AT5G67560
ERF Family

Belongs to the small GTPase superfamily. Arf family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011682
Physical Location & Seq
Forward (+)
26950106 .. 26952285
2180 bp
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UTR
Exon/CDS
Intron
AT5G67560.1

Sequence Viewer

Length: 555 bp
ATGGGTTTGTGGGATGCTCTTCTCAATTGGCTTCGCAGCCTCTTCTTCAAGCAAGAAATGGAGCTTTCATTGATAGGACTTCAAAATGCAGGAAAGACATCACTTGTTAACGTTGTTGCAACCGGTGGATACAGTGAAGACATGATCCCTACGGTTGGTTTCAACATGAGGAAAGTGACAAAAGGAAGTGTCACGATCAAGCTTTGGGATCTCGGTGGTCAACCTAGGTTCCGCAGCATGTGGGAACGTTACTGCCGTTCTGTTTCCGCGATTGTGTATGTAGTTGATGCTGCTGATCCTGATAACCTCAGTGTCTCGAAGAGTGAACTCCATGATTTGTTGAGCAAGACGTCGCTTAATGGTATCCCTCTCCTAGTCCTTGGGAACAAAATCGACAAGCCTGGAGCTTTGTCCAAAGAAGCCCTAACCGATGAAATGGGTCTCAAGTCACTGACAGATAGAGAAGTCTGTTGCTTCATGATATCTTGCAAGAACTCTACCAACATCGATCAAGTCATTGATTGGCTTGTAAAGCACTCCAAATCATCGAATTAA

Protein Analysis

184

Amino Acids

20.4

Weight (kDa)

7.64

Isoelectric Point (pI)

22.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 9 - 178 3.2e-46 ADP-ribosylation factor family
Roc PF08477 22 - 132 9.1e-16 Ras of Complex, Roc, domain of DAPkinase
SRPRB PF09439 22 - 145 3.1e-08 Signal recognition particle receptor beta subunit
Ras PF00071 23 - 179 3.3e-18 Ras family
MMR_HSR1 PF01926 23 - 130 1.4e-06 50S ribosome-binding GTPase
Gtr1_RagA PF04670 24 - 141 5.5e-09 Gtr1/RagA G protein conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011682)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G49860 AT3G49870 AT3G49870 AT5G67560
fragaria_vesca FvH4_1g17760
malus_domestica MD02G1186600.v1.1 MD15G1297800.v1.1
prunus_persica Prupe.6G220000_v2.0.a1
pyrus_communis pycom02g15170
rosa_chinensis RchiOBHm_Chr2g0108091 RchiOBHm_Chr2g0108181
rosa_multiflora Rmu_sc0001484.1_g000004
rosa_roxburghii Rroxscaffold_2G00135640
rosa_rugosa Rorug02G0150100
rosa_samantha Rh2AG199400 Rh2BG212100 Rh2CG204000 Rh2DG207100
rosa_wichuraiana Rw2G015820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 353
AccII CGCG 1 cut(s) 269
AciI CCGC 2 cut(s) 232, 267
AclI AACGTT 2 cut(s) 111, 247
AclWI GGATC 3 cut(s) 139, 216, 290
AcyI GRCGYC 1 cut(s) 350
AfiI CCNNNNNNNGG 1 cut(s) 155
AgeI ACCGGT 1 cut(s) 122
AgsI TTSAA 3 cut(s) 49, 83, 163
AjnI CCWGG 1 cut(s) 400
AluBI AGCT 3 cut(s) 64, 202, 407
AluI AGCT 3 cut(s) 64, 202, 407
Alw26I GTCTC 2 cut(s) 319, 446
AlwI GGATC 3 cut(s) 139, 216, 290
ApeKI GCWGC 3 cut(s) 36, 234, 290
AsiGI ACCGGT 1 cut(s) 122
AspA2I CCTAGG 1 cut(s) 224
AvrII CCTAGG 1 cut(s) 224
BbsI GAAGAC 1 cut(s) 144
BbvI GCAGC 3 cut(s) 48, 246, 277
BceAI ACGGC 1 cut(s) 240
BciT130I CCWGG 1 cut(s) 402
BciVI GTATCC 2 cut(s) 122, 374
BcoDI GTCTC 2 cut(s) 319, 446
BfaI CTAG 2 cut(s) 225, 374
BfuI GTATCC 2 cut(s) 122, 374
BisI GCNGC 3 cut(s) 37, 235, 291
BlnI CCTAGG 1 cut(s) 224
BlsI GCNGC 3 cut(s) 38, 236, 292
Bme1390I CCNGG 1 cut(s) 402
BmiI GGNNCC 1 cut(s) 230
BmrFI CCNGG 1 cut(s) 402
BmsI GCATC 2 cut(s) 4, 277
BpiI GAAGAC 1 cut(s) 144
BpmI CTGGAG 1 cut(s) 423
BpuEI CTTGAG 1 cut(s) 428
Bsa29I ATCGAT 1 cut(s) 507
BsaHI GRCGYC 1 cut(s) 350
BsaI GGTCTC 1 cut(s) 446
BsaJI CCNNGG 2 cut(s) 224, 379
BsaWI WCCGGW 1 cut(s) 122
Bsc4I CCNNNNNNNGG 1 cut(s) 155
Bse118I RCCGGY 1 cut(s) 122
BseBI CCWGG 1 cut(s) 402
BseCI ATCGAT 1 cut(s) 507
BseDI CCNNGG 2 cut(s) 224, 379
BseGI GGATG 1 cut(s) 19
BseLI CCNNNNNNNGG 1 cut(s) 155
BseMII CTCAG 1 cut(s) 322
BseXI GCAGC 3 cut(s) 48, 246, 277
Bsh1236I CGCG 1 cut(s) 269
BshTI ACCGGT 1 cut(s) 122
BshVI ATCGAT 1 cut(s) 507
BsiSI CCGG 1 cut(s) 123
BslI CCNNNNNNNGG 1 cut(s) 155
BsmAI GTCTC 2 cut(s) 319, 446
Bso31I GGTCTC 1 cut(s) 446
Bsp143I GATC 5 cut(s) 144, 195, 208, 295, 508
BspACI CCGC 2 cut(s) 232, 267
BspCNI CTCAG 1 cut(s) 321
BspDI ATCGAT 1 cut(s) 507
BspFNI CGCG 1 cut(s) 269
BspHI TCATGA 1 cut(s) 477
BspLI GGNNCC 1 cut(s) 230
BspPI GGATC 3 cut(s) 139, 216, 290
BspQI GCTCTTC 1 cut(s) 24
BspTNI GGTCTC 1 cut(s) 446
BsrFI RCCGGY 1 cut(s) 122
BssAI RCCGGY 1 cut(s) 122
BssECI CCNNGG 2 cut(s) 224, 379
BssMI GATC 5 cut(s) 144, 195, 208, 295, 508
BssNI GRCGYC 1 cut(s) 350
BssT1I CCWWGG 2 cut(s) 224, 379
Bst2UI CCWGG 1 cut(s) 402
Bst4CI ACNGT 2 cut(s) 134, 154
Bst6I CTCTTC 3 cut(s) 24, 47, 314
BstACI GRCGYC 1 cut(s) 350
BstDEI CTNAG 1 cut(s) 308
BstF5I GGATG 1 cut(s) 19
BstFNI CGCG 1 cut(s) 269
BstKTI GATC 5 cut(s) 147, 198, 211, 298, 511
BstMAI GTCTC 2 cut(s) 319, 446
BstMBI GATC 5 cut(s) 144, 195, 208, 295, 508
BstMWI GCNNNNNNNGC 1 cut(s) 532
BstNI CCWGG 1 cut(s) 402
BstNSI RCATGY 1 cut(s) 241
BstSCI CCNGG 1 cut(s) 400
BstUI CGCG 1 cut(s) 269
BstV1I GCAGC 3 cut(s) 48, 246, 277
BstV2I GAAGAC 1 cut(s) 144
BstX2I RGATCY 1 cut(s) 208
BstYI RGATCY 1 cut(s) 208
Bsu15I ATCGAT 1 cut(s) 507
BsuI GTATCC 2 cut(s) 122, 374
BsuTUI ATCGAT 1 cut(s) 507
BtsCI GGATG 1 cut(s) 19
BtsIMutI CAGTG 3 cut(s) 139, 316, 449
CciI TCATGA 1 cut(s) 477
Cfr10I RCCGGY 1 cut(s) 122
ClaI ATCGAT 1 cut(s) 507
CspAI ACCGGT 1 cut(s) 122
CviAII CATG 5 cut(s) 142, 166, 238, 332, 478
CviJI RGCY 8 cut(s) 31, 39, 64, 202, 400, 407, 422, 526
CviKI_1 RGCY 8 cut(s) 31, 39, 64, 202, 400, 407, 422, 526
DdeI CTNAG 1 cut(s) 308
DpnI GATC 5 cut(s) 146, 197, 210, 297, 510
DpnII GATC 5 cut(s) 144, 195, 208, 295, 508
Eam1104I CTCTTC 3 cut(s) 24, 47, 314
EarI CTCTTC 3 cut(s) 24, 47, 314
Eco130I CCWWGG 2 cut(s) 224, 379
Eco31I GGTCTC 1 cut(s) 446
Eco32I GATATC 1 cut(s) 483
EcoRII CCWGG 1 cut(s) 400
EcoRV GATATC 1 cut(s) 483
EcoT14I CCWWGG 2 cut(s) 224, 379
ErhI CCWWGG 2 cut(s) 224, 379
FaeI CATG 5 cut(s) 145, 169, 241, 335, 481
FaiI YATR 6 cut(s) 143, 167, 239, 279, 333, 479
FatI CATG 5 cut(s) 141, 165, 237, 331, 477
Fnu4HI GCNGC 3 cut(s) 37, 235, 291
FokI GGATG 1 cut(s) 26
Fsp4HI GCNGC 3 cut(s) 37, 235, 291
FspBI CTAG 2 cut(s) 225, 374
GluI GCNGC 3 cut(s) 37, 235, 291
GsuI CTGGAG 1 cut(s) 423
HapII CCGG 1 cut(s) 123
Hin1I GRCGYC 1 cut(s) 350
Hin1II CATG 5 cut(s) 145, 169, 241, 335, 481
HincII GTYRAC 2 cut(s) 109, 221
HindII GTYRAC 2 cut(s) 109, 221
HindIII AAGCTT 1 cut(s) 200
HpaI GTTAAC 1 cut(s) 109
HpaII CCGG 1 cut(s) 123
Hpy166II GTNNAC 3 cut(s) 109, 221, 326
Hpy188III TCNNGA 4 cut(s) 193, 299, 316, 478
Hpy8I GTNNAC 3 cut(s) 109, 221, 326
Hpy99I CGWCG 1 cut(s) 355
HpyCH4III ACNGT 2 cut(s) 134, 154
HpyCH4IV ACGT 3 cut(s) 111, 247, 350
HpyCH4V TGCA 3 cut(s) 89, 119, 489
HpyF10VI GCNNNNNNNGC 1 cut(s) 532
HpyF3I CTNAG 1 cut(s) 308
HpySE526I ACGT 3 cut(s) 111, 247, 350
Hsp92I GRCGYC 1 cut(s) 350
Hsp92II CATG 5 cut(s) 145, 169, 241, 335, 481
KspAI GTTAAC 1 cut(s) 109
Kzo9I GATC 5 cut(s) 144, 195, 208, 295, 508
LguI GCTCTTC 1 cut(s) 24
LmnI GCTCC 2 cut(s) 61, 404
LpnPI CCDG 5 cut(s) 75, 136, 312, 387, 414
Lsp1109I GCAGC 3 cut(s) 48, 246, 277
LweI GCATC 2 cut(s) 4, 277
MaeI CTAG 2 cut(s) 225, 374
MaeII ACGT 3 cut(s) 111, 247, 350
MaeIII GTNAC 4 cut(s) 175, 190, 248, 447
MalI GATC 5 cut(s) 146, 197, 210, 297, 510
MboI GATC 5 cut(s) 144, 195, 208, 295, 508
MboII GAAGA 5 cut(s) 11, 34, 37, 149, 331
MfeI CAATTG 1 cut(s) 25
MflI RGATCY 1 cut(s) 208
MluCI AATT 2 cut(s) 25, 550
MnlI CCTC 4 cut(s) 50, 162, 317, 378
MseI TTAA 3 cut(s) 108, 357, 553
MspI CCGG 1 cut(s) 123
MspR9I CCNGG 1 cut(s) 402
MunI CAATTG 1 cut(s) 25
MvaI CCWGG 1 cut(s) 402
MvnI CGCG 1 cut(s) 269
MwoI GCNNNNNNNGC 1 cut(s) 532
NdeII GATC 5 cut(s) 144, 195, 208, 295, 508
NlaIII CATG 5 cut(s) 145, 169, 241, 335, 481
NlaIV GGNNCC 1 cut(s) 230
NmuCI GTSAC 3 cut(s) 175, 190, 447
NspI RCATGY 1 cut(s) 241
PagI TCATGA 1 cut(s) 477
PciSI GCTCTTC 1 cut(s) 24
PcsI WCGNNNNNNNCGW 1 cut(s) 253
PinAI ACCGGT 1 cut(s) 122
PkrI GCNGC 3 cut(s) 38, 236, 292
Psp1406I AACGTT 2 cut(s) 111, 247
Psp6I CCWGG 1 cut(s) 400
PspGI CCWGG 1 cut(s) 400
PspN4I GGNNCC 1 cut(s) 230
PsuI RGATCY 1 cut(s) 208
SapI GCTCTTC 1 cut(s) 24
SaqAI TTAA 3 cut(s) 108, 357, 553
SatI GCNGC 3 cut(s) 37, 235, 291
Sau3AI GATC 5 cut(s) 144, 195, 208, 295, 508
ScrFI CCNGG 1 cut(s) 402
SetI ASST 9 cut(s) 66, 114, 204, 226, 230, 250, 309, 353, 409
SfaNI GCATC 2 cut(s) 4, 277
SmlI CTYRAG 1 cut(s) 443
SmoI CTYRAG 1 cut(s) 443
Sse9I AATT 2 cut(s) 25, 550
SsiI CCGC 2 cut(s) 232, 267
SspMI CTAG 2 cut(s) 225, 374
StyD4I CCNGG 1 cut(s) 400
StyI CCWWGG 2 cut(s) 224, 379
TaaI ACNGT 2 cut(s) 134, 154
TaiI ACGT 3 cut(s) 114, 250, 353
TaqI TCGA 4 cut(s) 317, 393, 507, 548
TasI AATT 2 cut(s) 25, 550
Tru1I TTAA 3 cut(s) 108, 357, 553
Tru9I TTAA 3 cut(s) 108, 357, 553
TscAI CASTG 3 cut(s) 139, 316, 456
TseFI GTSAC 3 cut(s) 175, 190, 447
TseI GCWGC 3 cut(s) 36, 234, 290
Tsp45I GTSAC 3 cut(s) 175, 190, 447
TspDTI ATGAA 3 cut(s) 57, 447, 466
TspRI CASTG 3 cut(s) 139, 316, 456
XceI RCATGY 1 cut(s) 241
XmaJI CCTAGG 1 cut(s) 224
XspI CTAG 2 cut(s) 225, 374
ZraI GACGTC 1 cut(s) 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.