MD15G1276200.v1.1

Autophagy-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0007886
Physical Location & Seq
Reverse (-)
24243809 .. 24247570
3762 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1276200.v1.1.491

Sequence Viewer

Length: 651 bp
ATGAACTGTGAAGTTTGCCAGCTCAAAGAATTGGAAGTCGAGCACTTTGAGATACGGGAAGTTCTGCGCTGCATACTTCACACAATTGTGTTTCATCGGACATTAGGTCTTGTGCGGCCCAAGGATATTGATTTGGAACTTTTCGAAATTACATATGTGCAATGTGGAGATGTTGAACTTGAGAAGAAAATAGATGAGAAGATTGAGCAATTCATTAGTTGGGTAGAGAAACACCCAAACAAGAAAAGTCAGATCTGCTTGTCTTTCTTTGAACTGAAAAACAAACAGGCAACTTGGTTTACACACAAAACAGAAAGGTTATACTGGGAACAATGGTATATAAATTTGAATGTGGCCCAACACCCCAAAGCACAATCTGGCAATTCTCATAATTCTAAAGTTGTAGGTGATCCAGGAGAGAGTGCATTGGACGAGAGAATGGCTCGCAGAGCAGCACTTGAAGCATCTCTTCGTGAGGTTTTGTTCCAAGTAATAAAATTTGTGAATGAGAAGAAAGATCATGTTCCAAGTATTGAGGGTGTAGTCTCTTTCCCTCATGAAATTACTATCCCAAGTTCCTCGGATTCTGCATTTGGGATGGACATGATCAAGAGGATGCTGCAGACTGGCCATCCAACCATGCTGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04136 ko04140 ko04211 map04136 map04140 map04211
Pfam Domains
Protein Families
ATG101

Protein Analysis

217

Amino Acids

25.11

Weight (kDa)

6.07

Isoelectric Point (pI)

36.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG101 PF07855 10 - 178 4.9e-54 Autophagy-related protein 101
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 115
AclWI GGATC 1 cut(s) 404
AcoI YGGCCR 1 cut(s) 628
AcsI RAATTY 2 cut(s) 343, 497
AgsI TTSAA 4 cut(s) 176, 272, 349, 461
AhdI GACNNNNNGTC 1 cut(s) 105
AjnI CCWGG 1 cut(s) 412
AleI CACNNNNGTG 1 cut(s) 86
AluBI AGCT 2 cut(s) 22, 648
AluI AGCT 2 cut(s) 22, 648
Alw21I GWGCWC 1 cut(s) 45
Alw26I GTCTC 1 cut(s) 550
AlwI GGATC 1 cut(s) 404
AoxI GGCC 3 cut(s) 116, 354, 628
ApeKI GCWGC 3 cut(s) 69, 452, 619
ApoI RAATTY 2 cut(s) 343, 497
AspLEI GCGC 1 cut(s) 69
AspS9I GGNCC 2 cut(s) 117, 355
AsuHPI GGTGA 1 cut(s) 419
AsuII TTCGAA 1 cut(s) 144
BalI TGGCCA 1 cut(s) 630
Bbv12I GWGCWC 1 cut(s) 45
BbvI GCAGC 3 cut(s) 56, 464, 606
BccI CCATC 2 cut(s) 592, 639
BciT130I CCWGG 1 cut(s) 414
BclI TGATCA 1 cut(s) 606
BcoDI GTCTC 1 cut(s) 550
BfmI CTRYAG 1 cut(s) 620
BglII AGATCT 1 cut(s) 252
BisI GCNGC 4 cut(s) 70, 116, 453, 620
BlpI GCTNAGC 1 cut(s) 644
BlsI GCNGC 4 cut(s) 71, 117, 454, 621
Bme1390I CCNGG 1 cut(s) 414
BmeRI GACNNNNNGTC 1 cut(s) 105
BmgT120I GGNCC 2 cut(s) 117, 355
BmrFI CCNGG 1 cut(s) 414
BmrI ACTGGG 1 cut(s) 334
BmsI GCATC 2 cut(s) 473, 606
BmuI ACTGGG 1 cut(s) 334
BplI GAGNNNNNCTC 2 cut(s) 427, 459
Bpu1102I GCTNAGC 1 cut(s) 644
Bpu14I TTCGAA 1 cut(s) 144
BpuEI CTTGAG 1 cut(s) 200
BsaJI CCNNGG 2 cut(s) 120, 579
Bse1I ACTGG 2 cut(s) 329, 631
Bse3DI GCAATG 1 cut(s) 167
BseBI CCWGG 1 cut(s) 414
BseDI CCNNGG 2 cut(s) 120, 579
BseGI GGATG 3 cut(s) 603, 621, 631
BseMI GCAATG 1 cut(s) 167
BseMII CTCAG 1 cut(s) 635
BseNI ACTGG 2 cut(s) 329, 631
BseXI GCAGC 3 cut(s) 56, 464, 606
BshFI GGCC 3 cut(s) 118, 356, 630
BsiHKAI GWGCWC 1 cut(s) 45
BsmAI GTCTC 1 cut(s) 550
BsnI GGCC 3 cut(s) 118, 356, 630
Bsp119I TTCGAA 1 cut(s) 144
Bsp1286I GDGCHC 1 cut(s) 45
Bsp143I GATC 4 cut(s) 252, 409, 517, 606
Bsp1720I GCTNAGC 1 cut(s) 644
BspACI CCGC 1 cut(s) 115
BspANI GGCC 3 cut(s) 118, 356, 630
BspCNI CTCAG 1 cut(s) 636
BspHI TCATGA 1 cut(s) 556
BspMAI CTGCAG 1 cut(s) 624
BspPI GGATC 1 cut(s) 404
BspT104I TTCGAA 1 cut(s) 144
BsrDI GCAATG 1 cut(s) 167
BsrI ACTGG 2 cut(s) 329, 631
BssECI CCNNGG 2 cut(s) 120, 579
BssMI GATC 4 cut(s) 252, 409, 517, 606
BssT1I CCWWGG 1 cut(s) 120
Bst2UI CCWGG 1 cut(s) 414
Bst4CI ACNGT 1 cut(s) 8
Bst6I CTCTTC 1 cut(s) 474
BstBI TTCGAA 1 cut(s) 144
BstC8I GCNNGC 2 cut(s) 20, 445
BstDEI CTNAG 1 cut(s) 644
BstF5I GGATG 3 cut(s) 603, 621, 631
BstHHI GCGC 1 cut(s) 69
BstKTI GATC 4 cut(s) 255, 412, 520, 609
BstMAI GTCTC 1 cut(s) 550
BstMBI GATC 4 cut(s) 252, 409, 517, 606
BstMWI GCNNNNNNNGC 2 cut(s) 449, 461
BstNI CCWGG 1 cut(s) 414
BstSCI CCNGG 1 cut(s) 412
BstSFI CTRYAG 1 cut(s) 620
BstV1I GCAGC 3 cut(s) 56, 464, 606
BstX2I RGATCY 1 cut(s) 252
BstYI RGATCY 1 cut(s) 252
BsuRI GGCC 3 cut(s) 118, 356, 630
BtsCI GGATG 3 cut(s) 603, 621, 631
Cac8I GCNNGC 2 cut(s) 20, 445
CciI TCATGA 1 cut(s) 556
CfoI GCGC 1 cut(s) 69
Cfr13I GGNCC 2 cut(s) 117, 355
CviAII CATG 4 cut(s) 521, 557, 604, 640
CviJI RGCY 6 cut(s) 22, 118, 356, 443, 630, 648
CviKI_1 RGCY 6 cut(s) 22, 118, 356, 443, 630, 648
DdeI CTNAG 1 cut(s) 644
DpnI GATC 4 cut(s) 254, 411, 519, 608
DpnII GATC 4 cut(s) 252, 409, 517, 606
DriI GACNNNNNGTC 1 cut(s) 105
EaeI YGGCCR 1 cut(s) 628
Eam1104I CTCTTC 1 cut(s) 474
Eam1105I GACNNNNNGTC 1 cut(s) 105
EarI CTCTTC 1 cut(s) 474
Eco130I CCWWGG 1 cut(s) 120
EcoRII CCWGG 1 cut(s) 412
EcoT14I CCWWGG 1 cut(s) 120
ErhI CCWWGG 1 cut(s) 120
FaeI CATG 4 cut(s) 524, 560, 607, 643
FalI AAGNNNNNCTT 2 cut(s) 453, 485
FatI CATG 4 cut(s) 520, 556, 603, 639
FauNDI CATATG 1 cut(s) 154
FbaI TGATCA 1 cut(s) 606
Fnu4HI GCNGC 4 cut(s) 70, 116, 453, 620
FokI GGATG 3 cut(s) 610, 618, 628
Fsp4HI GCNGC 4 cut(s) 70, 116, 453, 620
GlaI GCGC 1 cut(s) 68
GluI GCNGC 4 cut(s) 70, 116, 453, 620
HaeIII GGCC 3 cut(s) 118, 356, 630
HhaI GCGC 1 cut(s) 69
Hin1II CATG 4 cut(s) 524, 560, 607, 643
Hin6I GCGC 1 cut(s) 67
HinP1I GCGC 1 cut(s) 67
HinfI GANTC 1 cut(s) 584
HphI GGTGA 1 cut(s) 419
Hpy166II GTNNAC 1 cut(s) 300
Hpy188I TCNGA 3 cut(s) 99, 252, 583
Hpy188III TCNNGA 3 cut(s) 473, 557, 610
Hpy8I GTNNAC 1 cut(s) 300
HpyCH4III ACNGT 1 cut(s) 8
HpyCH4V TGCA 5 cut(s) 72, 160, 425, 590, 622
HpyF10VI GCNNNNNNNGC 2 cut(s) 449, 461
HpyF3I CTNAG 1 cut(s) 644
Hsp92II CATG 4 cut(s) 524, 560, 607, 643
HspAI GCGC 1 cut(s) 67
Ksp22I TGATCA 1 cut(s) 606
Kzo9I GATC 4 cut(s) 252, 409, 517, 606
LpnPI CCDG 7 cut(s) 32, 272, 310, 363, 399, 426, 612
Lsp1109I GCAGC 3 cut(s) 56, 464, 606
LweI GCATC 2 cut(s) 473, 606
MalI GATC 4 cut(s) 254, 411, 519, 608
MboI GATC 4 cut(s) 252, 409, 517, 606
MboII GAAGA 4 cut(s) 196, 211, 461, 523
MfeI CAATTG 1 cut(s) 84
MflI RGATCY 1 cut(s) 252
MhlI GDGCHC 1 cut(s) 45
MlsI TGGCCA 1 cut(s) 630
MluCI AATT 9 cut(s) 29, 84, 147, 209, 343, 382, 391, 497, 561
MluNI TGGCCA 1 cut(s) 630
MnlI CCTC 5 cut(s) 469, 529, 564, 589, 606
Mox20I TGGCCA 1 cut(s) 630
MscI TGGCCA 1 cut(s) 630
MslI CAYNNNNRTG 1 cut(s) 86
Msp20I TGGCCA 1 cut(s) 630
MspR9I CCNGG 1 cut(s) 414
MunI CAATTG 1 cut(s) 84
MvaI CCWGG 1 cut(s) 414
MwoI GCNNNNNNNGC 2 cut(s) 449, 461
NdeI CATATG 1 cut(s) 154
NdeII GATC 4 cut(s) 252, 409, 517, 606
NlaIII CATG 4 cut(s) 524, 560, 607, 643
NspV TTCGAA 1 cut(s) 144
OliI CACNNNNGTG 1 cut(s) 86
PagI TCATGA 1 cut(s) 556
PfeI GAWTC 1 cut(s) 584
PfoI TCCNGGA 1 cut(s) 412
PkrI GCNGC 4 cut(s) 71, 117, 454, 621
Psp6I CCWGG 1 cut(s) 412
PspGI CCWGG 1 cut(s) 412
PspPI GGNCC 2 cut(s) 117, 355
PstI CTGCAG 1 cut(s) 624
PsuI RGATCY 1 cut(s) 252
RseI CAYNNNNRTG 1 cut(s) 86
SatI GCNGC 4 cut(s) 70, 116, 453, 620
Sau3AI GATC 4 cut(s) 252, 409, 517, 606
Sau96I GGNCC 2 cut(s) 117, 355
ScrFI CCNGG 1 cut(s) 414
SduI GDGCHC 1 cut(s) 45
SetI ASST 6 cut(s) 24, 109, 320, 409, 480, 650
SfaNI GCATC 2 cut(s) 473, 606
SfcI CTRYAG 1 cut(s) 620
SfuI TTCGAA 1 cut(s) 144
SmiMI CAYNNNNRTG 1 cut(s) 86
SmlI CTYRAG 1 cut(s) 179
SmoI CTYRAG 1 cut(s) 179
Sse9I AATT 9 cut(s) 29, 84, 147, 209, 343, 382, 391, 497, 561
SsiI CCGC 1 cut(s) 115
StyD4I CCNGG 1 cut(s) 412
StyI CCWWGG 1 cut(s) 120
TaaI ACNGT 1 cut(s) 8
TaqI TCGA 2 cut(s) 39, 144
TasI AATT 9 cut(s) 29, 84, 147, 209, 343, 382, 391, 497, 561
TauI GCSGC 1 cut(s) 118
TfiI GAWTC 1 cut(s) 584
TseI GCWGC 3 cut(s) 69, 452, 619
TspDTI ATGAA 4 cut(s) 17, 83, 202, 573
XapI RAATTY 2 cut(s) 343, 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.