MD15G1269500.v1.1

Mitochondrial calcium uniporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0011209
Physical Location & Seq
Forward (+)
23290694 .. 23292029
1336 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1269500.v1.1.491

Sequence Viewer

Length: 678 bp
ATGTTTGAATTTGGTATTTGGTATGCATACCTAATTAGGTATGGGGTTATCCATTTTGTTCCCATATCTTTCTCTATCTTTTTGCTTGCATATCTCATCAACTTAATGGATCAGGCTACAATACACCGGAGTATGTATGGCAATATTCCATCATTTAGAGCCGTTAGATCTAATTCCGGTATAGCCAAAGCAATCCAGGAACTAATTCCTATTCCAGGAACAAGAACCAACTCCAATGATCCCAGGATTAGGGAATTGGAAGACATGGAGAGGCACAAGTTTGAGATAGACCGAAAGGCGGAGTCCCTTGTTCGAAAGGAGCTTTTGTGCGGTCTTGGCTATTTGGTTGTCTCCACAGCCGGATTCATGAGGCTCACATTCTGGGAACTCTCATGGGACGTCATGGAACCCATTTGCTTCTTCGTTACTTCAATGTACTTCATAGCTGGATACGCCTTCTTTCTCAGGACCCGGAAAGAGCCCTCTTTCGAAGGGTTTCGCCAGATTCGATTTGAGGCTAAGCAGAAGCGCCTCATGAAGCTTGAGAACTTCGATGTCGGAAGGTATGACGAGCTTCGTAAAGCATGTTGTCCCTACTCATCGGCTTCATCAGAAATGACCCTGTCAACTGCTTTTGATGGGGCAAAGAGGATGCAATTTGGTTCACTACATAGTTAA

Protein Analysis

226

Amino Acids

26.21

Weight (kDa)

9.03

Isoelectric Point (pI)

36.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MCU PF04678 77 - 192 9.2e-46 Mitochondrial calcium uniporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011209)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23790 AT4G36820 AT5G66650
fragaria_vesca FvH4_1g14090
malus_domestica MD02G1155000.v1.1 MD15G1269400.v1.1 MD15G1269500.v1.1
prunus_persica Prupe.7G148300_v2.0.a1
pyrus_communis pycom02g12270 pycom15g23380
rosa_chinensis RchiOBHm_Chr2g0102751
rosa_laevigata RLG00000017177
rosa_multiflora Rmu_co8164376.1_g000001
rosa_roxburghii Rroxscaffold_2G00140340
rosa_rugosa Rorug02G0109400
rosa_samantha Rh2AG157700 Rh2BG164200 Rh2CG163900 Rh2DG163000
rosa_wichuraiana Rw2G012230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 402
AciI CCGC 2 cut(s) 299, 330
AclWI GGATC 2 cut(s) 117, 233
AcsI RAATTY 1 cut(s) 8
AcyI GRCGYC 1 cut(s) 399
AfaI GTAC 1 cut(s) 437
AfiI CCNNNNNNNGG 3 cut(s) 215, 249, 298
AgsI TTSAA 2 cut(s) 8, 432
AjnI CCWGG 3 cut(s) 195, 214, 242
AluBI AGCT 4 cut(s) 322, 446, 541, 574
AluI AGCT 4 cut(s) 322, 446, 541, 574
Alw26I GTCTC 1 cut(s) 355
AlwI GGATC 2 cut(s) 117, 233
ApoI RAATTY 1 cut(s) 8
Asp700I GAANNNNTTC 2 cut(s) 204, 495
AspLEI GCGC 1 cut(s) 531
AspS9I GGNCC 1 cut(s) 468
AsuC2I CCSGG 1 cut(s) 472
AsuII TTCGAA 2 cut(s) 313, 489
AvaII GGWCC 1 cut(s) 468
BanII GRGCYC 1 cut(s) 483
BbsI GAAGAC 1 cut(s) 267
BccI CCATC 2 cut(s) 157, 632
BceAI ACGGC 1 cut(s) 146
BciT130I CCWGG 3 cut(s) 197, 216, 244
BciVI GTATCC 1 cut(s) 443
BcnI CCSGG 1 cut(s) 472
BcoDI GTCTC 1 cut(s) 355
BfoI RGCGCY 1 cut(s) 532
BfuI GTATCC 1 cut(s) 443
BglII AGATCT 1 cut(s) 167
BlpI GCTNAGC 1 cut(s) 519
Bme1390I CCNGG 4 cut(s) 197, 216, 244, 472
Bme18I GGWCC 1 cut(s) 468
BmgT120I GGNCC 1 cut(s) 468
BmiI GGNNCC 2 cut(s) 408, 470
BmrFI CCNGG 4 cut(s) 197, 216, 244, 472
BmsI GCATC 1 cut(s) 642
BpiI GAAGAC 1 cut(s) 267
Bpu1102I GCTNAGC 1 cut(s) 519
Bpu14I TTCGAA 2 cut(s) 313, 489
BpuEI CTTGAG 1 cut(s) 563
BpuMI CCSGG 1 cut(s) 472
BsaHI GRCGYC 1 cut(s) 399
BsaJI CCNNGG 1 cut(s) 242
BsaWI WCCGGW 2 cut(s) 126, 176
BsaXI ACNNNNNCTCC 2 cut(s) 311, 341
Bsc4I CCNNNNNNNGG 3 cut(s) 215, 249, 298
BseBI CCWGG 3 cut(s) 197, 216, 244
BseDI CCNNGG 1 cut(s) 242
BseGI GGATG 1 cut(s) 657
BseLI CCNNNNNNNGG 3 cut(s) 215, 249, 298
BseMII CTCAG 1 cut(s) 478
BsiSI CCGG 4 cut(s) 127, 177, 360, 472
BslFI GGGAC 3 cut(s) 289, 410, 576
BslI CCNNNNNNNGG 3 cut(s) 215, 249, 298
BsmAI GTCTC 1 cut(s) 355
BsmFI GGGAC 3 cut(s) 289, 410, 576
Bsp119I TTCGAA 2 cut(s) 313, 489
Bsp1286I GDGCHC 1 cut(s) 483
Bsp143I GATC 3 cut(s) 109, 167, 238
Bsp1720I GCTNAGC 1 cut(s) 519
BspACI CCGC 2 cut(s) 299, 330
BspCNI CTCAG 1 cut(s) 477
BspHI TCATGA 2 cut(s) 366, 534
BspLI GGNNCC 2 cut(s) 408, 470
BspPI GGATC 2 cut(s) 117, 233
BspT104I TTCGAA 2 cut(s) 313, 489
BssECI CCNNGG 1 cut(s) 242
BssMI GATC 3 cut(s) 109, 167, 238
BssNI GRCGYC 1 cut(s) 399
Bst2UI CCWGG 3 cut(s) 197, 216, 244
BstACI GRCGYC 1 cut(s) 399
BstBI TTCGAA 2 cut(s) 313, 489
BstC8I GCNNGC 1 cut(s) 87
BstDEI CTNAG 2 cut(s) 464, 519
BstENI CCTNNNNNAGG 1 cut(s) 213
BstF5I GGATG 1 cut(s) 657
BstH2I RGCGCY 1 cut(s) 532
BstHHI GCGC 1 cut(s) 531
BstKTI GATC 3 cut(s) 112, 170, 241
BstMAI GTCTC 1 cut(s) 355
BstMBI GATC 3 cut(s) 109, 167, 238
BstMWI GCNNNNNNNGC 2 cut(s) 336, 452
BstNI CCWGG 3 cut(s) 197, 216, 244
BstNSI RCATGY 1 cut(s) 588
BstSCI CCNGG 4 cut(s) 195, 214, 242, 470
BstV2I GAAGAC 1 cut(s) 267
BstX2I RGATCY 1 cut(s) 167
BstYI RGATCY 1 cut(s) 167
BsuI GTATCC 1 cut(s) 443
BtsCI GGATG 1 cut(s) 657
Cac8I GCNNGC 1 cut(s) 87
CciI TCATGA 2 cut(s) 366, 534
CfoI GCGC 1 cut(s) 531
Cfr13I GGNCC 1 cut(s) 468
Csp6I GTAC 1 cut(s) 436
CviAII CATG 6 cut(s) 265, 367, 393, 403, 535, 585
CviQI GTAC 1 cut(s) 436
DdeI CTNAG 2 cut(s) 464, 519
DpnI GATC 3 cut(s) 111, 169, 240
DpnII GATC 3 cut(s) 109, 167, 238
EciI GGCGGA 1 cut(s) 314
Eco24I GRGCYC 1 cut(s) 483
Eco47I GGWCC 1 cut(s) 468
EcoNI CCTNNNNNAGG 1 cut(s) 213
EcoO109I RGGNCCY 1 cut(s) 468
EcoRII CCWGG 3 cut(s) 195, 214, 242
EcoT22I ATGCAT 1 cut(s) 28
EcoT38I GRGCYC 1 cut(s) 483
FaeI CATG 6 cut(s) 268, 370, 396, 406, 538, 588
FaqI GGGAC 3 cut(s) 289, 410, 576
FatI CATG 6 cut(s) 264, 366, 392, 402, 534, 584
FokI GGATG 1 cut(s) 664
FriOI GRGCYC 1 cut(s) 483
GlaI GCGC 1 cut(s) 530
HaeII RGCGCY 1 cut(s) 532
HapII CCGG 4 cut(s) 127, 177, 360, 472
HhaI GCGC 1 cut(s) 531
Hin1I GRCGYC 1 cut(s) 399
Hin1II CATG 6 cut(s) 268, 370, 396, 406, 538, 588
Hin6I GCGC 1 cut(s) 529
HinP1I GCGC 1 cut(s) 529
HincII GTYRAC 1 cut(s) 627
HindII GTYRAC 1 cut(s) 627
HindIII AAGCTT 1 cut(s) 539
HinfI GANTC 3 cut(s) 302, 363, 505
HpaII CCGG 4 cut(s) 127, 177, 360, 472
Hpy166II GTNNAC 2 cut(s) 627, 665
Hpy188I TCNGA 2 cut(s) 560, 613
Hpy188III TCNNGA 3 cut(s) 367, 466, 535
Hpy8I GTNNAC 2 cut(s) 627, 665
HpyAV CCTTC 3 cut(s) 466, 485, 555
HpyCH4IV ACGT 1 cut(s) 399
HpyCH4V TGCA 3 cut(s) 26, 89, 655
HpyF10VI GCNNNNNNNGC 2 cut(s) 336, 452
HpyF3I CTNAG 2 cut(s) 464, 519
HpySE526I ACGT 1 cut(s) 399
Hsp92I GRCGYC 1 cut(s) 399
Hsp92II CATG 6 cut(s) 268, 370, 396, 406, 538, 588
HspAI GCGC 1 cut(s) 529
Kzo9I GATC 3 cut(s) 109, 167, 238
LmnI GCTCC 1 cut(s) 319
LweI GCATC 1 cut(s) 642
MaeII ACGT 1 cut(s) 399
MaeIII GTNAC 1 cut(s) 424
MalI GATC 3 cut(s) 111, 169, 240
MboI GATC 3 cut(s) 109, 167, 238
MboII GAAGA 2 cut(s) 272, 412
MflI RGATCY 1 cut(s) 167
MhlI GDGCHC 1 cut(s) 483
MluCI AATT 6 cut(s) 8, 33, 172, 204, 254, 656
MlyI GAGTC 1 cut(s) 311
MmeI TCCRAC 1 cut(s) 538
MnlI CCTC 6 cut(s) 264, 363, 493, 508, 542, 642
Mph1103I ATGCAT 1 cut(s) 28
MroXI GAANNNNTTC 2 cut(s) 204, 495
MseI TTAA 2 cut(s) 104, 676
MspI CCGG 4 cut(s) 127, 177, 360, 472
MspR9I CCNGG 4 cut(s) 197, 216, 244, 472
MvaI CCWGG 3 cut(s) 197, 216, 244
MwoI GCNNNNNNNGC 2 cut(s) 336, 452
NciI CCSGG 1 cut(s) 472
NdeII GATC 3 cut(s) 109, 167, 238
NlaIII CATG 6 cut(s) 268, 370, 396, 406, 538, 588
NlaIV GGNNCC 2 cut(s) 408, 470
NsiI ATGCAT 1 cut(s) 28
NspI RCATGY 1 cut(s) 588
NspV TTCGAA 2 cut(s) 313, 489
PagI TCATGA 2 cut(s) 366, 534
PcsI WCGNNNNNNNCGW 1 cut(s) 505
PdmI GAANNNNTTC 2 cut(s) 204, 495
PfeI GAWTC 2 cut(s) 363, 505
PflFI GACNNNGTC 1 cut(s) 622
PfoI TCCNGGA 2 cut(s) 195, 214
PleI GAGTC 1 cut(s) 310
PpsI GAGTC 1 cut(s) 310
PpuMI RGGWCCY 1 cut(s) 468
Psp5II RGGWCCY 1 cut(s) 468
Psp6I CCWGG 3 cut(s) 195, 214, 242
PspGI CCWGG 3 cut(s) 195, 214, 242
PspN4I GGNNCC 2 cut(s) 408, 470
PspPI GGNCC 1 cut(s) 468
PspPPI RGGWCCY 1 cut(s) 468
PsuI RGATCY 1 cut(s) 167
PsyI GACNNNGTC 1 cut(s) 622
RsaI GTAC 1 cut(s) 437
RsaNI GTAC 1 cut(s) 436
SaqAI TTAA 2 cut(s) 104, 676
Sau3AI GATC 3 cut(s) 109, 167, 238
Sau96I GGNCC 1 cut(s) 468
SchI GAGTC 1 cut(s) 311
ScrFI CCNGG 4 cut(s) 197, 216, 244, 472
SduI GDGCHC 1 cut(s) 483
SetI ASST 8 cut(s) 33, 41, 324, 402, 448, 543, 566, 576
SfaNI GCATC 1 cut(s) 642
SfuI TTCGAA 2 cut(s) 313, 489
SinI GGWCC 1 cut(s) 468
SmlI CTYRAG 1 cut(s) 542
SmoI CTYRAG 1 cut(s) 542
Sse9I AATT 6 cut(s) 8, 33, 172, 204, 254, 656
SsiI CCGC 2 cut(s) 299, 330
SspI AATATT 1 cut(s) 145
StyD4I CCNGG 4 cut(s) 195, 214, 242, 470
TaiI ACGT 1 cut(s) 402
TaqI TCGA 4 cut(s) 313, 489, 508, 552
TaqII GACCGA 1 cut(s) 306
TasI AATT 6 cut(s) 8, 33, 172, 204, 254, 656
TatI WGTACW 1 cut(s) 435
TfiI GAWTC 2 cut(s) 363, 505
Tru1I TTAA 2 cut(s) 104, 676
Tru9I TTAA 2 cut(s) 104, 676
TspDTI ATGAA 4 cut(s) 355, 430, 551, 597
Tth111I GACNNNGTC 1 cut(s) 622
VpaK11BI GGWCC 1 cut(s) 468
XagI CCTNNNNNAGG 1 cut(s) 213
XapI RAATTY 1 cut(s) 8
XceI RCATGY 1 cut(s) 588
XmnI GAANNNNTTC 2 cut(s) 204, 495
ZraI GACGTC 1 cut(s) 400
Zsp2I ATGCAT 1 cut(s) 28
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.