AT5G66650

Mitochondrial calcium uniporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011209
Physical Location & Seq
Reverse (-)
26603003 .. 26607012
4010 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G66650.1

Sequence Viewer

Length: 966 bp
ATGTCGTCGAAGAAATCCCTAGTTCAAAGTCTCTTCAACATTTCCAAAACCTACTCCAGGATTTCTGGTCTTACCCGAATGCGTCCGACCAAATCCGGCGGCATTCCTCCCGACGCCGGAGATTCTGGAATCCGCCGCAGATTTCTCCACAAGAGGGCATTTTTCTCGCCGGAGATAGTTCCTAAAGGTGGTAACTTGATGGAGAAACTCAGGGAATTGACTTTGTCCAATAATAATCGTATTAGACTCGACGAGATGTTACCGCCACCTTCGCCGAAGAAATCATCACCGGAGTTTTTCCCGGCGGTCACGGTGGAAGACGTGAAGAAGCTCATGAGAGCAGCGGAAATGGAGCTGGTGAAATCGAAGCTGAGAGAGATTGGCAAGAACTGGGTTCCTTATTCGGAGTTTGTTCGGGTCTGCGGAGAATACAGTTCGGATCCTGAACAAGGTAACCGGGTCGCGAATATGCTTGACGAAGCTGGAAACGTCATCGTTTTGGGAAAACTCGTCTGCCTTAAACCCGAAGAGCTAACAAGCGCCATGGCTGGTCTGATTCCGACACTCGAACCCAGTCTCGACGCCGAGACAAGACAAGAGTTCGAACAACTTGAGATCATAAAATCAGATATCGACAAAAGAGCCGATGATCTGGTTCGAAAAGAATTATGGGCCGGATTAGGCCTAATAATGGCCCAAACAGTTGGATTTTTTAGGCTGACGTTTTGGGAACTGTCGTGGGACGTGATGGAACCCATATGCTTCTACGTAACTTCGACATATTTCATGGCTGGTTACGCCTTCTTCCTCCGAACTTCAAAGGAACCTTCCTTTGAAGGTTTTTACAAAAGCCGGTTCGAGACGAAGCAGAAACGTTTGATTAAAATGCTTGATTTCGATATCGATCGATTTACCAAGCTACAGAAGATGCATCGTCCAAATTTGACTAAATCTGGTCGTTGTTGA

Protein Analysis

321

Amino Acids

36.77

Weight (kDa)

9.35

Isoelectric Point (pI)

52.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MCU PF04678 149 - 307 1e-48 Mitochondrial calcium uniporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011209)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23790 AT4G36820 AT5G66650
fragaria_vesca FvH4_1g14090
malus_domestica MD02G1155000.v1.1 MD15G1269400.v1.1 MD15G1269500.v1.1
prunus_persica Prupe.7G148300_v2.0.a1
pyrus_communis pycom02g12270 pycom15g23380
rosa_chinensis RchiOBHm_Chr2g0102751
rosa_laevigata RLG00000017177
rosa_multiflora Rmu_co8164376.1_g000001
rosa_roxburghii Rroxscaffold_2G00140340
rosa_rugosa Rorug02G0109400
rosa_samantha Rh2AG157700 Rh2BG164200 Rh2CG163900 Rh2DG163000
rosa_wichuraiana Rw2G012230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 464
AciI CCGC 7 cut(s) 99, 133, 136, 263, 305, 344, 423
AclI AACGTT 1 cut(s) 874
AclWI GGATC 2 cut(s) 434, 447
AcsI RAATTY 1 cut(s) 940
AcyI GRCGYC 2 cut(s) 114, 582
AfiI CCNNNNNNNGG 6 cut(s) 57, 116, 154, 188, 449, 691
AgsI TTSAA 4 cut(s) 26, 37, 819, 836
AjiI CACGTC 2 cut(s) 322, 745
AjnI CCWGG 1 cut(s) 56
AluBI AGCT 6 cut(s) 331, 355, 370, 482, 532, 919
AluI AGCT 6 cut(s) 331, 355, 370, 482, 532, 919
Alw26I GTCTC 4 cut(s) 35, 581, 581, 854
AlwI GGATC 2 cut(s) 434, 447
AoxI GGCC 3 cut(s) 672, 682, 693
ApeKI GCWGC 1 cut(s) 341
ApoI RAATTY 1 cut(s) 940
AspLEI GCGC 1 cut(s) 542
AspS9I GGNCC 2 cut(s) 672, 694
AsuC2I CCSGG 2 cut(s) 302, 458
AsuHPI GGTGA 2 cut(s) 279, 370
AsuII TTCGAA 2 cut(s) 603, 658
BamHI GGATCC 1 cut(s) 439
BbsI GAAGAC 1 cut(s) 324
BbvI GCAGC 1 cut(s) 353
BccI CCATC 2 cut(s) 193, 742
BcgI CGANNNNNNTGC 2 cut(s) 147, 181
BciT130I CCWGG 1 cut(s) 58
BcnI CCSGG 2 cut(s) 302, 458
BcoDI GTCTC 4 cut(s) 35, 581, 581, 854
BfaI CTAG 1 cut(s) 20
BfmI CTRYAG 1 cut(s) 920
BfoI RGCGCY 1 cut(s) 543
BisI GCNGC 3 cut(s) 100, 136, 342
BlsI GCNGC 3 cut(s) 101, 137, 343
Bme1390I CCNGG 3 cut(s) 58, 302, 458
BmgBI CACGTC 2 cut(s) 322, 745
BmgT120I GGNCC 2 cut(s) 672, 694
BmiI GGNNCC 4 cut(s) 396, 441, 753, 825
BmrFI CCNGG 3 cut(s) 58, 302, 458
BmrI ACTGGG 2 cut(s) 400, 567
BmsI GCATC 2 cut(s) 918, 940
BmuI ACTGGG 2 cut(s) 400, 567
BpiI GAAGAC 1 cut(s) 324
BpmI CTGGAG 1 cut(s) 40
Bpu14I TTCGAA 2 cut(s) 603, 658
BpuEI CTTGAG 1 cut(s) 632
BpuMI CCSGG 2 cut(s) 302, 458
Bsa29I ATCGAT 2 cut(s) 903, 907
BsaAI YACGTR 1 cut(s) 769
BsaBI GATNNNNATC 1 cut(s) 903
BsaHI GRCGYC 2 cut(s) 114, 582
BsaJI CCNNGG 1 cut(s) 543
BsaWI WCCGGW 1 cut(s) 289
Bsc4I CCNNNNNNNGG 6 cut(s) 57, 116, 154, 188, 449, 691
Bse118I RCCGGY 1 cut(s) 852
Bse1I ACTGG 2 cut(s) 395, 573
Bse8I GATNNNNATC 1 cut(s) 903
BseBI CCWGG 1 cut(s) 58
BseCI ATCGAT 2 cut(s) 903, 907
BseDI CCNNGG 1 cut(s) 543
BseJI GATNNNNATC 1 cut(s) 903
BseLI CCNNNNNNNGG 6 cut(s) 57, 116, 154, 188, 449, 691
BseMII CTCAG 2 cut(s) 223, 362
BseNI ACTGG 2 cut(s) 395, 573
BseXI GCAGC 1 cut(s) 353
Bsh1236I CGCG 1 cut(s) 464
Bsh1285I CGRYCG 1 cut(s) 907
BshFI GGCC 3 cut(s) 674, 684, 695
BshVI ATCGAT 2 cut(s) 903, 907
BsiEI CGRYCG 1 cut(s) 907
BsiSI CCGG 8 cut(s) 96, 117, 170, 290, 302, 457, 675, 853
BslFI GGGAC 1 cut(s) 755
BslI CCNNNNNNNGG 6 cut(s) 57, 116, 154, 188, 449, 691
BsmAI GTCTC 4 cut(s) 35, 581, 581, 854
BsmBI CGTCTC 1 cut(s) 854
BsmFI GGGAC 1 cut(s) 755
BsmI GAATGC 2 cut(s) 84, 102
BsnI GGCC 3 cut(s) 674, 684, 695
Bsp119I TTCGAA 2 cut(s) 603, 658
Bsp143I GATC 4 cut(s) 439, 615, 649, 904
Bsp19I CCATGG 1 cut(s) 543
Bsp68I TCGCGA 1 cut(s) 464
BspACI CCGC 7 cut(s) 99, 133, 136, 263, 305, 344, 423
BspANI GGCC 3 cut(s) 674, 684, 695
BspCNI CTCAG 2 cut(s) 222, 363
BspDI ATCGAT 2 cut(s) 903, 907
BspFNI CGCG 1 cut(s) 464
BspHI TCATGA 1 cut(s) 333
BspLI GGNNCC 4 cut(s) 396, 441, 753, 825
BspPI GGATC 2 cut(s) 434, 447
BspQI GCTCTTC 1 cut(s) 522
BspT104I TTCGAA 2 cut(s) 603, 658
BsrFI RCCGGY 1 cut(s) 852
BsrI ACTGG 2 cut(s) 395, 573
BssAI RCCGGY 1 cut(s) 852
BssECI CCNNGG 1 cut(s) 543
BssMI GATC 4 cut(s) 439, 615, 649, 904
BssNI GRCGYC 2 cut(s) 114, 582
BssT1I CCWWGG 1 cut(s) 543
Bst2UI CCWGG 1 cut(s) 58
Bst4CI ACNGT 4 cut(s) 313, 434, 703, 735
Bst6I CTCTTC 2 cut(s) 38, 522
BstACI GRCGYC 2 cut(s) 114, 582
BstBAI YACGTR 1 cut(s) 769
BstBI TTCGAA 2 cut(s) 603, 658
BstDEI CTNAG 2 cut(s) 209, 371
BstDSI CCRYGG 1 cut(s) 543
BstEII GGTNACC 1 cut(s) 452
BstENI CCTNNNNNAGG 2 cut(s) 55, 447
BstFNI CGCG 1 cut(s) 464
BstH2I RGCGCY 1 cut(s) 543
BstHHI GCGC 1 cut(s) 542
BstKTI GATC 4 cut(s) 442, 618, 652, 907
BstMAI GTCTC 4 cut(s) 35, 581, 581, 854
BstMBI GATC 4 cut(s) 439, 615, 649, 904
BstMCI CGRYCG 1 cut(s) 907
BstMWI GCNNNNNNNGC 2 cut(s) 271, 797
BstNI CCWGG 1 cut(s) 58
BstPI GGTNACC 1 cut(s) 452
BstSCI CCNGG 3 cut(s) 56, 300, 456
BstSFI CTRYAG 1 cut(s) 920
BstSNI TACGTA 1 cut(s) 769
BstUI CGCG 1 cut(s) 464
BstV1I GCAGC 1 cut(s) 353
BstV2I GAAGAC 1 cut(s) 324
BstX2I RGATCY 1 cut(s) 439
BstXI CCANNNNNNTGG 1 cut(s) 704
BstYI RGATCY 1 cut(s) 439
Bsu15I ATCGAT 2 cut(s) 903, 907
BsuRI GGCC 3 cut(s) 674, 684, 695
BsuTUI ATCGAT 2 cut(s) 903, 907
BtgI CCRYGG 1 cut(s) 543
BtrI CACGTC 2 cut(s) 322, 745
BtuMI TCGCGA 1 cut(s) 464
CciI TCATGA 1 cut(s) 333
CfoI GCGC 1 cut(s) 542
Cfr10I RCCGGY 1 cut(s) 852
Cfr13I GGNCC 2 cut(s) 672, 694
ClaI ATCGAT 2 cut(s) 903, 907
CseI GACGC 3 cut(s) 71, 122, 590
CviAII CATG 3 cut(s) 334, 544, 787
DdeI CTNAG 2 cut(s) 209, 371
DpnI GATC 4 cut(s) 441, 617, 651, 906
DpnII GATC 4 cut(s) 439, 615, 649, 904
Eam1104I CTCTTC 2 cut(s) 38, 522
EarI CTCTTC 2 cut(s) 38, 522
EciI GGCGGA 1 cut(s) 122
Eco105I TACGTA 1 cut(s) 769
Eco130I CCWWGG 1 cut(s) 543
Eco147I AGGCCT 1 cut(s) 684
Eco32I GATATC 2 cut(s) 631, 901
Eco91I GGTNACC 1 cut(s) 452
EcoNI CCTNNNNNAGG 2 cut(s) 55, 447
EcoO65I GGTNACC 1 cut(s) 452
EcoRII CCWGG 1 cut(s) 56
EcoRV GATATC 2 cut(s) 631, 901
EcoT14I CCWWGG 1 cut(s) 543
EcoT22I ATGCAT 1 cut(s) 933
ErhI CCWWGG 1 cut(s) 543
Esp3I CGTCTC 1 cut(s) 854
FaeI CATG 3 cut(s) 337, 547, 790
FaiI YATR 9 cut(s) 335, 470, 545, 620, 670, 758, 760, 781, 788
FaqI GGGAC 1 cut(s) 755
FatI CATG 3 cut(s) 333, 543, 786
FauNDI CATATG 1 cut(s) 758
Fnu4HI GCNGC 3 cut(s) 100, 136, 342
Fsp4HI GCNGC 3 cut(s) 100, 136, 342
FspBI CTAG 1 cut(s) 20
GlaI GCGC 1 cut(s) 541
GluI GCNGC 3 cut(s) 100, 136, 342
GsuI CTGGAG 1 cut(s) 40
HaeII RGCGCY 1 cut(s) 543
HaeIII GGCC 3 cut(s) 674, 684, 695
HapII CCGG 8 cut(s) 96, 117, 170, 290, 302, 457, 675, 853
HgaI GACGC 3 cut(s) 71, 122, 590
HhaI GCGC 1 cut(s) 542
Hin1I GRCGYC 2 cut(s) 114, 582
Hin1II CATG 3 cut(s) 337, 547, 790
Hin6I GCGC 1 cut(s) 540
HinP1I GCGC 1 cut(s) 540
HinfI GANTC 4 cut(s) 122, 129, 246, 556
HpaII CCGG 8 cut(s) 96, 117, 170, 290, 302, 457, 675, 853
HphI GGTGA 2 cut(s) 279, 370
Hpy188I TCNGA 7 cut(s) 87, 406, 439, 555, 561, 628, 812
Hpy188III TCNNGA 7 cut(s) 110, 126, 334, 443, 463, 578, 859
Hpy99I CGWCG 4 cut(s) 10, 116, 254, 584
HpyAV CCTTC 4 cut(s) 279, 811, 830, 837
HpyCH4III ACNGT 4 cut(s) 313, 434, 703, 735
HpyCH4IV ACGT 6 cut(s) 321, 489, 722, 744, 768, 874
HpyCH4V TGCA 1 cut(s) 931
HpyF10VI GCNNNNNNNGC 2 cut(s) 271, 797
HpyF3I CTNAG 2 cut(s) 209, 371
HpySE526I ACGT 6 cut(s) 321, 489, 722, 744, 768, 874
Hsp92I GRCGYC 2 cut(s) 114, 582
Hsp92II CATG 3 cut(s) 337, 547, 790
HspAI GCGC 1 cut(s) 540
Kzo9I GATC 4 cut(s) 439, 615, 649, 904
LguI GCTCTTC 1 cut(s) 522
LmnI GCTCC 1 cut(s) 352
Lsp1109I GCAGC 1 cut(s) 353
LweI GCATC 2 cut(s) 918, 940
MaeI CTAG 1 cut(s) 20
MaeII ACGT 6 cut(s) 321, 489, 722, 744, 768, 874
MaeIII GTNAC 6 cut(s) 191, 258, 307, 452, 769, 794
MalI GATC 4 cut(s) 441, 617, 651, 906
MboI GATC 4 cut(s) 439, 615, 649, 904
MboII GAAGA 8 cut(s) 22, 25, 289, 329, 337, 539, 796, 937
MflI RGATCY 1 cut(s) 439
MluCI AATT 3 cut(s) 215, 665, 940
MlyI GAGTC 1 cut(s) 240
MmeI TCCRAC 3 cut(s) 110, 584, 685
MnlI CCTC 3 cut(s) 117, 147, 818
Mph1103I ATGCAT 1 cut(s) 933
MseI TTAA 2 cut(s) 519, 882
MspA1I CMGCKG 1 cut(s) 344
MspI CCGG 8 cut(s) 96, 117, 170, 290, 302, 457, 675, 853
MspR9I CCNGG 3 cut(s) 58, 302, 458
Mva1269I GAATGC 2 cut(s) 84, 102
MvaI CCWGG 1 cut(s) 58
MvnI CGCG 1 cut(s) 464
MwoI GCNNNNNNNGC 2 cut(s) 271, 797
NciI CCSGG 2 cut(s) 302, 458
NcoI CCATGG 1 cut(s) 543
NdeI CATATG 1 cut(s) 758
NdeII GATC 4 cut(s) 439, 615, 649, 904
NlaIII CATG 3 cut(s) 337, 547, 790
NlaIV GGNNCC 4 cut(s) 396, 441, 753, 825
NmeAIII GCCGAG 1 cut(s) 610
NmuCI GTSAC 1 cut(s) 307
NruI TCGCGA 1 cut(s) 464
NsiI ATGCAT 1 cut(s) 933
NspV TTCGAA 2 cut(s) 603, 658
PagI TCATGA 1 cut(s) 333
PceI AGGCCT 1 cut(s) 684
PciSI GCTCTTC 1 cut(s) 522
PctI GAATGC 2 cut(s) 84, 102
PfeI GAWTC 3 cut(s) 122, 129, 556
PflFI GACNNNGTC 1 cut(s) 223
PfoI TCCNGGA 1 cut(s) 56
PkrI GCNGC 3 cut(s) 101, 137, 343
Ple19I CGATCG 1 cut(s) 907
PleI GAGTC 1 cut(s) 240
PpsI GAGTC 1 cut(s) 240
Ppu21I YACGTR 1 cut(s) 769
Psp1406I AACGTT 1 cut(s) 874
Psp6I CCWGG 1 cut(s) 56
PspEI GGTNACC 1 cut(s) 452
PspGI CCWGG 1 cut(s) 56
PspN4I GGNNCC 4 cut(s) 396, 441, 753, 825
PspPI GGNCC 2 cut(s) 672, 694
PsuI RGATCY 1 cut(s) 439
PsyI GACNNNGTC 1 cut(s) 223
PvuI CGATCG 1 cut(s) 907
RruI TCGCGA 1 cut(s) 464
SapI GCTCTTC 1 cut(s) 522
SaqAI TTAA 2 cut(s) 519, 882
SatI GCNGC 3 cut(s) 100, 136, 342
Sau3AI GATC 4 cut(s) 439, 615, 649, 904
Sau96I GGNCC 2 cut(s) 672, 694
SchI GAGTC 1 cut(s) 240
ScrFI CCNGG 3 cut(s) 58, 302, 458
SfaNI GCATC 2 cut(s) 918, 940
SfcI CTRYAG 1 cut(s) 920
SfuI TTCGAA 2 cut(s) 603, 658
SmlI CTYRAG 1 cut(s) 611
SmoI CTYRAG 1 cut(s) 611
SnaBI TACGTA 1 cut(s) 769
Sse9I AATT 3 cut(s) 215, 665, 940
SseBI AGGCCT 1 cut(s) 684
SsiI CCGC 7 cut(s) 99, 133, 136, 263, 305, 344, 423
SspMI CTAG 1 cut(s) 20
StuI AGGCCT 1 cut(s) 684
StyD4I CCNGG 3 cut(s) 56, 300, 456
StyI CCWWGG 1 cut(s) 543
TaaI ACNGT 4 cut(s) 313, 434, 703, 735
TaiI ACGT 6 cut(s) 324, 492, 725, 747, 771, 877
TasI AATT 3 cut(s) 215, 665, 940
TauI GCSGC 2 cut(s) 102, 138
TfiI GAWTC 3 cut(s) 122, 129, 556
Tru1I TTAA 2 cut(s) 519, 882
Tru9I TTAA 2 cut(s) 519, 882
TseFI GTSAC 1 cut(s) 307
TseI GCWGC 1 cut(s) 341
Tsp45I GTSAC 1 cut(s) 307
TspDTI ATGAA 1 cut(s) 775
Tth111I GACNNNGTC 1 cut(s) 223
XagI CCTNNNNNAGG 2 cut(s) 55, 447
XapI RAATTY 1 cut(s) 940
XspI CTAG 1 cut(s) 20
Zsp2I ATGCAT 1 cut(s) 933
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.