pycom12g23210
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0011826
Physical Location & Seq
Forward (+)
23863290 .. 23866250
2961 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g23210.1

Sequence Viewer

Length: 2895 bp
ATGGTTATGGTGAACATGTCATATGGTTTCTTGATTACTATAATTGTCCTTGTTGCTGCCCTTTTGGTTTTCGAATCCGAGGGGCTTAACGATGAGGGTCAATATCTTCTAAAATTCAAGAGCGGATTAGTTGATAGGTTTGATCATCTTGGTAGTTGGAATTCCAATGATTTCACACCTTGTGGATGGAGAGGAGTGAATTGCAGCAGTGGATACAATCCGGTTGTGCTGTCTCTTGACTTGAGCTCAATGAATCTTTCGGGTTATTTGTCTCCGAGCATTGGCGGATTGGGTCACCTGAACTATCTTAATCTTTCTTTCAATGAACTGACTCGGAGTATACCTGAAGACATCGGAAATTGTTCGAGTTTGGAGGTCCTATATTTGAATGACAATAACTTTATAGGTCAAATTCCTAAACCAATAGCTACGCTTTCAAGTTTACGAGTACTGAATCTCTGCAACAATAGATTATCAGGGCCTATCCATGAAGAAATTGGAAATCTTTCATCCCTGTCCCAGTTGATTGCGTACTCTAACAATCTTAGCGGAATTTTGCCGCGCTCTCTTGGAAATCTCAAAAGATTGAGGACTTTCCGGGCAGGGGAGAACTTAATATCCGGACGCTTGCCTACAGAGATAGGTGAATGTGAGAGCTTGGAGTTTCTTGGTCTAGCGGAAAATCAGTTAAGTGAAGAAATGCCTAAGGAGCTTGGAATGCTTGAGAATTTGGGAGCTTTGTTTCTTTGGGGTAACCAGCTTTCCGGGGTTATTCCAAAGGAGCTTGGCAACTGTACAAAGTTGGAAATTCTTGCCCTGTATGAGAACAAGCTTGTGGGAGAGATTCCAAGGGAGCTCGGAAACATTGCTTATCTGAAGAAGTTGTATCTTTACAGAAATATGTTGAATGGGACAGTTCCAAGAGAGATTGGCAATCTTTCTCTAGTAGCACAGATTGATCGATCGGAGAACTTATTATCGGGAGAGATACCGGTTGAGCTCAGTAAGATAGCGGGATTGCAATTGCTTTATCTTTTCAAGAACCAATTCACAGGTGTTATTCCAGATGAGTTCACAAGTCTTACAAACCTGACTAAGCTAGACCTCTCTATCAATTTTCTCACCGGTTCAATTCCTACCAGAATCCAGTACTTGACTGAACTGATCATGCTGCAATTGTTCCACAATTTGCTGAGTGGCGTAATCCCTCAAGGCCTTGGAGTGTATAGCGCGCTTTGGGTGGTTGACTTATCCGAAAACTTCTTAACGGGGGGAGTTCCTCATCATCTCTGCAGAAATTCTAACATGATTTTGTTAAATTTGGGGTCTAACAGGCTCACTGGGAATATACCAAATGGCATCACAAGCTGCAAGTCATTAGTGCAGCTTCGTCTAGTTGGAAACAACCTCACGGGTACTTTTCCATCCGAAATGTGCAAACTGGTTAACCTTTCTACAATGGAGTTGGATCAGAACAAGTTCAGGGGAGCCATTCCTCCAGAAATTGGCAACTGTAGAACTTTGCAAAGGCTGCACCTTTCGGGCAACTACTTCACATCGGAGTTGCCTAGAGAGATTGGTAGCCTCCCACAGTTGGTGACCTTTAATGTCTCATCAAATTCTCTTTCTGGAAGGATACCGCCTGAAATTTTCAATTGTAAAACACTTCAACGGCTTGATCTTAGCAATAACAACTTGTCAGATGCATTGCCAAGTGAGATAGGAACACTTGCTCAGTTGGAGCTTCTCAAGCTTTCTGAAAACAATCTTTCCGGAAACATACCAGTTGCGCTGGGAAACCTTGTACGTTTGACCGAGCTACAAATGGGTGGCAACTCATTCTCTGGCAGCATCCCAGCGGAGTTGGGTTCCCTTTCGAGCCTGCAGATTGCATTAAATCTCAGTTTTAACAACCTTTCCGGGCAGATACCTCCTCAGCTTGGAAATCTTATCTTACTGGAATTCCTGCTACTCAATAACAACAATTTGACAGGTGTAATTCCAGGTGTTTTCCAGAATCTTTTGAGTCTACTTGTCTGCAACCTGTCGTACAATGCCTTGGCAGGGCCTATCCCTCATTTACCATTCTTTCAGAATATGAATGCTGACAGCTTTGTTGGGAATAAAGGACTCTGCGGCGGGCCTCTTGGTGATTGCGCAACATTACCATCTTCACTCTCTTTTCCTCCAGATTTGGCGAAGAAACGCCCCCACTTGGGTAAAATCATCGCTATCATCGCAGCAGCTATTGGTGGTGTTTCTCTCATTTTGATTGCAATCCTTGTTTATGTCATGAGACGTCCCACGAGCGCTGCAGGGTTCACTTTCCAAGACTTGGTTGCGGCGACTGAAAATATTGATGAGAGCTTTGCAATTGGAAGAGGAGCTTGTGGAACCGTGTACAAAGCAGTCTTACCGACGGGTCATACAGTTGCTGTTAAGAAAGTAGTATCCAGCAGGGAGGGGAACAATGTGGATAACAGTTTTCATGCTGAAATTCTTACTTTAGGAAAAATCAGGCATCGAAATATTGTAAAGCTATACGGTTTCTGCTACTACCAGGGTTGCAATCTTCTGCTCTATGAGTACATGGCGAGAGGCAGCTTGGGAGAATTGCTTCATGGAACATCTTGTAGTCTTGATTGGACAACGAGATTCATGATTGCCCTTGGGGCTGCTCAGGGTCTCTCTTACTTGCATCATGATTCCAAACCGATAATCTTTCACCGTGATATAAAGTCCAACGACATTCTTCTTGACGACAAGTTTGAATCTCGTGTTGGGGATTTCGGGTTGGCAAAGGTGATGGACATGCCACACTCTACGACTCTGTCTGTAGTTGCAGGTTCTTATGGATACATTACTCCTGGTAAGCTATCTTTTAACTATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

965

Amino Acids

104.96

Weight (kDa)

6.06

Isoelectric Point (pI)

30.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 29 - 69 6.1e-12 Leucine rich repeat N-terminal domain
LRR_14 PF23598 79 - 200 9.3e-13 Leucine-rich repeat region
LRR_4 PF12799 100 - 139 4.6e-07 Leucine Rich repeats (2 copies)
LRR_14 PF23598 189 - 297 3.4e-08 Leucine-rich repeat region
LRR_8 PF13855 339 - 398 6.5e-06 Leucine rich repeat
LRR_8 PF13855 530 - 590 1.4e-06 Leucine rich repeat
LRR_14 PF23598 545 - 636 2.4e-09 Leucine-rich repeat region
LRR_8 PF13855 555 - 613 3.3e-07 Leucine rich repeat
Pkinase PF00069 791 - 958 1.6e-30 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 791 - 937 1.2e-26 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011826)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G63930 AT5G63930
fragaria_vesca FvH4_6g01320 FvH4_6g01320
malus_domestica MD04G1235000.v1.1 MD12G1253000.v1.1
prunus_persica Prupe.6G354900_v2.0.a1 Prupe.6G354900_v2.0.a1
pyrus_communis pycom04g20870 pycom12g23210
rosa_chinensis RchiOBHm_Chr3g0448891
rosa_laevigata RLG00000025869
rosa_multiflora Rmu_co8340729.1_g000001 Rmu_sc0003116.1_g000020
rosa_rugosa Rorug02G0617000
rosa_samantha Rh3AG015200 Rh3CG013900 Rh3DG015500
rosa_wichuraiana Rw3G001130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2302
Acc16I TGCGCA 1 cut(s) 2158
Acc36I ACCTGC 1 cut(s) 2834
AccB7I CCANNNNNTGG 2 cut(s) 1505, 2335
AccBSI CCGCTC 1 cut(s) 123
AccI GTMKAC 2 cut(s) 340, 2029
AccII CGCG 2 cut(s) 562, 1232
AccIII TCCGGA 2 cut(s) 620, 1772
AclWI GGATC 1 cut(s) 1476
AcuI CTGAAG 2 cut(s) 366, 896
AcyI GRCGYC 1 cut(s) 2299
AdeI CACNNNGTG 1 cut(s) 182
AfaI GTAC 9 cut(s) 450, 533, 796, 1151, 1417, 1806, 2051, 2402, 2588
AfeI AGCGCT 1 cut(s) 2311
AfiI CCNNNNNNNGG 9 cut(s) 281, 604, 1505, 1594, 1940, 2064, 2215, 2216, 2335
AflIII ACRYGT 1 cut(s) 15
AgeI ACCGGT 2 cut(s) 991, 1124
AjnI CCWGG 3 cut(s) 2002, 2559, 2866
AjuI GAANNNNNNNTTGG 2 cut(s) 2763, 2795
AloI GAACNNNNNNTCC 2 cut(s) 602, 634
Alw21I GWGCWC 3 cut(s) 248, 858, 1002
Alw26I GTCTC 5 cut(s) 237, 276, 1615, 2290, 2690
AlwI GGATC 1 cut(s) 1476
AlwNI CAGNNNCTG 1 cut(s) 2435
Aor13HI TCCGGA 2 cut(s) 620, 1772
Aor51HI AGCGCT 1 cut(s) 2311
AoxI GGCC 4 cut(s) 479, 1213, 2066, 2141
ArsI GACNNNNNNTTYG 2 cut(s) 2750, 2782
AsiGI ACCGGT 2 cut(s) 991, 1124
Asp700I GAANNNNTTC 5 cut(s) 361, 505, 1046, 1478, 2616
AspLEI GCGC 6 cut(s) 564, 1232, 1234, 1792, 2159, 2312
AspS9I GGNCC 4 cut(s) 376, 479, 2066, 2141
AsuC2I CCSGG 3 cut(s) 599, 766, 1921
AsuHPI GGTGA 8 cut(s) 22, 287, 656, 1114, 1609, 2162, 2717, 2815
AsuII TTCGAA 1 cut(s) 72
AvaII GGWCC 1 cut(s) 376
AxyI CCTNAGG 1 cut(s) 705
BanII GRGCYC 3 cut(s) 248, 858, 1002
BauI CACGAG 2 cut(s) 2305, 2775
BbsI GAAGAC 1 cut(s) 354
Bbv12I GWGCWC 3 cut(s) 248, 858, 1002
BbvCI CCTCAGC 1 cut(s) 1935
BccI CCATC 4 cut(s) 180, 1432, 2176, 2800
BceAI ACGGC 1 cut(s) 1688
BciT130I CCWGG 3 cut(s) 2004, 2561, 2868
BciVI GTATCC 4 cut(s) 206, 1629, 2461, 2849
BclI TGATCA 2 cut(s) 142, 1164
BcnI CCSGG 3 cut(s) 599, 766, 1921
BcoDI GTCTC 5 cut(s) 237, 276, 1615, 2290, 2690
BfaI CTAG 5 cut(s) 674, 944, 1100, 1394, 1569
BfmI CTRYAG 6 cut(s) 633, 1291, 1513, 1883, 2313, 2835
BfoI RGCGCY 1 cut(s) 2313
BfuAI ACCTGC 1 cut(s) 2834
BfuI GTATCC 4 cut(s) 206, 1629, 2461, 2849
BglI GCCNNNNNGGC 1 cut(s) 2672
BmcAI AGTACT 2 cut(s) 450, 1151
Bme1390I CCNGG 6 cut(s) 599, 766, 1921, 2004, 2561, 2868
Bme18I GGWCC 1 cut(s) 376
BmgT120I GGNCC 4 cut(s) 376, 479, 2066, 2141
BmiI GGNNCC 3 cut(s) 1489, 1870, 2395
BmrFI CCNGG 6 cut(s) 599, 766, 1921, 2004, 2561, 2868
BmrI ACTGGG 2 cut(s) 514, 1350
BmsI GCATC 5 cut(s) 1368, 1693, 1860, 2530, 2707
BmuI ACTGGG 2 cut(s) 514, 1350
BpiI GAAGAC 1 cut(s) 354
BpmI CTGGAG 2 cut(s) 1482, 2172
Bpu10I CCTNAGC 2 cut(s) 1935, 2679
Bpu14I TTCGAA 1 cut(s) 72
BpuEI CTTGAG 4 cut(s) 262, 743, 1194, 1733
BpuMI CCSGG 3 cut(s) 599, 766, 1921
Bsa29I ATCGAT 1 cut(s) 961
BsaBI GATNNNNATC 1 cut(s) 2276
BsaHI GRCGYC 1 cut(s) 2299
BsaI GGTCTC 1 cut(s) 2690
BsaJI CCNNGG 7 cut(s) 78, 765, 848, 1216, 2058, 2560, 2668
BsaWI WCCGGW 5 cut(s) 220, 620, 991, 1124, 1772
Bsc4I CCNNNNNNNGG 9 cut(s) 281, 604, 1505, 1594, 1940, 2064, 2215, 2216, 2335
Bse118I RCCGGY 2 cut(s) 991, 1124
Bse1I ACTGG 6 cut(s) 520, 1147, 1345, 1446, 1784, 1962
Bse21I CCTNAGG 1 cut(s) 705
Bse3DI GCAATG 2 cut(s) 864, 1706
Bse8I GATNNNNATC 1 cut(s) 2276
BseAI TCCGGA 2 cut(s) 620, 1772
BseBI CCWGG 3 cut(s) 2004, 2561, 2868
BseCI ATCGAT 1 cut(s) 961
BseDI CCNNGG 7 cut(s) 78, 765, 848, 1216, 2058, 2560, 2668
BseGI GGATG 4 cut(s) 191, 509, 1424, 1851
BseJI GATNNNNATC 1 cut(s) 2276
BseLI CCNNNNNNNGG 9 cut(s) 281, 604, 1505, 1594, 1940, 2064, 2215, 2216, 2335
BseMI GCAATG 2 cut(s) 864, 1706
BseMII CTCAG 6 cut(s) 1015, 1184, 1748, 1915, 1949, 2693
BseNI ACTGG 6 cut(s) 520, 1147, 1345, 1446, 1784, 1962
BsePI GCGCGC 1 cut(s) 1230
BseRI GAGGAG 3 cut(s) 207, 1923, 2397
BseYI CCCAGC 2 cut(s) 1792, 1855
BsgI GTGCAG 2 cut(s) 1403, 1517
Bsh1236I CGCG 2 cut(s) 562, 1232
Bsh1285I CGRYCG 1 cut(s) 965
BshFI GGCC 4 cut(s) 481, 1215, 2068, 2143
BshTI ACCGGT 2 cut(s) 991, 1124
BshVI ATCGAT 1 cut(s) 961
BsiEI CGRYCG 1 cut(s) 965
BsiHKAI GWGCWC 3 cut(s) 248, 858, 1002
BsiSI CCGG 8 cut(s) 221, 598, 621, 765, 992, 1125, 1773, 1920
BslFI GGGAC 3 cut(s) 502, 925, 2286
BslI CCNNNNNNNGG 9 cut(s) 281, 604, 1505, 1594, 1940, 2064, 2215, 2216, 2335
BsmAI GTCTC 5 cut(s) 237, 276, 1615, 2290, 2690
BsmBI CGTCTC 1 cut(s) 2290
BsmFI GGGAC 3 cut(s) 502, 925, 2286
BsmI GAATGC 2 cut(s) 723, 2107
BsnI GGCC 4 cut(s) 481, 1215, 2068, 2143
Bso31I GGTCTC 1 cut(s) 2690
Bsp119I TTCGAA 1 cut(s) 72
Bsp1286I GDGCHC 3 cut(s) 248, 858, 1002
Bsp13I TCCGGA 2 cut(s) 620, 1772
Bsp1407I TGTACA 2 cut(s) 794, 2400
Bsp143I GATC 6 cut(s) 142, 958, 962, 1164, 1468, 1678
BspANI GGCC 4 cut(s) 481, 1215, 2068, 2143
BspCNI CTCAG 6 cut(s) 1014, 1185, 1747, 1914, 1948, 2692
BspDI ATCGAT 1 cut(s) 961
BspEI TCCGGA 2 cut(s) 620, 1772
BspFNI CGCG 2 cut(s) 562, 1232
BspHI TCATGA 3 cut(s) 2292, 2658, 2701
BspLI GGNNCC 3 cut(s) 1489, 1870, 2395
BspMAI CTGCAG 3 cut(s) 1295, 1887, 2317
BspMI ACCTGC 1 cut(s) 2834
BspPI GGATC 1 cut(s) 1476
BspT104I TTCGAA 1 cut(s) 72
BspTNI GGTCTC 1 cut(s) 2690
BsrBI CCGCTC 1 cut(s) 123
BsrDI GCAATG 2 cut(s) 864, 1706
BsrFI RCCGGY 2 cut(s) 991, 1124
BsrGI TGTACA 2 cut(s) 794, 2400
BsrI ACTGG 6 cut(s) 520, 1147, 1345, 1446, 1784, 1962
BssAI RCCGGY 2 cut(s) 991, 1124
BssECI CCNNGG 7 cut(s) 78, 765, 848, 1216, 2058, 2560, 2668
BssHII GCGCGC 1 cut(s) 1230
BssMI GATC 6 cut(s) 142, 958, 962, 1164, 1468, 1678
BssNAI GTATAC 1 cut(s) 341
BssNI GRCGYC 1 cut(s) 2299
BssSI CACGAG 2 cut(s) 2305, 2775
BssT1I CCWWGG 4 cut(s) 848, 1216, 2058, 2668
Bst1107I GTATAC 1 cut(s) 341
Bst2BI CACGAG 2 cut(s) 2305, 2775
Bst2UI CCWGG 3 cut(s) 2004, 2561, 2868
Bst4CI ACNGT 9 cut(s) 794, 916, 1514, 1593, 2398, 2431, 2483, 2546, 2729
Bst6I CTCTTC 1 cut(s) 2374
BstACI GRCGYC 1 cut(s) 2299
BstAPI GCANNNNNTGC 1 cut(s) 1531
BstAUI TGTACA 2 cut(s) 794, 2400
BstBI TTCGAA 1 cut(s) 72
BstC8I GCNNGC 4 cut(s) 629, 1232, 1883, 2141
BstEII GGTNACC 3 cut(s) 293, 752, 1597
BstF5I GGATG 4 cut(s) 191, 509, 1424, 1851
BstFNI CGCG 2 cut(s) 562, 1232
BstH2I RGCGCY 1 cut(s) 2313
BstHHI GCGC 6 cut(s) 564, 1232, 1234, 1792, 2159, 2312
BstKTI GATC 6 cut(s) 145, 961, 965, 1167, 1471, 1681
BstMAI GTCTC 5 cut(s) 237, 276, 1615, 2290, 2690
BstMBI GATC 6 cut(s) 142, 958, 962, 1164, 1468, 1678
BstMCI CGRYCG 1 cut(s) 965
BstMWI GCNNNNNNNGC 7 cut(s) 709, 718, 1365, 1531, 1750, 2237, 2672
BstNI CCWGG 3 cut(s) 2004, 2561, 2868
BstNSI RCATGY 2 cut(s) 19, 2815
BstPI GGTNACC 3 cut(s) 293, 752, 1597
BstSCI CCNGG 6 cut(s) 597, 764, 1919, 2002, 2559, 2866
BstSFI CTRYAG 6 cut(s) 633, 1291, 1513, 1883, 2313, 2835
BstUI CGCG 2 cut(s) 562, 1232
BstV2I GAAGAC 1 cut(s) 354
BstZ17I GTATAC 1 cut(s) 341
Bsu15I ATCGAT 1 cut(s) 961
Bsu36I CCTNAGG 1 cut(s) 705
BsuI GTATCC 4 cut(s) 206, 1629, 2461, 2849
BsuRI GGCC 4 cut(s) 481, 1215, 2068, 2143
BsuTUI ATCGAT 1 cut(s) 961
BtgZI GCGATG 2 cut(s) 2212, 2221
BtsCI GGATG 4 cut(s) 191, 509, 1424, 1851
BtsI GCAGTG 1 cut(s) 214
BtsIMutI CAGTG 2 cut(s) 214, 1338
BveI ACCTGC 1 cut(s) 2834
Cac8I GCNNGC 4 cut(s) 629, 1232, 1883, 2141
CaiI CAGNNNCTG 1 cut(s) 2435
CciI TCATGA 3 cut(s) 2292, 2658, 2701
CfoI GCGC 6 cut(s) 564, 1232, 1234, 1792, 2159, 2312
Cfr10I RCCGGY 2 cut(s) 991, 1124
Cfr13I GGNCC 4 cut(s) 376, 479, 2066, 2141
ClaI ATCGAT 1 cut(s) 961
CseI GACGC 1 cut(s) 633
Csp6I GTAC 9 cut(s) 449, 532, 795, 1150, 1416, 1805, 2050, 2401, 2587
CspAI ACCGGT 2 cut(s) 991, 1124
CviQI GTAC 9 cut(s) 449, 532, 795, 1150, 1416, 1805, 2050, 2401, 2587
DpnI GATC 6 cut(s) 144, 960, 964, 1166, 1470, 1680
DpnII GATC 6 cut(s) 142, 958, 962, 1164, 1468, 1678
DraIII CACNNNGTG 1 cut(s) 182
Eam1104I CTCTTC 1 cut(s) 2374
EarI CTCTTC 1 cut(s) 2374
EciI GGCGGA 1 cut(s) 300
Ecl136II GAGCTC 3 cut(s) 246, 856, 1000
Eco130I CCWWGG 4 cut(s) 848, 1216, 2058, 2668
Eco147I AGGCCT 1 cut(s) 1215
Eco24I GRGCYC 3 cut(s) 248, 858, 1002
Eco31I GGTCTC 1 cut(s) 2690
Eco47I GGWCC 1 cut(s) 376
Eco47III AGCGCT 1 cut(s) 2311
Eco53kI GAGCTC 3 cut(s) 246, 856, 1000
Eco57I CTGAAG 2 cut(s) 366, 896
Eco81I CCTNAGG 1 cut(s) 705
Eco91I GGTNACC 3 cut(s) 293, 752, 1597
EcoICRI GAGCTC 3 cut(s) 246, 856, 1000
EcoO109I RGGNCCY 3 cut(s) 376, 479, 2066
EcoO65I GGTNACC 3 cut(s) 293, 752, 1597
EcoRI GAATTC 2 cut(s) 160, 1961
EcoRII CCWGG 3 cut(s) 2002, 2559, 2866
EcoT14I CCWWGG 4 cut(s) 848, 1216, 2058, 2668
EcoT22I ATGCAT 1 cut(s) 1708
EcoT38I GRGCYC 3 cut(s) 248, 858, 1002
ErhI CCWWGG 4 cut(s) 848, 1216, 2058, 2668
Esp3I CGTCTC 1 cut(s) 2290
FalI AAGNNNNNCTT 2 cut(s) 2371, 2403
FaqI GGGAC 3 cut(s) 502, 925, 2286
FauI CCCGC 2 cut(s) 1006, 2132
FauNDI CATATG 1 cut(s) 22
FbaI TGATCA 2 cut(s) 142, 1164
FblI GTMKAC 2 cut(s) 340, 2029
FokI GGATG 4 cut(s) 198, 496, 1411, 1838
FriOI GRGCYC 3 cut(s) 248, 858, 1002
FspBI CTAG 5 cut(s) 674, 944, 1100, 1394, 1569
FspI TGCGCA 1 cut(s) 2158
GlaI GCGC 6 cut(s) 563, 1231, 1233, 1791, 2158, 2311
GsaI CCCAGC 2 cut(s) 1796, 1859
GsuI CTGGAG 2 cut(s) 1482, 2172
HaeII RGCGCY 1 cut(s) 2313
HaeIII GGCC 4 cut(s) 481, 1215, 2068, 2143
HapII CCGG 8 cut(s) 221, 598, 621, 765, 992, 1125, 1773, 1920
HgaI GACGC 1 cut(s) 633
HhaI GCGC 6 cut(s) 564, 1232, 1234, 1792, 2159, 2312
Hin1I GRCGYC 1 cut(s) 2299
Hin6I GCGC 6 cut(s) 562, 1230, 1232, 1790, 2157, 2310
HinP1I GCGC 6 cut(s) 562, 1230, 1232, 1790, 2157, 2310
HincII GTYRAC 2 cut(s) 1246, 1447
HindII GTYRAC 2 cut(s) 1246, 1447
HindIII AAGCTT 2 cut(s) 830, 1751
HpaI GTTAAC 1 cut(s) 1447
HpaII CCGG 8 cut(s) 221, 598, 621, 765, 992, 1125, 1773, 1920
HphI GGTGA 8 cut(s) 22, 287, 656, 1114, 1609, 2162, 2717, 2815
Hpy166II GTNNAC 9 cut(s) 13, 341, 443, 1074, 1246, 1447, 2030, 2322, 2401
Hpy8I GTNNAC 9 cut(s) 13, 341, 443, 1074, 1246, 1447, 2030, 2322, 2401
Hpy99I CGWCG 1 cut(s) 2422
HpyAV CCTTC 1 cut(s) 1626
HpyCH4III ACNGT 9 cut(s) 794, 916, 1514, 1593, 2398, 2431, 2483, 2546, 2729
HpyCH4IV ACGT 2 cut(s) 1807, 2299
HpyF10VI GCNNNNNNNGC 7 cut(s) 709, 718, 1365, 1531, 1750, 2237, 2672
HpySE526I ACGT 2 cut(s) 1807, 2299
Hsp92I GRCGYC 1 cut(s) 2299
HspAI GCGC 6 cut(s) 562, 1230, 1232, 1790, 2157, 2310
Kpn2I TCCGGA 2 cut(s) 620, 1772
Ksp22I TGATCA 2 cut(s) 142, 1164
KspAI GTTAAC 1 cut(s) 1447
Kzo9I GATC 6 cut(s) 142, 958, 962, 1164, 1468, 1678
LmnI GCTCC 7 cut(s) 709, 734, 781, 853, 1487, 1741, 2384
LweI GCATC 5 cut(s) 1368, 1693, 1860, 2530, 2707
MaeI CTAG 5 cut(s) 674, 944, 1100, 1394, 1569
MaeII ACGT 2 cut(s) 1807, 2299
MaeIII GTNAC 3 cut(s) 293, 752, 1597
MalI GATC 6 cut(s) 144, 960, 964, 1166, 1470, 1680
MbiI CCGCTC 1 cut(s) 123
MboI GATC 6 cut(s) 142, 958, 962, 1164, 1468, 1678
MfeI CAATTG 4 cut(s) 1022, 1175, 1654, 2373
MhlI GDGCHC 3 cut(s) 248, 858, 1002
MlyI GAGTC 4 cut(s) 325, 2035, 2124, 2821
MmeI TCCRAC 6 cut(s) 137, 783, 1378, 1446, 1719, 2766
Mph1103I ATGCAT 1 cut(s) 1708
MroI TCCGGA 2 cut(s) 620, 1772
MroXI GAANNNNTTC 5 cut(s) 361, 505, 1046, 1478, 2616
MspA1I CMGCKG 1 cut(s) 1859
MspI CCGG 8 cut(s) 221, 598, 621, 765, 992, 1125, 1773, 1920
MspR9I CCNGG 6 cut(s) 599, 766, 1921, 2004, 2561, 2868
MunI CAATTG 4 cut(s) 1022, 1175, 1654, 2373
Mva1269I GAATGC 2 cut(s) 723, 2107
MvaI CCWGG 3 cut(s) 2004, 2561, 2868
MvnI CGCG 2 cut(s) 562, 1232
MwoI GCNNNNNNNGC 7 cut(s) 709, 718, 1365, 1531, 1750, 2237, 2672
NciI CCSGG 3 cut(s) 599, 766, 1921
NdeI CATATG 1 cut(s) 22
NdeII GATC 6 cut(s) 142, 958, 962, 1164, 1468, 1678
NlaIV GGNNCC 3 cut(s) 1489, 1870, 2395
NmuCI GTSAC 2 cut(s) 293, 1597
NsbI TGCGCA 1 cut(s) 2158
NsiI ATGCAT 1 cut(s) 1708
NspI RCATGY 2 cut(s) 19, 2815
NspV TTCGAA 1 cut(s) 72
PagI TCATGA 3 cut(s) 2292, 2658, 2701
PauI GCGCGC 1 cut(s) 1230
PceI AGGCCT 1 cut(s) 1215
PciI ACATGT 1 cut(s) 15
PctI GAATGC 2 cut(s) 723, 2107
PdmI GAANNNNTTC 5 cut(s) 361, 505, 1046, 1478, 2616
PfeI GAWTC 9 cut(s) 74, 253, 454, 844, 1143, 2017, 2655, 2705, 2771
PflFI GACNNNGTC 1 cut(s) 2830
PflMI CCANNNNNTGG 2 cut(s) 1505, 2335
PinAI ACCGGT 2 cut(s) 991, 1124
Ple19I CGATCG 1 cut(s) 965
PleI GAGTC 4 cut(s) 325, 2034, 2124, 2821
PpsI GAGTC 4 cut(s) 325, 2034, 2124, 2821
PpuMI RGGWCCY 1 cut(s) 376
PscI ACATGT 1 cut(s) 15
Psp124BI GAGCTC 3 cut(s) 248, 858, 1002
Psp5II RGGWCCY 1 cut(s) 376
Psp6I CCWGG 3 cut(s) 2002, 2559, 2866
PspEI GGTNACC 3 cut(s) 293, 752, 1597
PspFI CCCAGC 2 cut(s) 1792, 1855
PspGI CCWGG 3 cut(s) 2002, 2559, 2866
PspN4I GGNNCC 3 cut(s) 1489, 1870, 2395
PspPI GGNCC 4 cut(s) 376, 479, 2066, 2141
PspPPI RGGWCCY 1 cut(s) 376
PstI CTGCAG 3 cut(s) 1295, 1887, 2317
PstNI CAGNNNCTG 1 cut(s) 2435
PsyI GACNNNGTC 1 cut(s) 2830
PteI GCGCGC 1 cut(s) 1230
PvuI CGATCG 1 cut(s) 965
RsaI GTAC 9 cut(s) 450, 533, 796, 1151, 1417, 1806, 2051, 2402, 2588
RsaNI GTAC 9 cut(s) 449, 532, 795, 1150, 1416, 1805, 2050, 2401, 2587
SacI GAGCTC 3 cut(s) 248, 858, 1002
Sau3AI GATC 6 cut(s) 142, 958, 962, 1164, 1468, 1678
Sau96I GGNCC 4 cut(s) 376, 479, 2066, 2141
ScaI AGTACT 2 cut(s) 450, 1151
SchI GAGTC 4 cut(s) 325, 2035, 2124, 2821
ScrFI CCNGG 6 cut(s) 599, 766, 1921, 2004, 2561, 2868
SduI GDGCHC 3 cut(s) 248, 858, 1002
SfaNI GCATC 5 cut(s) 1368, 1693, 1860, 2530, 2707
SfcI CTRYAG 6 cut(s) 633, 1291, 1513, 1883, 2313, 2835
SfuI TTCGAA 1 cut(s) 72
SinI GGWCC 1 cut(s) 376
SmlI CTYRAG 4 cut(s) 241, 722, 1209, 1748
SmoI CTYRAG 4 cut(s) 241, 722, 1209, 1748
SseBI AGGCCT 1 cut(s) 1215
SspI AATATT 2 cut(s) 2356, 2530
SspMI CTAG 5 cut(s) 674, 944, 1100, 1394, 1569
SstI GAGCTC 3 cut(s) 248, 858, 1002
StuI AGGCCT 1 cut(s) 1215
StyD4I CCNGG 6 cut(s) 597, 764, 1919, 2002, 2559, 2866
StyI CCWWGG 4 cut(s) 848, 1216, 2058, 2668
TaaI ACNGT 9 cut(s) 794, 916, 1514, 1593, 2398, 2431, 2483, 2546, 2729
TaiI ACGT 2 cut(s) 1810, 2302
TaqI TCGA 5 cut(s) 72, 365, 961, 1877, 2524
TaqII GACCGA 1 cut(s) 1829
TatI WGTACW 5 cut(s) 448, 794, 1149, 2400, 2586
TauI GCSGC 3 cut(s) 562, 2139, 2345
TfiI GAWTC 9 cut(s) 74, 253, 454, 844, 1143, 2017, 2655, 2705, 2771
TscAI CASTG 2 cut(s) 214, 1345
TseFI GTSAC 2 cut(s) 293, 1597
Tsp45I GTSAC 2 cut(s) 293, 1597
TspDTI ATGAA 8 cut(s) 266, 339, 498, 504, 2114, 2477, 2609, 2647
TspRI CASTG 2 cut(s) 214, 1345
Tth111I GACNNNGTC 1 cut(s) 2830
Van91I CCANNNNNTGG 2 cut(s) 1505, 2335
VpaK11BI GGWCC 1 cut(s) 376
XceI RCATGY 2 cut(s) 19, 2815
XcmI CCANNNNNNNNNTGG 1 cut(s) 494
XmiI GTMKAC 2 cut(s) 340, 2029
XmnI GAANNNNTTC 5 cut(s) 361, 505, 1046, 1478, 2616
XspI CTAG 5 cut(s) 674, 944, 1100, 1394, 1569
ZraI GACGTC 1 cut(s) 2300
ZrmI AGTACT 2 cut(s) 450, 1151
Zsp2I ATGCAT 1 cut(s) 1708
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.