Rh2BG510800

PA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0008527
Physical Location & Seq
Forward (+)
71868812 .. 71873393
4582 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG510800.1

Sequence Viewer

Length: 882 bp
ATGTTGCATCTTCTTGTGTTGCGTAGTTTGCTCTTCTTCTTTCTACAACTGGGCCAAAACGGAAATAGAGCTTTGTTTGGACTCTACTTCACAAGGAAACAGACCTTCGTTTGGATTCTACTGCACAAGGAAATAGAGCATTGTTTGGACTCTACTTCACAAGGAAACAGACCTTCGTTTGGATTCTACTGCACAAGAGCTGGTGCTTGTGGAGCTTTGGTAAACGCAGACCCTTTGAATGCACGTTCGCCTCTACGGAATGGGCGCCAAGCAAATGAAACTGATAGAGCAAGGTTTGCTTTGATAGTTAGAGGGGAATCTGCTTTCAAGGATAAAGTACGGAATGCTCAAAATGCTGGTTTTGGTGCCGCAATTGTTTATGATGATAGAGATGGAGAAGATCTAGTTTACAGTGAACTACTGAAGGATCATGCTTATGATGAGGAAGGGGAATGCTGTATCTTTCCATTTCAAACTGAAACAGCCTGGACTGTGCTAGCTATATCTTTTATCTCATTCATTGTCATATTGGGTATATTATTAATAGCCATATTTGCTCCAAGACACTGGCTGTACTCACAAGGAAGAAACCAACTTCCTAAAAGTGTGGATAGGAAGGTGGTGGAAGCTCTGCCTTGCTTCACATTCAGTTCTGCTCGTTTGGGTGAATGCCACAGTGAAGAAACTTGCGCCATCTGCCTTGAGAATTACAAAGATGGGGAAATTCTTAAAGTTCTTCCCTGTCAACACGAATTTCATTCAAGCTGTGTGGACTCATGGCTGACAAAAAGGGGGACATGCTGCCCCGTGTGCAAGCATGATATGAGAACAAAATATGTGAATCCTGAGATCAAGAGAAGGACCTGGTTGGATGATTCATGA

Protein Analysis

293

Amino Acids

33.32

Weight (kDa)

7.07

Isoelectric Point (pI)

48.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PA PF02225 96 - 138 4e-08 PA domain
zf-rbx1 PF12678 225 - 272 1.2e-06 RING-H2 zinc finger domain
zf-RING_2 PF13639 229 - 272 1.3e-12 Ring finger domain
zf-C3HC4 PF00097 230 - 271 1.1e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 230 - 257 1.6e-09 RING-like zinc finger
zf-C3HC4_2 PF13923 230 - 271 2.3e-08 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 264, 365
AciI CCGC 1 cut(s) 369
AclWI GGATC 1 cut(s) 435
AcsI RAATTY 2 cut(s) 723, 752
AcuI CTGAAG 1 cut(s) 443
AcyI GRCGYC 1 cut(s) 265
AfaI GTAC 2 cut(s) 339, 575
AfiI CCNNNNNNNGG 2 cut(s) 111, 179
AgsI TTSAA 4 cut(s) 238, 328, 473, 762
AjnI CCWGG 2 cut(s) 485, 863
AluBI AGCT 6 cut(s) 71, 200, 215, 500, 629, 765
AluI AGCT 6 cut(s) 71, 200, 215, 500, 629, 765
AlwI GGATC 1 cut(s) 435
AoxI GGCC 1 cut(s) 52
ApeKI GCWGC 1 cut(s) 801
ApoI RAATTY 2 cut(s) 723, 752
AseI ATTAAT 1 cut(s) 542
AspLEI GCGC 2 cut(s) 267, 692
AspS9I GGNCC 2 cut(s) 52, 861
AsuHPI GGTGA 1 cut(s) 677
AsuNHI GCTAGC 1 cut(s) 496
AvaII GGWCC 1 cut(s) 861
BanI GGYRCC 2 cut(s) 264, 365
BbvI GCAGC 1 cut(s) 788
BccI CCATC 3 cut(s) 386, 701, 710
BciT130I CCWGG 2 cut(s) 487, 865
BfaI CTAG 2 cut(s) 404, 497
BfoI RGCGCY 1 cut(s) 268
BglII AGATCT 1 cut(s) 400
BisI GCNGC 2 cut(s) 369, 802
BlsI GCNGC 2 cut(s) 370, 803
Bme1390I CCNGG 2 cut(s) 487, 865
Bme18I GGWCC 1 cut(s) 861
BmgT120I GGNCC 2 cut(s) 52, 861
BmiI GGNNCC 2 cut(s) 266, 367
BmrFI CCNGG 2 cut(s) 487, 865
BmrI ACTGGG 1 cut(s) 59
BmsI GCATC 1 cut(s) 16
BmtI GCTAGC 1 cut(s) 500
BmuI ACTGGG 1 cut(s) 59
BpuEI CTTGAG 1 cut(s) 722
BsaHI GRCGYC 1 cut(s) 265
BsaXI ACNNNNNCTCC 2 cut(s) 204, 234
Bsc4I CCNNNNNNNGG 2 cut(s) 111, 179
Bse1I ACTGG 2 cut(s) 54, 572
BseBI CCWGG 2 cut(s) 487, 865
BseGI GGATG 1 cut(s) 877
BseLI CCNNNNNNNGG 2 cut(s) 111, 179
BseMII CTCAG 1 cut(s) 837
BseNI ACTGG 2 cut(s) 54, 572
BseXI GCAGC 1 cut(s) 788
BsgI GTGCAG 2 cut(s) 107, 175
BshFI GGCC 1 cut(s) 54
BshNI GGYRCC 2 cut(s) 264, 365
BslFI GGGAC 1 cut(s) 808
BslI CCNNNNNNNGG 2 cut(s) 111, 179
BsmFI GGGAC 1 cut(s) 808
BsmI GAATGC 4 cut(s) 244, 349, 458, 674
BsnI GGCC 1 cut(s) 54
Bsp143I GATC 3 cut(s) 400, 427, 849
BspACI CCGC 1 cut(s) 369
BspANI GGCC 1 cut(s) 54
BspCNI CTCAG 1 cut(s) 838
BspHI TCATGA 1 cut(s) 878
BspLI GGNNCC 2 cut(s) 266, 367
BspOI GCTAGC 1 cut(s) 500
BspPI GGATC 1 cut(s) 435
BspQI GCTCTTC 1 cut(s) 38
BspT107I GGYRCC 2 cut(s) 264, 365
BsrI ACTGG 2 cut(s) 54, 572
BssMI GATC 3 cut(s) 400, 427, 849
BssNI GRCGYC 1 cut(s) 265
Bst2UI CCWGG 2 cut(s) 487, 865
Bst4CI ACNGT 3 cut(s) 413, 493, 677
Bst6I CTCTTC 1 cut(s) 38
BstACI GRCGYC 1 cut(s) 265
BstAPI GCANNNNNTGC 1 cut(s) 296
BstC8I GCNNGC 2 cut(s) 498, 815
BstDEI CTNAG 1 cut(s) 846
BstF5I GGATG 1 cut(s) 877
BstH2I RGCGCY 1 cut(s) 268
BstHHI GCGC 2 cut(s) 267, 692
BstKTI GATC 3 cut(s) 403, 430, 852
BstMBI GATC 3 cut(s) 400, 427, 849
BstMWI GCNNNNNNNGC 7 cut(s) 28, 212, 296, 353, 554, 696, 810
BstNI CCWGG 2 cut(s) 487, 865
BstNSI RCATGY 1 cut(s) 801
BstSCI CCNGG 2 cut(s) 485, 863
BstV1I GCAGC 1 cut(s) 788
BstX2I RGATCY 1 cut(s) 400
BstXI CCANNNNNNTGG 1 cut(s) 567
BstYI RGATCY 1 cut(s) 400
BsuRI GGCC 1 cut(s) 54
BtsCI GGATG 1 cut(s) 877
BtsIMutI CAGTG 3 cut(s) 418, 565, 682
Cac8I GCNNGC 2 cut(s) 498, 815
CciI TCATGA 1 cut(s) 878
CfoI GCGC 2 cut(s) 267, 692
Cfr13I GGNCC 2 cut(s) 52, 861
CsiI ACCWGGT 1 cut(s) 863
Csp6I GTAC 2 cut(s) 338, 574
CspCI CAANNNNNGTGG 2 cut(s) 750, 785
CviAII CATG 5 cut(s) 431, 777, 798, 818, 879
CviQI GTAC 2 cut(s) 338, 574
DdeI CTNAG 1 cut(s) 846
DinI GGCGCC 1 cut(s) 266
DpnI GATC 3 cut(s) 402, 429, 851
DpnII GATC 3 cut(s) 400, 427, 849
Eam1104I CTCTTC 1 cut(s) 38
EarI CTCTTC 1 cut(s) 38
Eco47I GGWCC 1 cut(s) 861
Eco57I CTGAAG 1 cut(s) 443
EcoO109I RGGNCCY 1 cut(s) 861
EcoRII CCWGG 2 cut(s) 485, 863
EgeI GGCGCC 1 cut(s) 266
EheI GGCGCC 1 cut(s) 266
FaeI CATG 5 cut(s) 434, 780, 801, 821, 882
FalI AAGNNNNNCTT 2 cut(s) 283, 315
FaqI GGGAC 1 cut(s) 808
FatI CATG 5 cut(s) 430, 776, 797, 817, 878
Fnu4HI GCNGC 2 cut(s) 369, 802
Fsp4HI GCNGC 2 cut(s) 369, 802
FspBI CTAG 2 cut(s) 404, 497
GlaI GCGC 2 cut(s) 266, 691
GluI GCNGC 2 cut(s) 369, 802
HaeII RGCGCY 1 cut(s) 268
HaeIII GGCC 1 cut(s) 54
HhaI GCGC 2 cut(s) 267, 692
Hin1I GRCGYC 1 cut(s) 265
Hin1II CATG 5 cut(s) 434, 780, 801, 821, 882
Hin6I GCGC 2 cut(s) 265, 690
HinP1I GCGC 2 cut(s) 265, 690
HincII GTYRAC 1 cut(s) 746
HindII GTYRAC 1 cut(s) 746
HinfI GANTC 8 cut(s) 81, 115, 149, 183, 317, 773, 841, 875
HphI GGTGA 1 cut(s) 677
Hpy166II GTNNAC 5 cut(s) 223, 409, 416, 746, 772
Hpy188III TCNNGA 3 cut(s) 845, 853, 879
Hpy8I GTNNAC 5 cut(s) 223, 409, 416, 746, 772
HpyAV CCTTC 6 cut(s) 115, 183, 418, 440, 610, 852
HpyCH4III ACNGT 3 cut(s) 413, 493, 677
HpyCH4IV ACGT 1 cut(s) 244
HpyCH4V TGCA 5 cut(s) 7, 124, 192, 242, 813
HpyF10VI GCNNNNNNNGC 7 cut(s) 28, 212, 296, 353, 554, 696, 810
HpyF3I CTNAG 1 cut(s) 846
HpySE526I ACGT 1 cut(s) 244
Hsp92I GRCGYC 1 cut(s) 265
Hsp92II CATG 5 cut(s) 434, 780, 801, 821, 882
HspAI GCGC 2 cut(s) 265, 690
KasI GGCGCC 1 cut(s) 264
Kzo9I GATC 3 cut(s) 400, 427, 849
LguI GCTCTTC 1 cut(s) 38
LmnI GCTCC 2 cut(s) 212, 562
Lsp1109I GCAGC 1 cut(s) 788
LweI GCATC 1 cut(s) 16
MabI ACCWGGT 1 cut(s) 863
MaeI CTAG 2 cut(s) 404, 497
MaeII ACGT 1 cut(s) 244
MalI GATC 3 cut(s) 402, 429, 851
MboI GATC 3 cut(s) 400, 427, 849
MboII GAAGA 6 cut(s) 25, 28, 410, 597, 692, 728
MfeI CAATTG 1 cut(s) 372
MflI RGATCY 1 cut(s) 400
MluCI AATT 4 cut(s) 372, 706, 723, 752
Mly113I GGCGCC 1 cut(s) 265
MlyI GAGTC 3 cut(s) 75, 143, 767
MmeI TCCRAC 1 cut(s) 849
MnlI CCTC 3 cut(s) 261, 305, 436
MseI TTAA 2 cut(s) 542, 729
MslI CAYNNNNRTG 1 cut(s) 435
MspR9I CCNGG 2 cut(s) 487, 865
MunI CAATTG 1 cut(s) 372
Mva1269I GAATGC 4 cut(s) 244, 349, 458, 674
MvaI CCWGG 2 cut(s) 487, 865
MwoI GCNNNNNNNGC 7 cut(s) 28, 212, 296, 353, 554, 696, 810
NarI GGCGCC 1 cut(s) 265
NdeII GATC 3 cut(s) 400, 427, 849
NheI GCTAGC 1 cut(s) 496
NlaIII CATG 5 cut(s) 434, 780, 801, 821, 882
NlaIV GGNNCC 2 cut(s) 266, 367
NspI RCATGY 1 cut(s) 801
PagI TCATGA 1 cut(s) 878
PciSI GCTCTTC 1 cut(s) 38
PctI GAATGC 4 cut(s) 244, 349, 458, 674
PfeI GAWTC 5 cut(s) 115, 183, 317, 841, 875
PkrI GCNGC 2 cut(s) 370, 803
PleI GAGTC 3 cut(s) 75, 143, 767
PluTI GGCGCC 1 cut(s) 268
PpsI GAGTC 3 cut(s) 75, 143, 767
PpuMI RGGWCCY 1 cut(s) 861
PshBI ATTAAT 1 cut(s) 542
Psp5II RGGWCCY 1 cut(s) 861
Psp6I CCWGG 2 cut(s) 485, 863
PspGI CCWGG 2 cut(s) 485, 863
PspN4I GGNNCC 2 cut(s) 266, 367
PspPI GGNCC 2 cut(s) 52, 861
PspPPI RGGWCCY 1 cut(s) 861
PsuI RGATCY 1 cut(s) 400
RsaI GTAC 2 cut(s) 339, 575
RsaNI GTAC 2 cut(s) 338, 574
RseI CAYNNNNRTG 1 cut(s) 435
SapI GCTCTTC 1 cut(s) 38
SaqAI TTAA 2 cut(s) 542, 729
SatI GCNGC 2 cut(s) 369, 802
Sau3AI GATC 3 cut(s) 400, 427, 849
Sau96I GGNCC 2 cut(s) 52, 861
SchI GAGTC 3 cut(s) 75, 143, 767
ScrFI CCNGG 2 cut(s) 487, 865
SexAI ACCWGGT 1 cut(s) 863
SfaNI GCATC 1 cut(s) 16
SfoI GGCGCC 1 cut(s) 266
SinI GGWCC 1 cut(s) 861
SmiMI CAYNNNNRTG 1 cut(s) 435
SmlI CTYRAG 1 cut(s) 701
SmoI CTYRAG 1 cut(s) 701
Sse9I AATT 4 cut(s) 372, 706, 723, 752
SsiI CCGC 1 cut(s) 369
SspDI GGCGCC 1 cut(s) 264
SspMI CTAG 2 cut(s) 404, 497
StyD4I CCNGG 2 cut(s) 485, 863
TaaI ACNGT 3 cut(s) 413, 493, 677
TaiI ACGT 1 cut(s) 247
TasI AATT 4 cut(s) 372, 706, 723, 752
TatI WGTACW 1 cut(s) 573
TauI GCSGC 1 cut(s) 371
TfiI GAWTC 5 cut(s) 115, 183, 317, 841, 875
Tru1I TTAA 2 cut(s) 542, 729
Tru9I TTAA 2 cut(s) 542, 729
TscAI CASTG 3 cut(s) 418, 572, 682
TseI GCWGC 1 cut(s) 801
TspDTI ATGAA 4 cut(s) 291, 508, 746, 867
TspGWI ACGGA 3 cut(s) 75, 271, 355
TspRI CASTG 3 cut(s) 418, 572, 682
VpaK11BI GGWCC 1 cut(s) 861
VspI ATTAAT 1 cut(s) 542
XapI RAATTY 2 cut(s) 723, 752
XceI RCATGY 1 cut(s) 801
XspI CTAG 2 cut(s) 404, 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.