AT5G66160

Receptor homology region transmembrane domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008527
Physical Location & Seq
Forward (+)
26445086 .. 26447433
2348 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G66160.1

Sequence Viewer

Length: 933 bp
ATGAGACTCGTCGTCTCAAGCTGTCTACTAGTTGCAGCTCCTTTTCTCTCCTCTCTGTTACGAGTCTCACTCGCCACTGTTGTCCTCAATTCCATCTCCGCCTCTTTTGCCGATCTCCCAGCCAAATTTGACGGCTCCGTGACCAAAAACGGAATCTGTGGAGCTCTATACGTCGCAGATCCTCTCGACGGTTGCTCACCGCTTCTCCACGCCGCCGCATCCAACTGGACGCAACACAGAACTACTAAGTTCGCTTTGATAATCAGAGGCGAATGTTCTTTTGAGGATAAGCTGCTCAATGCCCAGAACTCAGGTTTTCAAGCTGTGATTGTCTATGACAACATTGACAACGAAGATCTCATCGTCATGAAGGTGAACCCTCAGGACATTACAGTTGATGCAGTCTTCGTTTCAAATGTCGCCGGTGAGATTTTGAGAAAGTACGCGAGAGGCCGAGATGGTGAATGCTGCCTTAATCCGCCAGACAGAGGGAGCGCTTGGACTGTGTTGGCCATCTCCTTCTTCTCTCTCCTTCTTATAGTCACTTTCCTGTTGATTGCCTTCTTTGCACCCAGACACTGGACCCAATGGCGAGGGAGGCACACCAGGACCATCAGGTTAGATGCAAAGCTCGTCCACACACTCCCCTGCTTCACCTTCACTGATTCTGCTCACCACAAGGCCGGGGAAACATGTGCTATATGTCTCGAGGATTACAGATTTGGAGAAAGCCTCAGACTTCTCCCCTGCCAACATGCTTTTCACTTGAATTGCATCGACTCTTGGTTGACAAAATGGGGTACATCTTGCCCTGTGTGCAAGCATGACATAAGAACCGAGACTATGTCTTCTGAGGTACATAAACGAGAGAGTCCGAGAACAGATACAAGTACGAGTAGATTTGCCTTTGCCCAATCCAGTCAAAGCCGTTAG

Protein Analysis

310

Amino Acids

34.43

Weight (kDa)

7.96

Isoelectric Point (pI)

38.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PA PF02225 61 - 145 2.7e-12 PA domain
zf-RING_2 PF13639 231 - 274 4.5e-13 Ring finger domain
zf-RING_11 PF17123 232 - 259 2e-10 RING-like zinc finger
zf-C3HC4 PF00097 232 - 273 3.9e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 232 - 273 8.4e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 232 - 271 4.4e-07 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 579
AccI GTMKAC 1 cut(s) 25
AccII CGCG 1 cut(s) 446
AciI CCGC 5 cut(s) 99, 200, 213, 216, 479
AclWI GGATC 1 cut(s) 173
AcoI YGGCCR 1 cut(s) 510
AcsI RAATTY 1 cut(s) 125
AfaI GTAC 4 cut(s) 443, 802, 858, 892
AfeI AGCGCT 1 cut(s) 496
AfiI CCNNNNNNNGG 3 cut(s) 188, 488, 579
AflIII ACRYGT 1 cut(s) 692
AgsI TTSAA 3 cut(s) 320, 414, 769
AhdI GACNNNNNGTC 1 cut(s) 11
AhlI ACTAGT 1 cut(s) 28
AjnI CCWGG 1 cut(s) 605
AjuI GAANNNNNNNTTGG 2 cut(s) 744, 776
AluBI AGCT 6 cut(s) 21, 38, 164, 292, 323, 631
AluI AGCT 6 cut(s) 21, 38, 164, 292, 323, 631
Alw21I GWGCWC 1 cut(s) 166
Alw26I GTCTC 4 cut(s) 19, 70, 710, 833
AlwI GGATC 1 cut(s) 173
AlwNI CAGNNNCTG 1 cut(s) 579
Ama87I CYCGRG 1 cut(s) 707
Aor51HI AGCGCT 1 cut(s) 496
AoxI GGCC 3 cut(s) 451, 510, 681
ApeKI GCWGC 3 cut(s) 35, 292, 468
ApoI RAATTY 1 cut(s) 125
AspLEI GCGC 1 cut(s) 497
AspS9I GGNCC 2 cut(s) 582, 609
AsuC2I CCSGG 1 cut(s) 685
AsuHPI GGTGA 6 cut(s) 189, 385, 437, 473, 646, 665
AvaI CYCGRG 1 cut(s) 707
AvaII GGWCC 2 cut(s) 582, 609
AxyI CCTNAGG 1 cut(s) 381
BalI TGGCCA 1 cut(s) 512
BanII GRGCYC 1 cut(s) 166
BbsI GAAGAC 2 cut(s) 397, 840
Bbv12I GWGCWC 1 cut(s) 166
BbvI GCAGC 3 cut(s) 47, 279, 455
BccI CCATC 4 cut(s) 101, 452, 521, 620
BceAI ACGGC 2 cut(s) 148, 912
BciT130I CCWGG 1 cut(s) 607
BcnI CCSGG 1 cut(s) 685
BcoDI GTCTC 4 cut(s) 19, 70, 710, 833
BcuI ACTAGT 1 cut(s) 28
BfaI CTAG 1 cut(s) 29
BfoI RGCGCY 1 cut(s) 498
BglII AGATCT 1 cut(s) 355
BisI GCNGC 5 cut(s) 36, 213, 216, 293, 469
BlsI GCNGC 5 cut(s) 37, 214, 217, 294, 470
Bme1390I CCNGG 2 cut(s) 607, 685
Bme18I GGWCC 2 cut(s) 582, 609
BmeRI GACNNNNNGTC 1 cut(s) 11
BmeT110I CYCGRG 1 cut(s) 707
BmgT120I GGNCC 2 cut(s) 582, 609
BmiI GGNNCC 2 cut(s) 136, 584
BmrFI CCNGG 2 cut(s) 607, 685
BmsI GCATC 4 cut(s) 227, 388, 613, 783
BpiI GAAGAC 2 cut(s) 397, 840
BplI GAGNNNNNCTC 4 cut(s) 54, 86, 717, 749
BpuMI CCSGG 1 cut(s) 685
BsaJI CCNNGG 1 cut(s) 684
BsaXI ACNNNNNCTCC 2 cut(s) 189, 219
Bsc4I CCNNNNNNNGG 3 cut(s) 188, 488, 579
Bse118I RCCGGY 1 cut(s) 422
Bse1I ACTGG 3 cut(s) 230, 584, 918
Bse21I CCTNAGG 1 cut(s) 381
BseBI CCWGG 1 cut(s) 607
BseDI CCNNGG 1 cut(s) 684
BseGI GGATG 1 cut(s) 218
BseLI CCNNNNNNNGG 3 cut(s) 188, 488, 579
BseMII CTCAG 4 cut(s) 324, 395, 748, 843
BseNI ACTGG 3 cut(s) 230, 584, 918
BseRI GAGGAG 1 cut(s) 40
BseXI GCAGC 3 cut(s) 47, 279, 455
BseYI CCCAGC 1 cut(s) 118
Bsh1236I CGCG 1 cut(s) 446
BshFI GGCC 3 cut(s) 453, 512, 683
BsiHKAI GWGCWC 1 cut(s) 166
BsiHKCI CYCGRG 1 cut(s) 707
BsiSI CCGG 2 cut(s) 423, 684
BslI CCNNNNNNNGG 3 cut(s) 188, 488, 579
BsmAI GTCTC 4 cut(s) 19, 70, 710, 833
BsmBI CGTCTC 1 cut(s) 19
BsmI GAATGC 1 cut(s) 470
BsnI GGCC 3 cut(s) 453, 512, 683
BsoBI CYCGRG 1 cut(s) 707
Bsp1286I GDGCHC 1 cut(s) 166
Bsp143I GATC 3 cut(s) 112, 178, 355
BspACI CCGC 5 cut(s) 99, 200, 213, 216, 479
BspANI GGCC 3 cut(s) 453, 512, 683
BspCNI CTCAG 4 cut(s) 323, 394, 747, 844
BspFNI CGCG 1 cut(s) 446
BspHI TCATGA 1 cut(s) 366
BspLI GGNNCC 2 cut(s) 136, 584
BspPI GGATC 1 cut(s) 173
BsrFI RCCGGY 1 cut(s) 422
BsrI ACTGG 3 cut(s) 230, 584, 918
BssAI RCCGGY 1 cut(s) 422
BssECI CCNNGG 1 cut(s) 684
BssMI GATC 3 cut(s) 112, 178, 355
Bst2UI CCWGG 1 cut(s) 607
Bst4CI ACNGT 4 cut(s) 79, 191, 394, 505
BstC8I GCNNGC 1 cut(s) 821
BstDEI CTNAG 5 cut(s) 246, 310, 381, 734, 852
BstF5I GGATG 1 cut(s) 218
BstFNI CGCG 1 cut(s) 446
BstH2I RGCGCY 1 cut(s) 498
BstHHI GCGC 1 cut(s) 497
BstKTI GATC 3 cut(s) 115, 181, 358
BstMAI GTCTC 4 cut(s) 19, 70, 710, 833
BstMBI GATC 3 cut(s) 112, 178, 355
BstMWI GCNNNNNNNGC 4 cut(s) 107, 566, 598, 816
BstNI CCWGG 1 cut(s) 607
BstNSI RCATGY 2 cut(s) 696, 758
BstSCI CCNGG 2 cut(s) 605, 683
BstUI CGCG 1 cut(s) 446
BstV1I GCAGC 3 cut(s) 47, 279, 455
BstV2I GAAGAC 2 cut(s) 397, 840
BstX2I RGATCY 2 cut(s) 178, 355
BstYI RGATCY 2 cut(s) 178, 355
Bsu36I CCTNAGG 1 cut(s) 381
BsuRI GGCC 3 cut(s) 453, 512, 683
BtsCI GGATG 1 cut(s) 218
BtsIMutI CAGTG 3 cut(s) 75, 577, 660
Cac8I GCNNGC 1 cut(s) 821
CaiI CAGNNNCTG 1 cut(s) 579
CciI TCATGA 1 cut(s) 366
CfoI GCGC 1 cut(s) 497
Cfr10I RCCGGY 1 cut(s) 422
Cfr13I GGNCC 2 cut(s) 582, 609
CseI GACGC 1 cut(s) 238
Csp6I GTAC 4 cut(s) 442, 801, 857, 891
CviAII CATG 4 cut(s) 367, 693, 755, 824
CviQI GTAC 4 cut(s) 442, 801, 857, 891
DdeI CTNAG 5 cut(s) 246, 310, 381, 734, 852
DpnI GATC 3 cut(s) 114, 180, 357
DpnII GATC 3 cut(s) 112, 178, 355
DriI GACNNNNNGTC 1 cut(s) 11
EaeI YGGCCR 1 cut(s) 510
Eam1105I GACNNNNNGTC 1 cut(s) 11
EciI GGCGGA 2 cut(s) 88, 468
Ecl136II GAGCTC 1 cut(s) 164
Eco24I GRGCYC 1 cut(s) 166
Eco47I GGWCC 2 cut(s) 582, 609
Eco47III AGCGCT 1 cut(s) 496
Eco53kI GAGCTC 1 cut(s) 164
Eco81I CCTNAGG 1 cut(s) 381
Eco88I CYCGRG 1 cut(s) 707
EcoICRI GAGCTC 1 cut(s) 164
EcoRII CCWGG 1 cut(s) 605
EcoT38I GRGCYC 1 cut(s) 166
Esp3I CGTCTC 1 cut(s) 19
FaeI CATG 4 cut(s) 370, 696, 758, 827
FatI CATG 4 cut(s) 366, 692, 754, 823
FblI GTMKAC 1 cut(s) 25
Fnu4HI GCNGC 5 cut(s) 36, 213, 216, 293, 469
FokI GGATG 1 cut(s) 205
FriOI GRGCYC 1 cut(s) 166
Fsp4HI GCNGC 5 cut(s) 36, 213, 216, 293, 469
FspBI CTAG 1 cut(s) 29
GlaI GCGC 1 cut(s) 496
GluI GCNGC 5 cut(s) 36, 213, 216, 293, 469
GsaI CCCAGC 1 cut(s) 122
HaeII RGCGCY 1 cut(s) 498
HaeIII GGCC 3 cut(s) 453, 512, 683
HapII CCGG 2 cut(s) 423, 684
HgaI GACGC 1 cut(s) 238
HhaI GCGC 1 cut(s) 497
Hin1II CATG 4 cut(s) 370, 696, 758, 827
Hin6I GCGC 1 cut(s) 495
HinP1I GCGC 1 cut(s) 495
HincII GTYRAC 1 cut(s) 789
HindII GTYRAC 1 cut(s) 789
HinfI GANTC 6 cut(s) 6, 63, 153, 665, 779, 871
HpaII CCGG 2 cut(s) 423, 684
HphI GGTGA 6 cut(s) 189, 385, 437, 473, 646, 665
Hpy166II GTNNAC 4 cut(s) 26, 376, 637, 789
Hpy188I TCNGA 4 cut(s) 266, 737, 853, 876
Hpy188III TCNNGA 4 cut(s) 185, 367, 383, 707
Hpy8I GTNNAC 4 cut(s) 26, 376, 637, 789
Hpy99I CGWCG 3 cut(s) 14, 176, 191
HpyAV CCTTC 5 cut(s) 364, 529, 542, 571, 667
HpyCH4III ACNGT 4 cut(s) 79, 191, 394, 505
HpyCH4IV ACGT 1 cut(s) 171
HpyCH4V TGCA 6 cut(s) 35, 401, 569, 626, 774, 819
HpyF10VI GCNNNNNNNGC 4 cut(s) 107, 566, 598, 816
HpyF3I CTNAG 5 cut(s) 246, 310, 381, 734, 852
HpySE526I ACGT 1 cut(s) 171
Hsp92II CATG 4 cut(s) 370, 696, 758, 827
HspAI GCGC 1 cut(s) 495
Kzo9I GATC 3 cut(s) 112, 178, 355
LmnI GCTCC 4 cut(s) 43, 140, 161, 492
Lsp1109I GCAGC 3 cut(s) 47, 279, 455
LweI GCATC 4 cut(s) 227, 388, 613, 783
MaeI CTAG 1 cut(s) 29
MaeII ACGT 1 cut(s) 171
MaeIII GTNAC 3 cut(s) 57, 139, 541
MalI GATC 3 cut(s) 114, 180, 357
MboI GATC 3 cut(s) 112, 178, 355
MboII GAAGA 4 cut(s) 365, 397, 514, 840
MflI RGATCY 2 cut(s) 178, 355
MhlI GDGCHC 1 cut(s) 166
MlsI TGGCCA 1 cut(s) 512
MluCI AATT 3 cut(s) 88, 125, 769
MluNI TGGCCA 1 cut(s) 512
MlyI GAGTC 3 cut(s) 72, 773, 880
MmeI TCCRAC 1 cut(s) 246
Mox20I TGGCCA 1 cut(s) 512
MscI TGGCCA 1 cut(s) 512
MseI TTAA 1 cut(s) 474
MslI CAYNNNNRTG 2 cut(s) 365, 371
Msp20I TGGCCA 1 cut(s) 512
MspI CCGG 2 cut(s) 423, 684
MspR9I CCNGG 2 cut(s) 607, 685
Mva1269I GAATGC 1 cut(s) 470
MvaI CCWGG 1 cut(s) 607
MvnI CGCG 1 cut(s) 446
MwoI GCNNNNNNNGC 4 cut(s) 107, 566, 598, 816
NciI CCSGG 1 cut(s) 685
NdeII GATC 3 cut(s) 112, 178, 355
NlaIII CATG 4 cut(s) 370, 696, 758, 827
NlaIV GGNNCC 2 cut(s) 136, 584
NmeAIII GCCGAG 1 cut(s) 479
NmuCI GTSAC 2 cut(s) 139, 541
NspI RCATGY 2 cut(s) 696, 758
PaeR7I CTCGAG 1 cut(s) 707
PagI TCATGA 1 cut(s) 366
PciI ACATGT 1 cut(s) 692
PctI GAATGC 1 cut(s) 470
PfeI GAWTC 2 cut(s) 153, 665
PflFI GACNNNGTC 1 cut(s) 844
PflMI CCANNNNNTGG 1 cut(s) 579
PkrI GCNGC 5 cut(s) 37, 214, 217, 294, 470
PleI GAGTC 3 cut(s) 71, 773, 879
PpsI GAGTC 3 cut(s) 71, 773, 879
PscI ACATGT 1 cut(s) 692
Psp124BI GAGCTC 1 cut(s) 166
Psp6I CCWGG 1 cut(s) 605
PspFI CCCAGC 1 cut(s) 118
PspGI CCWGG 1 cut(s) 605
PspN4I GGNNCC 2 cut(s) 136, 584
PspPI GGNCC 2 cut(s) 582, 609
PstNI CAGNNNCTG 1 cut(s) 579
PsuI RGATCY 2 cut(s) 178, 355
PsyI GACNNNGTC 1 cut(s) 844
RsaI GTAC 4 cut(s) 443, 802, 858, 892
RsaNI GTAC 4 cut(s) 442, 801, 857, 891
RseI CAYNNNNRTG 2 cut(s) 365, 371
SacI GAGCTC 1 cut(s) 166
SaqAI TTAA 1 cut(s) 474
SatI GCNGC 5 cut(s) 36, 213, 216, 293, 469
Sau3AI GATC 3 cut(s) 112, 178, 355
Sau96I GGNCC 2 cut(s) 582, 609
SchI GAGTC 3 cut(s) 72, 773, 880
ScrFI CCNGG 2 cut(s) 607, 685
SduI GDGCHC 1 cut(s) 166
SfaNI GCATC 4 cut(s) 227, 388, 613, 783
Sfr274I CTCGAG 1 cut(s) 707
SgrAI CRCCGGYG 1 cut(s) 422
SinI GGWCC 2 cut(s) 582, 609
SlaI CTCGAG 1 cut(s) 707
SmiMI CAYNNNNRTG 2 cut(s) 365, 371
SmlI CTYRAG 2 cut(s) 16, 707
SmoI CTYRAG 2 cut(s) 16, 707
SpeI ACTAGT 1 cut(s) 28
Sse9I AATT 3 cut(s) 88, 125, 769
SsiI CCGC 5 cut(s) 99, 200, 213, 216, 479
SspMI CTAG 1 cut(s) 29
SstI GAGCTC 1 cut(s) 166
StyD4I CCNGG 2 cut(s) 605, 683
TaaI ACNGT 4 cut(s) 79, 191, 394, 505
TaiI ACGT 1 cut(s) 174
TaqI TCGA 3 cut(s) 186, 708, 777
TasI AATT 3 cut(s) 88, 125, 769
TauI GCSGC 2 cut(s) 215, 218
TfiI GAWTC 2 cut(s) 153, 665
Tru1I TTAA 1 cut(s) 474
Tru9I TTAA 1 cut(s) 474
TscAI CASTG 3 cut(s) 82, 584, 667
TseFI GTSAC 2 cut(s) 139, 541
TseI GCWGC 3 cut(s) 35, 292, 468
Tsp45I GTSAC 2 cut(s) 139, 541
TspDTI ATGAA 1 cut(s) 383
TspGWI ACGGA 2 cut(s) 127, 165
TspRI CASTG 3 cut(s) 82, 584, 667
Tth111I GACNNNGTC 1 cut(s) 844
Van91I CCANNNNNTGG 1 cut(s) 579
VpaK11BI GGWCC 2 cut(s) 582, 609
XapI RAATTY 1 cut(s) 125
XceI RCATGY 2 cut(s) 696, 758
XhoI CTCGAG 1 cut(s) 707
XmiI GTMKAC 1 cut(s) 25
XspI CTAG 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.