FvH4_1g17720

Belongs to the glycosyl hydrolase 43 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0004682
Physical Location & Seq
Reverse (-)
10311542 .. 10316052
4511 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g17720.t2

Sequence Viewer

Length: 1404 bp
ATGAGGAACAAATACAGGAAACCAACCACTTTCCGTTGCTATGCAGGGAGCAGATGTTCCATATCTGCTGTCGTGTGGAGCTTGGTAGGATGTCTTCTTATGTTTCATTTATACTCCCTAGTTCACCACAAGGATGGGATGGGCAGAGAAATTCAGTTTCGAGCAAGTGTCCACCCACAGTTACATGAACTTGAAAAGGTGGAAGAGGAAAGTATCCGCATGCCTCCACCAAGAAAGCGATCCCCACGTGCTGCAAAAAGAAAACCTAAGCGACCCACCACAATAATCGATGAATTTCTTGATGAGAATTCTCAAATTCGGCACGTATTCTTTCCTGACCAGAAGCTTGCTATAGATCCATTGAAGGATGCTGGGAATGATAGCTATTACTATTACCCTGGAAGGATTTGGTTGGACACTGAAGAAAATCCTATTCAAGCCCATGGAGGTGGTATTTTATATGATGAAAAATCAGGAACATACTATTGGTATGGGGAGTATAAAGATGGGCCCACGTACCATGCTCACAAAAAAGGAGCTGCCCGGGTTGACATTTTAGGAGTCGGTTGTTATTCTTCCAAGGACTTATGGAAATGGAACAATGAAGGTATTGTACTGGCAGCAGAAAAAACAAACGAAACACATGATCTCCACGAGTTGAATGTTCTCGAGAGGCCAAAAGTGATTTACAATCACAAGACAGCAAAGTATGTAATGTGGATGCATATTGATGATGTCAACTACACCAAAGCCTCTGTTGGTGTAGCCATCAGTGATTACCCAACCGGTCCTTTTGATTATCTCTACAGCAAACGACCCCATGGATTTGATAGTAGGGACATGACAGTCTTCAAAGATGATGATGGTATTGCATATCTAATATACTCCTCTGATGACAATAGTGAACTTCACATTGGACCTCTAACTGAAGATTATCTTGATGTGACAAACATTGTGAGAAGAATTCTTGTCGGACAGCATCGGGAAGCCCCAGCTTTGTTCAAGCATGATGGAACTTATTACATGATCACTTCAGGCTGCACAGGATGGGCACCAAATGAAGCAATGGCTCATGCAGCAGAGTCAATCATGGGGCCTTGGGAGACTATGGGAAACCCCTGTATAGGAGGGAACAAAATGTCTCGACTTGCAACATTTTTTGCACAGAGCACATTTGTGATACCTTTACCGGGATTTCCTGGTTCATTTATTTTCATGGCAGACAGATGGAACCCTGCAGACTTGAGAGACTCGAGATATGTATGGTTGCCCTTGATAGTAGGGGGGCCGGTTGATCGGCCCCTGGATTACAATTTTGGGTTCCCGTTGTGGTCTAGAGTATCGATATATTGGCATAGGAAGTGGAAACTTCCTCAGGGTTGGAGTGGGTGGAAAAAGATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_43

Protein Analysis

468

Amino Acids

53.77

Weight (kDa)

8.05

Isoelectric Point (pI)

47.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_43 PF04616 177 - 361 5.7e-18 Glycosyl hydrolases family 43
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 845
AccB1I GGYRCC 1 cut(s) 1051
AciI CCGC 1 cut(s) 217
AclWI GGATC 2 cut(s) 234, 350
AcsI RAATTY 5 cut(s) 150, 293, 307, 315, 963
AcuI CTGAAG 3 cut(s) 441, 948, 1017
AcvI CACGTG 1 cut(s) 248
AfaI GTAC 2 cut(s) 518, 615
AfiI CCNNNNNNNGG 2 cut(s) 1124, 1190
AgeI ACCGGT 1 cut(s) 785
AgsI TTSAA 6 cut(s) 194, 364, 437, 661, 853, 1003
AjnI CCWGG 3 cut(s) 397, 1198, 1302
AjuI GAANNNNNNNTTGG 4 cut(s) 469, 501, 897, 929
AleI CACNNNNGTG 1 cut(s) 1175
AloI GAACNNNNNNTCC 2 cut(s) 40, 72
AluBI AGCT 5 cut(s) 81, 346, 384, 539, 995
AluI AGCT 5 cut(s) 81, 346, 384, 539, 995
Alw21I GWGCWC 1 cut(s) 1172
Alw26I GTCTC 3 cut(s) 1097, 1146, 1242
AlwI GGATC 2 cut(s) 234, 350
Ama87I CYCGRG 3 cut(s) 543, 668, 1252
AoxI GGCC 5 cut(s) 509, 674, 1094, 1286, 1298
ApaI GGGCCC 1 cut(s) 513
ApeKI GCWGC 5 cut(s) 251, 539, 620, 1038, 1076
ApoI RAATTY 5 cut(s) 150, 293, 307, 315, 963
AsiGI ACCGGT 1 cut(s) 785
AspS9I GGNCC 7 cut(s) 509, 510, 788, 917, 1094, 1286, 1299
AsuC2I CCSGG 3 cut(s) 544, 545, 1191
AsuHPI GGTGA 1 cut(s) 116
AvaI CYCGRG 3 cut(s) 543, 668, 1252
AvaII GGWCC 2 cut(s) 788, 917
AxyI CCTNAGG 1 cut(s) 1374
BaeGI GKGCMC 2 cut(s) 513, 1054
BanI GGYRCC 1 cut(s) 1051
BanII GRGCYC 1 cut(s) 513
BarI GAAGNNNNNNTAC 4 cut(s) 78, 110, 597, 629
BauI CACGAG 1 cut(s) 653
BbrPI CACGTG 1 cut(s) 248
BbsI GAAGAC 2 cut(s) 86, 841
Bbv12I GWGCWC 1 cut(s) 1172
BbvI GCAGC 5 cut(s) 238, 526, 632, 1025, 1088
BccI CCATC 8 cut(s) 128, 133, 500, 776, 857, 1004, 1041, 1221
BciT130I CCWGG 3 cut(s) 399, 1200, 1304
BciVI GTATCC 1 cut(s) 224
BclI TGATCA 1 cut(s) 1026
BcnI CCSGG 3 cut(s) 544, 545, 1191
BcoDI GTCTC 3 cut(s) 1097, 1146, 1242
BfaI CTAG 2 cut(s) 119, 1335
BfmI CTRYAG 3 cut(s) 351, 805, 1236
BfuI GTATCC 1 cut(s) 224
BisI GCNGC 5 cut(s) 252, 540, 621, 1039, 1077
BlsI GCNGC 5 cut(s) 253, 541, 622, 1040, 1078
Bme1390I CCNGG 6 cut(s) 399, 544, 545, 1191, 1200, 1304
Bme18I GGWCC 2 cut(s) 788, 917
BmeT110I CYCGRG 3 cut(s) 543, 668, 1252
BmgT120I GGNCC 7 cut(s) 509, 510, 788, 917, 1094, 1286, 1299
BmiI GGNNCC 7 cut(s) 511, 1053, 1095, 1232, 1287, 1301, 1322
BmrFI CCNGG 6 cut(s) 399, 544, 545, 1191, 1200, 1304
BmsI GCATC 3 cut(s) 358, 711, 988
BpiI GAAGAC 2 cut(s) 86, 841
Bpu10I CCTNAGC 1 cut(s) 267
BpuEI CTTGAG 1 cut(s) 1264
BpuMI CCSGG 3 cut(s) 544, 545, 1191
Bsa29I ATCGAT 2 cut(s) 288, 1343
BsaAI YACGTR 3 cut(s) 248, 325, 516
BsaJI CCNNGG 7 cut(s) 397, 442, 543, 579, 820, 1097, 1302
BsaWI WCCGGW 1 cut(s) 785
BsaXI ACNNNNNCTCC 4 cut(s) 40, 70, 633, 663
Bsc4I CCNNNNNNNGG 2 cut(s) 1124, 1190
Bse118I RCCGGY 2 cut(s) 785, 1288
Bse1I ACTGG 1 cut(s) 621
Bse21I CCTNAGG 1 cut(s) 1374
Bse3DI GCAATG 1 cut(s) 1071
BseBI CCWGG 3 cut(s) 399, 1200, 1304
BseCI ATCGAT 2 cut(s) 288, 1343
BseDI CCNNGG 7 cut(s) 397, 442, 543, 579, 820, 1097, 1302
BseGI GGATG 6 cut(s) 95, 139, 144, 373, 726, 1052
BseLI CCNNNNNNNGG 2 cut(s) 1124, 1190
BseMI GCAATG 1 cut(s) 1071
BseMII CTCAG 1 cut(s) 1388
BseNI ACTGG 1 cut(s) 621
BseRI GAGGAG 1 cut(s) 877
BseSI GKGCMC 2 cut(s) 513, 1054
BseXI GCAGC 5 cut(s) 238, 526, 632, 1025, 1088
BseYI CCCAGC 2 cut(s) 371, 991
BsgI GTGCAG 1 cut(s) 1024
BshFI GGCC 5 cut(s) 511, 676, 1096, 1288, 1300
BshNI GGYRCC 1 cut(s) 1051
BshTI ACCGGT 1 cut(s) 785
BshVI ATCGAT 2 cut(s) 288, 1343
BsiHKAI GWGCWC 1 cut(s) 1172
BsiHKCI CYCGRG 3 cut(s) 543, 668, 1252
BsiSI CCGG 4 cut(s) 544, 786, 1190, 1289
BslFI GGGAC 1 cut(s) 851
BslI CCNNNNNNNGG 2 cut(s) 1124, 1190
BsmAI GTCTC 3 cut(s) 1097, 1146, 1242
BsmFI GGGAC 1 cut(s) 851
BsnI GGCC 5 cut(s) 511, 676, 1096, 1288, 1300
BsoBI CYCGRG 3 cut(s) 543, 668, 1252
Bsp120I GGGCCC 1 cut(s) 509
Bsp1286I GDGCHC 3 cut(s) 513, 1054, 1172
Bsp143I GATC 5 cut(s) 239, 355, 646, 1026, 1294
Bsp19I CCATGG 2 cut(s) 442, 820
BspACI CCGC 1 cut(s) 217
BspANI GGCC 5 cut(s) 511, 676, 1096, 1288, 1300
BspCNI CTCAG 1 cut(s) 1387
BspDI ATCGAT 2 cut(s) 288, 1343
BspLI GGNNCC 7 cut(s) 511, 1053, 1095, 1232, 1287, 1301, 1322
BspMAI CTGCAG 1 cut(s) 1240
BspPI GGATC 2 cut(s) 234, 350
BspT107I GGYRCC 1 cut(s) 1051
BsrDI GCAATG 1 cut(s) 1071
BsrFI RCCGGY 2 cut(s) 785, 1288
BsrI ACTGG 1 cut(s) 621
BssAI RCCGGY 2 cut(s) 785, 1288
BssECI CCNNGG 7 cut(s) 397, 442, 543, 579, 820, 1097, 1302
BssMI GATC 5 cut(s) 239, 355, 646, 1026, 1294
BssSI CACGAG 1 cut(s) 653
BssT1I CCWWGG 4 cut(s) 442, 579, 820, 1097
Bst2BI CACGAG 1 cut(s) 653
Bst2UI CCWGG 3 cut(s) 399, 1200, 1304
Bst4CI ACNGT 2 cut(s) 180, 847
Bst6I CTCTTC 1 cut(s) 198
BstBAI YACGTR 3 cut(s) 248, 325, 516
BstC8I GCNNGC 2 cut(s) 221, 348
BstDEI CTNAG 2 cut(s) 267, 1374
BstDSI CCRYGG 2 cut(s) 442, 820
BstF5I GGATG 6 cut(s) 95, 139, 144, 373, 726, 1052
BstKTI GATC 5 cut(s) 242, 358, 649, 1029, 1297
BstMAI GTCTC 3 cut(s) 1097, 1146, 1242
BstMBI GATC 5 cut(s) 239, 355, 646, 1026, 1294
BstMWI GCNNNNNNNGC 1 cut(s) 1076
BstNI CCWGG 3 cut(s) 399, 1200, 1304
BstNSI RCATGY 1 cut(s) 223
BstSCI CCNGG 6 cut(s) 397, 542, 543, 1189, 1198, 1302
BstSFI CTRYAG 3 cut(s) 351, 805, 1236
BstSLI GKGCMC 2 cut(s) 513, 1054
BstV1I GCAGC 5 cut(s) 238, 526, 632, 1025, 1088
BstV2I GAAGAC 2 cut(s) 86, 841
BstX2I RGATCY 1 cut(s) 355
BstXI CCANNNNNNTGG 2 cut(s) 134, 449
BstYI RGATCY 1 cut(s) 355
Bsu15I ATCGAT 2 cut(s) 288, 1343
Bsu36I CCTNAGG 1 cut(s) 1374
BsuI GTATCC 1 cut(s) 224
BsuRI GGCC 5 cut(s) 511, 676, 1096, 1288, 1300
BsuTUI ATCGAT 2 cut(s) 288, 1343
BtgI CCRYGG 2 cut(s) 442, 820
BtsCI GGATG 6 cut(s) 95, 139, 144, 373, 726, 1052
BtsIMutI CAGTG 2 cut(s) 417, 778
Cac8I GCNNGC 2 cut(s) 221, 348
Cfr10I RCCGGY 2 cut(s) 785, 1288
Cfr13I GGNCC 7 cut(s) 509, 510, 788, 917, 1094, 1286, 1299
Cfr9I CCCGGG 1 cut(s) 543
ClaI ATCGAT 2 cut(s) 288, 1343
Csp6I GTAC 2 cut(s) 517, 614
CspAI ACCGGT 1 cut(s) 785
CviQI GTAC 2 cut(s) 517, 614
DdeI CTNAG 2 cut(s) 267, 1374
DpnI GATC 5 cut(s) 241, 357, 648, 1028, 1296
DpnII GATC 5 cut(s) 239, 355, 646, 1026, 1294
DrdI GACNNNNNNGTC 1 cut(s) 845
DseDI GACNNNNNNGTC 1 cut(s) 845
Eam1104I CTCTTC 1 cut(s) 198
EarI CTCTTC 1 cut(s) 198
Eco130I CCWWGG 4 cut(s) 442, 579, 820, 1097
Eco24I GRGCYC 1 cut(s) 513
Eco47I GGWCC 2 cut(s) 788, 917
Eco57I CTGAAG 3 cut(s) 441, 948, 1017
Eco72I CACGTG 1 cut(s) 248
Eco81I CCTNAGG 1 cut(s) 1374
Eco88I CYCGRG 3 cut(s) 543, 668, 1252
EcoO109I RGGNCCY 1 cut(s) 1094
EcoRI GAATTC 2 cut(s) 307, 963
EcoRII CCWGG 3 cut(s) 397, 1198, 1302
EcoT14I CCWWGG 4 cut(s) 442, 579, 820, 1097
EcoT22I ATGCAT 1 cut(s) 726
EcoT38I GRGCYC 1 cut(s) 513
ErhI CCWWGG 4 cut(s) 442, 579, 820, 1097
FalI AAGNNNNNCTT 2 cut(s) 921, 953
FaqI GGGAC 1 cut(s) 851
FbaI TGATCA 1 cut(s) 1026
Fnu4HI GCNGC 5 cut(s) 252, 540, 621, 1039, 1077
FokI GGATG 6 cut(s) 102, 146, 151, 380, 733, 1059
FriOI GRGCYC 1 cut(s) 513
Fsp4HI GCNGC 5 cut(s) 252, 540, 621, 1039, 1077
FspBI CTAG 2 cut(s) 119, 1335
GluI GCNGC 5 cut(s) 252, 540, 621, 1039, 1077
GsaI CCCAGC 2 cut(s) 375, 995
HaeIII GGCC 5 cut(s) 511, 676, 1096, 1288, 1300
HapII CCGG 4 cut(s) 544, 786, 1190, 1289
HincII GTYRAC 2 cut(s) 550, 739
HindII GTYRAC 2 cut(s) 550, 739
HindIII AAGCTT 1 cut(s) 344
HinfI GANTC 3 cut(s) 561, 1082, 1250
HpaII CCGG 4 cut(s) 544, 786, 1190, 1289
HphI GGTGA 1 cut(s) 116
Hpy166II GTNNAC 5 cut(s) 124, 172, 550, 739, 905
Hpy188I TCNGA 2 cut(s) 892, 974
Hpy8I GTNNAC 5 cut(s) 124, 172, 550, 739, 905
HpyAV CCTTC 3 cut(s) 358, 396, 599
HpyCH4III ACNGT 2 cut(s) 180, 847
HpyCH4IV ACGT 3 cut(s) 247, 324, 515
HpyCH4V TGCA 9 cut(s) 44, 254, 724, 872, 1041, 1076, 1151, 1163, 1238
HpyF10VI GCNNNNNNNGC 1 cut(s) 1076
HpyF3I CTNAG 2 cut(s) 267, 1374
HpySE526I ACGT 3 cut(s) 247, 324, 515
Ksp22I TGATCA 1 cut(s) 1026
Kzo9I GATC 5 cut(s) 239, 355, 646, 1026, 1294
LmnI GCTCC 3 cut(s) 48, 78, 536
Lsp1109I GCAGC 5 cut(s) 238, 526, 632, 1025, 1088
LweI GCATC 3 cut(s) 358, 711, 988
MaeI CTAG 2 cut(s) 119, 1335
MaeII ACGT 3 cut(s) 247, 324, 515
MaeIII GTNAC 2 cut(s) 180, 943
MalI GATC 5 cut(s) 241, 357, 648, 1028, 1296
MboI GATC 5 cut(s) 239, 355, 646, 1026, 1294
MboII GAAGA 7 cut(s) 86, 215, 434, 567, 841, 941, 972
MflI RGATCY 1 cut(s) 355
MhlI GDGCHC 3 cut(s) 513, 1054, 1172
MluCI AATT 6 cut(s) 150, 293, 307, 315, 963, 1312
MlyI GAGTC 3 cut(s) 570, 1091, 1244
MmeI TCCRAC 3 cut(s) 393, 952, 1361
MnlI CCTC 9 cut(s) 199, 234, 440, 666, 763, 898, 930, 1121, 1383
Mph1103I ATGCAT 1 cut(s) 726
MslI CAYNNNNRTG 4 cut(s) 132, 447, 729, 1175
MspI CCGG 4 cut(s) 544, 786, 1190, 1289
MspR9I CCNGG 6 cut(s) 399, 544, 545, 1191, 1200, 1304
MvaI CCWGG 3 cut(s) 399, 1200, 1304
MwoI GCNNNNNNNGC 1 cut(s) 1076
NciI CCSGG 3 cut(s) 544, 545, 1191
NcoI CCATGG 2 cut(s) 442, 820
NdeII GATC 5 cut(s) 239, 355, 646, 1026, 1294
NlaIV GGNNCC 7 cut(s) 511, 1053, 1095, 1232, 1287, 1301, 1322
NmuCI GTSAC 1 cut(s) 943
NsiI ATGCAT 1 cut(s) 726
NspI RCATGY 1 cut(s) 223
OliI CACNNNNGTG 1 cut(s) 1175
PaeI GCATGC 1 cut(s) 223
PaeR7I CTCGAG 2 cut(s) 668, 1252
PinAI ACCGGT 1 cut(s) 785
PkrI GCNGC 5 cut(s) 253, 541, 622, 1040, 1078
PleI GAGTC 3 cut(s) 569, 1090, 1244
PmaCI CACGTG 1 cut(s) 248
PmlI CACGTG 1 cut(s) 248
PpsI GAGTC 3 cut(s) 569, 1090, 1244
Ppu21I YACGTR 3 cut(s) 248, 325, 516
Psp6I CCWGG 3 cut(s) 397, 1198, 1302
PspCI CACGTG 1 cut(s) 248
PspFI CCCAGC 2 cut(s) 371, 991
PspGI CCWGG 3 cut(s) 397, 1198, 1302
PspN4I GGNNCC 7 cut(s) 511, 1053, 1095, 1232, 1287, 1301, 1322
PspOMI GGGCCC 1 cut(s) 509
PspPI GGNCC 7 cut(s) 509, 510, 788, 917, 1094, 1286, 1299
PstI CTGCAG 1 cut(s) 1240
PsuI RGATCY 1 cut(s) 355
RsaI GTAC 2 cut(s) 518, 615
RsaNI GTAC 2 cut(s) 517, 614
RseI CAYNNNNRTG 4 cut(s) 132, 447, 729, 1175
SatI GCNGC 5 cut(s) 252, 540, 621, 1039, 1077
Sau3AI GATC 5 cut(s) 239, 355, 646, 1026, 1294
Sau96I GGNCC 7 cut(s) 509, 510, 788, 917, 1094, 1286, 1299
SchI GAGTC 3 cut(s) 570, 1091, 1244
ScrFI CCNGG 6 cut(s) 399, 544, 545, 1191, 1200, 1304
SduI GDGCHC 3 cut(s) 513, 1054, 1172
SfaNI GCATC 3 cut(s) 358, 711, 988
SfcI CTRYAG 3 cut(s) 351, 805, 1236
Sfr274I CTCGAG 2 cut(s) 668, 1252
SinI GGWCC 2 cut(s) 788, 917
SlaI CTCGAG 2 cut(s) 668, 1252
SmaI CCCGGG 1 cut(s) 545
SmiMI CAYNNNNRTG 4 cut(s) 132, 447, 729, 1175
SmlI CTYRAG 3 cut(s) 668, 1243, 1252
SmoI CTYRAG 3 cut(s) 668, 1243, 1252
SphI GCATGC 1 cut(s) 223
Sse9I AATT 6 cut(s) 150, 293, 307, 315, 963, 1312
SsiI CCGC 1 cut(s) 217
SspMI CTAG 2 cut(s) 119, 1335
StyD4I CCNGG 6 cut(s) 397, 542, 543, 1189, 1198, 1302
StyI CCWWGG 4 cut(s) 442, 579, 820, 1097
TaaI ACNGT 2 cut(s) 180, 847
TaiI ACGT 3 cut(s) 250, 327, 518
TaqI TCGA 6 cut(s) 160, 288, 669, 1144, 1253, 1343
TasI AATT 6 cut(s) 150, 293, 307, 315, 963, 1312
TatI WGTACW 1 cut(s) 613
TscAI CASTG 2 cut(s) 424, 778
TseFI GTSAC 1 cut(s) 943
TseI GCWGC 5 cut(s) 251, 539, 620, 1038, 1076
Tsp45I GTSAC 1 cut(s) 943
TspDTI ATGAA 8 cut(s) 95, 201, 306, 480, 618, 1074, 1194, 1204
TspGWI ACGGA 1 cut(s) 23
TspMI CCCGGG 1 cut(s) 543
TspRI CASTG 2 cut(s) 424, 778
VpaK11BI GGWCC 2 cut(s) 788, 917
XapI RAATTY 5 cut(s) 150, 293, 307, 315, 963
XbaI TCTAGA 1 cut(s) 1334
XceI RCATGY 1 cut(s) 223
XhoI CTCGAG 2 cut(s) 668, 1252
XmaI CCCGGG 1 cut(s) 543
XspI CTAG 2 cut(s) 119, 1335
Zsp2I ATGCAT 1 cut(s) 726
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.