pycom02g15120

Belongs to the glycosyl hydrolase 43 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0004682
Physical Location & Seq
Reverse (-)
11825725 .. 11829986
4262 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g15120.1

Sequence Viewer

Length: 1452 bp
ATGCTCAGATGTTTTCAGCATTATCTTGGAGAGAATAAAAAGGATGAGAAAATGACGATCAGGAACAAATACAGGAAACCAACCACTTTCCGTTGCAATGCAGGGAGCAGATGTTCAACGTCTGCTGTGGTGTGGAGCTTGGTGGGATGTCTTCTTATGTTTCATCTGTACACTCTTGTTCGGCAAAAGGATAGGTTAGGAGGAGCAATTCAATTTCGAGCGAGTCATCACCCACTGTTCCAGGAACTTGAACAGGTGGAAGAGGAAAACATCCAACTTCCTCCGCCGCGAAAGCGATCCCCACGTGCTGAAAAAAGAAAACCAAGGCGACCAACTACTCTGATTGATGAATTTCTTGATGAGAATTCTCAAATTAGACACGTATTCTTTCCCCAGAAGCTTGATATTGATCCAATGAAGGACACAGGAAATGATAGCTATTACTATTACCCTGGGAGGATTTGGTTGGATACTGATGGATATCCTATTCAAGCCCATGGAGGTGGTATTCTATTCGATGAAAAATCAAGGACATACTATTGGTACGGGGAGTATAAAGATGGCCCCACATACCATGCTCACAAAAAAGGAGCAGCTCGGGTTGACATCATAGGAGTGGGTTGCTATTCTTCCAACGACTTATGGAAATGGAAAAATGAAGGTGTTGTATTGGCAGCAGAAAAAGCAAATGAAACACATGATCTCCACGAATCCAATGTGCTTGAGAGGCCGAAAGTGATTTACAATGAGCATACAGGGAAGTACGTTATGTGGATGCACATTGATGATGCTAACTATACTAAAGCTTCTGTTGGCGTTGCCATTAGTGATTACCCAACCGGTCCCTTTGATTATCTCTACAGCCAACAACCCCATGGATTTGATAGTAGGGACATGACAATCTTCAAAGATGATGATGGTATTGCTTATCTAATATACTCCTCCGAGGACAATAGTGAACTTCATATTGGACCACTGACTGAAGATTACCTTGACGTGACAAATACCGTGAGAAGAATTCTTGTGGGACAACATCGGGAAGCCCCGGCTCTATTCAAGCATGAAGGAACTTATTACATGATTACTTCAGGCTGCACCGGATGGGCTCCTAATGAGGCACTGGTGCATGCAGCAGAATCAATCAAGGGGCCATGGGAGACCATGGGAAACCCCTGTGCTGGAGGGAACAAAGTGTCTCGACTTGCTACATTTTTTGCACAGAGCACATTTGTGCTTCCTGTGCCTGGATTTCCTGGTTCATTTATCTTCATGGCAGATAGATGGAACCCGGCGGACTTGAGAGACTCAAGATATGTATGGTTGCCTTTGATAGTAGGGGGGCCAGCTGACCGGCCCCTCAATTACAATTTTGGGTTCCCGCTATGGTCAAGAGTGTCAATATATTGGCATAGAAAATGGAAACTTCCTCAGGGGTGGAGCGGGTCGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_43

Protein Analysis

484

Amino Acids

55.55

Weight (kDa)

7.66

Isoelectric Point (pI)

44.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_43 PF04616 204 - 388 1e-19 Glycosyl hydrolases family 43
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1440
AccII CGCG 1 cut(s) 289
AciI CCGC 5 cut(s) 284, 287, 1292, 1379, 1440
AclWI GGATC 2 cut(s) 291, 404
AcsI RAATTY 3 cut(s) 350, 364, 1017
AcuI CTGAAG 2 cut(s) 1002, 1071
AcvI CACGTG 1 cut(s) 305
AfaI GTAC 3 cut(s) 170, 545, 764
AfiI CCNNNNNNNGG 2 cut(s) 1178, 1244
AflIII ACRYGT 1 cut(s) 379
AgeI ACCGGT 1 cut(s) 839
AgsI TTSAA 6 cut(s) 117, 212, 251, 491, 907, 1057
AjiI CACGTC 1 cut(s) 997
AjnI CCWGG 4 cut(s) 240, 451, 1243, 1252
AjuI GAANNNNNNNTTGG 2 cut(s) 951, 983
AleI CACNNNNGTG 1 cut(s) 1229
AloI GAACNNNNNNTCC 2 cut(s) 97, 129
AluBI AGCT 6 cut(s) 138, 400, 438, 596, 806, 1346
AluI AGCT 6 cut(s) 138, 400, 438, 596, 806, 1346
Alw21I GWGCWC 1 cut(s) 1226
Alw26I GTCTC 3 cut(s) 1151, 1200, 1296
AlwI GGATC 2 cut(s) 291, 404
Ama87I CYCGRG 1 cut(s) 597
AoxI GGCC 5 cut(s) 562, 728, 1148, 1340, 1352
ApeKI GCWGC 4 cut(s) 593, 674, 1092, 1130
ApoI RAATTY 3 cut(s) 350, 364, 1017
AsiGI ACCGGT 1 cut(s) 839
AspS9I GGNCC 6 cut(s) 563, 842, 971, 1148, 1340, 1353
AsuC2I CCSGG 2 cut(s) 1046, 1289
AsuHPI GGTGA 1 cut(s) 221
AvaI CYCGRG 1 cut(s) 597
AvaII GGWCC 2 cut(s) 842, 971
AxyI CCTNAGG 1 cut(s) 1428
BanII GRGCYC 1 cut(s) 1108
BbrPI CACGTG 1 cut(s) 305
BbsI GAAGAC 1 cut(s) 143
Bbv12I GWGCWC 1 cut(s) 1226
BbvI GCAGC 4 cut(s) 605, 686, 1079, 1142
BccI CCATC 5 cut(s) 470, 554, 911, 1095, 1275
BciT130I CCWGG 4 cut(s) 242, 453, 1245, 1254
BciVI GTATCC 1 cut(s) 463
BcnI CCSGG 2 cut(s) 1046, 1289
BcoDI GTCTC 3 cut(s) 1151, 1200, 1296
BfmI CTRYAG 1 cut(s) 859
BfuI GTATCC 1 cut(s) 463
BisI GCNGC 5 cut(s) 287, 594, 675, 1093, 1131
BlsI GCNGC 5 cut(s) 288, 595, 676, 1094, 1132
Bme1390I CCNGG 6 cut(s) 242, 453, 1046, 1245, 1254, 1289
Bme18I GGWCC 2 cut(s) 842, 971
BmeT110I CYCGRG 1 cut(s) 597
BmgBI CACGTC 1 cut(s) 997
BmgT120I GGNCC 6 cut(s) 563, 842, 971, 1148, 1340, 1353
BmiI GGNNCC 8 cut(s) 565, 844, 1107, 1149, 1286, 1341, 1355, 1376
BmrFI CCNGG 6 cut(s) 242, 453, 1046, 1245, 1254, 1289
BmsI GCATC 2 cut(s) 765, 778
BpiI GAAGAC 1 cut(s) 143
BpmI CTGGAG 1 cut(s) 1200
BpuEI CTTGAG 3 cut(s) 743, 1291, 1318
BpuMI CCSGG 2 cut(s) 1046, 1289
BsaAI YACGTR 2 cut(s) 305, 382
BsaBI GATNNNNATC 2 cut(s) 408, 480
BsaI GGTCTC 1 cut(s) 1151
BsaJI CCNNGG 9 cut(s) 323, 451, 452, 496, 874, 945, 1044, 1151, 1161
BsaWI WCCGGW 2 cut(s) 839, 1097
BsaXI ACNNNNNCTCC 4 cut(s) 97, 127, 687, 717
Bsc4I CCNNNNNNNGG 2 cut(s) 1178, 1244
Bse118I RCCGGY 2 cut(s) 839, 1350
Bse1I ACTGG 1 cut(s) 1125
Bse21I CCTNAGG 1 cut(s) 1428
Bse3DI GCAATG 1 cut(s) 103
Bse8I GATNNNNATC 2 cut(s) 408, 480
BseBI CCWGG 4 cut(s) 242, 453, 1245, 1254
BseDI CCNNGG 9 cut(s) 323, 451, 452, 496, 874, 945, 1044, 1151, 1161
BseGI GGATG 5 cut(s) 49, 152, 270, 780, 1106
BseJI GATNNNNATC 2 cut(s) 408, 480
BseLI CCNNNNNNNGG 2 cut(s) 1178, 1244
BseMI GCAATG 1 cut(s) 103
BseMII CTCAG 2 cut(s) 19, 1442
BseNI ACTGG 1 cut(s) 1125
BseRI GAGGAG 2 cut(s) 216, 931
BseXI GCAGC 4 cut(s) 605, 686, 1079, 1142
BsgI GTGCAG 1 cut(s) 1078
Bsh1236I CGCG 1 cut(s) 289
BshFI GGCC 5 cut(s) 564, 730, 1150, 1342, 1354
BshTI ACCGGT 1 cut(s) 839
BsiHKAI GWGCWC 1 cut(s) 1226
BsiHKCI CYCGRG 1 cut(s) 597
BsiSI CCGG 5 cut(s) 840, 1046, 1098, 1289, 1351
BslFI GGGAC 3 cut(s) 828, 905, 1041
BslI CCNNNNNNNGG 2 cut(s) 1178, 1244
BsmAI GTCTC 3 cut(s) 1151, 1200, 1296
BsmFI GGGAC 3 cut(s) 828, 905, 1041
BsnI GGCC 5 cut(s) 564, 730, 1150, 1342, 1354
Bso31I GGTCTC 1 cut(s) 1151
BsoBI CYCGRG 1 cut(s) 597
Bsp1286I GDGCHC 2 cut(s) 1108, 1226
Bsp1407I TGTACA 1 cut(s) 168
Bsp143I GATC 4 cut(s) 57, 296, 409, 700
Bsp19I CCATGG 4 cut(s) 496, 874, 1151, 1161
BspACI CCGC 5 cut(s) 284, 287, 1292, 1379, 1440
BspANI GGCC 5 cut(s) 564, 730, 1150, 1342, 1354
BspCNI CTCAG 2 cut(s) 18, 1441
BspFNI CGCG 1 cut(s) 289
BspLI GGNNCC 8 cut(s) 565, 844, 1107, 1149, 1286, 1341, 1355, 1376
BspPI GGATC 2 cut(s) 291, 404
BspTNI GGTCTC 1 cut(s) 1151
BsrBI CCGCTC 1 cut(s) 1440
BsrDI GCAATG 1 cut(s) 103
BsrFI RCCGGY 2 cut(s) 839, 1350
BsrGI TGTACA 1 cut(s) 168
BsrI ACTGG 1 cut(s) 1125
BssAI RCCGGY 2 cut(s) 839, 1350
BssECI CCNNGG 9 cut(s) 323, 451, 452, 496, 874, 945, 1044, 1151, 1161
BssMI GATC 4 cut(s) 57, 296, 409, 700
BssT1I CCWWGG 5 cut(s) 323, 496, 874, 1151, 1161
Bst2UI CCWGG 4 cut(s) 242, 453, 1245, 1254
Bst4CI ACNGT 2 cut(s) 237, 1009
Bst6I CTCTTC 1 cut(s) 255
BstAUI TGTACA 1 cut(s) 168
BstBAI YACGTR 2 cut(s) 305, 382
BstC8I GCNNGC 2 cut(s) 1128, 1344
BstDEI CTNAG 2 cut(s) 5, 1428
BstDSI CCRYGG 4 cut(s) 496, 874, 1151, 1161
BstF5I GGATG 5 cut(s) 49, 152, 270, 780, 1106
BstFNI CGCG 1 cut(s) 289
BstKTI GATC 4 cut(s) 60, 299, 412, 703
BstMAI GTCTC 3 cut(s) 1151, 1200, 1296
BstMBI GATC 4 cut(s) 57, 296, 409, 700
BstMWI GCNNNNNNNGC 4 cut(s) 292, 683, 727, 1240
BstNI CCWGG 4 cut(s) 242, 453, 1245, 1254
BstNSI RCATGY 1 cut(s) 1130
BstSCI CCNGG 6 cut(s) 240, 451, 1044, 1243, 1252, 1287
BstSFI CTRYAG 1 cut(s) 859
BstUI CGCG 1 cut(s) 289
BstV1I GCAGC 4 cut(s) 605, 686, 1079, 1142
BstV2I GAAGAC 1 cut(s) 143
BstXI CCANNNNNNTGG 1 cut(s) 503
Bsu36I CCTNAGG 1 cut(s) 1428
BsuI GTATCC 1 cut(s) 463
BsuRI GGCC 5 cut(s) 564, 730, 1150, 1342, 1354
BtgI CCRYGG 4 cut(s) 496, 874, 1151, 1161
BtrI CACGTC 1 cut(s) 997
BtsCI GGATG 5 cut(s) 49, 152, 270, 780, 1106
BtsIMutI CAGTG 3 cut(s) 233, 974, 1118
Cac8I GCNNGC 2 cut(s) 1128, 1344
Cfr10I RCCGGY 2 cut(s) 839, 1350
Cfr13I GGNCC 6 cut(s) 563, 842, 971, 1148, 1340, 1353
Csp6I GTAC 3 cut(s) 169, 544, 763
CspAI ACCGGT 1 cut(s) 839
CviQI GTAC 3 cut(s) 169, 544, 763
DdeI CTNAG 2 cut(s) 5, 1428
DpnI GATC 4 cut(s) 59, 298, 411, 702
DpnII GATC 4 cut(s) 57, 296, 409, 700
Eam1104I CTCTTC 1 cut(s) 255
EarI CTCTTC 1 cut(s) 255
EciI GGCGGA 2 cut(s) 273, 1307
Eco130I CCWWGG 5 cut(s) 323, 496, 874, 1151, 1161
Eco24I GRGCYC 1 cut(s) 1108
Eco31I GGTCTC 1 cut(s) 1151
Eco32I GATATC 1 cut(s) 482
Eco47I GGWCC 2 cut(s) 842, 971
Eco57I CTGAAG 2 cut(s) 1002, 1071
Eco72I CACGTG 1 cut(s) 305
Eco81I CCTNAGG 1 cut(s) 1428
Eco88I CYCGRG 1 cut(s) 597
EcoRI GAATTC 2 cut(s) 364, 1017
EcoRII CCWGG 4 cut(s) 240, 451, 1243, 1252
EcoRV GATATC 1 cut(s) 482
EcoT14I CCWWGG 5 cut(s) 323, 496, 874, 1151, 1161
EcoT38I GRGCYC 1 cut(s) 1108
ErhI CCWWGG 5 cut(s) 323, 496, 874, 1151, 1161
FalI AAGNNNNNCTT 2 cut(s) 975, 1007
FaqI GGGAC 3 cut(s) 828, 905, 1041
FauI CCCGC 2 cut(s) 1386, 1433
Fnu4HI GCNGC 5 cut(s) 287, 594, 675, 1093, 1131
FokI GGATG 5 cut(s) 56, 159, 257, 787, 1113
FriOI GRGCYC 1 cut(s) 1108
Fsp4HI GCNGC 5 cut(s) 287, 594, 675, 1093, 1131
GluI GCNGC 5 cut(s) 287, 594, 675, 1093, 1131
GsuI CTGGAG 1 cut(s) 1200
HaeIII GGCC 5 cut(s) 564, 730, 1150, 1342, 1354
HapII CCGG 5 cut(s) 840, 1046, 1098, 1289, 1351
HincII GTYRAC 1 cut(s) 604
HindII GTYRAC 1 cut(s) 604
HindIII AAGCTT 2 cut(s) 398, 804
HinfI GANTC 4 cut(s) 223, 710, 1136, 1304
HpaII CCGG 5 cut(s) 840, 1046, 1098, 1289, 1351
HphI GGTGA 1 cut(s) 221
Hpy166II GTNNAC 3 cut(s) 171, 604, 959
Hpy188I TCNGA 3 cut(s) 8, 342, 946
Hpy188III TCNNGA 6 cut(s) 61, 356, 1037, 1197, 1308, 1389
Hpy8I GTNNAC 3 cut(s) 171, 604, 959
HpyAV CCTTC 3 cut(s) 412, 653, 1058
HpyCH4III ACNGT 2 cut(s) 237, 1009
HpyCH4IV ACGT 5 cut(s) 119, 304, 381, 765, 996
HpyCH4V TGCA 7 cut(s) 96, 101, 778, 1095, 1126, 1130, 1217
HpyF10VI GCNNNNNNNGC 4 cut(s) 292, 683, 727, 1240
HpyF3I CTNAG 2 cut(s) 5, 1428
HpySE526I ACGT 5 cut(s) 119, 304, 381, 765, 996
Kzo9I GATC 4 cut(s) 57, 296, 409, 700
LmnI GCTCC 6 cut(s) 105, 135, 203, 590, 1111, 1437
Lsp1109I GCAGC 4 cut(s) 605, 686, 1079, 1142
LweI GCATC 2 cut(s) 765, 778
MaeII ACGT 5 cut(s) 119, 304, 381, 765, 996
MaeIII GTNAC 1 cut(s) 997
MalI GATC 4 cut(s) 59, 298, 411, 702
MbiI CCGCTC 1 cut(s) 1440
MboI GATC 4 cut(s) 57, 296, 409, 700
MboII GAAGA 7 cut(s) 143, 272, 621, 895, 995, 1026, 1258
MhlI GDGCHC 2 cut(s) 1108, 1226
MluCI AATT 8 cut(s) 207, 212, 350, 364, 372, 1017, 1360, 1366
MlyI GAGTC 2 cut(s) 232, 1298
MmeI TCCRAC 3 cut(s) 298, 447, 657
MslI CAYNNNNRTG 4 cut(s) 501, 614, 783, 1229
MspA1I CMGCKG 1 cut(s) 1346
MspI CCGG 5 cut(s) 840, 1046, 1098, 1289, 1351
MspR9I CCNGG 6 cut(s) 242, 453, 1046, 1245, 1254, 1289
MvaI CCWGG 4 cut(s) 242, 453, 1245, 1254
MvnI CGCG 1 cut(s) 289
MwoI GCNNNNNNNGC 4 cut(s) 292, 683, 727, 1240
NciI CCSGG 2 cut(s) 1046, 1289
NcoI CCATGG 4 cut(s) 496, 874, 1151, 1161
NdeII GATC 4 cut(s) 57, 296, 409, 700
NlaIV GGNNCC 8 cut(s) 565, 844, 1107, 1149, 1286, 1341, 1355, 1376
NmuCI GTSAC 1 cut(s) 997
NspI RCATGY 1 cut(s) 1130
OliI CACNNNNGTG 1 cut(s) 1229
PaeI GCATGC 1 cut(s) 1130
PasI CCCWGGG 1 cut(s) 452
PfeI GAWTC 2 cut(s) 710, 1136
PfoI TCCNGGA 1 cut(s) 240
PinAI ACCGGT 1 cut(s) 839
PkrI GCNGC 5 cut(s) 288, 595, 676, 1094, 1132
PleI GAGTC 2 cut(s) 231, 1298
PmaCI CACGTG 1 cut(s) 305
PmlI CACGTG 1 cut(s) 305
PpsI GAGTC 2 cut(s) 231, 1298
Ppu21I YACGTR 2 cut(s) 305, 382
Psp6I CCWGG 4 cut(s) 240, 451, 1243, 1252
PspCI CACGTG 1 cut(s) 305
PspGI CCWGG 4 cut(s) 240, 451, 1243, 1252
PspN4I GGNNCC 8 cut(s) 565, 844, 1107, 1149, 1286, 1341, 1355, 1376
PspPI GGNCC 6 cut(s) 563, 842, 971, 1148, 1340, 1353
PvuII CAGCTG 1 cut(s) 1346
RsaI GTAC 3 cut(s) 170, 545, 764
RsaNI GTAC 3 cut(s) 169, 544, 763
RseI CAYNNNNRTG 4 cut(s) 501, 614, 783, 1229
SatI GCNGC 5 cut(s) 287, 594, 675, 1093, 1131
Sau3AI GATC 4 cut(s) 57, 296, 409, 700
Sau96I GGNCC 6 cut(s) 563, 842, 971, 1148, 1340, 1353
SchI GAGTC 2 cut(s) 232, 1298
ScrFI CCNGG 6 cut(s) 242, 453, 1046, 1245, 1254, 1289
SduI GDGCHC 2 cut(s) 1108, 1226
SfaNI GCATC 2 cut(s) 765, 778
SfcI CTRYAG 1 cut(s) 859
SinI GGWCC 2 cut(s) 842, 971
SmiMI CAYNNNNRTG 4 cut(s) 501, 614, 783, 1229
SmlI CTYRAG 3 cut(s) 722, 1297, 1306
SmoI CTYRAG 3 cut(s) 722, 1297, 1306
SphI GCATGC 1 cut(s) 1130
Sse9I AATT 8 cut(s) 207, 212, 350, 364, 372, 1017, 1360, 1366
SsiI CCGC 5 cut(s) 284, 287, 1292, 1379, 1440
StyD4I CCNGG 6 cut(s) 240, 451, 1044, 1243, 1252, 1287
StyI CCWWGG 5 cut(s) 323, 496, 874, 1151, 1161
TaaI ACNGT 2 cut(s) 237, 1009
TaiI ACGT 5 cut(s) 122, 307, 384, 768, 999
TaqI TCGA 4 cut(s) 217, 516, 1198, 1445
TasI AATT 8 cut(s) 207, 212, 350, 364, 372, 1017, 1360, 1366
TatI WGTACW 1 cut(s) 168
TauI GCSGC 1 cut(s) 289
TfiI GAWTC 2 cut(s) 710, 1136
TscAI CASTG 3 cut(s) 240, 981, 1125
TseFI GTSAC 1 cut(s) 997
TseI GCWGC 4 cut(s) 593, 674, 1092, 1130
Tsp45I GTSAC 1 cut(s) 997
TspGWI ACGGA 1 cut(s) 80
TspRI CASTG 3 cut(s) 240, 981, 1125
VpaK11BI GGWCC 2 cut(s) 842, 971
XapI RAATTY 3 cut(s) 350, 364, 1017
XceI RCATGY 1 cut(s) 1130
XcmI CCANNNNNNNNNTGG 1 cut(s) 872
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.