MD15G1269400.v1.1

Mitochondrial calcium uniporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0011209
Physical Location & Seq
Forward (+)
23289537 .. 23290692
1156 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1269400.v1.1.491

Sequence Viewer

Length: 588 bp
ATGGCGTTCAAGAAAACCCTAGCCGAGCGCCTGTTCAACATCTCCAAGGCGTCGACCCAAACCCTCAGAAACTGCCGGATTTCGTCGTCGGCGGTCCACACCCGACCCGCTCCCAAGCCCCGCGAGACCAGAATCGCCCCCGACCCTGGAGACAATGCCATTTTCCGGAGGTTCCTCCACAAGAGATCGTCGGCGGGGAATTTCCAGAAGCAGGAGATCTGGTCTTTGCCGATGGGGGCAAACCTGATGGAGAAGCTCAAAGTCGTGGCCATTGGCCGGGACCGGATCCGATTGGACGGTTTGGCCCCGCCGGAACCCGAGTGGAAGAGCTCGCCGATGGAGAAATCGGGATTGACGACGGAGGAAACGAAGAAACTGCTGAGGGTGGCCCAGCTGGAAGCCGTGAAATCGAAGCTTCGAGAAGTCCAGAAAAGCTGGGTGTCGTATCCGGAGTTTGTTGGGATCTGCCGGGAAGGTTGCGCGGATCCGGATATGGGTATCGGGTTTGCGAAATCGTTGGACGAAAACGGTTCCGTGATCGTATTGGGAAATTCCGTTTGCTTGAGGCCTGAACAGGCAGAGACCTAA

Protein Analysis

196

Amino Acids

21.72

Weight (kDa)

9.59

Isoelectric Point (pI)

39.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011209)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23790 AT4G36820 AT5G66650
fragaria_vesca FvH4_1g14090
malus_domestica MD02G1155000.v1.1 MD15G1269400.v1.1 MD15G1269500.v1.1
prunus_persica Prupe.7G148300_v2.0.a1
pyrus_communis pycom02g12270 pycom15g23380
rosa_chinensis RchiOBHm_Chr2g0102751
rosa_laevigata RLG00000017177
rosa_multiflora Rmu_co8164376.1_g000001
rosa_roxburghii Rroxscaffold_2G00140340
rosa_rugosa Rorug02G0109400
rosa_samantha Rh2AG157700 Rh2BG164200 Rh2CG163900 Rh2DG163000
rosa_wichuraiana Rw2G012230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 110
AccI GTMKAC 1 cut(s) 53
AccII CGCG 2 cut(s) 123, 482
AccIII TCCGGA 3 cut(s) 165, 448, 487
AciI CCGC 6 cut(s) 92, 108, 121, 194, 308, 482
AclWI GGATC 5 cut(s) 280, 293, 470, 479, 492
AcoI YGGCCR 2 cut(s) 267, 274
AcsI RAATTY 2 cut(s) 199, 550
AcyI GRCGYC 1 cut(s) 50
AfiI CCNNNNNNNGG 5 cut(s) 146, 165, 211, 276, 494
AgsI TTSAA 2 cut(s) 10, 37
AjnI CCWGG 1 cut(s) 145
AluBI AGCT 5 cut(s) 256, 330, 394, 415, 435
AluI AGCT 5 cut(s) 256, 330, 394, 415, 435
Alw21I GWGCWC 1 cut(s) 332
Alw26I GTCTC 3 cut(s) 119, 144, 575
AlwI GGATC 5 cut(s) 280, 293, 470, 479, 492
AlwNI CAGNNNCTG 1 cut(s) 72
Ama87I CYCGRG 1 cut(s) 317
Aor13HI TCCGGA 3 cut(s) 165, 448, 487
AoxI GGCC 5 cut(s) 267, 274, 303, 387, 566
ApoI RAATTY 2 cut(s) 199, 550
AspLEI GCGC 2 cut(s) 30, 482
AspS9I GGNCC 4 cut(s) 94, 280, 304, 388
AsuC2I CCSGG 2 cut(s) 278, 470
AvaI CYCGRG 1 cut(s) 317
AvaII GGWCC 2 cut(s) 94, 280
BalI TGGCCA 1 cut(s) 269
BamHI GGATCC 2 cut(s) 285, 484
BanII GRGCYC 1 cut(s) 332
Bbv12I GWGCWC 1 cut(s) 332
BbvCI CCTCAGC 1 cut(s) 380
BccI CCATC 3 cut(s) 226, 241, 331
BceAI ACGGC 1 cut(s) 386
BcgI CGANNNNNNTGC 2 cut(s) 358, 392
BciT130I CCWGG 1 cut(s) 147
BciVI GTATCC 1 cut(s) 456
BcnI CCSGG 2 cut(s) 278, 470
BcoDI GTCTC 3 cut(s) 119, 144, 575
BfaI CTAG 1 cut(s) 20
BfoI RGCGCY 1 cut(s) 31
BfuI GTATCC 1 cut(s) 456
BglII AGATCT 1 cut(s) 216
Bme1390I CCNGG 3 cut(s) 147, 278, 470
Bme18I GGWCC 2 cut(s) 94, 280
BmeT110I CYCGRG 1 cut(s) 317
BmgT120I GGNCC 4 cut(s) 94, 280, 304, 388
BmiI GGNNCC 7 cut(s) 173, 281, 287, 306, 315, 486, 532
BmrFI CCNGG 3 cut(s) 147, 278, 470
BpmI CTGGAG 1 cut(s) 168
Bpu10I CCTNAGC 1 cut(s) 380
BpuEI CTTGAG 1 cut(s) 583
BpuMI CCSGG 2 cut(s) 278, 470
BsaHI GRCGYC 1 cut(s) 50
BsaI GGTCTC 2 cut(s) 119, 575
BsaJI CCNNGG 2 cut(s) 45, 145
BsaWI WCCGGW 4 cut(s) 165, 282, 448, 487
BsaXI ACNNNNNCTCC 2 cut(s) 206, 236
Bsc4I CCNNNNNNNGG 5 cut(s) 146, 165, 211, 276, 494
BseAI TCCGGA 3 cut(s) 165, 448, 487
BseBI CCWGG 1 cut(s) 147
BseDI CCNNGG 2 cut(s) 45, 145
BseLI CCNNNNNNNGG 5 cut(s) 146, 165, 211, 276, 494
BseMII CTCAG 2 cut(s) 79, 371
BseYI CCCAGC 2 cut(s) 390, 435
Bsh1236I CGCG 2 cut(s) 123, 482
BshFI GGCC 5 cut(s) 269, 276, 305, 389, 568
BsiHKAI GWGCWC 1 cut(s) 332
BsiHKCI CYCGRG 1 cut(s) 317
BsiSI CCGG 8 cut(s) 76, 166, 277, 283, 311, 449, 469, 488
BslFI GGGAC 1 cut(s) 293
BslI CCNNNNNNNGG 5 cut(s) 146, 165, 211, 276, 494
BsmAI GTCTC 3 cut(s) 119, 144, 575
BsmFI GGGAC 1 cut(s) 293
BsnI GGCC 5 cut(s) 269, 276, 305, 389, 568
Bso31I GGTCTC 2 cut(s) 119, 575
BsoBI CYCGRG 1 cut(s) 317
Bsp1286I GDGCHC 1 cut(s) 332
Bsp13I TCCGGA 3 cut(s) 165, 448, 487
Bsp143I GATC 6 cut(s) 185, 216, 285, 462, 484, 537
BspACI CCGC 6 cut(s) 92, 108, 121, 194, 308, 482
BspANI GGCC 5 cut(s) 269, 276, 305, 389, 568
BspCNI CTCAG 2 cut(s) 78, 372
BspEI TCCGGA 3 cut(s) 165, 448, 487
BspFNI CGCG 2 cut(s) 123, 482
BspLI GGNNCC 7 cut(s) 173, 281, 287, 306, 315, 486, 532
BspPI GGATC 5 cut(s) 280, 293, 470, 479, 492
BspQI GCTCTTC 1 cut(s) 320
BspTNI GGTCTC 2 cut(s) 119, 575
BsrBI CCGCTC 1 cut(s) 110
BssECI CCNNGG 2 cut(s) 45, 145
BssMI GATC 6 cut(s) 185, 216, 285, 462, 484, 537
BssNI GRCGYC 1 cut(s) 50
BssT1I CCWWGG 1 cut(s) 45
Bst2UI CCWGG 1 cut(s) 147
Bst4CI ACNGT 2 cut(s) 299, 530
Bst6I CTCTTC 1 cut(s) 320
BstACI GRCGYC 1 cut(s) 50
BstC8I GCNNGC 1 cut(s) 332
BstDEI CTNAG 2 cut(s) 65, 380
BstFNI CGCG 2 cut(s) 123, 482
BstH2I RGCGCY 1 cut(s) 31
BstHHI GCGC 2 cut(s) 30, 482
BstKTI GATC 6 cut(s) 188, 219, 288, 465, 487, 540
BstMAI GTCTC 3 cut(s) 119, 144, 575
BstMBI GATC 6 cut(s) 185, 216, 285, 462, 484, 537
BstNI CCWGG 1 cut(s) 147
BstSCI CCNGG 3 cut(s) 145, 276, 468
BstUI CGCG 2 cut(s) 123, 482
BstX2I RGATCY 4 cut(s) 216, 285, 462, 484
BstYI RGATCY 4 cut(s) 216, 285, 462, 484
BsuI GTATCC 1 cut(s) 456
BsuRI GGCC 5 cut(s) 269, 276, 305, 389, 568
Cac8I GCNNGC 1 cut(s) 332
CaiI CAGNNNCTG 1 cut(s) 72
CfoI GCGC 2 cut(s) 30, 482
Cfr13I GGNCC 4 cut(s) 94, 280, 304, 388
CseI GACGC 1 cut(s) 39
DdeI CTNAG 2 cut(s) 65, 380
DpnI GATC 6 cut(s) 187, 218, 287, 464, 486, 539
DpnII GATC 6 cut(s) 185, 216, 285, 462, 484, 537
EaeI YGGCCR 2 cut(s) 267, 274
Eam1104I CTCTTC 1 cut(s) 320
EarI CTCTTC 1 cut(s) 320
Ecl136II GAGCTC 1 cut(s) 330
Eco130I CCWWGG 1 cut(s) 45
Eco147I AGGCCT 1 cut(s) 568
Eco24I GRGCYC 1 cut(s) 332
Eco31I GGTCTC 2 cut(s) 119, 575
Eco47I GGWCC 2 cut(s) 94, 280
Eco53kI GAGCTC 1 cut(s) 330
Eco88I CYCGRG 1 cut(s) 317
EcoICRI GAGCTC 1 cut(s) 330
EcoRII CCWGG 1 cut(s) 145
EcoT14I CCWWGG 1 cut(s) 45
EcoT38I GRGCYC 1 cut(s) 332
ErhI CCWWGG 1 cut(s) 45
FaiI YATR 1 cut(s) 494
FaqI GGGAC 1 cut(s) 293
FauI CCCGC 4 cut(s) 115, 128, 187, 315
FblI GTMKAC 1 cut(s) 53
FriOI GRGCYC 1 cut(s) 332
FspBI CTAG 1 cut(s) 20
GlaI GCGC 2 cut(s) 29, 481
GsaI CCCAGC 2 cut(s) 394, 439
GsuI CTGGAG 1 cut(s) 168
HaeII RGCGCY 1 cut(s) 31
HaeIII GGCC 5 cut(s) 269, 276, 305, 389, 568
HapII CCGG 8 cut(s) 76, 166, 277, 283, 311, 449, 469, 488
HgaI GACGC 1 cut(s) 39
HhaI GCGC 2 cut(s) 30, 482
Hin1I GRCGYC 1 cut(s) 50
Hin6I GCGC 2 cut(s) 28, 480
HinP1I GCGC 2 cut(s) 28, 480
HincII GTYRAC 1 cut(s) 54
HindII GTYRAC 1 cut(s) 54
HindIII AAGCTT 1 cut(s) 413
HinfI GANTC 1 cut(s) 132
HpaII CCGG 8 cut(s) 76, 166, 277, 283, 311, 449, 469, 488
Hpy166II GTNNAC 2 cut(s) 54, 97
Hpy188I TCNGA 2 cut(s) 68, 290
Hpy188III TCNNGA 8 cut(s) 10, 166, 205, 348, 419, 427, 449, 488
Hpy8I GTNNAC 2 cut(s) 54, 97
Hpy99I CGWCG 5 cut(s) 55, 88, 91, 193, 361
HpyAV CCTTC 1 cut(s) 467
HpyCH4III ACNGT 2 cut(s) 299, 530
HpyF3I CTNAG 2 cut(s) 65, 380
Hsp92I GRCGYC 1 cut(s) 50
HspAI GCGC 2 cut(s) 28, 480
Kpn2I TCCGGA 3 cut(s) 165, 448, 487
Kzo9I GATC 6 cut(s) 185, 216, 285, 462, 484, 537
LguI GCTCTTC 1 cut(s) 320
LmnI GCTCC 1 cut(s) 115
MaeI CTAG 1 cut(s) 20
MalI GATC 6 cut(s) 187, 218, 287, 464, 486, 539
MbiI CCGCTC 1 cut(s) 110
MboI GATC 6 cut(s) 185, 216, 285, 462, 484, 537
MboII GAAGA 2 cut(s) 337, 382
MflI RGATCY 4 cut(s) 216, 285, 462, 484
MhlI GDGCHC 1 cut(s) 332
MlsI TGGCCA 1 cut(s) 269
MluCI AATT 2 cut(s) 199, 550
MluNI TGGCCA 1 cut(s) 269
MmeI TCCRAC 1 cut(s) 498
MnlI CCTC 6 cut(s) 74, 162, 185, 355, 375, 558
Mox20I TGGCCA 1 cut(s) 269
MroI TCCGGA 3 cut(s) 165, 448, 487
MscI TGGCCA 1 cut(s) 269
Msp20I TGGCCA 1 cut(s) 269
MspA1I CMGCKG 1 cut(s) 394
MspI CCGG 8 cut(s) 76, 166, 277, 283, 311, 449, 469, 488
MspR9I CCNGG 3 cut(s) 147, 278, 470
MvaI CCWGG 1 cut(s) 147
MvnI CGCG 2 cut(s) 123, 482
NciI CCSGG 2 cut(s) 278, 470
NdeII GATC 6 cut(s) 185, 216, 285, 462, 484, 537
NlaIV GGNNCC 7 cut(s) 173, 281, 287, 306, 315, 486, 532
NmeAIII GCCGAG 1 cut(s) 49
PceI AGGCCT 1 cut(s) 568
PciSI GCTCTTC 1 cut(s) 320
PcsI WCGNNNNNNNCGW 2 cut(s) 353, 365
PfeI GAWTC 1 cut(s) 132
Psp124BI GAGCTC 1 cut(s) 332
Psp6I CCWGG 1 cut(s) 145
PspFI CCCAGC 2 cut(s) 390, 435
PspGI CCWGG 1 cut(s) 145
PspN4I GGNNCC 7 cut(s) 173, 281, 287, 306, 315, 486, 532
PspPI GGNCC 4 cut(s) 94, 280, 304, 388
PstNI CAGNNNCTG 1 cut(s) 72
PsuI RGATCY 4 cut(s) 216, 285, 462, 484
PvuII CAGCTG 1 cut(s) 394
SacI GAGCTC 1 cut(s) 332
SalI GTCGAC 1 cut(s) 52
SapI GCTCTTC 1 cut(s) 320
Sau3AI GATC 6 cut(s) 185, 216, 285, 462, 484, 537
Sau96I GGNCC 4 cut(s) 94, 280, 304, 388
ScrFI CCNGG 3 cut(s) 147, 278, 470
SduI GDGCHC 1 cut(s) 332
SetI ASST 9 cut(s) 173, 246, 258, 332, 396, 417, 437, 478, 587
SinI GGWCC 2 cut(s) 94, 280
SmlI CTYRAG 1 cut(s) 562
SmoI CTYRAG 1 cut(s) 562
Sse9I AATT 2 cut(s) 199, 550
SseBI AGGCCT 1 cut(s) 568
SsiI CCGC 6 cut(s) 92, 108, 121, 194, 308, 482
SspMI CTAG 1 cut(s) 20
SstI GAGCTC 1 cut(s) 332
StuI AGGCCT 1 cut(s) 568
StyD4I CCNGG 3 cut(s) 145, 276, 468
StyI CCWWGG 1 cut(s) 45
TaaI ACNGT 2 cut(s) 299, 530
TaqI TCGA 3 cut(s) 53, 410, 418
TasI AATT 2 cut(s) 199, 550
TfiI GAWTC 1 cut(s) 132
TspGWI ACGGA 3 cut(s) 374, 523, 544
VpaK11BI GGWCC 2 cut(s) 94, 280
XapI RAATTY 2 cut(s) 199, 550
XmiI GTMKAC 1 cut(s) 53
XspI CTAG 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.