Rroxscaffold_2G00154570

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0014543
Physical Location & Seq
Forward (+)
91153499 .. 91156697
3199 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00154570.1

Sequence Viewer

Length: 2868 bp
ATGGAGATTTCCCACTCCTTCTTCTTCTTCTACAATCTTCTTCTCTTGTTCATGTTCTCTGCAGCTGTTTCAGCAGCTGATCTTTACTCAGAGACCCTTCTGAGCTTAAAATCTGAGCTCACAGATGACTATGATACCTTACAAGACTGGTCTGTGCCTCCTGGACAAAACCCATCTGCGAAAATCTATGCCTGTTCTTGGTCTGGTGTCAAATGCAACAGCAACAACTCCTCTGTTACCAGTCTAGACCTGTCAACGAAGAGTCTTGCCGGTTCAATCTCCGGAGTCAACTTCAGTGTCTTTACTGATCTTCTTGAGCTGAACCTGAGTTACAACTCATTTTCCGGCAAGCTCCCACTGGGAATGTTCACCCTGGCCAACTTAACAAGCTTGGACATTAGCCGGAACAACTTCTCCGGCCAGTTCCCAGCTGGAATCTCCGGTCTTAACAATCTGGTTGTGTTTGATGCATTCAGCAACAGCTTTTCTGGGTCACTGCCTGCTGAGGTGTCTCAACTTCAGAATCTCAAGGTCCTGAATCTAGCTGGGAGCTACTTCAAGGGTCCAATCCCACTAGAATATGGTAATTTTCAAAGCCTTGAGTTTATTCATCTTGCAGGGAATATGCTCAGTGGGAACATACCACCTGAATTGGGCAAGCTCAAAACAGTGACTCATATGGAGATTGGCTACAATTCTTACCAGGGAGGCATCCCTTGGCAATTGGGAAACATGAGCCAGCTTCAGTATCTTGATATTGCTTATGCAAATCTCTCCGAGTCTATTCCAAGAGAGCTTGGGAATCTCACCAAGCTTGAGTCTCTCTTTCTCTTCAGAAACCAGCTTAGTGGATCAATACCAGTGGAGTTCAGTAAACTTCAATCTCTCACAAGCTTGGATCTTTCGGATAATGAAATCTCCGGGCCGATCCCGGATAGTCTTCTAGAGTTGAAGAACCTGAAACTGTTGAGTCTTTTCTTCAATGAGATGAGTGGTGCTGTTCCTGAAGGGATTGCTGAGCTTCCTTCACTGGAGACTCTTCTTATATGGAACAACTACTTCTCTGGGAATCTTCCACAGGATTTGGGGAGGAACTCCAACCTCATGTGGGTTGATGTGTCTACAAACAACTTCAATGGCAGCATTCCAGCAGATATCTGTGTGAAAGGTGTGCTCTTTAAGCTGATGCTGTTTTCGAATAATTTTAGTGGAAGTCTTGCACCATCTCTGTCCAATTGTTCTTCACTTGTTCGTCTTCGGCTTGAAGATAACTCATTCTCAGGTGAGATCCCTTTGAAATTCAGCAACTTTCGTGATCTTTCATATGTAGACTTGTCCAAGAATAAACTTACTGGTGGGATTCCAATAGATATAGACCAAGCTCCCAAGCTTGAGTACTTTAATGTGTCCAATAATCCAGAATTAGGGGGCACAATTCCTGCACAAACATGGTCTTTGCCAGTTCTTCAGAATTTCTCAGCTTCCTTGTGTAGTATCGTAGACAATCTTCCGGCATTTGGAAGCTGTAAATCCATATCTGTTATTGAATTGAACATGAACAGTTTATCAGGGGGTGTTCCAGACAGTGTTTCTAGCTGCCGAGCTCTTGAGAGATTTGGTTTGGCCAACAATAACTTATCAGGTCATATACCTGAGGAGCTTGCAAGTCTTCCTGCTCTGCGTGTCCTAGATCTATCACATAATAGTTTGGATGGACCGATACCTTCAAAGTTTGGTTCTTCTTCAAGCTTATCACTTCTGAATGTGTCTTTCAATGATATTTCTGGGACAATTCCCTCTGGAAAGGTAGTGAAGACTATGGGAAGTAGCGCATTTGTTGGAAATCCAAAGCTATGTGGAGAGCCTTTGAGATCATGTCCCGGTTCAGTGGCTATCTTCGGAAGCAGAGGCACCGGGAAGCTTATATGGGTCTTGCTACTGTGTGCAGGCATAGTTATGTTCCTTACTCTGTCAGTTCTAGGAGTACTTTATTTTTATAAAGGCAGCAAAGGCCAATGGAAGATGGTCTCATTTGCTGGACTCCCTCAATTCACAGCAAGCGATGTATTGAAGAGCTTCAGTTCCATAGAATCCATGGATGATGCATTGTCCCCGACCTCAGCATCAGTATCAGTATGCAAAGCGGTTCTGCCAACAGGAATCACAGTTTCAGTGAAGAAGTTTGAATGCGATCCCAAGATAGTGAAAGTTATGTTGCCATTCATAACCCGAATTGGGAGTGCAAGGCATAAGAATCTAATCCGATTGCTCGGATTTTGCTACAACAAGCATCTGGTTTATCTCTTGTACGATTACTTGCCCAACGGAAGTTTAGCAGAAAATATAAGAGTGAAAAGGGAATGGGCAGCTAAGTACAAAATTGTGGTGAGCATTGCAAGGGGATTGTGCTTCCTTCACCATGAATGTCATCCTGCAATTGGTCATGGAGACTTGAGATCAAGTAATATAATGTTTGACGAAAACATGGAACCGCATCTGACAGAATTCGGATTCAAGCATCTGCTGGAGTTGAACAAACCTTCAGGTACTGCAGAGACTTCCACCATGGATACAGGTGCTGCAAACAGTTCTGTAGAAGAGGAGCTATACAGAGATGTATACAGCTTCGGGGAGATCATGCTCGAAATTTTATCCAACGGCAGGTTGACGAATTCAGGAGCAAGCTTGCATAGCAAGTCGAGGGAAAGTGTTCTCAGAGAAATAATCAATGAGAATGAAGTTGGTTCCAACACTTCAGTGCAACAGGAGATAAAAAGGGTGCTCGAGGTAGCTACGCTCTGCACTAGGAGCCGACCATCTGATCGACCATCCATGGAGGATACATTAAAGCTTTTACAGGCTCGATAA

Protein Analysis

955

Amino Acids

104.0

Weight (kDa)

5.55

Isoelectric Point (pI)

34.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 169 - 277 1.1e-08 Leucine-rich repeat region
LRR_14 PF23598 284 - 376 3.2e-07 Leucine-rich repeat region
PK_Tyr_Ser-Thr PF07714 719 - 949 1.5e-20 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 722 - 949 1.6e-18 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014543)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51350
fragaria_vesca FvH4_1g01520
malus_domestica MD02G1012000.v1.1 MD15G1157800.v1.1
prunus_persica Prupe.7G253000_v2.0.a1
pyrus_communis pycom02g01130 pycom15g14160
rosa_chinensis RchiOBHm_Chr2g0086441
rosa_laevigata RLG00000015759
rosa_multiflora Rmu_sc0005127.1_g000003
rosa_roxburghii Rroxscaffold_2G00154570
rosa_rugosa Rorug01G0467300
rosa_samantha Rh2AG018100 Rh2BG017600 Rh2CG018500 Rh2DG019400
rosa_wichuraiana Rw2G001500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1998
Acc36I ACCTGC 1 cut(s) 2652
AccB1I GGYRCC 1 cut(s) 1910
AccI GTMKAC 4 cut(s) 1121, 1329, 1500, 2619
AccIII TCCGGA 1 cut(s) 281
AciI CCGC 2 cut(s) 2144, 2492
AclWI GGATC 5 cut(s) 859, 906, 922, 1282, 2186
AcoI YGGCCR 3 cut(s) 375, 418, 1623
AcsI RAATTY 5 cut(s) 1298, 1471, 2504, 2646, 2671
AcuI CTGAAG 9 cut(s) 277, 503, 728, 817, 1026, 1451, 2062, 2526, 2739
AfaI GTAC 5 cut(s) 1397, 1986, 2309, 2375, 2548
AfiI CCNNNNNNNGG 8 cut(s) 198, 653, 1108, 1424, 1517, 2235, 2438, 2661
AjnI CCWGG 3 cut(s) 160, 372, 702
AleI CACNNNNGTG 1 cut(s) 2756
AloI GAACNNNNNNTCC 2 cut(s) 398, 430
Alw21I GWGCWC 4 cut(s) 120, 1176, 1606, 2784
Alw26I GTCTC 7 cut(s) 86, 516, 825, 1028, 2032, 2442, 2549
AlwI GGATC 5 cut(s) 859, 906, 922, 1282, 2186
AlwNI CAGNNNCTG 3 cut(s) 77, 2549, 2579
Ama87I CYCGRG 1 cut(s) 2783
Aor13HI TCCGGA 1 cut(s) 281
AoxI GGCC 5 cut(s) 375, 418, 923, 1623, 2011
ApeKI GCWGC 7 cut(s) 62, 74, 1140, 1596, 2004, 2366, 2579
ApoI RAATTY 5 cut(s) 1298, 1471, 2504, 2646, 2671
Asp700I GAANNNNTTC 3 cut(s) 410, 2075, 2709
AspLEI GCGC 1 cut(s) 1832
AspS9I GGNCC 4 cut(s) 532, 563, 923, 1715
AsuC2I CCSGG 4 cut(s) 922, 932, 1881, 1915
AsuHPI GGTGA 5 cut(s) 361, 799, 1295, 2398, 2408
AsuII TTCGAA 1 cut(s) 1196
AvaI CYCGRG 1 cut(s) 2783
AvaII GGWCC 3 cut(s) 532, 563, 1715
AxyI CCTNAGG 1 cut(s) 1653
BaeGI GKGCMC 1 cut(s) 1433
BalI TGGCCA 2 cut(s) 377, 1625
BanI GGYRCC 1 cut(s) 1910
BanII GRGCYC 2 cut(s) 120, 1606
BarI GAAGNNNNNNTAC 2 cut(s) 1491, 1523
BbsI GAAGAC 4 cut(s) 932, 1247, 1661, 1820
Bbv12I GWGCWC 4 cut(s) 120, 1176, 1606, 2784
BbvCI CCTCAGC 2 cut(s) 504, 2119
BbvI GCAGC 7 cut(s) 74, 86, 1152, 1583, 2016, 2378, 2566
BccI CCATC 6 cut(s) 181, 1231, 1706, 2017, 2824, 2836
BceAI ACGGC 1 cut(s) 2674
BciT130I CCWGG 3 cut(s) 162, 374, 704
BciVI GTATCC 2 cut(s) 2563, 2833
BcnI CCSGG 4 cut(s) 922, 932, 1881, 1915
BcoDI GTCTC 7 cut(s) 86, 516, 825, 1028, 2032, 2442, 2549
BfaI CTAG 8 cut(s) 245, 542, 575, 944, 1593, 1688, 1979, 2805
BfmI CTRYAG 3 cut(s) 60, 2550, 2592
BfuAI ACCTGC 1 cut(s) 2652
BfuI GTATCC 2 cut(s) 2563, 2833
BglII AGATCT 1 cut(s) 1690
BisI GCNGC 7 cut(s) 63, 75, 1141, 1597, 2005, 2367, 2580
BlpI GCTNAGC 1 cut(s) 1017
BlsI GCNGC 7 cut(s) 64, 76, 1142, 1598, 2006, 2368, 2581
BmcAI AGTACT 2 cut(s) 1397, 1986
Bme1390I CCNGG 7 cut(s) 162, 374, 704, 922, 932, 1881, 1915
Bme18I GGWCC 3 cut(s) 532, 563, 1715
BmeT110I CYCGRG 1 cut(s) 2783
BmgT120I GGNCC 4 cut(s) 532, 563, 923, 1715
BmiI GGNNCC 5 cut(s) 564, 1912, 2490, 2746, 2810
BmrFI CCNGG 7 cut(s) 162, 374, 704, 922, 932, 1881, 1915
BmrI ACTGGG 1 cut(s) 368
BmsI GCATC 8 cut(s) 457, 720, 1176, 2092, 2132, 2299, 2503, 2527
BmuI ACTGGG 1 cut(s) 368
BpiI GAAGAC 4 cut(s) 932, 1247, 1661, 1820
BpmI CTGGAG 2 cut(s) 1052, 2546
Bpu10I CCTNAGC 2 cut(s) 504, 2119
Bpu1102I GCTNAGC 1 cut(s) 1017
Bpu14I TTCGAA 1 cut(s) 1196
BpuEI CTTGAG 7 cut(s) 335, 512, 620, 836, 1412, 1628, 2473
BpuMI CCSGG 4 cut(s) 922, 932, 1881, 1915
BsaI GGTCTC 2 cut(s) 86, 2032
BsaJI CCNNGG 6 cut(s) 372, 703, 716, 2094, 2565, 2832
BsaWI WCCGGW 2 cut(s) 281, 440
BsaXI ACNNNNNCTCC 2 cut(s) 398, 428
Bsc4I CCNNNNNNNGG 8 cut(s) 198, 653, 1108, 1424, 1517, 2235, 2438, 2661
Bse118I RCCGGY 1 cut(s) 269
Bse1I ACTGG 8 cut(s) 152, 240, 363, 421, 860, 1035, 1357, 1460
Bse21I CCTNAGG 1 cut(s) 1653
Bse3DI GCAATG 1 cut(s) 2391
BseAI TCCGGA 1 cut(s) 281
BseBI CCWGG 3 cut(s) 162, 374, 704
BseDI CCNNGG 6 cut(s) 372, 703, 716, 2094, 2565, 2832
BseGI GGATG 5 cut(s) 711, 1717, 2104, 2428, 2828
BseLI CCNNNNNNNGG 8 cut(s) 198, 653, 1108, 1424, 1517, 2235, 2438, 2661
BseMI GCAATG 1 cut(s) 2391
BseNI ACTGG 8 cut(s) 152, 240, 363, 421, 860, 1035, 1357, 1460
BseRI GAGGAG 3 cut(s) 220, 1670, 2615
BseSI GKGCMC 1 cut(s) 1433
BseXI GCAGC 7 cut(s) 74, 86, 1152, 1583, 2016, 2378, 2566
BseYI CCCAGC 2 cut(s) 427, 545
BsgI GTGCAG 3 cut(s) 1425, 1965, 2785
BshFI GGCC 5 cut(s) 377, 420, 925, 1625, 2013
BshNI GGYRCC 1 cut(s) 1910
BsiHKAI GWGCWC 4 cut(s) 120, 1176, 1606, 2784
BsiHKCI CYCGRG 1 cut(s) 2783
BslFI GGGAC 3 cut(s) 1801, 1863, 2095
BslI CCNNNNNNNGG 8 cut(s) 198, 653, 1108, 1424, 1517, 2235, 2438, 2661
BsmAI GTCTC 7 cut(s) 86, 516, 825, 1028, 2032, 2442, 2549
BsmFI GGGAC 3 cut(s) 1801, 1863, 2095
BsmI GAATGC 3 cut(s) 470, 1143, 2192
BsnI GGCC 5 cut(s) 377, 420, 925, 1625, 2013
Bso31I GGTCTC 2 cut(s) 86, 2032
BsoBI CYCGRG 1 cut(s) 2783
Bsp119I TTCGAA 1 cut(s) 1196
Bsp1286I GDGCHC 5 cut(s) 120, 1176, 1433, 1606, 2784
Bsp13I TCCGGA 1 cut(s) 281
Bsp1720I GCTNAGC 1 cut(s) 1017
Bsp19I CCATGG 3 cut(s) 2094, 2565, 2832
BspACI CCGC 2 cut(s) 2144, 2492
BspANI GGCC 5 cut(s) 377, 420, 925, 1625, 2013
BspEI TCCGGA 1 cut(s) 281
BspLI GGNNCC 5 cut(s) 564, 1912, 2490, 2746, 2810
BspMAI CTGCAG 2 cut(s) 64, 2554
BspMI ACCTGC 1 cut(s) 2652
BspPI GGATC 5 cut(s) 859, 906, 922, 1282, 2186
BspQI GCTCTTC 1 cut(s) 2066
BspT104I TTCGAA 1 cut(s) 1196
BspT107I GGYRCC 1 cut(s) 1910
BspTNI GGTCTC 2 cut(s) 86, 2032
BsrDI GCAATG 1 cut(s) 2391
BsrFI RCCGGY 1 cut(s) 269
BsrI ACTGG 8 cut(s) 152, 240, 363, 421, 860, 1035, 1357, 1460
BssAI RCCGGY 1 cut(s) 269
BssECI CCNNGG 6 cut(s) 372, 703, 716, 2094, 2565, 2832
BssNAI GTATAC 1 cut(s) 2620
BssT1I CCWWGG 4 cut(s) 716, 2094, 2565, 2832
Bst1107I GTATAC 1 cut(s) 2620
Bst2UI CCWGG 3 cut(s) 162, 374, 704
Bst4CI ACNGT 7 cut(s) 670, 966, 1562, 1586, 1941, 2167, 2588
Bst6I CTCTTC 5 cut(s) 254, 836, 1044, 2066, 2592
BstBI TTCGAA 1 cut(s) 1196
BstC8I GCNNGC 9 cut(s) 350, 501, 659, 740, 1662, 1948, 2059, 2683, 2687
BstDSI CCRYGG 3 cut(s) 2094, 2565, 2832
BstF5I GGATG 5 cut(s) 711, 1717, 2104, 2428, 2828
BstHHI GCGC 1 cut(s) 1832
BstMAI GTCTC 7 cut(s) 86, 516, 825, 1028, 2032, 2442, 2549
BstMWI GCNNNNNNNGC 5 cut(s) 71, 1180, 2010, 2691, 2808
BstNI CCWGG 3 cut(s) 162, 374, 704
BstSCI CCNGG 7 cut(s) 160, 372, 702, 920, 930, 1879, 1913
BstSFI CTRYAG 3 cut(s) 60, 2550, 2592
BstSLI GKGCMC 1 cut(s) 1433
BstV1I GCAGC 7 cut(s) 74, 86, 1152, 1583, 2016, 2378, 2566
BstV2I GAAGAC 4 cut(s) 932, 1247, 1661, 1820
BstX2I RGATCY 3 cut(s) 898, 1287, 1690
BstXI CCANNNNNNTGG 1 cut(s) 848
BstYI RGATCY 3 cut(s) 898, 1287, 1690
BstZ17I GTATAC 1 cut(s) 2620
Bsu36I CCTNAGG 1 cut(s) 1653
BsuI GTATCC 2 cut(s) 2563, 2833
BsuRI GGCC 5 cut(s) 377, 420, 925, 1625, 2013
BtgI CCRYGG 3 cut(s) 2094, 2565, 2832
BtgZI GCGATG 1 cut(s) 2076
BtsCI GGATG 5 cut(s) 711, 1717, 2104, 2428, 2828
BtsI GCAGTG 1 cut(s) 494
BveI ACCTGC 1 cut(s) 2652
Cac8I GCNNGC 9 cut(s) 350, 501, 659, 740, 1662, 1948, 2059, 2683, 2687
CaiI CAGNNNCTG 3 cut(s) 77, 2549, 2579
CfoI GCGC 1 cut(s) 1832
Cfr10I RCCGGY 1 cut(s) 269
Cfr13I GGNCC 4 cut(s) 532, 563, 923, 1715
Csp6I GTAC 5 cut(s) 1396, 1985, 2308, 2374, 2547
CspCI CAANNNNNGTGG 6 cut(s) 633, 668, 843, 878, 1065, 1100
CviQI GTAC 5 cut(s) 1396, 1985, 2308, 2374, 2547
EaeI YGGCCR 3 cut(s) 375, 418, 1623
Eam1104I CTCTTC 5 cut(s) 254, 836, 1044, 2066, 2592
EarI CTCTTC 5 cut(s) 254, 836, 1044, 2066, 2592
Ecl136II GAGCTC 2 cut(s) 118, 1604
Eco130I CCWWGG 4 cut(s) 716, 2094, 2565, 2832
Eco24I GRGCYC 2 cut(s) 120, 1606
Eco31I GGTCTC 2 cut(s) 86, 2032
Eco32I GATATC 1 cut(s) 1156
Eco47I GGWCC 3 cut(s) 532, 563, 1715
Eco53kI GAGCTC 2 cut(s) 118, 1604
Eco57I CTGAAG 9 cut(s) 277, 503, 728, 817, 1026, 1451, 2062, 2526, 2739
Eco81I CCTNAGG 1 cut(s) 1653
Eco88I CYCGRG 1 cut(s) 2783
EcoICRI GAGCTC 2 cut(s) 118, 1604
EcoO109I RGGNCCY 1 cut(s) 532
EcoRI GAATTC 2 cut(s) 2504, 2671
EcoRII CCWGG 3 cut(s) 160, 372, 702
EcoRV GATATC 1 cut(s) 1156
EcoT14I CCWWGG 4 cut(s) 716, 2094, 2565, 2832
EcoT22I ATGCAT 2 cut(s) 472, 2107
EcoT38I GRGCYC 2 cut(s) 120, 1606
ErhI CCWWGG 4 cut(s) 716, 2094, 2565, 2832
FaqI GGGAC 3 cut(s) 1801, 1863, 2095
FauNDI CATATG 2 cut(s) 678, 1324
FblI GTMKAC 4 cut(s) 1121, 1329, 1500, 2619
Fnu4HI GCNGC 7 cut(s) 63, 75, 1141, 1597, 2005, 2367, 2580
FokI GGATG 5 cut(s) 698, 1724, 2111, 2415, 2815
FriOI GRGCYC 2 cut(s) 120, 1606
Fsp4HI GCNGC 7 cut(s) 63, 75, 1141, 1597, 2005, 2367, 2580
FspBI CTAG 8 cut(s) 245, 542, 575, 944, 1593, 1688, 1979, 2805
GlaI GCGC 1 cut(s) 1831
GluI GCNGC 7 cut(s) 63, 75, 1141, 1597, 2005, 2367, 2580
GsaI CCCAGC 2 cut(s) 431, 549
GsuI CTGGAG 2 cut(s) 1052, 2546
HaeIII GGCC 5 cut(s) 377, 420, 925, 1625, 2013
HhaI GCGC 1 cut(s) 1832
Hin6I GCGC 1 cut(s) 1830
HinP1I GCGC 1 cut(s) 1830
HincII GTYRAC 3 cut(s) 255, 289, 2667
HindII GTYRAC 3 cut(s) 255, 289, 2667
HindIII AAGCTT 8 cut(s) 388, 812, 892, 1388, 1747, 1919, 2683, 2849
HphI GGTGA 5 cut(s) 361, 799, 1295, 2398, 2408
Hpy166II GTNNAC 9 cut(s) 255, 289, 369, 875, 1122, 1330, 1501, 2620, 2667
Hpy8I GTNNAC 9 cut(s) 255, 289, 369, 875, 1122, 1330, 1501, 2620, 2667
HpyAV CCTTC 7 cut(s) 28, 107, 1001, 1035, 1734, 2423, 2550
HpyCH4III ACNGT 7 cut(s) 670, 966, 1562, 1586, 1941, 2167, 2588
HpyF10VI GCNNNNNNNGC 5 cut(s) 71, 1180, 2010, 2691, 2808
HspAI GCGC 1 cut(s) 1830
Kpn2I TCCGGA 1 cut(s) 281
LguI GCTCTTC 1 cut(s) 2066
LmnI GCTCC 7 cut(s) 357, 549, 1387, 1657, 2602, 2678, 2808
Lsp1109I GCAGC 7 cut(s) 74, 86, 1152, 1583, 2016, 2378, 2566
LweI GCATC 8 cut(s) 457, 720, 1176, 2092, 2132, 2299, 2503, 2527
MaeI CTAG 8 cut(s) 245, 542, 575, 944, 1593, 1688, 1979, 2805
MaeIII GTNAC 4 cut(s) 235, 329, 492, 670
MfeI CAATTG 3 cut(s) 722, 1234, 2436
MflI RGATCY 3 cut(s) 898, 1287, 1690
MhlI GDGCHC 5 cut(s) 120, 1176, 1433, 1606, 2784
MlsI TGGCCA 2 cut(s) 377, 1625
MluNI TGGCCA 2 cut(s) 377, 1625
MlyI GAGTC 8 cut(s) 271, 294, 667, 788, 827, 979, 1030, 2034
MmeI TCCRAC 4 cut(s) 1122, 1819, 2679, 2772
Mox20I TGGCCA 2 cut(s) 377, 1625
Mph1103I ATGCAT 2 cut(s) 472, 2107
MroI TCCGGA 1 cut(s) 281
MroXI GAANNNNTTC 3 cut(s) 410, 2075, 2709
MscI TGGCCA 2 cut(s) 377, 1625
MseI TTAA 6 cut(s) 107, 383, 447, 1179, 1401, 2846
MslI CAYNNNNRTG 3 cut(s) 1447, 1955, 2756
Msp20I TGGCCA 2 cut(s) 377, 1625
MspA1I CMGCKG 3 cut(s) 65, 77, 431
MspR9I CCNGG 7 cut(s) 162, 374, 704, 922, 932, 1881, 1915
MunI CAATTG 3 cut(s) 722, 1234, 2436
Mva1269I GAATGC 3 cut(s) 470, 1143, 2192
MvaI CCWGG 3 cut(s) 162, 374, 704
MwoI GCNNNNNNNGC 5 cut(s) 71, 1180, 2010, 2691, 2808
NciI CCSGG 4 cut(s) 922, 932, 1881, 1915
NcoI CCATGG 3 cut(s) 2094, 2565, 2832
NdeI CATATG 2 cut(s) 678, 1324
NlaIV GGNNCC 5 cut(s) 564, 1912, 2490, 2746, 2810
NmeAIII GCCGAG 1 cut(s) 1625
NmuCI GTSAC 2 cut(s) 492, 670
NsiI ATGCAT 2 cut(s) 472, 2107
NspV TTCGAA 1 cut(s) 1196
OliI CACNNNNGTG 1 cut(s) 2756
PaeR7I CTCGAG 1 cut(s) 2783
PciSI GCTCTTC 1 cut(s) 2066
PctI GAATGC 3 cut(s) 470, 1143, 2192
PdmI GAANNNNTTC 3 cut(s) 410, 2075, 2709
PfoI TCCNGGA 2 cut(s) 160, 930
PkrI GCNGC 7 cut(s) 64, 76, 1142, 1598, 2006, 2368, 2581
PleI GAGTC 8 cut(s) 270, 293, 667, 787, 826, 978, 1030, 2034
PpsI GAGTC 8 cut(s) 270, 293, 667, 787, 826, 978, 1030, 2034
PpuMI RGGWCCY 1 cut(s) 532
PsiI TTATAA 1 cut(s) 1998
Psp124BI GAGCTC 2 cut(s) 120, 1606
Psp5II RGGWCCY 1 cut(s) 532
Psp6I CCWGG 3 cut(s) 160, 372, 702
PspFI CCCAGC 2 cut(s) 427, 545
PspGI CCWGG 3 cut(s) 160, 372, 702
PspN4I GGNNCC 5 cut(s) 564, 1912, 2490, 2746, 2810
PspPI GGNCC 4 cut(s) 532, 563, 923, 1715
PspPPI RGGWCCY 1 cut(s) 532
PspXI VCTCGAGB 1 cut(s) 2783
PsrI GAACNNNNNNTAC 2 cut(s) 314, 346
PstI CTGCAG 2 cut(s) 64, 2554
PstNI CAGNNNCTG 3 cut(s) 77, 2549, 2579
PsuI RGATCY 3 cut(s) 898, 1287, 1690
PvuII CAGCTG 3 cut(s) 65, 77, 431
RsaI GTAC 5 cut(s) 1397, 1986, 2309, 2375, 2548
RsaNI GTAC 5 cut(s) 1396, 1985, 2308, 2374, 2547
RseI CAYNNNNRTG 3 cut(s) 1447, 1955, 2756
SacI GAGCTC 2 cut(s) 120, 1606
SapI GCTCTTC 1 cut(s) 2066
SaqAI TTAA 6 cut(s) 107, 383, 447, 1179, 1401, 2846
SatI GCNGC 7 cut(s) 63, 75, 1141, 1597, 2005, 2367, 2580
Sau96I GGNCC 4 cut(s) 532, 563, 923, 1715
ScaI AGTACT 2 cut(s) 1397, 1986
SchI GAGTC 8 cut(s) 271, 294, 667, 788, 827, 979, 1030, 2034
ScrFI CCNGG 7 cut(s) 162, 374, 704, 922, 932, 1881, 1915
SduI GDGCHC 5 cut(s) 120, 1176, 1433, 1606, 2784
SfaNI GCATC 8 cut(s) 457, 720, 1176, 2092, 2132, 2299, 2503, 2527
SfcI CTRYAG 3 cut(s) 60, 2550, 2592
Sfr274I CTCGAG 1 cut(s) 2783
SfuI TTCGAA 1 cut(s) 1196
SinI GGWCC 3 cut(s) 532, 563, 1715
SlaI CTCGAG 1 cut(s) 2783
SmiMI CAYNNNNRTG 3 cut(s) 1447, 1955, 2756
SmlI CTYRAG 8 cut(s) 314, 527, 599, 815, 1391, 1607, 2452, 2783
SmoI CTYRAG 8 cut(s) 314, 527, 599, 815, 1391, 1607, 2452, 2783
SsiI CCGC 2 cut(s) 2144, 2492
SspMI CTAG 8 cut(s) 245, 542, 575, 944, 1593, 1688, 1979, 2805
SstI GAGCTC 2 cut(s) 120, 1606
StyD4I CCNGG 7 cut(s) 160, 372, 702, 920, 930, 1879, 1913
StyI CCWWGG 4 cut(s) 716, 2094, 2565, 2832
TaaI ACNGT 7 cut(s) 670, 966, 1562, 1586, 1941, 2167, 2588
TaqI TCGA 6 cut(s) 1196, 2643, 2699, 2784, 2824, 2863
TaqII GACCGA 1 cut(s) 1732
TatI WGTACW 3 cut(s) 1395, 1984, 2373
Tru1I TTAA 6 cut(s) 107, 383, 447, 1179, 1401, 2846
Tru9I TTAA 6 cut(s) 107, 383, 447, 1179, 1401, 2846
TseFI GTSAC 2 cut(s) 492, 670
TseI GCWGC 7 cut(s) 62, 74, 1140, 1596, 2004, 2366, 2579
Tsp45I GTSAC 2 cut(s) 492, 670
TspDTI ATGAA 8 cut(s) 40, 599, 927, 1311, 1571, 2212, 2436, 2751
TspGWI ACGGA 1 cut(s) 2340
VpaK11BI GGWCC 3 cut(s) 532, 563, 1715
XapI RAATTY 5 cut(s) 1298, 1471, 2504, 2646, 2671
XbaI TCTAGA 2 cut(s) 244, 943
XcmI CCANNNNNNNNNTGG 2 cut(s) 428, 2092
XhoI CTCGAG 1 cut(s) 2783
XmiI GTMKAC 4 cut(s) 1121, 1329, 1500, 2619
XmnI GAANNNNTTC 3 cut(s) 410, 2075, 2709
XspI CTAG 8 cut(s) 245, 542, 575, 944, 1593, 1688, 1979, 2805
ZrmI AGTACT 2 cut(s) 1397, 1986
Zsp2I ATGCAT 2 cut(s) 472, 2107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.