RchiOBHm_Chr2g0086441

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0014543
Physical Location & Seq
Reverse (-)
1597624 .. 1600902
3279 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46196

Sequence Viewer

Length: 2859 bp
ATGGAGATTTCCCACTCCTTCTTCTTCTACAATCTTCTTCTCTTGTTCATGTTCTCTGCAGCTGTTTCAGCAGCTGATCTGTACTCAGAGACCCTTCTGAGCTTAAAGTCTGAGCTCACAGATGACTATGACAGCTTACAAGACTGGTCTGTGCCTCCTGGACAAAACCCATCTGCGAAAATCTATGCCTGTTCTTGGTCTGGTGTCAAATGCAACAGCAACAACTCCTCTGTTACCAGTCTAGACCTGTCAGTGAAGAGGCTTGCCGGTTCAATCTCCGGAGTCAACTTCAGTGTCTTTACTGATCTTCTTGACCTGAACCTGAGTTACAACTCATTTTCCGGCAAGCTCCCACTGGGAATGTTCACCCTGGCCAACTTAACAAGCTTGGACATTAGCCGGAACAACTTCTCCGGCCAGTTCCCAGCTGGAATCTCCGGTCTTACCAATCTGGTTGTGTTTGATGCATTCAGCAACAGCTTTTCTGGGTCACTGCCTGCTGAGGTGTCTCAACTTCAGAATCTCAAGGTCCTGAATCTAGCTGGGAGCTACTTCAAGGGTCCAATCCCACTAGAATATGGTAATTTTCAAAGCCTTGAGTTTATTCATCTTGCAGGGAATATGCTCAGTGGGAACATACCACCTGAATTGGGCAAGCTCAAAACAGTGACTCATATGGAGATTGGCTACAATTCTTACCAGGGAGGCATCCCTTGGCAATTGGGAAACATGAGCCAGCTTCAGTATCTTGATATTGCTTATGCAAATCTCTCTGAGTCTATTCCAAGAGAGCTTGGGAATCTCACCAAGCTTGAGTCACTCTTTCTCTTCAGAAACCAGCTTAGTGGATCAATACCAGTGGAGTTCAGTAAACTTCAATCTCTCACAAGCTTGGATCTTTCGGATAATGAAATCTCCGGGCCGATCCCGGATAGTCTTCTAGAGTTGAAGAACCTGAAACTGTTGAGTCTTTTCTACAATGAGATGAGTGGTGCTGTTCCTGAAGGGATTGCTGAGCTTCCTTCACTGGAGACTCTTCTTATATGGAACAACTACTTCTCTGGGAATCTTCCACAGGATTTGGGGAGGAACTCCAACCTCATGTGGGTTGATGTGTCTACAAACAACTTCAATGGCAGCATTCCAGCAGATATCTGTGTGAAAGGTGTGCTCTTTAAGCTGATGCTGTTTTCGAATAATTTTAGTGGCAGTCTTGCACCATCTCTGTCCAATTGTTCTTCACTTGTTCGTCTTCGGCTTGAAGATAACTCATTCTCAGGTGAGATCCCTTTGAAATTCAGCAACTTTCGTGATCTTTCATATGTAGACTTGTCCGAGAATAAACTCACTGGTGGGATTCCAATAGATATAGACCAAGCTCCCAAGCTTGAGTACTTTAATGTGTCCAATAATCCAGAATTAGGGGGCACAATTCCTGCACAAACATGGTCTTTGCCAGTTCTTCAGAATTTCTCAGCTTCCTCGTGTAGTATCGTAGGCAATCTTCCGGCATTTGGAAGCTGTAAATCCATATCTGTTATTGAATTGAACATGAACAGTTTATCAGGGGGTGTTCCAGACAGTGTTTCTAGCTGCCGAGCTCTTGAGAGATTTGGTTTGGCCAACAATAACTTATCAGGTCATATACCTGAGGAGCTTGCAAGTCTTCCTGCTCTGCGTGTCCTAGACCTATCACATAATAGTTTGGATGGACCAATACCTTCAAAGTTTGGTTCTTCTTCAAGCTTATCACTTCTGAATGTGTCTTTCAATGATATTTCTGGGACAATTCCCTCTGGAAAGGTAGTGAAGACTATGGGAAGTAGCGCATTCGTTGGAAATCCAAAGCTATGTGGAGAGCCTTTGAGATCATGTCCCGGTTCAGTGGCTATCTTCGGAAGCAGAGGCACCGGGAAGCTTATATGGGTCTTGCTACTGTGTGCAGGCATACTTATGTTCCTTGCTCTGTCAGCTCTAGGAGTACTTTATTTTTATAAAGGCAGCAAAGGCCAATGGAAGATGGTCTCATTTGCTGGACTCCCTCAATTCAAAGCAAGCGATGTATTGAAGAGCTTCAGTTCCATAGAATCCATGGATGATGCATCGTCCCCGACGTCAGCATCAGTATGCAAAGCGGTTCTGCCAACAGGAATCACAGTTTCAGTGAAGAAGTTTGAATGCGATCCGAAGCTAGTGAATGTTATGTTGCCATTCATAACCCGAATTGGGAGTGCAAGGCATAAGAATCTAATCCGATTGCTCGGATTTTGCTACAACAAGCATCTGGTTTATCTCTTGTACGATTACTTGCCCAACGGAAGTTTATCAGAAAATATAAGAGTGAAAAGGGAATGGGCAGCTAAGTACAAAATTGTGGTGAGCATTGCAAGGGGATTGTGCTTCCTTCACCATGAATGTCATCCTGCAGTTGGTCATGGGGACTTGAGATCAAGTAATATAATGTTTGACGAAAACATGGAACCGCATCTGACAGAATTCGGATTCAAGCATCTGCTGGAGTTGAACAAAACTTCAGGTACTGCAGAGACCTCCACCATGGATACAGGTGCTGCAAACAGTTCTGTAGAAGAGGAGCTATACAGAGATGTATACAGCTTCGGGGAGATCATGCTTGAAATTTTATCCAACGGCAGGTTGACGAATTCAGGAGCAAGCTTACATGGCAAGTCGAGGGAAAGTGTTCTCAGAGAAATAATCAATGAGAATGAAGTTGGTTCTAACACTTCAGTGCAACAGGAGATAAAAAGGGTGCTCGAGGTAGCTACGCTCTGCACTAGGAGCCGTCCATCTGATCGACCATCCATGGAGGATACATTAAAGCTTTTACAGGCTCGATAA

Protein Analysis

952

Amino Acids

103.55

Weight (kDa)

5.55

Isoelectric Point (pI)

36.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 168 - 275 1.1e-08 Leucine-rich repeat region
LRR_14 PF23598 283 - 375 2e-07 Leucine-rich repeat region
PK_Tyr_Ser-Thr PF07714 715 - 946 2.2e-21 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 715 - 946 3.4e-18 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014543)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51350
fragaria_vesca FvH4_1g01520
malus_domestica MD02G1012000.v1.1 MD15G1157800.v1.1
prunus_persica Prupe.7G253000_v2.0.a1
pyrus_communis pycom02g01130 pycom15g14160
rosa_chinensis RchiOBHm_Chr2g0086441
rosa_laevigata RLG00000015759
rosa_multiflora Rmu_sc0005127.1_g000003
rosa_roxburghii Rroxscaffold_2G00154570
rosa_rugosa Rorug01G0467300
rosa_samantha Rh2AG018100 Rh2BG017600 Rh2CG018500 Rh2DG019400
rosa_wichuraiana Rw2G001500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1995
AatII GACGTC 1 cut(s) 2117
Acc36I ACCTGC 1 cut(s) 2643
AccB1I GGYRCC 1 cut(s) 1907
AccI GTMKAC 3 cut(s) 1118, 1326, 2610
AccIII TCCGGA 1 cut(s) 278
AciI CCGC 2 cut(s) 2135, 2483
AclWI GGATC 5 cut(s) 856, 903, 919, 1279, 2177
AcoI YGGCCR 3 cut(s) 372, 415, 1620
AcsI RAATTY 5 cut(s) 1295, 1468, 2495, 2637, 2662
AcuI CTGAAG 9 cut(s) 274, 500, 725, 814, 1023, 1448, 2059, 2517, 2730
AcyI GRCGYC 1 cut(s) 2114
AfaI GTAC 6 cut(s) 83, 1394, 1983, 2300, 2366, 2539
AfiI CCNNNNNNNGG 8 cut(s) 195, 650, 1105, 1421, 1514, 2226, 2429, 2652
AjnI CCWGG 3 cut(s) 157, 369, 699
AleI CACNNNNGTG 1 cut(s) 2747
AloI GAACNNNNNNTCC 2 cut(s) 395, 427
Alw21I GWGCWC 4 cut(s) 117, 1173, 1603, 2775
Alw26I GTCTC 5 cut(s) 83, 513, 1025, 2029, 2540
AlwI GGATC 5 cut(s) 856, 903, 919, 1279, 2177
AlwNI CAGNNNCTG 3 cut(s) 74, 2540, 2570
Ama87I CYCGRG 1 cut(s) 2774
Aor13HI TCCGGA 1 cut(s) 278
AoxI GGCC 5 cut(s) 372, 415, 920, 1620, 2008
ApeKI GCWGC 7 cut(s) 59, 71, 1137, 1593, 2001, 2357, 2570
ApoI RAATTY 5 cut(s) 1295, 1468, 2495, 2637, 2662
Asp700I GAANNNNTTC 3 cut(s) 407, 2072, 2700
AspLEI GCGC 1 cut(s) 1829
AspS9I GGNCC 4 cut(s) 529, 560, 920, 1712
AsuC2I CCSGG 4 cut(s) 919, 929, 1878, 1912
AsuHPI GGTGA 5 cut(s) 358, 796, 1292, 2389, 2399
AsuII TTCGAA 1 cut(s) 1193
AvaI CYCGRG 1 cut(s) 2774
AvaII GGWCC 3 cut(s) 529, 560, 1712
AxyI CCTNAGG 1 cut(s) 1650
BaeGI GKGCMC 1 cut(s) 1430
BalI TGGCCA 2 cut(s) 374, 1622
BanI GGYRCC 1 cut(s) 1907
BanII GRGCYC 2 cut(s) 117, 1603
BarI GAAGNNNNNNTAC 2 cut(s) 1488, 1520
BauI CACGAG 1 cut(s) 1483
BbsI GAAGAC 4 cut(s) 929, 1244, 1658, 1817
Bbv12I GWGCWC 4 cut(s) 117, 1173, 1603, 2775
BbvCI CCTCAGC 1 cut(s) 501
BbvI GCAGC 7 cut(s) 71, 83, 1149, 1580, 2013, 2369, 2557
BccI CCATC 6 cut(s) 178, 1228, 1703, 2014, 2815, 2827
BceAI ACGGC 2 cut(s) 2665, 2787
BciT130I CCWGG 3 cut(s) 159, 371, 701
BciVI GTATCC 2 cut(s) 2554, 2824
BcnI CCSGG 4 cut(s) 919, 929, 1878, 1912
BcoDI GTCTC 5 cut(s) 83, 513, 1025, 2029, 2540
BfaI CTAG 9 cut(s) 242, 539, 572, 941, 1590, 1685, 1976, 2192, 2796
BfmI CTRYAG 4 cut(s) 57, 2424, 2541, 2583
BfuAI ACCTGC 1 cut(s) 2643
BfuI GTATCC 2 cut(s) 2554, 2824
BisI GCNGC 7 cut(s) 60, 72, 1138, 1594, 2002, 2358, 2571
BlpI GCTNAGC 1 cut(s) 1014
BlsI GCNGC 7 cut(s) 61, 73, 1139, 1595, 2003, 2359, 2572
BmcAI AGTACT 2 cut(s) 1394, 1983
Bme1390I CCNGG 7 cut(s) 159, 371, 701, 919, 929, 1878, 1912
Bme18I GGWCC 3 cut(s) 529, 560, 1712
BmeT110I CYCGRG 1 cut(s) 2774
BmgT120I GGNCC 4 cut(s) 529, 560, 920, 1712
BmiI GGNNCC 4 cut(s) 561, 1909, 2481, 2801
BmrFI CCNGG 7 cut(s) 159, 371, 701, 919, 929, 1878, 1912
BmrI ACTGGG 1 cut(s) 365
BmsI GCATC 9 cut(s) 454, 717, 1173, 2089, 2111, 2129, 2290, 2494, 2518
BmuI ACTGGG 1 cut(s) 365
BpiI GAAGAC 4 cut(s) 929, 1244, 1658, 1817
BpmI CTGGAG 2 cut(s) 1049, 2537
Bpu10I CCTNAGC 1 cut(s) 501
Bpu1102I GCTNAGC 1 cut(s) 1014
Bpu14I TTCGAA 1 cut(s) 1193
BpuEI CTTGAG 6 cut(s) 509, 617, 833, 1409, 1625, 2464
BpuMI CCSGG 4 cut(s) 919, 929, 1878, 1912
BsaHI GRCGYC 1 cut(s) 2114
BsaI GGTCTC 3 cut(s) 83, 2029, 2540
BsaJI CCNNGG 6 cut(s) 369, 700, 713, 2091, 2556, 2823
BsaWI WCCGGW 2 cut(s) 278, 437
BsaXI ACNNNNNCTCC 2 cut(s) 395, 425
Bsc4I CCNNNNNNNGG 8 cut(s) 195, 650, 1105, 1421, 1514, 2226, 2429, 2652
Bse118I RCCGGY 1 cut(s) 266
Bse1I ACTGG 8 cut(s) 149, 237, 360, 418, 857, 1032, 1354, 1457
Bse21I CCTNAGG 1 cut(s) 1650
Bse3DI GCAATG 1 cut(s) 2382
BseAI TCCGGA 1 cut(s) 278
BseBI CCWGG 3 cut(s) 159, 371, 701
BseDI CCNNGG 6 cut(s) 369, 700, 713, 2091, 2556, 2823
BseGI GGATG 5 cut(s) 708, 1714, 2101, 2419, 2819
BseLI CCNNNNNNNGG 8 cut(s) 195, 650, 1105, 1421, 1514, 2226, 2429, 2652
BseMI GCAATG 1 cut(s) 2382
BseNI ACTGG 8 cut(s) 149, 237, 360, 418, 857, 1032, 1354, 1457
BseRI GAGGAG 3 cut(s) 217, 1667, 2606
BseSI GKGCMC 1 cut(s) 1430
BseXI GCAGC 7 cut(s) 71, 83, 1149, 1580, 2013, 2369, 2557
BseYI CCCAGC 2 cut(s) 424, 542
BsgI GTGCAG 3 cut(s) 1422, 1962, 2776
BshFI GGCC 5 cut(s) 374, 417, 922, 1622, 2010
BshNI GGYRCC 1 cut(s) 1907
BsiHKAI GWGCWC 4 cut(s) 117, 1173, 1603, 2775
BsiHKCI CYCGRG 1 cut(s) 2774
BslFI GGGAC 4 cut(s) 1798, 1860, 2092, 2453
BslI CCNNNNNNNGG 8 cut(s) 195, 650, 1105, 1421, 1514, 2226, 2429, 2652
BsmAI GTCTC 5 cut(s) 83, 513, 1025, 2029, 2540
BsmFI GGGAC 4 cut(s) 1798, 1860, 2092, 2453
BsmI GAATGC 4 cut(s) 467, 1140, 1829, 2183
BsnI GGCC 5 cut(s) 374, 417, 922, 1622, 2010
Bso31I GGTCTC 3 cut(s) 83, 2029, 2540
BsoBI CYCGRG 1 cut(s) 2774
Bsp119I TTCGAA 1 cut(s) 1193
Bsp1286I GDGCHC 5 cut(s) 117, 1173, 1430, 1603, 2775
Bsp13I TCCGGA 1 cut(s) 278
Bsp1720I GCTNAGC 1 cut(s) 1014
Bsp19I CCATGG 3 cut(s) 2091, 2556, 2823
BspACI CCGC 2 cut(s) 2135, 2483
BspANI GGCC 5 cut(s) 374, 417, 922, 1622, 2010
BspEI TCCGGA 1 cut(s) 278
BspLI GGNNCC 4 cut(s) 561, 1909, 2481, 2801
BspMAI CTGCAG 3 cut(s) 61, 2428, 2545
BspMI ACCTGC 1 cut(s) 2643
BspPI GGATC 5 cut(s) 856, 903, 919, 1279, 2177
BspQI GCTCTTC 1 cut(s) 2063
BspT104I TTCGAA 1 cut(s) 1193
BspT107I GGYRCC 1 cut(s) 1907
BspTNI GGTCTC 3 cut(s) 83, 2029, 2540
BsrDI GCAATG 1 cut(s) 2382
BsrFI RCCGGY 1 cut(s) 266
BsrI ACTGG 8 cut(s) 149, 237, 360, 418, 857, 1032, 1354, 1457
BssAI RCCGGY 1 cut(s) 266
BssECI CCNNGG 6 cut(s) 369, 700, 713, 2091, 2556, 2823
BssNAI GTATAC 1 cut(s) 2611
BssNI GRCGYC 1 cut(s) 2114
BssSI CACGAG 1 cut(s) 1483
BssT1I CCWWGG 4 cut(s) 713, 2091, 2556, 2823
Bst1107I GTATAC 1 cut(s) 2611
Bst2BI CACGAG 1 cut(s) 1483
Bst2UI CCWGG 3 cut(s) 159, 371, 701
Bst4CI ACNGT 7 cut(s) 667, 963, 1559, 1583, 1938, 2158, 2579
Bst6I CTCTTC 5 cut(s) 251, 833, 1041, 2063, 2583
BstACI GRCGYC 1 cut(s) 2114
BstBI TTCGAA 1 cut(s) 1193
BstC8I GCNNGC 9 cut(s) 264, 347, 498, 656, 737, 1659, 1945, 2056, 2674
BstDSI CCRYGG 3 cut(s) 2091, 2556, 2823
BstF5I GGATG 5 cut(s) 708, 1714, 2101, 2419, 2819
BstHHI GCGC 1 cut(s) 1829
BstMAI GTCTC 5 cut(s) 83, 513, 1025, 2029, 2540
BstMWI GCNNNNNNNGC 6 cut(s) 68, 1177, 1970, 2007, 2682, 2799
BstNI CCWGG 3 cut(s) 159, 371, 701
BstSCI CCNGG 7 cut(s) 157, 369, 699, 917, 927, 1876, 1910
BstSFI CTRYAG 4 cut(s) 57, 2424, 2541, 2583
BstSLI GKGCMC 1 cut(s) 1430
BstV1I GCAGC 7 cut(s) 71, 83, 1149, 1580, 2013, 2369, 2557
BstV2I GAAGAC 4 cut(s) 929, 1244, 1658, 1817
BstX2I RGATCY 2 cut(s) 895, 1284
BstXI CCANNNNNNTGG 1 cut(s) 845
BstYI RGATCY 2 cut(s) 895, 1284
BstZ17I GTATAC 1 cut(s) 2611
Bsu36I CCTNAGG 1 cut(s) 1650
BsuI GTATCC 2 cut(s) 2554, 2824
BsuRI GGCC 5 cut(s) 374, 417, 922, 1622, 2010
BtgI CCRYGG 3 cut(s) 2091, 2556, 2823
BtgZI GCGATG 1 cut(s) 2073
BtsCI GGATG 5 cut(s) 708, 1714, 2101, 2419, 2819
BtsI GCAGTG 1 cut(s) 491
BveI ACCTGC 1 cut(s) 2643
Cac8I GCNNGC 9 cut(s) 264, 347, 498, 656, 737, 1659, 1945, 2056, 2674
CaiI CAGNNNCTG 3 cut(s) 74, 2540, 2570
CfoI GCGC 1 cut(s) 1829
Cfr10I RCCGGY 1 cut(s) 266
Cfr13I GGNCC 4 cut(s) 529, 560, 920, 1712
Csp6I GTAC 6 cut(s) 82, 1393, 1982, 2299, 2365, 2538
CspCI CAANNNNNGTGG 6 cut(s) 630, 665, 840, 875, 1062, 1097
CviQI GTAC 6 cut(s) 82, 1393, 1982, 2299, 2365, 2538
EaeI YGGCCR 3 cut(s) 372, 415, 1620
Eam1104I CTCTTC 5 cut(s) 251, 833, 1041, 2063, 2583
EarI CTCTTC 5 cut(s) 251, 833, 1041, 2063, 2583
Ecl136II GAGCTC 2 cut(s) 115, 1601
Eco130I CCWWGG 4 cut(s) 713, 2091, 2556, 2823
Eco24I GRGCYC 2 cut(s) 117, 1603
Eco31I GGTCTC 3 cut(s) 83, 2029, 2540
Eco32I GATATC 1 cut(s) 1153
Eco47I GGWCC 3 cut(s) 529, 560, 1712
Eco53kI GAGCTC 2 cut(s) 115, 1601
Eco57I CTGAAG 9 cut(s) 274, 500, 725, 814, 1023, 1448, 2059, 2517, 2730
Eco81I CCTNAGG 1 cut(s) 1650
Eco88I CYCGRG 1 cut(s) 2774
EcoICRI GAGCTC 2 cut(s) 115, 1601
EcoO109I RGGNCCY 1 cut(s) 529
EcoRI GAATTC 2 cut(s) 2495, 2662
EcoRII CCWGG 3 cut(s) 157, 369, 699
EcoRV GATATC 1 cut(s) 1153
EcoT14I CCWWGG 4 cut(s) 713, 2091, 2556, 2823
EcoT22I ATGCAT 2 cut(s) 469, 2104
EcoT38I GRGCYC 2 cut(s) 117, 1603
ErhI CCWWGG 4 cut(s) 713, 2091, 2556, 2823
FaqI GGGAC 4 cut(s) 1798, 1860, 2092, 2453
FauNDI CATATG 2 cut(s) 675, 1321
FblI GTMKAC 3 cut(s) 1118, 1326, 2610
Fnu4HI GCNGC 7 cut(s) 60, 72, 1138, 1594, 2002, 2358, 2571
FokI GGATG 5 cut(s) 695, 1721, 2108, 2406, 2806
FriOI GRGCYC 2 cut(s) 117, 1603
Fsp4HI GCNGC 7 cut(s) 60, 72, 1138, 1594, 2002, 2358, 2571
FspBI CTAG 9 cut(s) 242, 539, 572, 941, 1590, 1685, 1976, 2192, 2796
GlaI GCGC 1 cut(s) 1828
GluI GCNGC 7 cut(s) 60, 72, 1138, 1594, 2002, 2358, 2571
GsaI CCCAGC 2 cut(s) 428, 546
GsuI CTGGAG 2 cut(s) 1049, 2537
HaeIII GGCC 5 cut(s) 374, 417, 922, 1622, 2010
HhaI GCGC 1 cut(s) 1829
Hin1I GRCGYC 1 cut(s) 2114
Hin6I GCGC 1 cut(s) 1827
HinP1I GCGC 1 cut(s) 1827
HincII GTYRAC 2 cut(s) 286, 2658
HindII GTYRAC 2 cut(s) 286, 2658
HindIII AAGCTT 8 cut(s) 385, 809, 889, 1385, 1744, 1916, 2674, 2840
HphI GGTGA 5 cut(s) 358, 796, 1292, 2389, 2399
Hpy166II GTNNAC 7 cut(s) 286, 366, 872, 1119, 1327, 2611, 2658
Hpy8I GTNNAC 7 cut(s) 286, 366, 872, 1119, 1327, 2611, 2658
Hpy99I CGWCG 1 cut(s) 2116
HpyAV CCTTC 6 cut(s) 28, 104, 998, 1032, 1731, 2414
HpyCH4III ACNGT 7 cut(s) 667, 963, 1559, 1583, 1938, 2158, 2579
HpyCH4IV ACGT 1 cut(s) 2114
HpyF10VI GCNNNNNNNGC 6 cut(s) 68, 1177, 1970, 2007, 2682, 2799
HpySE526I ACGT 1 cut(s) 2114
Hsp92I GRCGYC 1 cut(s) 2114
HspAI GCGC 1 cut(s) 1827
Kpn2I TCCGGA 1 cut(s) 278
LguI GCTCTTC 1 cut(s) 2063
LmnI GCTCC 7 cut(s) 354, 546, 1384, 1654, 2593, 2669, 2799
Lsp1109I GCAGC 7 cut(s) 71, 83, 1149, 1580, 2013, 2369, 2557
LweI GCATC 9 cut(s) 454, 717, 1173, 2089, 2111, 2129, 2290, 2494, 2518
MaeI CTAG 9 cut(s) 242, 539, 572, 941, 1590, 1685, 1976, 2192, 2796
MaeII ACGT 1 cut(s) 2114
MaeIII GTNAC 5 cut(s) 232, 326, 489, 667, 816
MfeI CAATTG 2 cut(s) 719, 1231
MflI RGATCY 2 cut(s) 895, 1284
MhlI GDGCHC 5 cut(s) 117, 1173, 1430, 1603, 2775
MlsI TGGCCA 2 cut(s) 374, 1622
MluNI TGGCCA 2 cut(s) 374, 1622
MlyI GAGTC 7 cut(s) 291, 664, 785, 824, 976, 1027, 2031
MmeI TCCRAC 3 cut(s) 1119, 1816, 2670
Mox20I TGGCCA 2 cut(s) 374, 1622
Mph1103I ATGCAT 2 cut(s) 469, 2104
MroI TCCGGA 1 cut(s) 278
MroXI GAANNNNTTC 3 cut(s) 407, 2072, 2700
MscI TGGCCA 2 cut(s) 374, 1622
MseI TTAA 5 cut(s) 104, 380, 1176, 1398, 2837
MslI CAYNNNNRTG 4 cut(s) 1444, 1952, 2125, 2747
Msp20I TGGCCA 2 cut(s) 374, 1622
MspA1I CMGCKG 3 cut(s) 62, 74, 428
MspR9I CCNGG 7 cut(s) 159, 371, 701, 919, 929, 1878, 1912
MunI CAATTG 2 cut(s) 719, 1231
Mva1269I GAATGC 4 cut(s) 467, 1140, 1829, 2183
MvaI CCWGG 3 cut(s) 159, 371, 701
MwoI GCNNNNNNNGC 6 cut(s) 68, 1177, 1970, 2007, 2682, 2799
NciI CCSGG 4 cut(s) 919, 929, 1878, 1912
NcoI CCATGG 3 cut(s) 2091, 2556, 2823
NdeI CATATG 2 cut(s) 675, 1321
NlaIV GGNNCC 4 cut(s) 561, 1909, 2481, 2801
NmeAIII GCCGAG 1 cut(s) 1622
NmuCI GTSAC 3 cut(s) 489, 667, 816
NsiI ATGCAT 2 cut(s) 469, 2104
NspV TTCGAA 1 cut(s) 1193
OliI CACNNNNGTG 1 cut(s) 2747
PaeR7I CTCGAG 1 cut(s) 2774
PciSI GCTCTTC 1 cut(s) 2063
PcsI WCGNNNNNNNCGW 1 cut(s) 2111
PctI GAATGC 4 cut(s) 467, 1140, 1829, 2183
PdmI GAANNNNTTC 3 cut(s) 407, 2072, 2700
PfoI TCCNGGA 2 cut(s) 157, 927
PkrI GCNGC 7 cut(s) 61, 73, 1139, 1595, 2003, 2359, 2572
PleI GAGTC 7 cut(s) 290, 664, 784, 823, 975, 1027, 2031
PpsI GAGTC 7 cut(s) 290, 664, 784, 823, 975, 1027, 2031
PpuMI RGGWCCY 1 cut(s) 529
PsiI TTATAA 1 cut(s) 1995
Psp124BI GAGCTC 2 cut(s) 117, 1603
Psp5II RGGWCCY 1 cut(s) 529
Psp6I CCWGG 3 cut(s) 157, 369, 699
PspFI CCCAGC 2 cut(s) 424, 542
PspGI CCWGG 3 cut(s) 157, 369, 699
PspN4I GGNNCC 4 cut(s) 561, 1909, 2481, 2801
PspPI GGNCC 4 cut(s) 529, 560, 920, 1712
PspPPI RGGWCCY 1 cut(s) 529
PspXI VCTCGAGB 1 cut(s) 2774
PsrI GAACNNNNNNTAC 2 cut(s) 311, 343
PstI CTGCAG 3 cut(s) 61, 2428, 2545
PstNI CAGNNNCTG 3 cut(s) 74, 2540, 2570
PsuI RGATCY 2 cut(s) 895, 1284
PvuII CAGCTG 3 cut(s) 62, 74, 428
RsaI GTAC 6 cut(s) 83, 1394, 1983, 2300, 2366, 2539
RsaNI GTAC 6 cut(s) 82, 1393, 1982, 2299, 2365, 2538
RseI CAYNNNNRTG 4 cut(s) 1444, 1952, 2125, 2747
SacI GAGCTC 2 cut(s) 117, 1603
SapI GCTCTTC 1 cut(s) 2063
SaqAI TTAA 5 cut(s) 104, 380, 1176, 1398, 2837
SatI GCNGC 7 cut(s) 60, 72, 1138, 1594, 2002, 2358, 2571
Sau96I GGNCC 4 cut(s) 529, 560, 920, 1712
ScaI AGTACT 2 cut(s) 1394, 1983
SchI GAGTC 7 cut(s) 291, 664, 785, 824, 976, 1027, 2031
ScrFI CCNGG 7 cut(s) 159, 371, 701, 919, 929, 1878, 1912
SduI GDGCHC 5 cut(s) 117, 1173, 1430, 1603, 2775
SfaNI GCATC 9 cut(s) 454, 717, 1173, 2089, 2111, 2129, 2290, 2494, 2518
SfcI CTRYAG 4 cut(s) 57, 2424, 2541, 2583
Sfr274I CTCGAG 1 cut(s) 2774
SfuI TTCGAA 1 cut(s) 1193
SinI GGWCC 3 cut(s) 529, 560, 1712
SlaI CTCGAG 1 cut(s) 2774
SmiMI CAYNNNNRTG 4 cut(s) 1444, 1952, 2125, 2747
SmlI CTYRAG 7 cut(s) 524, 596, 812, 1388, 1604, 2443, 2774
SmoI CTYRAG 7 cut(s) 524, 596, 812, 1388, 1604, 2443, 2774
SsiI CCGC 2 cut(s) 2135, 2483
SspMI CTAG 9 cut(s) 242, 539, 572, 941, 1590, 1685, 1976, 2192, 2796
SstI GAGCTC 2 cut(s) 117, 1603
StyD4I CCNGG 7 cut(s) 157, 369, 699, 917, 927, 1876, 1910
StyI CCWWGG 4 cut(s) 713, 2091, 2556, 2823
TaaI ACNGT 7 cut(s) 667, 963, 1559, 1583, 1938, 2158, 2579
TaiI ACGT 1 cut(s) 2117
TaqI TCGA 5 cut(s) 1193, 2690, 2775, 2815, 2854
TatI WGTACW 4 cut(s) 81, 1392, 1981, 2364
Tru1I TTAA 5 cut(s) 104, 380, 1176, 1398, 2837
Tru9I TTAA 5 cut(s) 104, 380, 1176, 1398, 2837
TseFI GTSAC 3 cut(s) 489, 667, 816
TseI GCWGC 7 cut(s) 59, 71, 1137, 1593, 2001, 2357, 2570
Tsp45I GTSAC 3 cut(s) 489, 667, 816
TspDTI ATGAA 8 cut(s) 37, 596, 924, 1308, 1568, 2203, 2427, 2742
TspGWI ACGGA 1 cut(s) 2331
VpaK11BI GGWCC 3 cut(s) 529, 560, 1712
XapI RAATTY 5 cut(s) 1295, 1468, 2495, 2637, 2662
XbaI TCTAGA 2 cut(s) 241, 940
XcmI CCANNNNNNNNNTGG 2 cut(s) 425, 2089
XhoI CTCGAG 1 cut(s) 2774
XmiI GTMKAC 3 cut(s) 1118, 1326, 2610
XmnI GAANNNNTTC 3 cut(s) 407, 2072, 2700
XspI CTAG 9 cut(s) 242, 539, 572, 941, 1590, 1685, 1976, 2192, 2796
ZraI GACGTC 1 cut(s) 2115
ZrmI AGTACT 2 cut(s) 1394, 1983
Zsp2I ATGCAT 2 cut(s) 469, 2104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.