RLG00000025869
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0011826
Physical Location & Seq
Forward (+)
48825914 .. 48829361
3448 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025869

Sequence Viewer

Length: 3303 bp
ATGGCGAAGATATCAGTGGGTTTGCTAATTACTATAGTTGCTCTTGTTACTGCCCTTATGGTTTATGAATCTGAGGAGCTTAATGATCAGGGTCAATACCTTCTAGAAATAAAGAGTAGAATTGTTGACGGATTTGGCCATCTTAGTAGTTGGAATTCCAGTGATTCAACACCCTGTGGATGGAGGGGAGTGGATTGTAGCGAAGGATTGTATCCGGCGGTCTTGTCTCTTGACTTGAGTTCCATGAATCTTTCGGGTTCTTTATCTCCAAGCATTGGTAGAATGATTCACCTGAATTATCTTGACCTTTCTTTCAATGAAATGTCTGGGAGTATACCTGAAGACATTGGGAATTGTTCCTATTTGCAGGTTCTTAGTTTAAATAACAATAAGTTTGAAGGTCAAATTCCCACAGGATTGGGTAGACTTTCTTATTTAGAAGTCTTGAATGTGTGCAATAATAGAATATCAGGGCCTTTCCCAGAAGAACTTGGAAATCTTTCTTCTCTGTCGCAATTGATTGCATACACTAATAATCTTAGTGGACCACTGCCACGTTCTATTGGCAACCTCAAAAGATTGAAGATTTTCCGGGCAGGGGAGAATTCAATATCAGGAAGCTTACCTTCCGAGATTGGTGAATGTGAAAGCTTGAACTATCTTGGTCTAGCTCAAAATCAGTTAAGCGGAGAAATTCCGAAAGAGATTGGAATGCTCGAGAATTTGAGTGTTTTAATTCTTTGGGAAAATCTGCTTTCTGGTGTCATTCCAAAGGAGCTTGGCAACTGTACAAATTTGGATACTCTGGCCCTCTACAAGAACAAGCTTGAAGGAGAGATACCAAAGGAGCTTGGGAGCCTTGTATTTCTAGAGAAGCTATATATGTACAGAAATATGCTGAGCGGGACAGTTCCAAGAGAGATTGGTAATCTTTCTCTTGCTACGGATGTTGATTTTTCTGAGAACTTGTTGTCTGGAGAGATACCAGTTGAGCTCAGTAAGATATCGGGGTTGCAATTGCTTTATCTTTTCGAGAACCAACTCACAGGTGTTATTCCAGAGGAGCTCACAAGCTTGACAAACCTGACAAGGCTCGACCTCTCTATAAATCATCTCACTGGTTCAATTCCAACTGGATTTCAATACATGAGTGAGCTGATCATGCTACAGTTGTTTAACAATATGTTGAGTGACATAATTCCTCAAGGACTTGGGGTGTACAGCCCACTTTGGGTGGTTGACTTATCTGAGAACTTCCTGACAGGAAGAATTCCTCGTCATGTCTGCAGAAATTCTAATCTGATTTTGTTAAACTTGGGGTCTAACAGGCTCACCGGTAATATCCCAACTGGGGTCACAAGCTGCAAGTCATTGGTGCAGCTTCGTCTGGTTGGAAACAACCTGACAGGGACTCTTCCAACCGAAATGTGCAAATTGGTTAACCTTTCTGCAGTTGAGTTGGACCAGAACAAGTTCAGCGGTGCCATTCCTCCAGCAATTGGTAACTGCAGAACTTTGCAGAGACTACTACTTGCAGACAACTACTTCACATCTGAGTTGCCAAGAGAGATTGGTGAGCTCTCGCAGTTGGTGATCTTCAATATCTCATCGAATTTACTTTCTGGCAGGATACCACCTGAAATTTTCAATTGTAAAATGCTTCAACGGCTTGATCTGAGTAGCAACAACTTTTCGGATGTCTTGCCAAGTGAGATAGGAGCACTTTCTCAGTTGGAGCTTCTCAAGCTTTCCGATAATCATCTTTCGGGGGATATCCCAGTTGCACTGGGAAATCTCTTGCATTTGACTGAGCTACAAATGGGTGGCAACTCATTCTCTGGGGGTATACCAGCTGAGTTGGGTGCCCTTTCAAGCCTCCAGATTGGATTAAATCTCAGTTATAACAATTTTTCTGGGGAGATACCTCCTCAACTTGGAAATCTTAGTTTACTGGAACTGCTTCTACTCAATAACAACCATTTGACTGGTGGAATTCCAGGTTCTTTTGACAACCTGTCAAGTTTACTTGGCTGCAACTTTTCATACAATGACTTGATAGGGCCTATTCCTTCTCTACCACTCTTTCAGAATATGGATGTTAGCAGCTTTGTTGGGAATAAAAGACTTTGCGGTGGCCCTCTTGGTGCTTGTGAAACACCATCTCCACTCTCTTTTCCTCCAGATGTGGCAAACAAAAGAACCCGCTTAGGGAAGATTATTGCTATTGTTGCGGCAGCCATTGGTGGTGTTTCTCTTATCTTAATCGTAATCATTATATATGTGATGAGACGTCCTGTAGACATTGTAGTAACTTCTGCGCAAGAAAAACCACTCTCTTCTCCAGTTTTGGACGTGTACTTTTCGCCAAAGGCAGGATTCACTTTTCAAGACTTGGTTATGGCGACTGAAAACTTTGATGAGAGATTTGCAATTGGAAGGGGAGCTTCTGGAACTGTGTATAAAGCTGTCTTGTTTCATGGTCATACAGTTGCTGTCAAGAAGGTAGTATCCAACAGGGAAGGAAACAATGTGGATAGTAGCTTTCATGCAGAGATTCTGACTTTAGGAAATATCAGGCATCGAAATATTGTGAAGCTGTATGGTTTCTGCCACCACCAGGGTTCCAATCTTCTGCTTTATGAGTACATGGAAAGGGGCAGCTTAGGAGAATTACTTCATGGAAAATCTTGTAATCTTGATTGGCAAACGAGGTTCAATATTGCTCTTGGGGCTGCCCAGGGTCTCTCTTATCTGCATCATGATTGCAAACCTCGGATCTTTCACCGTGATATAAAGTCCAACAACATTCTCCTCGATGAAATGTTTGAAGCTCATGTTGGAGATTTTGGGTTAGCAAAGGTGATTGACATGCCCTATTCAAAGTCAATGTCTGCAGTAGCAGGTTCTTATGGATACATAGCCCCTGAGTATGCATACACTATGAAAGTCACAGAAAAATGCGACATCTACAGCTATGGAGTGGTCCTTCTGGAGTTGCTGACTGGTAAAACACCAGTACAATCAGTAGACGAAGGCGGCGACCTGGTAACTTGGGTTAGAACTTACTTTCTCAACAATTCATTGTCATCAGAAGTACTCGATGCTCGATTGAATTTACAAGATGAAGATGAAGCTGAAGCTGCTACTACTGTCTCTCACATGATTACTGTCTTGAAAATTGCTTTGTTGTGCACAAGCATGTCTCCTTTTGAACGCCCGACCATGAGAGAAGTTGTTTCCATGCTTCTTGGGTCCAATGAGAAAGAAACAGTACAGTTTGCTAATGAGTACATCCCCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

1101

Amino Acids

120.14

Weight (kDa)

5.09

Isoelectric Point (pI)

34.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 27 - 67 8.5e-13 Leucine rich repeat N-terminal domain
LRR_14 PF23598 77 - 197 1.4e-12 Leucine-rich repeat region
LRR_14 PF23598 186 - 294 2.6e-09 Leucine-rich repeat region
LRR_14 PF23598 211 - 311 3.2e-08 Leucine-rich repeat region
LRR_14 PF23598 523 - 627 1.1e-08 Leucine-rich repeat region
LRR_8 PF13855 554 - 611 4.2e-06 Leucine rich repeat
Pkinase PF00069 810 - 1080 1.8e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 810 - 1082 1.3e-43 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011826)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G63930 AT5G63930
fragaria_vesca FvH4_6g01320 FvH4_6g01320
malus_domestica MD04G1235000.v1.1 MD12G1253000.v1.1
prunus_persica Prupe.6G354900_v2.0.a1 Prupe.6G354900_v2.0.a1
pyrus_communis pycom04g20870 pycom12g23210
rosa_chinensis RchiOBHm_Chr3g0448891
rosa_laevigata RLG00000025869
rosa_multiflora Rmu_co8340729.1_g000001 Rmu_sc0003116.1_g000020
rosa_rugosa Rorug02G0617000
rosa_samantha Rh3AG015200 Rh3CG013900 Rh3DG015500
rosa_wichuraiana Rw3G001130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1902
AatII GACGTC 1 cut(s) 2293
Acc16I TGCGCA 1 cut(s) 2319
Acc36I ACCTGC 2 cut(s) 358, 2891
AccB1I GGYRCC 2 cut(s) 1481, 1862
AccB7I CCANNNNNTGG 2 cut(s) 275, 1499
AccBSI CCGCTC 1 cut(s) 903
AccI GTMKAC 5 cut(s) 334, 424, 1846, 2298, 3027
AciI CCGC 8 cut(s) 218, 687, 903, 1479, 2130, 2203, 2231, 3036
AclWI GGATC 1 cut(s) 2783
AcoI YGGCCR 1 cut(s) 136
AcuI CTGAAG 2 cut(s) 360, 3156
AcyI GRCGYC 1 cut(s) 2290
AdeI CACNNNGTG 1 cut(s) 176
AfaI GTAC 9 cut(s) 790, 887, 1220, 2357, 2645, 3018, 3096, 3273, 3290
AflIII ACRYGT 1 cut(s) 2352
AgeI ACCGGT 1 cut(s) 1334
AhdI GACNNNNNGTC 1 cut(s) 2014
AjiI CACGTC 1 cut(s) 2353
AjnI CCWGG 4 cut(s) 1996, 2616, 2736, 3042
AjuI GAANNNNNNNTTGG 2 cut(s) 2820, 2852
Alw21I GWGCWC 5 cut(s) 996, 1068, 1581, 1723, 3194
Alw26I GTCTC 6 cut(s) 231, 1516, 2281, 2747, 3157, 3207
Alw44I GTGCAC 1 cut(s) 3190
AlwI GGATC 1 cut(s) 2783
AlwNI CAGNNNCTG 1 cut(s) 2492
Ama87I CYCGRG 1 cut(s) 716
AoxI GGCC 5 cut(s) 136, 473, 807, 2060, 2134
ApaLI GTGCAC 1 cut(s) 3190
ApeKI GCWGC 8 cut(s) 1362, 1378, 2031, 2103, 2234, 2658, 2732, 3140
ArsI GACNNNNNNTTYG 2 cut(s) 2873, 2905
AsiGI ACCGGT 1 cut(s) 1334
Asp700I GAANNNNTTC 5 cut(s) 499, 587, 1472, 1959, 2673
AspLEI GCGC 1 cut(s) 2320
AspS9I GGNCC 8 cut(s) 473, 545, 808, 1462, 2060, 2135, 2983, 3252
AsuC2I CCSGG 1 cut(s) 593
AsuHPI GGTGA 7 cut(s) 281, 650, 1324, 1586, 1603, 2774, 2872
AvaI CYCGRG 1 cut(s) 716
AvaII GGWCC 4 cut(s) 545, 1462, 2983, 3252
BaeGI GKGCMC 2 cut(s) 1867, 3194
BalI TGGCCA 1 cut(s) 138
BanI GGYRCC 2 cut(s) 1481, 1862
BanII GRGCYC 3 cut(s) 996, 1068, 1581
BarI GAAGNNNNNNTAC 2 cut(s) 822, 854
BbsI GAAGAC 1 cut(s) 348
Bbv12I GWGCWC 5 cut(s) 996, 1068, 1581, 1723, 3194
BbvI GCAGC 8 cut(s) 1349, 1390, 2018, 2115, 2246, 2670, 2719, 3127
BccI CCATC 3 cut(s) 147, 174, 2167
BceAI ACGGC 1 cut(s) 1682
BciT130I CCWGG 4 cut(s) 1998, 2618, 2738, 3044
BciVI GTATCC 5 cut(s) 222, 793, 1623, 2518, 2906
BclI TGATCA 2 cut(s) 85, 1158
BcnI CCSGG 1 cut(s) 593
BcoDI GTCTC 6 cut(s) 231, 1516, 2281, 2747, 3157, 3207
BfaI CTAG 3 cut(s) 104, 668, 869
BfmI CTRYAG 8 cut(s) 33, 1166, 1285, 1449, 1507, 2295, 2892, 2968
BfuAI ACCTGC 2 cut(s) 358, 2891
BfuI GTATCC 5 cut(s) 222, 793, 1623, 2518, 2906
BlpI GCTNAGC 1 cut(s) 899
BmcAI AGTACT 1 cut(s) 3096
Bme1390I CCNGG 5 cut(s) 593, 1998, 2618, 2738, 3044
Bme18I GGWCC 4 cut(s) 545, 1462, 2983, 3252
BmeRI GACNNNNNGTC 1 cut(s) 2014
BmeT110I CYCGRG 1 cut(s) 716
BmgBI CACGTC 1 cut(s) 2353
BmgT120I GGNCC 8 cut(s) 473, 545, 808, 1462, 2060, 2135, 2983, 3252
BmiI GGNNCC 5 cut(s) 857, 1483, 1864, 2623, 3253
BmrFI CCNGG 5 cut(s) 593, 1998, 2618, 2738, 3044
BmrI ACTGGG 3 cut(s) 1359, 1772, 1796
BmsI GCATC 3 cut(s) 2587, 2764, 3091
BmuI ACTGGG 3 cut(s) 1359, 1772, 1796
BpiI GAAGAC 1 cut(s) 348
BpmI CTGGAG 6 cut(s) 996, 1476, 1862, 2163, 2325, 3011
Bpu10I CCTNAGC 2 cut(s) 2206, 2662
Bpu1102I GCTNAGC 1 cut(s) 899
BpuEI CTTGAG 3 cut(s) 256, 1188, 1727
BpuMI CCSGG 1 cut(s) 593
BsaBI GATNNNNATC 1 cut(s) 1758
BsaHI GRCGYC 1 cut(s) 2290
BsaI GGTCTC 1 cut(s) 2747
BsaJI CCNNGG 4 cut(s) 2617, 2736, 2737, 2771
BsaWI WCCGGW 1 cut(s) 1334
BsaXI ACNNNNNCTCC 2 cut(s) 2146, 2176
Bse118I RCCGGY 1 cut(s) 1334
Bse8I GATNNNNATC 1 cut(s) 1758
BseBI CCWGG 4 cut(s) 1998, 2618, 2738, 3044
BseDI CCNNGG 4 cut(s) 2617, 2736, 2737, 2771
BseGI GGATG 5 cut(s) 185, 952, 1702, 2101, 3291
BseJI GATNNNNATC 1 cut(s) 1758
BseRI GAGGAG 4 cut(s) 89, 1076, 1917, 2801
BseSI GKGCMC 2 cut(s) 1867, 3194
BseXI GCAGC 8 cut(s) 1349, 1390, 2018, 2115, 2246, 2670, 2719, 3127
BsgI GTGCAG 1 cut(s) 1397
BshFI GGCC 5 cut(s) 138, 475, 809, 2062, 2136
BshNI GGYRCC 2 cut(s) 1481, 1862
BshTI ACCGGT 1 cut(s) 1334
BsiHKAI GWGCWC 5 cut(s) 996, 1068, 1581, 1723, 3194
BsiHKCI CYCGRG 1 cut(s) 716
BsiSI CCGG 3 cut(s) 215, 592, 1335
BslFI GGGAC 2 cut(s) 919, 1423
BsmAI GTCTC 6 cut(s) 231, 1516, 2281, 2747, 3157, 3207
BsmBI CGTCTC 1 cut(s) 2281
BsmFI GGGAC 2 cut(s) 919, 1423
BsmI GAATGC 1 cut(s) 717
BsnI GGCC 5 cut(s) 138, 475, 809, 2062, 2136
Bso31I GGTCTC 1 cut(s) 2747
BsoBI CYCGRG 1 cut(s) 716
Bsp1286I GDGCHC 6 cut(s) 996, 1068, 1581, 1723, 1867, 3194
Bsp1407I TGTACA 3 cut(s) 788, 885, 1218
Bsp143I GATC 5 cut(s) 85, 1158, 1593, 1672, 2775
Bsp1720I GCTNAGC 1 cut(s) 899
BspACI CCGC 8 cut(s) 218, 687, 903, 1479, 2130, 2203, 2231, 3036
BspANI GGCC 5 cut(s) 138, 475, 809, 2062, 2136
BspHI TCATGA 1 cut(s) 2758
BspLI GGNNCC 5 cut(s) 857, 1483, 1864, 2623, 3253
BspMAI CTGCAG 4 cut(s) 1289, 1453, 1511, 2896
BspMI ACCTGC 2 cut(s) 358, 2891
BspPI GGATC 1 cut(s) 2783
BspT107I GGYRCC 2 cut(s) 1481, 1862
BspTNI GGTCTC 1 cut(s) 2747
BsrBI CCGCTC 1 cut(s) 903
BsrFI RCCGGY 1 cut(s) 1334
BsrGI TGTACA 3 cut(s) 788, 885, 1218
BssAI RCCGGY 1 cut(s) 1334
BssECI CCNNGG 4 cut(s) 2617, 2736, 2737, 2771
BssMI GATC 5 cut(s) 85, 1158, 1593, 1672, 2775
BssNAI GTATAC 2 cut(s) 335, 1847
BssNI GRCGYC 1 cut(s) 2290
Bst1107I GTATAC 2 cut(s) 335, 1847
Bst2UI CCWGG 4 cut(s) 1998, 2618, 2738, 3044
Bst6I CTCTTC 2 cut(s) 1419, 2341
BstACI GRCGYC 1 cut(s) 2290
BstAUI TGTACA 3 cut(s) 788, 885, 1218
BstF5I GGATG 5 cut(s) 185, 952, 1702, 2101, 3291
BstHHI GCGC 1 cut(s) 2320
BstKTI GATC 5 cut(s) 88, 1161, 1596, 1675, 2778
BstMAI GTCTC 6 cut(s) 231, 1516, 2281, 2747, 3157, 3207
BstMBI GATC 5 cut(s) 85, 1158, 1593, 1672, 2775
BstMWI GCNNNNNNNGC 6 cut(s) 1162, 1666, 1744, 2228, 2729, 3140
BstNI CCWGG 4 cut(s) 1998, 2618, 2738, 3044
BstNSI RCATGY 2 cut(s) 2872, 3202
BstSCI CCNGG 5 cut(s) 591, 1996, 2616, 2736, 3042
BstSFI CTRYAG 8 cut(s) 33, 1166, 1285, 1449, 1507, 2295, 2892, 2968
BstSLI GKGCMC 2 cut(s) 1867, 3194
BstV1I GCAGC 8 cut(s) 1349, 1390, 2018, 2115, 2246, 2670, 2719, 3127
BstV2I GAAGAC 1 cut(s) 348
BstX2I RGATCY 1 cut(s) 2775
BstXI CCANNNNNNTGG 2 cut(s) 418, 1985
BstYI RGATCY 1 cut(s) 2775
BstZ17I GTATAC 2 cut(s) 335, 1847
BsuI GTATCC 5 cut(s) 222, 793, 1623, 2518, 2906
BsuRI GGCC 5 cut(s) 138, 475, 809, 2062, 2136
BtrI CACGTC 1 cut(s) 2353
BtsCI GGATG 5 cut(s) 185, 952, 1702, 2101, 3291
BtsI GCAGTG 1 cut(s) 548
BtsIMutI CAGTG 5 cut(s) 21, 166, 548, 1116, 1784
BveI ACCTGC 2 cut(s) 358, 2891
CaiI CAGNNNCTG 1 cut(s) 2492
CciI TCATGA 1 cut(s) 2758
CfoI GCGC 1 cut(s) 2320
Cfr10I RCCGGY 1 cut(s) 1334
Cfr13I GGNCC 8 cut(s) 473, 545, 808, 1462, 2060, 2135, 2983, 3252
CsiI ACCWGGT 1 cut(s) 3042
Csp6I GTAC 9 cut(s) 789, 886, 1219, 2356, 2644, 3017, 3095, 3272, 3289
CspAI ACCGGT 1 cut(s) 1334
CviQI GTAC 9 cut(s) 789, 886, 1219, 2356, 2644, 3017, 3095, 3272, 3289
DpnI GATC 5 cut(s) 87, 1160, 1595, 1674, 2777
DpnII GATC 5 cut(s) 85, 1158, 1593, 1672, 2775
DraI TTTAAA 1 cut(s) 381
DraIII CACNNNGTG 1 cut(s) 176
DriI GACNNNNNGTC 1 cut(s) 2014
EaeI YGGCCR 1 cut(s) 136
Eam1104I CTCTTC 2 cut(s) 1419, 2341
Eam1105I GACNNNNNGTC 1 cut(s) 2014
EarI CTCTTC 2 cut(s) 1419, 2341
Ecl136II GAGCTC 3 cut(s) 994, 1066, 1579
Eco24I GRGCYC 3 cut(s) 996, 1068, 1581
Eco31I GGTCTC 1 cut(s) 2747
Eco32I GATATC 3 cut(s) 12, 1005, 1774
Eco47I GGWCC 4 cut(s) 545, 1462, 2983, 3252
Eco53kI GAGCTC 3 cut(s) 994, 1066, 1579
Eco57I CTGAAG 2 cut(s) 360, 3156
Eco88I CYCGRG 1 cut(s) 716
EcoICRI GAGCTC 3 cut(s) 994, 1066, 1579
EcoO109I RGGNCCY 2 cut(s) 473, 2060
EcoRI GAATTC 4 cut(s) 154, 604, 1269, 1992
EcoRII CCWGG 4 cut(s) 1996, 2616, 2736, 3042
EcoRV GATATC 3 cut(s) 12, 1005, 1774
EcoT22I ATGCAT 1 cut(s) 2935
EcoT38I GRGCYC 3 cut(s) 996, 1068, 1581
Esp3I CGTCTC 1 cut(s) 2281
FalI AAGNNNNNCTT 4 cut(s) 610, 642, 2428, 2460
FaqI GGGAC 2 cut(s) 919, 1423
FauI CCCGC 2 cut(s) 896, 2210
FbaI TGATCA 2 cut(s) 85, 1158
FblI GTMKAC 5 cut(s) 334, 424, 1846, 2298, 3027
FokI GGATG 5 cut(s) 192, 959, 1709, 2108, 3278
FriOI GRGCYC 3 cut(s) 996, 1068, 1581
FspBI CTAG 3 cut(s) 104, 668, 869
FspI TGCGCA 1 cut(s) 2319
GlaI GCGC 1 cut(s) 2319
GsuI CTGGAG 6 cut(s) 996, 1476, 1862, 2163, 2325, 3011
HaeIII GGCC 5 cut(s) 138, 475, 809, 2062, 2136
HapII CCGG 3 cut(s) 215, 592, 1335
HhaI GCGC 1 cut(s) 2320
Hin1I GRCGYC 1 cut(s) 2290
Hin6I GCGC 1 cut(s) 2318
HinP1I GCGC 1 cut(s) 2318
HincII GTYRAC 3 cut(s) 127, 1240, 1441
HindII GTYRAC 3 cut(s) 127, 1240, 1441
HindIII AAGCTT 5 cut(s) 619, 649, 824, 1072, 1745
HinfI GANTC 7 cut(s) 68, 164, 247, 286, 1411, 2376, 2554
HpaI GTTAAC 1 cut(s) 1441
HpaII CCGG 3 cut(s) 215, 592, 1335
HphI GGTGA 7 cut(s) 281, 650, 1324, 1586, 1603, 2774, 2872
HpyCH4IV ACGT 3 cut(s) 556, 2290, 2352
HpyF10VI GCNNNNNNNGC 6 cut(s) 1162, 1666, 1744, 2228, 2729, 3140
HpySE526I ACGT 3 cut(s) 556, 2290, 2352
Hsp92I GRCGYC 1 cut(s) 2290
HspAI GCGC 1 cut(s) 2318
Ksp22I TGATCA 2 cut(s) 85, 1158
KspAI GTTAAC 1 cut(s) 1441
Kzo9I GATC 5 cut(s) 85, 1158, 1593, 1672, 2775
LmnI GCTCC 8 cut(s) 76, 775, 847, 855, 1063, 1718, 1735, 2441
Lsp1109I GCAGC 8 cut(s) 1349, 1390, 2018, 2115, 2246, 2670, 2719, 3127
LweI GCATC 3 cut(s) 2587, 2764, 3091
MabI ACCWGGT 1 cut(s) 3042
MaeI CTAG 3 cut(s) 104, 668, 869
MaeII ACGT 3 cut(s) 556, 2290, 2352
MaeIII GTNAC 7 cut(s) 46, 1190, 1354, 1502, 2308, 2947, 3046
MalI GATC 5 cut(s) 87, 1160, 1595, 1674, 2777
MbiI CCGCTC 1 cut(s) 903
MboI GATC 5 cut(s) 85, 1158, 1593, 1672, 2775
MfeI CAATTG 5 cut(s) 515, 1016, 1497, 1648, 2430
MflI RGATCY 1 cut(s) 2775
MhlI GDGCHC 6 cut(s) 996, 1068, 1581, 1723, 1867, 3194
MlsI TGGCCA 1 cut(s) 138
MluNI TGGCCA 1 cut(s) 138
MlyI GAGTC 1 cut(s) 1405
MmeI TCCRAC 9 cut(s) 131, 1154, 1372, 1440, 1442, 1713, 2535, 2818, 2823
Mox20I TGGCCA 1 cut(s) 138
Mph1103I ATGCAT 1 cut(s) 2935
MroXI GAANNNNTTC 5 cut(s) 499, 587, 1472, 1959, 2673
MscI TGGCCA 1 cut(s) 138
MseI TTAA 9 cut(s) 81, 380, 683, 734, 1176, 1310, 1440, 1889, 2261
MslI CAYNNNNRTG 1 cut(s) 3197
Msp20I TGGCCA 1 cut(s) 138
MspA1I CMGCKG 2 cut(s) 1479, 1853
MspI CCGG 3 cut(s) 215, 592, 1335
MspR9I CCNGG 5 cut(s) 593, 1998, 2618, 2738, 3044
MunI CAATTG 5 cut(s) 515, 1016, 1497, 1648, 2430
Mva1269I GAATGC 1 cut(s) 717
MvaI CCWGG 4 cut(s) 1998, 2618, 2738, 3044
MwoI GCNNNNNNNGC 6 cut(s) 1162, 1666, 1744, 2228, 2729, 3140
NciI CCSGG 1 cut(s) 593
NdeII GATC 5 cut(s) 85, 1158, 1593, 1672, 2775
NlaIV GGNNCC 5 cut(s) 857, 1483, 1864, 2623, 3253
NmuCI GTSAC 3 cut(s) 1190, 1354, 2947
NsbI TGCGCA 1 cut(s) 2319
NsiI ATGCAT 1 cut(s) 2935
NspI RCATGY 2 cut(s) 2872, 3202
PaeR7I CTCGAG 1 cut(s) 716
PagI TCATGA 1 cut(s) 2758
PasI CCCWGGG 1 cut(s) 2737
PcsI WCGNNNNNNNCGW 1 cut(s) 3036
PctI GAATGC 1 cut(s) 717
PdmI GAANNNNTTC 5 cut(s) 499, 587, 1472, 1959, 2673
PfeI GAWTC 6 cut(s) 68, 164, 247, 286, 2376, 2554
PflMI CCANNNNNTGG 2 cut(s) 275, 1499
PinAI ACCGGT 1 cut(s) 1334
PleI GAGTC 1 cut(s) 1405
PpsI GAGTC 1 cut(s) 1405
PsiI TTATAA 1 cut(s) 1902
Psp124BI GAGCTC 3 cut(s) 996, 1068, 1581
Psp6I CCWGG 4 cut(s) 1996, 2616, 2736, 3042
PspGI CCWGG 4 cut(s) 1996, 2616, 2736, 3042
PspN4I GGNNCC 5 cut(s) 857, 1483, 1864, 2623, 3253
PspPI GGNCC 8 cut(s) 473, 545, 808, 1462, 2060, 2135, 2983, 3252
PsrI GAACNNNNNNTAC 2 cut(s) 1947, 1979
PstI CTGCAG 4 cut(s) 1289, 1453, 1511, 2896
PstNI CAGNNNCTG 1 cut(s) 2492
PsuI RGATCY 1 cut(s) 2775
PvuII CAGCTG 1 cut(s) 1853
RsaI GTAC 9 cut(s) 790, 887, 1220, 2357, 2645, 3018, 3096, 3273, 3290
RsaNI GTAC 9 cut(s) 789, 886, 1219, 2356, 2644, 3017, 3095, 3272, 3289
RseI CAYNNNNRTG 1 cut(s) 3197
SacI GAGCTC 3 cut(s) 996, 1068, 1581
SaqAI TTAA 9 cut(s) 81, 380, 683, 734, 1176, 1310, 1440, 1889, 2261
Sau3AI GATC 5 cut(s) 85, 1158, 1593, 1672, 2775
Sau96I GGNCC 8 cut(s) 473, 545, 808, 1462, 2060, 2135, 2983, 3252
ScaI AGTACT 1 cut(s) 3096
SchI GAGTC 1 cut(s) 1405
ScrFI CCNGG 5 cut(s) 593, 1998, 2618, 2738, 3044
SduI GDGCHC 6 cut(s) 996, 1068, 1581, 1723, 1867, 3194
SexAI ACCWGGT 1 cut(s) 3042
SfaNI GCATC 3 cut(s) 2587, 2764, 3091
SfcI CTRYAG 8 cut(s) 33, 1166, 1285, 1449, 1507, 2295, 2892, 2968
Sfr274I CTCGAG 1 cut(s) 716
SinI GGWCC 4 cut(s) 545, 1462, 2983, 3252
SlaI CTCGAG 1 cut(s) 716
SmiMI CAYNNNNRTG 1 cut(s) 3197
SmlI CTYRAG 4 cut(s) 235, 716, 1203, 1742
SmoI CTYRAG 4 cut(s) 235, 716, 1203, 1742
SsiI CCGC 8 cut(s) 218, 687, 903, 1479, 2130, 2203, 2231, 3036
SspI AATATT 2 cut(s) 2587, 2719
SspMI CTAG 3 cut(s) 104, 668, 869
SstI GAGCTC 3 cut(s) 996, 1068, 1581
StyD4I CCNGG 5 cut(s) 591, 1996, 2616, 2736, 3042
TaiI ACGT 3 cut(s) 559, 2293, 2355
TaqI TCGA 8 cut(s) 717, 1032, 1095, 1610, 2581, 2814, 3099, 3106
TatI WGTACW 9 cut(s) 788, 885, 1218, 2355, 2643, 3016, 3094, 3271, 3288
TauI GCSGC 2 cut(s) 2234, 3039
TfiI GAWTC 6 cut(s) 68, 164, 247, 286, 2376, 2554
Tru1I TTAA 9 cut(s) 81, 380, 683, 734, 1176, 1310, 1440, 1889, 2261
Tru9I TTAA 9 cut(s) 81, 380, 683, 734, 1176, 1310, 1440, 1889, 2261
TscAI CASTG 5 cut(s) 21, 166, 555, 1123, 1791
TseFI GTSAC 3 cut(s) 1190, 1354, 2947
TseI GCWGC 8 cut(s) 1362, 1378, 2031, 2103, 2234, 2658, 2732, 3140
Tsp45I GTSAC 3 cut(s) 1190, 1354, 2947
TspGWI ACGGA 2 cut(s) 144, 959
TspRI CASTG 5 cut(s) 21, 166, 555, 1123, 1791
Van91I CCANNNNNTGG 2 cut(s) 275, 1499
VneI GTGCAC 1 cut(s) 3190
VpaK11BI GGWCC 4 cut(s) 545, 1462, 2983, 3252
XbaI TCTAGA 2 cut(s) 103, 868
XceI RCATGY 2 cut(s) 2872, 3202
XcmI CCANNNNNNNNNTGG 1 cut(s) 1985
XhoI CTCGAG 1 cut(s) 716
XmiI GTMKAC 5 cut(s) 334, 424, 1846, 2298, 3027
XmnI GAANNNNTTC 5 cut(s) 499, 587, 1472, 1959, 2673
XspI CTAG 3 cut(s) 104, 668, 869
ZraI GACGTC 1 cut(s) 2291
ZrmI AGTACT 1 cut(s) 3096
Zsp2I ATGCAT 1 cut(s) 2935
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.