Rh2BG164200

Mitochondrial calcium uniporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0011209
Physical Location & Seq
Forward (+)
14416895 .. 14419597
2703 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG164200.1

Sequence Viewer

Length: 1086 bp
ATGGCGTTCAAGAAAACCCTAGCTGAGCGCATCTTCAATATCTCCAAGATCTCGACCCAAACCCTCAGAAACTGCCGGATTTCCTCGTCGGCGGTGCATCTCCGGCCGGCGGCGAAGCCTCGGGAGGCGAGCATCGCTCCCGAGCCGGGAGACAATGCCATTTTCCGGCGGTTCATTCACAGGAGGGCCGGCGTTGCGAAGCCGGAGATCTGGTCGGTTCCGATGGGCGAGAACCTGATGGAAAAGCTCAGGTCCGTCGGCATTCCCGGAAGCCGGATTCGGTTGGACGGTTTGGCTCCGCCGGCGCCGGTGCCGGTGCCGGAGAAGGAGAAGGTGTCTGCGCCGGCTGTCATGGCCGGACTGACGGCGGAGGAGACGAGGAAGTTGTTGAGGGTGGCGCAGATGGAGGCGGTGAAGGCGAAACTCCGGGAGGTGAGGAAAACGTGGGTGACGTATTCGGAGTTTGTTGGGATTTGCACGGAGGGTTGTTCGGATCCGGATCTGGGTCTCGGGTTTGCGAAATCGCTGGACGAGAGTGGCTCCGTTATCGTTTTGGGAAACGCCGTTTGCCTCAGACCCGAACAGGTAGCAAAAGCAATCCAGGACCTCATTCCCATCCCTGGGGCCACTCCAAACGATGCAAAAATGAAGGAGCTAGAAGACATGGAGAAACAGAAATCCCTAATAGACCAGAAAGCCGATTCCTTGGTGCGCAGGGAACTGTGGTTGGGGCTTGGCTATCTCGTTGTCCAAACAGCCGGGTTCATGAGGCTGACCTTTTGGGAACTCTCATGGGATGTGATGGAACCCATCTGTTTCTATGTGACTTCAATGTACTTCATGGCTTGCTATGCCTTCTTCCTCAGGACCTCCAAAGAGCCCTCTTTCGAAGGCTTCTTCCAGAGCCGTTTTGATGCCAAGCAGAAGAAATTGATGAAGGATCACAATTTCGATTTGGGTAGGTATGAGGAGCTTAGAAAAGCTTGTTACCCCTTCTCTTCTTCTTCGTCGGAGTTAGGCAACCCATCAACTGCTTTTGATGGAACTAAGAGGATGCAAATGAAGTTTGGTTCCAATGTTCTATAA

Protein Analysis

361

Amino Acids

40.42

Weight (kDa)

9.2

Isoelectric Point (pI)

51.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MCU PF04678 169 - 325 2.3e-52 Mitochondrial calcium uniporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011209)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23790 AT4G36820 AT5G66650
fragaria_vesca FvH4_1g14090
malus_domestica MD02G1155000.v1.1 MD15G1269400.v1.1 MD15G1269500.v1.1
prunus_persica Prupe.7G148300_v2.0.a1
pyrus_communis pycom02g12270 pycom15g23380
rosa_chinensis RchiOBHm_Chr2g0102751
rosa_laevigata RLG00000017177
rosa_multiflora Rmu_co8164376.1_g000001
rosa_roxburghii Rroxscaffold_2G00140340
rosa_rugosa Rorug02G0109400
rosa_samantha Rh2AG157700 Rh2BG164200 Rh2CG163900 Rh2DG163000
rosa_wichuraiana Rw2G012230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 713
AccB1I GGYRCC 3 cut(s) 304, 310, 316
AccIII TCCGGA 1 cut(s) 496
AciI CCGC 6 cut(s) 92, 110, 169, 299, 368, 410
AclWI GGATC 4 cut(s) 488, 501, 507, 948
AcoI YGGCCR 2 cut(s) 104, 354
AcyI GRCGYC 1 cut(s) 305
AfaI GTAC 1 cut(s) 836
AfiI CCNNNNNNNGG 7 cut(s) 109, 146, 165, 273, 503, 620, 621
AgsI TTSAA 3 cut(s) 10, 37, 831
AjnI CCWGG 2 cut(s) 600, 619
AluBI AGCT 5 cut(s) 23, 247, 655, 973, 983
AluI AGCT 5 cut(s) 23, 247, 655, 973, 983
Alw26I GTCTC 3 cut(s) 144, 368, 512
AlwI GGATC 4 cut(s) 488, 501, 507, 948
AlwNI CAGNNNCTG 1 cut(s) 72
Ama87I CYCGRG 3 cut(s) 120, 140, 509
Aor13HI TCCGGA 1 cut(s) 496
AoxI GGCC 4 cut(s) 104, 186, 354, 624
AspLEI GCGC 5 cut(s) 30, 307, 343, 400, 714
AspS9I GGNCC 5 cut(s) 186, 252, 604, 624, 867
AsuC2I CCSGG 4 cut(s) 147, 267, 428, 760
AsuHPI GGTGA 3 cut(s) 424, 445, 460
AsuII TTCGAA 1 cut(s) 888
AvaI CYCGRG 3 cut(s) 120, 140, 509
AvaII GGWCC 3 cut(s) 252, 604, 867
AxyI CCTNAGG 1 cut(s) 863
BamHI GGATCC 1 cut(s) 493
BanI GGYRCC 3 cut(s) 304, 310, 316
BanII GRGCYC 1 cut(s) 882
BbsI GAAGAC 1 cut(s) 666
BccI CCATC 8 cut(s) 217, 232, 397, 623, 796, 818, 1033, 1034
BceAI ACGGC 3 cut(s) 381, 548, 891
BciT130I CCWGG 2 cut(s) 602, 621
BcnI CCSGG 4 cut(s) 147, 267, 428, 760
BcoDI GTCTC 3 cut(s) 144, 368, 512
BfaI CTAG 2 cut(s) 20, 656
BfoI RGCGCY 1 cut(s) 308
BglII AGATCT 2 cut(s) 48, 207
BisI GCNGC 1 cut(s) 111
BlpI GCTNAGC 1 cut(s) 24
BlsI GCNGC 1 cut(s) 112
Bme1390I CCNGG 6 cut(s) 147, 267, 428, 602, 621, 760
Bme18I GGWCC 3 cut(s) 252, 604, 867
BmeT110I CYCGRG 3 cut(s) 120, 140, 509
BmgT120I GGNCC 5 cut(s) 186, 252, 604, 624, 867
BmrFI CCNGG 6 cut(s) 147, 267, 428, 602, 621, 760
BmsI GCATC 6 cut(s) 39, 106, 141, 628, 904, 1044
BpiI GAAGAC 1 cut(s) 666
BplI GAGNNNNNCTC 4 cut(s) 121, 153, 524, 556
Bpu10I CCTNAGC 1 cut(s) 248
Bpu1102I GCTNAGC 1 cut(s) 24
Bpu14I TTCGAA 1 cut(s) 888
BpuMI CCSGG 4 cut(s) 147, 267, 428, 760
BsaBI GATNNNNATC 1 cut(s) 498
BsaHI GRCGYC 1 cut(s) 305
BsaI GGTCTC 1 cut(s) 512
BsaJI CCNNGG 4 cut(s) 119, 619, 620, 705
BsaWI WCCGGW 1 cut(s) 496
BsaXI ACNNNNNCTCC 4 cut(s) 197, 227, 320, 350
Bsc4I CCNNNNNNNGG 7 cut(s) 109, 146, 165, 273, 503, 620, 621
Bse118I RCCGGY 6 cut(s) 106, 188, 301, 307, 313, 343
Bse21I CCTNAGG 1 cut(s) 863
Bse8I GATNNNNATC 1 cut(s) 498
BseAI TCCGGA 1 cut(s) 496
BseBI CCWGG 2 cut(s) 602, 621
BseDI CCNNGG 4 cut(s) 119, 619, 620, 705
BseGI GGATG 3 cut(s) 615, 802, 1059
BseJI GATNNNNATC 1 cut(s) 498
BseLI CCNNNNNNNGG 7 cut(s) 109, 146, 165, 273, 503, 620, 621
BseMII CTCAG 5 cut(s) 15, 79, 262, 586, 877
BseRI GAGGAG 2 cut(s) 386, 983
BseX3I CGGCCG 1 cut(s) 104
Bsh1285I CGRYCG 1 cut(s) 107
BshFI GGCC 4 cut(s) 106, 188, 356, 626
BshNI GGYRCC 3 cut(s) 304, 310, 316
BsiEI CGRYCG 1 cut(s) 107
BsiHKCI CYCGRG 3 cut(s) 120, 140, 509
BslI CCNNNNNNNGG 7 cut(s) 109, 146, 165, 273, 503, 620, 621
BsmAI GTCTC 3 cut(s) 144, 368, 512
BsmBI CGTCTC 1 cut(s) 368
BsmI GAATGC 1 cut(s) 261
BsnI GGCC 4 cut(s) 106, 188, 356, 626
Bso31I GGTCTC 1 cut(s) 512
BsoBI CYCGRG 3 cut(s) 120, 140, 509
Bsp119I TTCGAA 1 cut(s) 888
Bsp1286I GDGCHC 1 cut(s) 882
Bsp13I TCCGGA 1 cut(s) 496
Bsp143I GATC 5 cut(s) 48, 207, 493, 499, 940
Bsp1720I GCTNAGC 1 cut(s) 24
BspACI CCGC 6 cut(s) 92, 110, 169, 299, 368, 410
BspANI GGCC 4 cut(s) 106, 188, 356, 626
BspCNI CTCAG 5 cut(s) 16, 78, 261, 585, 876
BspEI TCCGGA 1 cut(s) 496
BspHI TCATGA 1 cut(s) 765
BspPI GGATC 4 cut(s) 488, 501, 507, 948
BspT104I TTCGAA 1 cut(s) 888
BspT107I GGYRCC 3 cut(s) 304, 310, 316
BspTNI GGTCTC 1 cut(s) 512
BsrFI RCCGGY 6 cut(s) 106, 188, 301, 307, 313, 343
BssAI RCCGGY 6 cut(s) 106, 188, 301, 307, 313, 343
BssECI CCNNGG 4 cut(s) 119, 619, 620, 705
BssMI GATC 5 cut(s) 48, 207, 493, 499, 940
BssNI GRCGYC 1 cut(s) 305
BssT1I CCWWGG 1 cut(s) 705
Bst2UI CCWGG 2 cut(s) 602, 621
Bst4CI ACNGT 2 cut(s) 290, 723
Bst6I CTCTTC 1 cut(s) 1003
BstACI GRCGYC 1 cut(s) 305
BstBI TTCGAA 1 cut(s) 888
BstC8I GCNNGC 6 cut(s) 108, 130, 190, 303, 345, 847
BstDEI CTNAG 7 cut(s) 24, 65, 248, 572, 863, 974, 1047
BstF5I GGATG 3 cut(s) 615, 802, 1059
BstH2I RGCGCY 1 cut(s) 308
BstHHI GCGC 5 cut(s) 30, 307, 343, 400, 714
BstKTI GATC 5 cut(s) 51, 210, 496, 502, 943
BstMAI GTCTC 3 cut(s) 144, 368, 512
BstMBI GATC 5 cut(s) 48, 207, 493, 499, 940
BstMCI CGRYCG 1 cut(s) 107
BstMWI GCNNNNNNNGC 7 cut(s) 103, 134, 194, 302, 353, 416, 851
BstNI CCWGG 2 cut(s) 602, 621
BstSCI CCNGG 6 cut(s) 145, 265, 426, 600, 619, 758
BstV2I GAAGAC 1 cut(s) 666
BstX2I RGATCY 4 cut(s) 48, 207, 493, 499
BstYI RGATCY 4 cut(s) 48, 207, 493, 499
BstZI CGGCCG 1 cut(s) 104
Bsu36I CCTNAGG 1 cut(s) 863
BsuRI GGCC 4 cut(s) 106, 188, 356, 626
BtgZI GCGATG 1 cut(s) 118
BtsCI GGATG 3 cut(s) 615, 802, 1059
Cac8I GCNNGC 6 cut(s) 108, 130, 190, 303, 345, 847
CaiI CAGNNNCTG 1 cut(s) 72
CciI TCATGA 1 cut(s) 765
CfoI GCGC 5 cut(s) 30, 307, 343, 400, 714
Cfr10I RCCGGY 6 cut(s) 106, 188, 301, 307, 313, 343
Cfr13I GGNCC 5 cut(s) 186, 252, 604, 624, 867
Csp6I GTAC 1 cut(s) 835
CviAII CATG 5 cut(s) 352, 664, 766, 792, 841
CviQI GTAC 1 cut(s) 835
DdeI CTNAG 7 cut(s) 24, 65, 248, 572, 863, 974, 1047
DinI GGCGCC 1 cut(s) 306
DpnI GATC 5 cut(s) 50, 209, 495, 501, 942
DpnII GATC 5 cut(s) 48, 207, 493, 499, 940
EaeI YGGCCR 2 cut(s) 104, 354
EagI CGGCCG 1 cut(s) 104
Eam1104I CTCTTC 1 cut(s) 1003
EarI CTCTTC 1 cut(s) 1003
EciI GGCGGA 2 cut(s) 288, 383
EclXI CGGCCG 1 cut(s) 104
Eco130I CCWWGG 1 cut(s) 705
Eco24I GRGCYC 1 cut(s) 882
Eco31I GGTCTC 1 cut(s) 512
Eco47I GGWCC 3 cut(s) 252, 604, 867
Eco52I CGGCCG 1 cut(s) 104
Eco81I CCTNAGG 1 cut(s) 863
Eco88I CYCGRG 3 cut(s) 120, 140, 509
EcoO109I RGGNCCY 2 cut(s) 604, 867
EcoRII CCWGG 2 cut(s) 600, 619
EcoT14I CCWWGG 1 cut(s) 705
EcoT38I GRGCYC 1 cut(s) 882
EgeI GGCGCC 1 cut(s) 306
EheI GGCGCC 1 cut(s) 306
ErhI CCWWGG 1 cut(s) 705
Esp3I CGTCTC 1 cut(s) 368
FaeI CATG 5 cut(s) 355, 667, 769, 795, 844
FaiI YATR 9 cut(s) 353, 665, 767, 793, 822, 842, 852, 966, 1084
FatI CATG 5 cut(s) 351, 663, 765, 791, 840
Fnu4HI GCNGC 1 cut(s) 111
FokI GGATG 3 cut(s) 602, 809, 1066
FriOI GRGCYC 1 cut(s) 882
Fsp4HI GCNGC 1 cut(s) 111
FspBI CTAG 2 cut(s) 20, 656
FspI TGCGCA 1 cut(s) 713
GlaI GCGC 5 cut(s) 29, 306, 342, 399, 713
GluI GCNGC 1 cut(s) 111
HaeII RGCGCY 1 cut(s) 308
HaeIII GGCC 4 cut(s) 106, 188, 356, 626
HhaI GCGC 5 cut(s) 30, 307, 343, 400, 714
Hin1I GRCGYC 1 cut(s) 305
Hin1II CATG 5 cut(s) 355, 667, 769, 795, 844
Hin6I GCGC 5 cut(s) 28, 305, 341, 398, 712
HinP1I GCGC 5 cut(s) 28, 305, 341, 398, 712
HindIII AAGCTT 1 cut(s) 981
HinfI GANTC 2 cut(s) 277, 701
HphI GGTGA 3 cut(s) 424, 445, 460
Hpy188I TCNGA 6 cut(s) 68, 222, 460, 493, 575, 1012
Hpy188III TCNNGA 8 cut(s) 10, 52, 122, 140, 497, 766, 865, 901
Hpy99I CGWCG 3 cut(s) 91, 260, 1012
HpyAV CCTTC 8 cut(s) 319, 325, 409, 643, 865, 884, 931, 1003
HpyCH4III ACNGT 2 cut(s) 290, 723
HpyCH4IV ACGT 2 cut(s) 443, 452
HpyCH4V TGCA 4 cut(s) 97, 477, 641, 1057
HpyF10VI GCNNNNNNNGC 7 cut(s) 103, 134, 194, 302, 353, 416, 851
HpyF3I CTNAG 7 cut(s) 24, 65, 248, 572, 863, 974, 1047
HpySE526I ACGT 2 cut(s) 443, 452
Hsp92I GRCGYC 1 cut(s) 305
Hsp92II CATG 5 cut(s) 355, 667, 769, 795, 844
HspAI GCGC 5 cut(s) 28, 305, 341, 398, 712
KasI GGCGCC 1 cut(s) 304
Kpn2I TCCGGA 1 cut(s) 496
KroI GCCGGC 4 cut(s) 106, 188, 301, 343
KroNI GCCGGC 4 cut(s) 108, 190, 303, 345
Kzo9I GATC 5 cut(s) 48, 207, 493, 499, 940
LmnI GCTCC 5 cut(s) 142, 301, 545, 652, 970
LweI GCATC 6 cut(s) 39, 106, 141, 628, 904, 1044
MaeI CTAG 2 cut(s) 20, 656
MaeII ACGT 2 cut(s) 443, 452
MaeIII GTNAC 3 cut(s) 448, 823, 986
MalI GATC 5 cut(s) 50, 209, 495, 501, 942
MboI GATC 5 cut(s) 48, 207, 493, 499, 940
MboII GAAGA 8 cut(s) 25, 671, 850, 889, 937, 990, 993, 996
MflI RGATCY 4 cut(s) 48, 207, 493, 499
MhlI GDGCHC 1 cut(s) 882
MluCI AATT 2 cut(s) 929, 946
Mly113I GGCGCC 1 cut(s) 305
MmeI TCCRAC 2 cut(s) 264, 990
MreI CGCCGGCG 1 cut(s) 301
MroI TCCGGA 1 cut(s) 496
MroNI GCCGGC 4 cut(s) 106, 188, 301, 343
MspR9I CCNGG 6 cut(s) 147, 267, 428, 602, 621, 760
Mva1269I GAATGC 1 cut(s) 261
MvaI CCWGG 2 cut(s) 602, 621
MwoI GCNNNNNNNGC 7 cut(s) 103, 134, 194, 302, 353, 416, 851
NaeI GCCGGC 4 cut(s) 108, 190, 303, 345
NarI GGCGCC 1 cut(s) 305
NciI CCSGG 4 cut(s) 147, 267, 428, 760
NdeII GATC 5 cut(s) 48, 207, 493, 499, 940
NgoMIV GCCGGC 4 cut(s) 106, 188, 301, 343
NlaIII CATG 5 cut(s) 355, 667, 769, 795, 844
NmuCI GTSAC 2 cut(s) 448, 823
NsbI TGCGCA 1 cut(s) 713
NspV TTCGAA 1 cut(s) 888
PagI TCATGA 1 cut(s) 765
PasI CCCWGGG 1 cut(s) 620
PcsI WCGNNNNNNNCGW 1 cut(s) 449
PctI GAATGC 1 cut(s) 261
PdiI GCCGGC 4 cut(s) 108, 190, 303, 345
PfeI GAWTC 2 cut(s) 277, 701
PfoI TCCNGGA 3 cut(s) 265, 426, 600
PkrI GCNGC 1 cut(s) 112
PluTI GGCGCC 1 cut(s) 308
PpuMI RGGWCCY 2 cut(s) 604, 867
Psp5II RGGWCCY 2 cut(s) 604, 867
Psp6I CCWGG 2 cut(s) 600, 619
PspGI CCWGG 2 cut(s) 600, 619
PspPI GGNCC 5 cut(s) 186, 252, 604, 624, 867
PspPPI RGGWCCY 2 cut(s) 604, 867
PstNI CAGNNNCTG 1 cut(s) 72
PsuI RGATCY 4 cut(s) 48, 207, 493, 499
RsaI GTAC 1 cut(s) 836
RsaNI GTAC 1 cut(s) 835
SatI GCNGC 1 cut(s) 111
Sau3AI GATC 5 cut(s) 48, 207, 493, 499, 940
Sau96I GGNCC 5 cut(s) 186, 252, 604, 624, 867
ScrFI CCNGG 6 cut(s) 147, 267, 428, 602, 621, 760
SduI GDGCHC 1 cut(s) 882
SfaNI GCATC 6 cut(s) 39, 106, 141, 628, 904, 1044
SfoI GGCGCC 1 cut(s) 306
SfuI TTCGAA 1 cut(s) 888
SgrAI CRCCGGYG 2 cut(s) 301, 307
SinI GGWCC 3 cut(s) 252, 604, 867
Sse9I AATT 2 cut(s) 929, 946
SsiI CCGC 6 cut(s) 92, 110, 169, 299, 368, 410
SspDI GGCGCC 1 cut(s) 304
SspMI CTAG 2 cut(s) 20, 656
StyD4I CCNGG 6 cut(s) 145, 265, 426, 600, 619, 758
StyI CCWWGG 1 cut(s) 705
TaaI ACNGT 2 cut(s) 290, 723
TaiI ACGT 2 cut(s) 446, 455
TaqI TCGA 3 cut(s) 53, 888, 951
TasI AATT 2 cut(s) 929, 946
TatI WGTACW 1 cut(s) 834
TauI GCSGC 1 cut(s) 113
TfiI GAWTC 2 cut(s) 277, 701
TseFI GTSAC 2 cut(s) 448, 823
Tsp45I GTSAC 2 cut(s) 448, 823
TspDTI ATGAA 6 cut(s) 163, 662, 754, 829, 950, 1076
TspGWI ACGGA 3 cut(s) 244, 494, 532
VpaK11BI GGWCC 3 cut(s) 252, 604, 867
XspI CTAG 2 cut(s) 20, 656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.