Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0014543

Physical Location & Seq

Genomic Coordinates Reverse (-)

799489 .. 803803

Gene Structure 4315 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g01520.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2859 bp

ATGGAGGTTTCCCATTCCTTCTTCTCCCACTCGAATCTTCTTGTCTTGTTCATGTTCTTTGCAGCTGTTTCAGCAGCTGATCTTTACTCACAGACCCTTCTGAGCTTAAAATCTGAGCTCACTGATGACTATGATACCTTAAAAGATTGGTATGTGCCTCCTGGAGAAAACCCATCTCCAAAAATCTATGCTTGTTCTTGGTCTGGTGTCAAATGCAACAACAACTCTGTTACTGGTCTAGACCTCTCAATGAAGAGACTTTCAGGTTCAATCTCCGGCGACCGAGTCAACTTCAGTGTCTTCACCGATCTTCTTGATCTCAACCTGAGTTACAACTCATTTTCCGGCAAGCTCCCAGTGTCAATGTTCACCCTGGCCAACCTCACAAGCTTGGACATCAGCCGGAACAACTTCTCCGGCCAGTTCCCAGCTGGAGTCTCCGGTCTTAAAAATCTGGTTGTGTTTGATGCATTCAGCAACAGCTTTTCTGGGTCCCTGCCTGTTGAAGTGTCTCAACTTCAGAATCTCAAGATTCTCAATTTAGCTGGGAGCTACTTCAAGGGTCCAATCCCACAAGAATATGGTAATTTTCAAAGCCTTCAGTTTATTCATCTTGCAGGGAATATGCTCAATGGGAACATACCACCTGAATTGGGTAAGCTCAAAACAGTGACCCATATGGAGATTGGCTATAATACTTACCAGGGAGGCATCCCTTGGCAGTTGGGCAACATGAGCCAGCTTCAGTATCTTGATATTGCTTATGCAAATCTCTCTGAGTCTATTCCAAGAGAGCTTGGGAATCTCACCAAGCTTGAGTCTCTTTTTCTCTTCAGAAACCAGCTCAGTGGATCAATACCAGTGGACTTCAGTAAGCTTCAATCTCTCATAAGCTTGGATCTTTCAGATAATCTGATTTCCGGGCCGATCTCAGATAGTCTTCTAGAGTTGAAGAACCTGAAACTGTTGAGTCTTTTTTACAATGAGATGAGTGGTGTTGTTCCTGAAGGAATTGCTGAGCTTCCATCACTTGAGTCTCTTCTTATATGGAACAACTACTTCTCTGGGAATCTTCCACAGAATTTGGGGAGGAACTCTAATCTTATGTGGGTTGATGTGTCTACAAACAACTTCAATGGCAGCATTCCACCAGATATATGTGTCAAAGGTGTGTTGTTTAAGCTGATGCTGTTTTCGAATAACTTTAGTGGCAGTCTTGCACCATCTCTCTCCAATTGTTCTTCGCTTGTTCGTCTTCGGCTTGAAGATAACTCATTCTCAGGTGAGATCCCTTTGAGATTCAGCCATCTTCGTGATCTTTCATATGTAGATTTGTCCAAGAATAAACTTACTGGTGGGATTCCAATAGATATAGACCAAGCTCCCAAGCTTGAGTACTTTAATATGTCCAATAATCCTGAATTAGGGGGCACAATTCCTGCAGTAACATGGTCTTTGCCACTTCTTCAGAATTTCTCAGCTTCCTCGTGTAGTATCTTAGGCAATCTTCCTCCATTCGGAAGCTGTAAATCCATATCTGTCATTGAATTGAACATGAACAGTATATCAGGGGCTATTTCAGAAAGTGTTTCTAACTGCCTAGCCCTTGAGAGAATTGATTTAGCCAAAAATAACTTATCAGGTCATATACCTGAGGAGCTTGCAAGTCTTCCTGCTCTGCGTGTCCTAGACCTGTCACATAATAGTTTAAATGGACCAATACCTTCAAAGTTTGGTTCTTCTTCAAGCTTATCACTTCTAAATGTGTCTTTCAATGATATATCTGGGACAATTCCCTCTGGAAAGGTAGTGAGAACTATGGGAAGTAGCGCATTTGTGGGAAATTCAAGGCTATGTGGAGAACCTTTGAGATCATGTCCAGGTTCAGTGGCTATCTTCGGAAGCAGAGGCATTGGGAAGCTTATATGGGTCTTGCTACTGTGTGCAGGCATAGTTATGTTCCTTACTCTGTCAGTTCTAGGAGTACTTTATTTTCAAAAGGGCAGCAGAGGCCAGTGGAAGATGGTCTCATTTTCTGGACTTCCTCAATTCACGGCAAAAGATGTATTGAAGAGCTTTAATTCCATAGAATCCATGGACGATACATTGCCTCCAATATCAGCTTCAGTATGCAAAGCAGTTCTGCCAACAGGAATCACAGTTTCAGTGAAGAAGCTTGAATGGGATCCAAATATAGCAAAAGATATGCTGCCATTCATAACCCAAATTGGTAACGCAAGGCACAAGAATCTAATCCGATTGCTCGGATTTTGCTACAACAAGCATCTGGTATATCTCTTGTACGATTACTTGCCCAATGGAAGTTTATCAGAAAATATTAGTGTGAAAAGGGAATGGGCAGCTAAGTACAAAATTGTGGTGAGCATTGCAAGGGGATTGTGCTTCCTTCACCATGAATGTCATCCTGCAATTGCTCATGGAGACTTGAGATCAAGTAATATAGTGTTTGATGAAAACATGGAACCACATCTGACAGAATTTGGACTCAAGCATCTGCTCGAGTTGAACAAAACTTCAGGTACCGCAGAAACTCCCGCAATGTATACAGGTGCCACAAACAGTTCTGCTGAAGAACTATACAGAGATGTATACAGCTTCGGGGAGATCATGCTCGAAATCTTAACTAATGGCAAGTTGACGAATTCAGGAGCATCCTTACACAACAAGTCAAGGGAAATTGTTCTCAGAGATATAATCAATGAGCACGAAGTTGGTTCCAACACTTTAGTGCAGGAGGAGATAAAATGGGTGCTCGAGGTTGCTACACTCTGCACTAGGATCCGATCATCAGACCGACCATCCATGAAGGATACATTAAAGCTTTTACAGGCTCCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001763 GO:0003006 GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009987 GO:0010016 GO:0010065 GO:0010067 GO:0010087 GO:0010089 GO:0010154 GO:0010223 GO:0010346 GO:0016020 GO:0022414 GO:0032501 GO:0032502 GO:0044464 GO:0048316 GO:0048367 GO:0048507 GO:0048508 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0051301 GO:0061458 GO:0071944 GO:1905393

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

953

Amino Acids

104.23

Weight (kDa)

5.99

Isoelectric Point (pI)

34.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_14	PF23598	169 - 277	2.4e-10	Leucine-rich repeat region
LRR_8	PF13855	513 - 570	1.5e-07	Leucine rich repeat
LRR_8	PF13855	536 - 594	7.7e-07	Leucine rich repeat
Pkinase	PF00069	709 - 946	4e-18	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	717 - 947	1.4e-21	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0014543)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G51350
fragaria_vesca	FvH4_1g01520
malus_domestica	MD02G1012000.v1.1 MD15G1157800.v1.1
prunus_persica	Prupe.7G253000_v2.0.a1
pyrus_communis	pycom02g01130 pycom15g14160
rosa_chinensis	RchiOBHm_Chr2g0086441
rosa_laevigata	RLG00000015759
rosa_multiflora	Rmu_sc0005127.1_g000003
rosa_roxburghii	Rroxscaffold_2G00154570
rosa_rugosa	Rorug01G0467300
rosa_samantha	Rh2AG018100 Rh2BG017600 Rh2CG018500 Rh2DG019400
rosa_wichuraiana	Rw2G001500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	2540
AccB1I	GGYRCC	2 cut(s)	2540, 2570
AccI	GTMKAC	3 cut(s)	1121, 2564, 2610
AciI	CCGC	2 cut(s)	2544, 2556
AclWI	GGATC	7 cut(s)	859, 906, 1282, 2180, 2193, 2794, 2807
AcoI	YGGCCR	2 cut(s)	375, 418
AcsI	RAATTY	5 cut(s)	1081, 1471, 1843, 2498, 2662
AfaI	GTAC	5 cut(s)	1397, 1986, 2303, 2369, 2542
AfiI	CCNNNNNNNGG	3 cut(s)	653, 1424, 1517
AjnI	CCWGG	4 cut(s)	160, 372, 702, 1879
AjuI	GAANNNNNNNTTGG	2 cut(s)	2107, 2139
AleI	CACNNNNGTG	1 cut(s)	2747
AloI	GAACNNNNNNTCC	2 cut(s)	398, 430
Alw21I	GWGCWC	3 cut(s)	120, 2727, 2775
Alw26I	GTCTC	7 cut(s)	250, 442, 516, 825, 1041, 2032, 2436
AlwI	GGATC	7 cut(s)	859, 906, 1282, 2180, 2193, 2794, 2807
AlwNI	CAGNNNCTG	1 cut(s)	77
Ama87I	CYCGRG	2 cut(s)	2519, 2774
AoxI	GGCC	4 cut(s)	375, 418, 923, 2011
ApeKI	GCWGC	6 cut(s)	62, 74, 1140, 2004, 2209, 2360
ApoI	RAATTY	5 cut(s)	1081, 1471, 1843, 2498, 2662
Asp700I	GAANNNNTTC	2 cut(s)	410, 2700
Asp718I	GGTACC	1 cut(s)	2540
AspLEI	GCGC	1 cut(s)	1832
AspS9I	GGNCC	4 cut(s)	492, 563, 923, 1715
AsuC2I	CCSGG	1 cut(s)	922
AsuHPI	GGTGA	6 cut(s)	295, 361, 799, 1295, 2392, 2402
AsuII	TTCGAA	1 cut(s)	1196
AvaI	CYCGRG	2 cut(s)	2519, 2774
AvaII	GGWCC	3 cut(s)	492, 563, 1715
AxyI	CCTNAGG	1 cut(s)	1653
BaeGI	GKGCMC	1 cut(s)	1433
BalI	TGGCCA	1 cut(s)	377
BamHI	GGATCC	2 cut(s)	2185, 2799
BanI	GGYRCC	2 cut(s)	2540, 2570
BanII	GRGCYC	1 cut(s)	120
BauI	CACGAG	1 cut(s)	1486
BbsI	GAAGAC	4 cut(s)	292, 932, 1247, 1661
Bbv12I	GWGCWC	3 cut(s)	120, 2727, 2775
BbvI	GCAGC	6 cut(s)	74, 86, 1152, 2016, 2196, 2372
BccI	CCATC	6 cut(s)	181, 1033, 1231, 1314, 2017, 2827
BceAI	ACGGC	1 cut(s)	2070
BciT130I	CCWGG	4 cut(s)	162, 374, 704, 1881
BciVI	GTATCC	1 cut(s)	2824
BcnI	CCSGG	1 cut(s)	922
BcoDI	GTCTC	7 cut(s)	250, 442, 516, 825, 1041, 2032, 2436
BfaI	CTAG	6 cut(s)	239, 944, 1601, 1688, 1979, 2796
BfmI	CTRYAG	1 cut(s)	1440
BfuI	GTATCC	1 cut(s)	2824
BisI	GCNGC	6 cut(s)	63, 75, 1141, 2005, 2210, 2361
BlpI	GCTNAGC	1 cut(s)	1017
BlsI	GCNGC	6 cut(s)	64, 76, 1142, 2006, 2211, 2362
BmcAI	AGTACT	2 cut(s)	1397, 1986
Bme1390I	CCNGG	5 cut(s)	162, 374, 704, 922, 1881
Bme18I	GGWCC	3 cut(s)	492, 563, 1715
BmeT110I	CYCGRG	2 cut(s)	2519, 2774
BmgT120I	GGNCC	4 cut(s)	492, 563, 923, 1715
BmrFI	CCNGG	5 cut(s)	162, 374, 704, 922, 1881
BmrI	ACTGGG	1 cut(s)	350
BmsI	GCATC	6 cut(s)	457, 720, 1176, 2293, 2521, 2681
BmuI	ACTGGG	1 cut(s)	350
BpiI	GAAGAC	4 cut(s)	292, 932, 1247, 1661
BpmI	CTGGAG	2 cut(s)	183, 453
Bpu1102I	GCTNAGC	1 cut(s)	1017
Bpu14I	TTCGAA	1 cut(s)	1196
BpuEI	CTTGAG	7 cut(s)	512, 836, 1052, 1412, 1628, 2467, 2492
BpuMI	CCSGG	1 cut(s)	922
BsaI	GGTCTC	1 cut(s)	2032
BsaJI	CCNNGG	4 cut(s)	372, 703, 716, 2094
BsaWI	WCCGGW	1 cut(s)	440
BsaXI	ACNNNNNCTCC	4 cut(s)	398, 428, 2095, 2125
Bsc4I	CCNNNNNNNGG	3 cut(s)	653, 1424, 1517
Bse1I	ACTGG	6 cut(s)	238, 356, 421, 860, 1357, 2014
Bse21I	CCTNAGG	1 cut(s)	1653
Bse3DI	GCAATG	3 cut(s)	2105, 2385, 2565
BseBI	CCWGG	4 cut(s)	162, 374, 704, 1881
BseDI	CCNNGG	4 cut(s)	372, 703, 716, 2094
BseGI	GGATG	4 cut(s)	711, 2422, 2672, 2819
BseLI	CCNNNNNNNGG	3 cut(s)	653, 1424, 1517
BseMI	GCAATG	3 cut(s)	2105, 2385, 2565
BseNI	ACTGG	6 cut(s)	238, 356, 421, 860, 1357, 2014
BseRI	GAGGAG	2 cut(s)	1670, 2771
BseSI	GKGCMC	1 cut(s)	1433
BseXI	GCAGC	6 cut(s)	74, 86, 1152, 2016, 2196, 2372
BseYI	CCCAGC	2 cut(s)	427, 545
BsgI	GTGCAG	3 cut(s)	1965, 2771, 2776
Bsh1285I	CGRYCG	1 cut(s)	283
BshFI	GGCC	4 cut(s)	377, 420, 925, 2013
BshNI	GGYRCC	2 cut(s)	2540, 2570
BsiEI	CGRYCG	1 cut(s)	283
BsiHKAI	GWGCWC	3 cut(s)	120, 2727, 2775
BsiHKCI	CYCGRG	2 cut(s)	2519, 2774
BsiSI	CCGG	6 cut(s)	276, 345, 403, 417, 441, 921
BslFI	GGGAC	2 cut(s)	478, 1801
BslI	CCNNNNNNNGG	3 cut(s)	653, 1424, 1517
BsmAI	GTCTC	7 cut(s)	250, 442, 516, 825, 1041, 2032, 2436
BsmFI	GGGAC	2 cut(s)	478, 1801
BsmI	GAATGC	2 cut(s)	470, 1143
BsnI	GGCC	4 cut(s)	377, 420, 925, 2013
Bso31I	GGTCTC	1 cut(s)	2032
BsoBI	CYCGRG	2 cut(s)	2519, 2774
Bsp119I	TTCGAA	1 cut(s)	1196
Bsp1286I	GDGCHC	4 cut(s)	120, 1433, 2727, 2775
Bsp1720I	GCTNAGC	1 cut(s)	1017
Bsp19I	CCATGG	1 cut(s)	2094
BspACI	CCGC	2 cut(s)	2544, 2556
BspANI	GGCC	4 cut(s)	377, 420, 925, 2013
BspMAI	CTGCAG	1 cut(s)	1444
BspPI	GGATC	7 cut(s)	859, 906, 1282, 2180, 2193, 2794, 2807
BspQI	GCTCTTC	1 cut(s)	2066
BspT104I	TTCGAA	1 cut(s)	1196
BspT107I	GGYRCC	2 cut(s)	2540, 2570
BspTNI	GGTCTC	1 cut(s)	2032
BsrDI	GCAATG	3 cut(s)	2105, 2385, 2565
BsrI	ACTGG	6 cut(s)	238, 356, 421, 860, 1357, 2014
BssECI	CCNNGG	4 cut(s)	372, 703, 716, 2094
BssNAI	GTATAC	2 cut(s)	2565, 2611
BssSI	CACGAG	1 cut(s)	1486
BssT1I	CCWWGG	2 cut(s)	716, 2094
Bst1107I	GTATAC	2 cut(s)	2565, 2611
Bst2BI	CACGAG	1 cut(s)	1486
Bst2UI	CCWGG	4 cut(s)	162, 374, 704, 1881
Bst4CI	ACNGT	6 cut(s)	670, 966, 1562, 1941, 2161, 2582
Bst6I	CTCTTC	4 cut(s)	248, 836, 1044, 2066
BstBI	TTCGAA	1 cut(s)	1196
BstC8I	GCNNGC	4 cut(s)	350, 740, 1662, 1948
BstDSI	CCRYGG	1 cut(s)	2094
BstENI	CCTNNNNNAGG	1 cut(s)	1422
BstF5I	GGATG	4 cut(s)	711, 2422, 2672, 2819
BstHHI	GCGC	1 cut(s)	1832
BstMAI	GTCTC	7 cut(s)	250, 442, 516, 825, 1041, 2032, 2436
BstMCI	CGRYCG	1 cut(s)	283
BstMWI	GCNNNNNNNGC	3 cut(s)	71, 735, 2010
BstNI	CCWGG	4 cut(s)	162, 374, 704, 1881
BstSCI	CCNGG	5 cut(s)	160, 372, 702, 920, 1879
BstSFI	CTRYAG	1 cut(s)	1440
BstSLI	GKGCMC	1 cut(s)	1433
BstV1I	GCAGC	6 cut(s)	74, 86, 1152, 2016, 2196, 2372
BstV2I	GAAGAC	4 cut(s)	292, 932, 1247, 1661
BstX2I	RGATCY	4 cut(s)	898, 1287, 2185, 2799
BstXI	CCANNNNNNTGG	1 cut(s)	848
BstYI	RGATCY	4 cut(s)	898, 1287, 2185, 2799
BstZ17I	GTATAC	2 cut(s)	2565, 2611
Bsu36I	CCTNAGG	1 cut(s)	1653
BsuI	GTATCC	1 cut(s)	2824
BsuRI	GGCC	4 cut(s)	377, 420, 925, 2013
BtgI	CCRYGG	1 cut(s)	2094
BtsCI	GGATG	4 cut(s)	711, 2422, 2672, 2819
BtsIMutI	CAGTG	9 cut(s)	120, 301, 363, 675, 853, 867, 1893, 2021, 2172
Cac8I	GCNNGC	4 cut(s)	350, 740, 1662, 1948
CaiI	CAGNNNCTG	1 cut(s)	77
CfoI	GCGC	1 cut(s)	1832
Cfr13I	GGNCC	4 cut(s)	492, 563, 923, 1715
Csp6I	GTAC	5 cut(s)	1396, 1985, 2302, 2368, 2541
CspCI	CAANNNNNGTGG	6 cut(s)	633, 668, 843, 878, 1065, 1100
CviQI	GTAC	5 cut(s)	1396, 1985, 2302, 2368, 2541
DraI	TTTAAA	1 cut(s)	1710
EaeI	YGGCCR	2 cut(s)	375, 418
Eam1104I	CTCTTC	4 cut(s)	248, 836, 1044, 2066
EarI	CTCTTC	4 cut(s)	248, 836, 1044, 2066
Ecl136II	GAGCTC	1 cut(s)	118
Eco130I	CCWWGG	2 cut(s)	716, 2094
Eco24I	GRGCYC	1 cut(s)	120
Eco31I	GGTCTC	1 cut(s)	2032
Eco47I	GGWCC	3 cut(s)	492, 563, 1715
Eco53kI	GAGCTC	1 cut(s)	118
Eco81I	CCTNAGG	1 cut(s)	1653
Eco88I	CYCGRG	2 cut(s)	2519, 2774
EcoICRI	GAGCTC	1 cut(s)	118
EcoNI	CCTNNNNNAGG	1 cut(s)	1422
EcoO109I	RGGNCCY	1 cut(s)	492
EcoRI	GAATTC	1 cut(s)	2662
EcoRII	CCWGG	4 cut(s)	160, 372, 702, 1879
EcoT14I	CCWWGG	2 cut(s)	716, 2094
EcoT22I	ATGCAT	1 cut(s)	472
EcoT38I	GRGCYC	1 cut(s)	120
ErhI	CCWWGG	2 cut(s)	716, 2094
FaqI	GGGAC	2 cut(s)	478, 1801
FauI	CCCGC	1 cut(s)	2563
FauNDI	CATATG	2 cut(s)	678, 1324
FblI	GTMKAC	3 cut(s)	1121, 2564, 2610
Fnu4HI	GCNGC	6 cut(s)	63, 75, 1141, 2005, 2210, 2361
FokI	GGATG	4 cut(s)	698, 2409, 2659, 2806
FriOI	GRGCYC	1 cut(s)	120
Fsp4HI	GCNGC	6 cut(s)	63, 75, 1141, 2005, 2210, 2361
FspBI	CTAG	6 cut(s)	239, 944, 1601, 1688, 1979, 2796
GlaI	GCGC	1 cut(s)	1831
GluI	GCNGC	6 cut(s)	63, 75, 1141, 2005, 2210, 2361
GsaI	CCCAGC	2 cut(s)	431, 549
GsuI	CTGGAG	2 cut(s)	183, 453
HaeIII	GGCC	4 cut(s)	377, 420, 925, 2013
HapII	CCGG	6 cut(s)	276, 345, 403, 417, 441, 921
HhaI	GCGC	1 cut(s)	1832
Hin6I	GCGC	1 cut(s)	1830
HinP1I	GCGC	1 cut(s)	1830
HincII	GTYRAC	2 cut(s)	289, 2658
HindII	GTYRAC	2 cut(s)	289, 2658
HindIII	AAGCTT	9 cut(s)	388, 812, 875, 892, 1388, 1747, 1919, 2174, 2840
HpaII	CCGG	6 cut(s)	276, 345, 403, 417, 441, 921
HphI	GGTGA	6 cut(s)	295, 361, 799, 1295, 2392, 2402
Hpy166II	GTNNAC	7 cut(s)	289, 369, 865, 1122, 2565, 2611, 2658
Hpy8I	GTNNAC	7 cut(s)	289, 369, 865, 1122, 2565, 2611, 2658
HpyAV	CCTTC	7 cut(s)	28, 107, 608, 1001, 1734, 2417, 2821
HpyCH4III	ACNGT	6 cut(s)	670, 966, 1562, 1941, 2161, 2582
HpyF10VI	GCNNNNNNNGC	3 cut(s)	71, 735, 2010
HspAI	GCGC	1 cut(s)	1830
KflI	GGGWCCC	1 cut(s)	492
KpnI	GGTACC	1 cut(s)	2544
LguI	GCTCTTC	1 cut(s)	2066
LmnI	GCTCC	6 cut(s)	357, 549, 1387, 1657, 2669, 2857
Lsp1109I	GCAGC	6 cut(s)	74, 86, 1152, 2016, 2196, 2372
LweI	GCATC	6 cut(s)	457, 720, 1176, 2293, 2521, 2681
MaeI	CTAG	6 cut(s)	239, 944, 1601, 1688, 1979, 2796
MaeIII	GTNAC	6 cut(s)	229, 329, 670, 1444, 1695, 2231
MfeI	CAATTG	2 cut(s)	1234, 2430
MflI	RGATCY	4 cut(s)	898, 1287, 2185, 2799
MhlI	GDGCHC	4 cut(s)	120, 1433, 2727, 2775
MlsI	TGGCCA	1 cut(s)	377
MluNI	TGGCCA	1 cut(s)	377
MlyI	GAGTC	7 cut(s)	294, 444, 788, 827, 979, 1043, 2499
MmeI	TCCRAC	1 cut(s)	2763
Mox20I	TGGCCA	1 cut(s)	377
Mph1103I	ATGCAT	1 cut(s)	472
MroXI	GAANNNNTTC	2 cut(s)	410, 2700
MscI	TGGCCA	1 cut(s)	377
MseI	TTAA	9 cut(s)	107, 140, 447, 1179, 1401, 1709, 2079, 2642, 2837
MslI	CAYNNNNRTG	3 cut(s)	1311, 1955, 2747
Msp20I	TGGCCA	1 cut(s)	377
MspA1I	CMGCKG	3 cut(s)	65, 77, 431
MspI	CCGG	6 cut(s)	276, 345, 403, 417, 441, 921
MspR9I	CCNGG	5 cut(s)	162, 374, 704, 922, 1881
MunI	CAATTG	2 cut(s)	1234, 2430
Mva1269I	GAATGC	2 cut(s)	470, 1143
MvaI	CCWGG	4 cut(s)	162, 374, 704, 1881
MwoI	GCNNNNNNNGC	3 cut(s)	71, 735, 2010
NciI	CCSGG	1 cut(s)	922
NcoI	CCATGG	1 cut(s)	2094
NdeI	CATATG	2 cut(s)	678, 1324
NmuCI	GTSAC	2 cut(s)	670, 1695
NsiI	ATGCAT	1 cut(s)	472
NspV	TTCGAA	1 cut(s)	1196
OliI	CACNNNNGTG	1 cut(s)	2747
PaeR7I	CTCGAG	2 cut(s)	2519, 2774
PciSI	GCTCTTC	1 cut(s)	2066
PctI	GAATGC	2 cut(s)	470, 1143
PdmI	GAANNNNTTC	2 cut(s)	410, 2700
PflFI	GACNNNGTC	1 cut(s)	284
PfoI	TCCNGGA	1 cut(s)	160
PkrI	GCNGC	6 cut(s)	64, 76, 1142, 2006, 2211, 2362
PleI	GAGTC	7 cut(s)	293, 443, 787, 826, 978, 1042, 2499
PpsI	GAGTC	7 cut(s)	293, 443, 787, 826, 978, 1042, 2499
PpuMI	RGGWCCY	1 cut(s)	492
Psp124BI	GAGCTC	1 cut(s)	120
Psp5II	RGGWCCY	1 cut(s)	492
Psp6I	CCWGG	4 cut(s)	160, 372, 702, 1879
PspFI	CCCAGC	2 cut(s)	427, 545
PspGI	CCWGG	4 cut(s)	160, 372, 702, 1879
PspPI	GGNCC	4 cut(s)	492, 563, 923, 1715
PspPPI	RGGWCCY	1 cut(s)	492
PspXI	VCTCGAGB	2 cut(s)	2519, 2774
PstI	CTGCAG	1 cut(s)	1444
PstNI	CAGNNNCTG	1 cut(s)	77
PsuI	RGATCY	4 cut(s)	898, 1287, 2185, 2799
PsyI	GACNNNGTC	1 cut(s)	284
PvuII	CAGCTG	3 cut(s)	65, 77, 431
RsaI	GTAC	5 cut(s)	1397, 1986, 2303, 2369, 2542
RsaNI	GTAC	5 cut(s)	1396, 1985, 2302, 2368, 2541
RseI	CAYNNNNRTG	3 cut(s)	1311, 1955, 2747
SacI	GAGCTC	1 cut(s)	120
SapI	GCTCTTC	1 cut(s)	2066
SaqAI	TTAA	9 cut(s)	107, 140, 447, 1179, 1401, 1709, 2079, 2642, 2837
SatI	GCNGC	6 cut(s)	63, 75, 1141, 2005, 2210, 2361
Sau96I	GGNCC	4 cut(s)	492, 563, 923, 1715
ScaI	AGTACT	2 cut(s)	1397, 1986
SchI	GAGTC	7 cut(s)	294, 444, 788, 827, 979, 1043, 2499
ScrFI	CCNGG	5 cut(s)	162, 374, 704, 922, 1881
SduI	GDGCHC	4 cut(s)	120, 1433, 2727, 2775
SfaNI	GCATC	6 cut(s)	457, 720, 1176, 2293, 2521, 2681
SfcI	CTRYAG	1 cut(s)	1440
Sfr274I	CTCGAG	2 cut(s)	2519, 2774
SfuI	TTCGAA	1 cut(s)	1196
SinI	GGWCC	3 cut(s)	492, 563, 1715
SlaI	CTCGAG	2 cut(s)	2519, 2774
SmiMI	CAYNNNNRTG	3 cut(s)	1311, 1955, 2747
SmlI	CTYRAG	9 cut(s)	527, 815, 1031, 1391, 1607, 2446, 2507, 2519, 2774
SmoI	CTYRAG	9 cut(s)	527, 815, 1031, 1391, 1607, 2446, 2507, 2519, 2774
SsiI	CCGC	2 cut(s)	2544, 2556
SspI	AATATT	1 cut(s)	2338
SspMI	CTAG	6 cut(s)	239, 944, 1601, 1688, 1979, 2796
SstI	GAGCTC	1 cut(s)	120
StyD4I	CCNGG	5 cut(s)	160, 372, 702, 920, 1879
StyI	CCWWGG	2 cut(s)	716, 2094
TaaI	ACNGT	6 cut(s)	670, 966, 1562, 1941, 2161, 2582
TaqI	TCGA	5 cut(s)	32, 1196, 2520, 2634, 2775
TaqII	GACCGA	2 cut(s)	297, 2829
TatI	WGTACW	3 cut(s)	1395, 1984, 2367
Tru1I	TTAA	9 cut(s)	107, 140, 447, 1179, 1401, 1709, 2079, 2642, 2837
Tru9I	TTAA	9 cut(s)	107, 140, 447, 1179, 1401, 1709, 2079, 2642, 2837
TscAI	CASTG	9 cut(s)	127, 301, 363, 675, 853, 867, 1893, 2021, 2172
TseFI	GTSAC	2 cut(s)	670, 1695
TseI	GCWGC	6 cut(s)	62, 74, 1140, 2004, 2209, 2360
Tsp45I	GTSAC	2 cut(s)	670, 1695
TspDTI	ATGAA	9 cut(s)	40, 266, 599, 1311, 1571, 2206, 2430, 2487, 2840
TspRI	CASTG	9 cut(s)	127, 301, 363, 675, 853, 867, 1893, 2021, 2172
Tth111I	GACNNNGTC	1 cut(s)	284
VpaK11BI	GGWCC	3 cut(s)	492, 563, 1715
XagI	CCTNNNNNAGG	1 cut(s)	1422
XapI	RAATTY	5 cut(s)	1081, 1471, 1843, 2498, 2662
XbaI	TCTAGA	2 cut(s)	238, 943
XcmI	CCANNNNNNNNNTGG	3 cut(s)	428, 683, 2092
XhoI	CTCGAG	2 cut(s)	2519, 2774
XmiI	GTMKAC	3 cut(s)	1121, 2564, 2610
XmnI	GAANNNNTTC	2 cut(s)	410, 2700
XspI	CTAG	6 cut(s)	239, 944, 1601, 1688, 1979, 2796
ZrmI	AGTACT	2 cut(s)	1397, 1986
Zsp2I	ATGCAT	1 cut(s)	472

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.