AT5G64160

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0014473
Physical Location & Seq
Forward (+)
25670930 .. 25672710
1781 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G64160.1

Sequence Viewer

Length: 684 bp
ATGAGCTTGACGCTGCTTCAAGGCTATTCTTCTGCTGAAGAAGAAGAAGCAGAGGAGAGAGCTTTTGGGGATTACGACAACTCCGACGAAGATGGGGATAATGATGTTCGGAGATATGAATCGTCTTCTGTGTTTGATTTCTCTGCATCTGCCTCCTCCGCTAAAAACGCCGGCCTTCCTTCAGCTGACGACGTTTTCTCTCAGATTTCAGGGCCACCGGAGTTTCTAAACAACCGCACAGAAGCAGATAATGAAGCTTCAGCTAGAGATGCAGAACATGCAAATCGTATTAGTCGCAAAAAGAAGAAAGTTAAACCAAAAGGTGTTGTCATGGAAGCAAAACCTCAACTAGTAGGAATCCATGAGAGAGTAAGAAACGATATTGATGCACCCCCATCTTCAGAGAGTGGTGAAAAGCGGATATCTACTGCAACCAATCCTAATGCAGAAGAATCTGCAGATCTCTTGAGGATGTGTGTGCAATGCGGTGTACCGAAAACATATACAAGCGCAAGAGGAATGGTATGCCCGATTTGTGGTGACCGGCCATTACCGGACGTAGACGCGAAGAAGAAAGGCTCTACGATCAAAGATAAAGAGAAGAGCAAAAGAATGAGAGGACAATCTTCTCACGCTTCTTGGAAAAGTGAAACCGAGATGCAGCTTAGGCAAACTTTCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

227

Amino Acids

24.95

Weight (kDa)

5.33

Isoelectric Point (pI)

46.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014473)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64160
fragaria_vesca FvH4_2g12710
malus_domestica MD10G1051500.v1.1
prunus_persica Prupe.8G062400_v2.0.a1
pyrus_communis pycom10g03630
rosa_chinensis RchiOBHm_Chr6g0273461
rosa_laevigata RLG00000013615
rosa_multiflora Rmu_co8520659.1_g000001 Rmu_sc0005891.1_g000032 Rmu_sc0010380.1_g000009
rosa_roxburghii Rroxscaffold_7G00195310
rosa_rugosa Rorug06G0075500
rosa_samantha Rh6AG190200 Rh6BG193700 Rh6CG193200 Rh6DG184800
rosa_wichuraiana Rw6G016640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 561
AccII CGCG 1 cut(s) 566
AciI CCGC 4 cut(s) 159, 235, 418, 486
AcoI YGGCCR 1 cut(s) 545
AcuI CTGAAG 4 cut(s) 57, 165, 243, 384
AfaI GTAC 1 cut(s) 492
AfiI CCNNNNNNNGG 1 cut(s) 536
AgsI TTSAA 1 cut(s) 20
AhlI ACTAGT 1 cut(s) 349
AluBI AGCT 6 cut(s) 6, 62, 185, 257, 263, 664
AluI AGCT 6 cut(s) 6, 62, 185, 257, 263, 664
AoxI GGCC 3 cut(s) 172, 212, 545
ApeKI GCWGC 2 cut(s) 13, 661
AspLEI GCGC 1 cut(s) 512
AspS9I GGNCC 1 cut(s) 212
AsuHPI GGTGA 2 cut(s) 422, 551
BbsI GAAGAC 1 cut(s) 117
BbvI GCAGC 1 cut(s) 673
BccI CCATC 2 cut(s) 86, 403
BcgI CGANNNNNNTGC 2 cut(s) 368, 402
BcuI ACTAGT 1 cut(s) 349
BfaI CTAG 2 cut(s) 264, 350
BfmI CTRYAG 1 cut(s) 456
BglII AGATCT 1 cut(s) 460
BisI GCNGC 2 cut(s) 14, 662
BlsI GCNGC 2 cut(s) 15, 663
BmgT120I GGNCC 1 cut(s) 212
BmsI GCATC 4 cut(s) 155, 259, 376, 648
BpiI GAAGAC 1 cut(s) 117
Bpu10I CCTNAGC 1 cut(s) 665
BpuEI CTTGAG 1 cut(s) 487
BsaBI GATNNNNATC 1 cut(s) 118
BsaWI WCCGGW 2 cut(s) 217, 553
BsaXI ACNNNNNCTCC 2 cut(s) 65, 95
Bsc4I CCNNNNNNNGG 1 cut(s) 536
Bse118I RCCGGY 2 cut(s) 170, 543
Bse3DI GCAATG 1 cut(s) 488
Bse8I GATNNNNATC 1 cut(s) 118
BseGI GGATG 1 cut(s) 477
BseJI GATNNNNATC 1 cut(s) 118
BseLI CCNNNNNNNGG 1 cut(s) 536
BseMI GCAATG 1 cut(s) 488
BseMII CTCAG 1 cut(s) 215
BseRI GAGGAG 2 cut(s) 68, 145
BseXI GCAGC 1 cut(s) 673
Bsh1236I CGCG 1 cut(s) 566
BshFI GGCC 3 cut(s) 174, 214, 547
BsiSI CCGG 4 cut(s) 171, 218, 544, 554
BslI CCNNNNNNNGG 1 cut(s) 536
BsnI GGCC 3 cut(s) 174, 214, 547
Bsp143I GATC 2 cut(s) 460, 585
BspACI CCGC 4 cut(s) 159, 235, 418, 486
BspANI GGCC 3 cut(s) 174, 214, 547
BspCNI CTCAG 1 cut(s) 214
BspFNI CGCG 1 cut(s) 566
BspMAI CTGCAG 1 cut(s) 460
BspQI GCTCTTC 1 cut(s) 596
BsrDI GCAATG 1 cut(s) 488
BsrFI RCCGGY 2 cut(s) 170, 543
BssAI RCCGGY 2 cut(s) 170, 543
BssMI GATC 2 cut(s) 460, 585
Bst6I CTCTTC 1 cut(s) 596
BstAPI GCANNNNNTGC 1 cut(s) 278
BstC8I GCNNGC 1 cut(s) 172
BstDEI CTNAG 2 cut(s) 201, 665
BstEII GGTNACC 1 cut(s) 539
BstF5I GGATG 1 cut(s) 477
BstFNI CGCG 1 cut(s) 566
BstHHI GCGC 1 cut(s) 512
BstKTI GATC 2 cut(s) 463, 588
BstMBI GATC 2 cut(s) 460, 585
BstMWI GCNNNNNNNGC 5 cut(s) 158, 167, 269, 278, 667
BstNSI RCATGY 1 cut(s) 281
BstPI GGTNACC 1 cut(s) 539
BstSFI CTRYAG 1 cut(s) 456
BstUI CGCG 1 cut(s) 566
BstV1I GCAGC 1 cut(s) 673
BstV2I GAAGAC 1 cut(s) 117
BstX2I RGATCY 1 cut(s) 460
BstYI RGATCY 1 cut(s) 460
BsuRI GGCC 3 cut(s) 174, 214, 547
BtsCI GGATG 1 cut(s) 477
Cac8I GCNNGC 1 cut(s) 172
CfoI GCGC 1 cut(s) 512
Cfr10I RCCGGY 2 cut(s) 170, 543
Cfr13I GGNCC 1 cut(s) 212
CseI GACGC 2 cut(s) 19, 572
Csp6I GTAC 1 cut(s) 491
CviAII CATG 3 cut(s) 278, 331, 362
CviQI GTAC 1 cut(s) 491
DdeI CTNAG 2 cut(s) 201, 665
DpnI GATC 2 cut(s) 462, 587
DpnII GATC 2 cut(s) 460, 585
EaeI YGGCCR 1 cut(s) 545
Eam1104I CTCTTC 1 cut(s) 596
EarI CTCTTC 1 cut(s) 596
Eco32I GATATC 1 cut(s) 423
Eco57I CTGAAG 4 cut(s) 57, 165, 243, 384
Eco91I GGTNACC 1 cut(s) 539
EcoO65I GGTNACC 1 cut(s) 539
EcoRV GATATC 1 cut(s) 423
FaeI CATG 3 cut(s) 281, 334, 365
FaiI YATR 7 cut(s) 117, 279, 332, 363, 502, 504, 526
FatI CATG 3 cut(s) 277, 330, 361
FblI GTMKAC 1 cut(s) 561
Fnu4HI GCNGC 2 cut(s) 14, 662
FokI GGATG 1 cut(s) 484
Fsp4HI GCNGC 2 cut(s) 14, 662
FspBI CTAG 2 cut(s) 264, 350
GlaI GCGC 1 cut(s) 511
GluI GCNGC 2 cut(s) 14, 662
HaeIII GGCC 3 cut(s) 174, 214, 547
HapII CCGG 4 cut(s) 171, 218, 544, 554
HgaI GACGC 2 cut(s) 19, 572
HhaI GCGC 1 cut(s) 512
Hin1II CATG 3 cut(s) 281, 334, 365
Hin6I GCGC 1 cut(s) 510
HinP1I GCGC 1 cut(s) 510
HindIII AAGCTT 1 cut(s) 255
HinfI GANTC 3 cut(s) 119, 357, 452
HpaII CCGG 4 cut(s) 171, 218, 544, 554
HphI GGTGA 2 cut(s) 422, 551
Hpy166II GTNNAC 2 cut(s) 491, 562
Hpy188I TCNGA 4 cut(s) 85, 111, 204, 403
Hpy188III TCNNGA 1 cut(s) 466
Hpy8I GTNNAC 2 cut(s) 491, 562
Hpy99I CGWCG 2 cut(s) 89, 194
HpyAV CCTTC 2 cut(s) 185, 189
HpyCH4IV ACGT 2 cut(s) 192, 558
HpyCH4V TGCA 9 cut(s) 146, 272, 281, 389, 431, 446, 458, 481, 661
HpyF10VI GCNNNNNNNGC 5 cut(s) 158, 167, 269, 278, 667
HpyF3I CTNAG 2 cut(s) 201, 665
HpySE526I ACGT 2 cut(s) 192, 558
Hsp92II CATG 3 cut(s) 281, 334, 365
HspAI GCGC 1 cut(s) 510
KroI GCCGGC 1 cut(s) 170
KroNI GCCGGC 1 cut(s) 172
Kzo9I GATC 2 cut(s) 460, 585
LguI GCTCTTC 1 cut(s) 596
LpnPI CCDG 5 cut(s) 184, 195, 231, 557, 567
Lsp1109I GCAGC 1 cut(s) 673
LweI GCATC 4 cut(s) 155, 259, 376, 648
MaeI CTAG 2 cut(s) 264, 350
MaeII ACGT 2 cut(s) 192, 558
MaeIII GTNAC 1 cut(s) 539
MalI GATC 2 cut(s) 462, 587
MboI GATC 2 cut(s) 460, 585
MflI RGATCY 1 cut(s) 460
MmeI TCCRAC 1 cut(s) 108
MnlI CCTC 7 cut(s) 46, 163, 166, 354, 462, 509, 611
MroNI GCCGGC 1 cut(s) 170
MseI TTAA 1 cut(s) 312
MspA1I CMGCKG 1 cut(s) 185
MspI CCGG 4 cut(s) 171, 218, 544, 554
MvnI CGCG 1 cut(s) 566
MwoI GCNNNNNNNGC 5 cut(s) 158, 167, 269, 278, 667
NaeI GCCGGC 1 cut(s) 172
NdeII GATC 2 cut(s) 460, 585
NgoMIV GCCGGC 1 cut(s) 170
NlaIII CATG 3 cut(s) 281, 334, 365
NmuCI GTSAC 1 cut(s) 539
NspI RCATGY 1 cut(s) 281
PciSI GCTCTTC 1 cut(s) 596
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PdiI GCCGGC 1 cut(s) 172
PfeI GAWTC 3 cut(s) 119, 357, 452
PkrI GCNGC 2 cut(s) 15, 663
PspEI GGTNACC 1 cut(s) 539
PspPI GGNCC 1 cut(s) 212
PstI CTGCAG 1 cut(s) 460
PsuI RGATCY 1 cut(s) 460
PvuII CAGCTG 1 cut(s) 185
RsaI GTAC 1 cut(s) 492
RsaNI GTAC 1 cut(s) 491
SapI GCTCTTC 1 cut(s) 596
SaqAI TTAA 1 cut(s) 312
SatI GCNGC 2 cut(s) 14, 662
Sau3AI GATC 2 cut(s) 460, 585
Sau96I GGNCC 1 cut(s) 212
SfaNI GCATC 4 cut(s) 155, 259, 376, 648
SfcI CTRYAG 1 cut(s) 456
SmlI CTYRAG 1 cut(s) 466
SmoI CTYRAG 1 cut(s) 466
SpeI ACTAGT 1 cut(s) 349
SsiI CCGC 4 cut(s) 159, 235, 418, 486
SspMI CTAG 2 cut(s) 264, 350
TaiI ACGT 2 cut(s) 195, 561
TaqI TCGA 1 cut(s) 678
TfiI GAWTC 3 cut(s) 119, 357, 452
Tru1I TTAA 1 cut(s) 312
Tru9I TTAA 1 cut(s) 312
TseFI GTSAC 1 cut(s) 539
TseI GCWGC 2 cut(s) 13, 661
Tsp45I GTSAC 1 cut(s) 539
TspDTI ATGAA 2 cut(s) 132, 267
XceI RCATGY 1 cut(s) 281
XmiI GTMKAC 1 cut(s) 561
XspI CTAG 2 cut(s) 264, 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.