MD02G1012000.v1.1

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0014543
Physical Location & Seq
Reverse (-)
761782 .. 765478
3697 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1012000.v1.1.491

Sequence Viewer

Length: 3024 bp
ATGCATTTCTCACTCCGACTCTCACAGAAAAGGAAACGGATTTCCATAATATCTGACACATTTTTCTTCCAATCGGGCATCCAAAATTGTCTGTTCTCTCAGTCATCCCAACCGCAAGTTCCAATGGAGATTTTCCACTCCTCCTTCTACTTAATCCTTCTCCTCCCTCTTCTCCTCCTCTTCGCGGCGGTTTCCGCCGCGGATATCTACTCAGACACCCTTCTCAGCTTAAAATCCGAGCTCACAGATGCCAATGGCAGCTTGAAAAATTGGATACTCCCTTCTACAGAAAACCCACCTGGCAAAATCCACGCATGTTCTTGGTCCGGCGTCAAATGCAACACCAACAACTCCGCCATTACTGGCTTAGACCTCTCAGTAAAGAGTCTCGCCGGTGAAATTTCCGGCGATCAATTCGCCGTCTTCGCCGACCTTGTATCACTCAATCTCAGTCACAACTCGTTCTCCGGTCACCTCCCCGCCGGAATCTTCAACCTCACCAACCTCACAACATTAGACATCAGCAGAAACAACTTCTCCGGCCACTTCCCTACCGGCGTCTCCGCCCTCCAAAACCTCATTGTACTCGACGCATTCAGCAACAGCTTTTCTGGGTCGCTCCCCGCCGAAATATCAGACCTTCAGAACCTCAAAATCCTCAATTTAGCAGGAAGCTACTTCCGGGGACCGATCCCGTCTGAATACGGGGCTTTCCGCCGGCTCGAGTTTATTCATCTCGCCGGGAATATGATCACCGGAAGCATTCCGCCGGAACTGGGGAAGCTCAAAACAGTGACCCACATGGAGATTGGCTACAATTTTTACCACGGAAGCATTCCGTGGCAATTGGGGAACATGACGGAGCTCCAGTATCTCGACATTGCCTATGCAAATCTCTCCGGCGCTGTCCCAAAATCCCTCGGCGATCTCGACAATCTCGAATCCCTCTTCCTTTTCAGAAACCAGCTTTCTGGGTCAATTCCGCCTGAGCTCAGCAACATCAGAGCTCTCCAAAGCTTGGATCTTTCCGACAATCTGATTTCTGGGTGGATCCCAGAAACCTTTTCGGAGCTGAAAAACCTTAGACTCCTCAGTCTTTTCTACAACGAGATGAGCGGCGAAGTTCCCGACGGAATTGCAAAGCTTCCGTCTTTGGAGACGCTGCTTTTGTGGAACAATTTCTTCTCCGGCAACCTTCCCCGGAGCTTGGGGAGGAACTCGAAGCTCGGATGGGTAGACGTCTCCACAAACAACTTCAACGGCAGCGTTCCGCCTGATATCTGTGCCAATGGTCAGCTCCTTAAGCTGATGCTGTTTTCGAACAATTTCTCCGGCGATCTCTCCACTTCTCTGTCAAACTGTTCGTCTCTGGTTCGTCTTCGACTCGAAGATAACAAGTTTTCAGGTGAGATTCCATTGAAATTCAGCACTCTGACTGACATCACATATGTTGATTTATCTGGGAACAATTTTAGTGGTGGGATTCCAATGGATATTTCAAAAGCTCAAAAGCTTGAGTACTTCAATGTCTCTAGAAATCCAGAATTAGGGGGCACAGTTCCTGCAGAAATGTGGGAATTGCCAAATCTTAACAATTTTTCGGCTTCCTCGTGTGGCTTGATTGGCAATGTTCCTTCGTTCAAAAACTGCAAATCAATATCTGTTGTTGAATTGAGCAAGAACAGCTTGGAAGGAACTGTCCCGAAAAGTATTTCTAGCTGCCGGGTTCTTGAGAGGATTGATTTAGGTAGCAATAATATATCCGGTCGTATACCCGAGGAGCTTGCAAGTCTTCCTGCTCTTCTTGTTCTTGACCTGTCACATAACAGCTTCAATGGACCAATACCTAAAACTTTTGGTTCTTCTTCGAGTTTAGTACTTCTAAATGTGTCTTACAATGATGTTTCCGGGAGTATTCCATCCGAAAAGGTATTTAGAATGATGGAACGAAGCGCGTTTGATGGGAATCCAAAGCTGTGTGGAGAGCCTCTCAGATCATGTCCTGGCTCAGTGTCTATATTTGGAAGCAGAGTCGCCGGGAAGCTTCTGTGGATCGCACTACTCTGCATCGGAGTAGTTATGTTCGTTACTCTATCAGTTATGGGGATATTTTACTACCATAAAGGCAGCAAAGGCCAGTGGAAGATGATCTCGTTTGCTGGACTTCCTCAATTCACAAAAAATGATTTATTGAAGAGCTTCAGTTCGACAGAATCCATGGATGCATTGCCACCTTCGTCAGCTTCAGTGCGGAAAGCAGTTCTGCCAATGGGAATCGCAGTTTCGGTAAAGAAGATTGAATGGGGAGCAAAGAGAATGAGAGTTATGCTGGAATTTGTAACACAAATAGGAAATGCAAGGCATAAGAATTTAATTCGATTGCTTGGAGTTTGCTACAACAACCATCTGGCTTATCTCTTATACGATTACTTGCCCAATGGAAACTTGGCTGAAATGATAAGTGTGAAAAGCGAATGGGCAGCCAAGTACAAAATTATCATCGGCATTGCAAAGGGATTACACTTCCTTCACCACGAATGTTCTCCTACAATAACACATGGAGATCTGAGATCAAGTAATATAGTGTTTGACGAAAATATGGAACCTCATTTGACAGAATTTGGGTTCAAGCACCTGCTCGAGTTGAACAAAAGTTCGTTTACGGCAGCAACTTCCACCTGGGATGCAGGTGATAACATCAGTGACACAAAAGAGGAGCTGTGCAGGGACGTATACAGCTTTGGGGAGATCATGCTGGAGATTCTAAGCAACGGCAGGTTGACAAATTTTGGCGCAAGCATACAGAGCAAGTCGAGGGAAGTTGTTCTGCGGGAAATCTACAACGAGAACGAAGCTGGTTCCGACATTTCAATGCAAGAGGAGATAAAACTGGCAATTGAGGTTGCTATGCTTTGCACCAGGAGTAGGCCATCCGATCGACCATCCATGGAAAATGCATTGAAGCTTTTATCCGAGTCGAAACTCCAGATGAAAAATAACCCAACTTTTGAAACTGCAGGGTGA

Protein Analysis

1008

Amino Acids

110.55

Weight (kDa)

6.14

Isoelectric Point (pI)

37.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 205 - 321 1.5e-08 Leucine-rich repeat region
LRR_14 PF23598 325 - 393 3.6e-06 Leucine-rich repeat region
LRR_8 PF13855 336 - 393 1.4e-08 Leucine rich repeat
LRR_8 PF13855 577 - 634 6.5e-07 Leucine rich repeat
Pkinase PF00069 757 - 988 5.4e-22 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 758 - 987 1.4e-23 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014543)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51350
fragaria_vesca FvH4_1g01520
malus_domestica MD02G1012000.v1.1 MD15G1157800.v1.1
prunus_persica Prupe.7G253000_v2.0.a1
pyrus_communis pycom02g01130 pycom15g14160
rosa_chinensis RchiOBHm_Chr2g0086441
rosa_laevigata RLG00000015759
rosa_multiflora Rmu_sc0005127.1_g000003
rosa_roxburghii Rroxscaffold_2G00154570
rosa_rugosa Rorug01G0467300
rosa_samantha Rh2AG018100 Rh2BG017600 Rh2CG018500 Rh2DG019400
rosa_wichuraiana Rw2G001500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 2643, 2678
AasI GACNNNNNNGTC 1 cut(s) 1092
AatII GACGTC 1 cut(s) 1242
Acc36I ACCTGC 3 cut(s) 2643, 2678, 2766
AccB7I CCANNNNNTGG 1 cut(s) 1018
AccBSI CCGCTC 1 cut(s) 1116
AccI GTMKAC 3 cut(s) 1236, 1771, 2733
AccII CGCG 3 cut(s) 185, 200, 1955
AclWI GGATC 5 cut(s) 685, 1029, 1045, 1058, 2060
AcoI YGGCCR 1 cut(s) 541
AcsI RAATTY 6 cut(s) 399, 1421, 2333, 2368, 2618, 2785
AcuI CTGAAG 3 cut(s) 626, 2185, 2229
AcyI GRCGYC 3 cut(s) 330, 558, 1239
AfaI GTAC 4 cut(s) 585, 1520, 1878, 2489
AfiI CCNNNNNNNGG 6 cut(s) 184, 776, 1018, 1207, 1547, 2925
AflII CTTAAG 1 cut(s) 1301
AjnI CCWGG 4 cut(s) 298, 2002, 2678, 2918
AjuI GAANNNNNNNTTGG 4 cut(s) 102, 134, 1480, 1512
AloI GAACNNNNNNTCC 2 cut(s) 1313, 1345
Alw21I GWGCWC 4 cut(s) 243, 867, 993, 1009
Alw26I GTCTC 6 cut(s) 392, 565, 1151, 1246, 1371, 1534
AlwI GGATC 5 cut(s) 685, 1029, 1045, 1058, 2060
AlwNI CAGNNNCTG 1 cut(s) 1562
Ama87I CYCGRG 3 cut(s) 722, 1775, 2639
AoxI GGCC 3 cut(s) 541, 2134, 2927
ApeKI GCWGC 7 cut(s) 258, 1162, 1263, 1719, 2127, 2480, 2666
ApoI RAATTY 6 cut(s) 399, 1421, 2333, 2368, 2618, 2785
ArsI GACNNNNNNTTYG 6 cut(s) 1133, 1150, 1165, 1182, 1349, 1381
Asp700I GAANNNNTTC 4 cut(s) 1178, 1325, 2198, 2823
AspLEI GCGC 3 cut(s) 905, 1955, 2795
AspS9I GGNCC 3 cut(s) 324, 686, 1838
AsuC2I CCSGG 6 cut(s) 683, 742, 1201, 1724, 1909, 2038
AsuHPI GGTGA 7 cut(s) 407, 464, 490, 745, 1418, 2522, 2702
AsuII TTCGAA 1 cut(s) 1319
AvaI CYCGRG 3 cut(s) 722, 1775, 2639
AvaII GGWCC 3 cut(s) 324, 686, 1838
BaeGI GKGCMC 1 cut(s) 1556
BamHI GGATCC 1 cut(s) 1050
BanII GRGCYC 4 cut(s) 243, 867, 993, 1009
BauI CACGAG 1 cut(s) 1609
BbsI GAAGAC 3 cut(s) 415, 1370, 1784
Bbv12I GWGCWC 4 cut(s) 243, 867, 993, 1009
BbvI GCAGC 7 cut(s) 270, 1149, 1275, 1706, 2139, 2492, 2678
BccI CCATC 7 cut(s) 1224, 1927, 1936, 1955, 2412, 2938, 2950
BceAI ACGGC 4 cut(s) 404, 1276, 2679, 2788
BcgI CGANNNNNNTGC 4 cut(s) 1118, 1152, 1766, 1800
BciT130I CCWGG 4 cut(s) 300, 2004, 2680, 2920
BciVI GTATCC 1 cut(s) 267
BclI TGATCA 1 cut(s) 750
BcnI CCSGG 6 cut(s) 683, 742, 1201, 1724, 1909, 2038
BcoDI GTCTC 6 cut(s) 392, 565, 1151, 1246, 1371, 1534
BfaI CTAG 2 cut(s) 1533, 1716
BfmI CTRYAG 3 cut(s) 285, 1563, 3015
BfoI RGCGCY 1 cut(s) 906
BfrI CTTAAG 1 cut(s) 1301
BfuAI ACCTGC 3 cut(s) 2643, 2678, 2766
BfuI GTATCC 1 cut(s) 267
BglII AGATCT 1 cut(s) 2563
BlpI GCTNAGC 1 cut(s) 992
BmcAI AGTACT 2 cut(s) 1520, 1878
Bme18I GGWCC 3 cut(s) 324, 686, 1838
BmeT110I CYCGRG 3 cut(s) 722, 1775, 2639
BmgT120I GGNCC 3 cut(s) 324, 686, 1838
BmiI GGNNCC 4 cut(s) 687, 1052, 2604, 2860
BmrI ACTGGG 1 cut(s) 785
BmsI GCATC 6 cut(s) 87, 238, 1299, 2076, 2212, 2674
BmuI ACTGGG 1 cut(s) 785
BpiI GAAGAC 3 cut(s) 415, 1370, 1784
BplI GAGNNNNNCTC 2 cut(s) 1974, 2006
BpmI CTGGAG 3 cut(s) 851, 2777, 2969
Bpu10I CCTNAGC 1 cut(s) 987
Bpu1102I GCTNAGC 1 cut(s) 992
Bpu14I TTCGAA 1 cut(s) 1319
BpuEI CTTGAG 2 cut(s) 1535, 1751
BpuMI CCSGG 6 cut(s) 683, 742, 1201, 1724, 1909, 2038
BsaBI GATNNNNATC 1 cut(s) 1965
BsaHI GRCGYC 3 cut(s) 330, 558, 1239
BsaWI WCCGGW 3 cut(s) 467, 755, 1763
BsaXI ACNNNNNCTCC 8 cut(s) 449, 479, 521, 551, 854, 884, 1313, 1343
Bsc4I CCNNNNNNNGG 6 cut(s) 184, 776, 1018, 1207, 1547, 2925
Bse118I RCCGGY 3 cut(s) 392, 554, 717
Bse1I ACTGG 5 cut(s) 367, 780, 868, 2137, 2895
Bse3DI GCAATG 4 cut(s) 879, 1633, 2225, 2505
Bse8I GATNNNNATC 1 cut(s) 1965
BseBI CCWGG 4 cut(s) 300, 2004, 2680, 2920
BseGI GGATG 8 cut(s) 78, 104, 1235, 1919, 2227, 2689, 2930, 2942
BseJI GATNNNNATC 1 cut(s) 1965
BseLI CCNNNNNNNGG 6 cut(s) 184, 776, 1018, 1207, 1547, 2925
BseMI GCAATG 4 cut(s) 879, 1633, 2225, 2505
BseNI ACTGG 5 cut(s) 367, 780, 868, 2137, 2895
BseRI GAGGAG 8 cut(s) 130, 152, 164, 167, 1079, 1793, 2729, 2894
BseSI GKGCMC 1 cut(s) 1556
BseXI GCAGC 7 cut(s) 270, 1149, 1275, 1706, 2139, 2492, 2678
BsgI GTGCAG 1 cut(s) 2743
Bsh1236I CGCG 3 cut(s) 185, 200, 1955
Bsh1285I CGRYCG 2 cut(s) 1768, 2938
BshFI GGCC 3 cut(s) 543, 2136, 2929
BsiEI CGRYCG 2 cut(s) 1768, 2938
BsiHKAI GWGCWC 4 cut(s) 243, 867, 993, 1009
BsiHKCI CYCGRG 3 cut(s) 722, 1775, 2639
BslFI GGGAC 4 cut(s) 699, 893, 1685, 2741
BslI CCNNNNNNNGG 6 cut(s) 184, 776, 1018, 1207, 1547, 2925
BsmAI GTCTC 6 cut(s) 392, 565, 1151, 1246, 1371, 1534
BsmBI CGTCTC 4 cut(s) 565, 1151, 1246, 1371
BsmFI GGGAC 4 cut(s) 699, 893, 1685, 2741
BsmI GAATGC 3 cut(s) 593, 762, 834
BsnI GGCC 3 cut(s) 543, 2136, 2929
BsoBI CYCGRG 3 cut(s) 722, 1775, 2639
Bsp119I TTCGAA 1 cut(s) 1319
Bsp1286I GDGCHC 5 cut(s) 243, 867, 993, 1009, 1556
Bsp1720I GCTNAGC 1 cut(s) 992
Bsp19I CCATGG 2 cut(s) 2217, 2946
BspANI GGCC 3 cut(s) 543, 2136, 2929
BspFNI CGCG 3 cut(s) 185, 200, 1955
BspLI GGNNCC 4 cut(s) 687, 1052, 2604, 2860
BspMAI CTGCAG 2 cut(s) 1567, 3019
BspMI ACCTGC 3 cut(s) 2643, 2678, 2766
BspPI GGATC 5 cut(s) 685, 1029, 1045, 1058, 2060
BspQI GCTCTTC 2 cut(s) 1806, 2189
BspT104I TTCGAA 1 cut(s) 1319
BspTI CTTAAG 1 cut(s) 1301
BsrBI CCGCTC 1 cut(s) 1116
BsrDI GCAATG 4 cut(s) 879, 1633, 2225, 2505
BsrFI RCCGGY 3 cut(s) 392, 554, 717
BsrI ACTGG 5 cut(s) 367, 780, 868, 2137, 2895
BssAI RCCGGY 3 cut(s) 392, 554, 717
BssNAI GTATAC 2 cut(s) 1772, 2734
BssNI GRCGYC 3 cut(s) 330, 558, 1239
BssSI CACGAG 1 cut(s) 1609
BssT1I CCWWGG 2 cut(s) 2217, 2946
Bst1107I GTATAC 2 cut(s) 1772, 2734
Bst2BI CACGAG 1 cut(s) 1609
Bst2UI CCWGG 4 cut(s) 300, 2004, 2680, 2920
Bst4CI ACNGT 4 cut(s) 793, 1361, 1558, 1699
Bst6I CTCTTC 5 cut(s) 174, 185, 953, 1806, 2189
BstACI GRCGYC 3 cut(s) 330, 558, 1239
BstAFI CTTAAG 1 cut(s) 1301
BstBI TTCGAA 1 cut(s) 1319
BstC8I GCNNGC 3 cut(s) 719, 1785, 2797
BstDSI CCRYGG 5 cut(s) 198, 826, 839, 2217, 2946
BstEII GGTNACC 1 cut(s) 470
BstF5I GGATG 8 cut(s) 78, 104, 1235, 1919, 2227, 2689, 2930, 2942
BstFNI CGCG 3 cut(s) 185, 200, 1955
BstH2I RGCGCY 1 cut(s) 906
BstHHI GCGC 3 cut(s) 905, 1955, 2795
BstMAI GTCTC 6 cut(s) 392, 565, 1151, 1246, 1371, 1534
BstMCI CGRYCG 2 cut(s) 1768, 2938
BstMWI GCNNNNNNNGC 8 cut(s) 194, 336, 425, 1303, 1623, 1683, 2133, 2805
BstNI CCWGG 4 cut(s) 300, 2004, 2680, 2920
BstNSI RCATGY 1 cut(s) 318
BstPI GGTNACC 1 cut(s) 470
BstSFI CTRYAG 3 cut(s) 285, 1563, 3015
BstSLI GKGCMC 1 cut(s) 1556
BstUI CGCG 3 cut(s) 185, 200, 1955
BstV1I GCAGC 7 cut(s) 270, 1149, 1275, 1706, 2139, 2492, 2678
BstV2I GAAGAC 3 cut(s) 415, 1370, 1784
BstX2I RGATCY 3 cut(s) 1021, 1050, 2563
BstXI CCANNNNNNTGG 1 cut(s) 971
BstYI RGATCY 3 cut(s) 1021, 1050, 2563
BstZ17I GTATAC 2 cut(s) 1772, 2734
BsuI GTATCC 1 cut(s) 267
BsuRI GGCC 3 cut(s) 543, 2136, 2929
BtgI CCRYGG 5 cut(s) 198, 826, 839, 2217, 2946
BtsCI GGATG 8 cut(s) 78, 104, 1235, 1919, 2227, 2689, 2930, 2942
BtsIMutI CAGTG 5 cut(s) 798, 2016, 2144, 2253, 2707
BveI ACCTGC 3 cut(s) 2643, 2678, 2766
Cac8I GCNNGC 3 cut(s) 719, 1785, 2797
CaiI CAGNNNCTG 1 cut(s) 1562
CfoI GCGC 3 cut(s) 905, 1955, 2795
Cfr10I RCCGGY 3 cut(s) 392, 554, 717
Cfr13I GGNCC 3 cut(s) 324, 686, 1838
Cfr42I CCGCGG 1 cut(s) 201
CseI GACGC 4 cut(s) 319, 547, 599, 1168
Csp6I GTAC 4 cut(s) 584, 1519, 1877, 2488
CspCI CAANNNNNGTGG 2 cut(s) 1456, 1491
CviAII CATG 8 cut(s) 315, 802, 856, 1998, 2218, 2558, 2752, 2947
CviQI GTAC 4 cut(s) 584, 1519, 1877, 2488
DrdI GACNNNNNNGTC 1 cut(s) 1092
DseDI GACNNNNNNGTC 1 cut(s) 1092
EaeI YGGCCR 1 cut(s) 541
Eam1104I CTCTTC 5 cut(s) 174, 185, 953, 1806, 2189
EarI CTCTTC 5 cut(s) 174, 185, 953, 1806, 2189
EciI GGCGGA 7 cut(s) 184, 343, 553, 704, 756, 972, 1260
Ecl136II GAGCTC 4 cut(s) 241, 865, 991, 1007
Eco130I CCWWGG 2 cut(s) 2217, 2946
Eco24I GRGCYC 4 cut(s) 243, 867, 993, 1009
Eco32I GATATC 2 cut(s) 205, 1279
Eco47I GGWCC 3 cut(s) 324, 686, 1838
Eco53kI GAGCTC 4 cut(s) 241, 865, 991, 1007
Eco57I CTGAAG 3 cut(s) 626, 2185, 2229
Eco88I CYCGRG 3 cut(s) 722, 1775, 2639
Eco91I GGTNACC 1 cut(s) 470
EcoICRI GAGCTC 4 cut(s) 241, 865, 991, 1007
EcoO65I GGTNACC 1 cut(s) 470
EcoRII CCWGG 4 cut(s) 298, 2002, 2678, 2918
EcoRV GATATC 2 cut(s) 205, 1279
EcoT14I CCWWGG 2 cut(s) 2217, 2946
EcoT22I ATGCAT 3 cut(s) 6, 2227, 2959
EcoT38I GRGCYC 4 cut(s) 243, 867, 993, 1009
ErhI CCWWGG 2 cut(s) 2217, 2946
Esp3I CGTCTC 4 cut(s) 565, 1151, 1246, 1371
FaeI CATG 8 cut(s) 318, 805, 859, 2001, 2221, 2561, 2755, 2950
FalI AAGNNNNNCTT 2 cut(s) 1670, 1702
FaqI GGGAC 4 cut(s) 699, 893, 1685, 2741
FatI CATG 8 cut(s) 314, 801, 855, 1997, 2217, 2557, 2751, 2946
FauI CCCGC 3 cut(s) 487, 631, 2823
FauNDI CATATG 1 cut(s) 1447
FbaI TGATCA 1 cut(s) 750
FblI GTMKAC 3 cut(s) 1236, 1771, 2733
FokI GGATG 8 cut(s) 65, 91, 1242, 1906, 2234, 2696, 2917, 2929
FriOI GRGCYC 4 cut(s) 243, 867, 993, 1009
FspBI CTAG 2 cut(s) 1533, 1716
GlaI GCGC 3 cut(s) 904, 1954, 2794
GsuI CTGGAG 3 cut(s) 851, 2777, 2969
HaeII RGCGCY 1 cut(s) 906
HaeIII GGCC 3 cut(s) 543, 2136, 2929
HgaI GACGC 4 cut(s) 319, 547, 599, 1168
HhaI GCGC 3 cut(s) 905, 1955, 2795
Hin1I GRCGYC 3 cut(s) 330, 558, 1239
Hin1II CATG 8 cut(s) 318, 805, 859, 2001, 2221, 2561, 2755, 2950
Hin6I GCGC 3 cut(s) 903, 1953, 2793
HinP1I GCGC 3 cut(s) 903, 1953, 2793
HincII GTYRAC 1 cut(s) 2781
HindII GTYRAC 1 cut(s) 2781
HindIII AAGCTT 5 cut(s) 1015, 1142, 1511, 2042, 2963
HphI GGTGA 7 cut(s) 407, 464, 490, 745, 1418, 2522, 2702
Hpy166II GTNNAC 5 cut(s) 1237, 1772, 2661, 2734, 2781
Hpy8I GTNNAC 5 cut(s) 1237, 1772, 2661, 2734, 2781
Hpy99I CGWCG 2 cut(s) 593, 1133
HpyCH4III ACNGT 4 cut(s) 793, 1361, 1558, 1699
HpyCH4IV ACGT 2 cut(s) 1239, 2730
HpyF10VI GCNNNNNNNGC 8 cut(s) 194, 336, 425, 1303, 1623, 1683, 2133, 2805
HpySE526I ACGT 2 cut(s) 1239, 2730
Hsp92I GRCGYC 3 cut(s) 330, 558, 1239
Hsp92II CATG 8 cut(s) 318, 805, 859, 2001, 2221, 2561, 2755, 2950
HspAI GCGC 3 cut(s) 903, 1953, 2793
KroI GCCGGC 1 cut(s) 717
KroNI GCCGGC 1 cut(s) 719
Ksp22I TGATCA 1 cut(s) 750
KspI CCGCGG 1 cut(s) 201
LguI GCTCTTC 2 cut(s) 1806, 2189
LmnI GCTCC 9 cut(s) 624, 862, 870, 1069, 1203, 1302, 1780, 2306, 2716
Lsp1109I GCAGC 7 cut(s) 270, 1149, 1275, 1706, 2139, 2492, 2678
LweI GCATC 6 cut(s) 87, 238, 1299, 2076, 2212, 2674
MaeI CTAG 2 cut(s) 1533, 1716
MaeII ACGT 2 cut(s) 1239, 2730
MaeIII GTNAC 7 cut(s) 452, 470, 793, 1818, 2086, 2338, 2702
MbiI CCGCTC 1 cut(s) 1116
MfeI CAATTG 2 cut(s) 845, 2895
MflI RGATCY 3 cut(s) 1021, 1050, 2563
MhlI GDGCHC 5 cut(s) 243, 867, 993, 1009, 1556
MlyI GAGTC 6 cut(s) 12, 394, 1080, 1377, 2040, 2984
MmeI TCCRAC 3 cut(s) 40, 1053, 2886
Mph1103I ATGCAT 3 cut(s) 6, 2227, 2959
MroNI GCCGGC 1 cut(s) 717
MroXI GAANNNNTTC 4 cut(s) 1178, 1325, 2198, 2823
MseI TTAA 5 cut(s) 152, 230, 1302, 1590, 2372
MslI CAYNNNNRTG 1 cut(s) 2870
MspA1I CMGCKG 1 cut(s) 200
MspCI CTTAAG 1 cut(s) 1301
MunI CAATTG 2 cut(s) 845, 2895
Mva1269I GAATGC 3 cut(s) 593, 762, 834
MvaI CCWGG 4 cut(s) 300, 2004, 2680, 2920
MvnI CGCG 3 cut(s) 185, 200, 1955
MwoI GCNNNNNNNGC 8 cut(s) 194, 336, 425, 1303, 1623, 1683, 2133, 2805
NaeI GCCGGC 1 cut(s) 719
NciI CCSGG 6 cut(s) 683, 742, 1201, 1724, 1909, 2038
NcoI CCATGG 2 cut(s) 2217, 2946
NdeI CATATG 1 cut(s) 1447
NgoMIV GCCGGC 1 cut(s) 717
NlaIII CATG 8 cut(s) 318, 805, 859, 2001, 2221, 2561, 2755, 2950
NlaIV GGNNCC 4 cut(s) 687, 1052, 2604, 2860
NmeAIII GCCGAG 1 cut(s) 900
NmuCI GTSAC 5 cut(s) 452, 470, 793, 1818, 2702
NsiI ATGCAT 3 cut(s) 6, 2227, 2959
NspI RCATGY 1 cut(s) 318
NspV TTCGAA 1 cut(s) 1319
PaeR7I CTCGAG 2 cut(s) 722, 2639
PaqCI CACCTGC 2 cut(s) 2643, 2678
PciSI GCTCTTC 2 cut(s) 1806, 2189
PcsI WCGNNNNNNNCGW 3 cut(s) 927, 936, 1607
PctI GAATGC 3 cut(s) 593, 762, 834
PdiI GCCGGC 1 cut(s) 719
PdmI GAANNNNTTC 4 cut(s) 1178, 1325, 2198, 2823
PfeI GAWTC 8 cut(s) 486, 941, 1411, 1483, 1966, 2213, 2274, 2761
PflMI CCANNNNNTGG 1 cut(s) 1018
PfoI TCCNGGA 1 cut(s) 1907
Ple19I CGATCG 1 cut(s) 2938
PleI GAGTC 6 cut(s) 12, 393, 1080, 1377, 2039, 2983
PpsI GAGTC 6 cut(s) 12, 393, 1080, 1377, 2039, 2983
Psp124BI GAGCTC 4 cut(s) 243, 867, 993, 1009
Psp6I CCWGG 4 cut(s) 298, 2002, 2678, 2918
PspEI GGTNACC 1 cut(s) 470
PspGI CCWGG 4 cut(s) 298, 2002, 2678, 2918
PspN4I GGNNCC 4 cut(s) 687, 1052, 2604, 2860
PspPI GGNCC 3 cut(s) 324, 686, 1838
PspXI VCTCGAGB 2 cut(s) 722, 2639
PstI CTGCAG 2 cut(s) 1567, 3019
PstNI CAGNNNCTG 1 cut(s) 1562
PsuI RGATCY 3 cut(s) 1021, 1050, 2563
PvuI CGATCG 1 cut(s) 2938
RsaI GTAC 4 cut(s) 585, 1520, 1878, 2489
RsaNI GTAC 4 cut(s) 584, 1519, 1877, 2488
RseI CAYNNNNRTG 1 cut(s) 2870
SacI GAGCTC 4 cut(s) 243, 867, 993, 1009
SacII CCGCGG 1 cut(s) 201
SapI GCTCTTC 2 cut(s) 1806, 2189
SaqAI TTAA 5 cut(s) 152, 230, 1302, 1590, 2372
Sau96I GGNCC 3 cut(s) 324, 686, 1838
ScaI AGTACT 2 cut(s) 1520, 1878
SchI GAGTC 6 cut(s) 12, 394, 1080, 1377, 2040, 2984
SduI GDGCHC 5 cut(s) 243, 867, 993, 1009, 1556
SfaNI GCATC 6 cut(s) 87, 238, 1299, 2076, 2212, 2674
SfcI CTRYAG 3 cut(s) 285, 1563, 3015
Sfr274I CTCGAG 2 cut(s) 722, 2639
Sfr303I CCGCGG 1 cut(s) 201
SfuI TTCGAA 1 cut(s) 1319
SgrAI CRCCGGYG 1 cut(s) 392
SgrBI CCGCGG 1 cut(s) 201
SinI GGWCC 3 cut(s) 324, 686, 1838
SlaI CTCGAG 2 cut(s) 722, 2639
SmiMI CAYNNNNRTG 1 cut(s) 2870
SmlI CTYRAG 5 cut(s) 722, 1301, 1514, 1730, 2639
SmoI CTYRAG 5 cut(s) 722, 1301, 1514, 1730, 2639
SspMI CTAG 2 cut(s) 1533, 1716
SstI GAGCTC 4 cut(s) 243, 867, 993, 1009
StyI CCWWGG 2 cut(s) 2217, 2946
TaaI ACNGT 4 cut(s) 793, 1361, 1558, 1699
TaiI ACGT 2 cut(s) 1242, 2733
TaqII GACCGA 1 cut(s) 703
TatI WGTACW 4 cut(s) 583, 1518, 1876, 2487
TauI GCSGC 3 cut(s) 188, 200, 1119
TfiI GAWTC 8 cut(s) 486, 941, 1411, 1483, 1966, 2213, 2274, 2761
Tru1I TTAA 5 cut(s) 152, 230, 1302, 1590, 2372
Tru9I TTAA 5 cut(s) 152, 230, 1302, 1590, 2372
TscAI CASTG 5 cut(s) 798, 2016, 2144, 2253, 2707
TseFI GTSAC 5 cut(s) 452, 470, 793, 1818, 2702
TseI GCWGC 7 cut(s) 258, 1162, 1263, 1719, 2127, 2480, 2666
Tsp45I GTSAC 5 cut(s) 452, 470, 793, 1818, 2702
TspDTI ATGAA 2 cut(s) 722, 3005
TspGWI ACGGA 6 cut(s) 52, 828, 843, 875, 1137, 1146
TspRI CASTG 5 cut(s) 798, 2016, 2144, 2253, 2707
Van91I CCANNNNNTGG 1 cut(s) 1018
Vha464I CTTAAG 1 cut(s) 1301
VpaK11BI GGWCC 3 cut(s) 324, 686, 1838
XapI RAATTY 6 cut(s) 399, 1421, 2333, 2368, 2618, 2785
XbaI TCTAGA 1 cut(s) 1532
XceI RCATGY 1 cut(s) 318
XcmI CCANNNNNNNNNTGG 2 cut(s) 806, 2443
XhoI CTCGAG 2 cut(s) 722, 2639
XmiI GTMKAC 3 cut(s) 1236, 1771, 2733
XmnI GAANNNNTTC 4 cut(s) 1178, 1325, 2198, 2823
XspI CTAG 2 cut(s) 1533, 1716
ZraI GACGTC 1 cut(s) 1240
ZrmI AGTACT 2 cut(s) 1520, 1878
Zsp2I ATGCAT 3 cut(s) 6, 2227, 2959
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.