Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0008527

Physical Location & Seq

Genomic Coordinates Reverse (-)

20031731 .. 20035005

Gene Structure 3275 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1243900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 879 bp

ATGGGCCAAGCTGTAGTGGGATTCTCTATGATTGCTTGTTTTTTCCTTCTCCAACTAAGCTCTGCTAATGTTGTTCTCCAACCATCTGGAATTCTTGTCGCCGACCTTCCCGCCAAATTTGCTGGTCCTGTCAGTAGCTCTGAAATATGTGGAGTTTTGCTTGTATCCGACCCTTTGCATGCCTGCTCGCCTCTACGCAATGGTATTCAAGCAAGTGAAACTGATATAACGAGGTTTGCTTTAGTAGAAAGAGGTGAATGCACTTTCGAGGATAAAGTGCAACACGCTCAAGATGCCGGATTTCATGCTGTGATTGTTTATGATGATAGAAAAGAAAAATTGGTTCACATGATGATAAACAGCAAGAATGTTACAATTCGTTCTGTATTTGTTTCGAAAGCATCTGGTGAAATGCTAAAGGAGCATGCTCGTGGAGAAGAAGGGGAATGCTGCATCTTCCTATTGCCAAATGAAAAGGCCTGGTTGGTGTTTGCTGTATCTTTGATCTCATTTCTAACCGTATTGGGTCTCCTTATAATTGCCAACTGTGCTCGGAGAGACTGGCGCTGGCCCTATTCTCAAGGAAGAAACCAGCTTCCTAAAAGTGCGGATACCAAGATTGTGGATGCTCTCCCTTGCTTCACATTCAGTTCTGCTGGTTTGGATGAATGCCACACTGAAGAAACTTGTGCCATCTGCCTTGAGAACTACAAAGATTCCGAAATTCTTAAAGTTCTTCCCTGCCAGCATGAATTTCATTCAAGTTGTGTGGATTCATGGCTGAGAAAATGGGGAAGAAACTGCCCAGTGTGCAAGCATGATATACGAAAGAAAATTGCGACTGCTGAGATCAAGAGAAAATCCTGGTTATACAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000306 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005783 GO:0006810 GO:0006886 GO:0008104 GO:0008150 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0019898 GO:0031090 GO:0031312 GO:0033036 GO:0034613 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043621 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070727 GO:0071702 GO:0071705 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PA Rhodanese zf-RING_11 zf-RING_2

Protein Analysis

293

Amino Acids

32.55

Weight (kDa)

6.74

Isoelectric Point (pI)

47.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PA	PF02225	77 - 139	2.5e-13	PA domain
zf-rbx1	PF12678	224 - 272	7.3e-08	RING-H2 zinc finger domain
zf-RING_2	PF13639	229 - 272	2e-12	Ring finger domain
zf-C3HC4	PF00097	230 - 271	6.1e-11	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2	PF13923	230 - 271	1.6e-09	Zinc finger, C3HC4 type (RING finger)
zf-RING_11	PF17123	230 - 257	5.9e-09	RING-like zinc finger
zf-RING_UBOX	PF13445	230 - 269	8.4e-07	RING-type zinc-finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008527)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G66160 AT5G66160
fragaria_vesca	FvH4_1g11650 FvH4_1g11650
malus_domestica	MD02G1129600.v1.1 MD15G1243900.v1.1
prunus_persica	Prupe.7G170000_v2.0.a1 Prupe.7G170000_v2.0.a1
pyrus_communis	pycom02g10150 pycom15g21480
rosa_chinensis	RchiOBHm_Chr2g0099241
rosa_laevigata	RLG00000016864
rosa_multiflora	Rmu_sc0002025.1_g000005
rosa_roxburghii	Rroxscaffold_2G00143290
rosa_rugosa	Rorug02G0078100
rosa_samantha	Rh2AG127300 Rh2BG130300 Rh2BG510800 Rh2CG131500 Rh2DG131900 Rh2DG522500 Rh5AG471500
rosa_wichuraiana	Rw2G009820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	536
AciI	CCGC	2 cut(s)	111, 608
AcsI	RAATTY	4 cut(s)	90, 116, 723, 752
AcuI	CTGAAG	1 cut(s)	699
AgsI	TTSAA	2 cut(s)	209, 762
AjnI	CCWGG	2 cut(s)	479, 863
AluBI	AGCT	4 cut(s)	11, 60, 138, 595
AluI	AGCT	4 cut(s)	11, 60, 138, 595
Alw21I	GWGCWC	1 cut(s)	553
Alw26I	GTCTC	2 cut(s)	533, 552
AoxI	GGCC	3 cut(s)	4, 477, 569
ApeKI	GCWGC	1 cut(s)	450
ApoI	RAATTY	4 cut(s)	90, 116, 723, 752
AspLEI	GCGC	1 cut(s)	567
AspS9I	GGNCC	3 cut(s)	4, 125, 570
AsuHPI	GGTGA	2 cut(s)	266, 419
AsuII	TTCGAA	1 cut(s)	395
AvaII	GGWCC	1 cut(s)	125
BauI	CACGAG	1 cut(s)	429
Bbv12I	GWGCWC	1 cut(s)	553
BbvI	GCAGC	1 cut(s)	437
BccI	CCATC	2 cut(s)	91, 701
BciT130I	CCWGG	2 cut(s)	481, 865
BciVI	GTATCC	2 cut(s)	175, 604
BcoDI	GTCTC	2 cut(s)	533, 552
BfmI	CTRYAG	1 cut(s)	12
BfoI	RGCGCY	1 cut(s)	568
BfuI	GTATCC	2 cut(s)	175, 604
BisI	GCNGC	1 cut(s)	451
BlsI	GCNGC	1 cut(s)	452
Bme1390I	CCNGG	2 cut(s)	481, 865
Bme18I	GGWCC	1 cut(s)	125
BmgT120I	GGNCC	3 cut(s)	4, 125, 570
BmrFI	CCNGG	2 cut(s)	481, 865
BmrI	ACTGGG	1 cut(s)	800
BmsI	GCATC	4 cut(s)	283, 410, 462, 616
BmuI	ACTGGG	1 cut(s)	800
Bpu14I	TTCGAA	1 cut(s)	395
BpuEI	CTTGAG	3 cut(s)	273, 564, 722
BsaI	GGTCTC	1 cut(s)	533
Bse1I	ACTGG	2 cut(s)	566, 806
Bse3DI	GCAATG	1 cut(s)	205
BseBI	CCWGG	2 cut(s)	481, 865
BseGI	GGATG	2 cut(s)	631, 670
BseMI	GCAATG	1 cut(s)	205
BseMII	CTCAG	2 cut(s)	773, 837
BseNI	ACTGG	2 cut(s)	566, 806
BseXI	GCAGC	1 cut(s)	437
BshFI	GGCC	3 cut(s)	6, 479, 571
BsiHKAI	GWGCWC	1 cut(s)	553
BsiSI	CCGG	1 cut(s)	297
BsmAI	GTCTC	2 cut(s)	533, 552
BsmI	GAATGC	3 cut(s)	263, 452, 674
BsnI	GGCC	3 cut(s)	6, 479, 571
Bso31I	GGTCTC	1 cut(s)	533
Bsp119I	TTCGAA	1 cut(s)	395
Bsp1286I	GDGCHC	1 cut(s)	553
Bsp143I	GATC	2 cut(s)	504, 849
BspACI	CCGC	2 cut(s)	111, 608
BspANI	GGCC	3 cut(s)	6, 479, 571
BspCNI	CTCAG	2 cut(s)	774, 838
BspT104I	TTCGAA	1 cut(s)	395
BspTNI	GGTCTC	1 cut(s)	533
BsrDI	GCAATG	1 cut(s)	205
BsrI	ACTGG	2 cut(s)	566, 806
BssMI	GATC	2 cut(s)	504, 849
BssSI	CACGAG	1 cut(s)	429
Bst2BI	CACGAG	1 cut(s)	429
Bst2UI	CCWGG	2 cut(s)	481, 865
Bst4CI	ACNGT	2 cut(s)	520, 548
BstBI	TTCGAA	1 cut(s)	395
BstC8I	GCNNGC	7 cut(s)	180, 184, 188, 426, 569, 746, 815
BstDEI	CTNAG	3 cut(s)	56, 782, 846
BstF5I	GGATG	2 cut(s)	631, 670
BstH2I	RGCGCY	1 cut(s)	568
BstHHI	GCGC	1 cut(s)	567
BstKTI	GATC	2 cut(s)	507, 852
BstMAI	GTCTC	2 cut(s)	533, 552
BstMBI	GATC	2 cut(s)	504, 849
BstMWI	GCNNNNNNNGC	5 cut(s)	119, 293, 421, 548, 810
BstNI	CCWGG	2 cut(s)	481, 865
BstNSI	RCATGY	2 cut(s)	182, 428
BstSCI	CCNGG	2 cut(s)	479, 863
BstSFI	CTRYAG	1 cut(s)	12
BstV1I	GCAGC	1 cut(s)	437
BstXI	CCANNNNNNTGG	2 cut(s)	86, 622
BsuI	GTATCC	2 cut(s)	175, 604
BsuRI	GGCC	3 cut(s)	6, 479, 571
BtsCI	GGATG	2 cut(s)	631, 670
BtsIMutI	CAGTG	2 cut(s)	675, 813
Cac8I	GCNNGC	7 cut(s)	180, 184, 188, 426, 569, 746, 815
CfoI	GCGC	1 cut(s)	567
Cfr13I	GGNCC	3 cut(s)	4, 125, 570
CspCI	CAANNNNNGTGG	2 cut(s)	750, 785
CviAII	CATG	7 cut(s)	179, 305, 349, 425, 749, 777, 818
CviJI	RGCY	8 cut(s)	6, 11, 60, 138, 479, 571, 595, 781
CviKI_1	RGCY	8 cut(s)	6, 11, 60, 138, 479, 571, 595, 781
DdeI	CTNAG	3 cut(s)	56, 782, 846
DpnI	GATC	2 cut(s)	506, 851
DpnII	GATC	2 cut(s)	504, 849
Eco147I	AGGCCT	1 cut(s)	479
Eco31I	GGTCTC	1 cut(s)	533
Eco47I	GGWCC	1 cut(s)	125
Eco57I	CTGAAG	1 cut(s)	699
EcoRI	GAATTC	1 cut(s)	90
EcoRII	CCWGG	2 cut(s)	479, 863
FaeI	CATG	7 cut(s)	182, 308, 352, 428, 752, 780, 821
FatI	CATG	7 cut(s)	178, 304, 348, 424, 748, 776, 817
FauI	CCCGC	1 cut(s)	118
Fnu4HI	GCNGC	1 cut(s)	451
FokI	GGATG	2 cut(s)	638, 677
Fsp4HI	GCNGC	1 cut(s)	451
GlaI	GCGC	1 cut(s)	566
GluI	GCNGC	1 cut(s)	451
HaeII	RGCGCY	1 cut(s)	568
HaeIII	GGCC	3 cut(s)	6, 479, 571
HapII	CCGG	1 cut(s)	297
HhaI	GCGC	1 cut(s)	567
Hin1II	CATG	7 cut(s)	182, 308, 352, 428, 752, 780, 821
Hin6I	GCGC	1 cut(s)	565
HinP1I	GCGC	1 cut(s)	565
HinfI	GANTC	3 cut(s)	21, 716, 773
HpaII	CCGG	1 cut(s)	297
HphI	GGTGA	2 cut(s)	266, 419
Hpy166II	GTNNAC	1 cut(s)	346
Hpy188I	TCNGA	4 cut(s)	142, 169, 555, 721
Hpy188III	TCNNGA	3 cut(s)	87, 290, 853
Hpy8I	GTNNAC	1 cut(s)	346
HpyAV	CCTTC	3 cut(s)	56, 116, 434
HpyCH4III	ACNGT	2 cut(s)	520, 548
HpyCH4V	TGCA	5 cut(s)	178, 261, 280, 453, 813
HpyF10VI	GCNNNNNNNGC	5 cut(s)	119, 293, 421, 548, 810
HpyF3I	CTNAG	3 cut(s)	56, 782, 846
Hsp92II	CATG	7 cut(s)	182, 308, 352, 428, 752, 780, 821
HspAI	GCGC	1 cut(s)	565
Kzo9I	GATC	2 cut(s)	504, 849
LmnI	GCTCC	1 cut(s)	421
Lsp1109I	GCAGC	1 cut(s)	437
LweI	GCATC	4 cut(s)	283, 410, 462, 616
MaeIII	GTNAC	1 cut(s)	370
MalI	GATC	2 cut(s)	506, 851
MboI	GATC	2 cut(s)	504, 849
MboII	GAAGA	6 cut(s)	448, 449, 597, 692, 728, 807
MhlI	GDGCHC	1 cut(s)	553
MluCI	AATT	9 cut(s)	90, 116, 338, 375, 537, 723, 752, 834, 874
MmeI	TCCRAC	3 cut(s)	76, 103, 192
MnlI	CCTC	4 cut(s)	201, 225, 245, 262
MseI	TTAA	1 cut(s)	729
MslI	CAYNNNNRTG	1 cut(s)	429
MspI	CCGG	1 cut(s)	297
MspR9I	CCNGG	2 cut(s)	481, 865
Mva1269I	GAATGC	3 cut(s)	263, 452, 674
MvaI	CCWGG	2 cut(s)	481, 865
MwoI	GCNNNNNNNGC	5 cut(s)	119, 293, 421, 548, 810
NdeII	GATC	2 cut(s)	504, 849
NlaIII	CATG	7 cut(s)	182, 308, 352, 428, 752, 780, 821
NspI	RCATGY	2 cut(s)	182, 428
NspV	TTCGAA	1 cut(s)	395
PaeI	GCATGC	2 cut(s)	182, 428
PceI	AGGCCT	1 cut(s)	479
PctI	GAATGC	3 cut(s)	263, 452, 674
PfeI	GAWTC	3 cut(s)	21, 716, 773
PkrI	GCNGC	1 cut(s)	452
PsiI	TTATAA	1 cut(s)	536
Psp6I	CCWGG	2 cut(s)	479, 863
PspGI	CCWGG	2 cut(s)	479, 863
PspPI	GGNCC	3 cut(s)	4, 125, 570
RseI	CAYNNNNRTG	1 cut(s)	429
SaqAI	TTAA	1 cut(s)	729
SatI	GCNGC	1 cut(s)	451
Sau3AI	GATC	2 cut(s)	504, 849
Sau96I	GGNCC	3 cut(s)	4, 125, 570
ScrFI	CCNGG	2 cut(s)	481, 865
SduI	GDGCHC	1 cut(s)	553
SetI	ASST	7 cut(s)	13, 62, 108, 140, 236, 256, 597
SfaNI	GCATC	4 cut(s)	283, 410, 462, 616
SfcI	CTRYAG	1 cut(s)	12
SfuI	TTCGAA	1 cut(s)	395
SinI	GGWCC	1 cut(s)	125
SmiMI	CAYNNNNRTG	1 cut(s)	429
SmlI	CTYRAG	3 cut(s)	288, 579, 701
SmoI	CTYRAG	3 cut(s)	288, 579, 701
SphI	GCATGC	2 cut(s)	182, 428
Sse9I	AATT	9 cut(s)	90, 116, 338, 375, 537, 723, 752, 834, 874
SseBI	AGGCCT	1 cut(s)	479
SsiI	CCGC	2 cut(s)	111, 608
StuI	AGGCCT	1 cut(s)	479
StyD4I	CCNGG	2 cut(s)	479, 863
TaaI	ACNGT	2 cut(s)	520, 548
TaqI	TCGA	2 cut(s)	267, 395
TasI	AATT	9 cut(s)	90, 116, 338, 375, 537, 723, 752, 834, 874
TfiI	GAWTC	3 cut(s)	21, 716, 773
Tru1I	TTAA	1 cut(s)	729
Tru9I	TTAA	1 cut(s)	729
TscAI	CASTG	2 cut(s)	682, 813
TseI	GCWGC	1 cut(s)	450
TspDTI	ATGAA	6 cut(s)	293, 486, 681, 746, 765, 765
TspRI	CASTG	2 cut(s)	682, 813
VpaK11BI	GGWCC	1 cut(s)	125
XapI	RAATTY	4 cut(s)	90, 116, 723, 752
XceI	RCATGY	2 cut(s)	182, 428

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.