Rw3G001130
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0011826
Physical Location & Seq
Reverse (-)
909596 .. 913072
3477 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G001130.1

Sequence Viewer

Length: 3267 bp
ATGGCGAAGATATCAGTGGGTTTGCTAATTACTATAGTTGCTCTTGTTACTGCCCTTATGGTTTATGAATCTGAGGGGCTTAATGATCAGTGTCAATACCTTCTAGAAATAAAGAGTAGAATTGTTGACGGATTTGGCCATCTTAGTAGTTGGAATTCCAGTGATTCAACACCTTGTGGATGGAGGGGAGTGGATTGTAGCGAAGGATTGTATCCGGTGGTCTTGTCTCTTGACTTGAGTTCCATGAATCTTTCGGGTTCTTTATCTCCAAGCATTGGTAGAATGATTCACCTGAATTATCTTGACCTTTCTTTCAATGAAATGTCTGGGAGTATACCTGAAGACATTGGGAATTGTTCCTATTTGCAGGTTCTTAGTTTAAATAACAATAAGTTTGAAGGTCAAATTCCCACAGGATTGGGTAGACTTTCTTATTTAGAAGTCTTGAATGTGTGCAATAATAGAATATCAGGGCCTATCCCAGAAGAAGTTGGAAATCTTTCTTCTCTGTCGCAATTGATTGCATACACTAATAATCTTAGTGGACCACTGCCACACTTCCGGGCAGGGGAGAATTCAATATCAGGAAGCTTACCTTCCGAGATTGGTGAATGTGAAAGCTTGAACTATCTTGGTCTAGCTCAAAATCAGTTAAGCGGAGGAATTCCGAAAGAGATTGGAATGCTCGAGAATTTGAGTGTTTTAATTCTTTGGGAAAATCTGCTTTCTGGTGTCATTCCAAAGGAGCTTGGCAACTGTACAAATTTGTATACTCTGGCCCTCTACAAGAACAAGCTTGAAGGAGAGATACCAAAGGAGCTTGGGAGTCTTGTTTTTCTAGAGAAGCTATATGTGTACAGAAATATGCTGAATGGGACAGTTCCAAGAGAGATTGGTAATCTTTCTCTTGCTACGGAGATTGATTTTTCTGAGAACTTGTTGTCTGGAGAGATACCAGTTGAGCTCAGTAAGATATCGGGTTTGCAATTGCTTTATCTTTTCGAAAACCATCTCACAGGTGTTATTCCAGAGGAGCTCACAAGCTTGACAAACCTGACAAAGCTCGACCTGTCAATAAATTTTCTCACTGGTTCAATTCCAACTGGATTTCAATACATGAATGAGCTGATCATGCTACAGTTGTTTAACAATATGCTGAGTGACATAATTCCTCAAGGACTTGGGGTGTACAGCCCACTTTGGGTGGTTGACTTATCTGACAACTTCCTGACAGGAAGAATTCCCGTACATGTCTGCAGAAATTCTAATCTGATTTTGTTAAACTTGGGGTCTAACAGGCTCACCGGTAATATCCCAACTGGGGTCACAAGCTGCAAGTCATTGGCGCAGCTCCGTCTGGTGGGAAACAACCTTACAGGGACTCTTCCAACCGAAATGTGCAAATTGGTTAACCTTTCTGCAGTTGAGTTGGATCAAAACAAGTTCAGCGGTGCGATTCCTCCAGCAATTGGTAGCTGCAGAACTTTGCAGAGACTGCTACTTGCAGACAACTACTTCACATCTGAGTTGCCAAGAGAGATTGGAGAGCTCTCGCAGTTGGTGATCTTCAATATCTCATCGAATTTACTTTCTGGCAGGATACCACCTGAAATTTTCAATTGTAAAATGCTTCAACGGCTTGATCTGAGTAGTAACAACTTTTCAGATGTCTTGCCAAGCGAGATAGGAGCACTTTCTCAGTTGGAGCTTCTCAAGCTCTCCGATAATCATCTTTCGGGGGATATCCCAGTTGCACTGGGAAATCTCTTGCATTTGACTGAGCTACAAATGGGTGGCAACTCATTCTCTGGGGGTATACCAGCTGAGTTGGGTGCCCTTTCAAGCCTCCAGATTGGATTAAATCTCAGTTATAACAATCTTTCTGGGGAGATACCTCCTCAACTTGGAAATCTTATTTTACTGGAACTGCTTCTACTCGATAACAACCATTTGACTGGTGGAATTCCAGGTTCTTTTGACAACCTGTCAAGGCCTATTCCTTCTCTACCACTCTTTCAGAGTATGGATGTTAGCAGCTTTGTTGGGAATAAAAGACTTTGCGGTGGCCCTCTTGGTGCTTGTGAAACACCATCTTCACTCTCTTTTCCTCCAAATGTGGCAAACAAAAGAACCCGCTTAGGGAAGATTGTTGCTATTGTTGCGGCAGCCATTGGTGGTGTTTCTCTTATCTTAATCGTAATCATTATATATGTGATGAGACGTCCTGTAGACAATGTAGTAACTTCTGCGCAAGAAAAACCACTCTCTTCTCCAGTTTTGGATGTGTACTTTTCGCCAAAGGCAGGATTCACTTTTCAAGACTTGGTTATGGCGACTGAAAACTTTGACGAGAGATTTGCAATTGGAAGGGGAGCTTCTGGAACTGTGTATAAAGCCGTCTTGTTTCACGGTCATACAGTTGCTGTCAAGAAGGTAGTATCCAACAGGGAAGGAAACAATGTGGATAGTAGCTTTCATGCAGAGATTCTAACTTTAGGAAATATCAGGCATCGAAATATTGTGAAGCTGTATGGTTTCTGCTACCACCAGGGTTCCAATCTTCTGCTTTATGAGTACATGGAAAGGGGCAGCTTAGGAGAATTACTTCATGGAAAATCTTGTAATCTTGATTGGCAAACGAGGTTCAATATTGCTCTTGGGGCTGCCCAGGGTCTCTCTTATCTGCATCATGATTGCAAACCTCGGATCTTTCACCGTGATATAAAGTCCAACAACATTCTCCTCGATGAAATGTTTGAAGCTCATGTTGGAGATTTTGGGTTAGCAAAGGTGATTGACATGCCCTATTCGAAGTCAATGTCTGCAGTAGCAGGTTCTTATGGATACATAGCCCCTGAGTATGCATACACTATGAAAGTCACAGAAAAATGTGACATCTACAGCTATGGAGTGGTCCTTCTGGAGTTGCTGACTGGTAAAACACCAGTACAATCATTAGACGAAGGCGGCGACCTGGTAACTTGGGTTAGAACTTACTTTCTCAACAATTCATTGTCATCAGAAGTACTCGATGCTCGATTGAATTTACAAGATGAAGATGAAGCTGAAGCTGCTGCTACTGTCTCTCACATGATTACTGTCTTGAAAATTGCTTTGTTGTGCACAAGCATGTCTCCTTTTGAACGCCCGACCATGAGAGAAGTTGTTTCCATGCTTCTTGGGTCGAATGAGAAAGAAACAGTACAGTTTGCTAATGAGTCCCCAAGTGATGATACTGATACGAATTCGACTTCTAAGATGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

1088

Amino Acids

118.59

Weight (kDa)

5.0

Isoelectric Point (pI)

34.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 27 - 67 2e-11 Leucine rich repeat N-terminal domain
LRR_14 PF23598 74 - 176 1.8e-11 Leucine-rich repeat region
LRR_14 PF23598 201 - 301 2.3e-07 Leucine-rich repeat region
LRR_14 PF23598 533 - 617 1e-07 Leucine-rich repeat region
LRR_8 PF13855 544 - 601 3.1e-06 Leucine rich repeat
Pkinase PF00069 787 - 1057 4.3e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 787 - 1059 1e-43 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011826)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G63930 AT5G63930
fragaria_vesca FvH4_6g01320 FvH4_6g01320
malus_domestica MD04G1235000.v1.1 MD12G1253000.v1.1
prunus_persica Prupe.6G354900_v2.0.a1 Prupe.6G354900_v2.0.a1
pyrus_communis pycom04g20870 pycom12g23210
rosa_chinensis RchiOBHm_Chr3g0448891
rosa_laevigata RLG00000025869
rosa_multiflora Rmu_co8340729.1_g000001 Rmu_sc0003116.1_g000020
rosa_rugosa Rorug02G0617000
rosa_samantha Rh3AG015200 Rh3CG013900 Rh3DG015500
rosa_wichuraiana Rw3G001130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1872
AatII GACGTC 1 cut(s) 2224
Acc16I TGCGCA 1 cut(s) 2250
Acc36I ACCTGC 2 cut(s) 358, 2822
AccB1I GGYRCC 1 cut(s) 1832
AccB7I CCANNNNNTGG 2 cut(s) 275, 1469
AccI GTMKAC 5 cut(s) 334, 424, 770, 1816, 2229
AciI CCGC 6 cut(s) 657, 1449, 2061, 2134, 2162, 2967
AclWI GGATC 2 cut(s) 1440, 2714
AcoI YGGCCR 1 cut(s) 136
AcuI CTGAAG 2 cut(s) 360, 3087
AcyI GRCGYC 1 cut(s) 2221
AdeI CACNNNGTG 1 cut(s) 176
AfiI CCNNNNNNNGG 9 cut(s) 275, 568, 1201, 1321, 1360, 1469, 1904, 2139, 2303
AflIII ACRYGT 1 cut(s) 1249
AgeI ACCGGT 1 cut(s) 1304
AhdI GACNNNNNGTC 1 cut(s) 1984
AjnI CCWGG 4 cut(s) 1966, 2547, 2667, 2973
AjuI GAANNNNNNNTTGG 2 cut(s) 2751, 2783
Alw21I GWGCWC 5 cut(s) 966, 1038, 1551, 1693, 3125
Alw26I GTCTC 6 cut(s) 231, 1486, 2212, 2678, 3088, 3138
Alw44I GTGCAC 1 cut(s) 3121
AlwI GGATC 2 cut(s) 1440, 2714
AlwNI CAGNNNCTG 2 cut(s) 1495, 2423
Ama87I CYCGRG 1 cut(s) 686
AoxI GGCC 5 cut(s) 136, 473, 777, 1991, 2065
ApaLI GTGCAC 1 cut(s) 3121
ApeKI GCWGC 9 cut(s) 1332, 1348, 1476, 2034, 2165, 2589, 2663, 3071, 3074
ArsI GACNNNNNNTTYG 4 cut(s) 2339, 2371, 2804, 2836
AsiGI ACCGGT 1 cut(s) 1304
Asp700I GAANNNNTTC 3 cut(s) 499, 1929, 2604
AspLEI GCGC 2 cut(s) 1348, 2251
AspS9I GGNCC 5 cut(s) 473, 545, 778, 2066, 2914
AsuC2I CCSGG 1 cut(s) 563
AsuHPI GGTGA 6 cut(s) 281, 620, 1294, 1573, 2705, 2803
AsuII TTCGAA 2 cut(s) 1002, 2810
AvaI CYCGRG 1 cut(s) 686
AvaII GGWCC 2 cut(s) 545, 2914
BaeGI GKGCMC 2 cut(s) 1837, 3125
BalI TGGCCA 1 cut(s) 138
BanI GGYRCC 1 cut(s) 1832
BanII GRGCYC 3 cut(s) 966, 1038, 1551
BarI GAAGNNNNNNTAC 2 cut(s) 792, 824
BbsI GAAGAC 1 cut(s) 348
Bbv12I GWGCWC 5 cut(s) 966, 1038, 1551, 1693, 3125
BbvI GCAGC 9 cut(s) 1319, 1360, 1463, 2046, 2177, 2601, 2650, 3058, 3061
BccI CCATC 4 cut(s) 147, 174, 1017, 2098
BceAI ACGGC 2 cut(s) 1652, 2381
BcgI CGANNNNNNTGC 2 cut(s) 2339, 2373
BciT130I CCWGG 4 cut(s) 1968, 2549, 2669, 2975
BciVI GTATCC 4 cut(s) 222, 1593, 2449, 2837
BclI TGATCA 2 cut(s) 85, 1128
BcnI CCSGG 1 cut(s) 563
BcoDI GTCTC 6 cut(s) 231, 1486, 2212, 2678, 3088, 3138
BfaI CTAG 3 cut(s) 104, 638, 839
BfmI CTRYAG 8 cut(s) 33, 1136, 1255, 1419, 1477, 2226, 2823, 2899
BfuAI ACCTGC 2 cut(s) 358, 2822
BfuI GTATCC 4 cut(s) 222, 1593, 2449, 2837
BmcAI AGTACT 1 cut(s) 3027
Bme1390I CCNGG 5 cut(s) 563, 1968, 2549, 2669, 2975
Bme18I GGWCC 2 cut(s) 545, 2914
BmeRI GACNNNNNGTC 1 cut(s) 1984
BmeT110I CYCGRG 1 cut(s) 686
BmgT120I GGNCC 5 cut(s) 473, 545, 778, 2066, 2914
BmiI GGNNCC 2 cut(s) 1834, 2554
BmrFI CCNGG 5 cut(s) 563, 1968, 2549, 2669, 2975
BmrI ACTGGG 3 cut(s) 1329, 1742, 1766
BmsI GCATC 3 cut(s) 2518, 2695, 3022
BmuI ACTGGG 3 cut(s) 1329, 1742, 1766
BpiI GAAGAC 1 cut(s) 348
BpmI CTGGAG 5 cut(s) 966, 1446, 1832, 2256, 2942
Bpu10I CCTNAGC 2 cut(s) 2137, 2593
Bpu14I TTCGAA 2 cut(s) 1002, 2810
BpuEI CTTGAG 3 cut(s) 256, 1158, 1697
BpuMI CCSGG 1 cut(s) 563
BsaBI GATNNNNATC 1 cut(s) 1728
BsaHI GRCGYC 1 cut(s) 2221
BsaI GGTCTC 1 cut(s) 2678
BsaJI CCNNGG 4 cut(s) 2548, 2667, 2668, 2702
BsaWI WCCGGW 2 cut(s) 214, 1304
Bsc4I CCNNNNNNNGG 9 cut(s) 275, 568, 1201, 1321, 1360, 1469, 1904, 2139, 2303
Bse118I RCCGGY 1 cut(s) 1304
Bse8I GATNNNNATC 1 cut(s) 1728
BseBI CCWGG 4 cut(s) 1968, 2549, 2669, 2975
BseDI CCNNGG 4 cut(s) 2548, 2667, 2668, 2702
BseGI GGATG 3 cut(s) 185, 2032, 2287
BseJI GATNNNNATC 1 cut(s) 1728
BseLI CCNNNNNNNGG 9 cut(s) 275, 568, 1201, 1321, 1360, 1469, 1904, 2139, 2303
BseRI GAGGAG 3 cut(s) 1046, 1887, 2732
BseSI GKGCMC 2 cut(s) 1837, 3125
BseXI GCAGC 9 cut(s) 1319, 1360, 1463, 2046, 2177, 2601, 2650, 3058, 3061
BshFI GGCC 5 cut(s) 138, 475, 779, 1993, 2067
BshNI GGYRCC 1 cut(s) 1832
BshTI ACCGGT 1 cut(s) 1304
BsiHKAI GWGCWC 5 cut(s) 966, 1038, 1551, 1693, 3125
BsiHKCI CYCGRG 1 cut(s) 686
BsiSI CCGG 3 cut(s) 215, 562, 1305
BslFI GGGAC 3 cut(s) 889, 1393, 3205
BslI CCNNNNNNNGG 9 cut(s) 275, 568, 1201, 1321, 1360, 1469, 1904, 2139, 2303
BsmAI GTCTC 6 cut(s) 231, 1486, 2212, 2678, 3088, 3138
BsmBI CGTCTC 1 cut(s) 2212
BsmFI GGGAC 3 cut(s) 889, 1393, 3205
BsmI GAATGC 1 cut(s) 687
BsnI GGCC 5 cut(s) 138, 475, 779, 1993, 2067
Bso31I GGTCTC 1 cut(s) 2678
BsoBI CYCGRG 1 cut(s) 686
Bsp119I TTCGAA 2 cut(s) 1002, 2810
Bsp1286I GDGCHC 6 cut(s) 966, 1038, 1551, 1693, 1837, 3125
Bsp1407I TGTACA 3 cut(s) 758, 855, 1188
Bsp143I GATC 6 cut(s) 85, 1128, 1432, 1563, 1642, 2706
BspACI CCGC 6 cut(s) 657, 1449, 2061, 2134, 2162, 2967
BspANI GGCC 5 cut(s) 138, 475, 779, 1993, 2067
BspHI TCATGA 1 cut(s) 2689
BspLI GGNNCC 2 cut(s) 1834, 2554
BspMAI CTGCAG 4 cut(s) 1259, 1423, 1481, 2827
BspMI ACCTGC 2 cut(s) 358, 2822
BspPI GGATC 2 cut(s) 1440, 2714
BspT104I TTCGAA 2 cut(s) 1002, 2810
BspT107I GGYRCC 1 cut(s) 1832
BspTNI GGTCTC 1 cut(s) 2678
BsrFI RCCGGY 1 cut(s) 1304
BsrGI TGTACA 3 cut(s) 758, 855, 1188
BssAI RCCGGY 1 cut(s) 1304
BssECI CCNNGG 4 cut(s) 2548, 2667, 2668, 2702
BssMI GATC 6 cut(s) 85, 1128, 1432, 1563, 1642, 2706
BssNAI GTATAC 3 cut(s) 335, 771, 1817
BssNI GRCGYC 1 cut(s) 2221
Bst1107I GTATAC 3 cut(s) 335, 771, 1817
Bst2UI CCWGG 4 cut(s) 1968, 2549, 2669, 2975
Bst6I CTCTTC 2 cut(s) 1389, 2272
BstACI GRCGYC 1 cut(s) 2221
BstAPI GCANNNNNTGC 1 cut(s) 1495
BstAUI TGTACA 3 cut(s) 758, 855, 1188
BstBI TTCGAA 2 cut(s) 1002, 2810
BstF5I GGATG 3 cut(s) 185, 2032, 2287
BstHHI GCGC 2 cut(s) 1348, 2251
BstKTI GATC 6 cut(s) 88, 1131, 1435, 1566, 1645, 2709
BstMAI GTCTC 6 cut(s) 231, 1486, 2212, 2678, 3088, 3138
BstMBI GATC 6 cut(s) 85, 1128, 1432, 1563, 1642, 2706
BstMWI GCNNNNNNNGC 7 cut(s) 1132, 1495, 1636, 1714, 2159, 2660, 3071
BstNI CCWGG 4 cut(s) 1968, 2549, 2669, 2975
BstNSI RCATGY 3 cut(s) 1253, 2803, 3133
BstSCI CCNGG 5 cut(s) 561, 1966, 2547, 2667, 2973
BstSFI CTRYAG 8 cut(s) 33, 1136, 1255, 1419, 1477, 2226, 2823, 2899
BstSLI GKGCMC 2 cut(s) 1837, 3125
BstV1I GCAGC 9 cut(s) 1319, 1360, 1463, 2046, 2177, 2601, 2650, 3058, 3061
BstV2I GAAGAC 1 cut(s) 348
BstX2I RGATCY 1 cut(s) 2706
BstXI CCANNNNNNTGG 2 cut(s) 418, 1955
BstYI RGATCY 1 cut(s) 2706
BstZ17I GTATAC 3 cut(s) 335, 771, 1817
BsuI GTATCC 4 cut(s) 222, 1593, 2449, 2837
BsuRI GGCC 5 cut(s) 138, 475, 779, 1993, 2067
BtsCI GGATG 3 cut(s) 185, 2032, 2287
BtsI GCAGTG 1 cut(s) 548
BtsIMutI CAGTG 6 cut(s) 21, 95, 166, 548, 1086, 1754
BveI ACCTGC 2 cut(s) 358, 2822
CaiI CAGNNNCTG 2 cut(s) 1495, 2423
CciI TCATGA 1 cut(s) 2689
CfoI GCGC 2 cut(s) 1348, 2251
Cfr10I RCCGGY 1 cut(s) 1304
Cfr13I GGNCC 5 cut(s) 473, 545, 778, 2066, 2914
CsiI ACCWGGT 1 cut(s) 2973
CspAI ACCGGT 1 cut(s) 1304
DpnI GATC 6 cut(s) 87, 1130, 1434, 1565, 1644, 2708
DpnII GATC 6 cut(s) 85, 1128, 1432, 1563, 1642, 2706
DraI TTTAAA 1 cut(s) 381
DraIII CACNNNGTG 1 cut(s) 176
DriI GACNNNNNGTC 1 cut(s) 1984
EaeI YGGCCR 1 cut(s) 136
Eam1104I CTCTTC 2 cut(s) 1389, 2272
Eam1105I GACNNNNNGTC 1 cut(s) 1984
EarI CTCTTC 2 cut(s) 1389, 2272
Ecl136II GAGCTC 3 cut(s) 964, 1036, 1549
Eco147I AGGCCT 1 cut(s) 1993
Eco24I GRGCYC 3 cut(s) 966, 1038, 1551
Eco31I GGTCTC 1 cut(s) 2678
Eco32I GATATC 3 cut(s) 12, 975, 1744
Eco47I GGWCC 2 cut(s) 545, 2914
Eco53kI GAGCTC 3 cut(s) 964, 1036, 1549
Eco57I CTGAAG 2 cut(s) 360, 3087
Eco88I CYCGRG 1 cut(s) 686
EcoICRI GAGCTC 3 cut(s) 964, 1036, 1549
EcoO109I RGGNCCY 1 cut(s) 473
EcoRI GAATTC 6 cut(s) 154, 574, 663, 1239, 1962, 3244
EcoRII CCWGG 4 cut(s) 1966, 2547, 2667, 2973
EcoRV GATATC 3 cut(s) 12, 975, 1744
EcoT22I ATGCAT 1 cut(s) 2866
EcoT38I GRGCYC 3 cut(s) 966, 1038, 1551
Esp3I CGTCTC 1 cut(s) 2212
FalI AAGNNNNNCTT 4 cut(s) 580, 612, 2359, 2391
FaqI GGGAC 3 cut(s) 889, 1393, 3205
FauI CCCGC 1 cut(s) 2141
FbaI TGATCA 2 cut(s) 85, 1128
FblI GTMKAC 5 cut(s) 334, 424, 770, 1816, 2229
FokI GGATG 3 cut(s) 192, 2039, 2294
FriOI GRGCYC 3 cut(s) 966, 1038, 1551
FspBI CTAG 3 cut(s) 104, 638, 839
FspI TGCGCA 1 cut(s) 2250
GlaI GCGC 2 cut(s) 1347, 2250
GsuI CTGGAG 5 cut(s) 966, 1446, 1832, 2256, 2942
HaeIII GGCC 5 cut(s) 138, 475, 779, 1993, 2067
HapII CCGG 3 cut(s) 215, 562, 1305
HhaI GCGC 2 cut(s) 1348, 2251
Hin1I GRCGYC 1 cut(s) 2221
Hin6I GCGC 2 cut(s) 1346, 2249
HinP1I GCGC 2 cut(s) 1346, 2249
HincII GTYRAC 3 cut(s) 127, 1210, 1411
HindII GTYRAC 3 cut(s) 127, 1210, 1411
HindIII AAGCTT 4 cut(s) 589, 619, 794, 1042
HpaI GTTAAC 1 cut(s) 1411
HpaII CCGG 3 cut(s) 215, 562, 1305
HphI GGTGA 6 cut(s) 281, 620, 1294, 1573, 2705, 2803
HpyCH4IV ACGT 1 cut(s) 2221
HpyF10VI GCNNNNNNNGC 7 cut(s) 1132, 1495, 1636, 1714, 2159, 2660, 3071
HpySE526I ACGT 1 cut(s) 2221
Hsp92I GRCGYC 1 cut(s) 2221
HspAI GCGC 2 cut(s) 1346, 2249
Ksp22I TGATCA 2 cut(s) 85, 1128
KspAI GTTAAC 1 cut(s) 1411
Kzo9I GATC 6 cut(s) 85, 1128, 1432, 1563, 1642, 2706
LmnI GCTCC 7 cut(s) 745, 817, 1033, 1356, 1688, 1705, 2372
Lsp1109I GCAGC 9 cut(s) 1319, 1360, 1463, 2046, 2177, 2601, 2650, 3058, 3061
LweI GCATC 3 cut(s) 2518, 2695, 3022
MabI ACCWGGT 1 cut(s) 2973
MaeI CTAG 3 cut(s) 104, 638, 839
MaeII ACGT 1 cut(s) 2221
MaeIII GTNAC 8 cut(s) 46, 1160, 1324, 1652, 2239, 2878, 2891, 2977
MalI GATC 6 cut(s) 87, 1130, 1434, 1565, 1644, 2708
MboI GATC 6 cut(s) 85, 1128, 1432, 1563, 1642, 2706
MfeI CAATTG 5 cut(s) 515, 986, 1467, 1618, 2361
MflI RGATCY 1 cut(s) 2706
MhlI GDGCHC 6 cut(s) 966, 1038, 1551, 1693, 1837, 3125
MlsI TGGCCA 1 cut(s) 138
MluNI TGGCCA 1 cut(s) 138
MlyI GAGTC 3 cut(s) 835, 1375, 3227
MmeI TCCRAC 9 cut(s) 131, 472, 1124, 1410, 1412, 1683, 2466, 2749, 2754
Mox20I TGGCCA 1 cut(s) 138
Mph1103I ATGCAT 1 cut(s) 2866
MroXI GAANNNNTTC 3 cut(s) 499, 1929, 2604
MscI TGGCCA 1 cut(s) 138
MseI TTAA 9 cut(s) 81, 380, 653, 704, 1146, 1280, 1410, 1859, 2192
MslI CAYNNNNRTG 1 cut(s) 3128
Msp20I TGGCCA 1 cut(s) 138
MspA1I CMGCKG 2 cut(s) 1449, 1823
MspI CCGG 3 cut(s) 215, 562, 1305
MspR9I CCNGG 5 cut(s) 563, 1968, 2549, 2669, 2975
MunI CAATTG 5 cut(s) 515, 986, 1467, 1618, 2361
Mva1269I GAATGC 1 cut(s) 687
MvaI CCWGG 4 cut(s) 1968, 2549, 2669, 2975
MwoI GCNNNNNNNGC 7 cut(s) 1132, 1495, 1636, 1714, 2159, 2660, 3071
NciI CCSGG 1 cut(s) 563
NdeII GATC 6 cut(s) 85, 1128, 1432, 1563, 1642, 2706
NlaIV GGNNCC 2 cut(s) 1834, 2554
NmuCI GTSAC 4 cut(s) 1160, 1324, 2878, 2891
NsbI TGCGCA 1 cut(s) 2250
NsiI ATGCAT 1 cut(s) 2866
NspI RCATGY 3 cut(s) 1253, 2803, 3133
NspV TTCGAA 2 cut(s) 1002, 2810
PaeR7I CTCGAG 1 cut(s) 686
PagI TCATGA 1 cut(s) 2689
PasI CCCWGGG 1 cut(s) 2668
PceI AGGCCT 1 cut(s) 1993
PciI ACATGT 1 cut(s) 1249
PcsI WCGNNNNNNNCGW 1 cut(s) 2967
PctI GAATGC 1 cut(s) 687
PdmI GAANNNNTTC 3 cut(s) 499, 1929, 2604
PfeI GAWTC 7 cut(s) 68, 164, 247, 286, 1456, 2307, 2485
PflMI CCANNNNNTGG 2 cut(s) 275, 1469
PinAI ACCGGT 1 cut(s) 1304
PleI GAGTC 3 cut(s) 834, 1375, 3226
PpsI GAGTC 3 cut(s) 834, 1375, 3226
PscI ACATGT 1 cut(s) 1249
PsiI TTATAA 1 cut(s) 1872
Psp124BI GAGCTC 3 cut(s) 966, 1038, 1551
Psp6I CCWGG 4 cut(s) 1966, 2547, 2667, 2973
PspGI CCWGG 4 cut(s) 1966, 2547, 2667, 2973
PspN4I GGNNCC 2 cut(s) 1834, 2554
PspPI GGNCC 5 cut(s) 473, 545, 778, 2066, 2914
PsrI GAACNNNNNNTAC 2 cut(s) 1917, 1949
PstI CTGCAG 4 cut(s) 1259, 1423, 1481, 2827
PstNI CAGNNNCTG 2 cut(s) 1495, 2423
PsuI RGATCY 1 cut(s) 2706
PvuII CAGCTG 1 cut(s) 1823
RseI CAYNNNNRTG 1 cut(s) 3128
SacI GAGCTC 3 cut(s) 966, 1038, 1551
SaqAI TTAA 9 cut(s) 81, 380, 653, 704, 1146, 1280, 1410, 1859, 2192
Sau3AI GATC 6 cut(s) 85, 1128, 1432, 1563, 1642, 2706
Sau96I GGNCC 5 cut(s) 473, 545, 778, 2066, 2914
ScaI AGTACT 1 cut(s) 3027
SchI GAGTC 3 cut(s) 835, 1375, 3227
ScrFI CCNGG 5 cut(s) 563, 1968, 2549, 2669, 2975
SduI GDGCHC 6 cut(s) 966, 1038, 1551, 1693, 1837, 3125
SexAI ACCWGGT 1 cut(s) 2973
SfaNI GCATC 3 cut(s) 2518, 2695, 3022
SfcI CTRYAG 8 cut(s) 33, 1136, 1255, 1419, 1477, 2226, 2823, 2899
Sfr274I CTCGAG 1 cut(s) 686
SfuI TTCGAA 2 cut(s) 1002, 2810
SinI GGWCC 2 cut(s) 545, 2914
SlaI CTCGAG 1 cut(s) 686
SmiMI CAYNNNNRTG 1 cut(s) 3128
SmlI CTYRAG 4 cut(s) 235, 686, 1173, 1712
SmoI CTYRAG 4 cut(s) 235, 686, 1173, 1712
SseBI AGGCCT 1 cut(s) 1993
SsiI CCGC 6 cut(s) 657, 1449, 2061, 2134, 2162, 2967
SspI AATATT 2 cut(s) 2518, 2650
SspMI CTAG 3 cut(s) 104, 638, 839
SstI GAGCTC 3 cut(s) 966, 1038, 1551
StuI AGGCCT 1 cut(s) 1993
StyD4I CCNGG 5 cut(s) 561, 1966, 2547, 2667, 2973
TaiI ACGT 1 cut(s) 2224
TatI WGTACW 9 cut(s) 758, 855, 1188, 2286, 2574, 2947, 3025, 3202, 3261
TauI GCSGC 2 cut(s) 2165, 2970
TfiI GAWTC 7 cut(s) 68, 164, 247, 286, 1456, 2307, 2485
Tru1I TTAA 9 cut(s) 81, 380, 653, 704, 1146, 1280, 1410, 1859, 2192
Tru9I TTAA 9 cut(s) 81, 380, 653, 704, 1146, 1280, 1410, 1859, 2192
TscAI CASTG 6 cut(s) 21, 95, 166, 555, 1093, 1761
TseFI GTSAC 4 cut(s) 1160, 1324, 2878, 2891
TseI GCWGC 9 cut(s) 1332, 1348, 1476, 2034, 2165, 2589, 2663, 3071, 3074
Tsp45I GTSAC 4 cut(s) 1160, 1324, 2878, 2891
TspGWI ACGGA 3 cut(s) 144, 929, 1343
TspRI CASTG 6 cut(s) 21, 95, 166, 555, 1093, 1761
Van91I CCANNNNNTGG 2 cut(s) 275, 1469
VneI GTGCAC 1 cut(s) 3121
VpaK11BI GGWCC 2 cut(s) 545, 2914
XbaI TCTAGA 2 cut(s) 103, 838
XceI RCATGY 3 cut(s) 1253, 2803, 3133
XcmI CCANNNNNNNNNTGG 1 cut(s) 1955
XhoI CTCGAG 1 cut(s) 686
XmiI GTMKAC 5 cut(s) 334, 424, 770, 1816, 2229
XmnI GAANNNNTTC 3 cut(s) 499, 1929, 2604
XspI CTAG 3 cut(s) 104, 638, 839
ZraI GACGTC 1 cut(s) 2222
ZrmI AGTACT 1 cut(s) 3027
Zsp2I ATGCAT 1 cut(s) 2866
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.