNCC 5 cut(s) 1483, 1864, 2061, 2455, 2626
NmuCI GTSAC 3 cut(s) 2950, 2963, 3041
NsiI ATGCAT 2 cut(s) 1702, 2938
NspI RCATGY 1 cut(s) 2875
NspV TTCGAA 1 cut(s) 66
PaeR7I CTCGAG 1 cut(s) 358
PagI TCATGA 4 cut(s) 2286, 2718, 2761, 3280
PceI AGGCCT 1 cut(s) 1209
PctI GAATGC 1 cut(s) 2153
PdiI GCCGGC 1 cut(s) 1226
PdmI GAANNNNTTC 6 cut(s) 499, 1040, 1472, 1723, 1959, 2676
PfeI GAWTC 9 cut(s) 68, 154, 247, 838, 1137, 1462, 1939, 2011, 3269
PflFI GACNNNGTC 1 cut(s) 2890
PflMI CCANNNNNTGG 1 cut(s) 2395
PinAI ACCGGT 1 cut(s) 1985
PleI GAGTC 2 cut(s) 319, 2028
PluTI GGCGCC 1 cut(s) 1485
PpsI GAGTC 2 cut(s) 319, 2028
PshBI ATTAAT 3 cut(s) 734, 1107, 3150
Psp124BI GAGCTC 2 cut(s) 708, 996
Psp6I CCWGG 3 cut(s) 1996, 2619, 3045
PspEI GGTNACC 1 cut(s) 3041
PspFI CCCAGC 4 cut(s) 513, 1334, 1786, 1849
PspGI CCWGG 3 cut(s) 1996, 2619, 3045
PspN4I GGNNCC 5 cut(s) 1483, 1864, 2061, 2455, 2626
PspPI GGNCC 5 cut(s) 473, 2060, 2135, 2454, 2743
PspXI VCTCGAGB 1 cut(s) 358
PstI CTGCAG 6 cut(s) 631, 1289, 1511, 1881, 2377, 2899
PstNI CAGNNNCTG 1 cut(s) 2408
PsuI RGATCY 2 cut(s) 981, 2778
PsyI GACNNNGTC 1 cut(s) 2890
PvuII CAGCTG 2 cut(s) 517, 1853
SacI GAGCTC 2 cut(s) 708, 996
Sau3AI GATC 9 cut(s) 78, 127, 136, 981, 1158, 1593, 1672, 2778, 2865
Sau96I GGNCC 5 cut(s) 473, 2060, 2135, 2454, 2743
ScaI AGTACT 3 cut(s) 444, 1145, 3099
SchI GAGTC 2 cut(s) 319, 2029
ScrFI CCNGG 4 cut(s) 760, 1998, 2621, 3047
SduI GDGCHC 4 cut(s) 708, 996, 1867, 3179
SexAI ACCWGGT 1 cut(s) 3045
SfaNI GCATC 7 cut(s) 702, 1362, 1687, 1854, 2590, 2767, 3094
SfcI CTRYAG 8 cut(s) 627, 1285, 1449, 1507, 1877, 2373, 2895, 2971
SfoI GGCGCC 1 cut(s) 1483
Sfr274I CTCGAG 1 cut(s) 358
SfuI TTCGAA 1 cut(s) 66
SinI GGWCC 2 cut(s) 2135, 2454
SlaI CTCGAG 1 cut(s) 358
SmlI CTYRAG 4 cut(s) 358, 716, 1203, 1742
SmoI CTYRAG 4 cut(s) 358, 716, 1203, 1742
SseBI AGGCCT 1 cut(s) 1209
SspDI GGCGCC 1 cut(s) 1481
SspI AATATT 1 cut(s) 2590
SspMI CTAG 7 cut(s) 104, 374, 423, 668, 938, 1388, 1563
SstI GAGCTC 2 cut(s) 708, 996
StuI AGGCCT 1 cut(s) 1209
StyD4I CCNGG 4 cut(s) 758, 1996, 2619, 3045
StyI CCWWGG 3 cut(s) 1210, 2477, 3294
TaiI ACGT 2 cut(s) 3056, 3188
TatI WGTACW 8 cut(s) 442, 788, 1143, 2355, 2460, 2646, 3019, 3097
TauI GCSGC 2 cut(s) 556, 1482
TfiI GAWTC 9 cut(s) 68, 154, 247, 838, 1137, 1462, 1939, 2011, 3269
TscAI CASTG 1 cut(s) 1458
TseFI GTSAC 3 cut(s) 2950, 2963, 3041
Tsp45I GTSAC 3 cut(s) 2950, 2963, 3041
TspDTI ATGAA 9 cut(s) 260, 333, 492, 498, 2304, 2669, 2707, 3141, 3261
TspGWI ACGGA 2 cut(s) 219, 2994
TspRI CASTG 1 cut(s) 1458
Tth111I GACNNNGTC 1 cut(s) 2890
Van91I CCANNNNNTGG 1 cut(s) 2395
VneI GTGCAC 1 cut(s) 3175
VpaK11BI GGWCC 2 cut(s) 2135, 2454
VspI ATTAAT 3 cut(s) 734, 1107, 3150
XbaI TCTAGA 1 cut(s) 103
XceI RCATGY 1 cut(s) 2875
XcmI CCANNNNNNNNNTGG 1 cut(s) 488
XhoI CTCGAG 1 cut(s) 358
XmiI GTMKAC 1 cut(s) 2023
XmnI GAANNNNTTC 6 cut(s) 499, 1040, 1472, 1723, 1959, 2676
XspI CTAG 7 cut(s) 104, 374, 423, 668, 938, 1388, 1563
ZrmI AGTACT 3 cut(s) 444, 1145, 3099
Zsp2I ATGCAT 2 cut(s) 1702, 2938
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.