MD02G1186100.v1.1

Belongs to the glycosyl hydrolase 43 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0004682
Physical Location & Seq
Reverse (-)
16904912 .. 16909402
4491 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1186100.v1.1.491

Sequence Viewer

Length: 1374 bp
ATGCTCCAGATGTTTTTAGGGAACATCTGTTCAACGTCTGTGGTGGTGTGGAGCTTGGTGGGATGTCTTCTTATGTTTCATCTGTACACTCTTGTTCAGCCAAAGGATAGGTTGGGAGGAGAAATTCAAGTCCGAGTGAGTCATTACCCACAGTTCCATGAACTTGAACAGGTGGAAGAGGAAAACATCCAAATTCCTCTGAAGAGAAAGCGACCCCCACATGCTGCAAAAAGAAAACTAAGGCGACCAACTCCTCTGATTGATGAATTTCTTGATGAGAATTCTCAAATTAGACACGTATTCTTTCCCCTGAAGCCTGATATTGATCCAATGAAGGACACAGGAAATGACAGCTACTACTATTTCCCTGGAAGGATTTGGTTGGATACTGATGGAAATCCTATTCAAGCCCATGGAGGTGGTATTTTATTCGACGAAAAATCAAGGACATACTATTGGTATGGAGAGTATAAAGATGGGCCCACATACCATGCTCACAAAAAAGGAGCAGCTCGGGTTGACATCATGGGAGTGGGTTGCTATTCTTCCAATGACTTATGGAAATGGAAAAATGAAGGTATTGTATTGGCAGCAGAAAAAGAAAATAAAACACATGATCTCCACGAATTCAATGTTCTTGAGAGGCCGAAAGTGATTTACAATGAGCATACAGGGAAGTACGTTATGTGGATGCACATTGATGATGCTAACTATACCAAAGCTTCTGTTGGCGTTGCCATTAGTGATTACCCAACTGGTCCTTTTGACTATCTCTACAGCCAACAACCCCATGGATTTGATAGTAGGGACATGACAATCTTCAAAGATGATGATGGTGTTGCTTATCTAATATACTCCTCGGACCACAATAATGAACTTCATATTGGACCATTGACTGAAGATTACCTTCACGTGACGAACATCATGAGAAGAATTTTTGTGGGACAACGTCGGGAAGCCCCAGCTCTATTCAAGCATGAAGGAACTTATTACATGATTACTTCAGGCTGCTCCGGATGGGCTCCTAATGAGGCACTGGCGCATGCAGCAGAGTCAATCATGGGGCCATGGGAGACCATGGGAACCCCCTGCGCTGGAGGGAACAAAGTGTCTCGACTTACTACATTTTTTGCACAGAGCACATTTGTGCTTCCTGTGCCGGGATTTCCTGGTTCATTTATCTTCATGGCAGATAGATGGAACCCGGCAGACTTGAGAGACTCAAGATATGTATGGTTGCCTTTGATAGTAGGGGGGACAGCTGACCGGCCCCTCGATTACAAATTCGGGTTCCCGCTGTGGTCAAGAGTGTCAATATATTGGCATAGAAAGTGGAAACTTCCTCAGGGGTGGAGCGGGTGGAAACGAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_43

Protein Analysis

458

Amino Acids

52.7

Weight (kDa)

6.68

Isoelectric Point (pI)

45.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_43 PF04616 176 - 359 3.1e-18 Glycosyl hydrolases family 43
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1356
AccIII TCCGGA 1 cut(s) 1013
AciI CCGC 2 cut(s) 1295, 1356
AclWI GGATC 1 cut(s) 320
AcsI RAATTY 7 cut(s) 123, 192, 266, 280, 626, 933, 1283
AcuI CTGAAG 4 cut(s) 221, 332, 918, 987
AcvI CACGTG 1 cut(s) 913
AfaI GTAC 2 cut(s) 86, 680
AfiI CCNNNNNNNGG 2 cut(s) 1094, 1160
AflIII ACRYGT 1 cut(s) 295
AgsI TTSAA 7 cut(s) 33, 128, 167, 407, 631, 823, 973
AjnI CCWGG 2 cut(s) 367, 1168
AjuI GAANNNNNNNTTGG 2 cut(s) 867, 899
AleI CACNNNNGTG 1 cut(s) 1145
AloI GAACNNNNNNTCC 2 cut(s) 13, 45
AluBI AGCT 6 cut(s) 54, 354, 512, 722, 965, 1262
AluI AGCT 6 cut(s) 54, 354, 512, 722, 965, 1262
Alw21I GWGCWC 1 cut(s) 1142
Alw26I GTCTC 3 cut(s) 1067, 1116, 1212
AlwI GGATC 1 cut(s) 320
Ama87I CYCGRG 1 cut(s) 513
Aor13HI TCCGGA 1 cut(s) 1013
AoxI GGCC 4 cut(s) 479, 644, 1064, 1268
ApaI GGGCCC 1 cut(s) 483
ApeKI GCWGC 5 cut(s) 224, 509, 590, 1008, 1046
ApoI RAATTY 7 cut(s) 123, 192, 266, 280, 626, 933, 1283
AspLEI GCGC 2 cut(s) 1042, 1094
AspS9I GGNCC 7 cut(s) 479, 480, 758, 862, 887, 1064, 1269
AsuC2I CCSGG 2 cut(s) 1161, 1205
AvaI CYCGRG 1 cut(s) 513
AvaII GGWCC 3 cut(s) 758, 862, 887
AxyI CCTNAGG 1 cut(s) 1344
BaeGI GKGCMC 1 cut(s) 483
BanII GRGCYC 2 cut(s) 483, 1024
BbrPI CACGTG 1 cut(s) 913
BbsI GAAGAC 1 cut(s) 59
Bbv12I GWGCWC 1 cut(s) 1142
BbvI GCAGC 5 cut(s) 211, 521, 602, 995, 1058
BccI CCATC 5 cut(s) 386, 470, 827, 1011, 1191
BciT130I CCWGG 2 cut(s) 369, 1170
BciVI GTATCC 1 cut(s) 379
BcnI CCSGG 2 cut(s) 1161, 1205
BcoDI GTCTC 3 cut(s) 1067, 1116, 1212
BfmI CTRYAG 1 cut(s) 775
BfuI GTATCC 1 cut(s) 379
BisI GCNGC 5 cut(s) 225, 510, 591, 1009, 1047
BlsI GCNGC 5 cut(s) 226, 511, 592, 1010, 1048
Bme1390I CCNGG 4 cut(s) 369, 1161, 1170, 1205
Bme18I GGWCC 3 cut(s) 758, 862, 887
BmeT110I CYCGRG 1 cut(s) 513
BmgT120I GGNCC 7 cut(s) 479, 480, 758, 862, 887, 1064, 1269
BmiI GGNNCC 7 cut(s) 481, 1023, 1065, 1084, 1202, 1271, 1292
BmrFI CCNGG 4 cut(s) 369, 1161, 1170, 1205
BmsI GCATC 2 cut(s) 681, 694
BpiI GAAGAC 1 cut(s) 59
BpmI CTGGAG 1 cut(s) 1116
BpuEI CTTGAG 3 cut(s) 659, 1207, 1234
BpuMI CCSGG 2 cut(s) 1161, 1205
BsaAI YACGTR 2 cut(s) 298, 913
BsaBI GATNNNNATC 2 cut(s) 324, 396
BsaI GGTCTC 1 cut(s) 1067
BsaJI CCNNGG 6 cut(s) 367, 412, 790, 858, 1067, 1077
BsaWI WCCGGW 1 cut(s) 1013
BsaXI ACNNNNNCTCC 2 cut(s) 603, 633
Bsc4I CCNNNNNNNGG 2 cut(s) 1094, 1160
Bse118I RCCGGY 1 cut(s) 1266
Bse1I ACTGG 2 cut(s) 760, 1041
Bse21I CCTNAGG 1 cut(s) 1344
Bse8I GATNNNNATC 2 cut(s) 324, 396
BseAI TCCGGA 1 cut(s) 1013
BseBI CCWGG 2 cut(s) 369, 1170
BseDI CCNNGG 6 cut(s) 367, 412, 790, 858, 1067, 1077
BseGI GGATG 4 cut(s) 68, 186, 696, 1022
BseJI GATNNNNATC 2 cut(s) 324, 396
BseLI CCNNNNNNNGG 2 cut(s) 1094, 1160
BseMII CTCAG 1 cut(s) 1358
BseNI ACTGG 2 cut(s) 760, 1041
BseRI GAGGAG 3 cut(s) 132, 243, 847
BseSI GKGCMC 1 cut(s) 483
BseXI GCAGC 5 cut(s) 211, 521, 602, 995, 1058
BseYI CCCAGC 1 cut(s) 961
BshFI GGCC 4 cut(s) 481, 646, 1066, 1270
BsiHKAI GWGCWC 1 cut(s) 1142
BsiHKCI CYCGRG 1 cut(s) 513
BsiSI CCGG 4 cut(s) 1014, 1160, 1205, 1267
BslFI GGGAC 3 cut(s) 821, 957, 1270
BslI CCNNNNNNNGG 2 cut(s) 1094, 1160
BsmAI GTCTC 3 cut(s) 1067, 1116, 1212
BsmFI GGGAC 3 cut(s) 821, 957, 1270
BsnI GGCC 4 cut(s) 481, 646, 1066, 1270
Bso31I GGTCTC 1 cut(s) 1067
BsoBI CYCGRG 1 cut(s) 513
Bsp120I GGGCCC 1 cut(s) 479
Bsp1286I GDGCHC 3 cut(s) 483, 1024, 1142
Bsp13I TCCGGA 1 cut(s) 1013
Bsp1407I TGTACA 1 cut(s) 84
Bsp143I GATC 2 cut(s) 325, 616
Bsp19I CCATGG 4 cut(s) 412, 790, 1067, 1077
BspACI CCGC 2 cut(s) 1295, 1356
BspANI GGCC 4 cut(s) 481, 646, 1066, 1270
BspCNI CTCAG 1 cut(s) 1357
BspEI TCCGGA 1 cut(s) 1013
BspHI TCATGA 1 cut(s) 924
BspLI GGNNCC 7 cut(s) 481, 1023, 1065, 1084, 1202, 1271, 1292
BspPI GGATC 1 cut(s) 320
BspTNI GGTCTC 1 cut(s) 1067
BsrBI CCGCTC 1 cut(s) 1356
BsrFI RCCGGY 1 cut(s) 1266
BsrGI TGTACA 1 cut(s) 84
BsrI ACTGG 2 cut(s) 760, 1041
BssAI RCCGGY 1 cut(s) 1266
BssECI CCNNGG 6 cut(s) 367, 412, 790, 858, 1067, 1077
BssMI GATC 2 cut(s) 325, 616
BssT1I CCWWGG 4 cut(s) 412, 790, 1067, 1077
Bst2UI CCWGG 2 cut(s) 369, 1170
Bst4CI ACNGT 1 cut(s) 153
Bst6I CTCTTC 2 cut(s) 171, 197
BstAUI TGTACA 1 cut(s) 84
BstBAI YACGTR 2 cut(s) 298, 913
BstC8I GCNNGC 1 cut(s) 1044
BstDEI CTNAG 2 cut(s) 239, 1344
BstDSI CCRYGG 4 cut(s) 412, 790, 1067, 1077
BstF5I GGATG 4 cut(s) 68, 186, 696, 1022
BstHHI GCGC 2 cut(s) 1042, 1094
BstKTI GATC 2 cut(s) 328, 619
BstMAI GTCTC 3 cut(s) 1067, 1116, 1212
BstMBI GATC 2 cut(s) 325, 616
BstMWI GCNNNNNNNGC 2 cut(s) 1046, 1156
BstNI CCWGG 2 cut(s) 369, 1170
BstNSI RCATGY 2 cut(s) 224, 1046
BstSCI CCNGG 4 cut(s) 367, 1159, 1168, 1203
BstSFI CTRYAG 1 cut(s) 775
BstSLI GKGCMC 1 cut(s) 483
BstV1I GCAGC 5 cut(s) 211, 521, 602, 995, 1058
BstV2I GAAGAC 1 cut(s) 59
BstXI CCANNNNNNTGG 1 cut(s) 419
Bsu36I CCTNAGG 1 cut(s) 1344
BsuI GTATCC 1 cut(s) 379
BsuRI GGCC 4 cut(s) 481, 646, 1066, 1270
BtgI CCRYGG 4 cut(s) 412, 790, 1067, 1077
BtsCI GGATG 4 cut(s) 68, 186, 696, 1022
BtsIMutI CAGTG 1 cut(s) 1034
Cac8I GCNNGC 1 cut(s) 1044
CciI TCATGA 1 cut(s) 924
CfoI GCGC 2 cut(s) 1042, 1094
Cfr10I RCCGGY 1 cut(s) 1266
Cfr13I GGNCC 7 cut(s) 479, 480, 758, 862, 887, 1064, 1269
Csp6I GTAC 2 cut(s) 85, 679
CspCI CAANNNNNGTGG 2 cut(s) 21, 56
CviQI GTAC 2 cut(s) 85, 679
DdeI CTNAG 2 cut(s) 239, 1344
DpnI GATC 2 cut(s) 327, 618
DpnII GATC 2 cut(s) 325, 616
Eam1104I CTCTTC 2 cut(s) 171, 197
EarI CTCTTC 2 cut(s) 171, 197
Eco130I CCWWGG 4 cut(s) 412, 790, 1067, 1077
Eco24I GRGCYC 2 cut(s) 483, 1024
Eco31I GGTCTC 1 cut(s) 1067
Eco47I GGWCC 3 cut(s) 758, 862, 887
Eco57I CTGAAG 4 cut(s) 221, 332, 918, 987
Eco72I CACGTG 1 cut(s) 913
Eco81I CCTNAGG 1 cut(s) 1344
Eco88I CYCGRG 1 cut(s) 513
EcoRI GAATTC 2 cut(s) 280, 626
EcoRII CCWGG 2 cut(s) 367, 1168
EcoT14I CCWWGG 4 cut(s) 412, 790, 1067, 1077
EcoT38I GRGCYC 2 cut(s) 483, 1024
ErhI CCWWGG 4 cut(s) 412, 790, 1067, 1077
FalI AAGNNNNNCTT 2 cut(s) 891, 923
FaqI GGGAC 3 cut(s) 821, 957, 1270
FauI CCCGC 2 cut(s) 1302, 1349
Fnu4HI GCNGC 5 cut(s) 225, 510, 591, 1009, 1047
FokI GGATG 4 cut(s) 75, 173, 703, 1029
FriOI GRGCYC 2 cut(s) 483, 1024
Fsp4HI GCNGC 5 cut(s) 225, 510, 591, 1009, 1047
GlaI GCGC 2 cut(s) 1041, 1093
GluI GCNGC 5 cut(s) 225, 510, 591, 1009, 1047
GsaI CCCAGC 1 cut(s) 965
GsuI CTGGAG 1 cut(s) 1116
HaeIII GGCC 4 cut(s) 481, 646, 1066, 1270
HapII CCGG 4 cut(s) 1014, 1160, 1205, 1267
HhaI GCGC 2 cut(s) 1042, 1094
Hin6I GCGC 2 cut(s) 1040, 1092
HinP1I GCGC 2 cut(s) 1040, 1092
HincII GTYRAC 1 cut(s) 520
HindII GTYRAC 1 cut(s) 520
HindIII AAGCTT 1 cut(s) 720
HinfI GANTC 3 cut(s) 139, 1052, 1220
HpaII CCGG 4 cut(s) 1014, 1160, 1205, 1267
Hpy166II GTNNAC 2 cut(s) 87, 520
Hpy188I TCNGA 4 cut(s) 134, 201, 258, 862
Hpy188III TCNNGA 9 cut(s) 7, 272, 638, 925, 953, 1014, 1113, 1224, 1305
Hpy8I GTNNAC 2 cut(s) 87, 520
Hpy99I CGWCG 2 cut(s) 437, 954
HpyAV CCTTC 5 cut(s) 328, 366, 569, 917, 974
HpyCH4III ACNGT 1 cut(s) 153
HpyCH4IV ACGT 5 cut(s) 35, 297, 681, 912, 949
HpyCH4V TGCA 4 cut(s) 227, 694, 1046, 1133
HpyF10VI GCNNNNNNNGC 2 cut(s) 1046, 1156
HpyF3I CTNAG 2 cut(s) 239, 1344
HpySE526I ACGT 5 cut(s) 35, 297, 681, 912, 949
HspAI GCGC 2 cut(s) 1040, 1092
Kpn2I TCCGGA 1 cut(s) 1013
Kzo9I GATC 2 cut(s) 325, 616
LmnI GCTCC 6 cut(s) 9, 51, 506, 1016, 1027, 1353
Lsp1109I GCAGC 5 cut(s) 211, 521, 602, 995, 1058
LweI GCATC 2 cut(s) 681, 694
MaeII ACGT 5 cut(s) 35, 297, 681, 912, 949
MaeIII GTNAC 1 cut(s) 913
MalI GATC 2 cut(s) 327, 618
MbiI CCGCTC 1 cut(s) 1356
MboI GATC 2 cut(s) 325, 616
MboII GAAGA 8 cut(s) 59, 188, 214, 537, 811, 911, 942, 1174
MhlI GDGCHC 3 cut(s) 483, 1024, 1142
MluCI AATT 8 cut(s) 123, 192, 266, 280, 288, 626, 933, 1283
MlyI GAGTC 3 cut(s) 148, 1061, 1214
MmeI TCCRAC 1 cut(s) 363
MroI TCCGGA 1 cut(s) 1013
MslI CAYNNNNRTG 5 cut(s) 417, 530, 699, 870, 1145
MspA1I CMGCKG 2 cut(s) 1262, 1297
MspI CCGG 4 cut(s) 1014, 1160, 1205, 1267
MspR9I CCNGG 4 cut(s) 369, 1161, 1170, 1205
MvaI CCWGG 2 cut(s) 369, 1170
MwoI GCNNNNNNNGC 2 cut(s) 1046, 1156
NciI CCSGG 2 cut(s) 1161, 1205
NcoI CCATGG 4 cut(s) 412, 790, 1067, 1077
NdeII GATC 2 cut(s) 325, 616
NlaIV GGNNCC 7 cut(s) 481, 1023, 1065, 1084, 1202, 1271, 1292
NmuCI GTSAC 1 cut(s) 913
NspI RCATGY 2 cut(s) 224, 1046
OliI CACNNNNGTG 1 cut(s) 1145
PaeI GCATGC 1 cut(s) 1046
PagI TCATGA 1 cut(s) 924
PflFI GACNNNGTC 1 cut(s) 948
PkrI GCNGC 5 cut(s) 226, 511, 592, 1010, 1048
PleI GAGTC 3 cut(s) 147, 1060, 1214
PmaCI CACGTG 1 cut(s) 913
PmlI CACGTG 1 cut(s) 913
PpsI GAGTC 3 cut(s) 147, 1060, 1214
Ppu21I YACGTR 2 cut(s) 298, 913
Psp6I CCWGG 2 cut(s) 367, 1168
PspCI CACGTG 1 cut(s) 913
PspFI CCCAGC 1 cut(s) 961
PspGI CCWGG 2 cut(s) 367, 1168
PspN4I GGNNCC 7 cut(s) 481, 1023, 1065, 1084, 1202, 1271, 1292
PspOMI GGGCCC 1 cut(s) 479
PspPI GGNCC 7 cut(s) 479, 480, 758, 862, 887, 1064, 1269
PsyI GACNNNGTC 1 cut(s) 948
PvuII CAGCTG 1 cut(s) 1262
RsaI GTAC 2 cut(s) 86, 680
RsaNI GTAC 2 cut(s) 85, 679
RseI CAYNNNNRTG 5 cut(s) 417, 530, 699, 870, 1145
SatI GCNGC 5 cut(s) 225, 510, 591, 1009, 1047
Sau3AI GATC 2 cut(s) 325, 616
Sau96I GGNCC 7 cut(s) 479, 480, 758, 862, 887, 1064, 1269
SchI GAGTC 3 cut(s) 148, 1061, 1214
ScrFI CCNGG 4 cut(s) 369, 1161, 1170, 1205
SduI GDGCHC 3 cut(s) 483, 1024, 1142
SfaNI GCATC 2 cut(s) 681, 694
SfcI CTRYAG 1 cut(s) 775
SinI GGWCC 3 cut(s) 758, 862, 887
SmiMI CAYNNNNRTG 5 cut(s) 417, 530, 699, 870, 1145
SmlI CTYRAG 3 cut(s) 638, 1213, 1222
SmoI CTYRAG 3 cut(s) 638, 1213, 1222
SphI GCATGC 1 cut(s) 1046
Sse9I AATT 8 cut(s) 123, 192, 266, 280, 288, 626, 933, 1283
SsiI CCGC 2 cut(s) 1295, 1356
StyD4I CCNGG 4 cut(s) 367, 1159, 1168, 1203
StyI CCWWGG 4 cut(s) 412, 790, 1067, 1077
TaaI ACNGT 1 cut(s) 153
TaiI ACGT 5 cut(s) 38, 300, 684, 915, 952
TaqI TCGA 3 cut(s) 432, 1114, 1275
TasI AATT 8 cut(s) 123, 192, 266, 280, 288, 626, 933, 1283
TatI WGTACW 1 cut(s) 84
TscAI CASTG 1 cut(s) 1041
TseFI GTSAC 1 cut(s) 913
TseI GCWGC 5 cut(s) 224, 509, 590, 1008, 1046
Tsp45I GTSAC 1 cut(s) 913
TspRI CASTG 1 cut(s) 1041
Tth111I GACNNNGTC 1 cut(s) 948
VpaK11BI GGWCC 3 cut(s) 758, 862, 887
XapI RAATTY 7 cut(s) 123, 192, 266, 280, 626, 933, 1283
XceI RCATGY 2 cut(s) 224, 1046
XcmI CCANNNNNNNNNTGG 1 cut(s) 788
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.