RLG00000017177

Mitochondrial calcium uniporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0011209
Physical Location & Seq
Forward (+)
13784805 .. 13786985
2181 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017177

Sequence Viewer

Length: 1080 bp
ATGGCGTTCAAGAAAACCCTAGCTGAGCGCATCTTCAATATCTCCAAGATCTCGACCCAAACCCTCAGAAACTGCCGGATTTCCTCGTCGGCGGTGCATCTCCGGCCGGCGGCGAAGCCTCGGGAGGCGAGCATCGCTCCCGAGCCGGGAGACAATGCCATTTTCCGGCGGTTCATTCACAGGAGGGCCGGCGTCGCGAAGCCCGAGATATGGTCGGTCCCGATCGGCGAGAACCTGATGGAAAAGCTCAGGTCCGTCGGCATTCCCGGAAGCCGGATTCGGTTGGACGGTTTGGCCCCGCCGGCGCCGGTGACGGAGACGGAGAAGGTGTCTACGCCGGCTGTCATGGCCGGACTGACGGCGGAGGAGACGAGGAAGTTGTTGAGGGTGGCGCAGATGGAGGCGGTGAAGGCGAAACTCCGTGAGGTGCGGAAAACGTGGGTGACGTATTCGGAGTTTGTTGGGATTTGCACGGAGGGTTGTTCGGATCCGGATCTGGGTCTCGGGTTTGCGAAATCGCTGGACGAGAGTGGCTCCGTTATCGTTTTGGGAAACGCCGTTTGCCTCAGACCTGAACAGGTAGCAAAAGCAATCCAGGACCTCATTCCCATCCCTGGGGCCACTACAAACGATGCAAAAATGAAGGAGCTAGAAGACATGGAGAAACAGAAATCCCTAATAGACCAGAAAGCCGATTCCTTGGTGCGCAGGGAATTGTGGTTGGGGCTTGGCTACCTCGTTGTCCAAACAGCCGGGTTCATGAGGCTGACCTTTTGGGAACTCTCATGGGATGTGATGGAACCCATCTGTTTCTATGTGACTTCAATGTACTTCATGGCTTGCTATGCCTTCTTCCTCAGGACCTCCAAAGAGCCCTCTTTCGAAGGCTTCTTTCAGAGCCGTTTTGATGCCAAGCAGAAGAAATTGATGAAGGATCACAATTTCGATTTGGGTAGGTATGAGGAGCTTAGAAAGGCTTGTTTCCCCTTCTCTTCTTCTTCGTTGGAGTTAGGCAACCCATCAACTGCTTTTGATGGAACTAAGAGGATGCAAATGAAGTTTGGTTCCAATGTTCTATAA

Protein Analysis

360

Amino Acids

40.23

Weight (kDa)

9.14

Isoelectric Point (pI)

49.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MCU PF04678 167 - 323 1.4e-52 Mitochondrial calcium uniporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0011209)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23790 AT4G36820 AT5G66650
fragaria_vesca FvH4_1g14090
malus_domestica MD02G1155000.v1.1 MD15G1269400.v1.1 MD15G1269500.v1.1
prunus_persica Prupe.7G148300_v2.0.a1
pyrus_communis pycom02g12270 pycom15g23380
rosa_chinensis RchiOBHm_Chr2g0102751
rosa_laevigata RLG00000017177
rosa_multiflora Rmu_co8164376.1_g000001
rosa_roxburghii Rroxscaffold_2G00140340
rosa_rugosa Rorug02G0109400
rosa_samantha Rh2AG157700 Rh2BG164200 Rh2CG163900 Rh2DG163000
rosa_wichuraiana Rw2G012230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 707
AccB1I GGYRCC 1 cut(s) 304
AccI GTMKAC 1 cut(s) 332
AccII CGCG 1 cut(s) 197
AccIII TCCGGA 1 cut(s) 490
AciI CCGC 7 cut(s) 92, 110, 169, 299, 362, 404, 430
AclWI GGATC 4 cut(s) 482, 495, 501, 942
AcoI YGGCCR 2 cut(s) 104, 348
AcyI GRCGYC 2 cut(s) 192, 305
AfaI GTAC 1 cut(s) 830
AfiI CCNNNNNNNGG 8 cut(s) 109, 146, 165, 210, 273, 497, 614, 615
AgsI TTSAA 3 cut(s) 10, 37, 825
AjnI CCWGG 2 cut(s) 594, 613
AluBI AGCT 4 cut(s) 23, 247, 649, 967
AluI AGCT 4 cut(s) 23, 247, 649, 967
Alw26I GTCTC 4 cut(s) 144, 311, 362, 506
AlwI GGATC 4 cut(s) 482, 495, 501, 942
AlwNI CAGNNNCTG 1 cut(s) 72
Ama87I CYCGRG 4 cut(s) 120, 140, 203, 503
Aor13HI TCCGGA 1 cut(s) 490
AoxI GGCC 5 cut(s) 104, 186, 294, 348, 618
AspLEI GCGC 4 cut(s) 30, 307, 394, 708
AspS9I GGNCC 7 cut(s) 186, 217, 252, 295, 598, 618, 861
AsuC2I CCSGG 3 cut(s) 147, 267, 754
AsuHPI GGTGA 3 cut(s) 322, 418, 454
AsuII TTCGAA 1 cut(s) 882
AvaI CYCGRG 4 cut(s) 120, 140, 203, 503
AvaII GGWCC 4 cut(s) 217, 252, 598, 861
AxyI CCTNAGG 1 cut(s) 857
BamHI GGATCC 1 cut(s) 487
BanI GGYRCC 1 cut(s) 304
BanII GRGCYC 1 cut(s) 876
BbsI GAAGAC 1 cut(s) 660
BccI CCATC 7 cut(s) 232, 391, 617, 790, 812, 1027, 1028
BceAI ACGGC 3 cut(s) 375, 542, 885
BciT130I CCWGG 2 cut(s) 596, 615
BcnI CCSGG 3 cut(s) 147, 267, 754
BcoDI GTCTC 4 cut(s) 144, 311, 362, 506
BfaI CTAG 2 cut(s) 20, 650
BfoI RGCGCY 1 cut(s) 308
BglI GCCNNNNNGGC 1 cut(s) 302
BglII AGATCT 1 cut(s) 48
BisI GCNGC 1 cut(s) 111
BlpI GCTNAGC 1 cut(s) 24
BlsI GCNGC 1 cut(s) 112
Bme1390I CCNGG 5 cut(s) 147, 267, 596, 615, 754
Bme18I GGWCC 4 cut(s) 217, 252, 598, 861
BmeT110I CYCGRG 4 cut(s) 120, 140, 203, 503
BmgT120I GGNCC 7 cut(s) 186, 217, 252, 295, 598, 618, 861
BmiI GGNNCC 8 cut(s) 219, 297, 306, 489, 535, 619, 801, 1066
BmrFI CCNGG 5 cut(s) 147, 267, 596, 615, 754
BmsI GCATC 6 cut(s) 39, 106, 141, 622, 898, 1038
BpiI GAAGAC 1 cut(s) 660
BplI GAGNNNNNCTC 4 cut(s) 121, 153, 518, 550
Bpu10I CCTNAGC 1 cut(s) 248
Bpu1102I GCTNAGC 1 cut(s) 24
Bpu14I TTCGAA 1 cut(s) 882
BpuMI CCSGG 3 cut(s) 147, 267, 754
BsaBI GATNNNNATC 1 cut(s) 492
BsaHI GRCGYC 2 cut(s) 192, 305
BsaI GGTCTC 1 cut(s) 506
BsaJI CCNNGG 4 cut(s) 119, 613, 614, 699
BsaWI WCCGGW 1 cut(s) 490
BsaXI ACNNNNNCTCC 2 cut(s) 314, 344
Bsc4I CCNNNNNNNGG 8 cut(s) 109, 146, 165, 210, 273, 497, 614, 615
Bse118I RCCGGY 5 cut(s) 106, 188, 301, 307, 337
Bse21I CCTNAGG 1 cut(s) 857
Bse8I GATNNNNATC 1 cut(s) 492
BseAI TCCGGA 1 cut(s) 490
BseBI CCWGG 2 cut(s) 596, 615
BseDI CCNNGG 4 cut(s) 119, 613, 614, 699
BseGI GGATG 3 cut(s) 609, 796, 1053
BseJI GATNNNNATC 1 cut(s) 492
BseLI CCNNNNNNNGG 8 cut(s) 109, 146, 165, 210, 273, 497, 614, 615
BseMII CTCAG 5 cut(s) 15, 79, 262, 580, 871
BseRI GAGGAG 2 cut(s) 380, 977
BseX3I CGGCCG 1 cut(s) 104
Bsh1236I CGCG 1 cut(s) 197
Bsh1285I CGRYCG 2 cut(s) 107, 225
BshFI GGCC 5 cut(s) 106, 188, 296, 350, 620
BshNI GGYRCC 1 cut(s) 304
BsiEI CGRYCG 2 cut(s) 107, 225
BsiHKCI CYCGRG 4 cut(s) 120, 140, 203, 503
BslFI GGGAC 1 cut(s) 203
BslI CCNNNNNNNGG 8 cut(s) 109, 146, 165, 210, 273, 497, 614, 615
BsmAI GTCTC 4 cut(s) 144, 311, 362, 506
BsmBI CGTCTC 2 cut(s) 311, 362
BsmFI GGGAC 1 cut(s) 203
BsmI GAATGC 1 cut(s) 261
BsnI GGCC 5 cut(s) 106, 188, 296, 350, 620
Bso31I GGTCTC 1 cut(s) 506
BsoBI CYCGRG 4 cut(s) 120, 140, 203, 503
Bsp119I TTCGAA 1 cut(s) 882
Bsp1286I GDGCHC 1 cut(s) 876
Bsp13I TCCGGA 1 cut(s) 490
Bsp143I GATC 5 cut(s) 48, 222, 487, 493, 934
Bsp1720I GCTNAGC 1 cut(s) 24
Bsp68I TCGCGA 1 cut(s) 197
BspACI CCGC 7 cut(s) 92, 110, 169, 299, 362, 404, 430
BspANI GGCC 5 cut(s) 106, 188, 296, 350, 620
BspCNI CTCAG 5 cut(s) 16, 78, 261, 579, 870
BspEI TCCGGA 1 cut(s) 490
BspFNI CGCG 1 cut(s) 197
BspHI TCATGA 1 cut(s) 759
BspLI GGNNCC 8 cut(s) 219, 297, 306, 489, 535, 619, 801, 1066
BspPI GGATC 4 cut(s) 482, 495, 501, 942
BspT104I TTCGAA 1 cut(s) 882
BspT107I GGYRCC 1 cut(s) 304
BspTNI GGTCTC 1 cut(s) 506
BsrFI RCCGGY 5 cut(s) 106, 188, 301, 307, 337
BssAI RCCGGY 5 cut(s) 106, 188, 301, 307, 337
BssECI CCNNGG 4 cut(s) 119, 613, 614, 699
BssMI GATC 5 cut(s) 48, 222, 487, 493, 934
BssNI GRCGYC 2 cut(s) 192, 305
BssT1I CCWWGG 1 cut(s) 699
Bst2UI CCWGG 2 cut(s) 596, 615
Bst4CI ACNGT 1 cut(s) 290
Bst6I CTCTTC 1 cut(s) 997
BstACI GRCGYC 2 cut(s) 192, 305
BstBI TTCGAA 1 cut(s) 882
BstC8I GCNNGC 6 cut(s) 108, 130, 190, 303, 339, 841
BstDEI CTNAG 7 cut(s) 24, 65, 248, 566, 857, 968, 1041
BstF5I GGATG 3 cut(s) 609, 796, 1053
BstFNI CGCG 1 cut(s) 197
BstH2I RGCGCY 1 cut(s) 308
BstHHI GCGC 4 cut(s) 30, 307, 394, 708
BstKTI GATC 5 cut(s) 51, 225, 490, 496, 937
BstMAI GTCTC 4 cut(s) 144, 311, 362, 506
BstMBI GATC 5 cut(s) 48, 222, 487, 493, 934
BstMCI CGRYCG 2 cut(s) 107, 225
BstMWI GCNNNNNNNGC 7 cut(s) 103, 134, 194, 302, 347, 410, 845
BstNI CCWGG 2 cut(s) 596, 615
BstSCI CCNGG 5 cut(s) 145, 265, 594, 613, 752
BstUI CGCG 1 cut(s) 197
BstV2I GAAGAC 1 cut(s) 660
BstX2I RGATCY 3 cut(s) 48, 487, 493
BstYI RGATCY 3 cut(s) 48, 487, 493
BstZI CGGCCG 1 cut(s) 104
Bsu36I CCTNAGG 1 cut(s) 857
BsuRI GGCC 5 cut(s) 106, 188, 296, 350, 620
BtgZI GCGATG 1 cut(s) 118
BtsCI GGATG 3 cut(s) 609, 796, 1053
BtuMI TCGCGA 1 cut(s) 197
Cac8I GCNNGC 6 cut(s) 108, 130, 190, 303, 339, 841
CaiI CAGNNNCTG 1 cut(s) 72
CciI TCATGA 1 cut(s) 759
CfoI GCGC 4 cut(s) 30, 307, 394, 708
Cfr10I RCCGGY 5 cut(s) 106, 188, 301, 307, 337
Cfr13I GGNCC 7 cut(s) 186, 217, 252, 295, 598, 618, 861
CseI GACGC 1 cut(s) 181
Csp6I GTAC 1 cut(s) 829
CviAII CATG 5 cut(s) 346, 658, 760, 786, 835
CviQI GTAC 1 cut(s) 829
DdeI CTNAG 7 cut(s) 24, 65, 248, 566, 857, 968, 1041
DinI GGCGCC 1 cut(s) 306
DpnI GATC 5 cut(s) 50, 224, 489, 495, 936
DpnII GATC 5 cut(s) 48, 222, 487, 493, 934
EaeI YGGCCR 2 cut(s) 104, 348
EagI CGGCCG 1 cut(s) 104
Eam1104I CTCTTC 1 cut(s) 997
EarI CTCTTC 1 cut(s) 997
EciI GGCGGA 1 cut(s) 377
EclXI CGGCCG 1 cut(s) 104
Eco130I CCWWGG 1 cut(s) 699
Eco24I GRGCYC 1 cut(s) 876
Eco31I GGTCTC 1 cut(s) 506
Eco47I GGWCC 4 cut(s) 217, 252, 598, 861
Eco52I CGGCCG 1 cut(s) 104
Eco81I CCTNAGG 1 cut(s) 857
Eco88I CYCGRG 4 cut(s) 120, 140, 203, 503
EcoO109I RGGNCCY 2 cut(s) 598, 861
EcoRII CCWGG 2 cut(s) 594, 613
EcoT14I CCWWGG 1 cut(s) 699
EcoT38I GRGCYC 1 cut(s) 876
EgeI GGCGCC 1 cut(s) 306
EheI GGCGCC 1 cut(s) 306
ErhI CCWWGG 1 cut(s) 699
Esp3I CGTCTC 2 cut(s) 311, 362
FaeI CATG 5 cut(s) 349, 661, 763, 789, 838
FaqI GGGAC 1 cut(s) 203
FatI CATG 5 cut(s) 345, 657, 759, 785, 834
FauI CCCGC 1 cut(s) 306
FblI GTMKAC 1 cut(s) 332
Fnu4HI GCNGC 1 cut(s) 111
FokI GGATG 3 cut(s) 596, 803, 1060
FriOI GRGCYC 1 cut(s) 876
Fsp4HI GCNGC 1 cut(s) 111
FspBI CTAG 2 cut(s) 20, 650
FspI TGCGCA 1 cut(s) 707
GlaI GCGC 4 cut(s) 29, 306, 393, 707
GluI GCNGC 1 cut(s) 111
HaeII RGCGCY 1 cut(s) 308
HaeIII GGCC 5 cut(s) 106, 188, 296, 350, 620
HgaI GACGC 1 cut(s) 181
HhaI GCGC 4 cut(s) 30, 307, 394, 708
Hin1I GRCGYC 2 cut(s) 192, 305
Hin1II CATG 5 cut(s) 349, 661, 763, 789, 838
Hin6I GCGC 4 cut(s) 28, 305, 392, 706
HinP1I GCGC 4 cut(s) 28, 305, 392, 706
HinfI GANTC 2 cut(s) 277, 695
HphI GGTGA 3 cut(s) 322, 418, 454
Hpy166II GTNNAC 1 cut(s) 333
Hpy188I TCNGA 5 cut(s) 68, 454, 487, 569, 897
Hpy188III TCNNGA 9 cut(s) 10, 52, 122, 140, 196, 220, 491, 760, 859
Hpy8I GTNNAC 1 cut(s) 333
Hpy99I CGWCG 3 cut(s) 91, 197, 260
HpyAV CCTTC 7 cut(s) 319, 403, 637, 859, 878, 925, 997
HpyCH4III ACNGT 1 cut(s) 290
HpyCH4IV ACGT 2 cut(s) 437, 446
HpyCH4V TGCA 4 cut(s) 97, 471, 635, 1051
HpyF10VI GCNNNNNNNGC 7 cut(s) 103, 134, 194, 302, 347, 410, 845
HpyF3I CTNAG 7 cut(s) 24, 65, 248, 566, 857, 968, 1041
HpySE526I ACGT 2 cut(s) 437, 446
Hsp92I GRCGYC 2 cut(s) 192, 305
Hsp92II CATG 5 cut(s) 349, 661, 763, 789, 838
HspAI GCGC 4 cut(s) 28, 305, 392, 706
KasI GGCGCC 1 cut(s) 304
Kpn2I TCCGGA 1 cut(s) 490
KroI GCCGGC 4 cut(s) 106, 188, 301, 337
KroNI GCCGGC 4 cut(s) 108, 190, 303, 339
Kzo9I GATC 5 cut(s) 48, 222, 487, 493, 934
LmnI GCTCC 4 cut(s) 142, 539, 646, 964
LweI GCATC 6 cut(s) 39, 106, 141, 622, 898, 1038
MaeI CTAG 2 cut(s) 20, 650
MaeII ACGT 2 cut(s) 437, 446
MaeIII GTNAC 3 cut(s) 310, 442, 817
MalI GATC 5 cut(s) 50, 224, 489, 495, 936
MboI GATC 5 cut(s) 48, 222, 487, 493, 934
MboII GAAGA 7 cut(s) 25, 665, 844, 931, 984, 987, 990
MflI RGATCY 3 cut(s) 48, 487, 493
MhlI GDGCHC 1 cut(s) 876
MluCI AATT 3 cut(s) 713, 923, 940
Mly113I GGCGCC 1 cut(s) 305
MmeI TCCRAC 2 cut(s) 264, 984
MreI CGCCGGCG 1 cut(s) 301
MroI TCCGGA 1 cut(s) 490
MroNI GCCGGC 4 cut(s) 106, 188, 301, 337
MspR9I CCNGG 5 cut(s) 147, 267, 596, 615, 754
Mva1269I GAATGC 1 cut(s) 261
MvaI CCWGG 2 cut(s) 596, 615
MvnI CGCG 1 cut(s) 197
MwoI GCNNNNNNNGC 7 cut(s) 103, 134, 194, 302, 347, 410, 845
NaeI GCCGGC 4 cut(s) 108, 190, 303, 339
NarI GGCGCC 1 cut(s) 305
NciI CCSGG 3 cut(s) 147, 267, 754
NdeII GATC 5 cut(s) 48, 222, 487, 493, 934
NgoMIV GCCGGC 4 cut(s) 106, 188, 301, 337
NlaIII CATG 5 cut(s) 349, 661, 763, 789, 838
NlaIV GGNNCC 8 cut(s) 219, 297, 306, 489, 535, 619, 801, 1066
NmuCI GTSAC 3 cut(s) 310, 442, 817
NruI TCGCGA 1 cut(s) 197
NsbI TGCGCA 1 cut(s) 707
NspV TTCGAA 1 cut(s) 882
PagI TCATGA 1 cut(s) 759
PasI CCCWGGG 1 cut(s) 614
PcsI WCGNNNNNNNCGW 2 cut(s) 201, 443
PctI GAATGC 1 cut(s) 261
PdiI GCCGGC 4 cut(s) 108, 190, 303, 339
PfeI GAWTC 2 cut(s) 277, 695
PfoI TCCNGGA 2 cut(s) 265, 594
PkrI GCNGC 1 cut(s) 112
Ple19I CGATCG 1 cut(s) 225
PluTI GGCGCC 1 cut(s) 308
PpuMI RGGWCCY 2 cut(s) 598, 861
Psp5II RGGWCCY 2 cut(s) 598, 861
Psp6I CCWGG 2 cut(s) 594, 613
PspGI CCWGG 2 cut(s) 594, 613
PspN4I GGNNCC 8 cut(s) 219, 297, 306, 489, 535, 619, 801, 1066
PspPI GGNCC 7 cut(s) 186, 217, 252, 295, 598, 618, 861
PspPPI RGGWCCY 2 cut(s) 598, 861
PstNI CAGNNNCTG 1 cut(s) 72
PsuI RGATCY 3 cut(s) 48, 487, 493
PvuI CGATCG 1 cut(s) 225
RruI TCGCGA 1 cut(s) 197
RsaI GTAC 1 cut(s) 830
RsaNI GTAC 1 cut(s) 829
SatI GCNGC 1 cut(s) 111
Sau3AI GATC 5 cut(s) 48, 222, 487, 493, 934
Sau96I GGNCC 7 cut(s) 186, 217, 252, 295, 598, 618, 861
ScrFI CCNGG 5 cut(s) 147, 267, 596, 615, 754
SduI GDGCHC 1 cut(s) 876
SfaNI GCATC 6 cut(s) 39, 106, 141, 622, 898, 1038
SfoI GGCGCC 1 cut(s) 306
SfuI TTCGAA 1 cut(s) 882
SgrAI CRCCGGYG 2 cut(s) 301, 307
SinI GGWCC 4 cut(s) 217, 252, 598, 861
Sse9I AATT 3 cut(s) 713, 923, 940
SsiI CCGC 7 cut(s) 92, 110, 169, 299, 362, 404, 430
SspDI GGCGCC 1 cut(s) 304
SspMI CTAG 2 cut(s) 20, 650
StyD4I CCNGG 5 cut(s) 145, 265, 594, 613, 752
StyI CCWWGG 1 cut(s) 699
TaaI ACNGT 1 cut(s) 290
TaiI ACGT 2 cut(s) 440, 449
TaqI TCGA 3 cut(s) 53, 882, 945
TaqII GACCGA 1 cut(s) 205
TasI AATT 3 cut(s) 713, 923, 940
TatI WGTACW 1 cut(s) 828
TauI GCSGC 1 cut(s) 113
TfiI GAWTC 2 cut(s) 277, 695
TseFI GTSAC 3 cut(s) 310, 442, 817
Tsp45I GTSAC 3 cut(s) 310, 442, 817
TspDTI ATGAA 6 cut(s) 163, 656, 748, 823, 944, 1070
TspGWI ACGGA 6 cut(s) 244, 329, 335, 410, 488, 526
VpaK11BI GGWCC 4 cut(s) 217, 252, 598, 861
XmiI GTMKAC 1 cut(s) 332
XspI CTAG 2 cut(s) 20, 650
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.