Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2A

Orthogroup ID

OG0014543

Physical Location & Seq

Genomic Coordinates Reverse (-)

1374288 .. 1377623

Gene Structure 3336 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2AG018100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2856 bp

ATGGAGATTTCCCACTCCTTCTTCTACAATCTTCTTCTCTTGTTCATGTTCTCAGCAGCTGTTTCAGCAGCTGATCTTTACTCAGAGACCCTTCTGAGCTTAAAGTCTGAGCTCACAGATGACAATGATAGCTTACAAGACTGGTCTGTGCCTCCTGGACAAAACCCATCTGCGAAAATCTATGCCTGTTCTTGGTCTGGTGTCAAATGCAACAGCAACAACTCCTCTGTTACCAGTCTAGACCTGTCAGTGAAGAGTCTTGCCGGTTCAATCTCCGGAGTCAACTTCAGTGTCTTTACTGATCTTCTTGACCTGAACCTGAGTTACAACTCATTTTCCGGCAAGCTCCCACTGGGAATGTTCACCCTGGCCAACTTAACAAGCTTGGACATTAGCCGGAACAACTTCTCCGGCCAGTTCCCAGCTGGAATCTCCGGTCTTACCAATCTGGTTGTGTTTGATGCATTCAGCAACAGCTTTTCTGGGTCACTGCCAGCTGAGGTGTCTCAACTTCAGAATCTCAAGGTCCTGAATCTAGCTGGGAGCTACTTCAAGGGTCCAATCCCACTAGAATATGGTAATTTTCAAAGCCTTGAGTTTATTCATCTTGCAGGGAATATGCTCAGTGGGAACATACCACCTGAATTGGGCAAGCTCAAAACAGTGACTCATATGGAGATTGGCTACAATTCTTACCAGGGAGGCATCCCTTGGCAATTGGGAAACATGAGCCAGCTTCAGTATCTTGATATTGCTAATGCAAATCTCTCTGAGTCTATTCCAAGAGAGCTTGGGAATCTCACCAAGCTTGAGTCTCTCTTTCTCTTCAGAAACCAGCTTAGTGGATCAATACCAGTGGAGTTCAGTAAACTTCAATCTCTCACAAGCTTGGATCTTTCGGATAATGAAATCTCCGGGCCGATCCCGGATAGTCTTCTAGAGTTGAAGAACCTGAAACTGTTGAGTCTTTTCTACAATGAGATGAGTGGTGCTGTTCCTGAAGGGATTGCTGAGCTTCCTTCACTGGAGACTCTTCTTATATGGAACAACTACTTCTCTGGGAATCTTCCACAGGATTTGGGGAGGAACTCCAACCTCATGTGGGTTGATGTGTCTACAAACAACTTCAATGGCAGCATTCCAGCAGATATCTGTGTGAAAGGTGTGCTCTTTAAGCTGATGATGTTTTCGAATAATTTTAGTGGCAGTCTTGCACCATCTCTGTCCAATTGTTCTTCACTTGTTCGTCTCCGGCTTGAAGATAACTCATTCTCAGGTGAGATCCCTTCGAAATTCAGCAACTTTCGTGATCTTTCATATGTAGACTTGTCCAAGAATAAACTTACTGGTGGGATTCCAATAGATATAGACCAAGCTCCCAAGCTTGAGTACTTTAATGTGTCCAATAATCCAGAATTAGGGGGCACAATTCCTGCACAAACATGGTCTTTGCCAGTTCTTCAGAATTTCTCAGCTTCCTCGTGTGGTATCGTAGGCAATCTTCCGGCATTTGGAAGCTGTAAATCCTTATCTGTTATTGAATTGAACATGAACAGTTTATCAGGGGGTGTTCCAGACAGTGTTTCTAGCTGCCGAGCTCTTGAGAGATTTGGTTTGGCCAACAATAACTTATCAGGTCATATACCTGAGGAGCTTGCAAGTCTTCCTGCTCTGCGTGTCCTAGACCTATCACATAATAGTTTGGATGGACCAATACCTTCAAAGTTTGGTTCTTCTTCAAGCTTATCACTTCTGAATGTGTCTTTCAATGATATTTCTGGGACAATTCCCTCTGGAAAGGTAGTGAAGACTATGGGAAGTAGCGCATTCGTTGGAAATCCAAAGCTATGTGGAGAGCCTTTGAGATCATGTCCCGGTTCAGTGGCTATCTTCGGAAGCAGAGGCACCGGGAAGCTTATATGGGTCTTGCTACTGTGTGCAGGCATAGTTATGTTCCTTGCTCTGTCAGCTCTAGGAGTACTTTATTTTTATAAAGGCAGCAAAGGCCAATGGAAGACGGTCTCATTTGCTGGACTCCCTCAATTCACAGCAAGCGATGTATTGAAGAGCTTCAGTTCCATAGAATCCATGGATGATGCATTGTCCCCGACGTCAGCATCAGTATGCAAAGCGGTTCTGCCAACAGGAATCACAGTTTCAGTGAAGAAGTTTGAATGCGATCCGAAGCTAGTGAATGTTATGTTGCCATTCATAACCCGAATTGGGAGTGCAAGGCATAAGAATCTAATCCGATTGCTCGGATTTTGCTACAACAAGCATCTGGTTTATCTCTTGTACGATTACTTGCCCAATGGAAGTTTATCAGAAAATATAAGAGTGAAAAGGGAATGGGCAGCTAAGTACAAAATTGTGGTGAGCATTGCAAGGGGATTGTGCTTCCTTCACCATGAATGTCATCCTGCAATTGGTCATGGAGACTTGAGATCAAGTAATATAATGTTTGACGAAAACATGGAACCGCATCTGACAGAATTCGGATTCAAGCATCTGCTGGAGTTGAACAAAACTTCAGGTACTGCAGAGACCTCCACCATGGATACAGGTGCTGCAAACAGTTCTGTAGAAGAGGAGCTATACAGAGATGTATACAGCTTCGGGGAGATCATGCTCGAAATTTTATCCAACGGCAGGTTGACGAATTCAGGAGCAAGCTTACATAGCAAGTCGAGGGAAAGTGTTCTCAGAGAAATAATCAATGAGAATGAAGTTGGTTCCAACACTTCAGTGCAACAGGAGATAAAAAGGGTGCTCGAGGTAGCTACGCTCTGCACTAGGAGCCGACCATCTGATCGACCATCCATGGAGGATACATTAAAGCTTTTACAGGCTCGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001763 GO:0003006 GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009987 GO:0010016 GO:0010065 GO:0010067 GO:0010087 GO:0010089 GO:0010154 GO:0010223 GO:0010346 GO:0016020 GO:0022414 GO:0032501 GO:0032502 GO:0044464 GO:0048316 GO:0048367 GO:0048507 GO:0048508 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0051301 GO:0061458 GO:0071944 GO:1905393

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

951

Amino Acids

103.19

Weight (kDa)

5.54

Isoelectric Point (pI)

35.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	29 - 71	8.1e-06	Leucine rich repeat N-terminal domain
LRR_14	PF23598	167 - 275	4.4e-09	Leucine-rich repeat region
LRR_14	PF23598	282 - 376	1.2e-07	Leucine-rich repeat region
Pkinase	PF00069	714 - 945	1.4e-18	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	715 - 945	3.1e-21	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014543)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G51350
fragaria_vesca	FvH4_1g01520
malus_domestica	MD02G1012000.v1.1 MD15G1157800.v1.1
prunus_persica	Prupe.7G253000_v2.0.a1
pyrus_communis	pycom02g01130 pycom15g14160
rosa_chinensis	RchiOBHm_Chr2g0086441
rosa_laevigata	RLG00000015759
rosa_multiflora	Rmu_sc0005127.1_g000003
rosa_roxburghii	Rroxscaffold_2G00154570
rosa_rugosa	Rorug01G0467300
rosa_samantha	Rh2AG018100 Rh2BG017600 Rh2CG018500 Rh2DG019400
rosa_wichuraiana	Rw2G001500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1992
AatII	GACGTC	1 cut(s)	2114
Acc36I	ACCTGC	1 cut(s)	2640
AccB1I	GGYRCC	1 cut(s)	1904
AccI	GTMKAC	3 cut(s)	1115, 1323, 2607
AccIII	TCCGGA	1 cut(s)	275
AciI	CCGC	2 cut(s)	2132, 2480
AclWI	GGATC	5 cut(s)	853, 900, 916, 1276, 2174
AcoI	YGGCCR	3 cut(s)	369, 412, 1617
AcsI	RAATTY	5 cut(s)	1292, 1465, 2492, 2634, 2659
AcuI	CTGAAG	9 cut(s)	271, 497, 722, 811, 1020, 1445, 2056, 2514, 2727
AcyI	GRCGYC	1 cut(s)	2111
AfaI	GTAC	5 cut(s)	1391, 1980, 2297, 2363, 2536
AfiI	CCNNNNNNNGG	8 cut(s)	192, 647, 1102, 1418, 1511, 2223, 2426, 2649
AjnI	CCWGG	3 cut(s)	154, 366, 696
AleI	CACNNNNGTG	1 cut(s)	2744
AloI	GAACNNNNNNTCC	2 cut(s)	392, 424
Alw21I	GWGCWC	4 cut(s)	114, 1170, 1600, 2772
Alw26I	GTCTC	8 cut(s)	80, 510, 819, 1022, 1253, 2026, 2430, 2537
AlwI	GGATC	5 cut(s)	853, 900, 916, 1276, 2174
AlwNI	CAGNNNCTG	4 cut(s)	59, 71, 2537, 2567
Ama87I	CYCGRG	1 cut(s)	2771
Aor13HI	TCCGGA	1 cut(s)	275
AoxI	GGCC	5 cut(s)	369, 412, 917, 1617, 2005
ApeKI	GCWGC	7 cut(s)	56, 68, 1134, 1590, 1998, 2354, 2567
ApoI	RAATTY	5 cut(s)	1292, 1465, 2492, 2634, 2659
Asp700I	GAANNNNTTC	3 cut(s)	404, 2069, 2697
AspLEI	GCGC	1 cut(s)	1826
AspS9I	GGNCC	4 cut(s)	526, 557, 917, 1709
AsuC2I	CCSGG	4 cut(s)	916, 926, 1875, 1909
AsuHPI	GGTGA	5 cut(s)	355, 793, 1289, 2386, 2396
AsuII	TTCGAA	2 cut(s)	1190, 1289
AvaI	CYCGRG	1 cut(s)	2771
AvaII	GGWCC	3 cut(s)	526, 557, 1709
AxyI	CCTNAGG	1 cut(s)	1647
BaeGI	GKGCMC	1 cut(s)	1427
BalI	TGGCCA	2 cut(s)	371, 1619
BanI	GGYRCC	1 cut(s)	1904
BanII	GRGCYC	2 cut(s)	114, 1600
BarI	GAAGNNNNNNTAC	2 cut(s)	1485, 1517
BauI	CACGAG	1 cut(s)	1480
BbsI	GAAGAC	4 cut(s)	926, 1655, 1814, 2021
Bbv12I	GWGCWC	4 cut(s)	114, 1170, 1600, 2772
BbvCI	CCTCAGC	1 cut(s)	498
BbvI	GCAGC	7 cut(s)	68, 80, 1146, 1577, 2010, 2366, 2554
BccI	CCATC	5 cut(s)	175, 1225, 1700, 2812, 2824
BceAI	ACGGC	1 cut(s)	2662
BciT130I	CCWGG	3 cut(s)	156, 368, 698
BciVI	GTATCC	2 cut(s)	2551, 2821
BcnI	CCSGG	4 cut(s)	916, 926, 1875, 1909
BcoDI	GTCTC	8 cut(s)	80, 510, 819, 1022, 1253, 2026, 2430, 2537
BfaI	CTAG	9 cut(s)	239, 536, 569, 938, 1587, 1682, 1973, 2189, 2793
BfmI	CTRYAG	2 cut(s)	2538, 2580
BfuAI	ACCTGC	1 cut(s)	2640
BfuI	GTATCC	2 cut(s)	2551, 2821
BisI	GCNGC	7 cut(s)	57, 69, 1135, 1591, 1999, 2355, 2568
BlpI	GCTNAGC	1 cut(s)	1011
BlsI	GCNGC	7 cut(s)	58, 70, 1136, 1592, 2000, 2356, 2569
BmcAI	AGTACT	2 cut(s)	1391, 1980
Bme1390I	CCNGG	7 cut(s)	156, 368, 698, 916, 926, 1875, 1909
Bme18I	GGWCC	3 cut(s)	526, 557, 1709
BmeT110I	CYCGRG	1 cut(s)	2771
BmgT120I	GGNCC	4 cut(s)	526, 557, 917, 1709
BmiI	GGNNCC	5 cut(s)	558, 1906, 2478, 2734, 2798
BmrFI	CCNGG	7 cut(s)	156, 368, 698, 916, 926, 1875, 1909
BmrI	ACTGGG	1 cut(s)	362
BmsI	GCATC	7 cut(s)	451, 714, 2086, 2126, 2287, 2491, 2515
BmuI	ACTGGG	1 cut(s)	362
BpiI	GAAGAC	4 cut(s)	926, 1655, 1814, 2021
BpmI	CTGGAG	2 cut(s)	1046, 2534
Bpu10I	CCTNAGC	1 cut(s)	498
Bpu1102I	GCTNAGC	1 cut(s)	1011
Bpu14I	TTCGAA	2 cut(s)	1190, 1289
BpuEI	CTTGAG	6 cut(s)	506, 614, 830, 1406, 1622, 2461
BpuMI	CCSGG	4 cut(s)	916, 926, 1875, 1909
BsaHI	GRCGYC	1 cut(s)	2111
BsaI	GGTCTC	3 cut(s)	80, 2026, 2537
BsaJI	CCNNGG	6 cut(s)	366, 697, 710, 2088, 2553, 2820
BsaWI	WCCGGW	2 cut(s)	275, 434
BsaXI	ACNNNNNCTCC	2 cut(s)	392, 422
Bsc4I	CCNNNNNNNGG	8 cut(s)	192, 647, 1102, 1418, 1511, 2223, 2426, 2649
Bse118I	RCCGGY	1 cut(s)	263
Bse1I	ACTGG	8 cut(s)	146, 234, 357, 415, 854, 1029, 1351, 1454
Bse21I	CCTNAGG	1 cut(s)	1647
Bse3DI	GCAATG	1 cut(s)	2379
BseAI	TCCGGA	1 cut(s)	275
BseBI	CCWGG	3 cut(s)	156, 368, 698
BseDI	CCNNGG	6 cut(s)	366, 697, 710, 2088, 2553, 2820
BseGI	GGATG	5 cut(s)	705, 1711, 2098, 2416, 2816
BseLI	CCNNNNNNNGG	8 cut(s)	192, 647, 1102, 1418, 1511, 2223, 2426, 2649
BseMI	GCAATG	1 cut(s)	2379
BseNI	ACTGG	8 cut(s)	146, 234, 357, 415, 854, 1029, 1351, 1454
BseRI	GAGGAG	3 cut(s)	214, 1664, 2603
BseSI	GKGCMC	1 cut(s)	1427
BseXI	GCAGC	7 cut(s)	68, 80, 1146, 1577, 2010, 2366, 2554
BseYI	CCCAGC	2 cut(s)	421, 539
BsgI	GTGCAG	3 cut(s)	1419, 1959, 2773
BshFI	GGCC	5 cut(s)	371, 414, 919, 1619, 2007
BshNI	GGYRCC	1 cut(s)	1904
BsiHKAI	GWGCWC	4 cut(s)	114, 1170, 1600, 2772
BsiHKCI	CYCGRG	1 cut(s)	2771
BslFI	GGGAC	3 cut(s)	1795, 1857, 2089
BslI	CCNNNNNNNGG	8 cut(s)	192, 647, 1102, 1418, 1511, 2223, 2426, 2649
BsmAI	GTCTC	8 cut(s)	80, 510, 819, 1022, 1253, 2026, 2430, 2537
BsmBI	CGTCTC	1 cut(s)	1253
BsmFI	GGGAC	3 cut(s)	1795, 1857, 2089
BsmI	GAATGC	4 cut(s)	464, 1137, 1826, 2180
BsnI	GGCC	5 cut(s)	371, 414, 919, 1619, 2007
Bso31I	GGTCTC	3 cut(s)	80, 2026, 2537
BsoBI	CYCGRG	1 cut(s)	2771
Bsp119I	TTCGAA	2 cut(s)	1190, 1289
Bsp1286I	GDGCHC	5 cut(s)	114, 1170, 1427, 1600, 2772
Bsp13I	TCCGGA	1 cut(s)	275
Bsp1720I	GCTNAGC	1 cut(s)	1011
Bsp19I	CCATGG	3 cut(s)	2088, 2553, 2820
BspACI	CCGC	2 cut(s)	2132, 2480
BspANI	GGCC	5 cut(s)	371, 414, 919, 1619, 2007
BspEI	TCCGGA	1 cut(s)	275
BspLI	GGNNCC	5 cut(s)	558, 1906, 2478, 2734, 2798
BspMAI	CTGCAG	1 cut(s)	2542
BspMI	ACCTGC	1 cut(s)	2640
BspPI	GGATC	5 cut(s)	853, 900, 916, 1276, 2174
BspQI	GCTCTTC	1 cut(s)	2060
BspT104I	TTCGAA	2 cut(s)	1190, 1289
BspT107I	GGYRCC	1 cut(s)	1904
BspTNI	GGTCTC	3 cut(s)	80, 2026, 2537
BsrDI	GCAATG	1 cut(s)	2379
BsrFI	RCCGGY	1 cut(s)	263
BsrI	ACTGG	8 cut(s)	146, 234, 357, 415, 854, 1029, 1351, 1454
BssAI	RCCGGY	1 cut(s)	263
BssECI	CCNNGG	6 cut(s)	366, 697, 710, 2088, 2553, 2820
BssNAI	GTATAC	1 cut(s)	2608
BssNI	GRCGYC	1 cut(s)	2111
BssSI	CACGAG	1 cut(s)	1480
BssT1I	CCWWGG	4 cut(s)	710, 2088, 2553, 2820
Bst1107I	GTATAC	1 cut(s)	2608
Bst2BI	CACGAG	1 cut(s)	1480
Bst2UI	CCWGG	3 cut(s)	156, 368, 698
Bst4CI	ACNGT	8 cut(s)	664, 960, 1556, 1580, 1935, 2020, 2155, 2576
Bst6I	CTCTTC	5 cut(s)	248, 830, 1038, 2060, 2580
BstACI	GRCGYC	1 cut(s)	2111
BstBI	TTCGAA	2 cut(s)	1190, 1289
BstC8I	GCNNGC	8 cut(s)	344, 495, 653, 734, 1656, 1942, 2053, 2671
BstDSI	CCRYGG	3 cut(s)	2088, 2553, 2820
BstF5I	GGATG	5 cut(s)	705, 1711, 2098, 2416, 2816
BstHHI	GCGC	1 cut(s)	1826
BstMAI	GTCTC	8 cut(s)	80, 510, 819, 1022, 1253, 2026, 2430, 2537
BstMWI	GCNNNNNNNGC	6 cut(s)	65, 1174, 1967, 2004, 2679, 2796
BstNI	CCWGG	3 cut(s)	156, 368, 698
BstSCI	CCNGG	7 cut(s)	154, 366, 696, 914, 924, 1873, 1907
BstSFI	CTRYAG	2 cut(s)	2538, 2580
BstSLI	GKGCMC	1 cut(s)	1427
BstV1I	GCAGC	7 cut(s)	68, 80, 1146, 1577, 2010, 2366, 2554
BstV2I	GAAGAC	4 cut(s)	926, 1655, 1814, 2021
BstX2I	RGATCY	2 cut(s)	892, 1281
BstXI	CCANNNNNNTGG	1 cut(s)	842
BstYI	RGATCY	2 cut(s)	892, 1281
BstZ17I	GTATAC	1 cut(s)	2608
Bsu36I	CCTNAGG	1 cut(s)	1647
BsuI	GTATCC	2 cut(s)	2551, 2821
BsuRI	GGCC	5 cut(s)	371, 414, 919, 1619, 2007
BtgI	CCRYGG	3 cut(s)	2088, 2553, 2820
BtgZI	GCGATG	1 cut(s)	2070
BtsCI	GGATG	5 cut(s)	705, 1711, 2098, 2416, 2816
BtsI	GCAGTG	1 cut(s)	488
BveI	ACCTGC	1 cut(s)	2640
Cac8I	GCNNGC	8 cut(s)	344, 495, 653, 734, 1656, 1942, 2053, 2671
CaiI	CAGNNNCTG	4 cut(s)	59, 71, 2537, 2567
CfoI	GCGC	1 cut(s)	1826
Cfr10I	RCCGGY	1 cut(s)	263
Cfr13I	GGNCC	4 cut(s)	526, 557, 917, 1709
Csp6I	GTAC	5 cut(s)	1390, 1979, 2296, 2362, 2535
CspCI	CAANNNNNGTGG	6 cut(s)	627, 662, 837, 872, 1059, 1094
CviQI	GTAC	5 cut(s)	1390, 1979, 2296, 2362, 2535
EaeI	YGGCCR	3 cut(s)	369, 412, 1617
Eam1104I	CTCTTC	5 cut(s)	248, 830, 1038, 2060, 2580
EarI	CTCTTC	5 cut(s)	248, 830, 1038, 2060, 2580
Ecl136II	GAGCTC	2 cut(s)	112, 1598
Eco130I	CCWWGG	4 cut(s)	710, 2088, 2553, 2820
Eco24I	GRGCYC	2 cut(s)	114, 1600
Eco31I	GGTCTC	3 cut(s)	80, 2026, 2537
Eco32I	GATATC	1 cut(s)	1150
Eco47I	GGWCC	3 cut(s)	526, 557, 1709
Eco53kI	GAGCTC	2 cut(s)	112, 1598
Eco57I	CTGAAG	9 cut(s)	271, 497, 722, 811, 1020, 1445, 2056, 2514, 2727
Eco81I	CCTNAGG	1 cut(s)	1647
Eco88I	CYCGRG	1 cut(s)	2771
EcoICRI	GAGCTC	2 cut(s)	112, 1598
EcoO109I	RGGNCCY	1 cut(s)	526
EcoRI	GAATTC	2 cut(s)	2492, 2659
EcoRII	CCWGG	3 cut(s)	154, 366, 696
EcoRV	GATATC	1 cut(s)	1150
EcoT14I	CCWWGG	4 cut(s)	710, 2088, 2553, 2820
EcoT22I	ATGCAT	2 cut(s)	466, 2101
EcoT38I	GRGCYC	2 cut(s)	114, 1600
ErhI	CCWWGG	4 cut(s)	710, 2088, 2553, 2820
Esp3I	CGTCTC	1 cut(s)	1253
FaqI	GGGAC	3 cut(s)	1795, 1857, 2089
FauNDI	CATATG	2 cut(s)	672, 1318
FblI	GTMKAC	3 cut(s)	1115, 1323, 2607
Fnu4HI	GCNGC	7 cut(s)	57, 69, 1135, 1591, 1999, 2355, 2568
FokI	GGATG	5 cut(s)	692, 1718, 2105, 2403, 2803
FriOI	GRGCYC	2 cut(s)	114, 1600
Fsp4HI	GCNGC	7 cut(s)	57, 69, 1135, 1591, 1999, 2355, 2568
FspBI	CTAG	9 cut(s)	239, 536, 569, 938, 1587, 1682, 1973, 2189, 2793
GlaI	GCGC	1 cut(s)	1825
GluI	GCNGC	7 cut(s)	57, 69, 1135, 1591, 1999, 2355, 2568
GsaI	CCCAGC	2 cut(s)	425, 543
GsuI	CTGGAG	2 cut(s)	1046, 2534
HaeIII	GGCC	5 cut(s)	371, 414, 919, 1619, 2007
HhaI	GCGC	1 cut(s)	1826
Hin1I	GRCGYC	1 cut(s)	2111
Hin6I	GCGC	1 cut(s)	1824
HinP1I	GCGC	1 cut(s)	1824
HincII	GTYRAC	2 cut(s)	283, 2655
HindII	GTYRAC	2 cut(s)	283, 2655
HindIII	AAGCTT	8 cut(s)	382, 806, 886, 1382, 1741, 1913, 2671, 2837
HphI	GGTGA	5 cut(s)	355, 793, 1289, 2386, 2396
Hpy166II	GTNNAC	7 cut(s)	283, 363, 869, 1116, 1324, 2608, 2655
Hpy8I	GTNNAC	7 cut(s)	283, 363, 869, 1116, 1324, 2608, 2655
Hpy99I	CGWCG	1 cut(s)	2113
HpyAV	CCTTC	7 cut(s)	28, 101, 995, 1029, 1296, 1728, 2411
HpyCH4III	ACNGT	8 cut(s)	664, 960, 1556, 1580, 1935, 2020, 2155, 2576
HpyCH4IV	ACGT	1 cut(s)	2111
HpyF10VI	GCNNNNNNNGC	6 cut(s)	65, 1174, 1967, 2004, 2679, 2796
HpySE526I	ACGT	1 cut(s)	2111
Hsp92I	GRCGYC	1 cut(s)	2111
HspAI	GCGC	1 cut(s)	1824
Kpn2I	TCCGGA	1 cut(s)	275
LguI	GCTCTTC	1 cut(s)	2060
LmnI	GCTCC	7 cut(s)	351, 543, 1381, 1651, 2590, 2666, 2796
Lsp1109I	GCAGC	7 cut(s)	68, 80, 1146, 1577, 2010, 2366, 2554
LweI	GCATC	7 cut(s)	451, 714, 2086, 2126, 2287, 2491, 2515
MaeI	CTAG	9 cut(s)	239, 536, 569, 938, 1587, 1682, 1973, 2189, 2793
MaeII	ACGT	1 cut(s)	2111
MaeIII	GTNAC	4 cut(s)	229, 323, 486, 664
MfeI	CAATTG	3 cut(s)	716, 1228, 2424
MflI	RGATCY	2 cut(s)	892, 1281
MhlI	GDGCHC	5 cut(s)	114, 1170, 1427, 1600, 2772
MlsI	TGGCCA	2 cut(s)	371, 1619
MluNI	TGGCCA	2 cut(s)	371, 1619
MlyI	GAGTC	8 cut(s)	265, 288, 661, 782, 821, 973, 1024, 2028
MmeI	TCCRAC	4 cut(s)	1116, 1813, 2667, 2760
Mox20I	TGGCCA	2 cut(s)	371, 1619
Mph1103I	ATGCAT	2 cut(s)	466, 2101
MroI	TCCGGA	1 cut(s)	275
MroXI	GAANNNNTTC	3 cut(s)	404, 2069, 2697
MscI	TGGCCA	2 cut(s)	371, 1619
MseI	TTAA	5 cut(s)	101, 377, 1173, 1395, 2834
MslI	CAYNNNNRTG	4 cut(s)	1441, 1949, 2122, 2744
Msp20I	TGGCCA	2 cut(s)	371, 1619
MspA1I	CMGCKG	4 cut(s)	59, 71, 425, 497
MspR9I	CCNGG	7 cut(s)	156, 368, 698, 916, 926, 1875, 1909
MunI	CAATTG	3 cut(s)	716, 1228, 2424
Mva1269I	GAATGC	4 cut(s)	464, 1137, 1826, 2180
MvaI	CCWGG	3 cut(s)	156, 368, 698
MwoI	GCNNNNNNNGC	6 cut(s)	65, 1174, 1967, 2004, 2679, 2796
NciI	CCSGG	4 cut(s)	916, 926, 1875, 1909
NcoI	CCATGG	3 cut(s)	2088, 2553, 2820
NdeI	CATATG	2 cut(s)	672, 1318
NlaIV	GGNNCC	5 cut(s)	558, 1906, 2478, 2734, 2798
NmeAIII	GCCGAG	1 cut(s)	1619
NmuCI	GTSAC	2 cut(s)	486, 664
NsiI	ATGCAT	2 cut(s)	466, 2101
NspV	TTCGAA	2 cut(s)	1190, 1289
OliI	CACNNNNGTG	1 cut(s)	2744
PaeR7I	CTCGAG	1 cut(s)	2771
PciSI	GCTCTTC	1 cut(s)	2060
PctI	GAATGC	4 cut(s)	464, 1137, 1826, 2180
PdmI	GAANNNNTTC	3 cut(s)	404, 2069, 2697
PfoI	TCCNGGA	2 cut(s)	154, 924
PkrI	GCNGC	7 cut(s)	58, 70, 1136, 1592, 2000, 2356, 2569
PleI	GAGTC	8 cut(s)	264, 287, 661, 781, 820, 972, 1024, 2028
PpsI	GAGTC	8 cut(s)	264, 287, 661, 781, 820, 972, 1024, 2028
PpuMI	RGGWCCY	1 cut(s)	526
PsiI	TTATAA	1 cut(s)	1992
Psp124BI	GAGCTC	2 cut(s)	114, 1600
Psp5II	RGGWCCY	1 cut(s)	526
Psp6I	CCWGG	3 cut(s)	154, 366, 696
PspFI	CCCAGC	2 cut(s)	421, 539
PspGI	CCWGG	3 cut(s)	154, 366, 696
PspN4I	GGNNCC	5 cut(s)	558, 1906, 2478, 2734, 2798
PspPI	GGNCC	4 cut(s)	526, 557, 917, 1709
PspPPI	RGGWCCY	1 cut(s)	526
PspXI	VCTCGAGB	1 cut(s)	2771
PsrI	GAACNNNNNNTAC	2 cut(s)	308, 340
PstI	CTGCAG	1 cut(s)	2542
PstNI	CAGNNNCTG	4 cut(s)	59, 71, 2537, 2567
PsuI	RGATCY	2 cut(s)	892, 1281
PvuII	CAGCTG	4 cut(s)	59, 71, 425, 497
RsaI	GTAC	5 cut(s)	1391, 1980, 2297, 2363, 2536
RsaNI	GTAC	5 cut(s)	1390, 1979, 2296, 2362, 2535
RseI	CAYNNNNRTG	4 cut(s)	1441, 1949, 2122, 2744
SacI	GAGCTC	2 cut(s)	114, 1600
SapI	GCTCTTC	1 cut(s)	2060
SaqAI	TTAA	5 cut(s)	101, 377, 1173, 1395, 2834
SatI	GCNGC	7 cut(s)	57, 69, 1135, 1591, 1999, 2355, 2568
Sau96I	GGNCC	4 cut(s)	526, 557, 917, 1709
ScaI	AGTACT	2 cut(s)	1391, 1980
SchI	GAGTC	8 cut(s)	265, 288, 661, 782, 821, 973, 1024, 2028
ScrFI	CCNGG	7 cut(s)	156, 368, 698, 916, 926, 1875, 1909
SduI	GDGCHC	5 cut(s)	114, 1170, 1427, 1600, 2772
SfaNI	GCATC	7 cut(s)	451, 714, 2086, 2126, 2287, 2491, 2515
SfcI	CTRYAG	2 cut(s)	2538, 2580
Sfr274I	CTCGAG	1 cut(s)	2771
SfuI	TTCGAA	2 cut(s)	1190, 1289
SinI	GGWCC	3 cut(s)	526, 557, 1709
SlaI	CTCGAG	1 cut(s)	2771
SmiMI	CAYNNNNRTG	4 cut(s)	1441, 1949, 2122, 2744
SmlI	CTYRAG	7 cut(s)	521, 593, 809, 1385, 1601, 2440, 2771
SmoI	CTYRAG	7 cut(s)	521, 593, 809, 1385, 1601, 2440, 2771
SsiI	CCGC	2 cut(s)	2132, 2480
SspMI	CTAG	9 cut(s)	239, 536, 569, 938, 1587, 1682, 1973, 2189, 2793
SstI	GAGCTC	2 cut(s)	114, 1600
StyD4I	CCNGG	7 cut(s)	154, 366, 696, 914, 924, 1873, 1907
StyI	CCWWGG	4 cut(s)	710, 2088, 2553, 2820
TaaI	ACNGT	8 cut(s)	664, 960, 1556, 1580, 1935, 2020, 2155, 2576
TaiI	ACGT	1 cut(s)	2114
TaqI	TCGA	7 cut(s)	1190, 1289, 2631, 2687, 2772, 2812, 2851
TatI	WGTACW	3 cut(s)	1389, 1978, 2361
Tru1I	TTAA	5 cut(s)	101, 377, 1173, 1395, 2834
Tru9I	TTAA	5 cut(s)	101, 377, 1173, 1395, 2834
TseFI	GTSAC	2 cut(s)	486, 664
TseI	GCWGC	7 cut(s)	56, 68, 1134, 1590, 1998, 2354, 2567
Tsp45I	GTSAC	2 cut(s)	486, 664
TspDTI	ATGAA	8 cut(s)	34, 593, 921, 1305, 1565, 2200, 2424, 2739
VpaK11BI	GGWCC	3 cut(s)	526, 557, 1709
XapI	RAATTY	5 cut(s)	1292, 1465, 2492, 2634, 2659
XbaI	TCTAGA	2 cut(s)	238, 937
XcmI	CCANNNNNNNNNTGG	2 cut(s)	422, 2086
XhoI	CTCGAG	1 cut(s)	2771
XmiI	GTMKAC	3 cut(s)	1115, 1323, 2607
XmnI	GAANNNNTTC	3 cut(s)	404, 2069, 2697
XspI	CTAG	9 cut(s)	239, 536, 569, 938, 1587, 1682, 1973, 2189, 2793
ZraI	GACGTC	1 cut(s)	2112
ZrmI	AGTACT	2 cut(s)	1391, 1980
Zsp2I	ATGCAT	2 cut(s)	466, 2101

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.