Rh2CG204000
ERF Family

Belongs to the small GTPase superfamily. Arf family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0011682
Physical Location & Seq
Forward (+)
19865451 .. 19869045
3595 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG204000.1

Sequence Viewer

Length: 498 bp
ATGGAGCTATCTTTGATAGGACTTCAGAATGCTGGAAAGACTTCTCTTGTAAATGTTGTTGCAACGGGAGGATACAGTGAGGACATGATCCCTACGGTAGGATTCAATATGAAGAAGGTAACCAAAGGGAATGTTACAATAAAGTTGTGGGATCTTGGAGGTCAACCCAGGTTTCGTAGCATGTGGGAGCGATACTGTCGTGCGGTTTCTGCTATAGTCTATGTTGTTGATGCTGCTGATCCTGATAACTTGAGCACCTCAAGAGGGGAACTTCATGATTTGCTTAGCAAATCCTCACTTAATGGTATCCCATTGCTGATCCTGGGTAACAAGATTGATAAACCAGGAGCTCTGAGTAAACAAGCTTTGACTGATGAAATGGGACTCAAGTCAATTACTGATAGAGAAGTATGTTGCTTCATGATCTCATGCAAGAACTCAACCAACATAGACTCAGTTATTGATTGGCTTGTAAAGCATTCCAAATCAAAAAGCTGA

Protein Analysis

165

Amino Acids

18.01

Weight (kDa)

8.67

Isoelectric Point (pI)

17.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Roc PF08477 3 - 113 4.2e-16 Ras of Complex, Roc, domain of DAPkinase
Arf PF00025 4 - 159 7.7e-45 ADP-ribosylation factor family
Ras PF00071 4 - 160 2.5e-18 Ras family
MMR_HSR1 PF01926 4 - 111 1e-06 50S ribosome-binding GTPase
Gtr1_RagA PF04670 5 - 121 1.8e-09 Gtr1/RagA G protein conserved region
SRPRB PF09439 5 - 127 5.9e-09 Signal recognition particle receptor beta subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011682)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G49860 AT3G49870 AT3G49870 AT5G67560
fragaria_vesca FvH4_1g17760
malus_domestica MD02G1186600.v1.1 MD15G1297800.v1.1
prunus_persica Prupe.6G220000_v2.0.a1
pyrus_communis pycom02g15170
rosa_chinensis RchiOBHm_Chr2g0108091 RchiOBHm_Chr2g0108181
rosa_multiflora Rmu_sc0001484.1_g000004
rosa_roxburghii Rroxscaffold_2G00135640
rosa_rugosa Rorug02G0150100
rosa_samantha Rh2AG199400 Rh2BG212100 Rh2CG204000 Rh2DG207100
rosa_wichuraiana Rw2G015820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 203
AclWI GGATC 4 cut(s) 82, 159, 233, 313
AcuI CTGAAG 1 cut(s) 8
AfiI CCNNNNNNNGG 2 cut(s) 98, 264
AgsI TTSAA 1 cut(s) 106
AjnI CCWGG 3 cut(s) 167, 321, 343
AluBI AGCT 4 cut(s) 7, 350, 365, 495
AluI AGCT 4 cut(s) 7, 350, 365, 495
Alw21I GWGCWC 2 cut(s) 257, 352
AlwI GGATC 4 cut(s) 82, 159, 233, 313
ApeKI GCWGC 1 cut(s) 233
Asp700I GAANNNNTTC 1 cut(s) 40
BaeI ACNNNNGTAYC 2 cut(s) 184, 217
BanII GRGCYC 1 cut(s) 352
Bbv12I GWGCWC 2 cut(s) 257, 352
BbvI GCAGC 1 cut(s) 220
BciT130I CCWGG 3 cut(s) 169, 323, 345
BciVI GTATCC 2 cut(s) 65, 317
BfmI CTRYAG 1 cut(s) 213
BfuI GTATCC 2 cut(s) 65, 317
BisI GCNGC 1 cut(s) 234
BlpI GCTNAGC 1 cut(s) 284
BlsI GCNGC 1 cut(s) 235
Bme1390I CCNGG 3 cut(s) 169, 323, 345
BmrFI CCNGG 3 cut(s) 169, 323, 345
BmsI GCATC 1 cut(s) 220
BoxI GACNNNNGTC 1 cut(s) 388
Bpu1102I GCTNAGC 1 cut(s) 284
BpuEI CTTGAG 3 cut(s) 244, 271, 371
BsaJI CCNNGG 2 cut(s) 167, 322
Bsc4I CCNNNNNNNGG 2 cut(s) 98, 264
Bse3DI GCAATG 1 cut(s) 311
BseBI CCWGG 3 cut(s) 169, 323, 345
BseDI CCNNGG 2 cut(s) 167, 322
BseLI CCNNNNNNNGG 2 cut(s) 98, 264
BseMI GCAATG 1 cut(s) 311
BseMII CTCAG 2 cut(s) 344, 468
BseXI GCAGC 1 cut(s) 220
BsiHKAI GWGCWC 2 cut(s) 257, 352
BslFI GGGAC 1 cut(s) 396
BslI CCNNNNNNNGG 2 cut(s) 98, 264
BsmFI GGGAC 1 cut(s) 396
BsmI GAATGC 2 cut(s) 34, 478
Bsp1286I GDGCHC 2 cut(s) 257, 352
Bsp143I GATC 5 cut(s) 87, 151, 238, 318, 423
Bsp1720I GCTNAGC 1 cut(s) 284
BspACI CCGC 1 cut(s) 203
BspCNI CTCAG 2 cut(s) 345, 467
BspHI TCATGA 2 cut(s) 274, 420
BspPI GGATC 4 cut(s) 82, 159, 233, 313
BsrDI GCAATG 1 cut(s) 311
BssECI CCNNGG 2 cut(s) 167, 322
BssMI GATC 5 cut(s) 87, 151, 238, 318, 423
Bst2UI CCWGG 3 cut(s) 169, 323, 345
Bst4CI ACNGT 3 cut(s) 77, 97, 197
BstDEI CTNAG 3 cut(s) 284, 353, 454
BstEII GGTNACC 1 cut(s) 118
BstENI CCTNNNNNAGG 1 cut(s) 96
BstKTI GATC 5 cut(s) 90, 154, 241, 321, 426
BstMBI GATC 5 cut(s) 87, 151, 238, 318, 423
BstMWI GCNNNNNNNGC 2 cut(s) 209, 475
BstNI CCWGG 3 cut(s) 169, 323, 345
BstNSI RCATGY 1 cut(s) 184
BstPAI GACNNNNGTC 1 cut(s) 388
BstPI GGTNACC 1 cut(s) 118
BstSCI CCNGG 3 cut(s) 167, 321, 343
BstSFI CTRYAG 1 cut(s) 213
BstV1I GCAGC 1 cut(s) 220
BstX2I RGATCY 1 cut(s) 151
BstYI RGATCY 1 cut(s) 151
BsuI GTATCC 2 cut(s) 65, 317
BtsIMutI CAGTG 1 cut(s) 82
CciI TCATGA 2 cut(s) 274, 420
CviAII CATG 5 cut(s) 85, 181, 275, 421, 429
CviJI RGCY 5 cut(s) 7, 350, 365, 469, 495
CviKI_1 RGCY 5 cut(s) 7, 350, 365, 469, 495
DdeI CTNAG 3 cut(s) 284, 353, 454
DpnI GATC 5 cut(s) 89, 153, 240, 320, 425
DpnII GATC 5 cut(s) 87, 151, 238, 318, 423
Ecl136II GAGCTC 1 cut(s) 350
Eco24I GRGCYC 1 cut(s) 352
Eco53kI GAGCTC 1 cut(s) 350
Eco57I CTGAAG 1 cut(s) 8
Eco91I GGTNACC 1 cut(s) 118
EcoICRI GAGCTC 1 cut(s) 350
EcoNI CCTNNNNNAGG 1 cut(s) 96
EcoO65I GGTNACC 1 cut(s) 118
EcoRII CCWGG 3 cut(s) 167, 321, 343
EcoT38I GRGCYC 1 cut(s) 352
FaeI CATG 5 cut(s) 88, 184, 278, 424, 432
FaqI GGGAC 1 cut(s) 396
FatI CATG 5 cut(s) 84, 180, 274, 420, 428
Fnu4HI GCNGC 1 cut(s) 234
FriOI GRGCYC 1 cut(s) 352
Fsp4HI GCNGC 1 cut(s) 234
GluI GCNGC 1 cut(s) 234
Hin1II CATG 5 cut(s) 88, 184, 278, 424, 432
HincII GTYRAC 1 cut(s) 164
HindII GTYRAC 1 cut(s) 164
HindIII AAGCTT 1 cut(s) 363
HinfI GANTC 3 cut(s) 102, 384, 452
Hpy166II GTNNAC 2 cut(s) 164, 359
Hpy188I TCNGA 2 cut(s) 27, 354
Hpy188III TCNNGA 4 cut(s) 242, 261, 275, 421
Hpy8I GTNNAC 2 cut(s) 164, 359
HpyAV CCTTC 1 cut(s) 109
HpyCH4III ACNGT 3 cut(s) 77, 97, 197
HpyCH4V TGCA 2 cut(s) 62, 432
HpyF10VI GCNNNNNNNGC 2 cut(s) 209, 475
HpyF3I CTNAG 3 cut(s) 284, 353, 454
Hsp92II CATG 5 cut(s) 88, 184, 278, 424, 432
Kzo9I GATC 5 cut(s) 87, 151, 238, 318, 423
LmnI GCTCC 3 cut(s) 4, 187, 347
LpnPI CCDG 8 cut(s) 18, 154, 181, 255, 308, 330, 335, 357
Lsp1109I GCAGC 1 cut(s) 220
LweI GCATC 1 cut(s) 220
MaeIII GTNAC 3 cut(s) 118, 133, 326
MalI GATC 5 cut(s) 89, 153, 240, 320, 425
MboI GATC 5 cut(s) 87, 151, 238, 318, 423
MboII GAAGA 1 cut(s) 124
MflI RGATCY 1 cut(s) 151
MhlI GDGCHC 2 cut(s) 257, 352
MluCI AATT 1 cut(s) 393
MlyI GAGTC 2 cut(s) 378, 446
MnlI CCTC 6 cut(s) 62, 73, 152, 257, 268, 304
MroXI GAANNNNTTC 1 cut(s) 40
MseI TTAA 1 cut(s) 300
MspR9I CCNGG 3 cut(s) 169, 323, 345
Mva1269I GAATGC 2 cut(s) 34, 478
MvaI CCWGG 3 cut(s) 169, 323, 345
MwoI GCNNNNNNNGC 2 cut(s) 209, 475
NdeII GATC 5 cut(s) 87, 151, 238, 318, 423
NlaIII CATG 5 cut(s) 88, 184, 278, 424, 432
NspI RCATGY 1 cut(s) 184
PagI TCATGA 2 cut(s) 274, 420
PctI GAATGC 2 cut(s) 34, 478
PdmI GAANNNNTTC 1 cut(s) 40
PfeI GAWTC 1 cut(s) 102
PkrI GCNGC 1 cut(s) 235
PleI GAGTC 2 cut(s) 378, 446
PpsI GAGTC 2 cut(s) 378, 446
PshAI GACNNNNGTC 1 cut(s) 388
Psp124BI GAGCTC 1 cut(s) 352
Psp6I CCWGG 3 cut(s) 167, 321, 343
PspEI GGTNACC 1 cut(s) 118
PspGI CCWGG 3 cut(s) 167, 321, 343
PsuI RGATCY 1 cut(s) 151
SacI GAGCTC 1 cut(s) 352
SaqAI TTAA 1 cut(s) 300
SatI GCNGC 1 cut(s) 234
Sau3AI GATC 5 cut(s) 87, 151, 238, 318, 423
SchI GAGTC 2 cut(s) 378, 446
ScrFI CCNGG 3 cut(s) 169, 323, 345
SduI GDGCHC 2 cut(s) 257, 352
SetI ASST 8 cut(s) 9, 120, 163, 173, 260, 352, 367, 497
SfaNI GCATC 1 cut(s) 220
SfcI CTRYAG 1 cut(s) 213
SmlI CTYRAG 3 cut(s) 250, 259, 386
SmoI CTYRAG 3 cut(s) 250, 259, 386
Sse9I AATT 1 cut(s) 393
SsiI CCGC 1 cut(s) 203
SstI GAGCTC 1 cut(s) 352
StyD4I CCNGG 3 cut(s) 167, 321, 343
TaaI ACNGT 3 cut(s) 77, 97, 197
TasI AATT 1 cut(s) 393
TfiI GAWTC 1 cut(s) 102
Tru1I TTAA 1 cut(s) 300
Tru9I TTAA 1 cut(s) 300
TscAI CASTG 1 cut(s) 82
TseI GCWGC 1 cut(s) 233
TspDTI ATGAA 4 cut(s) 125, 263, 390, 409
TspRI CASTG 1 cut(s) 82
XagI CCTNNNNNAGG 1 cut(s) 96
XceI RCATGY 1 cut(s) 184
XmnI GAANNNNTTC 1 cut(s) 40
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.