AT5G66930

Autophagy-related protein 101

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007886
Physical Location & Seq
Forward (+)
26725839 .. 26727758
1920 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G66930.2

Sequence Viewer

Length: 474 bp
ATGAATTGCGAAGTTTGTCAGCTAAAGGAACTGGAAGTGGAATCCTTCGAGATACGCGAAGTTTTGCGCTGCATTCTACATACGATTGTCTTTCATCGTGCGCTTGGTCTTATCCGCCCTAAAGATATTGATTTGGAGCTTTTTGAGATCACTTATGTACAATGCGGTGAAATTGAAGTGGAGAAGAAAATCGATGAAAAGATTGAGCAGTTCATTAACTGGATTGAGAAACACCCAAACAAAAAGAGTCAGATTTGTCTATCGTTCTATGAAGTCAAAAGCAAACAGCCTTCTTGGTTCACCAAAATCGAACGGTTGTACTGGGAACAATGGTACATCAATCTGAATGTGCTTCAGCCCACTAAGCCCCCTGTCGGAAAGTCTCACCATTCTAAACTAGTTATGGACCCTGGAGGTTCGATTGATCAATTTCCTGTTCTCATCTCCAAAAATTACATCTTTTTATTATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04136 ko04140 ko04211 map04136 map04140 map04211
Pfam Domains
Protein Families
ATG101

Protein Analysis

157

Amino Acids

18.66

Weight (kDa)

6.22

Isoelectric Point (pI)

41.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG101 PF07855 10 - 177 1.4e-52 Autophagy-related protein 101
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 472
AccII CGCG 1 cut(s) 57
AciI CCGC 2 cut(s) 115, 165
AcuI CTGAAG 1 cut(s) 338
AfaI GTAC 3 cut(s) 159, 320, 335
AfiI CCNNNNNNNGG 1 cut(s) 374
AgsI TTSAA 1 cut(s) 176
AhlI ACTAGT 1 cut(s) 397
AjnI CCWGG 1 cut(s) 409
AluBI AGCT 2 cut(s) 22, 139
AluI AGCT 2 cut(s) 22, 139
Alw26I GTCTC 1 cut(s) 387
ApeKI GCWGC 1 cut(s) 69
AspLEI GCGC 2 cut(s) 69, 103
AspS9I GGNCC 1 cut(s) 406
AsuHPI GGTGA 3 cut(s) 179, 292, 377
AvaII GGWCC 1 cut(s) 406
BbvI GCAGC 1 cut(s) 56
BciT130I CCWGG 1 cut(s) 411
BclI TGATCA 1 cut(s) 424
BcoDI GTCTC 1 cut(s) 387
BcuI ACTAGT 1 cut(s) 397
BfaI CTAG 1 cut(s) 398
BisI GCNGC 1 cut(s) 70
BlsI GCNGC 1 cut(s) 71
Bme1390I CCNGG 1 cut(s) 411
Bme18I GGWCC 1 cut(s) 406
BmgT120I GGNCC 1 cut(s) 406
BmiI GGNNCC 1 cut(s) 408
BmrFI CCNGG 1 cut(s) 411
BmrI ACTGGG 1 cut(s) 331
BmuI ACTGGG 1 cut(s) 331
BpmI CTGGAG 1 cut(s) 432
Bsa29I ATCGAT 1 cut(s) 192
BsaJI CCNNGG 1 cut(s) 409
Bsc4I CCNNNNNNNGG 1 cut(s) 374
Bse1I ACTGG 3 cut(s) 36, 224, 326
BseBI CCWGG 1 cut(s) 411
BseCI ATCGAT 1 cut(s) 192
BseDI CCNNGG 1 cut(s) 409
BseLI CCNNNNNNNGG 1 cut(s) 374
BseNI ACTGG 3 cut(s) 36, 224, 326
BseXI GCAGC 1 cut(s) 56
Bsh1236I CGCG 1 cut(s) 57
BshVI ATCGAT 1 cut(s) 192
BslI CCNNNNNNNGG 1 cut(s) 374
BsmAI GTCTC 1 cut(s) 387
BsmI GAATGC 1 cut(s) 72
Bsp1407I TGTACA 1 cut(s) 157
Bsp143I GATC 2 cut(s) 147, 424
BspACI CCGC 2 cut(s) 115, 165
BspDI ATCGAT 1 cut(s) 192
BspFNI CGCG 1 cut(s) 57
BspLI GGNNCC 1 cut(s) 408
BsrGI TGTACA 1 cut(s) 157
BsrI ACTGG 3 cut(s) 36, 224, 326
BssECI CCNNGG 1 cut(s) 409
BssMI GATC 2 cut(s) 147, 424
Bst2UI CCWGG 1 cut(s) 411
Bst4CI ACNGT 1 cut(s) 315
BstAUI TGTACA 1 cut(s) 157
BstDEI CTNAG 1 cut(s) 363
BstFNI CGCG 1 cut(s) 57
BstHHI GCGC 2 cut(s) 69, 103
BstKTI GATC 2 cut(s) 150, 427
BstMAI GTCTC 1 cut(s) 387
BstMBI GATC 2 cut(s) 147, 424
BstMWI GCNNNNNNNGC 1 cut(s) 364
BstNI CCWGG 1 cut(s) 411
BstSCI CCNGG 1 cut(s) 409
BstUI CGCG 1 cut(s) 57
BstV1I GCAGC 1 cut(s) 56
Bsu15I ATCGAT 1 cut(s) 192
BsuTUI ATCGAT 1 cut(s) 192
CfoI GCGC 2 cut(s) 69, 103
Cfr13I GGNCC 1 cut(s) 406
ClaI ATCGAT 1 cut(s) 192
Csp6I GTAC 3 cut(s) 158, 319, 334
CviJI RGCY 5 cut(s) 22, 139, 289, 358, 367
CviKI_1 RGCY 5 cut(s) 22, 139, 289, 358, 367
CviQI GTAC 3 cut(s) 158, 319, 334
DdeI CTNAG 1 cut(s) 363
DpnI GATC 2 cut(s) 149, 426
DpnII GATC 2 cut(s) 147, 424
EciI GGCGGA 1 cut(s) 104
Eco47I GGWCC 1 cut(s) 406
Eco57I CTGAAG 1 cut(s) 338
EcoRII CCWGG 1 cut(s) 409
FaiI YATR 5 cut(s) 81, 156, 270, 404, 472
FbaI TGATCA 1 cut(s) 424
Fnu4HI GCNGC 1 cut(s) 70
Fsp4HI GCNGC 1 cut(s) 70
FspBI CTAG 1 cut(s) 398
GlaI GCGC 2 cut(s) 68, 102
GluI GCNGC 1 cut(s) 70
GsuI CTGGAG 1 cut(s) 432
HhaI GCGC 2 cut(s) 69, 103
Hin6I GCGC 2 cut(s) 67, 101
HinP1I GCGC 2 cut(s) 67, 101
HinfI GANTC 2 cut(s) 41, 247
HphI GGTGA 3 cut(s) 179, 292, 377
Hpy166II GTNNAC 1 cut(s) 300
Hpy188I TCNGA 3 cut(s) 252, 345, 377
Hpy188III TCNNGA 1 cut(s) 49
Hpy8I GTNNAC 1 cut(s) 300
HpyAV CCTTC 2 cut(s) 55, 300
HpyCH4III ACNGT 1 cut(s) 315
HpyCH4V TGCA 1 cut(s) 72
HpyF10VI GCNNNNNNNGC 1 cut(s) 364
HpyF3I CTNAG 1 cut(s) 363
HspAI GCGC 2 cut(s) 67, 101
Ksp22I TGATCA 1 cut(s) 424
Kzo9I GATC 2 cut(s) 147, 424
LmnI GCTCC 1 cut(s) 136
LpnPI CCDG 7 cut(s) 17, 205, 307, 384, 396, 423, 447
Lsp1109I GCAGC 1 cut(s) 56
MaeI CTAG 1 cut(s) 398
MalI GATC 2 cut(s) 149, 426
MboI GATC 2 cut(s) 147, 424
MboII GAAGA 1 cut(s) 196
MluCI AATT 4 cut(s) 4, 171, 428, 451
MlyI GAGTC 1 cut(s) 256
MmeI TCCRAC 1 cut(s) 355
MnlI CCTC 1 cut(s) 407
MseI TTAA 1 cut(s) 216
MspR9I CCNGG 1 cut(s) 411
Mva1269I GAATGC 1 cut(s) 72
MvaI CCWGG 1 cut(s) 411
MvnI CGCG 1 cut(s) 57
MwoI GCNNNNNNNGC 1 cut(s) 364
NdeII GATC 2 cut(s) 147, 424
NlaIV GGNNCC 1 cut(s) 408
PcsI WCGNNNNNNNCGW 1 cut(s) 54
PctI GAATGC 1 cut(s) 72
PfeI GAWTC 1 cut(s) 41
PkrI GCNGC 1 cut(s) 71
PleI GAGTC 1 cut(s) 255
PpsI GAGTC 1 cut(s) 255
PsiI TTATAA 1 cut(s) 472
Psp6I CCWGG 1 cut(s) 409
PspGI CCWGG 1 cut(s) 409
PspN4I GGNNCC 1 cut(s) 408
PspPI GGNCC 1 cut(s) 406
RsaI GTAC 3 cut(s) 159, 320, 335
RsaNI GTAC 3 cut(s) 158, 319, 334
SaqAI TTAA 1 cut(s) 216
SatI GCNGC 1 cut(s) 70
Sau3AI GATC 2 cut(s) 147, 424
Sau96I GGNCC 1 cut(s) 406
SchI GAGTC 1 cut(s) 256
ScrFI CCNGG 1 cut(s) 411
SetI ASST 3 cut(s) 24, 141, 418
SinI GGWCC 1 cut(s) 406
SpeI ACTAGT 1 cut(s) 397
Sse9I AATT 4 cut(s) 4, 171, 428, 451
SsiI CCGC 2 cut(s) 115, 165
SspMI CTAG 1 cut(s) 398
StyD4I CCNGG 1 cut(s) 409
TaaI ACNGT 1 cut(s) 315
TaqI TCGA 4 cut(s) 48, 192, 309, 419
TasI AATT 4 cut(s) 4, 171, 428, 451
TatI WGTACW 2 cut(s) 157, 318
TfiI GAWTC 1 cut(s) 41
Tru1I TTAA 1 cut(s) 216
Tru9I TTAA 1 cut(s) 216
TseI GCWGC 1 cut(s) 69
TspDTI ATGAA 5 cut(s) 17, 83, 202, 210, 285
VpaK11BI GGWCC 1 cut(s) 406
XspI CTAG 1 cut(s) 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.