RchiOBHm_Chr2g0104011

Autophagy-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0007886
Physical Location & Seq
Reverse (-)
15390896 .. 15394614
3719 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47830

Sequence Viewer

Length: 660 bp
ATGAACTGCGAAGTTTGCCAACTCAAAGAATTGGAAGTCGAGCACTTTGAGATACGTGAAGTTCTGCGCTGCATACTTCACACAATTGTGTTCAATCGAGCTTTAGGTCTCGTGCGGCCGAAAGATGTTGATTTGGAACTTTTTGAAATAACATATGTGCAATGTGGAGATCTGGAACTTGAAAAGAAAATAGATGAAAAGATTGAGCAATTCATTAGTTGGGTAGAGAAACACCCGAACAAGAAAAGTCAGGTCTGCTTGTCTTTCTTTGAAGTAAAAAACAAACAGCCATCTTGGTTCACTAACAAAACAGAGCGGCTGTATTGGGAACAGTGGTATATCAATTTGAATGTGGCCCAAAACCCAAAAGCACATTCTACCAAATCTCATCACTCCAAAGTTGTAGTTGATCCAGGAGAAAGTGCATCAGAGGAGAGGGTTGCTCGCAGAGCTGCACTTGAAGCAACTCTTCATGAAGTTTTGTTTCAAATAGTGAAATTTGTGAATGAGAAGAAAGATCACGTTCCCCCCATACCGAATCTTGAGGGTGTAGTCTCTTTCCCGTATGAAATAACTATCCCAAGTTCCTCGGACTCCGCATTTGGGATGGACATGATCAAGAGGATGCTGCAGACTGGCCATCCAACCATGCTTAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04136 ko04140 ko04211 map04136 map04140 map04211
Pfam Domains
Protein Families
ATG101

Protein Analysis

219

Amino Acids

25.44

Weight (kDa)

6.01

Isoelectric Point (pI)

32.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG101 PF07855 10 - 180 7.7e-54 Autophagy-related protein 101
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 316
AciI CCGC 3 cut(s) 115, 316, 597
AclWI GGATC 1 cut(s) 404
AcoI YGGCCR 2 cut(s) 116, 637
AcsI RAATTY 1 cut(s) 497
AfiI CCNNNNNNNGG 1 cut(s) 603
AgsI TTSAA 7 cut(s) 94, 146, 182, 272, 349, 461, 488
AjnI CCWGG 1 cut(s) 412
AjuI GAANNNNNNNTTGG 2 cut(s) 358, 390
AleI CACNNNNGTG 1 cut(s) 86
AluBI AGCT 3 cut(s) 101, 452, 657
AluI AGCT 3 cut(s) 101, 452, 657
Alw21I GWGCWC 1 cut(s) 45
Alw26I GTCTC 2 cut(s) 113, 559
AlwI GGATC 1 cut(s) 404
AoxI GGCC 3 cut(s) 116, 354, 637
ApeKI GCWGC 3 cut(s) 69, 452, 628
ApoI RAATTY 1 cut(s) 497
ArsI GACNNNNNNTTYG 2 cut(s) 584, 616
AspLEI GCGC 1 cut(s) 69
AspS9I GGNCC 1 cut(s) 355
BalI TGGCCA 1 cut(s) 639
BauI CACGAG 1 cut(s) 110
Bbv12I GWGCWC 1 cut(s) 45
BbvI GCAGC 3 cut(s) 56, 439, 615
BccI CCATC 3 cut(s) 298, 601, 648
BciT130I CCWGG 1 cut(s) 414
BclI TGATCA 1 cut(s) 615
BcoDI GTCTC 2 cut(s) 113, 559
BfmI CTRYAG 1 cut(s) 629
BglII AGATCT 1 cut(s) 169
BisI GCNGC 5 cut(s) 70, 116, 317, 453, 629
BlpI GCTNAGC 1 cut(s) 653
BlsI GCNGC 5 cut(s) 71, 117, 318, 454, 630
Bme1390I CCNGG 1 cut(s) 414
BmgT120I GGNCC 1 cut(s) 355
BmrFI CCNGG 1 cut(s) 414
BmsI GCATC 2 cut(s) 434, 615
BplI GAGNNNNNCTC 2 cut(s) 427, 459
Bpu1102I GCTNAGC 1 cut(s) 653
BpuEI CTTGAG 1 cut(s) 563
BsaAI YACGTR 1 cut(s) 56
BsaI GGTCTC 1 cut(s) 113
BsaJI CCNNGG 1 cut(s) 588
Bsc4I CCNNNNNNNGG 1 cut(s) 603
Bse1I ACTGG 1 cut(s) 640
Bse3DI GCAATG 1 cut(s) 167
BseBI CCWGG 1 cut(s) 414
BseDI CCNNGG 1 cut(s) 588
BseGI GGATG 3 cut(s) 612, 630, 640
BseLI CCNNNNNNNGG 1 cut(s) 603
BseMI GCAATG 1 cut(s) 167
BseNI ACTGG 1 cut(s) 640
BseRI GAGGAG 1 cut(s) 446
BseX3I CGGCCG 1 cut(s) 116
BseXI GCAGC 3 cut(s) 56, 439, 615
BsgI GTGCAG 1 cut(s) 438
Bsh1285I CGRYCG 1 cut(s) 119
BshFI GGCC 3 cut(s) 118, 356, 639
BsiEI CGRYCG 1 cut(s) 119
BsiHKAI GWGCWC 1 cut(s) 45
BslI CCNNNNNNNGG 1 cut(s) 603
BsmAI GTCTC 2 cut(s) 113, 559
BsnI GGCC 3 cut(s) 118, 356, 639
Bso31I GGTCTC 1 cut(s) 113
Bsp1286I GDGCHC 1 cut(s) 45
Bsp143I GATC 4 cut(s) 169, 409, 517, 615
Bsp1720I GCTNAGC 1 cut(s) 653
BspACI CCGC 3 cut(s) 115, 316, 597
BspANI GGCC 3 cut(s) 118, 356, 639
BspHI TCATGA 1 cut(s) 472
BspMAI CTGCAG 1 cut(s) 633
BspPI GGATC 1 cut(s) 404
BspTNI GGTCTC 1 cut(s) 113
BsrBI CCGCTC 1 cut(s) 316
BsrDI GCAATG 1 cut(s) 167
BsrI ACTGG 1 cut(s) 640
BssECI CCNNGG 1 cut(s) 588
BssMI GATC 4 cut(s) 169, 409, 517, 615
BssSI CACGAG 1 cut(s) 110
Bst2BI CACGAG 1 cut(s) 110
Bst2UI CCWGG 1 cut(s) 414
Bst4CI ACNGT 1 cut(s) 333
Bst6I CTCTTC 1 cut(s) 474
BstBAI YACGTR 1 cut(s) 56
BstC8I GCNNGC 1 cut(s) 445
BstDEI CTNAG 1 cut(s) 653
BstF5I GGATG 3 cut(s) 612, 630, 640
BstHHI GCGC 1 cut(s) 69
BstKTI GATC 4 cut(s) 172, 412, 520, 618
BstMAI GTCTC 2 cut(s) 113, 559
BstMBI GATC 4 cut(s) 169, 409, 517, 615
BstMCI CGRYCG 1 cut(s) 119
BstMWI GCNNNNNNNGC 3 cut(s) 15, 449, 461
BstNI CCWGG 1 cut(s) 414
BstSCI CCNGG 1 cut(s) 412
BstSFI CTRYAG 1 cut(s) 629
BstV1I GCAGC 3 cut(s) 56, 439, 615
BstX2I RGATCY 1 cut(s) 169
BstYI RGATCY 1 cut(s) 169
BstZI CGGCCG 1 cut(s) 116
BsuRI GGCC 3 cut(s) 118, 356, 639
BtsCI GGATG 3 cut(s) 612, 630, 640
BtsIMutI CAGTG 1 cut(s) 338
Cac8I GCNNGC 1 cut(s) 445
CciI TCATGA 1 cut(s) 472
CfoI GCGC 1 cut(s) 69
Cfr13I GGNCC 1 cut(s) 355
CviAII CATG 3 cut(s) 473, 613, 649
CviJI RGCY 8 cut(s) 101, 118, 289, 319, 356, 452, 639, 657
CviKI_1 RGCY 8 cut(s) 101, 118, 289, 319, 356, 452, 639, 657
DdeI CTNAG 1 cut(s) 653
DpnI GATC 4 cut(s) 171, 411, 519, 617
DpnII GATC 4 cut(s) 169, 409, 517, 615
EaeI YGGCCR 2 cut(s) 116, 637
EagI CGGCCG 1 cut(s) 116
Eam1104I CTCTTC 1 cut(s) 474
EarI CTCTTC 1 cut(s) 474
EclXI CGGCCG 1 cut(s) 116
Eco31I GGTCTC 1 cut(s) 113
Eco52I CGGCCG 1 cut(s) 116
EcoRII CCWGG 1 cut(s) 412
FaeI CATG 3 cut(s) 476, 616, 652
FaiI YATR 9 cut(s) 74, 154, 156, 339, 474, 533, 567, 614, 650
FalI AAGNNNNNCTT 2 cut(s) 453, 485
FatI CATG 3 cut(s) 472, 612, 648
FauNDI CATATG 1 cut(s) 154
FbaI TGATCA 1 cut(s) 615
Fnu4HI GCNGC 5 cut(s) 70, 116, 317, 453, 629
FokI GGATG 3 cut(s) 619, 627, 637
Fsp4HI GCNGC 5 cut(s) 70, 116, 317, 453, 629
GlaI GCGC 1 cut(s) 68
GluI GCNGC 5 cut(s) 70, 116, 317, 453, 629
HaeIII GGCC 3 cut(s) 118, 356, 639
HhaI GCGC 1 cut(s) 69
Hin1II CATG 3 cut(s) 476, 616, 652
Hin6I GCGC 1 cut(s) 67
HinP1I GCGC 1 cut(s) 67
HinfI GANTC 2 cut(s) 538, 593
Hpy166II GTNNAC 1 cut(s) 300
Hpy188I TCNGA 2 cut(s) 430, 592
Hpy188III TCNNGA 4 cut(s) 173, 473, 542, 619
Hpy8I GTNNAC 1 cut(s) 300
HpyCH4III ACNGT 1 cut(s) 333
HpyCH4IV ACGT 2 cut(s) 55, 522
HpyCH4V TGCA 5 cut(s) 72, 160, 425, 455, 631
HpyF10VI GCNNNNNNNGC 3 cut(s) 15, 449, 461
HpyF3I CTNAG 1 cut(s) 653
HpySE526I ACGT 2 cut(s) 55, 522
Hsp92II CATG 3 cut(s) 476, 616, 652
HspAI GCGC 1 cut(s) 67
Ksp22I TGATCA 1 cut(s) 615
Kzo9I GATC 4 cut(s) 169, 409, 517, 615
LpnPI CCDG 5 cut(s) 158, 236, 399, 426, 621
Lsp1109I GCAGC 3 cut(s) 56, 439, 615
LweI GCATC 2 cut(s) 434, 615
MaeII ACGT 2 cut(s) 55, 522
MalI GATC 4 cut(s) 171, 411, 519, 617
MbiI CCGCTC 1 cut(s) 316
MboI GATC 4 cut(s) 169, 409, 517, 615
MboII GAAGA 2 cut(s) 461, 523
MfeI CAATTG 1 cut(s) 84
MflI RGATCY 1 cut(s) 169
MhlI GDGCHC 1 cut(s) 45
MlsI TGGCCA 1 cut(s) 639
MluCI AATT 5 cut(s) 29, 84, 209, 343, 497
MluNI TGGCCA 1 cut(s) 639
MlyI GAGTC 1 cut(s) 587
MnlI CCTC 5 cut(s) 424, 429, 538, 598, 615
Mox20I TGGCCA 1 cut(s) 639
MscI TGGCCA 1 cut(s) 639
MslI CAYNNNNRTG 1 cut(s) 86
Msp20I TGGCCA 1 cut(s) 639
MspR9I CCNGG 1 cut(s) 414
MunI CAATTG 1 cut(s) 84
MvaI CCWGG 1 cut(s) 414
MwoI GCNNNNNNNGC 3 cut(s) 15, 449, 461
NdeI CATATG 1 cut(s) 154
NdeII GATC 4 cut(s) 169, 409, 517, 615
NlaIII CATG 3 cut(s) 476, 616, 652
OliI CACNNNNGTG 1 cut(s) 86
PagI TCATGA 1 cut(s) 472
PfeI GAWTC 1 cut(s) 538
PfoI TCCNGGA 1 cut(s) 412
PkrI GCNGC 5 cut(s) 71, 117, 318, 454, 630
PleI GAGTC 1 cut(s) 587
PpsI GAGTC 1 cut(s) 587
Ppu21I YACGTR 1 cut(s) 56
Psp6I CCWGG 1 cut(s) 412
PspGI CCWGG 1 cut(s) 412
PspPI GGNCC 1 cut(s) 355
PstI CTGCAG 1 cut(s) 633
PsuI RGATCY 1 cut(s) 169
RseI CAYNNNNRTG 1 cut(s) 86
SatI GCNGC 5 cut(s) 70, 116, 317, 453, 629
Sau3AI GATC 4 cut(s) 169, 409, 517, 615
Sau96I GGNCC 1 cut(s) 355
SchI GAGTC 1 cut(s) 587
ScrFI CCNGG 1 cut(s) 414
SduI GDGCHC 1 cut(s) 45
SetI ASST 7 cut(s) 58, 103, 109, 255, 454, 525, 659
SfaNI GCATC 2 cut(s) 434, 615
SfcI CTRYAG 1 cut(s) 629
SmiMI CAYNNNNRTG 1 cut(s) 86
SmlI CTYRAG 1 cut(s) 542
SmoI CTYRAG 1 cut(s) 542
Sse9I AATT 5 cut(s) 29, 84, 209, 343, 497
SsiI CCGC 3 cut(s) 115, 316, 597
StyD4I CCNGG 1 cut(s) 412
TaaI ACNGT 1 cut(s) 333
TaiI ACGT 2 cut(s) 58, 525
TaqI TCGA 2 cut(s) 39, 97
TasI AATT 5 cut(s) 29, 84, 209, 343, 497
TauI GCSGC 2 cut(s) 118, 319
TfiI GAWTC 1 cut(s) 538
TscAI CASTG 1 cut(s) 338
TseI GCWGC 3 cut(s) 69, 452, 628
TspDTI ATGAA 6 cut(s) 17, 202, 210, 461, 489, 582
TspRI CASTG 1 cut(s) 338
XapI RAATTY 1 cut(s) 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.