MD02G1129600.v1.1

Receptor homology region, transmembrane domain- and RING domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0008527
Physical Location & Seq
Reverse (-)
10469579 .. 10472718
3140 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1129600.v1.1.491

Sequence Viewer

Length: 876 bp
ATGAGGGAAGCCGTAGTGGGATTCTTTGTGGTTGTTTATCTTCAGCTTCTCCAACTTAGCTCAGCTAACATTGTTCTCCAACCATCTGGAATTCTTATCCCCGACCTTCCCGCCAAATATGCTGTTGCTGTCGGCGGCTCTGAAATATGTGGAGCTTTGCTTGTAGCAGACCCTTTGGATGCCTGCTTGCCTCTGCGCTATGGTGTTCAATCAAATGAAACTGATATAACAAGGTTTGCTTTAGTAGAAAGAGGTGAATGTACTTTTGAGGATAAAGTGCAACACGCTCAAAATGCCGGATTTCATGCTGTGATTGTTTACGATAATAGAAGAGAAAAATTGGTTCACATGACGATAAACAGCAAGAATGTTACAATTCGTGCTGTATTTGTTTCCAAAGCATCTGGTGAAATGCTAAAGGAACATGCTCGTGGGGAAGAAGGGGAATGCTGCATCTTCGTATTACCAAATGAAAAGGCCTGGTCGGTGTTCGCTGTATGTTTCATCTCATTTCTAACTGTATTGGGTCTCCTTGTGATTGCCCTCTGTGCTCAGAGAAACTGGAGCTGGACTACTCAAGGAAGAAACCAACTTCCTAAAAGTGCTGATACCAAGATTGTGGATGCTCTACCTTGCTTCACATTCAGTTCTGCCGGTTTGGATGAATGCCACACTGAAGAAACTTGTGCCATCTGCCTTGAGAATTACAAAGATTCCGAAATTCTTAAAGTTCTCCCCTGCCAACATGAATTTCATTCAAGCTGTGTGGATTCATGGCTGAAAAAATGGGGGAGAAACTGCCCCGTGTGCAAGAACGATATAAGAAAGAAAATTGCGAATGCTGAGATCAAGAGAAAAACCTGGTTATACAATTAG

Protein Analysis

292

Amino Acids

32.48

Weight (kDa)

6.65

Isoelectric Point (pI)

39.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PA PF02225 55 - 139 2.7e-14 PA domain
zf-rbx1 PF12678 223 - 271 6e-08 RING-H2 zinc finger domain
zf-RING_2 PF13639 228 - 271 2.4e-12 Ring finger domain
zf-C3HC4 PF00097 229 - 270 7.3e-11 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 229 - 270 3.2e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 229 - 256 5.8e-09 RING-like zinc finger
zf-RING_UBOX PF13445 229 - 268 7.4e-07 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 111, 135
AcsI RAATTY 3 cut(s) 90, 720, 749
AcuI CTGAAG 2 cut(s) 26, 696
AfaI GTAC 1 cut(s) 262
AgsI TTSAA 2 cut(s) 209, 759
AjnI CCWGG 2 cut(s) 479, 860
AjuI GAANNNNNNNTTGG 2 cut(s) 735, 767
AluBI AGCT 6 cut(s) 46, 60, 65, 155, 567, 762
AluI AGCT 6 cut(s) 46, 60, 65, 155, 567, 762
Alw21I GWGCWC 1 cut(s) 553
Alw26I GTCTC 1 cut(s) 533
AoxI GGCC 1 cut(s) 477
ApeKI GCWGC 1 cut(s) 450
ApoI RAATTY 3 cut(s) 90, 720, 749
AspLEI GCGC 1 cut(s) 198
AsuHPI GGTGA 2 cut(s) 266, 419
BauI CACGAG 1 cut(s) 429
Bbv12I GWGCWC 1 cut(s) 553
BbvI GCAGC 1 cut(s) 437
BccI CCATC 2 cut(s) 91, 698
BciT130I CCWGG 2 cut(s) 481, 862
BcoDI GTCTC 1 cut(s) 533
BisI GCNGC 2 cut(s) 136, 451
BlpI GCTNAGC 1 cut(s) 61
BlsI GCNGC 2 cut(s) 137, 452
Bme1390I CCNGG 2 cut(s) 481, 862
BmrFI CCNGG 2 cut(s) 481, 862
BmsI GCATC 4 cut(s) 169, 410, 462, 613
BpmI CTGGAG 1 cut(s) 583
Bpu1102I GCTNAGC 1 cut(s) 61
BpuEI CTTGAG 2 cut(s) 561, 719
BsaI GGTCTC 1 cut(s) 533
Bse118I RCCGGY 1 cut(s) 653
Bse1I ACTGG 1 cut(s) 566
BseBI CCWGG 2 cut(s) 481, 862
BseGI GGATG 3 cut(s) 184, 628, 667
BseMII CTCAG 3 cut(s) 75, 566, 834
BseNI ACTGG 1 cut(s) 566
BseXI GCAGC 1 cut(s) 437
BshFI GGCC 1 cut(s) 479
BsiHKAI GWGCWC 1 cut(s) 553
BsiSI CCGG 2 cut(s) 297, 654
BsmAI GTCTC 1 cut(s) 533
BsmI GAATGC 3 cut(s) 452, 671, 844
BsnI GGCC 1 cut(s) 479
Bso31I GGTCTC 1 cut(s) 533
Bsp1286I GDGCHC 1 cut(s) 553
Bsp143I GATC 1 cut(s) 846
Bsp1720I GCTNAGC 1 cut(s) 61
BspACI CCGC 2 cut(s) 111, 135
BspANI GGCC 1 cut(s) 479
BspCNI CTCAG 3 cut(s) 74, 565, 835
BspTNI GGTCTC 1 cut(s) 533
BsrFI RCCGGY 1 cut(s) 653
BsrI ACTGG 1 cut(s) 566
BssAI RCCGGY 1 cut(s) 653
BssMI GATC 1 cut(s) 846
BssSI CACGAG 1 cut(s) 429
Bst2BI CACGAG 1 cut(s) 429
Bst2UI CCWGG 2 cut(s) 481, 862
Bst4CI ACNGT 1 cut(s) 520
Bst6I CTCTTC 1 cut(s) 325
BstC8I GCNNGC 2 cut(s) 184, 188
BstDEI CTNAG 4 cut(s) 56, 61, 552, 843
BstF5I GGATG 3 cut(s) 184, 628, 667
BstHHI GCGC 1 cut(s) 198
BstKTI GATC 1 cut(s) 849
BstMAI GTCTC 1 cut(s) 533
BstMBI GATC 1 cut(s) 846
BstMWI GCNNNNNNNGC 4 cut(s) 119, 293, 548, 807
BstNI CCWGG 2 cut(s) 481, 862
BstNSI RCATGY 1 cut(s) 428
BstSCI CCNGG 2 cut(s) 479, 860
BstV1I GCAGC 1 cut(s) 437
BstXI CCANNNNNNTGG 2 cut(s) 86, 619
BsuRI GGCC 1 cut(s) 479
BtsCI GGATG 3 cut(s) 184, 628, 667
BtsIMutI CAGTG 1 cut(s) 672
Cac8I GCNNGC 2 cut(s) 184, 188
CfoI GCGC 1 cut(s) 198
Cfr10I RCCGGY 1 cut(s) 653
CsiI ACCWGGT 1 cut(s) 860
Csp6I GTAC 1 cut(s) 261
CspCI CAANNNNNGTGG 2 cut(s) 747, 782
CviAII CATG 5 cut(s) 305, 349, 425, 746, 774
CviQI GTAC 1 cut(s) 261
DdeI CTNAG 4 cut(s) 56, 61, 552, 843
DpnI GATC 1 cut(s) 848
DpnII GATC 1 cut(s) 846
Eam1104I CTCTTC 1 cut(s) 325
EarI CTCTTC 1 cut(s) 325
Eco147I AGGCCT 1 cut(s) 479
Eco31I GGTCTC 1 cut(s) 533
Eco57I CTGAAG 2 cut(s) 26, 696
EcoRI GAATTC 1 cut(s) 90
EcoRII CCWGG 2 cut(s) 479, 860
FaeI CATG 5 cut(s) 308, 352, 428, 749, 777
FalI AAGNNNNNCTT 2 cut(s) 223, 255
FatI CATG 5 cut(s) 304, 348, 424, 745, 773
FauI CCCGC 1 cut(s) 118
Fnu4HI GCNGC 2 cut(s) 136, 451
FokI GGATG 3 cut(s) 191, 635, 674
Fsp4HI GCNGC 2 cut(s) 136, 451
GlaI GCGC 1 cut(s) 197
GluI GCNGC 2 cut(s) 136, 451
GsuI CTGGAG 1 cut(s) 583
HaeIII GGCC 1 cut(s) 479
HapII CCGG 2 cut(s) 297, 654
HhaI GCGC 1 cut(s) 198
Hin1II CATG 5 cut(s) 308, 352, 428, 749, 777
Hin6I GCGC 1 cut(s) 196
HinP1I GCGC 1 cut(s) 196
HinfI GANTC 3 cut(s) 21, 713, 770
HpaII CCGG 2 cut(s) 297, 654
HphI GGTGA 2 cut(s) 266, 419
Hpy166II GTNNAC 2 cut(s) 319, 346
Hpy188I TCNGA 3 cut(s) 142, 555, 718
Hpy188III TCNNGA 2 cut(s) 87, 850
Hpy8I GTNNAC 2 cut(s) 319, 346
HpyAV CCTTC 2 cut(s) 116, 434
HpyCH4III ACNGT 1 cut(s) 520
HpyCH4V TGCA 3 cut(s) 280, 453, 810
HpyF10VI GCNNNNNNNGC 4 cut(s) 119, 293, 548, 807
HpyF3I CTNAG 4 cut(s) 56, 61, 552, 843
Hsp92II CATG 5 cut(s) 308, 352, 428, 749, 777
HspAI GCGC 1 cut(s) 196
Kzo9I GATC 1 cut(s) 846
LmnI GCTCC 2 cut(s) 152, 564
Lsp1109I GCAGC 1 cut(s) 437
LweI GCATC 4 cut(s) 169, 410, 462, 613
MabI ACCWGGT 1 cut(s) 860
MaeIII GTNAC 1 cut(s) 370
MalI GATC 1 cut(s) 848
MboI GATC 1 cut(s) 846
MboII GAAGA 6 cut(s) 32, 342, 448, 449, 594, 689
MhlI GDGCHC 1 cut(s) 553
MluCI AATT 8 cut(s) 90, 338, 375, 703, 720, 749, 831, 871
MmeI TCCRAC 2 cut(s) 76, 103
MnlI CCTC 4 cut(s) 201, 245, 262, 554
MseI TTAA 1 cut(s) 726
MslI CAYNNNNRTG 1 cut(s) 429
MspI CCGG 2 cut(s) 297, 654
MspR9I CCNGG 2 cut(s) 481, 862
Mva1269I GAATGC 3 cut(s) 452, 671, 844
MvaI CCWGG 2 cut(s) 481, 862
MwoI GCNNNNNNNGC 4 cut(s) 119, 293, 548, 807
NdeII GATC 1 cut(s) 846
NlaIII CATG 5 cut(s) 308, 352, 428, 749, 777
NspI RCATGY 1 cut(s) 428
PceI AGGCCT 1 cut(s) 479
PctI GAATGC 3 cut(s) 452, 671, 844
PfeI GAWTC 3 cut(s) 21, 713, 770
PkrI GCNGC 2 cut(s) 137, 452
Psp6I CCWGG 2 cut(s) 479, 860
PspGI CCWGG 2 cut(s) 479, 860
RsaI GTAC 1 cut(s) 262
RsaNI GTAC 1 cut(s) 261
RseI CAYNNNNRTG 1 cut(s) 429
SaqAI TTAA 1 cut(s) 726
SatI GCNGC 2 cut(s) 136, 451
Sau3AI GATC 1 cut(s) 846
ScrFI CCNGG 2 cut(s) 481, 862
SduI GDGCHC 1 cut(s) 553
SexAI ACCWGGT 1 cut(s) 860
SfaNI GCATC 4 cut(s) 169, 410, 462, 613
SmiMI CAYNNNNRTG 1 cut(s) 429
SmlI CTYRAG 2 cut(s) 576, 698
SmoI CTYRAG 2 cut(s) 576, 698
Sse9I AATT 8 cut(s) 90, 338, 375, 703, 720, 749, 831, 871
SseBI AGGCCT 1 cut(s) 479
SsiI CCGC 2 cut(s) 111, 135
StuI AGGCCT 1 cut(s) 479
StyD4I CCNGG 2 cut(s) 479, 860
TaaI ACNGT 1 cut(s) 520
TasI AATT 8 cut(s) 90, 338, 375, 703, 720, 749, 831, 871
TatI WGTACW 1 cut(s) 260
TauI GCSGC 1 cut(s) 138
TfiI GAWTC 3 cut(s) 21, 713, 770
Tru1I TTAA 1 cut(s) 726
Tru9I TTAA 1 cut(s) 726
TscAI CASTG 1 cut(s) 679
TseI GCWGC 1 cut(s) 450
TspDTI ATGAA 8 cut(s) 231, 293, 486, 493, 678, 743, 762, 762
TspRI CASTG 1 cut(s) 679
XapI RAATTY 3 cut(s) 90, 720, 749
XceI RCATGY 1 cut(s) 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.