FvH4_1g14960

Autophagy-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0007886
Physical Location & Seq
Reverse (-)
8396758 .. 8400451
3694 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g14960.t2

Sequence Viewer

Length: 600 bp
ATGAACTGCGAAGTTTGCCAACTCAAAGAATTGGAAGTCGAGCACTTTGAGATTCGTGAAGTTCTTCGCTGCATACTTCACACAATTGTGTTCAACCGGGCTTTAGGTCTTGTTCGGCCGAAAGATGTTGATTTGGAACTATTTGAAATTACATATGTGCAATGTGGAGATGTGGATCTTGAAAAGAAAATAGATGAGAAGATTGAGCAATTCATCAGTTGGGTAGAGAAACACCCAAACAAGAAAAGTCAGGTCTGCTTGTCTTTCTTTGAAGTAAAAAAAAACAAACAGGCATCTTGGTTCACTAATAAGACAGAGCGGCTATATTGGGAACAGTGGTATATCAATTTAAATGTGGCCCAAACCCCAAAAGTACATTCTACCAAATCTCATCACTCCAAAATTGTAGTTGATCCAGGAGAAAGTGCATCAGAGGAGAGAATTGCTCGCAGAGCTGCACTTGAATCAACTCTTCATGAGGTTTTGTTTCAAATTGTGAAATTTGTGAACGAGAAGAAAGATCATGTTCCCCCCATACCGAATCTTGAGGGTGTAGTCTATTTCCCCCATGAAATAACTATCCCAAGGAAGTTTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04136 ko04140 ko04211 map04136 map04140 map04211
Pfam Domains
Protein Families
ATG101

Protein Analysis

200

Amino Acids

23.45

Weight (kDa)

6.51

Isoelectric Point (pI)

25.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG101 PF07855 10 - 181 1.6e-53 Autophagy-related protein 101
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 319
AciI CCGC 1 cut(s) 319
AclWI GGATC 2 cut(s) 183, 407
AcoI YGGCCR 1 cut(s) 116
AcsI RAATTY 1 cut(s) 500
AfaI GTAC 1 cut(s) 375
AgsI TTSAA 6 cut(s) 94, 146, 182, 272, 464, 491
AjnI CCWGG 1 cut(s) 415
AjuI GAANNNNNNNTTGG 2 cut(s) 361, 393
AleI CACNNNNGTG 1 cut(s) 86
AluBI AGCT 1 cut(s) 455
AluI AGCT 1 cut(s) 455
Alw21I GWGCWC 1 cut(s) 45
AlwI GGATC 2 cut(s) 183, 407
AoxI GGCC 2 cut(s) 116, 357
ApeKI GCWGC 2 cut(s) 69, 455
ApoI RAATTY 1 cut(s) 500
Asp700I GAANNNNTTC 1 cut(s) 63
AspS9I GGNCC 1 cut(s) 358
AsuC2I CCSGG 1 cut(s) 98
Bbv12I GWGCWC 1 cut(s) 45
BbvI GCAGC 2 cut(s) 56, 442
BciT130I CCWGG 1 cut(s) 417
BcnI CCSGG 1 cut(s) 98
BisI GCNGC 3 cut(s) 70, 320, 456
BlsI GCNGC 3 cut(s) 71, 321, 457
Bme1390I CCNGG 2 cut(s) 98, 417
BmgT120I GGNCC 1 cut(s) 358
BmrFI CCNGG 2 cut(s) 98, 417
BmsI GCATC 2 cut(s) 302, 437
BplI GAGNNNNNCTC 2 cut(s) 430, 462
BpuEI CTTGAG 1 cut(s) 566
BpuMI CCSGG 1 cut(s) 98
BsaBI GATNNNNATC 1 cut(s) 174
BsaJI CCNNGG 1 cut(s) 584
Bse3DI GCAATG 1 cut(s) 167
Bse8I GATNNNNATC 1 cut(s) 174
BseBI CCWGG 1 cut(s) 417
BseDI CCNNGG 1 cut(s) 584
BseJI GATNNNNATC 1 cut(s) 174
BseMI GCAATG 1 cut(s) 167
BseRI GAGGAG 1 cut(s) 449
BseX3I CGGCCG 1 cut(s) 116
BseXI GCAGC 2 cut(s) 56, 442
BsgI GTGCAG 1 cut(s) 441
Bsh1285I CGRYCG 1 cut(s) 119
BshFI GGCC 2 cut(s) 118, 359
BsiEI CGRYCG 1 cut(s) 119
BsiHKAI GWGCWC 1 cut(s) 45
BsiSI CCGG 1 cut(s) 97
BsnI GGCC 2 cut(s) 118, 359
Bsp1286I GDGCHC 1 cut(s) 45
Bsp143I GATC 3 cut(s) 175, 412, 520
BspACI CCGC 1 cut(s) 319
BspANI GGCC 2 cut(s) 118, 359
BspHI TCATGA 1 cut(s) 475
BspPI GGATC 2 cut(s) 183, 407
BsrBI CCGCTC 1 cut(s) 319
BsrDI GCAATG 1 cut(s) 167
BssECI CCNNGG 1 cut(s) 584
BssMI GATC 3 cut(s) 175, 412, 520
BssT1I CCWWGG 1 cut(s) 584
Bst2UI CCWGG 1 cut(s) 417
Bst4CI ACNGT 1 cut(s) 336
Bst6I CTCTTC 1 cut(s) 477
BstC8I GCNNGC 1 cut(s) 448
BstKTI GATC 3 cut(s) 178, 415, 523
BstMBI GATC 3 cut(s) 175, 412, 520
BstMCI CGRYCG 1 cut(s) 119
BstMWI GCNNNNNNNGC 2 cut(s) 15, 452
BstNI CCWGG 1 cut(s) 417
BstSCI CCNGG 2 cut(s) 96, 415
BstV1I GCAGC 2 cut(s) 56, 442
BstX2I RGATCY 1 cut(s) 175
BstYI RGATCY 1 cut(s) 175
BstZI CGGCCG 1 cut(s) 116
BsuRI GGCC 2 cut(s) 118, 359
BtsIMutI CAGTG 1 cut(s) 341
Cac8I GCNNGC 1 cut(s) 448
CciI TCATGA 1 cut(s) 475
Cfr13I GGNCC 1 cut(s) 358
Csp6I GTAC 1 cut(s) 374
CviAII CATG 3 cut(s) 476, 524, 569
CviJI RGCY 5 cut(s) 101, 118, 322, 359, 455
CviKI_1 RGCY 5 cut(s) 101, 118, 322, 359, 455
CviQI GTAC 1 cut(s) 374
DpnI GATC 3 cut(s) 177, 414, 522
DpnII GATC 3 cut(s) 175, 412, 520
DraI TTTAAA 1 cut(s) 351
EaeI YGGCCR 1 cut(s) 116
EagI CGGCCG 1 cut(s) 116
Eam1104I CTCTTC 1 cut(s) 477
EarI CTCTTC 1 cut(s) 477
EclXI CGGCCG 1 cut(s) 116
Eco130I CCWWGG 1 cut(s) 584
Eco52I CGGCCG 1 cut(s) 116
EcoRII CCWGG 1 cut(s) 415
EcoT14I CCWWGG 1 cut(s) 584
ErhI CCWWGG 1 cut(s) 584
FaeI CATG 3 cut(s) 479, 527, 572
FaiI YATR 9 cut(s) 74, 154, 156, 325, 342, 477, 525, 536, 570
FatI CATG 3 cut(s) 475, 523, 568
FauNDI CATATG 1 cut(s) 154
Fnu4HI GCNGC 3 cut(s) 70, 320, 456
Fsp4HI GCNGC 3 cut(s) 70, 320, 456
GluI GCNGC 3 cut(s) 70, 320, 456
HaeIII GGCC 2 cut(s) 118, 359
HapII CCGG 1 cut(s) 97
Hin1II CATG 3 cut(s) 479, 527, 572
HinfI GANTC 3 cut(s) 52, 464, 541
HpaII CCGG 1 cut(s) 97
Hpy166II GTNNAC 2 cut(s) 303, 508
Hpy188I TCNGA 2 cut(s) 433, 599
Hpy188III TCNNGA 4 cut(s) 56, 179, 476, 545
Hpy8I GTNNAC 2 cut(s) 303, 508
HpyCH4III ACNGT 1 cut(s) 336
HpyCH4V TGCA 4 cut(s) 72, 160, 428, 458
HpyF10VI GCNNNNNNNGC 2 cut(s) 15, 452
Hsp92II CATG 3 cut(s) 479, 527, 572
Kzo9I GATC 3 cut(s) 175, 412, 520
LpnPI CCDG 5 cut(s) 110, 236, 275, 402, 429
Lsp1109I GCAGC 2 cut(s) 56, 442
LweI GCATC 2 cut(s) 302, 437
MalI GATC 3 cut(s) 177, 414, 522
MbiI CCGCTC 1 cut(s) 319
MboI GATC 3 cut(s) 175, 412, 520
MboII GAAGA 4 cut(s) 56, 211, 464, 526
MfeI CAATTG 1 cut(s) 84
MflI RGATCY 1 cut(s) 175
MhlI GDGCHC 1 cut(s) 45
MluCI AATT 9 cut(s) 29, 84, 147, 209, 346, 402, 441, 492, 500
MnlI CCTC 3 cut(s) 427, 472, 541
MroXI GAANNNNTTC 1 cut(s) 63
MseI TTAA 1 cut(s) 350
MslI CAYNNNNRTG 1 cut(s) 86
MspI CCGG 1 cut(s) 97
MspR9I CCNGG 2 cut(s) 98, 417
MunI CAATTG 1 cut(s) 84
MvaI CCWGG 1 cut(s) 417
MwoI GCNNNNNNNGC 2 cut(s) 15, 452
NciI CCSGG 1 cut(s) 98
NdeI CATATG 1 cut(s) 154
NdeII GATC 3 cut(s) 175, 412, 520
NlaIII CATG 3 cut(s) 479, 527, 572
OliI CACNNNNGTG 1 cut(s) 86
PagI TCATGA 1 cut(s) 475
PdmI GAANNNNTTC 1 cut(s) 63
PfeI GAWTC 3 cut(s) 52, 464, 541
PfoI TCCNGGA 1 cut(s) 415
PkrI GCNGC 3 cut(s) 71, 321, 457
Psp6I CCWGG 1 cut(s) 415
PspGI CCWGG 1 cut(s) 415
PspPI GGNCC 1 cut(s) 358
PsuI RGATCY 1 cut(s) 175
RsaI GTAC 1 cut(s) 375
RsaNI GTAC 1 cut(s) 374
RseI CAYNNNNRTG 1 cut(s) 86
SaqAI TTAA 1 cut(s) 350
SatI GCNGC 3 cut(s) 70, 320, 456
Sau3AI GATC 3 cut(s) 175, 412, 520
Sau96I GGNCC 1 cut(s) 358
ScrFI CCNGG 2 cut(s) 98, 417
SduI GDGCHC 1 cut(s) 45
SetI ASST 4 cut(s) 109, 255, 457, 483
SfaNI GCATC 2 cut(s) 302, 437
SmiI ATTTAAAT 1 cut(s) 351
SmiMI CAYNNNNRTG 1 cut(s) 86
SmlI CTYRAG 1 cut(s) 545
SmoI CTYRAG 1 cut(s) 545
Sse9I AATT 9 cut(s) 29, 84, 147, 209, 346, 402, 441, 492, 500
SsiI CCGC 1 cut(s) 319
StyD4I CCNGG 2 cut(s) 96, 415
StyI CCWWGG 1 cut(s) 584
SwaI ATTTAAAT 1 cut(s) 351
TaaI ACNGT 1 cut(s) 336
TaqI TCGA 1 cut(s) 39
TasI AATT 9 cut(s) 29, 84, 147, 209, 346, 402, 441, 492, 500
TatI WGTACW 1 cut(s) 373
TauI GCSGC 1 cut(s) 322
TfiI GAWTC 3 cut(s) 52, 464, 541
Tru1I TTAA 1 cut(s) 350
Tru9I TTAA 1 cut(s) 350
TscAI CASTG 1 cut(s) 341
TseI GCWGC 2 cut(s) 69, 455
TspDTI ATGAA 4 cut(s) 17, 202, 464, 585
TspRI CASTG 1 cut(s) 341
XapI RAATTY 1 cut(s) 500
XmnI GAANNNNTTC 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.