AT5G67540

Belongs to the glycosyl hydrolase 43 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004682
Physical Location & Seq
Reverse (-)
26944028 .. 26946377
2350 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G67540.2

Sequence Viewer

Length: 1401 bp
ATGTCGGGATATTCGTCTTCTGCTGGCCTCCGAGGATTTGCAGGAGGTTGCAGATATTCATTGCTTACTATTGTTTGGACCGTCGTCGGGTTTTTCCTGGTGGCTCATCTTATCTCTCTGTATAGCAGAAAAGATAACAACATCCATCAACAAGTTTCTTCAGATCAGTTACAAGTAGTACATCATCTTGCTCATCCTATTGTTCGTGAACTTATAAGAGTTGAAGAAGAGGTCCTGAGAATGCCGCCACCTAGGAAGCGTTCTCCTCGTACTAGTAAACGGAGATCAAGAAAACCGATCCCTCTCGTTGAGGAGTTTCTTGACGACAAGTCACCAATTCGACATCTTTTCTTTCCCGGTATCAAAACCGCCGCTTTTGGTCCCACCAAGGATATGGGAAACGAGACATCGTATTACTTCCCCGGGAAAATTTGGATGGACACACAAGGGAATCCAATTCAAGCACATGGCGGAGGGATTCTGCTTGACGTGAAATCTAACACTTACTATTGGTATGGAGAGTATAAAGATGGACCAACTTACCATGCTCACAAGAAAGGACCAGCTAGAGTTGATATTATTGGTGTTGGTTGTTACTCTTCAAAGGACTTATGGACATGGAAAAATGAAGGCATTGTACTCGGAGCTGAGGAAACAAACAAGACCCATGATTTGCATAAATCCAATGTTCTTGAACGACCCAAAGTGATCTACAATGAAAAGACAGAAAAGTATGTGATGTGGATGCATATCGACGATGCAAACTACACTAAAGCTTCAGTTGGTGTGGCCATTAGCAATAGCCCGACAGGTCCTTTCGAGTATCTATACAGTAAACGTCCACACGGGTTCGATAGTCGGGACATGACTGTCTTCAAAGACGACGATGGTGTTGCTTACCTGATATATTCGTCTGAAGTCAATAGTGTGCTTCATATTGGACCCTTGACGGAAGATTACCTCGATGTGACACCGGTTATGAAGAGAGTAATGGTGGGACAACACAGGGAAGCTCCAGCTATCTTCAAGCATCAGAATATTTATTACATGGTCACTTCCTGGTGCACTGGTTGGGCACCAAATGAAGCCTTGGCTCATGCGGCTGAATCTATAATGGGTCCATGGGAGAAATTGGGGAACCCGTGTATCGGTGGTAACAAAGTTTTCCGGTTAACAACATTCTTTGCGCAGAGCACTTATGTGATTCCTTTACCTGGTGTTCCCGGTGCGTTTATATTCATGGCAGATAGGTGGAACCCGGCTGATTTGCGGGATTCCAGATACGTGTGGTTACCGTTAGTTATAGGTGGACCTGCTGACCAGCCTTTGGAGTTCAACTTCGGGTTCCCATCGTGGTCCAGAGTGTCCATCTACTGGCACAGTAAATGGCGGCTGCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_43

Protein Analysis

466

Amino Acids

52.95

Weight (kDa)

8.85

Isoelectric Point (pI)

45.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_43 PF04616 199 - 382 5.1e-17 Glycosyl hydrolases family 43
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 215
AasI GACNNNNNNGTC 1 cut(s) 869
Acc16I TGCGCA 1 cut(s) 1188
Acc36I ACCTGC 1 cut(s) 1321
AccB1I GGYRCC 1 cut(s) 1075
AccB7I CCANNNNNTGG 2 cut(s) 1327, 1374
AciI CCGC 7 cut(s) 245, 369, 372, 471, 1100, 1270, 1390
AclWI GGATC 1 cut(s) 292
AcoI YGGCCR 1 cut(s) 789
AcsI RAATTY 1 cut(s) 429
AcuI CTGAAG 3 cut(s) 144, 762, 936
AfaI GTAC 3 cut(s) 180, 271, 639
AfiI CCNNNNNNNGG 5 cut(s) 87, 1148, 1214, 1327, 1374
AflIII ACRYGT 1 cut(s) 1284
AgeI ACCGGT 1 cut(s) 973
AgsI TTSAA 7 cut(s) 224, 461, 603, 695, 877, 1027, 1336
AhdI GACNNNNNGTC 1 cut(s) 328
AhlI ACTAGT 1 cut(s) 272
AjiI CACGTC 1 cut(s) 490
AjnI CCWGG 3 cut(s) 96, 1058, 1213
AleI CACNNNNGTG 1 cut(s) 1199
AluBI AGCT 5 cut(s) 566, 647, 776, 1013, 1019
AluI AGCT 5 cut(s) 566, 647, 776, 1013, 1019
Alw21I GWGCWC 2 cut(s) 1067, 1196
Alw26I GTCTC 1 cut(s) 398
Alw44I GTGCAC 1 cut(s) 1063
AlwI GGATC 1 cut(s) 292
Ama87I CYCGRG 1 cut(s) 422
AoxI GGCC 2 cut(s) 25, 789
ApaLI GTGCAC 1 cut(s) 1063
ApeKI GCWGC 1 cut(s) 1393
ApoI RAATTY 1 cut(s) 429
ArsI GACNNNNNNTTYG 2 cut(s) 655, 687
AsiGI ACCGGT 1 cut(s) 973
AspA2I CCTAGG 1 cut(s) 251
AspLEI GCGC 1 cut(s) 1189
AsuC2I CCSGG 5 cut(s) 357, 423, 424, 1224, 1259
AsuHPI GGTGA 1 cut(s) 324
AvaI CYCGRG 1 cut(s) 422
AvrII CCTAGG 1 cut(s) 251
BaeGI GKGCMC 2 cut(s) 1067, 1078
BaeI ACNNNNGTAYC 2 cut(s) 1129, 1162
BalI TGGCCA 1 cut(s) 791
BanI GGYRCC 1 cut(s) 1075
BarI GAAGNNNNNNTAC 2 cut(s) 621, 653
BbsI GAAGAC 2 cut(s) 9, 865
Bbv12I GWGCWC 2 cut(s) 1067, 1196
BbvCI CCTCAGC 1 cut(s) 648
BbvI GCAGC 1 cut(s) 1380
BccI CCATC 6 cut(s) 153, 430, 524, 881, 1357, 1376
BcgI CGANNNNNNTGC 4 cut(s) 622, 656, 875, 909
BciT130I CCWGG 3 cut(s) 98, 1060, 1215
BcnI CCSGG 5 cut(s) 357, 423, 424, 1224, 1259
BcoDI GTCTC 1 cut(s) 398
BcuI ACTAGT 1 cut(s) 272
BfaI CTAG 3 cut(s) 252, 273, 567
BfuAI ACCTGC 1 cut(s) 1321
BisI GCNGC 5 cut(s) 245, 372, 1101, 1391, 1394
BlnI CCTAGG 1 cut(s) 251
BlsI GCNGC 5 cut(s) 246, 373, 1102, 1392, 1395
Bme1390I CCNGG 8 cut(s) 98, 357, 423, 424, 1060, 1215, 1224, 1259
BmeRI GACNNNNNGTC 1 cut(s) 328
BmeT110I CYCGRG 1 cut(s) 422
BmgBI CACGTC 1 cut(s) 490
BmiI GGNNCC 7 cut(s) 382, 943, 1077, 1119, 1139, 1256, 1346
BmrFI CCNGG 8 cut(s) 98, 357, 423, 424, 1060, 1215, 1224, 1259
BmsI GCATC 3 cut(s) 735, 748, 1039
BoxI GACNNNNGTC 1 cut(s) 83
BpiI GAAGAC 2 cut(s) 9, 865
BpmI CTGGAG 1 cut(s) 999
Bpu10I CCTNAGC 1 cut(s) 648
BpuMI CCSGG 5 cut(s) 357, 423, 424, 1224, 1259
BsaAI YACGTR 1 cut(s) 1285
BsaBI GATNNNNATC 1 cut(s) 749
BsaJI CCNNGG 7 cut(s) 31, 251, 387, 421, 422, 1089, 1121
BsaWI WCCGGW 2 cut(s) 973, 1167
Bsc4I CCNNNNNNNGG 5 cut(s) 87, 1148, 1214, 1327, 1374
Bse118I RCCGGY 1 cut(s) 973
Bse1I ACTGG 2 cut(s) 1072, 1379
Bse3DI GCAATG 1 cut(s) 59
Bse8I GATNNNNATC 1 cut(s) 749
BseBI CCWGG 3 cut(s) 98, 1060, 1215
BseDI CCNNGG 7 cut(s) 31, 251, 387, 421, 422, 1089, 1121
BseGI GGATG 4 cut(s) 141, 193, 441, 750
BseJI GATNNNNATC 1 cut(s) 749
BseLI CCNNNNNNNGG 5 cut(s) 87, 1148, 1214, 1327, 1374
BseMI GCAATG 1 cut(s) 59
BseMII CTCAG 2 cut(s) 227, 639
BseNI ACTGG 2 cut(s) 1072, 1379
BseRI GAGGAG 2 cut(s) 255, 326
BseSI GKGCMC 2 cut(s) 1067, 1078
BseXI GCAGC 1 cut(s) 1380
BshFI GGCC 2 cut(s) 27, 791
BshNI GGYRCC 1 cut(s) 1075
BshTI ACCGGT 1 cut(s) 973
BsiHKAI GWGCWC 2 cut(s) 1067, 1196
BsiHKCI CYCGRG 1 cut(s) 422
BsiSI CCGG 6 cut(s) 357, 423, 974, 1168, 1224, 1259
BslFI GGGAC 3 cut(s) 366, 875, 1011
BslI CCNNNNNNNGG 5 cut(s) 87, 1148, 1214, 1327, 1374
BsmAI GTCTC 1 cut(s) 398
BsmFI GGGAC 3 cut(s) 366, 875, 1011
BsmI GAATGC 1 cut(s) 246
BsnI GGCC 2 cut(s) 27, 791
BsoBI CYCGRG 1 cut(s) 422
Bsp1286I GDGCHC 3 cut(s) 1067, 1078, 1196
Bsp143I GATC 4 cut(s) 163, 284, 297, 708
Bsp19I CCATGG 1 cut(s) 1121
BspACI CCGC 7 cut(s) 245, 369, 372, 471, 1100, 1270, 1390
BspANI GGCC 2 cut(s) 27, 791
BspCNI CTCAG 2 cut(s) 228, 640
BspLI GGNNCC 7 cut(s) 382, 943, 1077, 1119, 1139, 1256, 1346
BspMI ACCTGC 1 cut(s) 1321
BspPI GGATC 1 cut(s) 292
BspT107I GGYRCC 1 cut(s) 1075
BsrDI GCAATG 1 cut(s) 59
BsrFI RCCGGY 1 cut(s) 973
BsrI ACTGG 2 cut(s) 1072, 1379
BssAI RCCGGY 1 cut(s) 973
BssECI CCNNGG 7 cut(s) 31, 251, 387, 421, 422, 1089, 1121
BssMI GATC 4 cut(s) 163, 284, 297, 708
BssT1I CCWWGG 4 cut(s) 251, 387, 1089, 1121
Bst2UI CCWGG 3 cut(s) 98, 1060, 1215
Bst4CI ACNGT 5 cut(s) 82, 833, 871, 1296, 1382
Bst6I CTCTTC 3 cut(s) 222, 604, 977
BstBAI YACGTR 1 cut(s) 1285
BstC8I GCNNGC 1 cut(s) 25
BstDEI CTNAG 2 cut(s) 236, 648
BstDSI CCRYGG 1 cut(s) 1121
BstEII GGTNACC 1 cut(s) 1290
BstF5I GGATG 4 cut(s) 141, 193, 441, 750
BstHHI GCGC 1 cut(s) 1189
BstKTI GATC 4 cut(s) 166, 287, 300, 711
BstMAI GTCTC 1 cut(s) 398
BstMBI GATC 4 cut(s) 163, 284, 297, 708
BstMWI GCNNNNNNNGC 1 cut(s) 1100
BstNI CCWGG 3 cut(s) 98, 1060, 1215
BstPAI GACNNNNGTC 1 cut(s) 83
BstPI GGTNACC 1 cut(s) 1290
BstSCI CCNGG 8 cut(s) 96, 355, 421, 422, 1058, 1213, 1222, 1257
BstSLI GKGCMC 2 cut(s) 1067, 1078
BstV1I GCAGC 1 cut(s) 1380
BstV2I GAAGAC 2 cut(s) 9, 865
BstXI CCANNNNNNTGG 1 cut(s) 394
BsuRI GGCC 2 cut(s) 27, 791
BtgI CCRYGG 1 cut(s) 1121
BtrI CACGTC 1 cut(s) 490
BtsCI GGATG 4 cut(s) 141, 193, 441, 750
BtsIMutI CAGTG 1 cut(s) 1065
BveI ACCTGC 1 cut(s) 1321
Cac8I GCNNGC 1 cut(s) 25
CfoI GCGC 1 cut(s) 1189
Cfr10I RCCGGY 1 cut(s) 973
Cfr9I CCCGGG 1 cut(s) 422
CsiI ACCWGGT 1 cut(s) 1213
Csp6I GTAC 3 cut(s) 179, 270, 638
CspAI ACCGGT 1 cut(s) 973
CviAII CATG 9 cut(s) 467, 545, 618, 668, 865, 1048, 1097, 1122, 1240
CviQI GTAC 3 cut(s) 179, 270, 638
DdeI CTNAG 2 cut(s) 236, 648
DpnI GATC 4 cut(s) 165, 286, 299, 710
DpnII GATC 4 cut(s) 163, 284, 297, 708
DrdI GACNNNNNNGTC 1 cut(s) 869
DriI GACNNNNNGTC 1 cut(s) 328
DseDI GACNNNNNNGTC 1 cut(s) 869
EaeI YGGCCR 1 cut(s) 789
Eam1104I CTCTTC 3 cut(s) 222, 604, 977
Eam1105I GACNNNNNGTC 1 cut(s) 328
EarI CTCTTC 3 cut(s) 222, 604, 977
EciI GGCGGA 1 cut(s) 486
Eco130I CCWWGG 4 cut(s) 251, 387, 1089, 1121
Eco57I CTGAAG 3 cut(s) 144, 762, 936
Eco88I CYCGRG 1 cut(s) 422
Eco91I GGTNACC 1 cut(s) 1290
EcoO109I RGGNCCY 2 cut(s) 232, 812
EcoO65I GGTNACC 1 cut(s) 1290
EcoRII CCWGG 3 cut(s) 96, 1058, 1213
EcoT14I CCWWGG 4 cut(s) 251, 387, 1089, 1121
EcoT22I ATGCAT 1 cut(s) 750
ErhI CCWWGG 4 cut(s) 251, 387, 1089, 1121
FaeI CATG 9 cut(s) 470, 548, 621, 671, 868, 1051, 1100, 1125, 1243
FaqI GGGAC 3 cut(s) 366, 875, 1011
FatI CATG 9 cut(s) 466, 544, 617, 667, 864, 1047, 1096, 1121, 1239
FauI CCCGC 1 cut(s) 1263
Fnu4HI GCNGC 5 cut(s) 245, 372, 1101, 1391, 1394
FokI GGATG 4 cut(s) 128, 180, 448, 757
Fsp4HI GCNGC 5 cut(s) 245, 372, 1101, 1391, 1394
FspBI CTAG 3 cut(s) 252, 273, 567
FspI TGCGCA 1 cut(s) 1188
GlaI GCGC 1 cut(s) 1188
GluI GCNGC 5 cut(s) 245, 372, 1101, 1391, 1394
GsuI CTGGAG 1 cut(s) 999
HaeIII GGCC 2 cut(s) 27, 791
HapII CCGG 6 cut(s) 357, 423, 974, 1168, 1224, 1259
HhaI GCGC 1 cut(s) 1189
Hin1II CATG 9 cut(s) 470, 548, 621, 671, 868, 1051, 1100, 1125, 1243
Hin6I GCGC 1 cut(s) 1187
HinP1I GCGC 1 cut(s) 1187
HincII GTYRAC 1 cut(s) 1173
HindII GTYRAC 1 cut(s) 1173
HindIII AAGCTT 1 cut(s) 774
HinfI GANTC 5 cut(s) 451, 478, 1106, 1204, 1274
HpaI GTTAAC 1 cut(s) 1173
HpaII CCGG 6 cut(s) 357, 423, 974, 1168, 1224, 1259
HphI GGTGA 1 cut(s) 324
Hpy166II GTNNAC 7 cut(s) 209, 278, 836, 842, 1065, 1173, 1310
Hpy188I TCNGA 5 cut(s) 32, 163, 644, 916, 1035
Hpy188III TCNNGA 9 cut(s) 6, 206, 235, 288, 320, 692, 860, 1278, 1359
Hpy8I GTNNAC 7 cut(s) 209, 278, 836, 842, 1065, 1173, 1310
Hpy99I CGWCG 4 cut(s) 86, 89, 758, 887
HpyAV CCTTC 1 cut(s) 623
HpyCH4III ACNGT 5 cut(s) 82, 833, 871, 1296, 1382
HpyCH4IV ACGT 3 cut(s) 489, 838, 1284
HpyCH4V TGCA 6 cut(s) 41, 51, 676, 748, 761, 1065
HpyF10VI GCNNNNNNNGC 1 cut(s) 1100
HpyF3I CTNAG 2 cut(s) 236, 648
HpySE526I ACGT 3 cut(s) 489, 838, 1284
Hsp92II CATG 9 cut(s) 470, 548, 621, 671, 868, 1051, 1100, 1125, 1243
HspAI GCGC 1 cut(s) 1187
KspAI GTTAAC 1 cut(s) 1173
Kzo9I GATC 4 cut(s) 163, 284, 297, 708
LmnI GCTCC 2 cut(s) 644, 1018
Lsp1109I GCAGC 1 cut(s) 1380
LweI GCATC 3 cut(s) 735, 748, 1039
MabI ACCWGGT 1 cut(s) 1213
MaeI CTAG 3 cut(s) 252, 273, 567
MaeII ACGT 3 cut(s) 489, 838, 1284
MaeIII GTNAC 7 cut(s) 168, 330, 593, 967, 1051, 1154, 1290
MalI GATC 4 cut(s) 165, 286, 299, 710
MboI GATC 4 cut(s) 163, 284, 297, 708
MboII GAAGA 9 cut(s) 9, 150, 236, 239, 591, 865, 965, 994, 1015
MhlI GDGCHC 3 cut(s) 1067, 1078, 1196
MlsI TGGCCA 1 cut(s) 791
MluCI AATT 4 cut(s) 336, 429, 456, 1130
MluNI TGGCCA 1 cut(s) 791
Mox20I TGGCCA 1 cut(s) 791
Mph1103I ATGCAT 1 cut(s) 750
MscI TGGCCA 1 cut(s) 791
MseI TTAA 2 cut(s) 1172, 1399
MslI CAYNNNNRTG 1 cut(s) 1199
Msp20I TGGCCA 1 cut(s) 791
MspI CCGG 6 cut(s) 357, 423, 974, 1168, 1224, 1259
MspR9I CCNGG 8 cut(s) 98, 357, 423, 424, 1060, 1215, 1224, 1259
Mva1269I GAATGC 1 cut(s) 246
MvaI CCWGG 3 cut(s) 98, 1060, 1215
MwoI GCNNNNNNNGC 1 cut(s) 1100
NciI CCSGG 5 cut(s) 357, 423, 424, 1224, 1259
NcoI CCATGG 1 cut(s) 1121
NdeII GATC 4 cut(s) 163, 284, 297, 708
NlaIII CATG 9 cut(s) 470, 548, 621, 671, 868, 1051, 1100, 1125, 1243
NlaIV GGNNCC 7 cut(s) 382, 943, 1077, 1119, 1139, 1256, 1346
NmuCI GTSAC 3 cut(s) 330, 967, 1051
NsbI TGCGCA 1 cut(s) 1188
NsiI ATGCAT 1 cut(s) 750
OliI CACNNNNGTG 1 cut(s) 1199
PcsI WCGNNNNNNNCGW 1 cut(s) 11
PctI GAATGC 1 cut(s) 246
PfeI GAWTC 5 cut(s) 451, 478, 1106, 1204, 1274
PflMI CCANNNNNTGG 2 cut(s) 1327, 1374
PinAI ACCGGT 1 cut(s) 973
PkrI GCNGC 5 cut(s) 246, 373, 1102, 1392, 1395
Ppu21I YACGTR 1 cut(s) 1285
PpuMI RGGWCCY 2 cut(s) 232, 812
PshAI GACNNNNGTC 1 cut(s) 83
PsiI TTATAA 1 cut(s) 215
Psp5II RGGWCCY 2 cut(s) 232, 812
Psp6I CCWGG 3 cut(s) 96, 1058, 1213
PspEI GGTNACC 1 cut(s) 1290
PspGI CCWGG 3 cut(s) 96, 1058, 1213
PspN4I GGNNCC 7 cut(s) 382, 943, 1077, 1119, 1139, 1256, 1346
PspPPI RGGWCCY 2 cut(s) 232, 812
RsaI GTAC 3 cut(s) 180, 271, 639
RsaNI GTAC 3 cut(s) 179, 270, 638
RseI CAYNNNNRTG 1 cut(s) 1199
SaqAI TTAA 2 cut(s) 1172, 1399
SatI GCNGC 5 cut(s) 245, 372, 1101, 1391, 1394
Sau3AI GATC 4 cut(s) 163, 284, 297, 708
ScrFI CCNGG 8 cut(s) 98, 357, 423, 424, 1060, 1215, 1224, 1259
SduI GDGCHC 3 cut(s) 1067, 1078, 1196
SexAI ACCWGGT 1 cut(s) 1213
SfaNI GCATC 3 cut(s) 735, 748, 1039
SmaI CCCGGG 1 cut(s) 424
SmiMI CAYNNNNRTG 1 cut(s) 1199
SpeI ACTAGT 1 cut(s) 272
Sse9I AATT 4 cut(s) 336, 429, 456, 1130
SsiI CCGC 7 cut(s) 245, 369, 372, 471, 1100, 1270, 1390
SspI AATATT 1 cut(s) 1039
SspMI CTAG 3 cut(s) 252, 273, 567
StyD4I CCNGG 8 cut(s) 96, 355, 421, 422, 1058, 1213, 1222, 1257
StyI CCWWGG 4 cut(s) 251, 387, 1089, 1121
TaaI ACNGT 5 cut(s) 82, 833, 871, 1296, 1382
TaiI ACGT 3 cut(s) 492, 841, 1287
TaqI TCGA 5 cut(s) 340, 753, 819, 852, 963
TasI AATT 4 cut(s) 336, 429, 456, 1130
TatI WGTACW 2 cut(s) 178, 637
TauI GCSGC 4 cut(s) 247, 374, 1103, 1393
TfiI GAWTC 5 cut(s) 451, 478, 1106, 1204, 1274
Tru1I TTAA 2 cut(s) 1172, 1399
Tru9I TTAA 2 cut(s) 1172, 1399
TscAI CASTG 1 cut(s) 1072
TseFI GTSAC 3 cut(s) 330, 967, 1051
TseI GCWGC 1 cut(s) 1393
Tsp45I GTSAC 3 cut(s) 330, 967, 1051
TspDTI ATGAA 7 cut(s) 48, 642, 732, 923, 995, 1098, 1228
TspGWI ACGGA 2 cut(s) 295, 965
TspMI CCCGGG 1 cut(s) 422
TspRI CASTG 1 cut(s) 1072
Van91I CCANNNNNTGG 2 cut(s) 1327, 1374
VneI GTGCAC 1 cut(s) 1063
XapI RAATTY 1 cut(s) 429
XcmI CCANNNNNNNNNTGG 1 cut(s) 391
XmaI CCCGGG 1 cut(s) 422
XmaJI CCTAGG 1 cut(s) 251
XspI CTAG 3 cut(s) 252, 273, 567
Zsp2I ATGCAT 1 cut(s) 750
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.