Rroxscaffold_2G00139210

Autophagy-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0007886
Physical Location & Seq
Forward (+)
77561519 .. 77565792
4274 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00139210.1

Sequence Viewer

Length: 552 bp
ATGAACTGCGAAGTTTGCCAACTCAAAGAATTGGAAGTCGAGCACTTTGAGATACGTGAAGTTCTGCGCTGTATACTTCACACAATTGTGTTCAATCGAGCTTTAGGTCTTGTGCGGCCGAAAGATGTTGATTTGGAACTTTTTGAAATTACATATGTGCAATGTGGAGATCTGGAACTTGAAAAGAAAATAGATGAGAAGATTGAGCAATTCATTAGTTGGGTAGAGAAACACCCGAACAAGAAAAGTCAGGTCTGCTTGTCTTTCTTTGAAGTAAAAAACAAACAGGCATCTTGGTTCAGTAACAAAACAGAGCGGCTATTTTGGGAACAGTGGTATATCAATTTAAATGTGGCCCAAAACCCAAAAGCACATTCTACCAAATCTCATCACTCCAAAGTTGTAGTTGATCCAGGAGAAAGTGCATCAGAGGAGAGGGTTGCTCGCAGAGCTGCACTTGAAGCAACTCTTCATGAAGTTTTGTTTCAAATAGTGAAATTTGTGAATGAGAAGAAGGATCACGTTCCCCCATACCGAATCTTGAGGGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04136 ko04140 ko04211 map04136 map04140 map04211
Pfam Domains
Protein Families
ATG101

Protein Analysis

183

Amino Acids

21.6

Weight (kDa)

6.71

Isoelectric Point (pI)

28.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG101 PF07855 10 - 177 2.9e-52 Autophagy-related protein 101
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 316
AccI GTMKAC 1 cut(s) 73
AciI CCGC 2 cut(s) 115, 316
AclWI GGATC 2 cut(s) 404, 525
AcoI YGGCCR 1 cut(s) 116
AcsI RAATTY 1 cut(s) 497
AgsI TTSAA 6 cut(s) 94, 146, 182, 272, 461, 488
AjnI CCWGG 1 cut(s) 412
AjuI GAANNNNNNNTTGG 2 cut(s) 358, 390
AleI CACNNNNGTG 1 cut(s) 86
AluBI AGCT 2 cut(s) 101, 452
AluI AGCT 2 cut(s) 101, 452
Alw21I GWGCWC 1 cut(s) 45
AlwI GGATC 2 cut(s) 404, 525
AoxI GGCC 2 cut(s) 116, 354
ApeKI GCWGC 1 cut(s) 452
ApoI RAATTY 1 cut(s) 497
AspLEI GCGC 1 cut(s) 69
AspS9I GGNCC 1 cut(s) 355
Bbv12I GWGCWC 1 cut(s) 45
BbvI GCAGC 1 cut(s) 439
BciT130I CCWGG 1 cut(s) 414
BglII AGATCT 1 cut(s) 169
BisI GCNGC 3 cut(s) 116, 317, 453
BlsI GCNGC 3 cut(s) 117, 318, 454
Bme1390I CCNGG 1 cut(s) 414
BmgT120I GGNCC 1 cut(s) 355
BmrFI CCNGG 1 cut(s) 414
BmsI GCATC 2 cut(s) 299, 434
BplI GAGNNNNNCTC 2 cut(s) 427, 459
BsaAI YACGTR 1 cut(s) 56
Bse3DI GCAATG 1 cut(s) 167
BseBI CCWGG 1 cut(s) 414
BseMI GCAATG 1 cut(s) 167
BseRI GAGGAG 1 cut(s) 446
BseX3I CGGCCG 1 cut(s) 116
BseXI GCAGC 1 cut(s) 439
BsgI GTGCAG 1 cut(s) 438
Bsh1285I CGRYCG 1 cut(s) 119
BshFI GGCC 2 cut(s) 118, 356
BsiEI CGRYCG 1 cut(s) 119
BsiHKAI GWGCWC 1 cut(s) 45
BsnI GGCC 2 cut(s) 118, 356
Bsp1286I GDGCHC 1 cut(s) 45
Bsp143I GATC 3 cut(s) 169, 409, 517
BspACI CCGC 2 cut(s) 115, 316
BspANI GGCC 2 cut(s) 118, 356
BspHI TCATGA 1 cut(s) 472
BspPI GGATC 2 cut(s) 404, 525
BsrBI CCGCTC 1 cut(s) 316
BsrDI GCAATG 1 cut(s) 167
BssMI GATC 3 cut(s) 169, 409, 517
BssNAI GTATAC 1 cut(s) 74
Bst1107I GTATAC 1 cut(s) 74
Bst2UI CCWGG 1 cut(s) 414
Bst4CI ACNGT 1 cut(s) 333
Bst6I CTCTTC 1 cut(s) 474
BstBAI YACGTR 1 cut(s) 56
BstC8I GCNNGC 1 cut(s) 445
BstHHI GCGC 1 cut(s) 69
BstKTI GATC 3 cut(s) 172, 412, 520
BstMBI GATC 3 cut(s) 169, 409, 517
BstMCI CGRYCG 1 cut(s) 119
BstMWI GCNNNNNNNGC 3 cut(s) 15, 449, 461
BstNI CCWGG 1 cut(s) 414
BstSCI CCNGG 1 cut(s) 412
BstV1I GCAGC 1 cut(s) 439
BstX2I RGATCY 1 cut(s) 169
BstYI RGATCY 1 cut(s) 169
BstZ17I GTATAC 1 cut(s) 74
BstZI CGGCCG 1 cut(s) 116
BsuRI GGCC 2 cut(s) 118, 356
BtsIMutI CAGTG 1 cut(s) 338
Cac8I GCNNGC 1 cut(s) 445
CciI TCATGA 1 cut(s) 472
CfoI GCGC 1 cut(s) 69
Cfr13I GGNCC 1 cut(s) 355
CviAII CATG 1 cut(s) 473
CviJI RGCY 5 cut(s) 101, 118, 319, 356, 452
CviKI_1 RGCY 5 cut(s) 101, 118, 319, 356, 452
DpnI GATC 3 cut(s) 171, 411, 519
DpnII GATC 3 cut(s) 169, 409, 517
DraI TTTAAA 1 cut(s) 348
EaeI YGGCCR 1 cut(s) 116
EagI CGGCCG 1 cut(s) 116
Eam1104I CTCTTC 1 cut(s) 474
EarI CTCTTC 1 cut(s) 474
EclXI CGGCCG 1 cut(s) 116
Eco52I CGGCCG 1 cut(s) 116
EcoRII CCWGG 1 cut(s) 412
FaeI CATG 1 cut(s) 476
FaiI YATR 6 cut(s) 74, 154, 156, 339, 474, 532
FalI AAGNNNNNCTT 2 cut(s) 453, 485
FatI CATG 1 cut(s) 472
FauNDI CATATG 1 cut(s) 154
FblI GTMKAC 1 cut(s) 73
Fnu4HI GCNGC 3 cut(s) 116, 317, 453
Fsp4HI GCNGC 3 cut(s) 116, 317, 453
GlaI GCGC 1 cut(s) 68
GluI GCNGC 3 cut(s) 116, 317, 453
HaeIII GGCC 2 cut(s) 118, 356
HhaI GCGC 1 cut(s) 69
Hin1II CATG 1 cut(s) 476
Hin6I GCGC 1 cut(s) 67
HinP1I GCGC 1 cut(s) 67
HinfI GANTC 1 cut(s) 537
Hpy166II GTNNAC 1 cut(s) 74
Hpy188I TCNGA 1 cut(s) 430
Hpy188III TCNNGA 3 cut(s) 173, 473, 541
Hpy8I GTNNAC 1 cut(s) 74
HpyAV CCTTC 1 cut(s) 508
HpyCH4III ACNGT 1 cut(s) 333
HpyCH4IV ACGT 2 cut(s) 55, 522
HpyCH4V TGCA 3 cut(s) 160, 425, 455
HpyF10VI GCNNNNNNNGC 3 cut(s) 15, 449, 461
HpySE526I ACGT 2 cut(s) 55, 522
Hsp92II CATG 1 cut(s) 476
HspAI GCGC 1 cut(s) 67
Kzo9I GATC 3 cut(s) 169, 409, 517
LpnPI CCDG 5 cut(s) 158, 236, 272, 399, 426
Lsp1109I GCAGC 1 cut(s) 439
LweI GCATC 2 cut(s) 299, 434
MaeII ACGT 2 cut(s) 55, 522
MaeIII GTNAC 1 cut(s) 302
MalI GATC 3 cut(s) 171, 411, 519
MbiI CCGCTC 1 cut(s) 316
MboI GATC 3 cut(s) 169, 409, 517
MboII GAAGA 3 cut(s) 211, 461, 523
MfeI CAATTG 1 cut(s) 84
MflI RGATCY 1 cut(s) 169
MhlI GDGCHC 1 cut(s) 45
MluCI AATT 6 cut(s) 29, 84, 147, 209, 343, 497
MnlI CCTC 3 cut(s) 424, 429, 537
MseI TTAA 1 cut(s) 347
MslI CAYNNNNRTG 1 cut(s) 86
MspR9I CCNGG 1 cut(s) 414
MunI CAATTG 1 cut(s) 84
MvaI CCWGG 1 cut(s) 414
MwoI GCNNNNNNNGC 3 cut(s) 15, 449, 461
NdeI CATATG 1 cut(s) 154
NdeII GATC 3 cut(s) 169, 409, 517
NlaIII CATG 1 cut(s) 476
OliI CACNNNNGTG 1 cut(s) 86
PagI TCATGA 1 cut(s) 472
PfeI GAWTC 1 cut(s) 537
PfoI TCCNGGA 1 cut(s) 412
PkrI GCNGC 3 cut(s) 117, 318, 454
Ppu21I YACGTR 1 cut(s) 56
Psp6I CCWGG 1 cut(s) 412
PspGI CCWGG 1 cut(s) 412
PspPI GGNCC 1 cut(s) 355
PsuI RGATCY 1 cut(s) 169
RseI CAYNNNNRTG 1 cut(s) 86
SaqAI TTAA 1 cut(s) 347
SatI GCNGC 3 cut(s) 116, 317, 453
Sau3AI GATC 3 cut(s) 169, 409, 517
Sau96I GGNCC 1 cut(s) 355
ScrFI CCNGG 1 cut(s) 414
SduI GDGCHC 1 cut(s) 45
SetI ASST 6 cut(s) 58, 103, 109, 255, 454, 525
SfaNI GCATC 2 cut(s) 299, 434
SmiI ATTTAAAT 1 cut(s) 348
SmiMI CAYNNNNRTG 1 cut(s) 86
SmlI CTYRAG 1 cut(s) 541
SmoI CTYRAG 1 cut(s) 541
Sse9I AATT 6 cut(s) 29, 84, 147, 209, 343, 497
SsiI CCGC 2 cut(s) 115, 316
StyD4I CCNGG 1 cut(s) 412
SwaI ATTTAAAT 1 cut(s) 348
TaaI ACNGT 1 cut(s) 333
TaiI ACGT 2 cut(s) 58, 525
TaqI TCGA 2 cut(s) 39, 97
TasI AATT 6 cut(s) 29, 84, 147, 209, 343, 497
TauI GCSGC 2 cut(s) 118, 319
TfiI GAWTC 1 cut(s) 537
Tru1I TTAA 1 cut(s) 347
Tru9I TTAA 1 cut(s) 347
TscAI CASTG 1 cut(s) 338
TseI GCWGC 1 cut(s) 452
TspDTI ATGAA 4 cut(s) 17, 202, 461, 489
TspRI CASTG 1 cut(s) 338
XapI RAATTY 1 cut(s) 497
XmiI GTMKAC 1 cut(s) 73
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.