MD02G1186200.v1.1

Belongs to the glycosyl hydrolase 43 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0004682
Physical Location & Seq
Reverse (-)
16929676 .. 16934250
4575 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1186200.v1.1.491

Sequence Viewer

Length: 1407 bp
ATGAGGATCAGGAACAAATACAGGAAACCAACCACTTTCCGTTGCAATGCAGGGAGCAGATGTTCAACGTCTGCTGTGGTGTGGAGCTTGGTGGGATGTCTTCTTATGTTTCATCTGTACACTCTTGTTCGGCAAAAGGATAGGTTAGGAGGAGCAATTCAATTTCGAGCGAGTCATCACCCACTGTTCCAGGAACTTGAACAGGTGGAAGAGGAAAACATCCAACTTCCTCCACCGCGAAAGCGATCCCCACGTGCTGAAAAAAGAAAACCAAGGCGACCAACTACTCTGATTGATGAATTTCTTGATGAGAATTCTCAAATTAGACACGTATTCTTTCCCCAGAAGCTTGATATTGATCCAATGAAGGACACAGGAAATGATAGCTATTACTATTACCCTGGGAGGATTTGGTTGGATACTGATGGATATCCTATTCAAGCCCATGGAGGTGGTATTCTATTCAATGAAAAATCAAGGACATACTATTGGTACGGGGAGTATAAAGATGGGCCCACATACCATGCTCACAAAAAAGGAGCAGCTCGGGTTGACATCATAGGAGTGGGTTGCTATTCTTCCAACGACTTATGGAAATGGAAAAATGAAGGTGTTGTATTGGCTGCAGAAAAAGCAAATGAAACTCATGATCTCCATGAATCCAATGTGCTTGAGAGGCCGAAAGTGATTTACAATGAGCATACAGGGAAGTATGTTATGTGGATGCACATTGATGATGCTAACTATACCAAAGCTTCTGTTGGCGTTGCCATTAGTGATTACCCAACCGGTCCTTTTGATTATCTCTACAGCCAACGACCCCATGGATTTGAAAGTAGGGACATGACAATCTTCAAAGATGATGATGGTGTTGCTTATCTAATATACTCCTCCGAGGACAATAGTGAACTTCATATTGGACCACTGACTGAAGATTACCTTGACGTGACAAATACTGTGAGAAGAATTCTTGTGGGACAACATCGGGAAGCCCCGGCTCTATTCAAGCATGAAGGAACTTATTACATGATTACTTCAGGCTGCACTGGATGGGCTCCTAATGAGGCACTGGTGCATGCAGCAGAATCAATCATGGGGCCATGGGAGACCATGGGGAACCCCTGCGCTGGAGGGAACAAAGTGTCTCGACTTGCTACATTTTTTGCACAGAGCACATTTGTGCTTCCTGTGCCTGGATTTCCTGGTGCATTTATCTTCATGGCAGATAGATGGAACCCGGCGGACTTGAGAGACTCAAGATATGTATGGTTGCCTTTGATAGTAGGGGGGCCAGCTGACCGGCCCTTCGATTACAATTTTGGGTTCCCGTTATGGTCAAGAGTGTCAATATATTGGCATAGAAAATGGAAACTTCCTCAGGGGTGGAGCGGGTCGAAACGAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_43

Protein Analysis

469

Amino Acids

53.82

Weight (kDa)

8.32

Isoelectric Point (pI)

44.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_43 PF04616 187 - 371 5.1e-20 Glycosyl hydrolases family 43
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1389
AccII CGCG 1 cut(s) 238
AciI CCGC 3 cut(s) 236, 1241, 1389
AclWI GGATC 3 cut(s) 14, 240, 353
AcsI RAATTY 3 cut(s) 299, 313, 966
AcuI CTGAAG 2 cut(s) 951, 1020
AcvI CACGTG 1 cut(s) 254
AfaI GTAC 2 cut(s) 119, 494
AfiI CCNNNNNNNGG 2 cut(s) 1127, 1193
AflIII ACRYGT 1 cut(s) 328
AgeI ACCGGT 1 cut(s) 788
AgsI TTSAA 8 cut(s) 66, 161, 200, 440, 466, 833, 856, 1006
AjiI CACGTC 1 cut(s) 946
AjnI CCWGG 4 cut(s) 189, 400, 1192, 1201
AjuI GAANNNNNNNTTGG 2 cut(s) 900, 932
AleI CACNNNNGTG 1 cut(s) 1178
AloI GAACNNNNNNTCC 2 cut(s) 46, 78
AluBI AGCT 6 cut(s) 87, 349, 387, 545, 755, 1295
AluI AGCT 6 cut(s) 87, 349, 387, 545, 755, 1295
Alw21I GWGCWC 1 cut(s) 1175
Alw26I GTCTC 3 cut(s) 1100, 1149, 1245
AlwI GGATC 3 cut(s) 14, 240, 353
Ama87I CYCGRG 1 cut(s) 546
AoxI GGCC 5 cut(s) 512, 677, 1097, 1289, 1301
ApaI GGGCCC 1 cut(s) 516
ApeKI GCWGC 4 cut(s) 542, 623, 1041, 1079
ApoI RAATTY 3 cut(s) 299, 313, 966
ArsI GACNNNNNNTTYG 2 cut(s) 1289, 1321
AsiGI ACCGGT 1 cut(s) 788
AspLEI GCGC 1 cut(s) 1127
AspS9I GGNCC 7 cut(s) 512, 513, 791, 920, 1097, 1289, 1302
AsuC2I CCSGG 2 cut(s) 995, 1238
AsuHPI GGTGA 1 cut(s) 170
AvaI CYCGRG 1 cut(s) 546
AvaII GGWCC 2 cut(s) 791, 920
AxyI CCTNAGG 1 cut(s) 1377
BaeGI GKGCMC 1 cut(s) 516
BanII GRGCYC 2 cut(s) 516, 1057
BbrPI CACGTG 1 cut(s) 254
BbsI GAAGAC 1 cut(s) 92
Bbv12I GWGCWC 1 cut(s) 1175
BbvI GCAGC 4 cut(s) 554, 610, 1028, 1091
BccI CCATC 5 cut(s) 419, 503, 860, 1044, 1224
BciT130I CCWGG 4 cut(s) 191, 402, 1194, 1203
BciVI GTATCC 1 cut(s) 412
BcnI CCSGG 2 cut(s) 995, 1238
BcoDI GTCTC 3 cut(s) 1100, 1149, 1245
BfmI CTRYAG 2 cut(s) 624, 808
BfuI GTATCC 1 cut(s) 412
BisI GCNGC 4 cut(s) 543, 624, 1042, 1080
BlsI GCNGC 4 cut(s) 544, 625, 1043, 1081
Bme1390I CCNGG 6 cut(s) 191, 402, 995, 1194, 1203, 1238
Bme18I GGWCC 2 cut(s) 791, 920
BmeT110I CYCGRG 1 cut(s) 546
BmgBI CACGTC 1 cut(s) 946
BmgT120I GGNCC 7 cut(s) 512, 513, 791, 920, 1097, 1289, 1302
BmiI GGNNCC 7 cut(s) 514, 1056, 1098, 1118, 1235, 1290, 1325
BmrFI CCNGG 6 cut(s) 191, 402, 995, 1194, 1203, 1238
BmsI GCATC 2 cut(s) 714, 727
BpiI GAAGAC 1 cut(s) 92
BpmI CTGGAG 1 cut(s) 1149
BpuEI CTTGAG 3 cut(s) 692, 1240, 1267
BpuMI CCSGG 2 cut(s) 995, 1238
BsaAI YACGTR 2 cut(s) 254, 331
BsaBI GATNNNNATC 2 cut(s) 357, 429
BsaI GGTCTC 1 cut(s) 1100
BsaJI CCNNGG 9 cut(s) 272, 400, 401, 445, 823, 894, 993, 1100, 1110
BsaWI WCCGGW 1 cut(s) 788
BsaXI ACNNNNNCTCC 2 cut(s) 46, 76
Bsc4I CCNNNNNNNGG 2 cut(s) 1127, 1193
Bse118I RCCGGY 2 cut(s) 788, 1299
Bse1I ACTGG 2 cut(s) 1051, 1074
Bse21I CCTNAGG 1 cut(s) 1377
Bse3DI GCAATG 1 cut(s) 52
Bse8I GATNNNNATC 2 cut(s) 357, 429
BseBI CCWGG 4 cut(s) 191, 402, 1194, 1203
BseDI CCNNGG 9 cut(s) 272, 400, 401, 445, 823, 894, 993, 1100, 1110
BseGI GGATG 4 cut(s) 101, 219, 729, 1055
BseJI GATNNNNATC 2 cut(s) 357, 429
BseLI CCNNNNNNNGG 2 cut(s) 1127, 1193
BseMI GCAATG 1 cut(s) 52
BseMII CTCAG 1 cut(s) 1391
BseNI ACTGG 2 cut(s) 1051, 1074
BseRI GAGGAG 2 cut(s) 165, 880
BseSI GKGCMC 1 cut(s) 516
BseXI GCAGC 4 cut(s) 554, 610, 1028, 1091
BsgI GTGCAG 1 cut(s) 1027
Bsh1236I CGCG 1 cut(s) 238
BshFI GGCC 5 cut(s) 514, 679, 1099, 1291, 1303
BshTI ACCGGT 1 cut(s) 788
BsiHKAI GWGCWC 1 cut(s) 1175
BsiHKCI CYCGRG 1 cut(s) 546
BsiSI CCGG 4 cut(s) 789, 995, 1238, 1300
BslFI GGGAC 2 cut(s) 854, 990
BslI CCNNNNNNNGG 2 cut(s) 1127, 1193
BsmAI GTCTC 3 cut(s) 1100, 1149, 1245
BsmFI GGGAC 2 cut(s) 854, 990
BsnI GGCC 5 cut(s) 514, 679, 1099, 1291, 1303
Bso31I GGTCTC 1 cut(s) 1100
BsoBI CYCGRG 1 cut(s) 546
Bsp120I GGGCCC 1 cut(s) 512
Bsp1286I GDGCHC 3 cut(s) 516, 1057, 1175
Bsp1407I TGTACA 1 cut(s) 117
Bsp143I GATC 4 cut(s) 6, 245, 358, 649
Bsp19I CCATGG 4 cut(s) 445, 823, 1100, 1110
BspACI CCGC 3 cut(s) 236, 1241, 1389
BspANI GGCC 5 cut(s) 514, 679, 1099, 1291, 1303
BspCNI CTCAG 1 cut(s) 1390
BspFNI CGCG 1 cut(s) 238
BspHI TCATGA 1 cut(s) 646
BspLI GGNNCC 7 cut(s) 514, 1056, 1098, 1118, 1235, 1290, 1325
BspMAI CTGCAG 1 cut(s) 628
BspPI GGATC 3 cut(s) 14, 240, 353
BspTNI GGTCTC 1 cut(s) 1100
BsrBI CCGCTC 1 cut(s) 1389
BsrDI GCAATG 1 cut(s) 52
BsrFI RCCGGY 2 cut(s) 788, 1299
BsrGI TGTACA 1 cut(s) 117
BsrI ACTGG 2 cut(s) 1051, 1074
BssAI RCCGGY 2 cut(s) 788, 1299
BssECI CCNNGG 9 cut(s) 272, 400, 401, 445, 823, 894, 993, 1100, 1110
BssMI GATC 4 cut(s) 6, 245, 358, 649
BssT1I CCWWGG 5 cut(s) 272, 445, 823, 1100, 1110
Bst2UI CCWGG 4 cut(s) 191, 402, 1194, 1203
Bst4CI ACNGT 2 cut(s) 186, 958
Bst6I CTCTTC 1 cut(s) 204
BstAUI TGTACA 1 cut(s) 117
BstBAI YACGTR 2 cut(s) 254, 331
BstC8I GCNNGC 2 cut(s) 1077, 1293
BstDEI CTNAG 1 cut(s) 1377
BstDSI CCRYGG 4 cut(s) 445, 823, 1100, 1110
BstF5I GGATG 4 cut(s) 101, 219, 729, 1055
BstFNI CGCG 1 cut(s) 238
BstHHI GCGC 1 cut(s) 1127
BstKTI GATC 4 cut(s) 9, 248, 361, 652
BstMAI GTCTC 3 cut(s) 1100, 1149, 1245
BstMBI GATC 4 cut(s) 6, 245, 358, 649
BstMWI GCNNNNNNNGC 3 cut(s) 632, 676, 1189
BstNI CCWGG 4 cut(s) 191, 402, 1194, 1203
BstNSI RCATGY 1 cut(s) 1079
BstSCI CCNGG 6 cut(s) 189, 400, 993, 1192, 1201, 1236
BstSFI CTRYAG 2 cut(s) 624, 808
BstSLI GKGCMC 1 cut(s) 516
BstUI CGCG 1 cut(s) 238
BstV1I GCAGC 4 cut(s) 554, 610, 1028, 1091
BstV2I GAAGAC 1 cut(s) 92
BstXI CCANNNNNNTGG 1 cut(s) 452
Bsu36I CCTNAGG 1 cut(s) 1377
BsuI GTATCC 1 cut(s) 412
BsuRI GGCC 5 cut(s) 514, 679, 1099, 1291, 1303
BtgI CCRYGG 4 cut(s) 445, 823, 1100, 1110
BtrI CACGTC 1 cut(s) 946
BtsCI GGATG 4 cut(s) 101, 219, 729, 1055
BtsIMutI CAGTG 4 cut(s) 182, 923, 1044, 1067
Cac8I GCNNGC 2 cut(s) 1077, 1293
CciI TCATGA 1 cut(s) 646
CfoI GCGC 1 cut(s) 1127
Cfr10I RCCGGY 2 cut(s) 788, 1299
Cfr13I GGNCC 7 cut(s) 512, 513, 791, 920, 1097, 1289, 1302
Csp6I GTAC 2 cut(s) 118, 493
CspAI ACCGGT 1 cut(s) 788
CviQI GTAC 2 cut(s) 118, 493
DdeI CTNAG 1 cut(s) 1377
DpnI GATC 4 cut(s) 8, 247, 360, 651
DpnII GATC 4 cut(s) 6, 245, 358, 649
Eam1104I CTCTTC 1 cut(s) 204
EarI CTCTTC 1 cut(s) 204
EciI GGCGGA 1 cut(s) 1256
Eco130I CCWWGG 5 cut(s) 272, 445, 823, 1100, 1110
Eco24I GRGCYC 2 cut(s) 516, 1057
Eco31I GGTCTC 1 cut(s) 1100
Eco32I GATATC 1 cut(s) 431
Eco47I GGWCC 2 cut(s) 791, 920
Eco57I CTGAAG 2 cut(s) 951, 1020
Eco72I CACGTG 1 cut(s) 254
Eco81I CCTNAGG 1 cut(s) 1377
Eco88I CYCGRG 1 cut(s) 546
EcoRI GAATTC 2 cut(s) 313, 966
EcoRII CCWGG 4 cut(s) 189, 400, 1192, 1201
EcoRV GATATC 1 cut(s) 431
EcoT14I CCWWGG 5 cut(s) 272, 445, 823, 1100, 1110
EcoT38I GRGCYC 2 cut(s) 516, 1057
ErhI CCWWGG 5 cut(s) 272, 445, 823, 1100, 1110
FalI AAGNNNNNCTT 2 cut(s) 924, 956
FaqI GGGAC 2 cut(s) 854, 990
FauI CCCGC 1 cut(s) 1382
Fnu4HI GCNGC 4 cut(s) 543, 624, 1042, 1080
FokI GGATG 4 cut(s) 108, 206, 736, 1062
FriOI GRGCYC 2 cut(s) 516, 1057
Fsp4HI GCNGC 4 cut(s) 543, 624, 1042, 1080
GlaI GCGC 1 cut(s) 1126
GluI GCNGC 4 cut(s) 543, 624, 1042, 1080
GsuI CTGGAG 1 cut(s) 1149
HaeIII GGCC 5 cut(s) 514, 679, 1099, 1291, 1303
HapII CCGG 4 cut(s) 789, 995, 1238, 1300
HhaI GCGC 1 cut(s) 1127
Hin6I GCGC 1 cut(s) 1125
HinP1I GCGC 1 cut(s) 1125
HincII GTYRAC 1 cut(s) 553
HindII GTYRAC 1 cut(s) 553
HindIII AAGCTT 2 cut(s) 347, 753
HinfI GANTC 4 cut(s) 172, 659, 1085, 1253
HpaII CCGG 4 cut(s) 789, 995, 1238, 1300
HphI GGTGA 1 cut(s) 170
Hpy166II GTNNAC 3 cut(s) 120, 553, 908
Hpy188I TCNGA 2 cut(s) 291, 895
Hpy188III TCNNGA 7 cut(s) 10, 305, 647, 986, 1146, 1257, 1338
Hpy8I GTNNAC 3 cut(s) 120, 553, 908
HpyAV CCTTC 4 cut(s) 361, 602, 1007, 1315
HpyCH4III ACNGT 2 cut(s) 186, 958
HpyCH4IV ACGT 4 cut(s) 68, 253, 330, 945
HpyCH4V TGCA 9 cut(s) 45, 50, 626, 727, 1044, 1075, 1079, 1166, 1208
HpyF10VI GCNNNNNNNGC 3 cut(s) 632, 676, 1189
HpyF3I CTNAG 1 cut(s) 1377
HpySE526I ACGT 4 cut(s) 68, 253, 330, 945
HspAI GCGC 1 cut(s) 1125
Kzo9I GATC 4 cut(s) 6, 245, 358, 649
LmnI GCTCC 6 cut(s) 54, 84, 152, 539, 1060, 1386
Lsp1109I GCAGC 4 cut(s) 554, 610, 1028, 1091
LweI GCATC 2 cut(s) 714, 727
MaeII ACGT 4 cut(s) 68, 253, 330, 945
MaeIII GTNAC 1 cut(s) 946
MalI GATC 4 cut(s) 8, 247, 360, 651
MbiI CCGCTC 1 cut(s) 1389
MboI GATC 4 cut(s) 6, 245, 358, 649
MboII GAAGA 7 cut(s) 92, 221, 570, 844, 944, 975, 1207
MhlI GDGCHC 3 cut(s) 516, 1057, 1175
MluCI AATT 7 cut(s) 156, 161, 299, 313, 321, 966, 1315
MlyI GAGTC 2 cut(s) 181, 1247
MmeI TCCRAC 3 cut(s) 247, 396, 606
MslI CAYNNNNRTG 4 cut(s) 450, 563, 732, 1178
MspA1I CMGCKG 1 cut(s) 1295
MspI CCGG 4 cut(s) 789, 995, 1238, 1300
MspR9I CCNGG 6 cut(s) 191, 402, 995, 1194, 1203, 1238
MvaI CCWGG 4 cut(s) 191, 402, 1194, 1203
MvnI CGCG 1 cut(s) 238
MwoI GCNNNNNNNGC 3 cut(s) 632, 676, 1189
NciI CCSGG 2 cut(s) 995, 1238
NcoI CCATGG 4 cut(s) 445, 823, 1100, 1110
NdeII GATC 4 cut(s) 6, 245, 358, 649
NlaIV GGNNCC 7 cut(s) 514, 1056, 1098, 1118, 1235, 1290, 1325
NmuCI GTSAC 1 cut(s) 946
NspI RCATGY 1 cut(s) 1079
OliI CACNNNNGTG 1 cut(s) 1178
PaeI GCATGC 1 cut(s) 1079
PagI TCATGA 1 cut(s) 646
PasI CCCWGGG 1 cut(s) 401
PfeI GAWTC 2 cut(s) 659, 1085
PfoI TCCNGGA 1 cut(s) 189
PinAI ACCGGT 1 cut(s) 788
PkrI GCNGC 4 cut(s) 544, 625, 1043, 1081
PleI GAGTC 2 cut(s) 180, 1247
PmaCI CACGTG 1 cut(s) 254
PmlI CACGTG 1 cut(s) 254
PpsI GAGTC 2 cut(s) 180, 1247
Ppu21I YACGTR 2 cut(s) 254, 331
Psp6I CCWGG 4 cut(s) 189, 400, 1192, 1201
PspCI CACGTG 1 cut(s) 254
PspGI CCWGG 4 cut(s) 189, 400, 1192, 1201
PspN4I GGNNCC 7 cut(s) 514, 1056, 1098, 1118, 1235, 1290, 1325
PspOMI GGGCCC 1 cut(s) 512
PspPI GGNCC 7 cut(s) 512, 513, 791, 920, 1097, 1289, 1302
PstI CTGCAG 1 cut(s) 628
PvuII CAGCTG 1 cut(s) 1295
RsaI GTAC 2 cut(s) 119, 494
RsaNI GTAC 2 cut(s) 118, 493
RseI CAYNNNNRTG 4 cut(s) 450, 563, 732, 1178
SatI GCNGC 4 cut(s) 543, 624, 1042, 1080
Sau3AI GATC 4 cut(s) 6, 245, 358, 649
Sau96I GGNCC 7 cut(s) 512, 513, 791, 920, 1097, 1289, 1302
SchI GAGTC 2 cut(s) 181, 1247
ScrFI CCNGG 6 cut(s) 191, 402, 995, 1194, 1203, 1238
SduI GDGCHC 3 cut(s) 516, 1057, 1175
SfaNI GCATC 2 cut(s) 714, 727
SfcI CTRYAG 2 cut(s) 624, 808
SinI GGWCC 2 cut(s) 791, 920
SmiMI CAYNNNNRTG 4 cut(s) 450, 563, 732, 1178
SmlI CTYRAG 3 cut(s) 671, 1246, 1255
SmoI CTYRAG 3 cut(s) 671, 1246, 1255
SphI GCATGC 1 cut(s) 1079
Sse9I AATT 7 cut(s) 156, 161, 299, 313, 321, 966, 1315
SsiI CCGC 3 cut(s) 236, 1241, 1389
StyD4I CCNGG 6 cut(s) 189, 400, 993, 1192, 1201, 1236
StyI CCWWGG 5 cut(s) 272, 445, 823, 1100, 1110
TaaI ACNGT 2 cut(s) 186, 958
TaiI ACGT 4 cut(s) 71, 256, 333, 948
TaqI TCGA 4 cut(s) 166, 1147, 1308, 1394
TasI AATT 7 cut(s) 156, 161, 299, 313, 321, 966, 1315
TatI WGTACW 1 cut(s) 117
TfiI GAWTC 2 cut(s) 659, 1085
TscAI CASTG 4 cut(s) 189, 930, 1051, 1074
TseFI GTSAC 1 cut(s) 946
TseI GCWGC 4 cut(s) 542, 623, 1041, 1079
Tsp45I GTSAC 1 cut(s) 946
TspGWI ACGGA 1 cut(s) 29
TspRI CASTG 4 cut(s) 189, 930, 1051, 1074
VpaK11BI GGWCC 2 cut(s) 791, 920
XapI RAATTY 3 cut(s) 299, 313, 966
XceI RCATGY 1 cut(s) 1079
XcmI CCANNNNNNNNNTGG 1 cut(s) 821
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.