MD02G1163200.v1.1

Autophagy-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0007886
Physical Location & Seq
Reverse (-)
13921092 .. 13925041
3950 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1163200.v1.1.491

Sequence Viewer

Length: 660 bp
ATGAACTGTGAAGTTTGCCAGCTCAAAGAATTGGAAGTCGAGCACTTTGAGATACGGGAAGTTCTGCGCTGCATACTTCACACAATTGTGTTTCATCGGGCTTTGGGTCTTGTGCGGCCCAAAGATGTTGATTTAGAACTTTTCGAAATTACATATGTGCAATGCGGAGATATTGAACTTGAGAAAAAAATAGATGAGAAGATTGAGCAATTCATTAGTTGGGTAGAGAAACACCCAAACAAGAAAAGTCAGGTCTGCTTGTCTTTCTTTGAAGAGAAAAACAAACAGGCAACTTGGTTTACGCACAAAACAGAAAGGTTATACTGGGAACAATGGTATATAAATTTGAATGTGGCCCAACACCTGAAAGTACATTCGGGCAAATCTCATAATTCTAAAGTTGTAGTTGATCCAGGAGAGAGTGCATTGGATGAGAGAATGGCTCGCAGAGCAGCACTTGAAGCATCTCTTCATGAGGTTTTGTTCCAAATAATAAAATTTGTGAATGAGAAGAAAGATCATGTTCCCCCGATACCACATACTGAGGGTGTAGTCTATTTCCCTCATGAAATTACCATCCCAAGTTCCTCGGATTCTGCATTTGGGATGGACATGATCAAGAGGATGCTGCATACTGGCCATCCAACCATGCTCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04136 ko04140 ko04211 map04136 map04140 map04211
Pfam Domains
Protein Families
ATG101

Protein Analysis

220

Amino Acids

25.61

Weight (kDa)

6.16

Isoelectric Point (pI)

31.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG101 PF07855 10 - 181 1.4e-54 Autophagy-related protein 101
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 115, 165
AclWI GGATC 1 cut(s) 404
AcoI YGGCCR 1 cut(s) 637
AcsI RAATTY 2 cut(s) 343, 497
AfaI GTAC 1 cut(s) 372
AgsI TTSAA 4 cut(s) 176, 272, 349, 461
AjnI CCWGG 1 cut(s) 412
AleI CACNNNNGTG 1 cut(s) 86
AluBI AGCT 2 cut(s) 22, 657
AluI AGCT 2 cut(s) 22, 657
Alw21I GWGCWC 1 cut(s) 45
AlwI GGATC 1 cut(s) 404
AoxI GGCC 3 cut(s) 116, 354, 637
ApeKI GCWGC 3 cut(s) 69, 452, 628
ApoI RAATTY 2 cut(s) 343, 497
AspLEI GCGC 1 cut(s) 69
AspS9I GGNCC 2 cut(s) 117, 355
AsuII TTCGAA 1 cut(s) 144
BalI TGGCCA 1 cut(s) 639
Bbv12I GWGCWC 1 cut(s) 45
BbvI GCAGC 3 cut(s) 56, 464, 615
BccI CCATC 3 cut(s) 584, 601, 648
BciT130I CCWGG 1 cut(s) 414
BclI TGATCA 1 cut(s) 615
BisI GCNGC 4 cut(s) 70, 116, 453, 629
BlpI GCTNAGC 1 cut(s) 653
BlsI GCNGC 4 cut(s) 71, 117, 454, 630
Bme1390I CCNGG 1 cut(s) 414
BmgT120I GGNCC 2 cut(s) 117, 355
BmrFI CCNGG 1 cut(s) 414
BmrI ACTGGG 1 cut(s) 334
BmsI GCATC 2 cut(s) 473, 615
BmuI ACTGGG 1 cut(s) 334
BplI GAGNNNNNCTC 2 cut(s) 427, 459
Bpu1102I GCTNAGC 1 cut(s) 653
Bpu14I TTCGAA 1 cut(s) 144
BpuEI CTTGAG 1 cut(s) 200
BsaJI CCNNGG 1 cut(s) 588
Bse1I ACTGG 2 cut(s) 329, 640
Bse3DI GCAATG 1 cut(s) 167
BseBI CCWGG 1 cut(s) 414
BseDI CCNNGG 1 cut(s) 588
BseGI GGATG 5 cut(s) 436, 576, 612, 630, 640
BseMI GCAATG 1 cut(s) 167
BseMII CTCAG 1 cut(s) 534
BseNI ACTGG 2 cut(s) 329, 640
BseXI GCAGC 3 cut(s) 56, 464, 615
BshFI GGCC 3 cut(s) 118, 356, 639
BsiHKAI GWGCWC 1 cut(s) 45
BsnI GGCC 3 cut(s) 118, 356, 639
Bsp119I TTCGAA 1 cut(s) 144
Bsp1286I GDGCHC 1 cut(s) 45
Bsp143I GATC 3 cut(s) 409, 517, 615
Bsp1720I GCTNAGC 1 cut(s) 653
BspACI CCGC 2 cut(s) 115, 165
BspANI GGCC 3 cut(s) 118, 356, 639
BspCNI CTCAG 1 cut(s) 535
BspHI TCATGA 2 cut(s) 472, 565
BspPI GGATC 1 cut(s) 404
BspT104I TTCGAA 1 cut(s) 144
BsrDI GCAATG 1 cut(s) 167
BsrI ACTGG 2 cut(s) 329, 640
BssECI CCNNGG 1 cut(s) 588
BssMI GATC 3 cut(s) 409, 517, 615
Bst2UI CCWGG 1 cut(s) 414
Bst4CI ACNGT 1 cut(s) 8
Bst6I CTCTTC 2 cut(s) 267, 474
BstBI TTCGAA 1 cut(s) 144
BstC8I GCNNGC 2 cut(s) 20, 445
BstDEI CTNAG 2 cut(s) 543, 653
BstF5I GGATG 5 cut(s) 436, 576, 612, 630, 640
BstHHI GCGC 1 cut(s) 69
BstKTI GATC 3 cut(s) 412, 520, 618
BstMBI GATC 3 cut(s) 409, 517, 615
BstMWI GCNNNNNNNGC 2 cut(s) 449, 461
BstNI CCWGG 1 cut(s) 414
BstSCI CCNGG 1 cut(s) 412
BstV1I GCAGC 3 cut(s) 56, 464, 615
BsuRI GGCC 3 cut(s) 118, 356, 639
BtsCI GGATG 5 cut(s) 436, 576, 612, 630, 640
Cac8I GCNNGC 2 cut(s) 20, 445
CciI TCATGA 2 cut(s) 472, 565
CfoI GCGC 1 cut(s) 69
Cfr13I GGNCC 2 cut(s) 117, 355
Csp6I GTAC 1 cut(s) 371
CviAII CATG 5 cut(s) 473, 521, 566, 613, 649
CviJI RGCY 7 cut(s) 22, 101, 118, 356, 443, 639, 657
CviKI_1 RGCY 7 cut(s) 22, 101, 118, 356, 443, 639, 657
CviQI GTAC 1 cut(s) 371
DdeI CTNAG 2 cut(s) 543, 653
DpnI GATC 3 cut(s) 411, 519, 617
DpnII GATC 3 cut(s) 409, 517, 615
EaeI YGGCCR 1 cut(s) 637
Eam1104I CTCTTC 2 cut(s) 267, 474
EarI CTCTTC 2 cut(s) 267, 474
EcoRII CCWGG 1 cut(s) 412
FaeI CATG 5 cut(s) 476, 524, 569, 616, 652
FalI AAGNNNNNCTT 2 cut(s) 453, 485
FatI CATG 5 cut(s) 472, 520, 565, 612, 648
FauNDI CATATG 1 cut(s) 154
FbaI TGATCA 1 cut(s) 615
Fnu4HI GCNGC 4 cut(s) 70, 116, 453, 629
FokI GGATG 5 cut(s) 443, 563, 619, 627, 637
Fsp4HI GCNGC 4 cut(s) 70, 116, 453, 629
GlaI GCGC 1 cut(s) 68
GluI GCNGC 4 cut(s) 70, 116, 453, 629
HaeIII GGCC 3 cut(s) 118, 356, 639
HhaI GCGC 1 cut(s) 69
Hin1II CATG 5 cut(s) 476, 524, 569, 616, 652
Hin6I GCGC 1 cut(s) 67
HinP1I GCGC 1 cut(s) 67
HinfI GANTC 1 cut(s) 593
Hpy166II GTNNAC 1 cut(s) 300
Hpy188I TCNGA 1 cut(s) 592
Hpy188III TCNNGA 3 cut(s) 473, 566, 619
Hpy8I GTNNAC 1 cut(s) 300
HpyCH4III ACNGT 1 cut(s) 8
HpyCH4V TGCA 5 cut(s) 72, 160, 425, 599, 631
HpyF10VI GCNNNNNNNGC 2 cut(s) 449, 461
HpyF3I CTNAG 2 cut(s) 543, 653
Hsp92II CATG 5 cut(s) 476, 524, 569, 616, 652
HspAI GCGC 1 cut(s) 67
Ksp22I TGATCA 1 cut(s) 615
Kzo9I GATC 3 cut(s) 409, 517, 615
LpnPI CCDG 8 cut(s) 32, 236, 272, 310, 377, 399, 426, 621
Lsp1109I GCAGC 3 cut(s) 56, 464, 615
LweI GCATC 2 cut(s) 473, 615
MalI GATC 3 cut(s) 411, 519, 617
MboI GATC 3 cut(s) 409, 517, 615
MboII GAAGA 4 cut(s) 211, 284, 461, 523
MfeI CAATTG 1 cut(s) 84
MhlI GDGCHC 1 cut(s) 45
MlsI TGGCCA 1 cut(s) 639
MluCI AATT 8 cut(s) 29, 84, 147, 209, 343, 391, 497, 570
MluNI TGGCCA 1 cut(s) 639
MnlI CCTC 5 cut(s) 469, 538, 573, 598, 615
Mox20I TGGCCA 1 cut(s) 639
MscI TGGCCA 1 cut(s) 639
MslI CAYNNNNRTG 1 cut(s) 86
Msp20I TGGCCA 1 cut(s) 639
MspA1I CMGCKG 1 cut(s) 657
MspR9I CCNGG 1 cut(s) 414
MunI CAATTG 1 cut(s) 84
MvaI CCWGG 1 cut(s) 414
MwoI GCNNNNNNNGC 2 cut(s) 449, 461
NdeI CATATG 1 cut(s) 154
NdeII GATC 3 cut(s) 409, 517, 615
NlaIII CATG 5 cut(s) 476, 524, 569, 616, 652
NspV TTCGAA 1 cut(s) 144
OliI CACNNNNGTG 1 cut(s) 86
PagI TCATGA 2 cut(s) 472, 565
PfeI GAWTC 1 cut(s) 593
PfoI TCCNGGA 1 cut(s) 412
PkrI GCNGC 4 cut(s) 71, 117, 454, 630
Psp6I CCWGG 1 cut(s) 412
PspGI CCWGG 1 cut(s) 412
PspPI GGNCC 2 cut(s) 117, 355
PvuII CAGCTG 1 cut(s) 657
RsaI GTAC 1 cut(s) 372
RsaNI GTAC 1 cut(s) 371
RseI CAYNNNNRTG 1 cut(s) 86
SatI GCNGC 4 cut(s) 70, 116, 453, 629
Sau3AI GATC 3 cut(s) 409, 517, 615
Sau96I GGNCC 2 cut(s) 117, 355
ScrFI CCNGG 1 cut(s) 414
SduI GDGCHC 1 cut(s) 45
SetI ASST 6 cut(s) 24, 255, 320, 366, 480, 659
SfaNI GCATC 2 cut(s) 473, 615
SfuI TTCGAA 1 cut(s) 144
SmiMI CAYNNNNRTG 1 cut(s) 86
SmlI CTYRAG 1 cut(s) 179
SmoI CTYRAG 1 cut(s) 179
Sse9I AATT 8 cut(s) 29, 84, 147, 209, 343, 391, 497, 570
SsiI CCGC 2 cut(s) 115, 165
StyD4I CCNGG 1 cut(s) 412
TaaI ACNGT 1 cut(s) 8
TaqI TCGA 2 cut(s) 39, 144
TasI AATT 8 cut(s) 29, 84, 147, 209, 343, 391, 497, 570
TatI WGTACW 1 cut(s) 370
TauI GCSGC 1 cut(s) 118
TfiI GAWTC 1 cut(s) 593
TseI GCWGC 3 cut(s) 69, 452, 628
TspDTI ATGAA 5 cut(s) 17, 83, 202, 461, 582
XapI RAATTY 2 cut(s) 343, 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.