FvH4_1g14090

Mitochondrial calcium uniporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0011209
Physical Location & Seq
Forward (+)
7789691 .. 7792515
2825 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g14090.t1

Sequence Viewer

Length: 1059 bp
ATGGTAGTAGTTATGGCGTTCAAGAAAACCCTAGCTGAGCGCCTCTTCAATATCTCAAAGATCTCGACCCAGGCCTTGAGAAATTGCCGGATTTCCTCGTCGGCGGTGCATCTCCGGCCGTCGGCGAAGCCTCGGGAGTCGAGCGAGCCGGGGGACAATAATGCCGTTTTCCGGCGGTTCCTCCACGAGAGAGCCTCCGCCGCGGAGATCTGGTCGGTTCCGGTCGGCGAGAACCTGATGGAGAAGCTCAGGTCCGCCGCCATTTCCCGTAGCCGGATTAGGCTGGACGGTTTGGCTCCGCCGGCGACGGAGAGGGTGTCTGCGCCGGCGGTGATGGCCGGGTTGACGGCGGAGGAGACGAGGAAGCTGCTGAGGGCGGCGCAAATGGAGGCGGTGAAGGCGAAGCTCCGGGAGGTGGAGAAGACGTGGGTGACGTATTCGGAGTTTGTTAGGATTTGCACGGAGGTTTGTTCGGATCCGGATCAGGGTCTCGGGTTTGCGAAATCGCTGGATGAGAGTGGCGCCGTTATCGTTTTGGGGAAGGGCGTTTGCGTCAAACCTGAACAGGTAGCAAAAGCAATCCAAGACCTCATTCCCATCCCTGGAGCCACTCCAAGCGATGCAAAAATGAGGGAGCTAGAAGACATGGAGAAACAGAAATCCATAATAGACCAGAAAGCTGATGCCTTGGTGCGCAGAGAACTATGGTTGGGGCTTGGCTATCTCGTTGTCCAAACAGCCGGGTTCATGAGGCTGACCTTTTGGGAACTCTCATGGGATGTGATGGAACCCATCTGCTTCTATGTGACTTCAATGTACTTCATGGCTTGTTATGCCTTCTTCCTCAGGACCTCCAAAGAGCCCTCTTTCGAAGGCTTCTTCCAGAGCCGTTTTGTTGCCAGGCAGAAGAAGTTGATGAAGGATCACAATTTCGATTTGGGTAGGTATGAGGAGCTCAGAAAAGCTTGTTACTCGCTCTCATCTTCTCCATCGGATCCATCAGCTGCTTTTGATGGAGCTAAGAGGATGCCAATGCAGTTTGGTTCCAATGTTCTATAG

Protein Analysis

353

Amino Acids

39.46

Weight (kDa)

9.07

Isoelectric Point (pI)

49.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MCU PF04678 161 - 319 1.4e-51 Mitochondrial calcium uniporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011209)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23790 AT4G36820 AT5G66650
fragaria_vesca FvH4_1g14090
malus_domestica MD02G1155000.v1.1 MD15G1269400.v1.1 MD15G1269500.v1.1
prunus_persica Prupe.7G148300_v2.0.a1
pyrus_communis pycom02g12270 pycom15g23380
rosa_chinensis RchiOBHm_Chr2g0102751
rosa_laevigata RLG00000017177
rosa_multiflora Rmu_co8164376.1_g000001
rosa_roxburghii Rroxscaffold_2G00140340
rosa_rugosa Rorug02G0109400
rosa_samantha Rh2AG157700 Rh2BG164200 Rh2CG163900 Rh2DG163000
rosa_wichuraiana Rw2G012230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 695
AccB1I GGYRCC 1 cut(s) 521
AccII CGCG 1 cut(s) 203
AccIII TCCGGA 1 cut(s) 478
AclWI GGATC 6 cut(s) 470, 483, 489, 930, 989, 1002
AcoI YGGCCR 2 cut(s) 116, 336
AcyI GRCGYC 1 cut(s) 522
AfaI GTAC 1 cut(s) 818
AfiI CCNNNNNNNGG 6 cut(s) 121, 171, 273, 415, 485, 602
AgsI TTSAA 3 cut(s) 22, 49, 813
AjiI CACGTC 1 cut(s) 426
AjnI CCWGG 3 cut(s) 69, 601, 899
AjuI GAANNNNNNNTTGG 2 cut(s) 576, 608
Alw21I GWGCWC 1 cut(s) 957
Alw26I GTCTC 2 cut(s) 350, 494
AlwI GGATC 6 cut(s) 470, 483, 489, 930, 989, 1002
Ama87I CYCGRG 2 cut(s) 132, 491
Aor13HI TCCGGA 1 cut(s) 478
AoxI GGCC 3 cut(s) 72, 116, 336
ApeKI GCWGC 2 cut(s) 367, 1004
AspLEI GCGC 5 cut(s) 42, 325, 382, 524, 696
AspS9I GGNCC 2 cut(s) 252, 849
AsuC2I CCSGG 4 cut(s) 150, 340, 410, 742
AsuHPI GGTGA 3 cut(s) 343, 406, 442
AsuII TTCGAA 1 cut(s) 870
AvaI CYCGRG 2 cut(s) 132, 491
AvaII GGWCC 2 cut(s) 252, 849
AxyI CCTNAGG 1 cut(s) 845
BamHI GGATCC 2 cut(s) 475, 994
BanI GGYRCC 1 cut(s) 521
BanII GRGCYC 2 cut(s) 864, 957
BauI CACGAG 1 cut(s) 185
BbsI GAAGAC 2 cut(s) 428, 648
Bbv12I GWGCWC 1 cut(s) 957
BbvCI CCTCAGC 1 cut(s) 371
BbvI GCAGC 2 cut(s) 354, 991
BccI CCATC 8 cut(s) 232, 328, 605, 778, 800, 997, 1006, 1007
BceAI ACGGC 5 cut(s) 103, 149, 363, 509, 873
BcgI CGANNNNNNTGC 2 cut(s) 349, 383
BciT130I CCWGG 3 cut(s) 71, 603, 901
BcnI CCSGG 4 cut(s) 150, 340, 410, 742
BcoDI GTCTC 2 cut(s) 350, 494
BfaI CTAG 2 cut(s) 32, 638
BfmI CTRYAG 1 cut(s) 1055
BfoI RGCGCY 2 cut(s) 43, 525
BglII AGATCT 2 cut(s) 60, 207
BisI GCNGC 5 cut(s) 201, 258, 368, 378, 1005
BlpI GCTNAGC 1 cut(s) 36
BlsI GCNGC 5 cut(s) 202, 259, 369, 379, 1006
Bme1390I CCNGG 7 cut(s) 71, 150, 340, 410, 603, 742, 901
Bme18I GGWCC 2 cut(s) 252, 849
BmeT110I CYCGRG 2 cut(s) 132, 491
BmgBI CACGTC 1 cut(s) 426
BmgT120I GGNCC 2 cut(s) 252, 849
BmiI GGNNCC 9 cut(s) 179, 219, 297, 477, 523, 607, 789, 996, 1045
BmrFI CCNGG 7 cut(s) 71, 150, 340, 410, 603, 742, 901
BmsI GCATC 4 cut(s) 118, 610, 673, 1017
BpiI GAAGAC 2 cut(s) 428, 648
BplI GAGNNNNNCTC 2 cut(s) 179, 211
BpmI CTGGAG 1 cut(s) 624
Bpu10I CCTNAGC 2 cut(s) 248, 371
Bpu1102I GCTNAGC 1 cut(s) 36
Bpu14I TTCGAA 1 cut(s) 870
BpuEI CTTGAG 1 cut(s) 97
BpuMI CCSGG 4 cut(s) 150, 340, 410, 742
BsaBI GATNNNNATC 1 cut(s) 480
BsaHI GRCGYC 1 cut(s) 522
BsaI GGTCTC 1 cut(s) 494
BsaJI CCNNGG 6 cut(s) 69, 131, 149, 201, 601, 687
BsaWI WCCGGW 2 cut(s) 220, 478
BsaXI ACNNNNNCTCC 6 cut(s) 197, 227, 302, 332, 410, 440
Bsc4I CCNNNNNNNGG 6 cut(s) 121, 171, 273, 415, 485, 602
Bse118I RCCGGY 2 cut(s) 301, 325
Bse21I CCTNAGG 1 cut(s) 845
Bse8I GATNNNNATC 1 cut(s) 480
BseAI TCCGGA 1 cut(s) 478
BseBI CCWGG 3 cut(s) 71, 603, 901
BseDI CCNNGG 6 cut(s) 69, 131, 149, 201, 601, 687
BseGI GGATG 4 cut(s) 517, 597, 784, 1032
BseJI GATNNNNATC 1 cut(s) 480
BseLI CCNNNNNNNGG 6 cut(s) 121, 171, 273, 415, 485, 602
BseMII CTCAG 5 cut(s) 27, 262, 362, 859, 970
BseRI GAGGAG 2 cut(s) 368, 965
BseX3I CGGCCG 1 cut(s) 116
BseXI GCAGC 2 cut(s) 354, 991
Bsh1236I CGCG 1 cut(s) 203
Bsh1285I CGRYCG 2 cut(s) 119, 225
BshFI GGCC 3 cut(s) 74, 118, 338
BshNI GGYRCC 1 cut(s) 521
BsiEI CGRYCG 2 cut(s) 119, 225
BsiHKAI GWGCWC 1 cut(s) 957
BsiHKCI CYCGRG 2 cut(s) 132, 491
BslFI GGGAC 1 cut(s) 167
BslI CCNNNNNNNGG 6 cut(s) 121, 171, 273, 415, 485, 602
BsmAI GTCTC 2 cut(s) 350, 494
BsmBI CGTCTC 1 cut(s) 350
BsmFI GGGAC 1 cut(s) 167
BsnI GGCC 3 cut(s) 74, 118, 338
Bso31I GGTCTC 1 cut(s) 494
BsoBI CYCGRG 2 cut(s) 132, 491
Bsp119I TTCGAA 1 cut(s) 870
Bsp1286I GDGCHC 2 cut(s) 864, 957
Bsp13I TCCGGA 1 cut(s) 478
Bsp143I GATC 6 cut(s) 60, 207, 475, 481, 922, 994
Bsp1720I GCTNAGC 1 cut(s) 36
BspANI GGCC 3 cut(s) 74, 118, 338
BspCNI CTCAG 5 cut(s) 28, 261, 363, 858, 969
BspEI TCCGGA 1 cut(s) 478
BspFNI CGCG 1 cut(s) 203
BspHI TCATGA 1 cut(s) 747
BspLI GGNNCC 9 cut(s) 179, 219, 297, 477, 523, 607, 789, 996, 1045
BspPI GGATC 6 cut(s) 470, 483, 489, 930, 989, 1002
BspT104I TTCGAA 1 cut(s) 870
BspT107I GGYRCC 1 cut(s) 521
BspTNI GGTCTC 1 cut(s) 494
BsrFI RCCGGY 2 cut(s) 301, 325
BssAI RCCGGY 2 cut(s) 301, 325
BssECI CCNNGG 6 cut(s) 69, 131, 149, 201, 601, 687
BssMI GATC 6 cut(s) 60, 207, 475, 481, 922, 994
BssNI GRCGYC 1 cut(s) 522
BssSI CACGAG 1 cut(s) 185
BssT1I CCWWGG 1 cut(s) 687
Bst2BI CACGAG 1 cut(s) 185
Bst2UI CCWGG 3 cut(s) 71, 603, 901
Bst4CI ACNGT 1 cut(s) 290
Bst6I CTCTTC 1 cut(s) 50
BstACI GRCGYC 1 cut(s) 522
BstBI TTCGAA 1 cut(s) 870
BstC8I GCNNGC 3 cut(s) 146, 303, 327
BstDEI CTNAG 6 cut(s) 36, 248, 371, 845, 956, 1020
BstDSI CCRYGG 1 cut(s) 201
BstF5I GGATG 4 cut(s) 517, 597, 784, 1032
BstFNI CGCG 1 cut(s) 203
BstH2I RGCGCY 2 cut(s) 43, 525
BstHHI GCGC 5 cut(s) 42, 325, 382, 524, 696
BstKTI GATC 6 cut(s) 63, 210, 478, 484, 925, 997
BstMAI GTCTC 2 cut(s) 350, 494
BstMBI GATC 6 cut(s) 60, 207, 475, 481, 922, 994
BstMCI CGRYCG 2 cut(s) 119, 225
BstMWI GCNNNNNNNGC 6 cut(s) 115, 200, 302, 335, 398, 833
BstNI CCWGG 3 cut(s) 71, 603, 901
BstSCI CCNGG 7 cut(s) 69, 148, 338, 408, 601, 740, 899
BstSFI CTRYAG 1 cut(s) 1055
BstUI CGCG 1 cut(s) 203
BstV1I GCAGC 2 cut(s) 354, 991
BstV2I GAAGAC 2 cut(s) 428, 648
BstX2I RGATCY 4 cut(s) 60, 207, 475, 994
BstYI RGATCY 4 cut(s) 60, 207, 475, 994
BstZI CGGCCG 1 cut(s) 116
Bsu36I CCTNAGG 1 cut(s) 845
BsuRI GGCC 3 cut(s) 74, 118, 338
BtgI CCRYGG 1 cut(s) 201
BtgZI GCGATG 1 cut(s) 633
BtrI CACGTC 1 cut(s) 426
BtsCI GGATG 4 cut(s) 517, 597, 784, 1032
Cac8I GCNNGC 3 cut(s) 146, 303, 327
CciI TCATGA 1 cut(s) 747
CfoI GCGC 5 cut(s) 42, 325, 382, 524, 696
Cfr10I RCCGGY 2 cut(s) 301, 325
Cfr13I GGNCC 2 cut(s) 252, 849
Cfr42I CCGCGG 1 cut(s) 204
CseI GACGC 1 cut(s) 541
Csp6I GTAC 1 cut(s) 817
CviAII CATG 4 cut(s) 646, 748, 774, 823
CviQI GTAC 1 cut(s) 817
DdeI CTNAG 6 cut(s) 36, 248, 371, 845, 956, 1020
DinI GGCGCC 1 cut(s) 523
DpnI GATC 6 cut(s) 62, 209, 477, 483, 924, 996
DpnII GATC 6 cut(s) 60, 207, 475, 481, 922, 994
EaeI YGGCCR 2 cut(s) 116, 336
EagI CGGCCG 1 cut(s) 116
Eam1104I CTCTTC 1 cut(s) 50
EarI CTCTTC 1 cut(s) 50
EciI GGCGGA 4 cut(s) 187, 244, 288, 365
Ecl136II GAGCTC 1 cut(s) 955
EclXI CGGCCG 1 cut(s) 116
Eco130I CCWWGG 1 cut(s) 687
Eco147I AGGCCT 1 cut(s) 74
Eco24I GRGCYC 2 cut(s) 864, 957
Eco31I GGTCTC 1 cut(s) 494
Eco47I GGWCC 2 cut(s) 252, 849
Eco52I CGGCCG 1 cut(s) 116
Eco53kI GAGCTC 1 cut(s) 955
Eco81I CCTNAGG 1 cut(s) 845
Eco88I CYCGRG 2 cut(s) 132, 491
EcoICRI GAGCTC 1 cut(s) 955
EcoO109I RGGNCCY 1 cut(s) 849
EcoRII CCWGG 3 cut(s) 69, 601, 899
EcoT14I CCWWGG 1 cut(s) 687
EcoT38I GRGCYC 2 cut(s) 864, 957
EgeI GGCGCC 1 cut(s) 523
EheI GGCGCC 1 cut(s) 523
ErhI CCWWGG 1 cut(s) 687
Esp3I CGTCTC 1 cut(s) 350
FaeI CATG 4 cut(s) 649, 751, 777, 826
FaqI GGGAC 1 cut(s) 167
FatI CATG 4 cut(s) 645, 747, 773, 822
Fnu4HI GCNGC 5 cut(s) 201, 258, 368, 378, 1005
FokI GGATG 4 cut(s) 524, 584, 791, 1039
FriOI GRGCYC 2 cut(s) 864, 957
Fsp4HI GCNGC 5 cut(s) 201, 258, 368, 378, 1005
FspBI CTAG 2 cut(s) 32, 638
FspI TGCGCA 1 cut(s) 695
GlaI GCGC 5 cut(s) 41, 324, 381, 523, 695
GluI GCNGC 5 cut(s) 201, 258, 368, 378, 1005
GsuI CTGGAG 1 cut(s) 624
HaeII RGCGCY 2 cut(s) 43, 525
HaeIII GGCC 3 cut(s) 74, 118, 338
HgaI GACGC 1 cut(s) 541
HhaI GCGC 5 cut(s) 42, 325, 382, 524, 696
Hin1I GRCGYC 1 cut(s) 522
Hin1II CATG 4 cut(s) 649, 751, 777, 826
Hin6I GCGC 5 cut(s) 40, 323, 380, 522, 694
HinP1I GCGC 5 cut(s) 40, 323, 380, 522, 694
HincII GTYRAC 1 cut(s) 345
HindII GTYRAC 1 cut(s) 345
HindIII AAGCTT 1 cut(s) 963
HinfI GANTC 1 cut(s) 137
HphI GGTGA 3 cut(s) 343, 406, 442
Hpy166II GTNNAC 1 cut(s) 345
Hpy188I TCNGA 4 cut(s) 442, 475, 959, 994
Hpy188III TCNNGA 7 cut(s) 22, 64, 134, 479, 748, 847, 883
Hpy8I GTNNAC 1 cut(s) 345
Hpy99I CGWCG 3 cut(s) 103, 124, 310
HpyAV CCTTC 5 cut(s) 391, 535, 847, 866, 913
HpyCH4III ACNGT 1 cut(s) 290
HpyCH4IV ACGT 2 cut(s) 425, 434
HpyCH4V TGCA 4 cut(s) 109, 459, 623, 1036
HpyF10VI GCNNNNNNNGC 6 cut(s) 115, 200, 302, 335, 398, 833
HpyF3I CTNAG 6 cut(s) 36, 248, 371, 845, 956, 1020
HpySE526I ACGT 2 cut(s) 425, 434
Hsp92I GRCGYC 1 cut(s) 522
Hsp92II CATG 4 cut(s) 649, 751, 777, 826
HspAI GCGC 5 cut(s) 40, 323, 380, 522, 694
KasI GGCGCC 1 cut(s) 521
Kpn2I TCCGGA 1 cut(s) 478
KroI GCCGGC 2 cut(s) 301, 325
KroNI GCCGGC 2 cut(s) 303, 327
KspI CCGCGG 1 cut(s) 204
Kzo9I GATC 6 cut(s) 60, 207, 475, 481, 922, 994
LmnI GCTCC 6 cut(s) 301, 411, 605, 634, 952, 1016
Lsp1109I GCAGC 2 cut(s) 354, 991
LweI GCATC 4 cut(s) 118, 610, 673, 1017
MaeI CTAG 2 cut(s) 32, 638
MaeII ACGT 2 cut(s) 425, 434
MaeIII GTNAC 3 cut(s) 430, 805, 968
MalI GATC 6 cut(s) 62, 209, 477, 483, 924, 996
MboI GATC 6 cut(s) 60, 207, 475, 481, 922, 994
MboII GAAGA 7 cut(s) 37, 433, 653, 832, 871, 919, 975
MflI RGATCY 4 cut(s) 60, 207, 475, 994
MhlI GDGCHC 2 cut(s) 864, 957
MluCI AATT 2 cut(s) 82, 928
Mly113I GGCGCC 1 cut(s) 522
MlyI GAGTC 1 cut(s) 146
MreI CGCCGGCG 2 cut(s) 301, 325
MroI TCCGGA 1 cut(s) 478
MroNI GCCGGC 2 cut(s) 301, 325
MspA1I CMGCKG 2 cut(s) 203, 1004
MspR9I CCNGG 7 cut(s) 71, 150, 340, 410, 603, 742, 901
MvaI CCWGG 3 cut(s) 71, 603, 901
MvnI CGCG 1 cut(s) 203
MwoI GCNNNNNNNGC 6 cut(s) 115, 200, 302, 335, 398, 833
NaeI GCCGGC 2 cut(s) 303, 327
NarI GGCGCC 1 cut(s) 522
NciI CCSGG 4 cut(s) 150, 340, 410, 742
NdeII GATC 6 cut(s) 60, 207, 475, 481, 922, 994
NgoMIV GCCGGC 2 cut(s) 301, 325
NlaIII CATG 4 cut(s) 649, 751, 777, 826
NlaIV GGNNCC 9 cut(s) 179, 219, 297, 477, 523, 607, 789, 996, 1045
NmuCI GTSAC 2 cut(s) 430, 805
NsbI TGCGCA 1 cut(s) 695
NspV TTCGAA 1 cut(s) 870
PagI TCATGA 1 cut(s) 747
PceI AGGCCT 1 cut(s) 74
PcsI WCGNNNNNNNCGW 1 cut(s) 431
PdiI GCCGGC 2 cut(s) 303, 327
PfoI TCCNGGA 1 cut(s) 408
PkrI GCNGC 5 cut(s) 202, 259, 369, 379, 1006
PleI GAGTC 1 cut(s) 145
PluTI GGCGCC 1 cut(s) 525
PpsI GAGTC 1 cut(s) 145
PpuMI RGGWCCY 1 cut(s) 849
Psp124BI GAGCTC 1 cut(s) 957
Psp5II RGGWCCY 1 cut(s) 849
Psp6I CCWGG 3 cut(s) 69, 601, 899
PspGI CCWGG 3 cut(s) 69, 601, 899
PspN4I GGNNCC 9 cut(s) 179, 219, 297, 477, 523, 607, 789, 996, 1045
PspPI GGNCC 2 cut(s) 252, 849
PspPPI RGGWCCY 1 cut(s) 849
PsuI RGATCY 4 cut(s) 60, 207, 475, 994
PvuII CAGCTG 1 cut(s) 1004
RsaI GTAC 1 cut(s) 818
RsaNI GTAC 1 cut(s) 817
SacI GAGCTC 1 cut(s) 957
SacII CCGCGG 1 cut(s) 204
SatI GCNGC 5 cut(s) 201, 258, 368, 378, 1005
Sau3AI GATC 6 cut(s) 60, 207, 475, 481, 922, 994
Sau96I GGNCC 2 cut(s) 252, 849
SchI GAGTC 1 cut(s) 146
ScrFI CCNGG 7 cut(s) 71, 150, 340, 410, 603, 742, 901
SduI GDGCHC 2 cut(s) 864, 957
SfaNI GCATC 4 cut(s) 118, 610, 673, 1017
SfcI CTRYAG 1 cut(s) 1055
SfoI GGCGCC 1 cut(s) 523
Sfr303I CCGCGG 1 cut(s) 204
SfuI TTCGAA 1 cut(s) 870
SgrAI CRCCGGYG 2 cut(s) 301, 325
SgrBI CCGCGG 1 cut(s) 204
SinI GGWCC 2 cut(s) 252, 849
SmlI CTYRAG 1 cut(s) 76
SmoI CTYRAG 1 cut(s) 76
Sse9I AATT 2 cut(s) 82, 928
SseBI AGGCCT 1 cut(s) 74
SspDI GGCGCC 1 cut(s) 521
SspMI CTAG 2 cut(s) 32, 638
SstI GAGCTC 1 cut(s) 957
StuI AGGCCT 1 cut(s) 74
StyD4I CCNGG 7 cut(s) 69, 148, 338, 408, 601, 740, 899
StyI CCWWGG 1 cut(s) 687
TaaI ACNGT 1 cut(s) 290
TaiI ACGT 2 cut(s) 428, 437
TaqI TCGA 4 cut(s) 65, 140, 870, 933
TasI AATT 2 cut(s) 82, 928
TatI WGTACW 1 cut(s) 816
TauI GCSGC 3 cut(s) 203, 260, 380
TseFI GTSAC 2 cut(s) 430, 805
TseI GCWGC 2 cut(s) 367, 1004
Tsp45I GTSAC 2 cut(s) 430, 805
TspDTI ATGAA 3 cut(s) 736, 811, 932
TspGWI ACGGA 2 cut(s) 323, 476
VpaK11BI GGWCC 2 cut(s) 252, 849
XspI CTAG 2 cut(s) 32, 638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.