Rh2DG522500

PA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0008527
Physical Location & Seq
Forward (+)
74793215 .. 74796630
3416 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG522500.1

Sequence Viewer

Length: 834 bp
ATGGAGTCTTCCCCTCTTCTGAATTTAGTTCAGTCTTCCTCTCGTTCTGAATTTGCTTCCATTAATCTTCCTCAGGAAACAGACCTTCGTTTGGATTCTACTGCACAAGCTGTTGCTGTTGATAGAGCTGGTGCTTGTGGAGCTTTGGTAAACGCAGACCCTTTGAATGCACGTTCGCCTCTACGGAATGGGCGCCAAGCAAATGAAACTGATAGAGCAAGGTTTGCTTTGATAGTTAGAGGGGAATCTGCTTTCAAGGATAAAGTACGGAATGCTCAAAATGCTGGTTTTGGTGCCGCAATTGTTTATGATGATAGAGATGGAGAAGATCTAGTTTACAGTGAACTACTGAAGGATCATGCTTATGATGAGGAAGGGGAATGCTGTATCTTTCCATTTCAAACTGAAACAGCCTGGACTGTGCTAGCTATATCTTTTATCTCATTCATTGTCATATTGGGTATATTATTAATAGCCATATTTGCTCCAAGACACTGGCTGTACTCACAAGGAAGAAACCAACTTCCTAAAAGTGTGGATAGGAAGGTGGTGGAAGCTCTGCCTTGCTTCACATTCAGTTCTGCTCGTTTGGGTGAATGCCACAGTGAAGAAACTTGCGCCATCTGCCTTGAGAATTACAAAGATGGGGAAATTCTTAAAGTTCTTCCCTGTCAACACGAATTTCATTCAAGCTGTGTGGACTCATGGCTGACAAAAAGGGGGACATGCTGCCCCGTGTGCAAGCATGATATGAGAACAAAATATGTGAATCCTGAGGTAAGAGAGTGGGGTGCATCCTCATTATTGAGTGTTCCACCGTTGAGTCTTGTTTAA

Protein Analysis

277

Amino Acids

30.69

Weight (kDa)

5.33

Isoelectric Point (pI)

45.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PA PF02225 70 - 114 4.2e-08 PA domain
zf-rbx1 PF12678 201 - 248 1.1e-06 RING-H2 zinc finger domain
zf-RING_2 PF13639 205 - 248 1.2e-12 Ring finger domain
zf-C3HC4 PF00097 206 - 247 1e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 206 - 233 1.5e-09 RING-like zinc finger
zf-C3HC4_2 PF13923 206 - 247 2.1e-08 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 192, 293
AciI CCGC 1 cut(s) 297
AclWI GGATC 1 cut(s) 363
AcsI RAATTY 4 cut(s) 22, 50, 651, 680
AcuI CTGAAG 1 cut(s) 371
AcyI GRCGYC 1 cut(s) 193
AfaI GTAC 2 cut(s) 267, 503
AfiI CCNNNNNNNGG 1 cut(s) 91
AgsI TTSAA 4 cut(s) 166, 256, 401, 690
AjnI CCWGG 1 cut(s) 413
AluBI AGCT 6 cut(s) 110, 128, 143, 428, 557, 693
AluI AGCT 6 cut(s) 110, 128, 143, 428, 557, 693
AlwI GGATC 1 cut(s) 363
ApeKI GCWGC 1 cut(s) 729
ApoI RAATTY 4 cut(s) 22, 50, 651, 680
AseI ATTAAT 2 cut(s) 63, 470
AspLEI GCGC 2 cut(s) 195, 620
AsuHPI GGTGA 1 cut(s) 605
AsuNHI GCTAGC 1 cut(s) 424
AxyI CCTNAGG 2 cut(s) 72, 774
BanI GGYRCC 2 cut(s) 192, 293
BbsI GAAGAC 1 cut(s) 27
BbvI GCAGC 1 cut(s) 716
BccI CCATC 3 cut(s) 314, 629, 638
BciT130I CCWGG 1 cut(s) 415
BfaI CTAG 2 cut(s) 332, 425
BfoI RGCGCY 1 cut(s) 196
BglII AGATCT 1 cut(s) 328
BisI GCNGC 2 cut(s) 297, 730
BlsI GCNGC 2 cut(s) 298, 731
Bme1390I CCNGG 1 cut(s) 415
BmiI GGNNCC 2 cut(s) 194, 295
BmrFI CCNGG 1 cut(s) 415
BmsI GCATC 1 cut(s) 803
BmtI GCTAGC 1 cut(s) 428
BpiI GAAGAC 1 cut(s) 27
BpuEI CTTGAG 1 cut(s) 650
BsaHI GRCGYC 1 cut(s) 193
BsaXI ACNNNNNCTCC 2 cut(s) 132, 162
Bsc4I CCNNNNNNNGG 1 cut(s) 91
Bse1I ACTGG 1 cut(s) 500
Bse21I CCTNAGG 2 cut(s) 72, 774
BseBI CCWGG 1 cut(s) 415
BseGI GGATG 1 cut(s) 794
BseLI CCNNNNNNNGG 1 cut(s) 91
BseMII CTCAG 2 cut(s) 86, 765
BseNI ACTGG 1 cut(s) 500
BseXI GCAGC 1 cut(s) 716
BsgI GTGCAG 1 cut(s) 87
BshNI GGYRCC 2 cut(s) 192, 293
BslFI GGGAC 1 cut(s) 736
BslI CCNNNNNNNGG 1 cut(s) 91
BsmFI GGGAC 1 cut(s) 736
BsmI GAATGC 4 cut(s) 172, 277, 386, 602
Bsp143I GATC 2 cut(s) 328, 355
BspACI CCGC 1 cut(s) 297
BspCNI CTCAG 2 cut(s) 85, 766
BspLI GGNNCC 2 cut(s) 194, 295
BspOI GCTAGC 1 cut(s) 428
BspPI GGATC 1 cut(s) 363
BspT107I GGYRCC 2 cut(s) 192, 293
BsrI ACTGG 1 cut(s) 500
BssMI GATC 2 cut(s) 328, 355
BssNI GRCGYC 1 cut(s) 193
Bst2UI CCWGG 1 cut(s) 415
Bst4CI ACNGT 4 cut(s) 341, 421, 605, 819
Bst6I CTCTTC 1 cut(s) 21
BstACI GRCGYC 1 cut(s) 193
BstAPI GCANNNNNTGC 1 cut(s) 224
BstC8I GCNNGC 2 cut(s) 426, 743
BstDEI CTNAG 2 cut(s) 72, 774
BstF5I GGATG 1 cut(s) 794
BstH2I RGCGCY 1 cut(s) 196
BstHHI GCGC 2 cut(s) 195, 620
BstKTI GATC 2 cut(s) 331, 358
BstMBI GATC 2 cut(s) 328, 355
BstMWI GCNNNNNNNGC 6 cut(s) 140, 224, 281, 482, 624, 738
BstNI CCWGG 1 cut(s) 415
BstNSI RCATGY 1 cut(s) 729
BstSCI CCNGG 1 cut(s) 413
BstV1I GCAGC 1 cut(s) 716
BstV2I GAAGAC 1 cut(s) 27
BstX2I RGATCY 1 cut(s) 328
BstXI CCANNNNNNTGG 1 cut(s) 495
BstYI RGATCY 1 cut(s) 328
Bsu36I CCTNAGG 2 cut(s) 72, 774
BtsCI GGATG 1 cut(s) 794
BtsIMutI CAGTG 3 cut(s) 346, 493, 610
Cac8I GCNNGC 2 cut(s) 426, 743
CfoI GCGC 2 cut(s) 195, 620
Csp6I GTAC 2 cut(s) 266, 502
CspCI CAANNNNNGTGG 2 cut(s) 678, 713
CviAII CATG 4 cut(s) 359, 705, 726, 746
CviQI GTAC 2 cut(s) 266, 502
DdeI CTNAG 2 cut(s) 72, 774
DinI GGCGCC 1 cut(s) 194
DpnI GATC 2 cut(s) 330, 357
DpnII GATC 2 cut(s) 328, 355
Eam1104I CTCTTC 1 cut(s) 21
EarI CTCTTC 1 cut(s) 21
Eco57I CTGAAG 1 cut(s) 371
Eco81I CCTNAGG 2 cut(s) 72, 774
EcoRII CCWGG 1 cut(s) 413
EgeI GGCGCC 1 cut(s) 194
EheI GGCGCC 1 cut(s) 194
FaeI CATG 4 cut(s) 362, 708, 729, 749
FalI AAGNNNNNCTT 2 cut(s) 211, 243
FaqI GGGAC 1 cut(s) 736
FatI CATG 4 cut(s) 358, 704, 725, 745
Fnu4HI GCNGC 2 cut(s) 297, 730
FokI GGATG 1 cut(s) 781
Fsp4HI GCNGC 2 cut(s) 297, 730
FspBI CTAG 2 cut(s) 332, 425
GlaI GCGC 2 cut(s) 194, 619
GluI GCNGC 2 cut(s) 297, 730
HaeII RGCGCY 1 cut(s) 196
HhaI GCGC 2 cut(s) 195, 620
Hin1I GRCGYC 1 cut(s) 193
Hin1II CATG 4 cut(s) 362, 708, 729, 749
Hin6I GCGC 2 cut(s) 193, 618
HinP1I GCGC 2 cut(s) 193, 618
HincII GTYRAC 1 cut(s) 674
HindII GTYRAC 1 cut(s) 674
HinfI GANTC 6 cut(s) 5, 95, 245, 701, 769, 823
HphI GGTGA 1 cut(s) 605
Hpy166II GTNNAC 5 cut(s) 151, 337, 344, 674, 700
Hpy188I TCNGA 2 cut(s) 21, 49
Hpy188III TCNNGA 2 cut(s) 74, 773
Hpy8I GTNNAC 5 cut(s) 151, 337, 344, 674, 700
HpyAV CCTTC 4 cut(s) 95, 346, 368, 538
HpyCH4III ACNGT 4 cut(s) 341, 421, 605, 819
HpyCH4IV ACGT 1 cut(s) 172
HpyCH4V TGCA 4 cut(s) 104, 170, 741, 794
HpyF10VI GCNNNNNNNGC 6 cut(s) 140, 224, 281, 482, 624, 738
HpyF3I CTNAG 2 cut(s) 72, 774
HpySE526I ACGT 1 cut(s) 172
Hsp92I GRCGYC 1 cut(s) 193
Hsp92II CATG 4 cut(s) 362, 708, 729, 749
HspAI GCGC 2 cut(s) 193, 618
KasI GGCGCC 1 cut(s) 192
Kzo9I GATC 2 cut(s) 328, 355
LmnI GCTCC 2 cut(s) 140, 490
LpnPI CCDG 8 cut(s) 59, 114, 270, 400, 427, 481, 682, 786
Lsp1109I GCAGC 1 cut(s) 716
LweI GCATC 1 cut(s) 803
MaeI CTAG 2 cut(s) 332, 425
MaeII ACGT 1 cut(s) 172
MalI GATC 2 cut(s) 330, 357
MboI GATC 2 cut(s) 328, 355
MboII GAAGA 7 cut(s) 8, 27, 59, 338, 525, 620, 656
MfeI CAATTG 1 cut(s) 300
MflI RGATCY 1 cut(s) 328
MluCI AATT 6 cut(s) 22, 50, 300, 634, 651, 680
Mly113I GGCGCC 1 cut(s) 193
MlyI GAGTC 3 cut(s) 14, 695, 832
MnlI CCTC 8 cut(s) 24, 49, 81, 189, 233, 364, 769, 808
MseI TTAA 4 cut(s) 63, 470, 657, 832
MslI CAYNNNNRTG 1 cut(s) 363
MspR9I CCNGG 1 cut(s) 415
MunI CAATTG 1 cut(s) 300
Mva1269I GAATGC 4 cut(s) 172, 277, 386, 602
MvaI CCWGG 1 cut(s) 415
MwoI GCNNNNNNNGC 6 cut(s) 140, 224, 281, 482, 624, 738
NarI GGCGCC 1 cut(s) 193
NdeII GATC 2 cut(s) 328, 355
NheI GCTAGC 1 cut(s) 424
NlaIII CATG 4 cut(s) 362, 708, 729, 749
NlaIV GGNNCC 2 cut(s) 194, 295
NspI RCATGY 1 cut(s) 729
PctI GAATGC 4 cut(s) 172, 277, 386, 602
PfeI GAWTC 3 cut(s) 95, 245, 769
PkrI GCNGC 2 cut(s) 298, 731
PleI GAGTC 3 cut(s) 13, 695, 831
PluTI GGCGCC 1 cut(s) 196
PpsI GAGTC 3 cut(s) 13, 695, 831
PshBI ATTAAT 2 cut(s) 63, 470
Psp6I CCWGG 1 cut(s) 413
PspGI CCWGG 1 cut(s) 413
PspN4I GGNNCC 2 cut(s) 194, 295
PsuI RGATCY 1 cut(s) 328
RsaI GTAC 2 cut(s) 267, 503
RsaNI GTAC 2 cut(s) 266, 502
RseI CAYNNNNRTG 1 cut(s) 363
SaqAI TTAA 4 cut(s) 63, 470, 657, 832
SatI GCNGC 2 cut(s) 297, 730
Sau3AI GATC 2 cut(s) 328, 355
SchI GAGTC 3 cut(s) 14, 695, 832
ScrFI CCNGG 1 cut(s) 415
SfaNI GCATC 1 cut(s) 803
SfoI GGCGCC 1 cut(s) 194
SmiMI CAYNNNNRTG 1 cut(s) 363
SmlI CTYRAG 1 cut(s) 629
SmoI CTYRAG 1 cut(s) 629
Sse9I AATT 6 cut(s) 22, 50, 300, 634, 651, 680
SsiI CCGC 1 cut(s) 297
SspDI GGCGCC 1 cut(s) 192
SspMI CTAG 2 cut(s) 332, 425
StyD4I CCNGG 1 cut(s) 413
TaaI ACNGT 4 cut(s) 341, 421, 605, 819
TaiI ACGT 1 cut(s) 175
TasI AATT 6 cut(s) 22, 50, 300, 634, 651, 680
TatI WGTACW 1 cut(s) 501
TauI GCSGC 1 cut(s) 299
TfiI GAWTC 3 cut(s) 95, 245, 769
Tru1I TTAA 4 cut(s) 63, 470, 657, 832
Tru9I TTAA 4 cut(s) 63, 470, 657, 832
TscAI CASTG 3 cut(s) 346, 500, 610
TseI GCWGC 1 cut(s) 729
TspDTI ATGAA 3 cut(s) 219, 436, 674
TspGWI ACGGA 2 cut(s) 199, 283
TspRI CASTG 3 cut(s) 346, 500, 610
VspI ATTAAT 2 cut(s) 63, 470
XapI RAATTY 4 cut(s) 22, 50, 651, 680
XceI RCATGY 1 cut(s) 729
XspI CTAG 2 cut(s) 332, 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.