MD02G1155000.v1.1

Mitochondrial calcium uniporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0011209
Physical Location & Seq
Forward (+)
12850382 .. 12853073
2692 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1155000.v1.1.491

Sequence Viewer

Length: 1071 bp
ATGGCGTTCAAGAAAACCCTAGCCCAGCGTCTGTTCAACATCTCCAAGGCGTCGACCCAAACCCTCAGAAACTGCCGGATTTCGTCGTCGGCGGTCCACATCCGACCCGCTTCCAAGCCTCGCGAGACGAGCATCGCGCCCGACCCGGGTGACAATGCCATGTTCCGGAGGTTCCTCCACAAGACACCGTCGGCGGGAAATTTCCAGAAGCCAGCGATCTGGTCTTTGCCGATGGGGGAAAACCTAATGGAGAAGCTCAAAGTCATGGCCATTGGCCAGGATCGGATCCGATTGGACGGTTTGGCTCCGCCGGAGCCCGAGCCGGAGAGCTTGCCGGTGGCAAAATTGGGATTGACGACGGAGGAAACAAAGAAGCTGCTAAGGGTGGCCCAGCTGGAGGCGGTGAAATTGAAGCTTCGAGAAGTCCGGAAAAGCTGGGTATCGTATTCGGAGTTTGTTCGGATCTGCAGGGAAAGCTGCGAGGATCCGGATATGGGTCTGGGGTTTGCGAAATCGCTGGACGAAAACGGCTCCGTCATCGTATTGGGAAATTCCGTTTGTTTGAAGCCCGAACAGGTAGCCAAAGCAATTCAAGAACTATTTCCCATTCCAGGAACACCACCCGACTCCGATGACCCCAGAATTAGGGAATTGGAAGACATGGAGAGGCACAAGTTTGAGATAGACCGAAAGGCCAAGTCCCTTGTCCGAAAGGAGCTTTGGTGCGGTCTTGGCTTTTTGGTTGTCCAAACAGCCGGATTCATGAGGCTCACGTTCTGGGAGCTCTCATGGGACATCATGGAACCCATCTGCTTCTACGTTACTTCAATGTATTTCATGGCTGGATACGCCTTCTTCCTCAGGACGTCGATAGAGCCCTCTTTCGAAGGGTTTTACCATAGCCGGTTTCAGGCTAAGCAGAAGCGCCTCATGAAGCTTGAGAATTTCGACGTCGGAAGGTATGACGAGCTTCGTAAAGCATGTTATCCGTACTCGTCGGATTCATCGGAAATGACCCCGACAACTGCTTTTGATGGGGCGAAGAGGAAGCAATTTGGTTCAGTACATTAG

Protein Analysis

357

Amino Acids

40.59

Weight (kDa)

9.03

Isoelectric Point (pI)

44.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MCU PF04678 164 - 324 9.8e-51 Mitochondrial calcium uniporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011209)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23790 AT4G36820 AT5G66650
fragaria_vesca FvH4_1g14090
malus_domestica MD02G1155000.v1.1 MD15G1269400.v1.1 MD15G1269500.v1.1
prunus_persica Prupe.7G148300_v2.0.a1
pyrus_communis pycom02g12270 pycom15g23380
rosa_chinensis RchiOBHm_Chr2g0102751
rosa_laevigata RLG00000017177
rosa_multiflora Rmu_co8164376.1_g000001
rosa_roxburghii Rroxscaffold_2G00140340
rosa_rugosa Rorug02G0109400
rosa_samantha Rh2AG157700 Rh2BG164200 Rh2CG163900 Rh2DG163000
rosa_wichuraiana Rw2G012230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 869, 954
AccI GTMKAC 1 cut(s) 53
AccII CGCG 2 cut(s) 123, 137
AccIII TCCGGA 3 cut(s) 165, 426, 487
AciI CCGC 6 cut(s) 92, 108, 194, 308, 401, 726
AclWI GGATC 6 cut(s) 280, 288, 293, 470, 479, 492
AcoI YGGCCR 2 cut(s) 267, 274
AcsI RAATTY 3 cut(s) 199, 550, 943
AcyI GRCGYC 3 cut(s) 50, 866, 951
AfaI GTAC 2 cut(s) 992, 1065
AfiI CCNNNNNNNGG 8 cut(s) 146, 165, 194, 397, 494, 611, 645, 910
AgsI TTSAA 6 cut(s) 10, 37, 412, 565, 593, 828
AjnI CCWGG 2 cut(s) 276, 610
AjuI GAANNNNNNNTTGG 2 cut(s) 703, 735
Alw21I GWGCWC 1 cut(s) 786
Alw26I GTCTC 1 cut(s) 119
AlwI GGATC 6 cut(s) 280, 288, 293, 470, 479, 492
AlwNI CAGNNNCTG 2 cut(s) 31, 72
Ama87I CYCGRG 2 cut(s) 145, 317
Aor13HI TCCGGA 3 cut(s) 165, 426, 487
AoxI GGCC 4 cut(s) 267, 274, 387, 693
ApeKI GCWGC 2 cut(s) 376, 477
ApoI RAATTY 3 cut(s) 199, 550, 943
Asp700I GAANNNNTTC 1 cut(s) 600
AspLEI GCGC 2 cut(s) 139, 927
AspS9I GGNCC 2 cut(s) 94, 388
AsuC2I CCSGG 2 cut(s) 146, 147
AsuHPI GGTGA 2 cut(s) 161, 415
AsuII TTCGAA 1 cut(s) 885
AvaI CYCGRG 2 cut(s) 145, 317
AvaII GGWCC 1 cut(s) 94
AxyI CCTNAGG 1 cut(s) 860
BalI TGGCCA 2 cut(s) 269, 276
BamHI GGATCC 2 cut(s) 285, 484
BanII GRGCYC 3 cut(s) 318, 786, 879
BbsI GAAGAC 1 cut(s) 663
Bbv12I GWGCWC 1 cut(s) 786
BbvI GCAGC 2 cut(s) 363, 464
BccI CCATC 3 cut(s) 226, 815, 1028
BceAI ACGGC 1 cut(s) 544
BciT130I CCWGG 2 cut(s) 278, 612
BciVI GTATCC 1 cut(s) 839
BcnI CCSGG 2 cut(s) 146, 147
BcoDI GTCTC 1 cut(s) 119
BfaI CTAG 1 cut(s) 20
BfmI CTRYAG 1 cut(s) 466
BfoI RGCGCY 1 cut(s) 928
BfuI GTATCC 1 cut(s) 839
BisI GCNGC 2 cut(s) 377, 478
BlpI GCTNAGC 1 cut(s) 915
BlsI GCNGC 2 cut(s) 378, 479
Bme1390I CCNGG 4 cut(s) 146, 147, 278, 612
Bme18I GGWCC 1 cut(s) 94
BmeT110I CYCGRG 2 cut(s) 145, 317
BmgT120I GGNCC 2 cut(s) 94, 388
BmiI GGNNCC 7 cut(s) 173, 287, 306, 315, 486, 532, 804
BmrFI CCNGG 4 cut(s) 146, 147, 278, 612
BmsI GCATC 1 cut(s) 141
BpiI GAAGAC 1 cut(s) 663
BpmI CTGGAG 1 cut(s) 416
Bpu10I CCTNAGC 1 cut(s) 380
Bpu1102I GCTNAGC 1 cut(s) 915
Bpu14I TTCGAA 1 cut(s) 885
BpuEI CTTGAG 1 cut(s) 959
BpuMI CCSGG 2 cut(s) 146, 147
BsaHI GRCGYC 3 cut(s) 50, 866, 951
BsaJI CCNNGG 2 cut(s) 45, 145
BsaWI WCCGGW 3 cut(s) 165, 426, 487
BsaXI ACNNNNNCTCC 2 cut(s) 707, 737
Bsc4I CCNNNNNNNGG 8 cut(s) 146, 165, 194, 397, 494, 611, 645, 910
Bse118I RCCGGY 2 cut(s) 334, 903
Bse21I CCTNAGG 1 cut(s) 860
BseAI TCCGGA 3 cut(s) 165, 426, 487
BseBI CCWGG 2 cut(s) 278, 612
BseDI CCNNGG 2 cut(s) 45, 145
BseGI GGATG 1 cut(s) 99
BseLI CCNNNNNNNGG 8 cut(s) 146, 165, 194, 397, 494, 611, 645, 910
BseMII CTCAG 2 cut(s) 79, 874
BseXI GCAGC 2 cut(s) 363, 464
BseYI CCCAGC 3 cut(s) 24, 390, 435
Bsh1236I CGCG 2 cut(s) 123, 137
BshFI GGCC 4 cut(s) 269, 276, 389, 695
BsiHKAI GWGCWC 1 cut(s) 786
BsiHKCI CYCGRG 2 cut(s) 145, 317
BslFI GGGAC 2 cut(s) 685, 806
BslI CCNNNNNNNGG 8 cut(s) 146, 165, 194, 397, 494, 611, 645, 910
BsmAI GTCTC 1 cut(s) 119
BsmBI CGTCTC 1 cut(s) 119
BsmFI GGGAC 2 cut(s) 685, 806
BsnI GGCC 4 cut(s) 269, 276, 389, 695
BsoBI CYCGRG 2 cut(s) 145, 317
Bsp119I TTCGAA 1 cut(s) 885
Bsp1286I GDGCHC 3 cut(s) 318, 786, 879
Bsp13I TCCGGA 3 cut(s) 165, 426, 487
Bsp143I GATC 5 cut(s) 216, 280, 285, 462, 484
Bsp1720I GCTNAGC 1 cut(s) 915
Bsp68I TCGCGA 1 cut(s) 123
BspACI CCGC 6 cut(s) 92, 108, 194, 308, 401, 726
BspANI GGCC 4 cut(s) 269, 276, 389, 695
BspCNI CTCAG 2 cut(s) 78, 873
BspEI TCCGGA 3 cut(s) 165, 426, 487
BspFNI CGCG 2 cut(s) 123, 137
BspHI TCATGA 2 cut(s) 762, 930
BspLI GGNNCC 7 cut(s) 173, 287, 306, 315, 486, 532, 804
BspMAI CTGCAG 1 cut(s) 470
BspPI GGATC 6 cut(s) 280, 288, 293, 470, 479, 492
BspT104I TTCGAA 1 cut(s) 885
BsrFI RCCGGY 2 cut(s) 334, 903
BssAI RCCGGY 2 cut(s) 334, 903
BssECI CCNNGG 2 cut(s) 45, 145
BssMI GATC 5 cut(s) 216, 280, 285, 462, 484
BssNI GRCGYC 3 cut(s) 50, 866, 951
BssT1I CCWWGG 1 cut(s) 45
Bst2UI CCWGG 2 cut(s) 278, 612
Bst4CI ACNGT 2 cut(s) 189, 299
Bst6I CTCTTC 1 cut(s) 1037
BstACI GRCGYC 3 cut(s) 50, 866, 951
BstBI TTCGAA 1 cut(s) 885
BstC8I GCNNGC 2 cut(s) 213, 332
BstDEI CTNAG 4 cut(s) 65, 380, 860, 915
BstF5I GGATG 1 cut(s) 99
BstFNI CGCG 2 cut(s) 123, 137
BstH2I RGCGCY 1 cut(s) 928
BstHHI GCGC 2 cut(s) 139, 927
BstKTI GATC 5 cut(s) 219, 283, 288, 465, 487
BstMAI GTCTC 1 cut(s) 119
BstMBI GATC 5 cut(s) 216, 280, 285, 462, 484
BstMWI GCNNNNNNNGC 4 cut(s) 129, 474, 732, 848
BstNI CCWGG 2 cut(s) 278, 612
BstNSI RCATGY 1 cut(s) 984
BstSCI CCNGG 4 cut(s) 144, 145, 276, 610
BstSFI CTRYAG 1 cut(s) 466
BstUI CGCG 2 cut(s) 123, 137
BstV1I GCAGC 2 cut(s) 363, 464
BstV2I GAAGAC 1 cut(s) 663
BstX2I RGATCY 3 cut(s) 285, 462, 484
BstXI CCANNNNNNTGG 1 cut(s) 219
BstYI RGATCY 3 cut(s) 285, 462, 484
Bsu36I CCTNAGG 1 cut(s) 860
BsuI GTATCC 1 cut(s) 839
BsuRI GGCC 4 cut(s) 269, 276, 389, 695
BtgZI GCGATG 1 cut(s) 118
BtsCI GGATG 1 cut(s) 99
BtuMI TCGCGA 1 cut(s) 123
Cac8I GCNNGC 2 cut(s) 213, 332
CaiI CAGNNNCTG 2 cut(s) 31, 72
CciI TCATGA 2 cut(s) 762, 930
CfoI GCGC 2 cut(s) 139, 927
Cfr10I RCCGGY 2 cut(s) 334, 903
Cfr13I GGNCC 2 cut(s) 94, 388
Cfr9I CCCGGG 1 cut(s) 145
CseI GACGC 2 cut(s) 17, 39
Csp6I GTAC 2 cut(s) 991, 1064
CviAII CATG 9 cut(s) 160, 265, 661, 763, 789, 799, 838, 931, 981
CviQI GTAC 2 cut(s) 991, 1064
DdeI CTNAG 4 cut(s) 65, 380, 860, 915
DpnI GATC 5 cut(s) 218, 282, 287, 464, 486
DpnII GATC 5 cut(s) 216, 280, 285, 462, 484
EaeI YGGCCR 2 cut(s) 267, 274
Eam1104I CTCTTC 1 cut(s) 1037
EarI CTCTTC 1 cut(s) 1037
EciI GGCGGA 1 cut(s) 297
Ecl136II GAGCTC 1 cut(s) 784
Eco130I CCWWGG 1 cut(s) 45
Eco24I GRGCYC 3 cut(s) 318, 786, 879
Eco47I GGWCC 1 cut(s) 94
Eco53kI GAGCTC 1 cut(s) 784
Eco81I CCTNAGG 1 cut(s) 860
Eco88I CYCGRG 2 cut(s) 145, 317
EcoICRI GAGCTC 1 cut(s) 784
EcoRII CCWGG 2 cut(s) 276, 610
EcoT14I CCWWGG 1 cut(s) 45
EcoT38I GRGCYC 3 cut(s) 318, 786, 879
ErhI CCWWGG 1 cut(s) 45
Esp3I CGTCTC 1 cut(s) 119
FaeI CATG 9 cut(s) 163, 268, 664, 766, 792, 802, 841, 934, 984
FaqI GGGAC 2 cut(s) 685, 806
FatI CATG 9 cut(s) 159, 264, 660, 762, 788, 798, 837, 930, 980
FauI CCCGC 2 cut(s) 115, 187
FblI GTMKAC 1 cut(s) 53
Fnu4HI GCNGC 2 cut(s) 377, 478
FokI GGATG 1 cut(s) 86
FriOI GRGCYC 3 cut(s) 318, 786, 879
Fsp4HI GCNGC 2 cut(s) 377, 478
FspBI CTAG 1 cut(s) 20
GlaI GCGC 2 cut(s) 138, 926
GluI GCNGC 2 cut(s) 377, 478
GsaI CCCAGC 3 cut(s) 28, 394, 439
GsuI CTGGAG 1 cut(s) 416
HaeII RGCGCY 1 cut(s) 928
HaeIII GGCC 4 cut(s) 269, 276, 389, 695
HgaI GACGC 2 cut(s) 17, 39
HhaI GCGC 2 cut(s) 139, 927
Hin1I GRCGYC 3 cut(s) 50, 866, 951
Hin1II CATG 9 cut(s) 163, 268, 664, 766, 792, 802, 841, 934, 984
Hin6I GCGC 2 cut(s) 137, 925
HinP1I GCGC 2 cut(s) 137, 925
HincII GTYRAC 1 cut(s) 54
HindII GTYRAC 1 cut(s) 54
HindIII AAGCTT 2 cut(s) 413, 935
HinfI GANTC 3 cut(s) 626, 759, 1001
HphI GGTGA 2 cut(s) 161, 415
Hpy166II GTNNAC 2 cut(s) 54, 97
Hpy8I GTNNAC 2 cut(s) 54, 97
Hpy99I CGWCG 9 cut(s) 55, 88, 91, 193, 361, 871, 953, 956, 1000
HpyAV CCTTC 3 cut(s) 862, 881, 951
HpyCH4III ACNGT 2 cut(s) 189, 299
HpyCH4IV ACGT 4 cut(s) 773, 819, 866, 951
HpyCH4V TGCA 1 cut(s) 468
HpyF10VI GCNNNNNNNGC 4 cut(s) 129, 474, 732, 848
HpyF3I CTNAG 4 cut(s) 65, 380, 860, 915
HpySE526I ACGT 4 cut(s) 773, 819, 866, 951
Hsp92I GRCGYC 3 cut(s) 50, 866, 951
Hsp92II CATG 9 cut(s) 163, 268, 664, 766, 792, 802, 841, 934, 984
HspAI GCGC 2 cut(s) 137, 925
Kpn2I TCCGGA 3 cut(s) 165, 426, 487
Kzo9I GATC 5 cut(s) 216, 280, 285, 462, 484
LmnI GCTCC 5 cut(s) 310, 313, 536, 715, 781
Lsp1109I GCAGC 2 cut(s) 363, 464
LweI GCATC 1 cut(s) 141
MaeI CTAG 1 cut(s) 20
MaeII ACGT 4 cut(s) 773, 819, 866, 951
MaeIII GTNAC 2 cut(s) 149, 820
MalI GATC 5 cut(s) 218, 282, 287, 464, 486
MboI GATC 5 cut(s) 216, 280, 285, 462, 484
MboII GAAGA 3 cut(s) 668, 847, 1054
MflI RGATCY 3 cut(s) 285, 462, 484
MhlI GDGCHC 3 cut(s) 318, 786, 879
MlsI TGGCCA 2 cut(s) 269, 276
MluCI AATT 9 cut(s) 199, 344, 407, 550, 588, 642, 650, 943, 1052
MluNI TGGCCA 2 cut(s) 269, 276
MlyI GAGTC 1 cut(s) 620
MmeI TCCRAC 3 cut(s) 127, 934, 978
Mox20I TGGCCA 2 cut(s) 269, 276
MroI TCCGGA 3 cut(s) 165, 426, 487
MroXI GAANNNNTTC 1 cut(s) 600
MscI TGGCCA 2 cut(s) 269, 276
Msp20I TGGCCA 2 cut(s) 269, 276
MspA1I CMGCKG 1 cut(s) 394
MspR9I CCNGG 4 cut(s) 146, 147, 278, 612
MvaI CCWGG 2 cut(s) 278, 612
MvnI CGCG 2 cut(s) 123, 137
MwoI GCNNNNNNNGC 4 cut(s) 129, 474, 732, 848
NciI CCSGG 2 cut(s) 146, 147
NdeII GATC 5 cut(s) 216, 280, 285, 462, 484
NlaIII CATG 9 cut(s) 163, 268, 664, 766, 792, 802, 841, 934, 984
NlaIV GGNNCC 7 cut(s) 173, 287, 306, 315, 486, 532, 804
NmuCI GTSAC 1 cut(s) 149
NruI TCGCGA 1 cut(s) 123
NspI RCATGY 1 cut(s) 984
NspV TTCGAA 1 cut(s) 885
PagI TCATGA 2 cut(s) 762, 930
PdmI GAANNNNTTC 1 cut(s) 600
PfeI GAWTC 2 cut(s) 759, 1001
PflFI GACNNNGTC 1 cut(s) 187
PfoI TCCNGGA 1 cut(s) 610
PkrI GCNGC 2 cut(s) 378, 479
PleI GAGTC 1 cut(s) 620
PpsI GAGTC 1 cut(s) 620
Psp124BI GAGCTC 1 cut(s) 786
Psp6I CCWGG 2 cut(s) 276, 610
PspFI CCCAGC 3 cut(s) 24, 390, 435
PspGI CCWGG 2 cut(s) 276, 610
PspN4I GGNNCC 7 cut(s) 173, 287, 306, 315, 486, 532, 804
PspPI GGNCC 2 cut(s) 94, 388
PstI CTGCAG 1 cut(s) 470
PstNI CAGNNNCTG 2 cut(s) 31, 72
PsuI RGATCY 3 cut(s) 285, 462, 484
PsyI GACNNNGTC 1 cut(s) 187
PvuII CAGCTG 1 cut(s) 394
RruI TCGCGA 1 cut(s) 123
RsaI GTAC 2 cut(s) 992, 1065
RsaNI GTAC 2 cut(s) 991, 1064
SacI GAGCTC 1 cut(s) 786
SalI GTCGAC 1 cut(s) 52
SatI GCNGC 2 cut(s) 377, 478
Sau3AI GATC 5 cut(s) 216, 280, 285, 462, 484
Sau96I GGNCC 2 cut(s) 94, 388
SchI GAGTC 1 cut(s) 620
ScrFI CCNGG 4 cut(s) 146, 147, 278, 612
SduI GDGCHC 3 cut(s) 318, 786, 879
SfaNI GCATC 1 cut(s) 141
SfcI CTRYAG 1 cut(s) 466
SfuI TTCGAA 1 cut(s) 885
SinI GGWCC 1 cut(s) 94
SmaI CCCGGG 1 cut(s) 147
SmlI CTYRAG 1 cut(s) 938
SmoI CTYRAG 1 cut(s) 938
Sse9I AATT 9 cut(s) 199, 344, 407, 550, 588, 642, 650, 943, 1052
SsiI CCGC 6 cut(s) 92, 108, 194, 308, 401, 726
SspMI CTAG 1 cut(s) 20
SstI GAGCTC 1 cut(s) 786
StyD4I CCNGG 4 cut(s) 144, 145, 276, 610
StyI CCWWGG 1 cut(s) 45
TaaI ACNGT 2 cut(s) 189, 299
TaiI ACGT 4 cut(s) 776, 822, 869, 954
TaqI TCGA 5 cut(s) 53, 418, 869, 885, 948
TaqII GACCGA 1 cut(s) 702
TasI AATT 9 cut(s) 199, 344, 407, 550, 588, 642, 650, 943, 1052
TatI WGTACW 1 cut(s) 1063
TfiI GAWTC 2 cut(s) 759, 1001
TseFI GTSAC 1 cut(s) 149
TseI GCWGC 2 cut(s) 376, 477
Tsp45I GTSAC 1 cut(s) 149
TspDTI ATGAA 4 cut(s) 751, 826, 947, 993
TspGWI ACGGA 4 cut(s) 374, 523, 544, 978
TspMI CCCGGG 1 cut(s) 145
Tth111I GACNNNGTC 1 cut(s) 187
VpaK11BI GGWCC 1 cut(s) 94
XapI RAATTY 3 cut(s) 199, 550, 943
XceI RCATGY 1 cut(s) 984
XmaI CCCGGG 1 cut(s) 145
XmiI GTMKAC 1 cut(s) 53
XmnI GAANNNNTTC 1 cut(s) 600
XspI CTAG 1 cut(s) 20
ZraI GACGTC 2 cut(s) 867, 952
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.