RchiOBHm_Chr3g0448891
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0011826
Physical Location & Seq
Reverse (-)
919956 .. 923873
3918 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41631

Sequence Viewer

Length: 3453 bp
ATGATTTTTCCCAGTAAATTTTCTTTTTCTGGGAAACTGAAGTATATTTCTTGTTGGTTCCTAGAGAACTGGTTGGCTCTCTTTGATGGCTTCTTTTTGTTTTCCATTCTTGGTCATCAACTTGAGATATCAGTGGGTTTGCTAATTACTGTAGTTGCTCTTGTTACTGCCCTGATGGTTTATGAATCTGAGGGGCTTAATGATCAGTGTCAATACCTTCTAGAAATAAAGAGTAGAATTGTTGACGGATTTGGCCATCTTAGTAGTTGGAATTCCAATGATTCAACACCTTGTGGATGGAGGGGAGTGGATTGTAGCGAAGGATTGTATCCGGTGGTCTTGTCTCTTGACTTGAGTTCCATGAATCTTTCGGGTTCTTTATCTCCAAGCATTGGTAGAATGATTCACCTGAATTATCTTGACCTTTCGTTCAATGAAATGTCTGGGAGTATACCTGAAGACATTGGGAATTGTTCCTATTTGCAGGTTCTTAGTTTAAATAACAATAAGTTTGAAGGTCAAATTCCCACAGGATTGGGTAGACTTTCTTATTTAGAAGTCTTGAATGTGTGCAATAATAGAATATCAGGGCCTTTCCCAGAAGAAGTTGGAAATCTTTCTTCTCTGTCGCAATTGATTGCATACACTAATAATCTTAGTGGACCACTGCCGCGTTCTATCGGCAACCTCAAAAGATTGAAGACTTTCCGGGCAGGGGAGAATTCAATATCAGGAAGCTTACCTTCCGAGATTGGTGAATGTGAAAGCTTGAACTATCTTGGTCTAGCTCAAAATCAGTTAAGCGGAGGAATTCCGAAAGAGATTGGAATGCTCGAGAATTTGAGTGTTTTAATTCTTTGGGAAAATCTGCTTTCTGGTGTCATTCCAAAGGAGCTTGGCAACTGTACAAATTTGTATACTCTGGCCCTCTACAAGAACAAGCTTGAAGGAGAGATACCAAAGGAGCTTGGGAGTCTTGTATTTCTAGAGAAGCTATATGTGTACAGAAATATGCTGAATGGGACAGTTCCAAGAGAGATTGGTAATCTTTCTCTTGCTACGGAGATTGATTTTTCTGAGAACTTGTTGTCCGGAGAGATACCAGTTGAGCTCAGTAAGATATCGGGTTTGCAATTGCTTTATCTTTTCGAAAACCATCTCACAGGTGTTATTCCAGAGGAGCTCACAAGCTTGACAAACCTGACAAAGCTCGACCTGTCTATAAATTTTCTCACTGGTTCAATTCCAACTGGATTTCAATACATGAATGAGCTGATCATGCTACAGTTGTTTAACAATAGGTTGAGTGACATAATTCCTCAAGGACTTGGGGTGTACAGCCCACTTTGGGTGGTTGACTTATCTGAGAACTTCCTGACAGGAAGAATTCCCCGTCATGTCTGCAGAAATTCTAATCTGATTTTGTTAAACTTGGGGTCTAACAGGCTCACCGGTAATATCCCAACTGGGGTCACAAGCTGCAAGTCATTGGCGCAGCTCCGTCTGGTGGGAAACAACCTTACAGGGACTCTTCCAACTGAAATGTGCAAATTGGTTAACCTTTCTGCAGTTGAGTTGGATCAAAACAAGTTCAGCGGTGCGATTCCTCCAGCAATTGGTAGCTGCAGAACTTTGCAGAGACTACTACTTGCAGACAACTACTTCACATCTGAGTTGCCAAGAGAGATTGGTGAGCTCTCGCAGTTGGTGATCTTCAATATCTCATCGAATTTACTTTCTGGCAGGATACCACCTGAAATTTTCAATTGTAAAATGCTTCAACGGCTTGATCTGAGTAGCAACAACTTTTCGGATGTCTTGCCAAGTGAGATAGGAGCACTTTCTCAGTTGGAGCTTCTCAAGCTCTCCGATAATCATCTTTCGGGGGATATCCCAGTTGCACTGGGAAATCTCTTGCATTTGACTGAGCTACAAATGGGTGGCAACTCATTCTCTGGGGGTATACCAGCTGAGTTGGGTGCCCTTTCAAGCCTCCAGATTGGATTAAATCTCAGTTATAACAATATTTCTGGGGAGATACCTCCTCAACTTGGAAATCTTATATTACTGGAACTGCTTCTACTCGATAACAACCATTTGACTGGTGGAATTCCAGGTTCTTTTGACAACCTGTCAAGTTTACTTGGCTGCAACTTTTCATACAATGACTTGATAGGGCCTATTCCTTCTCTACCACTCTTTCAGAGTATGGATGTTAGCAGCTTTGTTGGGAATAAAAGACTTTGCGGTGGCCCTCTTGGTGCTTGTGAAACACCATCTTCACTCTCTTTTCCTCCAAATGTGGCAAACAAAAGAACCCGCTTAGGGAAGATTGTTGCTATTGTTGCGGCAGCCATTGGTGGTGTTTCTCTTATCTTAATCGTAATCATTATATATGTGATGAGACGTCCTGTAGACAATGTAGTAACTTCTGCGCAAGAAAAACCACTCTCTTCTCCAGTTTTAGATGTGTACTTTTCGCCAAAGGCAGGATTCACTTTTCAAGACTTGGTTATGGCGACTGAAAACTTTGACGAGAGATTTGCAATTGGAAGGGGAGCTTCTGGAACTGTGTATAAAGCCGTCTTGTTTCACGGTCACACAGTTGCTGTCAAGAAGGTAGTATCCAACAGGGAAGGAAACAATGTGGATAGTAGCTTTCATGCAGAGATTCTAACTTTAGGAAATATCAGGCATCGAAATATTGTGAAGCTGTATGGTTTCTGCTACCACCAGGGTTCCAATCTTCTGCTTTATGAGTACATGGAAAGGGGCAGCTTAGGAGAATTACTTCATGGAAAATCTTGTAATCTTGATTGGCAAACGAGGTTCAATATTGCTCTTGGGGCTGCCCAGGGTCTCTCTTATCTGCATCATGATTGCAAACCTCGGATCTTTCACCGTGATATAAAGTCCAACAACATTCTCCTCGATGAAATGTTTGAAGCTCATGTTGGAGATTTTGGGTTAGCAAAGGTGATTGACATGCCCTATTCGAAGTCAATGTCTGCAGTAGCAGGTTCTTATGGATACATAGCCCCTGAGTATGCATACACTATGAAAGTCACAGAAAAATGTGACATCTACAGCTATGGAGTGGTCCTTCTGGAGTTGCTGACTGGTAAAACACCAGTACAATCATTAGACGAAGGCGGCGACCTGGTAACTTGGGTTAGAACATACTTTCTCAACAATTCATTGTCATCAGAAGTACTCGATGCTCGATTGAATTTACAAGATGAAGATGAAGCTGAAGCTGCTGCTACTGTCTCTCACATGATTACTGTCTTGAAAATTGCTTTGTTGTGCACAAGCATGTCTCCTTTTGAACGCCCGACCATGAGAGAAGTTGTTTCCATGCTTCTTGGGTCGAATGAGAAAGAAACAGTACAGTTTGCTAATGAGTCCCCAAGTGATGATACTGATACGAATTCGACTTCTAAGATGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

1150

Amino Acids

125.86

Weight (kDa)

5.1

Isoelectric Point (pI)

35.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 66 - 106 2.4e-11 Leucine rich repeat N-terminal domain
LRR_14 PF23598 113 - 214 2.7e-11 Leucine-rich repeat region
LRR_14 PF23598 222 - 311 1.3e-07 Leucine-rich repeat region
LRR_14 PF23598 514 - 622 5.6e-07 Leucine-rich repeat region
LRR_8 PF13855 568 - 627 8.7e-06 Leucine rich repeat
LRR_14 PF23598 582 - 666 1.2e-07 Leucine-rich repeat region
LRR_8 PF13855 593 - 650 4.6e-06 Leucine rich repeat
Pkinase PF00069 849 - 1119 4.7e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 849 - 1121 1.1e-43 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011826)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G63930 AT5G63930
fragaria_vesca FvH4_6g01320 FvH4_6g01320
malus_domestica MD04G1235000.v1.1 MD12G1253000.v1.1
prunus_persica Prupe.6G354900_v2.0.a1 Prupe.6G354900_v2.0.a1
pyrus_communis pycom04g20870 pycom12g23210
rosa_chinensis RchiOBHm_Chr3g0448891
rosa_laevigata RLG00000025869
rosa_multiflora Rmu_co8340729.1_g000001 Rmu_sc0003116.1_g000020
rosa_rugosa Rorug02G0617000
rosa_samantha Rh3AG015200 Rh3CG013900 Rh3DG015500
rosa_wichuraiana Rw3G001130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2019
AatII GACGTC 1 cut(s) 2410
Acc16I TGCGCA 1 cut(s) 2436
Acc36I ACCTGC 2 cut(s) 475, 3008
AccB1I GGYRCC 1 cut(s) 1979
AccB7I CCANNNNNTGG 2 cut(s) 392, 1616
AccI GTMKAC 5 cut(s) 451, 541, 917, 1963, 2415
AccII CGCG 1 cut(s) 673
AccIII TCCGGA 1 cut(s) 1091
AciI CCGC 7 cut(s) 671, 804, 1596, 2247, 2320, 2348, 3153
AclWI GGATC 2 cut(s) 1587, 2900
AcoI YGGCCR 1 cut(s) 253
AcuI CTGAAG 3 cut(s) 59, 477, 3273
AcyI GRCGYC 1 cut(s) 2407
AdeI CACNNNGTG 1 cut(s) 293
AfaI GTAC 9 cut(s) 907, 1004, 1337, 2474, 2762, 3135, 3213, 3390, 3449
AfiI CCNNNNNNNGG 9 cut(s) 392, 715, 1348, 1468, 1507, 1616, 2051, 2325, 2489
AgeI ACCGGT 1 cut(s) 1451
AhdI GACNNNNNGTC 1 cut(s) 2131
AjnI CCWGG 4 cut(s) 2113, 2733, 2853, 3159
AjuI GAANNNNNNNTTGG 2 cut(s) 2937, 2969
AloI GAACNNNNNNTCC 2 cut(s) 1073, 1105
Alw21I GWGCWC 5 cut(s) 1113, 1185, 1698, 1840, 3311
Alw26I GTCTC 6 cut(s) 348, 1633, 2398, 2864, 3274, 3324
Alw44I GTGCAC 1 cut(s) 3307
AlwI GGATC 2 cut(s) 1587, 2900
AlwNI CAGNNNCTG 1 cut(s) 2609
Ama87I CYCGRG 1 cut(s) 833
Aor13HI TCCGGA 1 cut(s) 1091
AoxI GGCC 5 cut(s) 253, 590, 924, 2177, 2251
ApaLI GTGCAC 1 cut(s) 3307
ArsI GACNNNNNNTTYG 4 cut(s) 2525, 2557, 2990, 3022
AsiGI ACCGGT 1 cut(s) 1451
Asp700I GAANNNNTTC 4 cut(s) 616, 704, 2076, 2790
AspLEI GCGC 2 cut(s) 1495, 2437
AspS9I GGNCC 6 cut(s) 590, 662, 925, 2177, 2252, 3100
AsuC2I CCSGG 1 cut(s) 710
AsuHPI GGTGA 7 cut(s) 398, 767, 1441, 1703, 1720, 2891, 2989
AsuII TTCGAA 2 cut(s) 1149, 2996
AvaI CYCGRG 1 cut(s) 833
AvaII GGWCC 2 cut(s) 662, 3100
BaeGI GKGCMC 2 cut(s) 1984, 3311
BalI TGGCCA 1 cut(s) 255
BanI GGYRCC 1 cut(s) 1979
BanII GRGCYC 3 cut(s) 1113, 1185, 1698
BarI GAAGNNNNNNTAC 2 cut(s) 939, 971
BbsI GAAGAC 2 cut(s) 465, 707
Bbv12I GWGCWC 5 cut(s) 1113, 1185, 1698, 1840, 3311
BccI CCATC 6 cut(s) 80, 169, 264, 291, 1164, 2284
BceAI ACGGC 2 cut(s) 1799, 2567
BcgI CGANNNNNNTGC 2 cut(s) 2525, 2559
BciT130I CCWGG 4 cut(s) 2115, 2735, 2855, 3161
BciVI GTATCC 4 cut(s) 339, 1740, 2635, 3023
BclI TGATCA 2 cut(s) 202, 1275
BcnI CCSGG 1 cut(s) 710
BcoDI GTCTC 6 cut(s) 348, 1633, 2398, 2864, 3274, 3324
BfaI CTAG 4 cut(s) 62, 221, 785, 986
BfmI CTRYAG 8 cut(s) 150, 1283, 1402, 1566, 1624, 2412, 3009, 3085
BfuAI ACCTGC 2 cut(s) 475, 3008
BfuI GTATCC 4 cut(s) 339, 1740, 2635, 3023
BmcAI AGTACT 1 cut(s) 3213
Bme1390I CCNGG 5 cut(s) 710, 2115, 2735, 2855, 3161
Bme18I GGWCC 2 cut(s) 662, 3100
BmeRI GACNNNNNGTC 1 cut(s) 2131
BmeT110I CYCGRG 1 cut(s) 833
BmgT120I GGNCC 6 cut(s) 590, 662, 925, 2177, 2252, 3100
BmiI GGNNCC 3 cut(s) 59, 1981, 2740
BmrFI CCNGG 5 cut(s) 710, 2115, 2735, 2855, 3161
BmrI ACTGGG 4 cut(s) 6, 1476, 1889, 1913
BmsI GCATC 3 cut(s) 2704, 2881, 3208
BmuI ACTGGG 4 cut(s) 6, 1476, 1889, 1913
BpiI GAAGAC 2 cut(s) 465, 707
BpmI CTGGAG 4 cut(s) 1593, 1979, 2442, 3128
Bpu10I CCTNAGC 2 cut(s) 2323, 2779
Bpu14I TTCGAA 2 cut(s) 1149, 2996
BpuEI CTTGAG 4 cut(s) 143, 373, 1305, 1844
BpuMI CCSGG 1 cut(s) 710
BsaBI GATNNNNATC 1 cut(s) 1875
BsaHI GRCGYC 1 cut(s) 2407
BsaI GGTCTC 1 cut(s) 2864
BsaJI CCNNGG 4 cut(s) 2734, 2853, 2854, 2888
BsaWI WCCGGW 3 cut(s) 331, 1091, 1451
Bsc4I CCNNNNNNNGG 9 cut(s) 392, 715, 1348, 1468, 1507, 1616, 2051, 2325, 2489
Bse118I RCCGGY 1 cut(s) 1451
Bse8I GATNNNNATC 1 cut(s) 1875
BseAI TCCGGA 1 cut(s) 1091
BseBI CCWGG 4 cut(s) 2115, 2735, 2855, 3161
BseDI CCNNGG 4 cut(s) 2734, 2853, 2854, 2888
BseGI GGATG 3 cut(s) 302, 1819, 2218
BseJI GATNNNNATC 1 cut(s) 1875
BseLI CCNNNNNNNGG 9 cut(s) 392, 715, 1348, 1468, 1507, 1616, 2051, 2325, 2489
BseRI GAGGAG 3 cut(s) 1193, 2034, 2918
BseSI GKGCMC 2 cut(s) 1984, 3311
Bsh1236I CGCG 1 cut(s) 673
BshFI GGCC 5 cut(s) 255, 592, 926, 2179, 2253
BshNI GGYRCC 1 cut(s) 1979
BshTI ACCGGT 1 cut(s) 1451
BsiHKAI GWGCWC 5 cut(s) 1113, 1185, 1698, 1840, 3311
BsiHKCI CYCGRG 1 cut(s) 833
BsiSI CCGG 4 cut(s) 332, 709, 1092, 1452
BslFI GGGAC 3 cut(s) 1036, 1540, 3391
BslI CCNNNNNNNGG 9 cut(s) 392, 715, 1348, 1468, 1507, 1616, 2051, 2325, 2489
BsmAI GTCTC 6 cut(s) 348, 1633, 2398, 2864, 3274, 3324
BsmBI CGTCTC 1 cut(s) 2398
BsmFI GGGAC 3 cut(s) 1036, 1540, 3391
BsmI GAATGC 1 cut(s) 834
BsnI GGCC 5 cut(s) 255, 592, 926, 2179, 2253
Bso31I GGTCTC 1 cut(s) 2864
BsoBI CYCGRG 1 cut(s) 833
Bsp119I TTCGAA 2 cut(s) 1149, 2996
Bsp1286I GDGCHC 6 cut(s) 1113, 1185, 1698, 1840, 1984, 3311
Bsp13I TCCGGA 1 cut(s) 1091
Bsp1407I TGTACA 3 cut(s) 905, 1002, 1335
Bsp143I GATC 6 cut(s) 202, 1275, 1579, 1710, 1789, 2892
BspACI CCGC 7 cut(s) 671, 804, 1596, 2247, 2320, 2348, 3153
BspANI GGCC 5 cut(s) 255, 592, 926, 2179, 2253
BspEI TCCGGA 1 cut(s) 1091
BspFNI CGCG 1 cut(s) 673
BspHI TCATGA 1 cut(s) 2875
BspLI GGNNCC 3 cut(s) 59, 1981, 2740
BspMAI CTGCAG 4 cut(s) 1406, 1570, 1628, 3013
BspMI ACCTGC 2 cut(s) 475, 3008
BspPI GGATC 2 cut(s) 1587, 2900
BspT104I TTCGAA 2 cut(s) 1149, 2996
BspT107I GGYRCC 1 cut(s) 1979
BspTNI GGTCTC 1 cut(s) 2864
BsrFI RCCGGY 1 cut(s) 1451
BsrGI TGTACA 3 cut(s) 905, 1002, 1335
BssAI RCCGGY 1 cut(s) 1451
BssECI CCNNGG 4 cut(s) 2734, 2853, 2854, 2888
BssMI GATC 6 cut(s) 202, 1275, 1579, 1710, 1789, 2892
BssNAI GTATAC 3 cut(s) 452, 918, 1964
BssNI GRCGYC 1 cut(s) 2407
Bst1107I GTATAC 3 cut(s) 452, 918, 1964
Bst2UI CCWGG 4 cut(s) 2115, 2735, 2855, 3161
Bst6I CTCTTC 2 cut(s) 1536, 2458
BstACI GRCGYC 1 cut(s) 2407
BstAUI TGTACA 3 cut(s) 905, 1002, 1335
BstBI TTCGAA 2 cut(s) 1149, 2996
BstF5I GGATG 3 cut(s) 302, 1819, 2218
BstFNI CGCG 1 cut(s) 673
BstHHI GCGC 2 cut(s) 1495, 2437
BstKTI GATC 6 cut(s) 205, 1278, 1582, 1713, 1792, 2895
BstMAI GTCTC 6 cut(s) 348, 1633, 2398, 2864, 3274, 3324
BstMBI GATC 6 cut(s) 202, 1275, 1579, 1710, 1789, 2892
BstMWI GCNNNNNNNGC 6 cut(s) 1279, 1783, 1861, 2345, 2846, 3257
BstNI CCWGG 4 cut(s) 2115, 2735, 2855, 3161
BstNSI RCATGY 2 cut(s) 2989, 3319
BstSCI CCNGG 5 cut(s) 708, 2113, 2733, 2853, 3159
BstSFI CTRYAG 8 cut(s) 150, 1283, 1402, 1566, 1624, 2412, 3009, 3085
BstSLI GKGCMC 2 cut(s) 1984, 3311
BstUI CGCG 1 cut(s) 673
BstV2I GAAGAC 2 cut(s) 465, 707
BstX2I RGATCY 1 cut(s) 2892
BstXI CCANNNNNNTGG 2 cut(s) 535, 2102
BstYI RGATCY 1 cut(s) 2892
BstZ17I GTATAC 3 cut(s) 452, 918, 1964
BsuI GTATCC 4 cut(s) 339, 1740, 2635, 3023
BsuRI GGCC 5 cut(s) 255, 592, 926, 2179, 2253
BtsCI GGATG 3 cut(s) 302, 1819, 2218
BtsI GCAGTG 1 cut(s) 665
BtsIMutI CAGTG 5 cut(s) 138, 212, 665, 1233, 1901
BveI ACCTGC 2 cut(s) 475, 3008
CaiI CAGNNNCTG 1 cut(s) 2609
CciI TCATGA 1 cut(s) 2875
CfoI GCGC 2 cut(s) 1495, 2437
Cfr10I RCCGGY 1 cut(s) 1451
Cfr13I GGNCC 6 cut(s) 590, 662, 925, 2177, 2252, 3100
CsiI ACCWGGT 1 cut(s) 3159
Csp6I GTAC 9 cut(s) 906, 1003, 1336, 2473, 2761, 3134, 3212, 3389, 3448
CspAI ACCGGT 1 cut(s) 1451
CviQI GTAC 9 cut(s) 906, 1003, 1336, 2473, 2761, 3134, 3212, 3389, 3448
DpnI GATC 6 cut(s) 204, 1277, 1581, 1712, 1791, 2894
DpnII GATC 6 cut(s) 202, 1275, 1579, 1710, 1789, 2892
DraI TTTAAA 1 cut(s) 498
DraIII CACNNNGTG 1 cut(s) 293
DriI GACNNNNNGTC 1 cut(s) 2131
EaeI YGGCCR 1 cut(s) 253
Eam1104I CTCTTC 2 cut(s) 1536, 2458
Eam1105I GACNNNNNGTC 1 cut(s) 2131
EarI CTCTTC 2 cut(s) 1536, 2458
Ecl136II GAGCTC 3 cut(s) 1111, 1183, 1696
Eco24I GRGCYC 3 cut(s) 1113, 1185, 1698
Eco31I GGTCTC 1 cut(s) 2864
Eco32I GATATC 3 cut(s) 129, 1122, 1891
Eco47I GGWCC 2 cut(s) 662, 3100
Eco53kI GAGCTC 3 cut(s) 1111, 1183, 1696
Eco57I CTGAAG 3 cut(s) 59, 477, 3273
Eco88I CYCGRG 1 cut(s) 833
EcoICRI GAGCTC 3 cut(s) 1111, 1183, 1696
EcoO109I RGGNCCY 2 cut(s) 590, 2177
EcoRI GAATTC 6 cut(s) 271, 721, 810, 1386, 2109, 3430
EcoRII CCWGG 4 cut(s) 2113, 2733, 2853, 3159
EcoRV GATATC 3 cut(s) 129, 1122, 1891
EcoT22I ATGCAT 1 cut(s) 3052
EcoT38I GRGCYC 3 cut(s) 1113, 1185, 1698
Esp3I CGTCTC 1 cut(s) 2398
FalI AAGNNNNNCTT 4 cut(s) 727, 759, 2545, 2577
FaqI GGGAC 3 cut(s) 1036, 1540, 3391
FauI CCCGC 1 cut(s) 2327
FbaI TGATCA 2 cut(s) 202, 1275
FblI GTMKAC 5 cut(s) 451, 541, 917, 1963, 2415
FokI GGATG 3 cut(s) 309, 1826, 2225
FriOI GRGCYC 3 cut(s) 1113, 1185, 1698
FspBI CTAG 4 cut(s) 62, 221, 785, 986
FspI TGCGCA 1 cut(s) 2436
GlaI GCGC 2 cut(s) 1494, 2436
GsuI CTGGAG 4 cut(s) 1593, 1979, 2442, 3128
HaeIII GGCC 5 cut(s) 255, 592, 926, 2179, 2253
HapII CCGG 4 cut(s) 332, 709, 1092, 1452
HhaI GCGC 2 cut(s) 1495, 2437
Hin1I GRCGYC 1 cut(s) 2407
Hin6I GCGC 2 cut(s) 1493, 2435
HinP1I GCGC 2 cut(s) 1493, 2435
HincII GTYRAC 3 cut(s) 244, 1357, 1558
HindII GTYRAC 3 cut(s) 244, 1357, 1558
HindIII AAGCTT 4 cut(s) 736, 766, 941, 1189
HpaI GTTAAC 1 cut(s) 1558
HpaII CCGG 4 cut(s) 332, 709, 1092, 1452
HphI GGTGA 7 cut(s) 398, 767, 1441, 1703, 1720, 2891, 2989
HpyCH4IV ACGT 1 cut(s) 2407
HpyF10VI GCNNNNNNNGC 6 cut(s) 1279, 1783, 1861, 2345, 2846, 3257
HpySE526I ACGT 1 cut(s) 2407
Hsp92I GRCGYC 1 cut(s) 2407
HspAI GCGC 2 cut(s) 1493, 2435
Kpn2I TCCGGA 1 cut(s) 1091
Ksp22I TGATCA 2 cut(s) 202, 1275
KspAI GTTAAC 1 cut(s) 1558
Kzo9I GATC 6 cut(s) 202, 1275, 1579, 1710, 1789, 2892
LmnI GCTCC 7 cut(s) 892, 964, 1180, 1503, 1835, 1852, 2558
LweI GCATC 3 cut(s) 2704, 2881, 3208
MabI ACCWGGT 1 cut(s) 3159
MaeI CTAG 4 cut(s) 62, 221, 785, 986
MaeII ACGT 1 cut(s) 2407
MaeIII GTNAC 8 cut(s) 163, 1307, 1471, 2425, 2597, 3064, 3077, 3163
MalI GATC 6 cut(s) 204, 1277, 1581, 1712, 1791, 2894
MboI GATC 6 cut(s) 202, 1275, 1579, 1710, 1789, 2892
MfeI CAATTG 5 cut(s) 632, 1133, 1614, 1765, 2547
MflI RGATCY 1 cut(s) 2892
MhlI GDGCHC 6 cut(s) 1113, 1185, 1698, 1840, 1984, 3311
MlsI TGGCCA 1 cut(s) 255
MluNI TGGCCA 1 cut(s) 255
MlyI GAGTC 3 cut(s) 982, 1522, 3413
MmeI TCCRAC 9 cut(s) 248, 589, 1271, 1557, 1559, 1830, 2652, 2935, 2940
Mox20I TGGCCA 1 cut(s) 255
Mph1103I ATGCAT 1 cut(s) 3052
MroI TCCGGA 1 cut(s) 1091
MroXI GAANNNNTTC 4 cut(s) 616, 704, 2076, 2790
MscI TGGCCA 1 cut(s) 255
MseI TTAA 9 cut(s) 198, 497, 800, 851, 1293, 1427, 1557, 2006, 2378
MslI CAYNNNNRTG 1 cut(s) 3314
Msp20I TGGCCA 1 cut(s) 255
MspA1I CMGCKG 2 cut(s) 1596, 1970
MspI CCGG 4 cut(s) 332, 709, 1092, 1452
MspR9I CCNGG 5 cut(s) 710, 2115, 2735, 2855, 3161
MunI CAATTG 5 cut(s) 632, 1133, 1614, 1765, 2547
Mva1269I GAATGC 1 cut(s) 834
MvaI CCWGG 4 cut(s) 2115, 2735, 2855, 3161
MvnI CGCG 1 cut(s) 673
MwoI GCNNNNNNNGC 6 cut(s) 1279, 1783, 1861, 2345, 2846, 3257
NciI CCSGG 1 cut(s) 710
NdeII GATC 6 cut(s) 202, 1275, 1579, 1710, 1789, 2892
NlaIV GGNNCC 3 cut(s) 59, 1981, 2740
NmuCI GTSAC 5 cut(s) 1307, 1471, 2597, 3064, 3077
NsbI TGCGCA 1 cut(s) 2436
NsiI ATGCAT 1 cut(s) 3052
NspI RCATGY 2 cut(s) 2989, 3319
NspV TTCGAA 2 cut(s) 1149, 2996
PaeR7I CTCGAG 1 cut(s) 833
PagI TCATGA 1 cut(s) 2875
PasI CCCWGGG 1 cut(s) 2854
PcsI WCGNNNNNNNCGW 1 cut(s) 3153
PctI GAATGC 1 cut(s) 834
PdmI GAANNNNTTC 4 cut(s) 616, 704, 2076, 2790
PfeI GAWTC 7 cut(s) 185, 281, 364, 403, 1603, 2493, 2671
PflMI CCANNNNNTGG 2 cut(s) 392, 1616
PinAI ACCGGT 1 cut(s) 1451
PleI GAGTC 3 cut(s) 981, 1522, 3412
PpsI GAGTC 3 cut(s) 981, 1522, 3412
PsiI TTATAA 1 cut(s) 2019
Psp124BI GAGCTC 3 cut(s) 1113, 1185, 1698
Psp6I CCWGG 4 cut(s) 2113, 2733, 2853, 3159
PspGI CCWGG 4 cut(s) 2113, 2733, 2853, 3159
PspN4I GGNNCC 3 cut(s) 59, 1981, 2740
PspPI GGNCC 6 cut(s) 590, 662, 925, 2177, 2252, 3100
PsrI GAACNNNNNNTAC 2 cut(s) 2064, 2096
PstI CTGCAG 4 cut(s) 1406, 1570, 1628, 3013
PstNI CAGNNNCTG 1 cut(s) 2609
PsuI RGATCY 1 cut(s) 2892
PvuII CAGCTG 1 cut(s) 1970
RsaI GTAC 9 cut(s) 907, 1004, 1337, 2474, 2762, 3135, 3213, 3390, 3449
RsaNI GTAC 9 cut(s) 906, 1003, 1336, 2473, 2761, 3134, 3212, 3389, 3448
RseI CAYNNNNRTG 1 cut(s) 3314
SacI GAGCTC 3 cut(s) 1113, 1185, 1698
SaqAI TTAA 9 cut(s) 198, 497, 800, 851, 1293, 1427, 1557, 2006, 2378
Sau3AI GATC 6 cut(s) 202, 1275, 1579, 1710, 1789, 2892
Sau96I GGNCC 6 cut(s) 590, 662, 925, 2177, 2252, 3100
ScaI AGTACT 1 cut(s) 3213
SchI GAGTC 3 cut(s) 982, 1522, 3413
ScrFI CCNGG 5 cut(s) 710, 2115, 2735, 2855, 3161
SduI GDGCHC 6 cut(s) 1113, 1185, 1698, 1840, 1984, 3311
SexAI ACCWGGT 1 cut(s) 3159
SfaNI GCATC 3 cut(s) 2704, 2881, 3208
SfcI CTRYAG 8 cut(s) 150, 1283, 1402, 1566, 1624, 2412, 3009, 3085
Sfr274I CTCGAG 1 cut(s) 833
SfuI TTCGAA 2 cut(s) 1149, 2996
SinI GGWCC 2 cut(s) 662, 3100
SlaI CTCGAG 1 cut(s) 833
SmiMI CAYNNNNRTG 1 cut(s) 3314
SmlI CTYRAG 5 cut(s) 122, 352, 833, 1320, 1859
SmoI CTYRAG 5 cut(s) 122, 352, 833, 1320, 1859
SsiI CCGC 7 cut(s) 671, 804, 1596, 2247, 2320, 2348, 3153
SspI AATATT 3 cut(s) 2026, 2704, 2836
SspMI CTAG 4 cut(s) 62, 221, 785, 986
SstI GAGCTC 3 cut(s) 1113, 1185, 1698
StyD4I CCNGG 5 cut(s) 708, 2113, 2733, 2853, 3159
TaiI ACGT 1 cut(s) 2410
TatI WGTACW 9 cut(s) 905, 1002, 1335, 2472, 2760, 3133, 3211, 3388, 3447
TauI GCSGC 3 cut(s) 673, 2351, 3156
TfiI GAWTC 7 cut(s) 185, 281, 364, 403, 1603, 2493, 2671
Tru1I TTAA 9 cut(s) 198, 497, 800, 851, 1293, 1427, 1557, 2006, 2378
Tru9I TTAA 9 cut(s) 198, 497, 800, 851, 1293, 1427, 1557, 2006, 2378
TscAI CASTG 5 cut(s) 138, 212, 672, 1240, 1908
TseFI GTSAC 5 cut(s) 1307, 1471, 2597, 3064, 3077
Tsp45I GTSAC 5 cut(s) 1307, 1471, 2597, 3064, 3077
TspGWI ACGGA 3 cut(s) 261, 1076, 1490
TspRI CASTG 5 cut(s) 138, 212, 672, 1240, 1908
Van91I CCANNNNNTGG 2 cut(s) 392, 1616
VneI GTGCAC 1 cut(s) 3307
VpaK11BI GGWCC 2 cut(s) 662, 3100
XbaI TCTAGA 2 cut(s) 220, 985
XceI RCATGY 2 cut(s) 2989, 3319
XcmI CCANNNNNNNNNTGG 1 cut(s) 2102
XhoI CTCGAG 1 cut(s) 833
XmiI GTMKAC 5 cut(s) 451, 541, 917, 1963, 2415
XmnI GAANNNNTTC 4 cut(s) 616, 704, 2076, 2790
XspI CTAG 4 cut(s) 62, 221, 785, 986
ZraI GACGTC 1 cut(s) 2408
ZrmI AGTACT 1 cut(s) 3213
Zsp2I ATGCAT 1 cut(s) 3052
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.