Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0014543

Physical Location & Seq

Genomic Coordinates Reverse (-)

11723962 .. 11727456

Gene Structure 3495 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1157800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2901 bp

ATGGAGAATTTCCACTCCTCCTTCTACTTCAACCTTCTCCTCCCTCTTCTCTTCCTCTACGCGGCGGTTTCCGCCGTCGATATCTACTCAGACACCCTCCTGAGCTTAAAATCCGAGCTCGCAGACGTCAATGGCAGCTTGAAAGATTGGATTCTCCCTTCTGCAGAAAACCCACCTGGCAAAATCCACGCATGTTCTTGGTCCGGCGTCAAATGCAACACCAACAACTCCGCCGTCACTGGCATAGACCTCTCCATGAAGAGTCTCGCCGGTGAAATTTCCGGCAACCAATTCGCCGTCTTCACCGACCTTGTGTCACTCAACCTCAGTTACAACTCGTTCTCCGGTCACCTCCCCGCCGGAATTTTCAACCTCACCAACCTCACAACATTAGACATCAGCAGAAACAACTTCTCCGGCCACTTCCCCGTCGGAGTCTCAGCCCTTCAAAACCTCATTGTTCTCGACGCATTCAGCAACAGCTTTTCCGGGTCGCTCCCCGCCGAAATTTCAGAGCTTCAGAACCTCAAAATCCTCAATTTAGCCGGAAGCTACTTCCGGGGACCGATCCCGTTGGAATACGGCGCTTTCCGCCGCCTCGAGTTTATTCATCTCGCCGGGAATTTGATCACCGGAAGCATTCCGCCGGAACTGGGGAAGCTGAAAACAGTGACGCACATGGAAATCGGCTACAATTATTACCACGGAAGCATTCCGTGGCAATTGGGGAACATGACGGAACTCCAGTATCTCGACATTGCCTATGCAAATCTCTCCGGCGCCATCCCAAAATCCCTCGGGGATCTCAACAATCTCGAATCCCTCTTCCTCTTCAGAAACCAGCTGTCTGGGTCGATTCCGGCGGAGCTCAGCAACATCAGAGCTCTCCAAAGCTTGGATCTTTCCGACAATCTGATTTCTGGGTCGATCCCAGAAACCTTTTCGGAGCTGAAAAACCTTAAACTCCTCAGCCTTTTTTACAACGAGATGGCCGGCGAAGTTCCCGACGGAATTGCAGAGCTTCCATCGCTGGAGACGCTGCTTTTGTGGAACAATTACTTCTCCGGCAACCTTCCCCGGAACTTGGGGAGAAACTCGATGCTCGGGTGGGTGGATGTCTCCACAAACAACCTCAGCGGTGGCGTTCCACGGGATATCTGTGCAAATGGCCAGCTCTTTAAGCTGATGCTGTTTTCGAACAACTTCACTGGCGGCCTCTCCACTTCTCTGTCAAACTGTTCGTCTCTGGTTCGTCTTCGACTCGAAGATAACATGTTTTCAGGTGAGATTCCATTGAAATTCAGCAGTCTGACTGATATCACATATGTTGATTTATCTGGGAACAATTTTAGTGGTGGGATTCCAATGGATATTTCAAAAGCTCAAAAGCTTGAGTACTTCAATTTGTCTAGAAATCCAGAATTAGGAGGCACAGTTCCTGCAGAAATGTGGGAATTGCCAAATCTTCACAATTTTTCGGCTTCCTCCTGTGGTTTGATTGGCAACGTTCCTTCATTTGAAAACTGCAGATCGATATATGTTGTTGAATTGAGCAAGAACAGTTTGGAAGGTACTGTTTCGGAAAGTATTTCTAAGTGCCAGGCTCTTGAGAGGATTGATTTAGGAAGCAATAATTTATCCGGTCGTATACCTGAGGAGCTTGCAAGTCTTCCTGCTCTTGGTGTTCTTGACCTGTCACACAATAGCTTCGATGGATCAATACCTAAAAAGTTTGGTTCTTCTTCGACCTTGGTACTTCTAAATGTGTCTTACAATGATGTTTCCGGAATTATTCCATCTGGAAAGGTATTTAGAACAATGGAACAAAGTGCGTTTGTTGGGAATCCGAAACTTTGTGGAGAGCCTCTGAGATCATGTCCTGGTTCAGTTGCTATATTTGGAAGCAGAGGCGTCGGGAAGCTTTTATGGATTCTGCTACTGTGCGCTGTAGTAGTTATGTTTGTTACTCTGTCGGTTATGGGGATATTTTATTTCCGTAGAGAAAGCAAAGGCCAATGGAAGATGATCTCGTTTGATGGACTTCCTCAATTCACAGCAAATGATGTGTTGAAGAGCTTCAGTTCCACAGAATCCATGGATGCATTACCGCCTCTGTCAGCTTCGGTAAGCAAAGCTGTTCTGCCGACGGGAATCACAGTTTCTGTGAAGAAGATTGAATGGGGAGCAAAGAGAATGAGAGTTATGTTGGAATTCGTAACACAAATAGGGAATGCAAGGCATAAGAATTTAATTCGATTGCTCGGAGTTTGCTACAACAATCAACTGGCTTATCTCTTGTACGAGTACTTGCCCAACGGAAACTTGGCTGAAAAGATAAGTGTGAAAAGGGAATGGGCAGCCAAGTACAAAATTGTCATCGGCGTTGCAAAGGGATTGCACTTCCTTCACCACGAATGTTATCCTGCAATTGCACATGGAGATCTGAGGTCAAGGAATATAGTGTTTGACGAAAACATGGAACCTTGTCTGACAGAATTCGGGTTCAAGCATCTGCTCGAGTTGAACAAAGATTCGTTTGCAGCAGCAACTTCCACCTGGGATGCAGGTGATAACATCAGTGTCACAAAAGAGGAGCTATGCAGGGATGTATACAGCTTTGGGGAGATCATGCTGGAGATTCTAAGCCGCGGCAGGTTGACAAATTCTGGCGCAAGTATGCATAGCAAGTCAAGAGAAGTTGTTCTGCGGGAAATCTACAACGAGAACGAAGCTGGTTCCAACATTTCACTGCAAGAAGAGATAAAACTGGTAGTTGAGGTTGCTATGCTTTGCACCAGGAGTAGGCCATCCGATCGACCATCCATGGAAAGTGCATTGAAGCTTTTATCCGAGTCGAAAATCCAGATGAAAAACAACCCAACTTTTGAAGCAGCAGGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001763 GO:0003006 GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009987 GO:0010016 GO:0010065 GO:0010067 GO:0010087 GO:0010089 GO:0010154 GO:0010223 GO:0010346 GO:0016020 GO:0022414 GO:0032501 GO:0032502 GO:0044464 GO:0048316 GO:0048367 GO:0048507 GO:0048508 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0051301 GO:0061458 GO:0071944 GO:1905393

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

967

Amino Acids

105.85

Weight (kDa)

5.66

Isoelectric Point (pI)

33.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_14	PF23598	164 - 280	1e-07	Leucine-rich repeat region
LRR_14	PF23598	284 - 352	1.3e-06	Leucine-rich repeat region
LRR_8	PF13855	295 - 353	9.6e-08	Leucine rich repeat
LRR_8	PF13855	511 - 569	4e-08	Leucine rich repeat
Pkinase	PF00069	708 - 947	1.8e-21	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	716 - 946	2.6e-24	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014543)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G51350
fragaria_vesca	FvH4_1g01520
malus_domestica	MD02G1012000.v1.1 MD15G1157800.v1.1
prunus_persica	Prupe.7G253000_v2.0.a1
pyrus_communis	pycom02g01130 pycom15g14160
rosa_chinensis	RchiOBHm_Chr2g0086441
rosa_laevigata	RLG00000015759
rosa_multiflora	Rmu_sc0005127.1_g000003
rosa_roxburghii	Rroxscaffold_2G00154570
rosa_rugosa	Rorug01G0467300
rosa_samantha	Rh2AG018100 Rh2BG017600 Rh2CG018500 Rh2DG019400
rosa_wichuraiana	Rw2G001500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2555
AatII	GACGTC	1 cut(s)	129
Acc36I	ACCTGC	2 cut(s)	2555, 2643
AccB1I	GGYRCC	1 cut(s)	779
AccB7I	CCANNNNNTGG	1 cut(s)	895
AccI	GTMKAC	2 cut(s)	1648, 2610
AccII	CGCG	2 cut(s)	62, 2649
AccIII	TCCGGA	1 cut(s)	1784
AclI	AACGTT	1 cut(s)	1506
AclWI	GGATC	5 cut(s)	562, 810, 906, 922, 1723
AcoI	YGGCCR	3 cut(s)	418, 990, 1168
AcuI	CTGAAG	3 cut(s)	503, 817, 2062
AcyI	GRCGYC	4 cut(s)	126, 207, 780, 1911
AfaI	GTAC	6 cut(s)	1397, 1573, 1755, 2300, 2306, 2366
AfiI	CCNNNNNNNGG	7 cut(s)	61, 653, 895, 1084, 1424, 1679, 2802
AflIII	ACRYGT	1 cut(s)	1272
AhdI	GACNNNNNGTC	1 cut(s)	313
AjnI	CCWGG	5 cut(s)	175, 1599, 1879, 2555, 2795
AjuI	GAANNNNNNNTTGG	2 cut(s)	1357, 1389
AloI	GAACNNNNNNTCC	2 cut(s)	1419, 1451
Alw21I	GWGCWC	3 cut(s)	120, 870, 886
Alw26I	GTCTC	5 cut(s)	269, 442, 1028, 1123, 1248
AlwI	GGATC	5 cut(s)	562, 810, 906, 922, 1723
AlwNI	CAGNNNCTG	2 cut(s)	1439, 2162
Ama87I	CYCGRG	4 cut(s)	599, 797, 1103, 2516
Aor13HI	TCCGGA	1 cut(s)	1784
AoxI	GGCC	6 cut(s)	418, 990, 1168, 1213, 2011, 2804
ApeKI	GCWGC	6 cut(s)	135, 1039, 2357, 2540, 2543, 2891
ArsI	GACNNNNNNTTYG	4 cut(s)	1027, 1059, 1226, 1258
Asp700I	GAANNNNTTC	3 cut(s)	1202, 2075, 2700
AspLEI	GCGC	4 cut(s)	587, 782, 1946, 2672
AspS9I	GGNCC	2 cut(s)	201, 563
AsuC2I	CCSGG	4 cut(s)	490, 560, 619, 1078
AsuHPI	GGTGA	8 cut(s)	284, 295, 341, 367, 622, 1295, 2399, 2579
AsuII	TTCGAA	1 cut(s)	1196
AvaI	CYCGRG	4 cut(s)	599, 797, 1103, 2516
AvaII	GGWCC	2 cut(s)	201, 563
AxyI	CCTNAGG	1 cut(s)	1653
BaeI	ACNNNNGTAYC	2 cut(s)	731, 764
BalI	TGGCCA	1 cut(s)	1170
BanI	GGYRCC	1 cut(s)	779
BanII	GRGCYC	3 cut(s)	120, 870, 886
BbsI	GAAGAC	3 cut(s)	292, 1247, 1661
Bbv12I	GWGCWC	3 cut(s)	120, 870, 886
BbvCI	CCTCAGC	2 cut(s)	968, 1133
BbvI	GCAGC	5 cut(s)	147, 1026, 2369, 2552, 2555
BccI	CCATC	8 cut(s)	791, 982, 1033, 1706, 1804, 2030, 2815, 2827
BceAI	ACGGC	4 cut(s)	59, 218, 281, 598
BcgI	CGANNNNNNTGC	6 cut(s)	274, 308, 995, 1029, 1894, 1928
BciT130I	CCWGG	5 cut(s)	177, 1601, 1881, 2557, 2797
BclI	TGATCA	1 cut(s)	627
BcnI	CCSGG	4 cut(s)	490, 560, 619, 1078
BcoDI	GTCTC	5 cut(s)	269, 442, 1028, 1123, 1248
BfaI	CTAG	1 cut(s)	1410
BfmI	CTRYAG	4 cut(s)	162, 1440, 1525, 1947
BfoI	RGCGCY	2 cut(s)	588, 783
BfuAI	ACCTGC	2 cut(s)	2555, 2643
BglII	AGATCT	1 cut(s)	2440
BlpI	GCTNAGC	1 cut(s)	869
BmcAI	AGTACT	2 cut(s)	1397, 2306
Bme1390I	CCNGG	9 cut(s)	177, 490, 560, 619, 1078, 1601, 1881, 2557, 2797
Bme18I	GGWCC	2 cut(s)	201, 563
BmeRI	GACNNNNNGTC	1 cut(s)	313
BmeT110I	CYCGRG	4 cut(s)	599, 797, 1103, 2516
BmgT120I	GGNCC	2 cut(s)	201, 563
BmiI	GGNNCC	4 cut(s)	564, 781, 2481, 2737
BmrFI	CCNGG	9 cut(s)	177, 490, 560, 619, 1078, 1601, 1881, 2557, 2797
BmrI	ACTGGG	1 cut(s)	662
BmsI	GCATC	5 cut(s)	1089, 1176, 2089, 2518, 2551
BmuI	ACTGGG	1 cut(s)	662
BpiI	GAAGAC	3 cut(s)	292, 1247, 1661
BpmI	CTGGAG	3 cut(s)	728, 1052, 2654
Bpu10I	CCTNAGC	3 cut(s)	101, 968, 1133
Bpu1102I	GCTNAGC	1 cut(s)	869
Bpu14I	TTCGAA	1 cut(s)	1196
BpuEI	CTTGAG	2 cut(s)	1412, 1628
BpuMI	CCSGG	4 cut(s)	490, 560, 619, 1078
Bsa29I	ATCGAT	1 cut(s)	1532
BsaHI	GRCGYC	4 cut(s)	126, 207, 780, 1911
BsaWI	WCCGGW	4 cut(s)	344, 632, 1640, 1784
BsaXI	ACNNNNNCTCC	6 cut(s)	326, 356, 398, 428, 1419, 1449
Bsc4I	CCNNNNNNNGG	7 cut(s)	61, 653, 895, 1084, 1424, 1679, 2802
Bse118I	RCCGGY	2 cut(s)	269, 992
Bse1I	ACTGG	6 cut(s)	244, 657, 745, 1213, 2289, 2772
Bse21I	CCTNAGG	1 cut(s)	1653
Bse3DI	GCAATG	1 cut(s)	756
BseAI	TCCGGA	1 cut(s)	1784
BseBI	CCWGG	5 cut(s)	177, 1601, 1881, 2557, 2797
BseCI	ATCGAT	1 cut(s)	1532
BseGI	GGATG	7 cut(s)	783, 1120, 2104, 2566, 2611, 2807, 2819
BseLI	CCNNNNNNNGG	7 cut(s)	61, 653, 895, 1084, 1424, 1679, 2802
BseMI	GCAATG	1 cut(s)	756
BseNI	ACTGG	6 cut(s)	244, 657, 745, 1213, 2289, 2772
BseRI	GAGGAG	5 cut(s)	7, 29, 956, 1670, 2606
BseXI	GCAGC	5 cut(s)	147, 1026, 2369, 2552, 2555
Bsh1236I	CGCG	2 cut(s)	62, 2649
Bsh1285I	CGRYCG	2 cut(s)	1645, 2815
BshFI	GGCC	6 cut(s)	420, 992, 1170, 1215, 2013, 2806
BshNI	GGYRCC	1 cut(s)	779
BshVI	ATCGAT	1 cut(s)	1532
BsiEI	CGRYCG	2 cut(s)	1645, 2815
BsiHKAI	GWGCWC	3 cut(s)	120, 870, 886
BsiHKCI	CYCGRG	4 cut(s)	599, 797, 1103, 2516
BslFI	GGGAC	1 cut(s)	576
BslI	CCNNNNNNNGG	7 cut(s)	61, 653, 895, 1084, 1424, 1679, 2802
BsmAI	GTCTC	5 cut(s)	269, 442, 1028, 1123, 1248
BsmBI	CGTCTC	2 cut(s)	1028, 1248
BsmFI	GGGAC	1 cut(s)	576
BsmI	GAATGC	4 cut(s)	470, 639, 711, 2236
BsnI	GGCC	6 cut(s)	420, 992, 1170, 1215, 2013, 2806
BsoBI	CYCGRG	4 cut(s)	599, 797, 1103, 2516
Bsp119I	TTCGAA	1 cut(s)	1196
Bsp1286I	GDGCHC	3 cut(s)	120, 870, 886
Bsp13I	TCCGGA	1 cut(s)	1784
Bsp1720I	GCTNAGC	1 cut(s)	869
Bsp19I	CCATGG	2 cut(s)	2094, 2823
BspANI	GGCC	6 cut(s)	420, 992, 1170, 1215, 2013, 2806
BspDI	ATCGAT	1 cut(s)	1532
BspEI	TCCGGA	1 cut(s)	1784
BspFNI	CGCG	2 cut(s)	62, 2649
BspLI	GGNNCC	4 cut(s)	564, 781, 2481, 2737
BspMAI	CTGCAG	3 cut(s)	166, 1444, 1529
BspMI	ACCTGC	2 cut(s)	2555, 2643
BspPI	GGATC	5 cut(s)	562, 810, 906, 922, 1723
BspQI	GCTCTTC	1 cut(s)	2066
BspT104I	TTCGAA	1 cut(s)	1196
BspT107I	GGYRCC	1 cut(s)	779
BsrDI	GCAATG	1 cut(s)	756
BsrFI	RCCGGY	2 cut(s)	269, 992
BsrI	ACTGG	6 cut(s)	244, 657, 745, 1213, 2289, 2772
BssAI	RCCGGY	2 cut(s)	269, 992
BssNAI	GTATAC	2 cut(s)	1649, 2611
BssNI	GRCGYC	4 cut(s)	126, 207, 780, 1911
BssT1I	CCWWGG	3 cut(s)	1749, 2094, 2823
Bst1107I	GTATAC	2 cut(s)	1649, 2611
Bst2UI	CCWGG	5 cut(s)	177, 1601, 1881, 2557, 2797
Bst4CI	ACNGT	7 cut(s)	670, 1238, 1435, 1562, 1576, 1941, 2158
Bst6I	CTCTTC	7 cut(s)	51, 56, 254, 830, 836, 2066, 2751
BstACI	GRCGYC	4 cut(s)	126, 207, 780, 1911
BstBI	TTCGAA	1 cut(s)	1196
BstC8I	GCNNGC	4 cut(s)	120, 994, 1172, 1662
BstDSI	CCRYGG	6 cut(s)	703, 716, 1148, 2094, 2647, 2823
BstEII	GGTNACC	1 cut(s)	347
BstF5I	GGATG	7 cut(s)	783, 1120, 2104, 2566, 2611, 2807, 2819
BstFNI	CGCG	2 cut(s)	62, 2649
BstH2I	RGCGCY	2 cut(s)	588, 783
BstHHI	GCGC	4 cut(s)	587, 782, 1946, 2672
BstMAI	GTCTC	5 cut(s)	269, 442, 1028, 1123, 1248
BstMCI	CGRYCG	2 cut(s)	1645, 2815
BstMWI	GCNNNNNNNGC	6 cut(s)	71, 213, 591, 1027, 1036, 1180
BstNI	CCWGG	5 cut(s)	177, 1601, 1881, 2557, 2797
BstNSI	RCATGY	2 cut(s)	195, 1276
BstPI	GGTNACC	1 cut(s)	347
BstSCI	CCNGG	9 cut(s)	175, 488, 558, 617, 1076, 1599, 1879, 2555, 2795
BstSFI	CTRYAG	4 cut(s)	162, 1440, 1525, 1947
BstUI	CGCG	2 cut(s)	62, 2649
BstV1I	GCAGC	5 cut(s)	147, 1026, 2369, 2552, 2555
BstV2I	GAAGAC	3 cut(s)	292, 1247, 1661
BstX2I	RGATCY	3 cut(s)	802, 898, 2440
BstXI	CCANNNNNNTGG	1 cut(s)	848
BstYI	RGATCY	3 cut(s)	802, 898, 2440
BstZ17I	GTATAC	2 cut(s)	1649, 2611
Bsu15I	ATCGAT	1 cut(s)	1532
Bsu36I	CCTNAGG	1 cut(s)	1653
BsuRI	GGCC	6 cut(s)	420, 992, 1170, 1215, 2013, 2806
BsuTUI	ATCGAT	1 cut(s)	1532
BtgI	CCRYGG	6 cut(s)	703, 716, 1148, 2094, 2647, 2823
BtgZI	GCGATG	1 cut(s)	1011
BtsCI	GGATG	7 cut(s)	783, 1120, 2104, 2566, 2611, 2807, 2819
BtsI	GCAGTG	1 cut(s)	2747
BtsIMutI	CAGTG	5 cut(s)	237, 675, 1206, 2584, 2747
BveI	ACCTGC	2 cut(s)	2555, 2643
Cac8I	GCNNGC	4 cut(s)	120, 994, 1172, 1662
CaiI	CAGNNNCTG	2 cut(s)	1439, 2162
CfoI	GCGC	4 cut(s)	587, 782, 1946, 2672
Cfr10I	RCCGGY	2 cut(s)	269, 992
Cfr13I	GGNCC	2 cut(s)	201, 563
Cfr42I	CCGCGG	1 cut(s)	2650
ClaI	ATCGAT	1 cut(s)	1532
CseI	GACGC	5 cut(s)	196, 476, 682, 1045, 1900
Csp6I	GTAC	6 cut(s)	1396, 1572, 1754, 2299, 2305, 2365
CspCI	CAANNNNNGTGG	2 cut(s)	1333, 1368
CviQI	GTAC	6 cut(s)	1396, 1572, 1754, 2299, 2305, 2365
DinI	GGCGCC	1 cut(s)	781
DriI	GACNNNNNGTC	1 cut(s)	313
EaeI	YGGCCR	3 cut(s)	418, 990, 1168
Eam1104I	CTCTTC	7 cut(s)	51, 56, 254, 830, 836, 2066, 2751
Eam1105I	GACNNNNNGTC	1 cut(s)	313
EarI	CTCTTC	7 cut(s)	51, 56, 254, 830, 836, 2066, 2751
EciI	GGCGGA	5 cut(s)	61, 220, 581, 633, 878
Ecl136II	GAGCTC	3 cut(s)	118, 868, 884
Eco130I	CCWWGG	3 cut(s)	1749, 2094, 2823
Eco24I	GRGCYC	3 cut(s)	120, 870, 886
Eco32I	GATATC	3 cut(s)	82, 1156, 1318
Eco47I	GGWCC	2 cut(s)	201, 563
Eco53kI	GAGCTC	3 cut(s)	118, 868, 884
Eco57I	CTGAAG	3 cut(s)	503, 817, 2062
Eco81I	CCTNAGG	1 cut(s)	1653
Eco88I	CYCGRG	4 cut(s)	599, 797, 1103, 2516
Eco91I	GGTNACC	1 cut(s)	347
EcoICRI	GAGCTC	3 cut(s)	118, 868, 884
EcoO65I	GGTNACC	1 cut(s)	347
EcoRI	GAATTC	2 cut(s)	2210, 2495
EcoRII	CCWGG	5 cut(s)	175, 1599, 1879, 2555, 2795
EcoRV	GATATC	3 cut(s)	82, 1156, 1318
EcoT14I	CCWWGG	3 cut(s)	1749, 2094, 2823
EcoT22I	ATGCAT	2 cut(s)	2104, 2682
EcoT38I	GRGCYC	3 cut(s)	120, 870, 886
EgeI	GGCGCC	1 cut(s)	781
EheI	GGCGCC	1 cut(s)	781
ErhI	CCWWGG	3 cut(s)	1749, 2094, 2823
Esp3I	CGTCTC	2 cut(s)	1028, 1248
FaqI	GGGAC	1 cut(s)	576
FauI	CCCGC	3 cut(s)	364, 508, 2700
FauNDI	CATATG	1 cut(s)	1324
FbaI	TGATCA	1 cut(s)	627
FblI	GTMKAC	2 cut(s)	1648, 2610
FokI	GGATG	7 cut(s)	770, 1127, 2111, 2573, 2618, 2794, 2806
FriOI	GRGCYC	3 cut(s)	120, 870, 886
FspBI	CTAG	1 cut(s)	1410
GlaI	GCGC	4 cut(s)	586, 781, 1945, 2671
GsuI	CTGGAG	3 cut(s)	728, 1052, 2654
HaeII	RGCGCY	2 cut(s)	588, 783
HaeIII	GGCC	6 cut(s)	420, 992, 1170, 1215, 2013, 2806
HgaI	GACGC	5 cut(s)	196, 476, 682, 1045, 1900
HhaI	GCGC	4 cut(s)	587, 782, 1946, 2672
Hin1I	GRCGYC	4 cut(s)	126, 207, 780, 1911
Hin6I	GCGC	4 cut(s)	585, 780, 1944, 2670
HinP1I	GCGC	4 cut(s)	585, 780, 1944, 2670
HincII	GTYRAC	1 cut(s)	2658
HindII	GTYRAC	1 cut(s)	2658
HindIII	AAGCTT	4 cut(s)	892, 1388, 1919, 2840
HphI	GGTGA	8 cut(s)	284, 295, 341, 367, 622, 1295, 2399, 2579
Hpy166II	GTNNAC	3 cut(s)	1649, 2611, 2658
Hpy8I	GTNNAC	3 cut(s)	1649, 2611, 2658
Hpy99I	CGWCG	6 cut(s)	80, 434, 470, 1010, 1916, 2149
HpyAV	CCTTC	8 cut(s)	31, 44, 168, 455, 1082, 1521, 1562, 2414
HpyCH4III	ACNGT	7 cut(s)	670, 1238, 1435, 1562, 1576, 1941, 2158
HpyCH4IV	ACGT	2 cut(s)	126, 1506
HpyF10VI	GCNNNNNNNGC	6 cut(s)	71, 213, 591, 1027, 1036, 1180
HpySE526I	ACGT	2 cut(s)	126, 1506
Hsp92I	GRCGYC	4 cut(s)	126, 207, 780, 1911
HspAI	GCGC	4 cut(s)	585, 780, 1944, 2670
KasI	GGCGCC	1 cut(s)	779
Kpn2I	TCCGGA	1 cut(s)	1784
KroI	GCCGGC	1 cut(s)	992
KroNI	GCCGGC	1 cut(s)	994
Ksp22I	TGATCA	1 cut(s)	627
KspI	CCGCGG	1 cut(s)	2650
LguI	GCTCTTC	1 cut(s)	2066
LmnI	GCTCC	6 cut(s)	501, 865, 946, 1657, 2183, 2593
Lsp1109I	GCAGC	5 cut(s)	147, 1026, 2369, 2552, 2555
LweI	GCATC	5 cut(s)	1089, 1176, 2089, 2518, 2551
MaeI	CTAG	1 cut(s)	1410
MaeII	ACGT	2 cut(s)	126, 1506
MaeIII	GTNAC	9 cut(s)	235, 315, 329, 347, 670, 1695, 1963, 2215, 2581
MfeI	CAATTG	2 cut(s)	722, 2427
MflI	RGATCY	3 cut(s)	802, 898, 2440
MhlI	GDGCHC	3 cut(s)	120, 870, 886
MlsI	TGGCCA	1 cut(s)	1170
MluNI	TGGCCA	1 cut(s)	1170
Mly113I	GGCGCC	1 cut(s)	780
MlyI	GAGTC	4 cut(s)	271, 444, 1254, 2861
MmeI	TCCRAC	5 cut(s)	412, 555, 930, 2187, 2763
Mox20I	TGGCCA	1 cut(s)	1170
Mph1103I	ATGCAT	2 cut(s)	2104, 2682
MroI	TCCGGA	1 cut(s)	1784
MroNI	GCCGGC	1 cut(s)	992
MroXI	GAANNNNTTC	3 cut(s)	1202, 2075, 2700
MscI	TGGCCA	1 cut(s)	1170
MseI	TTAA	4 cut(s)	107, 960, 1179, 2249
Msp20I	TGGCCA	1 cut(s)	1170
MspA1I	CMGCKG	3 cut(s)	844, 1137, 2649
MspR9I	CCNGG	9 cut(s)	177, 490, 560, 619, 1078, 1601, 1881, 2557, 2797
MunI	CAATTG	2 cut(s)	722, 2427
Mva1269I	GAATGC	4 cut(s)	470, 639, 711, 2236
MvaI	CCWGG	5 cut(s)	177, 1601, 1881, 2557, 2797
MvnI	CGCG	2 cut(s)	62, 2649
MwoI	GCNNNNNNNGC	6 cut(s)	71, 213, 591, 1027, 1036, 1180
NaeI	GCCGGC	1 cut(s)	994
NarI	GGCGCC	1 cut(s)	780
NciI	CCSGG	4 cut(s)	490, 560, 619, 1078
NcoI	CCATGG	2 cut(s)	2094, 2823
NdeI	CATATG	1 cut(s)	1324
NgoMIV	GCCGGC	1 cut(s)	992
NlaIV	GGNNCC	4 cut(s)	564, 781, 2481, 2737
NmuCI	GTSAC	6 cut(s)	235, 315, 347, 670, 1695, 2581
NsiI	ATGCAT	2 cut(s)	2104, 2682
NspI	RCATGY	2 cut(s)	195, 1276
NspV	TTCGAA	1 cut(s)	1196
PaeR7I	CTCGAG	2 cut(s)	599, 2516
PaqCI	CACCTGC	1 cut(s)	2555
PciI	ACATGT	1 cut(s)	1272
PciSI	GCTCTTC	1 cut(s)	2066
PctI	GAATGC	4 cut(s)	470, 639, 711, 2236
PdiI	GCCGGC	1 cut(s)	994
PdmI	GAANNNNTTC	3 cut(s)	1202, 2075, 2700
PflMI	CCANNNNNTGG	1 cut(s)	895
Ple19I	CGATCG	1 cut(s)	2815
PleI	GAGTC	4 cut(s)	270, 443, 1254, 2860
PluTI	GGCGCC	1 cut(s)	783
PpsI	GAGTC	4 cut(s)	270, 443, 1254, 2860
PscI	ACATGT	1 cut(s)	1272
Psp124BI	GAGCTC	3 cut(s)	120, 870, 886
Psp1406I	AACGTT	1 cut(s)	1506
Psp6I	CCWGG	5 cut(s)	175, 1599, 1879, 2555, 2795
PspEI	GGTNACC	1 cut(s)	347
PspGI	CCWGG	5 cut(s)	175, 1599, 1879, 2555, 2795
PspN4I	GGNNCC	4 cut(s)	564, 781, 2481, 2737
PspPI	GGNCC	2 cut(s)	201, 563
PspXI	VCTCGAGB	2 cut(s)	599, 2516
PstI	CTGCAG	3 cut(s)	166, 1444, 1529
PstNI	CAGNNNCTG	2 cut(s)	1439, 2162
PsuI	RGATCY	3 cut(s)	802, 898, 2440
PvuI	CGATCG	1 cut(s)	2815
PvuII	CAGCTG	1 cut(s)	844
RsaI	GTAC	6 cut(s)	1397, 1573, 1755, 2300, 2306, 2366
RsaNI	GTAC	6 cut(s)	1396, 1572, 1754, 2299, 2305, 2365
SacI	GAGCTC	3 cut(s)	120, 870, 886
SacII	CCGCGG	1 cut(s)	2650
SapI	GCTCTTC	1 cut(s)	2066
SaqAI	TTAA	4 cut(s)	107, 960, 1179, 2249
Sau96I	GGNCC	2 cut(s)	201, 563
ScaI	AGTACT	2 cut(s)	1397, 2306
SchI	GAGTC	4 cut(s)	271, 444, 1254, 2861
ScrFI	CCNGG	9 cut(s)	177, 490, 560, 619, 1078, 1601, 1881, 2557, 2797
SduI	GDGCHC	3 cut(s)	120, 870, 886
SfaNI	GCATC	5 cut(s)	1089, 1176, 2089, 2518, 2551
SfcI	CTRYAG	4 cut(s)	162, 1440, 1525, 1947
SfoI	GGCGCC	1 cut(s)	781
Sfr274I	CTCGAG	2 cut(s)	599, 2516
Sfr303I	CCGCGG	1 cut(s)	2650
SfuI	TTCGAA	1 cut(s)	1196
SgrAI	CRCCGGYG	1 cut(s)	269
SgrBI	CCGCGG	1 cut(s)	2650
SinI	GGWCC	2 cut(s)	201, 563
SlaI	CTCGAG	2 cut(s)	599, 2516
SmlI	CTYRAG	4 cut(s)	599, 1391, 1607, 2516
SmoI	CTYRAG	4 cut(s)	599, 1391, 1607, 2516
SspDI	GGCGCC	1 cut(s)	779
SspMI	CTAG	1 cut(s)	1410
SstI	GAGCTC	3 cut(s)	120, 870, 886
StyD4I	CCNGG	9 cut(s)	175, 488, 558, 617, 1076, 1599, 1879, 2555, 2795
StyI	CCWWGG	3 cut(s)	1749, 2094, 2823
TaaI	ACNGT	7 cut(s)	670, 1238, 1435, 1562, 1576, 1941, 2158
TaiI	ACGT	2 cut(s)	129, 1509
TaqII	GACCGA	1 cut(s)	580
TatI	WGTACW	3 cut(s)	1395, 2304, 2364
TauI	GCSGC	5 cut(s)	65, 597, 1215, 2649, 2652
Tru1I	TTAA	4 cut(s)	107, 960, 1179, 2249
Tru9I	TTAA	4 cut(s)	107, 960, 1179, 2249
TscAI	CASTG	5 cut(s)	244, 675, 1213, 2584, 2754
TseFI	GTSAC	6 cut(s)	235, 315, 347, 670, 1695, 2581
TseI	GCWGC	6 cut(s)	135, 1039, 2357, 2540, 2543, 2891
Tsp45I	GTSAC	6 cut(s)	235, 315, 347, 670, 1695, 2581
TspDTI	ATGAA	4 cut(s)	272, 599, 1503, 2882
TspGWI	ACGGA	6 cut(s)	705, 720, 752, 1023, 1985, 2331
TspRI	CASTG	5 cut(s)	244, 675, 1213, 2584, 2754
Van91I	CCANNNNNTGG	1 cut(s)	895
VpaK11BI	GGWCC	2 cut(s)	201, 563
XbaI	TCTAGA	1 cut(s)	1409
XceI	RCATGY	2 cut(s)	195, 1276
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2092, 2320
XhoI	CTCGAG	2 cut(s)	599, 2516
XmiI	GTMKAC	2 cut(s)	1648, 2610
XmnI	GAANNNNTTC	3 cut(s)	1202, 2075, 2700
XspI	CTAG	1 cut(s)	1410
ZraI	GACGTC	1 cut(s)	127
ZrmI	AGTACT	2 cut(s)	1397, 2306
Zsp2I	ATGCAT	2 cut(s)	2104, 2682

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.