Rroxscaffold_2G00135670

Belongs to the glycosyl hydrolase 43 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0004682
Physical Location & Seq
Forward (+)
73254760 .. 73259256
4497 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00135670.1

Sequence Viewer

Length: 1491 bp
ATGAGGATGAGGAACAAATACAGGAAACCAACCACTTTCCATTGCCATGCAGGGAGCAGATGTTCAATATCTGCTGTGGTGTGGAGCTTAGTAGGATGTCTTCTTATGTTTCATTTCTACTCCCTAGTTCACCAGAAGGATGGGATGGGCAGAGAAATTCAATTTGGAGCAAGTCACCACCCACAGTTACATGAACTTGAAAAGGTGGAAGAGGAAAGTATCCACATTCCTCCACCAAGAAAGCGATCCCCACGTGCTGCAAAAAGAAAACCTAAGCGACCCACCACAATAATTGATGAATTTCTTGATGAGAATTCTCAAATTCGGCATGTATTCTTTCCTGATCAGAAACTTGCTATAGATCCGACGAAGGATGCGGGGAATGATAGCTATTACTATTACCCTGGAAGGATTTGGTTGGATACTGAAGGAAATCCTATTCAAGCCCATGGAGGTGGTATTCTGTATGATGAAAAATCAGGAACATACTATTGGTATGGGGAGTATAAAGATGGGCCTACGTACCATGCTCACAAAAAAGGAGCAGCCCGGGTTGACATCTTAGGAGTCGGTTGTTATTCTTCCAAGGACTTATGGAAATGGAACAATGAAGGTATTGTACTGGCAGCAGAAAAAACAAATGAAACACATGATCTCCATGAGTTGAATGTTCTTGAGAGGCCAAAAGTGATTTACAATCACAAGACAGCGAAGTATGTAATGTGGATGCATATTGATGATGTCAACTACACCAAAGCTTCTGTTGGCATTGCCATCAGCGATTACCCAACTGGTCCTTTTGATTATCTCTATAGCAAACGACCCCATGGATTTGAAAGTAGGGACATGACAATCTTCAAAGATGATGACGGTATTGCGTATCTAATATACTCCTCTGAGGACAATAGTGAACTTCACATTGGACCTCTAACTGACGATTATCTTGATGTGACCAACATCATGAGAAGAATTCTTGTGGGACAGCATCGAGAAGCCCCAGCTCTGTTCAAGCATGAGGGAACTTATTACATGATCACTTCAGGCTGTACAGGATGGGCACCAAATGAAGCACTGGCACATGCAGCAGAGTCAATCATGGGGCCATGGGAGACGATGGGAAACCCCTGCGTAGGAGGGAACAAAATGGCTCGACTTACAACATTTTTTGCGCAGAGCACATTTGTGATACCTTTACCAGGATTTCCTGGTTCATTTATTTTCATGGCAGACAGATGGAACCCCGCGGACTTGAGAGACTCAAGATATGTATGGTTGCCCTTGATAGTAGGGGGCCAGTTGATCGGCCCTTTGATTACAATTTTGGGTTCCCCTTGTGGTCAAGAGTGTCCATATATTGGCATCGGAAGTGGAAACTTCCTCTGGGGTGGAGTGGGTGGAAAAAGATATAGATTTTCTTTGTTTCATCTGTGTACCATATGTGAAGCATGGTTATTCTTTCACCGGCTCGCCATGATTGTTGCATTACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_43

Protein Analysis

496

Amino Acids

56.32

Weight (kDa)

6.91

Isoelectric Point (pI)

42.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_43 PF04616 188 - 372 7.1e-17 Glycosyl hydrolases family 43
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1170
AccB1I GGYRCC 1 cut(s) 1057
AccB7I CCANNNNNTGG 1 cut(s) 1355
AccII CGCG 1 cut(s) 1244
AciI CCGC 3 cut(s) 377, 1242, 1244
AclWI GGATC 2 cut(s) 240, 356
AcsI RAATTY 5 cut(s) 156, 299, 313, 321, 969
AcuI CTGAAG 2 cut(s) 447, 1023
AcvI CACGTG 1 cut(s) 254
AfaI GTAC 4 cut(s) 524, 621, 1048, 1432
AfiI CCNNNNNNNGG 3 cut(s) 1130, 1196, 1355
AgsI TTSAA 8 cut(s) 66, 161, 200, 443, 667, 836, 859, 1009
AjnI CCWGG 3 cut(s) 403, 1195, 1204
AjuI GAANNNNNNNTTGG 6 cut(s) 147, 179, 475, 507, 903, 935
AleI CACNNNNGTG 1 cut(s) 1181
AloI GAACNNNNNNTCC 2 cut(s) 46, 78
AluBI AGCT 4 cut(s) 87, 390, 758, 1001
AluI AGCT 4 cut(s) 87, 390, 758, 1001
Alw21I GWGCWC 1 cut(s) 1178
Alw26I GTCTC 2 cut(s) 1103, 1248
AlwI GGATC 2 cut(s) 240, 356
Ama87I CYCGRG 1 cut(s) 549
AoxI GGCC 5 cut(s) 515, 680, 1100, 1291, 1303
ApeKI GCWGC 4 cut(s) 257, 545, 626, 1082
ApoI RAATTY 5 cut(s) 156, 299, 313, 321, 969
AspLEI GCGC 1 cut(s) 1171
AspS9I GGNCC 6 cut(s) 515, 794, 923, 1100, 1291, 1304
AsuC2I CCSGG 2 cut(s) 550, 551
AsuHPI GGTGA 3 cut(s) 122, 167, 1451
AvaI CYCGRG 1 cut(s) 549
AvaII GGWCC 2 cut(s) 794, 923
BaeGI GKGCMC 1 cut(s) 1060
BanI GGYRCC 1 cut(s) 1057
BarI GAAGNNNNNNTAC 4 cut(s) 84, 116, 603, 635
BbrPI CACGTG 1 cut(s) 254
BbsI GAAGAC 1 cut(s) 92
Bbv12I GWGCWC 1 cut(s) 1178
BbvI GCAGC 4 cut(s) 244, 557, 638, 1094
BccI CCATC 7 cut(s) 134, 139, 506, 782, 1047, 1108, 1227
BciT130I CCWGG 3 cut(s) 405, 1197, 1206
BciVI GTATCC 2 cut(s) 230, 415
BclI TGATCA 2 cut(s) 343, 1032
BcnI CCSGG 2 cut(s) 550, 551
BcoDI GTCTC 2 cut(s) 1103, 1248
BfaI CTAG 1 cut(s) 125
BfmI CTRYAG 2 cut(s) 357, 811
BfuI GTATCC 2 cut(s) 230, 415
BisI GCNGC 4 cut(s) 258, 546, 627, 1083
BlsI GCNGC 4 cut(s) 259, 547, 628, 1084
Bme1390I CCNGG 5 cut(s) 405, 550, 551, 1197, 1206
Bme18I GGWCC 2 cut(s) 794, 923
BmeT110I CYCGRG 1 cut(s) 549
BmgT120I GGNCC 6 cut(s) 515, 794, 923, 1100, 1291, 1304
BmiI GGNNCC 5 cut(s) 1059, 1101, 1238, 1292, 1327
BmrFI CCNGG 5 cut(s) 405, 550, 551, 1197, 1206
BmsI GCATC 4 cut(s) 364, 717, 994, 1368
BpiI GAAGAC 1 cut(s) 92
Bpu10I CCTNAGC 1 cut(s) 273
BpuEI CTTGAG 3 cut(s) 695, 1243, 1270
BpuMI CCSGG 2 cut(s) 550, 551
BsaAI YACGTR 2 cut(s) 254, 522
BsaJI CCNNGG 7 cut(s) 403, 448, 549, 585, 826, 1103, 1242
BsaXI ACNNNNNCTCC 4 cut(s) 46, 76, 639, 669
Bsc4I CCNNNNNNNGG 3 cut(s) 1130, 1196, 1355
Bse118I RCCGGY 1 cut(s) 1461
Bse1I ACTGG 4 cut(s) 627, 796, 1077, 1294
Bse3DI GCAATG 2 cut(s) 40, 768
BseBI CCWGG 3 cut(s) 405, 1197, 1206
BseDI CCNNGG 7 cut(s) 403, 448, 549, 585, 826, 1103, 1242
BseGI GGATG 7 cut(s) 12, 101, 145, 150, 379, 732, 1058
BseLI CCNNNNNNNGG 3 cut(s) 1130, 1196, 1355
BseMI GCAATG 2 cut(s) 40, 768
BseMII CTCAG 1 cut(s) 888
BseNI ACTGG 4 cut(s) 627, 796, 1077, 1294
BseRI GAGGAG 1 cut(s) 883
BseSI GKGCMC 1 cut(s) 1060
BseXI GCAGC 4 cut(s) 244, 557, 638, 1094
BseYI CCCAGC 1 cut(s) 997
Bsh1236I CGCG 1 cut(s) 1244
BshFI GGCC 5 cut(s) 517, 682, 1102, 1293, 1305
BshNI GGYRCC 1 cut(s) 1057
BsiHKAI GWGCWC 1 cut(s) 1178
BsiHKCI CYCGRG 1 cut(s) 549
BsiSI CCGG 2 cut(s) 550, 1462
BslFI GGGAC 2 cut(s) 857, 993
BslI CCNNNNNNNGG 3 cut(s) 1130, 1196, 1355
BsmAI GTCTC 2 cut(s) 1103, 1248
BsmBI CGTCTC 1 cut(s) 1103
BsmFI GGGAC 2 cut(s) 857, 993
BsnI GGCC 5 cut(s) 517, 682, 1102, 1293, 1305
BsoBI CYCGRG 1 cut(s) 549
Bsp1286I GDGCHC 2 cut(s) 1060, 1178
Bsp1407I TGTACA 1 cut(s) 1046
Bsp143I GATC 6 cut(s) 245, 343, 361, 652, 1032, 1299
Bsp19I CCATGG 3 cut(s) 448, 826, 1103
BspACI CCGC 3 cut(s) 377, 1242, 1244
BspANI GGCC 5 cut(s) 517, 682, 1102, 1293, 1305
BspCNI CTCAG 1 cut(s) 889
BspFNI CGCG 1 cut(s) 1244
BspHI TCATGA 1 cut(s) 960
BspLI GGNNCC 5 cut(s) 1059, 1101, 1238, 1292, 1327
BspPI GGATC 2 cut(s) 240, 356
BspT107I GGYRCC 1 cut(s) 1057
BsrDI GCAATG 2 cut(s) 40, 768
BsrFI RCCGGY 1 cut(s) 1461
BsrGI TGTACA 1 cut(s) 1046
BsrI ACTGG 4 cut(s) 627, 796, 1077, 1294
BssAI RCCGGY 1 cut(s) 1461
BssECI CCNNGG 7 cut(s) 403, 448, 549, 585, 826, 1103, 1242
BssMI GATC 6 cut(s) 245, 343, 361, 652, 1032, 1299
BssT1I CCWWGG 4 cut(s) 448, 585, 826, 1103
Bst2UI CCWGG 3 cut(s) 405, 1197, 1206
Bst4CI ACNGT 3 cut(s) 186, 872, 1488
Bst6I CTCTTC 1 cut(s) 204
BstAUI TGTACA 1 cut(s) 1046
BstBAI YACGTR 2 cut(s) 254, 522
BstC8I GCNNGC 1 cut(s) 1467
BstDEI CTNAG 4 cut(s) 88, 273, 562, 897
BstDSI CCRYGG 4 cut(s) 448, 826, 1103, 1242
BstENI CCTNNNNNAGG 1 cut(s) 1194
BstF5I GGATG 7 cut(s) 12, 101, 145, 150, 379, 732, 1058
BstFNI CGCG 1 cut(s) 1244
BstHHI GCGC 1 cut(s) 1171
BstKTI GATC 6 cut(s) 248, 346, 364, 655, 1035, 1302
BstMAI GTCTC 2 cut(s) 1103, 1248
BstMBI GATC 6 cut(s) 245, 343, 361, 652, 1032, 1299
BstMWI GCNNNNNNNGC 1 cut(s) 1082
BstNI CCWGG 3 cut(s) 405, 1197, 1206
BstNSI RCATGY 2 cut(s) 332, 1082
BstSCI CCNGG 5 cut(s) 403, 548, 549, 1195, 1204
BstSFI CTRYAG 2 cut(s) 357, 811
BstSLI GKGCMC 1 cut(s) 1060
BstSNI TACGTA 1 cut(s) 522
BstUI CGCG 1 cut(s) 1244
BstV1I GCAGC 4 cut(s) 244, 557, 638, 1094
BstV2I GAAGAC 1 cut(s) 92
BstX2I RGATCY 1 cut(s) 361
BstXI CCANNNNNNTGG 2 cut(s) 140, 455
BstYI RGATCY 1 cut(s) 361
BsuI GTATCC 2 cut(s) 230, 415
BsuRI GGCC 5 cut(s) 517, 682, 1102, 1293, 1305
BtgI CCRYGG 4 cut(s) 448, 826, 1103, 1242
BtsCI GGATG 7 cut(s) 12, 101, 145, 150, 379, 732, 1058
BtsIMutI CAGTG 1 cut(s) 1070
Cac8I GCNNGC 1 cut(s) 1467
CciI TCATGA 1 cut(s) 960
CfoI GCGC 1 cut(s) 1171
Cfr10I RCCGGY 1 cut(s) 1461
Cfr13I GGNCC 6 cut(s) 515, 794, 923, 1100, 1291, 1304
Cfr42I CCGCGG 1 cut(s) 1245
Cfr9I CCCGGG 1 cut(s) 549
Csp6I GTAC 4 cut(s) 523, 620, 1047, 1431
CspCI CAANNNNNGTGG 2 cut(s) 274, 309
CviQI GTAC 4 cut(s) 523, 620, 1047, 1431
DdeI CTNAG 4 cut(s) 88, 273, 562, 897
DpnI GATC 6 cut(s) 247, 345, 363, 654, 1034, 1301
DpnII GATC 6 cut(s) 245, 343, 361, 652, 1032, 1299
Eam1104I CTCTTC 1 cut(s) 204
EarI CTCTTC 1 cut(s) 204
Eco105I TACGTA 1 cut(s) 522
Eco130I CCWWGG 4 cut(s) 448, 585, 826, 1103
Eco47I GGWCC 2 cut(s) 794, 923
Eco57I CTGAAG 2 cut(s) 447, 1023
Eco72I CACGTG 1 cut(s) 254
Eco88I CYCGRG 1 cut(s) 549
EcoNI CCTNNNNNAGG 1 cut(s) 1194
EcoRI GAATTC 2 cut(s) 313, 969
EcoRII CCWGG 3 cut(s) 403, 1195, 1204
EcoT14I CCWWGG 4 cut(s) 448, 585, 826, 1103
EcoT22I ATGCAT 1 cut(s) 732
ErhI CCWWGG 4 cut(s) 448, 585, 826, 1103
Esp3I CGTCTC 1 cut(s) 1103
FaqI GGGAC 2 cut(s) 857, 993
FauI CCCGC 2 cut(s) 370, 1249
FauNDI CATATG 1 cut(s) 1436
FbaI TGATCA 2 cut(s) 343, 1032
Fnu4HI GCNGC 4 cut(s) 258, 546, 627, 1083
FokI GGATG 7 cut(s) 19, 108, 152, 157, 386, 739, 1065
Fsp4HI GCNGC 4 cut(s) 258, 546, 627, 1083
FspBI CTAG 1 cut(s) 125
FspI TGCGCA 1 cut(s) 1170
GlaI GCGC 1 cut(s) 1170
GluI GCNGC 4 cut(s) 258, 546, 627, 1083
GsaI CCCAGC 1 cut(s) 1001
HaeIII GGCC 5 cut(s) 517, 682, 1102, 1293, 1305
HapII CCGG 2 cut(s) 550, 1462
HhaI GCGC 1 cut(s) 1171
Hin6I GCGC 1 cut(s) 1169
HinP1I GCGC 1 cut(s) 1169
HincII GTYRAC 2 cut(s) 556, 745
HindII GTYRAC 2 cut(s) 556, 745
HindIII AAGCTT 1 cut(s) 756
HinfI GANTC 3 cut(s) 567, 1088, 1256
HpaII CCGG 2 cut(s) 550, 1462
HphI GGTGA 3 cut(s) 122, 167, 1451
Hpy166II GTNNAC 5 cut(s) 130, 556, 745, 911, 1431
Hpy188I TCNGA 4 cut(s) 348, 366, 898, 1364
Hpy188III TCNNGA 9 cut(s) 305, 341, 480, 674, 944, 961, 989, 1260, 1340
Hpy8I GTNNAC 5 cut(s) 130, 556, 745, 911, 1431
Hpy99I CGWCG 1 cut(s) 370
HpyAV CCTTC 5 cut(s) 130, 364, 402, 422, 605
HpyCH4III ACNGT 3 cut(s) 186, 872, 1488
HpyCH4IV ACGT 2 cut(s) 253, 521
HpyCH4V TGCA 5 cut(s) 50, 260, 730, 1082, 1481
HpyF10VI GCNNNNNNNGC 1 cut(s) 1082
HpyF3I CTNAG 4 cut(s) 88, 273, 562, 897
HpySE526I ACGT 2 cut(s) 253, 521
HspAI GCGC 1 cut(s) 1169
Ksp22I TGATCA 2 cut(s) 343, 1032
KspI CCGCGG 1 cut(s) 1245
Kzo9I GATC 6 cut(s) 245, 343, 361, 652, 1032, 1299
LmnI GCTCC 4 cut(s) 54, 84, 167, 542
Lsp1109I GCAGC 4 cut(s) 244, 557, 638, 1094
LweI GCATC 4 cut(s) 364, 717, 994, 1368
MaeI CTAG 1 cut(s) 125
MaeII ACGT 2 cut(s) 253, 521
MaeIII GTNAC 3 cut(s) 173, 186, 949
MalI GATC 6 cut(s) 247, 345, 363, 654, 1034, 1301
MboI GATC 6 cut(s) 245, 343, 361, 652, 1032, 1299
MboII GAAGA 5 cut(s) 92, 221, 573, 847, 978
MflI RGATCY 1 cut(s) 361
MhlI GDGCHC 2 cut(s) 1060, 1178
MluCI AATT 8 cut(s) 156, 161, 291, 299, 313, 321, 969, 1317
MlyI GAGTC 3 cut(s) 576, 1097, 1250
MmeI TCCRAC 2 cut(s) 389, 399
Mph1103I ATGCAT 1 cut(s) 732
MslI CAYNNNNRTG 4 cut(s) 45, 453, 735, 1181
MspA1I CMGCKG 1 cut(s) 1244
MspI CCGG 2 cut(s) 550, 1462
MspR9I CCNGG 5 cut(s) 405, 550, 551, 1197, 1206
MvaI CCWGG 3 cut(s) 405, 1197, 1206
MvnI CGCG 1 cut(s) 1244
MwoI GCNNNNNNNGC 1 cut(s) 1082
NciI CCSGG 2 cut(s) 550, 551
NcoI CCATGG 3 cut(s) 448, 826, 1103
NdeI CATATG 1 cut(s) 1436
NdeII GATC 6 cut(s) 245, 343, 361, 652, 1032, 1299
NlaIV GGNNCC 5 cut(s) 1059, 1101, 1238, 1292, 1327
NmuCI GTSAC 2 cut(s) 173, 949
NsbI TGCGCA 1 cut(s) 1170
NsiI ATGCAT 1 cut(s) 732
NspI RCATGY 2 cut(s) 332, 1082
OliI CACNNNNGTG 1 cut(s) 1181
PagI TCATGA 1 cut(s) 960
PflMI CCANNNNNTGG 1 cut(s) 1355
PkrI GCNGC 4 cut(s) 259, 547, 628, 1084
PleI GAGTC 3 cut(s) 575, 1096, 1250
PmaCI CACGTG 1 cut(s) 254
PmlI CACGTG 1 cut(s) 254
PpsI GAGTC 3 cut(s) 575, 1096, 1250
Ppu21I YACGTR 2 cut(s) 254, 522
Psp6I CCWGG 3 cut(s) 403, 1195, 1204
PspCI CACGTG 1 cut(s) 254
PspFI CCCAGC 1 cut(s) 997
PspGI CCWGG 3 cut(s) 403, 1195, 1204
PspN4I GGNNCC 5 cut(s) 1059, 1101, 1238, 1292, 1327
PspPI GGNCC 6 cut(s) 515, 794, 923, 1100, 1291, 1304
PsuI RGATCY 1 cut(s) 361
RsaI GTAC 4 cut(s) 524, 621, 1048, 1432
RsaNI GTAC 4 cut(s) 523, 620, 1047, 1431
RseI CAYNNNNRTG 4 cut(s) 45, 453, 735, 1181
SacII CCGCGG 1 cut(s) 1245
SatI GCNGC 4 cut(s) 258, 546, 627, 1083
Sau3AI GATC 6 cut(s) 245, 343, 361, 652, 1032, 1299
Sau96I GGNCC 6 cut(s) 515, 794, 923, 1100, 1291, 1304
SchI GAGTC 3 cut(s) 576, 1097, 1250
ScrFI CCNGG 5 cut(s) 405, 550, 551, 1197, 1206
SduI GDGCHC 2 cut(s) 1060, 1178
SfaNI GCATC 4 cut(s) 364, 717, 994, 1368
SfcI CTRYAG 2 cut(s) 357, 811
Sfr303I CCGCGG 1 cut(s) 1245
SgrBI CCGCGG 1 cut(s) 1245
SinI GGWCC 2 cut(s) 794, 923
SmaI CCCGGG 1 cut(s) 551
SmiMI CAYNNNNRTG 4 cut(s) 45, 453, 735, 1181
SmlI CTYRAG 3 cut(s) 674, 1249, 1258
SmoI CTYRAG 3 cut(s) 674, 1249, 1258
SnaBI TACGTA 1 cut(s) 522
Sse9I AATT 8 cut(s) 156, 161, 291, 299, 313, 321, 969, 1317
SsiI CCGC 3 cut(s) 377, 1242, 1244
SspMI CTAG 1 cut(s) 125
StyD4I CCNGG 5 cut(s) 403, 548, 549, 1195, 1204
StyI CCWWGG 4 cut(s) 448, 585, 826, 1103
TaaI ACNGT 3 cut(s) 186, 872, 1488
TaiI ACGT 2 cut(s) 256, 524
TaqI TCGA 2 cut(s) 988, 1150
TasI AATT 8 cut(s) 156, 161, 291, 299, 313, 321, 969, 1317
TatI WGTACW 2 cut(s) 619, 1046
TscAI CASTG 1 cut(s) 1077
TseFI GTSAC 2 cut(s) 173, 949
TseI GCWGC 4 cut(s) 257, 545, 626, 1082
Tsp45I GTSAC 2 cut(s) 173, 949
TspMI CCCGGG 1 cut(s) 549
TspRI CASTG 1 cut(s) 1077
Van91I CCANNNNNTGG 1 cut(s) 1355
VpaK11BI GGWCC 2 cut(s) 794, 923
XagI CCTNNNNNAGG 1 cut(s) 1194
XapI RAATTY 5 cut(s) 156, 299, 313, 321, 969
XceI RCATGY 2 cut(s) 332, 1082
XmaI CCCGGG 1 cut(s) 549
XspI CTAG 1 cut(s) 125
Zsp2I ATGCAT 1 cut(s) 732
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.